KR20230088828A - Cas9-rnp 및 기타 핵단백질 카고의 형질도입을 위해 적합화된 최소 길이의 셔틀제 펩타이드 및 그의 변이체 - Google Patents

Cas9-rnp 및 기타 핵단백질 카고의 형질도입을 위해 적합화된 최소 길이의 셔틀제 펩타이드 및 그의 변이체 Download PDF

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KR20230088828A
KR20230088828A KR1020237017143A KR20237017143A KR20230088828A KR 20230088828 A KR20230088828 A KR 20230088828A KR 1020237017143 A KR1020237017143 A KR 1020237017143A KR 20237017143 A KR20237017143 A KR 20237017143A KR 20230088828 A KR20230088828 A KR 20230088828A
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데이비드 과이
스테파니 할리
자비에 바베우
토마스 델'주다이스
장 파스칼 레페티-스토파즈
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펠단 바이오 인코포레이티드
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    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4723—Cationic antimicrobial peptides, e.g. defensins
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    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
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    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/08—Linear peptides containing only normal peptide links having 12 to 20 amino acids
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
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    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
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    • C07K2319/00—Fusion polypeptide
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    • C07K2319/10—Fusion polypeptide containing a localisation/targetting motif containing a tag for extracellular membrane crossing, e.g. TAT or VP22
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
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    • C12N2320/00—Applications; Uses
    • C12N2320/30—Special therapeutic applications
    • C12N2320/32—Special delivery means, e.g. tissue-specific

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  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
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KR1020237017143A 2020-10-22 2021-10-22 Cas9-rnp 및 기타 핵단백질 카고의 형질도입을 위해 적합화된 최소 길이의 셔틀제 펩타이드 및 그의 변이체 Pending KR20230088828A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202063104340P 2020-10-22 2020-10-22
US63/104,340 2020-10-22
PCT/CA2021/051490 WO2022082315A1 (en) 2020-10-22 2021-10-22 Shuttle agent peptides of minimal lengh and variants thereof adapted for transduction of cas9-rnp and other nucleoprotein cargos

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KR20230088828A true KR20230088828A (ko) 2023-06-20

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KR1020237017143A Pending KR20230088828A (ko) 2020-10-22 2021-10-22 Cas9-rnp 및 기타 핵단백질 카고의 형질도입을 위해 적합화된 최소 길이의 셔틀제 펩타이드 및 그의 변이체

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US (1) US20230399661A1 (https=)
EP (1) EP4232590A4 (https=)
JP (1) JP2023547405A (https=)
KR (1) KR20230088828A (https=)
CN (1) CN116615227A (https=)
AU (1) AU2021366976A1 (https=)
CA (1) CA3196066A1 (https=)
IL (1) IL302227A (https=)
WO (1) WO2022082315A1 (https=)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110291100B (zh) 2016-10-12 2024-06-11 费尔丹生物公司 用于将多肽负荷从细胞外空间递送至靶真核细胞的胞质溶胶和/或细胞核的合理设计的合成肽穿梭剂，其用途、与其相关的方法和试剂盒
CA3040645A1 (en) 2019-04-18 2020-10-18 Feldan Bio Inc. Peptide-based non-proteinaceous cargo delivery
WO2024206311A1 (en) * 2023-03-31 2024-10-03 The Trustees Of The University Of Pennsylvania Hybrid antimicrobial peptides

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2981716C (en) * 2015-04-10 2022-04-12 Feldan Bio Inc. Polypeptide-based shuttle agents for improving the transduction efficiency of polypeptide cargos to the cytosol of target eukaryotic cells, uses thereof, methods and kits relating to same
CN110291100B (zh) * 2016-10-12 2024-06-11 费尔丹生物公司 用于将多肽负荷从细胞外空间递送至靶真核细胞的胞质溶胶和/或细胞核的合理设计的合成肽穿梭剂，其用途、与其相关的方法和试剂盒
CA3040645A1 (en) * 2019-04-18 2020-10-18 Feldan Bio Inc. Peptide-based non-proteinaceous cargo delivery

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CA3196066A1 (en) 2022-04-28
EP4232590A4 (en) 2025-03-05
IL302227A (en) 2023-06-01
EP4232590A1 (en) 2023-08-30
US20230399661A1 (en) 2023-12-14
WO2022082315A1 (en) 2022-04-28
AU2021366976A1 (en) 2023-06-08
JP2023547405A (ja) 2023-11-10
CN116615227A (zh) 2023-08-18
WO2022082315A8 (en) 2022-08-04

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