KR20230087770A - 과수 화상병에 특이적인 신규 박테리오파지 및 이의 이용 - Google Patents
과수 화상병에 특이적인 신규 박테리오파지 및 이의 이용 Download PDFInfo
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Abstract
본 명세서는 기탁번호 KACC 97037P인 박테리오파지에 관한 것으로, 상기 박테리오파지는 Erwinia amylovora(에르위니아 아밀로보라)에 대해 특이적인 용균활성을 보여, 과수 화상병에 방제 효과가 있으며, 과수 화상병 방제를 위한 미생물 농약으로 활용될 수 있고, 항생제 대신 사용될 수 있으므로, 항생제 저항성 균주의 출현을 막고 환경에 영향을 끼치지 않아 안전하고 효과적인 과수화상병 방제를 기대할 수 있다.
Description
본 명세서는 과수 화상병균에 특이적이고 강력한 용균 활성을 가지는 박테리오파지, 상기 박테리오파지를 포함하는 농약 조성물, 및 이를 이용한 화상병 방제방법에 관한 것이다.
과수 화상병(fire blight)은 대표적인 세균성 식물 검역병 중의 하나로, 에르위니아 아밀로보라(Erwinia amylovora)가 그 원인균으로 알려져있다. 과수 화상병은 배 또는 사과나무 등의 꽃, 가지, 열매를 까맣게 변화시켜 불타 화상입은 것처럼 검게 말라죽는 증상을 보인다. 에르위니아 아밀로보라는 곤충이나 비바람에 의해 전염되어 꽃, 꿀샘, 기공, 피목, 상처 등을 통해 기주체로 침입하기 때문에 전염성이 높고 배나무, 사과나무, 살구나무 등 70여종의 식물을 침해할 수 있다는 특징이 있어 세계적으로 화상병에 대한 피해가 발생하고 있다.
과수 화상병은 확산 속도가 빠른편이기 때문에, 한 그루에서만 증상이 나타나도 과수원 전체를 폐원해야하며, 반경 100m 이내의 과일나무를 뿌리째 뽑아서 태워 땅에 묻는 방식으로 폐기해야한다. 이 때문에, 과수 화상병은 당해의 수확을 망칠 뿐만 아니라, 농가에 장기간동안 피해를 줄 수 있다.
과수 화상병에 대한 대응방법으로서 토양 수분을 감소시키고 비료 영양소의 균형을 유지함으로써 화상병의 감염 및 확산을 제어할 수 있는 방법, 감염된 과수나 검게 변한 가지를 병원균이 휴지 기간을 갖는 겨울에 제거함으로써 화상병을 치료하는 방법 등이 알려져 있으나, 본질적인 질병 발생을 제거할 수 없다.
본 발명자들은 에르위니아 아밀로보라의 방제 방법으로서 박테리오파지를 이용한 방제(치료) 방법인 파지 테라피를 연구한 결과, 배 과수원의 물로부터 분리한 신규 박테리오파지가 에르위니아 아밀로보라에 대해 강한 용균성을 보임을 발견하였다.
본 명세서는 에르위니아 아밀로보라(Erwinia amylovora)에 대해 특이적이고 강력한 용균 활성을 가지는 박테리오파지를 제공하고자 한다.
또한, 본 명세서는 상기 박테리오파지를 포함하는 농약 조성물, 구체적으로는 과수 화상병 방제용 농약 조성물을 제공하고자 한다.
또한, 본 명세서는 상기 박테리오파지를 이용한 화상병 방제방법을 제공하고자 한다.
본 명세서는 에르위니아 아밀로보라(Erwinia amylovora)에 특이적인 용균활성을 갖는, 기탁번호 KACC 97037P인 박테리오파지를 제공한다.
본 발명의 박테리오파지는 서열번호 1의 염기서열을 갖는 것을 특징으로 한다.
본 발명의 박테리오파지는 20℃ 내지 60℃에서 활성을 갖는 것을 특징으로 한다.
본 발명의 박테리오파지는 pH 3 내지 12에서 활성을 갖는 것을 특징으로 한다.
또 하나의 실시상태에 따르면, 본 명세서는 상기 박테리오파지를 유효성분으로 포함하는 농약 조성물을 제공한다.
본 발명의 농약 조성물에 포함된 박테리오파지는 과수 화상병(fire blight)의 예방 또는 치료에 사용되는 것을 특징으로 한다.
또 하나의 실시상태에 따르면, 본 명세서는 상기 박테리오파지를 유효성분으로 포함하는 항생용 조성물을 제공한다.또 하나의 실시상태에 따르면, 본 명세서는 상기 박테리오파지를 유효성분으로 포함하는 소독제를 제공한다.
또 하나의 실시상태에 따르면, 본 명세서는 상기 박테리오파지를 유효성분으로 포함하는 세척제를 제공한다.
또 하나의 실시상태에 따르면, 본 명세서는 상기 박테리오파지를 사용하여 과수 화상병을 방제하는 방법를 제공한다.
또 하나의 실시상태에 따르면, 본 명세서는 상기 박테리오파지의, 과수 화상병(fire blight)의 예방 또는 치료에 사용하기 위한 용도를 제공한다.
또 하나의 실시상태에 따르면, 본 명세서는 상기 박테리오파지의, 과수 화상병(fire blight)의 예방 또는 치료용 농약 조성물의 제조에 사용하기 위한 용도를 제공한다.
본 발명에서 새롭게 분리된, 기탁번호 KACC 97037P인 박테리오파지는 에르위니아 아밀로보라(Erwinia amylovora)에 대한 특이적인 용균활성(사멸능)을 가진다. Erwinia amylovora (과수 화상병균)에만 특이적이고 다른 Erwinia 종(예: Erwinia pyrifoliae)에는 용균활성이 없어서 과수 화상병균에 대한 특이도가 매우 높으며, 같은 Erwinia amylovora 종내라면 모든 균주(strain)에 효과가 있기 때문에 균주-의존적이지 않다. 또한, 온도변화 또는 pH 변화에도 안정성이 높아서, 친환경적인 화상병 방제제로서 우수한 효과를 가진다.
도 1은 에르위니아 아밀로보라를 숙주로 하여 수행한 실험에서 본 발명의 박테리오파지가 플라크를 형성한 것이다.
도 2는 본 발명의 박테리오파지의 에르위니아 아밀로보라에 대한 용균활성을 확인한 것이다.
도 3은 본 발명의 박테리오파지를 투과전자현미경으로 확인한 것이다.
도 4는 온도에 따른 본 발명의 박테리오파지의 용균활성에 관한 것이다.
도 5는 pH에 따른 본 발명의 박테리오파지의 용균활성에 관한 것이다.
도 6은 유과에서의 과수 화상병 방제 효과를 확인한 것이다.
도 2는 본 발명의 박테리오파지의 에르위니아 아밀로보라에 대한 용균활성을 확인한 것이다.
도 3은 본 발명의 박테리오파지를 투과전자현미경으로 확인한 것이다.
도 4는 온도에 따른 본 발명의 박테리오파지의 용균활성에 관한 것이다.
도 5는 pH에 따른 본 발명의 박테리오파지의 용균활성에 관한 것이다.
도 6은 유과에서의 과수 화상병 방제 효과를 확인한 것이다.
이하 본 명세서에 대하여 더욱 상세히 설명한다. Erwinia amylovora(에르위니아 아밀로보라)에 특이적인 용균활성을 갖는, 기탁번호 KACC 97037P인 박테리오파지를 제공한다.
본 발명자들은 에르위니아 아밀로보라에 대한 용균 효과, 숙주 특이성이 우수한 신규 박테리오파지를 선별하여, 이를 Erwinia phage Fifi044(이하, Fifi044이라 명함)이라 명명하고, 국립농업과학원에 2021년 7월 27일자로 기탁하여 KACC 97037P를 부여받았다.
본 발명자들은 경기도 안성 서운면 배 과수원의 물로부터 박테리오파지 Fifi044를 분리하였고, Erwinia amylovora strain YKB14808을 숙주로 하여 수행한 실험에서 플라크를 형성하여 에르위니아 아밀로보라를 용해(lysis)시킴을 확인할 수 있었다(도 1).
도 2를 참고하면, 박테리오파지 Fifi044의 MOI (multiplicity of infection) 수치가 낮은 경우에도 숙주 세포의 생장을 억제할 수 있음을 알 수 있다(도 2의 control은 박테리오파지 Fifi044이 투입되지 않은 경우이다).
박테리오파지 Fifi044을 CsCl 밀도구배 초고속 원심분리법으로 순수 분리한 후 전자현미경으로 파지의 형태를 확인한 것을 도 3에 도시하였다. 약 65nm의 머리와 약 134 nm의 꼬리로 구성된 미오비리대 과(Myoviridae family)에 속함을 발견하였다.
본 발명의 박테리오파지 Fifi044는 에르위니아 아밀로보라(Erwinia amylovora) 종 내라면 모든 균주(strain)에 효과가 있다. 에르위니아 파이리폴리아(Erwinia pyrifoliae), 디케야 제아(Dickeya zeae), 엔티로박터 클로아카(Enterobacter cloacae), 대장균(Escherichia coli), 판토에아 아글로메란스(Pantoea agglomerans) 등에 대해서는 용균활성을 보이지 않는다.
또한, 본 발명의 박테리오파지는 식물 등 세균외의 다른 숙주는 감염시키지 않으므로, 항생제의 오남용으로 인한 항생제 내성균의 문제점, 식품 내의 항생제의 잔류 문제, 광범위한 숙주범위의 문제점을 해결할 수 있는 장점이 있다.
따라서, 본 발명의 박테리오파지는 에르위니아 아밀로보라 세균에 의해 유발되는 감염성 질환의 예방 또는 치료, 항생용 조성물, 사료 첨가용 조성물, 사료, 소독제, 또는 세척제 분야에서 다양하게 사용될 수 있다.
본 발명의 박테리오파지 Fifi044은 서열목록 1의 염기서열을 갖는다. 구체적으로, 박테리오파지 Fifi044는 53,2559 bp의 염기서열을 갖는다.
박테리오파지 Fifi044은 15℃ 내지 70℃, 20℃ 내지 70℃, 25℃ 내지 60℃, 25℃ 내지 55℃, 25℃ 내지 50℃에서 활성을 갖을 수 있다. 구체적으로 50℃에서 1시간 처리하여도 에르위니아 아밀로보라에 대해 용균활성을 보였다.
박테리오파지 Fifi044은 pH 1 내지 15, pH 2 내지 14, pH 3 내지 13, pH 4 내지 12, pH 4 내지 11에서 활성을 갖을 수 있다. 다양한 pH에 노출되어도 에르위니아 아밀로보라에 대한 용균활성을 보인다.
또 하나의 실시상태에 따르면, 본 발명은 상기 박테리오파지를 유효성분으로 포함하는 농약 조성물을 제공한다.
본 발명은 에르위니아 아밀로보라(Erwinia amylovora)의 종내 모든 균주(strain)에 대한 증식 억제 및 사멸 효과가 뛰어난 조성물을 제공할 수 있으며, 이러한 조성물은 박테리오파지 Fifi044을 유효성분으로 포함한다.
상기 농약 조성물의 박테리오파지는 과수 화상병(fire blight)의 예방 또는 치료에 사용된다.
상술한 본 발명에 따른 농약 조성물은 과수 화상병이 발생할 수 있는 과일들, 예를 들어, 사과, 배, 등에 이용될 수 있으나, 이에 한정되는 것은 아니며, 특히, 본 발명은 사과 또는 배의 장미과 식물의 과수 화상병 방제에 효과적이다.
한편, 본 발명의 농약 조성물, 과수 화상병 방제용 조성물, 에르위니아 아밀로보라(Erwinia amylovora) 증식 억제 또는 사멸용 조성물 중 선택되는 어느 하나는, 일종의 미생물 농약으로서, 액상, 분말상 등의 다양한 형태로 제조될 수 있다.
또한, 필요에 의해 미생물 농약에 사용되는 공지된 여러 부형제를 첨가할 수도 있는데, 상기 부형제는 미생물 농약에 사용되는 것을 특정의 것에 한정하지 않고 사용할 수 있으므로, 부형제에 관한 설명은 생략하기로 한다. 일례로, 본 본 발명에 따른 농약 조성물은 실제 포장에서 사용하기 적합한 안정적인 제제화를 목적으로 생화학 농약으로 사용가능한 계면활성제 또는 증량제 등을 부형제로 사용할 수 있다. 이 경우, 상기 농약 조성물은 수화제, 입상수화제, 액상수화제, 액제, 수용제, 수용성입제 및 캡슐화제로 이루어진 군에서 선택된 하나 이상의 형태로 제조될 수 있다.
상기 계면활성제의 일예로 알킬(C8~C12)아릴설포네이트, 디알킬(C3~C6)아릴설포네이트, 디알킬(C8~C12)설포숙시네이트, 리그닌설포네이트, 나프탈렌설포네이트축합물, 나프탈렌설포네이트포르말린축합물, 알킬(C8~C12)나프탈렌설포네이트포르말린축합물, 폴리옥시에틸렌알킬(C8~C12)페닐설포네이트와 같은 설포네이트 화합물의 나트륨염 또는 칼슘염, 알킬(C8~C12)설페이트, 알킬(C8~C12)아릴설페이트, 폴리옥시에틸렌알킬(C8~C12)설페이트, 폴리옥시에틸렌알킬(C8~C12)페닐설페이트와 같은 설페이트 화합물의 나트륨염 또는 칼슘염, 폴리옥시알킬렌숙시네이트와 같은 숙시네이트화합물의 나트륨염 또는 칼슘염, 나트륨 벤조에이트, 알킬카르복실레이트 등의 음이온성 계면활성제, 폴리옥시에틸렌알킬(C8~C12)에테르, 폴리옥시에틸렌알킬(C8~C12)페닐에테르, 폴리옥시에틸렌알킬(C8~C12)페닐폴리머, 에틸렌옥사이드 프로필렌옥사이드 코폴리머와 같은 비이온성 계면활성제, 폴리카복실레이트, 트리톤 100 및 트윈 80으로 이루어진 군으로부터 선택되는 하나 또는 둘 이상이 혼합되어 사용될 수 있으며, 이들 이외의 다른 계면활성제들도 사용될 수 있음은 당해 기술분야에서 통상의 지식을 갖은 자에게는 용이하게 이해될 수 있을 것이다.
증량제로는 벤토나이트(bentonite), 활석, 클레이, 카올린, 탄산칼슘, 규사, 경석, 규조토, 산성 백토, 제올라이트, 펄라이트, 화이트 카본, 암모늄 설페이트, 요소, 포도당, 덱스트린(dextrin), 콩가루, 쌀, 밀, 황토, 포도당 및 전분, 물 등이 단독으로 또는 2종 이상 혼합되어 사용될 수 있으며, 이들 이외의 다른 증량제들도 사용될 수 있음은 당해 기술분야에서 통상의 지식을 갖은 자에게는 용이하게 이해될 수 있을 것이다.
본 발명은 상기 박테리오파지를 유효성분으로 포함하는 항생용 조성물을 제공한다.
본 발명은 상기 박테리오파지를 유효성분으로 포함하는, 에르위니아 아밀로보라 (Erwinia amylovora)에 의해 유발되는 질환의 예방 또는 치료용 조성물을 제공한다.
상기 박테리오파지를 유효성분으로 포함하는 상기 항생용 조성물, 예방 또는 치료용 조성물은 기존의 항균물질 또는 항생제에 비하여 약물 내성 또는 저항성을 유도 하지 않아 안전하며, 에르위니아 아밀로보라에 대해 높은 특이성을 갖는다.
본 발명은 상기 박테리오파지를 유효성분으로 포함하는 소독제를 제공한다.
본 발명은 상기 박테리오파지를 유효성분으로 포함하는 세척제를 제공한다.
상기 박테리오파지를 유효성분으로 포함하는 상기 소독제 또는 세척제는 소독 또는 세척 효과가 뛰어나며, 에르위니아 아밀로보라에 대해 높은 특이성을 갖는다.
본 발명은 박테리오파지 Fifi044를 사용하여 과수 화상병을 방제하는 방법을 제공한다. 상기 과수 화상병은 에르위니아 아밀로보라 (Erwinia amylovora)에 의해 유발되는 것으로, 상기 박테리오파지를 유효성분으로 포함하는 농약 조성물을 박테리아에 처리하여, 에르위니아 아밀로보라를 사멸시킬 수 있다.
본 발명에 따른 박테리오파지를 이용한 방제(치료) 방법인 파지 테라피는 항생제 저항성 균주의 출현을 막고 환경에 영향을 끼치지 않아 안전하고 효과적인 과수화상병 방제를 기대할 수 있다.
본 발명은 상기 박테리오파지의, 과수 화상병(fire blight)의 예방 또는 치료에 사용하기 위한 용도를 제공한다.
본 발명은 상기 박테리오파지의, 과수 화상병(fire blight)의 예방 또는 치료용 농약 조성물의 제조에 사용하기 위한 용도를 제공한다.
상기 박테리오파지, 과수 화상병, 예방, 치료, 농약 조성물 등에 관한 구체적인 내용은 상기한 바와 같거나 이를 준용할 수 있다.
본 명세서에 사용된 용어는 특정의 실시예를 기술하기 위한 것일 뿐, 첨부하는 특허청구의 범위에 의해서만 한정되는 본 발명의 범위를 한정하려는 것은 아님을 이해하여야 한다. 본 명세서에 사용되는 모든 기술용어 및 과학용어는 다른 언급이 없는 한은 기술적으로 통상의 기술을 가진 자에게 일반적으로 이해되는 것과 동일한 의미를 가진다.
본 명세서에서 어떤 부분이 어떤 구성요소를 "포함" 한다고 할 때, 이는 특별히 반대되는 기재가 없는 한 다른 구성요소를 제외하는 것이 아니라 다른 구성 요소를 더 포함할 수 있는 것을 의미한다.
한편, 본 발명의 여러 가지 구현예 및 실시예들은 명확한 반대의 지적이 없는 한 그 외의 어떤 다른 실시예들과 결합될 수 있다. 특히 바람직하거나 유리하다고 지시하는 어떤 특징도 바람직하거나 유리하다고 지시한 그 외의 어떤 특징 및 특징들과 결합될 수 있다.
본 명세서에 있어서, "박테리오파지(bacteriophage)"란 특정한 타겟 박테리아를 감염시키는 바이러스이다. 단백 질 캡시드(capsid)로 싸여 있는 박테리오파지의 머리는 핵산 게놈으로 이루어져 있으며, 몇몇 박테리오파지는 지질 또는 단백질로 구성된 꼬리를 갖는다. 대부분의 박테리오파지는 유전 물질로서 이중나선 DNA를 포함한다
본 명세서에서의 "농약"은 농작물의 보호와 생장에 사용되는 약제를 의미하며, 농작물의 재배 또는 저장 중 발생하는 병충, 해충, 잡초 등을 방제하는데 사용하는 화학농약 및 생물농약, 농작물의 생리기능을 증진 또 는 억제하는데 사용되는 생장조정제, 약효를 증진시키는 보조제 등을 총칭한다.
본 명세서에 있어서, "방제"는 작물에 피해를 주는 각종 병해충을 예방하고 구제하는 것을 의미한다.
본 명세서에서의 "항생용 조성물"은 약제 형태로 동식물에게 제공되어 균을 사멸시킬 수 있는 제제를 의미하며, 방부제, 살균제, 항생제 및 항균제를 총칭하는 것이다.
본 명세서에서의 "항균제"는 미생물을 사멸시키거나 혹은 발육을 억제하는 물질을 의미하며, 방부제, 살균제 및 항생제를 총징하는 것이다.
본 명세서에서의 "소독제"는 병원 감염을 막기 위한 병원 및 보건용의 소독제로 유용하게 사용될 수 있고 일반 생활 소독제, 식품 및 조리 장소 및 설비의 소독제, 과수원 등의 건물, 축체, 음수, 깔짚, 난좌, 운반차량, 식기 등의 각종 생육 용품의 소독 등에 사용될 수 있다.
본 명세서에서의 "세척제"는 세정의 목적에 사용되는 약제를 의미한다.
이하, 본 명세서를 구체적으로 설명하기 위해 실시예 및 비교예 등을 들어 상세하게 설명하기로 한다. 그러나, 본 명세서에 따른 실시예 및 비교예는 여러 가지 다른 형태로 변형될 수 있으며, 본 명세서의 범위가 아래에서 상술하는 실시예 및 비교예에 한정되는 것으로 해석되지 않는다. 본 명세서의 실시예 및 비교예는 당업계에서 평균적인 지식을 가진 자에게 본 명세서를 보다 완전하게 설명하기 위해 제공되는 것이다.
[실시예 1] 박테리오파지 분리
1) 박테리오파지 분리를 위한 환경 시료 전처리
박테리오파지의 분리를 위해 과수 화상병 발생 과수원(경기도 안성 서운면 배 과수원)과 인근의 토양과 물을 채집하였다. 토양 시료의 경우 10 g, 물 시료의 경우에는 10 ml을 50 ml conical tube에 담고 TSB(tryptic soy broth) 10 ml을 첨가한다. 여기에 24시간 진탕 배양한 과수 화상병균(Erwinia amylovora) 94균주를 동일 비율로 섞은 혼합 배양액을 각각 1 ml 첨가하여 24시간 동안 진탕 배양하였다. 배양온도는 27℃이다. 배양된 시료는 10,000×g에서 5분간 원심분리하여 상층액만을 새로운 tube에 옮겨 담는다. 이렇게 얻어진 시료는 세균을 완전히 제거하기 위해 0.2 μm pore size filter로 여과하였다.
2) Dotting assay
박테리오파지의 증식을 유도를 위한 전치리를 마친 다양한 환경 시료에서 박테리오파지의 존재를 확인하기 위해 dotting assay를 수행하였다. TSB에 0.4% soft agar 첨가하여 멸균한 배지를 40~50℃로 준비하였다. 배지 3ml에 밤새 진탕 배양한 과수 화상병균 1종을 15 μl 넣고 기포가 생기지 않게 섞은 후 TSA(tryptic soy agar)에 얇게 부어 10분간 굳힌다. 균주가 균일하게 포함된 배지 위에 여과한 환경 시료를 10μl씩 떨어뜨인 후 27℃에서 정치 배양한다. 여러 개의 환경 시료를 하나의 배지에 함께 떨어뜨릴 경우 시료가 서로 섞이지 않도록 충분한 간격을 유지하여야 한다. 24시간 배양 후 시료 부분에 세균이 자라지 못하고 투명하거나 플라크가 확인되면 양성으로 판정하였다.
3) Plaque assay(overlay assay)
박테리오파지를 순수분리하기 위해 overlay assay를 수행하였다. TSB에 0.4% soft agar 첨가하여 멸균한 배지를 40~50℃로 준비한다. 배지 3 ml에 밤새 진탕 배양한 과수 화상병균 15 μl와 박테리오파지가 확인된 양성 시료를 10μl 넣고 기포가 생기지 않게 섞은 후 TSA에 얇게 부어 10분간 굳힌 후 27℃에서 24시간 동안 정치 배양한다. 이때 양성 시료는 단일 플라크를 얻기 위해 십진희석하여 농도별로 수행해야 한다. 24시간 배양 후 크기나 모양이 다른 플라크(plaque)를 선별하여 1 ml의 saline-magnesium(SM) buffer에 넣어 냉장 보관하였다. 각 플라크에서 추출한 박테리오파지는 단일 박테리오파지 분리를 위해 동일한 과정을 5번 이상 반복하였다. 이때 형성된 플라크(plaque)를 도 1에 나타내었다.
[실시예 2] 용균활성 확인
과수 화상병균 (Erwinia amylovora YKB14808)을 TSB 배지에 전날 진탕 배양하여 준비한 뒤 24시간 후 재배양한다. 이후 세균의 농도가 OD600nm=0.5(108 CFU/ml)에 도달하게 되면 TSB 배지에 십진 희석하여 105 CFU/ml로 접종농도를 맞춰준다. 세균 배양액을 96-well plate에 180 μl씩 분주한 뒤 파지는 multiplicity of infection(MOI) 0.001, 0.1, 10, 1000에 맞춰 20 μl씩 접종한다. 이후 24시간 동안 마이크로플레이트 리더기에서 27℃, 120 rpm으로 진탕 배양하며 1시간 단위로 세균의 OD값을 측정한 것을 도 2에 나타내었다..
[실시예 3] 형태학적 분석
박테리오파지의 증식을 위해 TSB 3 ml에 화상병균 (Erwinia amylovora YKB14808)을 24시간 진탕 배양한다. 24시간 진탕 배양한 화상병균 2 ml을 TSB 200 ml에 재배양한 후 OD600=0.5가 되면 108 PFU/ml 농도의 파지를 1 ml 처리한 후 24시간 진탕 배양한다. 24시간 진탕 배양한 시료의 불순물을 제거하기 위해 10,000×g에서 10분간 원심분리하여 상층액을 0.22 μm pore size filter로 여과하였다. 박테리오파지 시료에 Ployethylene glycol 8000(PEG 8000) 5g, 5 M NaCl 10ml을 처리한 후 4℃에서 24시간 배양한다. 24시간 배양한 시료를 9,000g에서 20분간 원심분리한 후 상층액을 제거하고 침전물을 SM buffer로 현탁하여 준비한다. 초고속원심분리용 튜브에 CsCl 밀도층(1.3 g/ml, 1.45 g/ml, 1.5 g/ml, 1.7 g/ml)을 역순으로 3 ml씩 쌓은 후 그 위에 농축된 파지 시료를 쌓고 785,500×g에서 2시간 동안 원심분리하여 정제된 파지를 얻었다. 정제된 파지를 SM buffer로 투석한 후 0.22 μm pore size filter로 여과하였다. 여과된 파지 시료를 2% uranyl acetate 용액으로 염색한 후 투과전자현미경을 통해 형태를 확인하여 도 3에 나타내었다.
[실시예 4] 기주범위 분석
분리한 박테리오파지의 기주범위 분석은 Erwinia amylovora 94 균주, Erwinia pyrifoliae 25균주와 Bacillus thuringiensis, Dickeya zeae, Enterococcus faecalis, Escherichia coli, Pantoea agglomerans, Pectobacterium carotovorum, Pseudomonas fluorescens, Staphylococcus xylosus, Xanthomonas campestris를 사용하였다. 양성 대조군으로는 화상병균 방제용 박테리오파지 제제인 AgriPhage을, 음성 대조군으로는 SM buffer를 사용하였다. 기주범위 분석에는 dotting assay 방법을 이용하였으며 24시간 배양 후 플라크가 확인되면 양성으로 판정하였다. 구체적으로는 투명환이 생성되는 경우를 +로, 그렇지 않은 경우를 -로 기재하여, 하기 표 1에 기재하였다. 모든 실험은 3번 내지 9번 반복으로 수행하고 관찰된 플라크의 단계점수의 평균값을 기록하였다.
| No. | 타겟 박테리아 | 스트레인 | 박테리오파지 Fifi044의 용균활성 |
| 1 | Erwinia amylovora | YKB 14715 | + |
| 2 | Erwinia amylovora | YKB 14740 | + |
| 3 | Erwinia amylovora | YKB 14742 | + |
| 4 | Erwinia amylovora | YKB 14748 | + |
| 5 | Erwinia amylovora | YKB 14750 | + |
| 6 | Erwinia amylovora | YKB 14754 | + |
| 7 | Erwinia amylovora | YKB 14756 | + |
| 8 | Erwinia amylovora | YKB 14758 | + |
| 9 | Erwinia amylovora | YKB 14768 | + |
| 10 | Erwinia amylovora | YKB 14770 | + |
| 11 | Erwinia amylovora | YKB 14776 | + |
| 12 | Erwinia amylovora | YKB 14778 | + |
| 13 | Erwinia amylovora | YKB 14787 | + |
| 14 | Erwinia amylovora | YKB 14806 | + |
| 15 | Erwinia amylovora | YKB 14808 | + |
| 16 | Erwinia amylovora | YKB 14814 | + |
| 17 | Erwinia amylovora | YKB 14818 | + |
| 18 | Erwinia amylovora | YKB 14820 | + |
| 19 | Erwinia amylovora | YKB 14822 | + |
| 20 | Erwinia amylovora | TS3128 | + |
| 21 | Erwinia amylovora | RA0023 | + |
| 22 | Erwinia amylovora | RA0024 | + |
| 23 | Erwinia amylovora | RA0025 | + |
| 24 | Erwinia amylovora | RA0026 | + |
| 25 | Erwinia amylovora | RA0027 | + |
| 26 | Erwinia amylovora | RA0028 | + |
| 27 | Erwinia amylovora | RA0029 | + |
| 28 | Erwinia amylovora | RA0030 | + |
| 29 | Erwinia amylovora | RA0031 | + |
| 30 | Erwinia amylovora | RA0032 | + |
| 31 | Erwinia amylovora | RA0033 | + |
| 32 | Erwinia amylovora | RA0034 | + |
| 33 | Erwinia amylovora | RA0035 | + |
| 34 | Erwinia amylovora | RA0036 | + |
| 35 | Erwinia amylovora | RA0037 | + |
| 36 | Erwinia amylovora | RA0038 | + |
| 37 | Erwinia amylovora | RA0039 | + |
| 38 | Erwinia amylovora | RA0040 | + |
| 39 | Erwinia amylovora | RA0041 | + |
| 40 | Erwinia amylovora | RA0042 | + |
| 41 | Erwinia amylovora | RA0043 | + |
| 42 | Erwinia amylovora | RA0044 | + |
| 43 | Erwinia amylovora | RA0045 | + |
| 44 | Erwinia amylovora | RA0046 | + |
| 45 | Erwinia amylovora | RA0047 | + |
| 46 | Erwinia amylovora | RA0048 | + |
| 47 | Erwinia amylovora | RA0049 | + |
| 48 | Erwinia amylovora | RA0050 | + |
| 49 | Erwinia amylovora | RA0051 | + |
| 50 | Erwinia amylovora | RA0052 | + |
| 51 | Erwinia amylovora | RA0053 | + |
| 52 | Erwinia amylovora | RA0054 | + |
| 53 | Erwinia amylovora | RA0055 | + |
| 54 | Erwinia amylovora | RA0056 | + |
| 55 | Erwinia amylovora | RA0057 | + |
| 56 | Erwinia amylovora | RA0058 | + |
| 57 | Erwinia amylovora | RA0059 | + |
| 58 | Erwinia amylovora | RA0060 | + |
| 59 | Erwinia amylovora | RA0061 | + |
| 60 | Erwinia amylovora | RA0062 | + |
| 61 | Erwinia amylovora | RA0063 | + |
| 62 | Erwinia amylovora | RA0064 | + |
| 63 | Erwinia amylovora | RA0065 | + |
| 64 | Erwinia amylovora | RA0066 | + |
| 65 | Erwinia amylovora | RA0067 | + |
| 66 | Erwinia amylovora | RA0068 | + |
| 67 | Erwinia amylovora | RA0069 | + |
| 68 | Erwinia amylovora | RA0070 | + |
| 69 | Erwinia amylovora | RA0071 | + |
| 70 | Erwinia amylovora | RA0072 | + |
| 71 | Erwinia amylovora | RA0073 | + |
| 72 | Erwinia amylovora | RA0074 | + |
| 73 | Erwinia amylovora | RA0075 | + |
| 74 | Erwinia amylovora | RA0076 | + |
| 75 | Erwinia amylovora | RA0077 | + |
| 76 | Erwinia amylovora | RA0078 | + |
| 77 | Erwinia amylovora | RA0079 | + |
| 78 | Erwinia amylovora | RA0080 | + |
| 79 | Erwinia amylovora | RA0081 | + |
| 80 | Erwinia amylovora | RA0082 | + |
| 81 | Erwinia amylovora | RA0083 | + |
| 82 | Erwinia amylovora | RA0084 | + |
| 83 | Erwinia amylovora | RA0085 | + |
| 84 | Erwinia amylovora | RA0086 | + |
| 85 | Erwinia amylovora | RA0087 | + |
| 86 | Erwinia amylovora | RA0088 | + |
| 87 | Erwinia amylovora | RA0089 | + |
| 88 | Erwinia amylovora | RA0090 | + |
| 89 | Erwinia amylovora | RA0091 | + |
| 90 | Erwinia amylovora | RA0092 | + |
| 91 | Erwinia amylovora | RA0093 | + |
| 92 | Erwinia amylovora | RA0094 | + |
| 93 | Erwinia amylovora | RA0095 | + |
| 94 | Erwinia amylovora | RA0096 | + |
| 95 | Erwinia pyrifoliae | Ep12327 | - |
| 96 | Erwinia pyrifoliae | RP0098 | - |
| 97 | Erwinia pyrifoliae | RP0099 | - |
| 98 | Erwinia pyrifoliae | RP0100 | - |
| 99 | Erwinia pyrifoliae | RP0101 | - |
| 100 | Erwinia pyrifoliae | RP0102 | - |
| 101 | Erwinia pyrifoliae | RP0103 | - |
| 102 | Erwinia pyrifoliae | RP0104 | - |
| 103 | Erwinia pyrifoliae | RP0105 | - |
| 104 | Erwinia pyrifoliae | RP0106 | - |
| 105 | Erwinia pyrifoliae | RP0107 | - |
| 106 | Erwinia pyrifoliae | RP0108 | - |
| 107 | Erwinia pyrifoliae | RP0109 | - |
| 108 | Erwinia pyrifoliae | RP0110 | - |
| 109 | Erwinia pyrifoliae | RP0111 | - |
| 110 | Erwinia pyrifoliae | RP0112 | - |
| 111 | Erwinia pyrifoliae | RP0113 | - |
| 112 | Erwinia pyrifoliae | RP0114 | - |
| 113 | Erwinia pyrifoliae | RP0115 | - |
| 114 | Erwinia pyrifoliae | RP0116 | - |
| 115 | Erwinia pyrifoliae | RP0117 | - |
| 116 | Erwinia pyrifoliae | RP0118 | - |
| 117 | Erwinia pyrifoliae | RP0119 | - |
| 118 | Erwinia pyrifoliae | RP0120 | - |
| 119 | Erwinia pyrifoliae | RP0121 | - |
| 120 | Dickeya zeae | - | |
| 121 | Enterobacter cloacce | - | |
| 123 | Escherichia coli | - | |
| 124 | Pantoea agglomerans | - | |
| 125 | Pectobacterium carotovorum | - | |
| 126 | Pseudomonas fluoresceus | - | |
| 127 | Xanthomonas campestris | - | |
| 128 | Bacillus thuringieusis | - | |
| 129 | Clavibacter michiganensis | - | |
| 130 | Enterococcus faecalis | - |
상기 표 1을 보면, 박테리오파지 Fifi044은 높은 화상병균(Erwinia amylovora) 특이성을 가지고, Erwinia amylovora 종내라면 모든 균주(strain)에 효과가 있기 때문에 균주-의존적이지 않는 것을 알 수 있다.
[실시예 5] 온도 및 pH 안정성
박테리오파지들의 다양한 온도와 pH에서의 활성 유지 여부를 확인하기 위해 안정성을 분석하였다. 각 파지의 농도는 105 PFU/ml을 사용하였다. 각 파지는 1.5 ml tube에 200 μl씩 분주한 후 30℃, 40℃, 50℃, 60℃에서 1시간 동안 처리하여 dotting assay를 통해 활성 농도의 변화를 확인하여 도 4에 나타내었다.
pH는 SM buffer에 HCl, NaOH를 이용하여 pH4-11에 맞게 제조하였다. 각 pH에 맞게 제조된 SM buffer 990 μl에 107 PFU/ml 농도의 파지 10 μl을 1시간 동안 처리한 후 dotting assay를 통해 활성 농도의 변화를 확인하여 도 5에 나타내었다.
본 발명의 박테리오파지 Fifi044은 온도변화 또는 pH 변화에도 안정성이 높아 친환경적인 화상병 방제제로서 우수한 효과를 가짐을 알 수 있다.
[실시예 6] 염기서열 분석
박테리오파지 입자에 단백질 분해효소를 처리하여 외피를 제거하고 Norgen사의 phage DNA isolation kit를 사용하여 각 파지의 genomic DNA (gDNA)를 추출하였다. 100~200 ng의 gDNA를 TruSeq Nano DNA kit를 사용하여 library를 제작 후, Illumina HiSeqXTen 플랫폼을 기반으로 염기서열 분석(next generation sequencing, NGS)을 수행하였다. 얻어진 전체 read는 단일 contig를 얻기위하여 assembly를 진행하였다. 서열분석으로 예측된 Open reading frame 각각의 기능을 Basic Local Alignment Search Tool (BLAST)와 HHpred을 이용하여 분석하였다. Erwinia phage Fifi044의 genome은 53,559bp의 크기를 가지며 78개의 open reading frame (ORFs)이 예측되었다.
[실시예 7] 과수 화상병 발생 억제 효과 확인
과수 화상병균 (Erwinia amylovora YKB14808)을 TSB배지에 전날 진탕 배양하여 준비한다. 세균 배양액을 TSB 배지에 재배양하여 OD600nm=0.5 (108 CFU/ml)에 도달하면 1,800×g, 5분간 원심분리로 화상병균을 침전시키고 이후 Saline solution으로 씻어낸 뒤 10배 희석하여 세균 현탁액을 준비한다. 실험에 사용하는 미성숙과는 70% 에탄올로 표면 살균한다. 멸균된 1 mL 팁을 이용하여 미성숙과 표면에 구멍을 2개 뚫어준다. 미성숙과 표면에 만들어낸 구멍에 1010 PFU/ml의 파지 용액을 10 μl를 흐르지 않게 접종한다. 이후 파지 용액이 완전히 마르기 전에 107 CFU/ml의 세균 현탁액을 10 μl를 접종한 뒤 상대습도를 90% 이상으로 유지하고 27℃에서 약 2주간 2일 간격으로 병 발생 정도를 확인한 것을 도 6에 나타내었다. 도 6을 보면, 박테리오파지 Fifi044이 접종된 유과는 과수 화상병이 방제됨을 알 수 있다.
[염기서열]
서열번호 1
TGTTCCATGTGAGTTACTGAGTTCCGTAATCCTATTATAAAGGCCCTAACACAAGCCGGG
ATTGAATTGGAACTTGCTGGCTCAAATGGAATAGTGGTGACAAAATCGAGAAGTGAGTGG
CATTCGGGTTCAGTGGCGTTTCGTTGCGTTAACTAAGCCCGAAGCCGACATAAAATTAGT
AACAAAATTTGTGGCAGTATGGCATGTTAATATCGTTTCAATATTGCGATTACTTCTGCA
TGTTAATATGTGACCGATTGTTAGAGGACTAATCACCTCGAAGATACGATTGACTGGTCA
AATTGGCATGTGACTCGTTTGCAATATTGTGACAAATAGATGCATGTAATATTGCCACAG
TTTTCTGCATGTAATACTGTAACCCGCATGGTTACTGGGTTTGGCTAAACCTGTTAGCAT
AATCACAGGTACTATATTTGTGACAATATTGGGAATTAAAAGATTTATTCTTTTTCTTTC
TTTTGTTGGCTGGTCACTCAACTGTACCAGATTTCTGAACTTTGAATGTGCCAAATACAT
TTGAGTTTCGAGTCTGGGATAGTTACAAGACCCGCATGTGTAATGTGACAATTAAGTGAG
ATTTGGCCTCACAGTATTGTTCCACATTAGTTGCATGTGAATGAAAGACAAAGAGTTTAT
CTCTCAATTTATTCGGTGATTTTGGCTGAGTAAGTTGCGAGATAGGCTCCAAACTATCAT
TAGGATGTACATCGGGCACTATGCTAGTCACATGGTGCCCTTCTTAATTTACTTTGGAGA
TACAAAATTATGGCTCGTTTCGGTGATTTGAAAGATGGTCAACAGTTTGCCGTTGGTGAA
ACTGTGTTCTGGAAAGTTTTGGAAGAATCTGAATTCTATAATGCTGTCGAATGCAAAGAT
AGCAGCAAAGCAATCATGATTCTTGACGATTGTGAAGTAGAATTGGAGCACGAATAATGG
CAATTCTGTTTATCAAATATCGTGACCGTGCCAACGAAGTTCAAATCGAAGGTACGTTGT
CTGAATTGAAAGTTCATCTGAAAGGTTTGGATGAAACTGTTGTTGAATATGTACTGTTGG
AAGATGTTGATGGTACAACTGGTGATGATATTGAATCACTGATTACATACTGTGAATTTT
GGGAGACGATTGAATGAGTCGTTTCGATTGTAAGAACAAATTGTATCGAACTCCCGGAGG
ACGTTTGATTCTCCGGCATTGGATTGAGCAACGTCACGGTTGGGATTTCTATATGAGAGA
TTATGATGATAACCGGTGGTATCGTGCTGGTAATCAACTTTACTGTGAAGATGTGTGCAA
CTATCAAGCCAAGCTCATTGGCATCAACTTGTCAAACACAGGTCTATAATGAAAACTGGT
GAACGTGTTGTGATGAAGCATGTTGAAGCTCGTCTCGACGGGTTCACTGCTTACAAAAGT
TATGAAGTTGTCTCAGGTGAAGGTGAAGTTAATGTGAGTCCAATTGCTTTAAAGTTGGGC
ACAATGATTCACCACAGTGAGAGAACTTTCAATCTAATTGATGATAAAGGTAGGACACGT
TTTTGTTCTACAGCTCATTTTCGTCCGTTTAATGCAGAATTGGGAGGATTATTCCAGTGA
TTCAATTACAAGGTCAACCGCTCCAAGATTTCCTCGCATTTCTGTCCGACGCATATCCTG
AAATAAATGGTGCCGAATTCGTAAATAATGGCATCATGAATATCTCAACTGATTCAATGT
TGTACAAAGCATGGGAAGAATTCGATGAATACTGTGGATGATGAAAGCGCAGCCTTTCAC
AACTCTGGCTTACCTGTAGAGGTATAAAAATGTCAAGTCTGATTGCGTTTAATAACGTAG
TTCGTCGTGCTCAAACTAAAGCCGACAATGCAGTAAGTTTGATTGAGAATGTTCCAATCA
CAACTAATGCAGCAATTATTCTGTTTGCGGATAAACACATTCGCATGATGGAAGAGCAAC
AAGCAGCAATGCAGCGTGAATTAACAGAACTTCGTGCATTGGTGGCTGTATCAAGATAAT
TTTCATAAACTGGATGTGGGTTATGGCGATATTGTTTCTGAGCCAGAACAAATCGCAACT
CGTCAGGCATCAAACCGTTAAAGTCCTGACTCCAGTTACACCAAGCGAATCCCGGCTTTG
GGCTTATGCCCTGTTTCACCATTCGCGAAGGTTTACATATCAAGTTTGAGTTGACAACAA
TGAAACTCCCGATGGCTCAATAATGCGGTTCGAGCGCAAAAAGGCTGGATACTCCAGCTC
ATAAACGTAAGTAGCTTTTGTATTCGTTCTCACTACTTCATAGCAGGATTAAGTTGCCGG
TGACTATAAGCAGTCTGAACAAATACCAAATGTTATTTAAATGTCGGCAAGATGCAGCCA
AACTATCATTAACTTCATAGGAGCAGTAAATGGCGATTCTACGCAGAAACAAACTCGGTC
AAATGACCAAAGCCCGCGTTCGTGTTGGCGACATTGCAACGATTTCAAAATCTTCAACTG
TTCTCGGATTAACCGCGAATAAAGGATATAAAGTCTATGCAACGTCACTATCGAGCGAGG
ACAAGCGTTCTTATATTGGCGCTCATTATCAGTGGGCTTGTGTTACTGAAAATCACTTTG
TCGTAATCAACGACCGTGGTGAGAAAACCTTCTGTTCTTTGACTCCAAAACATCCAAACT
ATGATGTTGCAGCGAGCAAGGCAAACTTTGGCGAATGGACGGTGTAATATGTCATATTAC
GTTGATGAATTGGGTGTAGTTCATTTTGTGAATCCTCGCAAATGAAGTACGCAAATGCTG
TTAAGAACGAGGACGGAAGTTTAAAGAATTTCGTCCTGACAGTTGCTGGAAAAGACTTTA
ACTGTTCTTGTGGTTGCAATTGCTTCCACAAGCCAGAAAAAGAGAATCCAAAAATCTATC
GCTGCAATGCTTGCGGCACAACTTATGGAAGTCGAGAACCGAAATGAACGAATTTCTGAA
TTATTCTATCCTCGGCATTATGACGGTACAAACGTATCTGATTATTGCTGCAATTGATTT
ATTCTTGCAATGCTGGTTGAGCAAGAAACATCGTCATTTCAGTAATCGTGTTGCACGAGC
GCTGGCATGGCCAATTTTGATTGTTGTACTCATCATCATTTTCGTTCTGGCATCATTTGT
TGGCCTGTATGAAATCATGTCGAAGGCGAAGCGCAAATGAACAAAGGTGATTTAGTTGAA
TGCTGTGAATCAATCGGCAAAGCTTTCATCAACAAAGCCAGCATCAAAGAAAACGCGTTC
ACTAAAGGGCGTGTTTATGAAGTTCTTGCTGGTTACGGTGACGGTGATATCAGTCGCAGT
GATGGTCGAATTGGTGCGTTTATCATTAACGATTACCAAATGTGTGTTAAAGACGACTTT
GGCGATATCCGCTTAATTAAGTGTGACCATCGTTTCAATGTTGTGCATTTGAGTCCAACT
GCGAATAAGCGCCGTGTTCCGGTGACTCATTTGTATCATCCAATTCCAGTGGGCCGATAG
GCCAGGAGCAACTTTATGTGGTATGGTTCTTCATTCAAAAATGGTTATGGTGGCGAATCA
TTTACTTTCCGTAAAGCTCGCAAATCCCGTAACGGTGGCTTGGGCTGGTGGATTACAATT
GCTGTAATCTTCTGGCTCTTCTTTGGTTCACATATTCCGACAATTTTCTGATATGAGAAC
ATATATAAACAGATGGAAGCAAGATAGAAAGAGGCATCTCATGTCTATGTTGTCTGAATA
TCAAAGTGTGAAACAATCTTTGATACACGGACTGCATGGTCGTGACAGATATCTGGAGAA
ATCTAGATTTAATGTAAATGATTACTCTTTCTATGCTCCACTCATTCGTCAAGTACAAAA
GAAAATTAATCTGTATACAAAGCGCATTCATTGAGTGCGTTTTAATATTCACGATTAGTT
GGATGTGAAGAACAGCAATGACGCTGTAACTTAAACTGTAAATTACTTAAATAAAGAGGC
TTTATCATGTCTAATACCAAAACTTACGACGATACAAACACTGGCATCATCGGCGCAAAC
GACTACAAACAGAAGGATACTCATCCTGACCAACGTGGTCGTTGTAATGTAGGTGGCCTG
TGGTATTGGGTTTCAGGTTGGGATAAGAAATCCAACAACCGCACTTTCACATCTTTGGCA
TTCACTCTGATGACTCAGGATGAAGTTGACAAAATGATGGCGAAACGTGCAGAGAAAGAA
CGTCCTCAGCAGGGTGCTCAACAGCAGCAACCACAGCAACAGGCTCCGGCTCAATCTCAG
CCACAGCAGCAAGCTCCAGCTCAGCAAACTCCACCAGCGACTCAGAACAACGAACCGCCA
ATGGATTTTGATGACGACATTCCCTTTTGATTTGCAGCGAGTTCTTAAACAAGAGCGCAG
GATGCTGCGAGAAATCATAGGTAACTAATGAGCCAATTCCTCGAAACTGTGTACGCCAGT
GAATTACGTGCTGGCGACATTATTTATTACGAGGGTGATTTGATGCAGGTAGAAACAAAC
TACCTGCATCGCAATTCACCATATGCGAAACTTAATTTAGTTCCGGCAGAGAATCCAAAA
CATATTCCACTGTCTGTTAAATTAGGTTTGTTCACGAACATTCAGAGAAGAACTTACGTG
CACACAAAACATCTGCATATTAGCAATTCAATTGAGATTATGTCTGAGTTGAACGTGCTG
CTTGAGCAGTATGACAATGCGAACGAATTACCTGAAAAAGTGATGTTGGCAAACACCAAT
TTCGAAGTTGATTCGGTGATTGTTCAGCAAATGGTGCACGAGAAAATGCGTGCTTGTTTC
AATCGTTTGAAAAACGAAATTGAAATGATTAAGAAACCAAGTTAATTTTGAGAGGTTGTT
CCGATAGGAGTGACCTCAATTAAGTTAATTTATTCAACAAAGCAATAAGGTAACAAATGG
ATAAGGAAGAGGTTCAAACCTTTGACGCAACATACGATACTCTGGTTCAAATTCAGACAA
AGCAATCAAAGATTTCTTCATTGATTGTTGAATTCGACCAAGAATATCACGGTGAAGCAG
ACCCAGCCAAACTTAACTCTATCAAGGTTAAGATGGCAACACTCAGCAAAGAAATTGGTG
AACACATCAATTCTCTGTGAGTTGCAGTTAAATCTTACAGGTTCTTATCTCCGATAGGAG
CCTGAATAGGTTCAATTGGAGGTCCTATGTACGTCTACTACCAAAATTTCATTTATTGTA
GTGATTGGCCTAACCATTACAATCGGTCCATGTACACGCATCAAATTACTGTTCACGAGA
ATCAATCTCCTGAGCAGTATGAAGCATTGCGAATTAAGTTCCACCAGCATAAAGTTTCGT
ACAGTAAGTGGACAAAGAAAGTATACATTCGTGTCTTAGCCAGACGTGTGAAGGTGAATA
AAACTCACAAATCTCATCCTTTCTATGGAAAGATGACAACAACCCATTGGGCAGAAGTTC
GTAACTATCTGCTAAACATTAATTACAGGATTGATTACGATGAAGATGGTACTAAAATTA
TCAGCAATGTTCTGGATTAGTTTCGCGATTTTAGTTGTTTATGGTTGTTCGCATCCAGTC
CATGCATCAATGCATCAAGTCACCTTTGAGTGCAATGGTGAGCGTCTCATCTTTACCCAA
CAAGACAAGAATCCCATCTTGTTGGAGGTTGTTCGGACAAAAGGAATCTGGATGCAGGTT
CCTTCGGACAAGCCAGTCCAAATCTTTTACAATCTCGACTCTGGTAAAGTTGCCAAAGTC
GGGAAGAATTATTCAGGTGCATACTATGTCGAATTTTATAAAGACACAGATTATGCAAAC
GCATTTAAACCAGAGAGAACATTGCCGTGCCGGACAAAATAGACCTCGAATACCAATATC
GCCGTCTAACTGCCATCATTGATAAAGTCAAGAATGGACATTTGGTGAAAGGCAAAGTTG
TTGCCGATGCTGGAGAAGGATTAGCAGTTGATTTGGATTTAACATCCGATGAGCTGTTGG
CAATTCTCCACCAGCGTGCAGGCAAAGTTTTCAATCAGTGGAGAAACGCTAAAGTTGAAA
GGTATTCACAGCCAGTTCAAAATTGACGGAACCGACTATCTTATCGAGTCCGAGTGCTCT
CAAGCAGTGTTCCCACGACTTACCGATAAGGTAATCCGTCATTTGGAGCGCATCGGTAAA
CATGCCGCTGCGGAAATGTATGGCCATGAAGCTGTTGTTTCAAGCTATACGAAAATCAAT
AACGAGTTAGTGAAATTCAAACTAACCGTAAATCCAAAAGGATTACCATGCAGTTAAACG
AATTAGGCGCAGCTGTTCAGGCTGCCGCTGATGCCGCTGCTGACCAGAAATCTTTTGTCG
ATAAACTGACTGAGATGTATGACTCAGGCACAAAAACTCTTAGTGTCTTTGTTGAAGAAA
CTCAGTCACGGACAGAAGTTGACCTCGCATCTTTCTGGCCGTTGCAAAATGACAAACTGG
TCAAATTGATGCAATACCAAGGCAATCTATCCGGTATTCAAACAAATTTGCAGCAAGCGC
TGGACCAGGCAATTGATGGATTAACAAATCCACCAGCTGCAAACGCAACACCGGTTGGAG
AAGTTTCGGCATGAGTAACGTCAAACTGAGTCCATCAAGTCAGGCAAAGCTGAACCAAGT
CCGTATGGATTTGCGTCAGGTTTGTGCGAAGGCATTTGAAACCTTGCCTTTTGATGTGAC
TGTGATTGAAGGTTTACGCAGTCAGAAACGTCAGGCTGAATTGTTTGCACAGGGTGCAAC
CAAAGTTATGACGTCTCGGCATATGTCCGGCAATGCTGTTGACTTGGCTCCATATCCAAT
CGACTGGAATGATACCGAACGTTTCCGCATCCTCGCGCATCACATGTTTGCAGCGGCGAA
GGAGCTTGGCATCACCATTCGTTGGGGCGGTAATTGGTCGCGGATTGATGAAAATCAAAA
GCCACCATCAAGCTTTGTTGACATGCCTCATTTCGAGTTGCCAGCATGAGTACTCAAACG
GATACAGAAGTTTTCCTTCTGACCGAAGAGTATAAAAGGTTGGAAGAAGAGCGTGCCGAA
CACCAAACCAACTTTGATTTGATTGTTGAATCGTTCAAAGAACCGGTTCCCGGTGGTGTT
GTTACGGATGACTCAGTTGCAGAAATAATTCTGATGAACCCAGGCCGTGTCAAACAGTTC
AAAGTTTACACTCAGTTTCTTCTTGCAGGTTTAAAACACCGCATGGATGAAATTGATGCA
CGCAAAGTGAAGCTGGATGAGTTAATCATTTATGCAAACGATGCAGTAGACTACCCACCA
GCATAATCCATAGCGGAGAATCCAATGGACTATAAAGATTTGCCAGACCGCATCAAAGAG
TTGAAGCGTCACAACGAAGACCGCAATCGTGCAGTGCGTGAGTGCGATAGACCAACCAGT
AAAGAATGGATGGTTGTCCAAATTGAAGGCGACTATGTTCGTGTTAGTGCGGAACATTTA
AAGGCAGCAATCGCCAAAACAACCGAAGAAGTTGATGTTGAGTTATCCAAACTCACAGAC
GTTCACGATACGTTGAAAAAGGTTGCCAGCGGCTTGCTGTAATAGGCCAGCAGTGAAAGA
AATAATGCAACGGCGTCCTTACATGGACGTCTTAAATTCGGAGCAAGTTATGGACGGGTA
TAGTTACATTCGAAGAATTGAATCGACAAACCATGTTCGTGATATTTGTGTTCGATTTTA
TGGTTTGAAGTCAGGAACTGATATTTGGTTAGATGCACAAGCATTGTATCAAGCCAATCC
ATTGGGTTCTGTAAGTATGGCAACCTTCCTCAGAACTCATTGTGAGATTGAAGAAGATGT
TGCTGAACGAATTGCAAATGCAGATTGGGAAGTTGTTGCAGCAGAAGGTTTGTGCGAACG
TTGCCTGACTCAGCCGAAAGGCTTTGATTGGGAAATGTACGAAGAAATCAGTGACGCTCG
TAGCGATTATGCCGACGAAATTGTTTTAGCGGCGATAGCCTGCGATGTTCCATTATCCCT
TATTCAAGACCGCTACATTGGCGAGTTCAAGGATTGGGAATCATTTGTCAAAGAACGTTG
GGACGAACTCTACAAAGACGAAGTTCCAGAATTCCTGGAGAATTACATTGACTATGACAA
AATCACAGTTGATTGGAAAGCAGGTGGAGAATACTCCGAAGACAACAATCATTTCTTCGC
AACAAGCTGAGCCAACAAAGTGGACTGGTGGTGAAATCATTTATCACCAAGACCGTCAGC
ACTATTACCAAGTCATCAACCGTTGTTCAGGCAAAGGCCCGAATGGATGGGTTGAAGGCT
GGACATATGTTCGCGTGAAGGATTTGTTTGGCAATCAAACAGCGTTTGAAGTTGATTGTG
ACCCGGACAAGTTCAAGATGTACTTCCGACCAGACGAATATTTTGATTCTGACTGGATTT
TAATTCGTGCGGTAATTTTGTAGCAGTGTGAGCGCTTCGCGCTTTCGGCATTAGCTGGAG
GTGCGAAGCACATTGTAATCACTTGGAACATTGGTGTTCCAGCTTGGCACGTTGGTGTGT
TCCCACAAACAACTAAAGTGTGGAACGTGTGCTCTGTAGGTCAAGGACATTATACAAAAT
TGTGTCAAGGGTTAATAAACACATTGTGACGATAGATGCAGCCACATCTACCGGAAAAAG
TGGTTATGACCAAAACCGATAATAACGGCGTGAAACTATCGTTAAAACAGGTTAGTCACA
AAGTCTGTTTTGAGATAGCGTTATGCTATCATTCACTTGCACTATTAACTTTTAACAATA
AACCGTGAGAATACGAAATTATTCCCGCTATGTTGGGTTGGTGCGGCGAAGTTGTAACTA
CAATATACCGGCGAGACATATTTCAACCTGCCTTGATGATTGTGAGCCATCATAATTATC
TTTAAGGGCACATAATGAAACTACATGTGGGTAAACTCGACCGGGTGAATGTGACTATTT
GTACCAAGCTGTTAGGGCTGATAATTTTGGGTAAAACCAGTGGTATAGATAGCGATTCTG
CGACGGCAGCGGCCTTGTGCCGCGCTTTAGCATTAGTTGGATGTGATTACATAGGGTAGG
GCAAGAGGTCAGCCACGTGCGACACAGGGAGCCATACGGCATTGTCGGTTTGAGGCTACA
CGCCATTGTGCGGCCCTTTGCCTTACACCTATGCAATCATATTGCCACCAGCTTTCAAAC
GCCCAGCGCCACGGAGAATCCCGTGCTGAGTGGGTTTCGGAGCGGTTCCTTGCCAGCCAA
TGAGGAACCATCAGTCACATTTGCGCGTTTAATTGGAGAGAATTCCTATGAGCAAATGTT
ATTTCTGTACACGTCAGTTTGGTTTGCTGGAGGTTTATTATCCAGTTCGTAAACAAGCTG
TAAAGAATGGTTTGTGGGTTCAGGTTGGGAAGTGCTGCGATGATTGCTGGCAACCAACAT
TGAAACTCAATATCAAAACTTGGGAGAATCAATGAGCAACGTAAAAGGTTTTATCACCAA
CGCTGACCTCACAATGCATGTGGAATCCCAGCGATATATCTCTGAATGGATGACTGGTGC
GCCGTTGGAAATGATGTTGGCAGCAACAAGTCGGAGACTTGTTATTACTTTGGAAGCTAA
TATTCAGGAAATTAAGAATGTTAGCAGCAACCCAATGAATGAAATTCTTGAGGACTTGCG
TCAGCGACTCAGTGAACATCTTGGCGGTAAACCTGTTCCAAACAATGCAACCAAAGAATT
GTTGCAGAAAATTGAGAACATGGCCAAAGACATTGAGAGGGTTTCGAAAGAACGTGATGA
CCTTCAAGCTGTTGTGGATGCTGCGGCAGAAAGCCAACGCCTTGCCATGTTCCATTCAAG
CCGTGCCAAAGAACATCGCGCAAACCTTAAAGAAAGAAAAGGTCGTTAACATGCCGCGTA
AAATTCGAAAGCATTCTGCAAAAGTTACATCTGTCGCAATGAAAGATGGTGATGTATATC
ACTATGTTTTAGTTGGCGGTAAATGGTTTTACAACGGTAGTAAGAACGCCAATATTTCAT
CGGGTGTAGCCAAAGATGGTTTCTGGTTTGACCCAATTGCTGAAATAATCACACGCTCGT
GTGGCGGGTATACAACCAAAACTTTGAACGATGTAATTACTTACATGGGCGAAGAAGTTA
GTCGCAAAGTTTATATGCGTGAGTGGGAATCCAACACGTATTATGGAAATGAGGCACCAT
TTTGATTTACGGTAAATTGGTTCTAACATCCGGTAAGGTGCTGCACGAAGGATTAAACAT
CCAAGAAGTGTTGCAGCTTTCACATGACATGGACGAATGCGAACAAGACCTATCAGAAAT
GATGGGTGGTGATTTAGCACTCGGTCCTCAACTTGTACGTGATTTGGCAAACCGAATCAT
TGAACTGGAGAGCAAACTTGGCTCGCAAAACTCTTAAACAAAAGCTGCGCGCGAAGTTGC
GTGTAGTGAAAAATCTCAACAAAATTGAGCACGAACTTGCTGGCATCACCATCAACAATG
AATGCCAGAAAACTCAGGCTATCGCAATGGCGCATGGTTTGATGGCAACACTGTTGTCTG
TGAAACACAGCATGGGTGTTGACGCAAAAGGTTTTGATGAAGTTGAAAAACTTGCACAAG
GCCGTATTGCTCAATGTATGGTGCAGTTGGTTTCAATGGGCATGAGCGCAGAAGAAGCTA
ATAACAGAATCATGGCAGGTCACTGATGGCTAAACGCAAACAGCGAACTTTCACGGAAGC
CGATGTTAACTTTGCTGTTGCATTAGCTGTAAGTGATTGTGTCAATCAAACTCTTGAGTT
GCTGCTTTTAAGCGGTGCTGAACCTTCCGGCGAAATTGGTAGAATCATTATGCACTTTGC
AAATGGTAAGACCCAAACATACGCCGAAGTTCAGAAAGCGCTCAAAGAAATTGACCACAC
AAATCCTGCAAACATGGAAAAGATTCAAGTCTTGCAGGAGATTGTAAGTGGCAAAGCAAA
ATGAGCAAGACCGCATCATTGCCCTTGAAACGGTGAAGATGTGGTTGCAGGAACATGACG
CCGTGTATCTTGCAAAAGAAAACGTAGTAACATATTGGGAGCACTTCTCACCTGATTCCA
AAAAAGGTGAGTGGGTTAAATTAAAACCATATGAATGTTGCCGCGTCATTAAAGCCACAC
GTGCTGGCTTTGATGCAATGAAGTTGATAAAGCCTGAACTACTGATGCTGGCTGCACAAG
AAAGTGAACGTGCATTTAAGCAAGCAGTATTTTCTCGCTCCAATACTCCAAGTGAGTATT
TCAATCTGTGTCGCACTCAGCATTTCAACCGGCTCGAACTTCTAACTCTCTGCATGTTGC
AGGAATTAGTTGGACGTGGGTGGAACGTTGAAGCAGTGTGTCTCGGCAAACTCATGGTTG
AAGTCTTCTACAAACATGGTTTTGTCGTTCCAAACCGAACACTCCGCTGGCGACTGCTGC
GTGCAGTTGCAGATGAAGCTGGTGTCAAAATCCGCGACAGACTTGACCGCTTAACCGTTT
ATGGTGTAGGTCGATTTGTCTGTGTGCAGATTGAGGGCATCAACGATTCAATCAAAACCG
AATTTACCGAAGACGAAATGGATTCTTTATTAAAAGAAATCGTTCTGCGCTTCGCGCAAA
TTTGATTTTGTTTTTGTTGTAATATTGGAGCAATTCTTCGGCATTAGCTGAATGTGGAAA
TGCTCCATTTCAAACTTTTATTCCCTGATGCGAACACCAACACAAAATCTCAGGTGTCCA
TCTATCCAACATAAGGATTGCCAAAATGGCTAAAGTAGTAACGGTTACTCAGTACGAAGC
AAACGACGGTTCTCTGTTCCTGAGCGAAGCAGAAGCGAATGCACATGATTTCAAACTGGA
AAACGGCGCGCAGATTGAAGCGGCTGCTGAAGCGTTTGTAAACACCATTGGTGCAATCGA
CCGTTCGCGCAACATGCAGGTCAACACTGTGACCAACTTCCTGGCATTCTACCTGCCGTG
GGTTGAAGCAGGTCGTCCGACCGTTGAGCGCACTGTTTTCGATACTCCGAAAGAAGCGAA
AGTGGTTGAAGATGACGCGGCTAAAGCTGCTGATGCAGAAGTTGCTCAGGCTCCAGTTGA
AGCCGGCGAAGAAGCTGCACCACTGTTCTAAAATCGATCTAGCTGGCGTCTGGTAAACGC
AGCGCAAATCCCGATATGATTGTCGGGATTTGAAATACCCGGAATCCCATTCTGCCAACC
ACAGCACAGAGGCTTTGAGCCGATGCCGAGAGTTGAGAGTGGGCTTCTGGATGATTTGAA
TAATCCAATTTGTAAGATGACCGTGCACACAGGACTTGAAATAGTAACTGACTTGAGACC
TCAGTGAATCTCTCAGTATCCGTGAGGAAGTGCACTTGCAGTTGGCTGAAAGACGGATAG
GAACCTCAACTCCTACCGCACTCTTAGAGTGGTCATCCAGAAGTTTTCGTTGATGAGCGA
TTGTTTTTATTCATTTAAAATAAATGCAAACGATTCTGAAATGCGAGTTGCAGCCTAATT
AAATTTAGTCTCGCTCCGTGGTCTTCCGATTCCATGTAACCGAATTCGGCGCAGTGGTAC
GCTGTGATTAATAATGTATGTTTTCTGTCGCCTCCATTCTTCGGAGAATTGGCGCTTTAC
GGTTCGAGTATTCGTAGAGTGGAGGTGAACGAAAGCACTTTGAGTTGCATATTGGATTAG
ATGCGGGAGGATGGCCGATGCCCGTTATGTCATGGCCCAGCAATGTGCAACATCAAAGTG
AATTAAGAGAGCTTTAGTGGTATCTGAATTAACAGACGCGGATTAGTCTCAATGGGCCTA
AGACGGTTTGCGAGGAACACAAACTATTTGAAGTCGCTCTTCAAAGAACGTGTGGTATGC
GCACTCAGATGGAGGTTCGAATCCTCTGTCCGCGAGCACTAAAGAAGAACCGAAAGGTTA
CAAGTAACACGAGGCGACTATGCGCGCCTACTCTTAATAAAGAATTGAAGCATACATCTT
CGGTAAGCCTCTTGTGCACAAAGAGTGTAAGGCAGGTTCGACTCCTCGCATGTATGTTGA
AATATTGAAGATAGACGTAGTTGTGGGACGTATACTCTACTACTACGAGACAACACATTC
ACCCGCGTGTGTCTTCTCTTATAGAAGTGACGGCTAAGACCCGGAGCAAGCGGCTTTAAA
TTTAAATCATGGAGAACACGATGGACGAAAGAATGTCACTCGCGATGGAGTATTATGATT
TCATCATGACTTGCGAAATTGCTGTTGCCTTGGCACGCAAAGACAAACGTCCTGAGAACA
AAACTCTTCGTGAATGTATTAAAGTTTTATTGAAACGCTCGCAGCATAAAGAAGTATCTT
TCATCTTCAAAGGTGTGAAGAAAGCAAATTATCCTGTTGGCGCACTGGCGAAAATTCGTC
GCGAATTCGATGAGGCATTGGGATGACTAATGAAGAACTGAGAATTCAAGCAGAAGCACA
CGGATTTTCTCTGCAAGAGTTTGAATTAAGAATTCAAGCAGAAGTGCTCAGGCTTTTCAC
AACAACTGGAAAAGTCCCGCACTTTGATGAAGCGCATCCAATGTTTAAAGCGGCAGTTGC
AAAATACGCCCAGGAGTTAAAGGCCAAGATGGACGAATTTGAATTAAACGAAATCGACTT
CGATGAAGTTGAACGACGCAACGATGAGAAATTAGCGAATCAGGAGGAAATCCTCGATGA
ATCTGATTGCGATGGTTGCAAAATTTAAGGGATGGATTGCGACTGCATTCGTCTTTTTAG
TTGGTTTGGGTGTTGCCTATCTTTCTGGTAAACAGAAGGCAGAGCAAACAGCCGAAGTCG
AAAAGAAGGATGCAGAAATTGAATCCACAAAAGCTGTTGCCGACACACAAGTCCGTGAAG
CCACGGCTGCAAAGAAAGTTGTTGAGTCTGTTAATCGCTCTAGCGATACCAGTGTTGACG
ACGAGTTGCAGCGTTTCACACGAGACTAGAGTTGTTGATACAAGCTGTTCCAGTTTTAGT
TTGATTATGTTGTCGAAGAAGGAAATTCCTTTATTGACTCCAGAAACCAAACGACAAATT
CTTTCTCATAATTTAATTTATGAAAAGAAATGTGTGCCGCCAAAGAGTGGTACACAGCCT
GAGTGAAATTGTGTGGCCTATCAAGCCGAGGAAACGAACCGGAGTCTTCACTGTTGTTCT
CTCTGGACACAATTGAACGAAAGCAAGCCTCGCAGTTCTCGCTGGAAACCTGCGTTATCA
AAACCGGCAACAAATAATGGTGGCAGAGACTGGTGTCTCGGGTTCGATTCCCAATGCCGC
CCCCTTATTTGCTCCGTGCATTGTCACTGTCAGTCACGATGGTTCTGTCTCACCAAAGTA
GATGGAGTGAATGAATTCGAACTTACCCGACAAGTTGTTCGATACCAGCCCTGAGTATGG
AAAATAACTGCTCAACATAGTCGAGGGAGTGCGCGACTTTAAACAACGAGACTCAAAACA
AAATGAAGCTGCCAGACTTCAGGTGGTCACTTCCTAAAACAGTGTCTGAGAACACACTCA
CCAAAGTTGAATCCCGGTTATTGGGCTCCGGTAAATAAACAAAAGCCTGCCAAATTACGG
AAGACGTGGCGAAATTGGTAAACGCAAGCTGAAATGCTACCGCTGTTGGTTCAAGTCCAA
CCGTCTCCACCATATCCTTCGATACAGTTTTACGTCTGTTCAAAACGTAACATGCGCTCT
GGCATACTGGAGTCAAATCCAGACGGACAGTTTCTGTAACACAATTCAGAAACGCGGTGG
TGAGATTTGTTGCGGTTGCATCGTACCAGACTGTCTAGCAGTCTAACGAGTCAGGCTCCT
CACTGGCGATTGGGTTAACCTCCGAGACGCAACATTGAAATATGCAAGGCAGGGTTTCAT
ATGTCATGGGTTTCTTCATTCTCCCATGAAAAATATGACAGGTGTTACGAGCCTGTGATG
TTCTCCCATATCAAGAAGTGATTCCGCTTGGCCGGTTAGGAATCAAAGTCTTTTTGTTCT
ACACTCTGCACAGTTAAAGTGTACGATAGCTTATTTTCATTCCCGTGAACGTAGAGTGTA
GAACAAAGAGCCATAAGCTGAGTAATCGTTTTAATACCCATTTGAGAGAGAATTCATGAG
CAACAATGATGCAGTTCTGTCGCAGCTTATCGCTGAGGCAGATGAAGCAAATCTAGAATA
TCTTCGTGATGATATTGCACTTCGCGCGATGGAAAGAATGTTAAATCCAGAAACTGGTGC
TGAGATTTATCATCAACCAGTTCATGCAACAAAACTCGCGGTAACAGCTTACGCAATTGC
AGATGCAATGCTGGCGGCTCGTTACATTGAGCCAATCAAAGTTGAACCGGAAGTTCATTA
ATGTCCAAACAAATTTCAGCTGTACTCGTCGATAAGATGGGCACAGATTTAACAACAGTC
AATGCTGCACGTGTCTCATATGGTGCGGAGTCTCACGAATTTAGTGAGCGTGATGAACAT
CTTGTCAACTTTCTTGGCGAGCATAATCATGTAACTCCATTCCGTCATGCTCATGTAACT
TTGCGCTGCCGAGCTCCAATCTTCCTTGCCCGTCAATTGGGTAAACATCAGGTTGGGTTC
TCATGGAACGAAATCTCGCGCCGCTATAAAGATGGTGTAGCGATTGAGATTGAAACTTAC
GAACCAGAACTTGTTCTTAGTCGCCCGCATAAGCTGATGACTCAATCTGCACAACCCATG
CGTGAAGACTACGCCAGTGAGATGAAACTTCTCATTGCTGCGGTGCAACGTGCGGCTGTT
CGTGAATACCAAGGTTTAATTGATTTGGGTGTTGCTCCAGAGCAAGCTCGAATGGTTCTT
CCACAAGGTATGATTACAACTTGGGTGTGGACAGGAAGCCTCTATGGTTGGGCTAACTTG
TACAAGCAGCGTTCATCAGAACATGCACAGTACGAGGCCCGTCTGTTTGCAGAAGAAGTT
AATGTAATCATGAAAGAACATTTTCCTAGAACTTGGAAAGCTTTAACAGGACAAGGTTAA
TGCATAAGACATTGCCAGCAAGATTGTATCCTGCGGCAGTATCTGCAAGGCTGTATAGTC
CATATTTGGCCTCTGGATATCGCTACTGGTATCTAAACATCTGCGGCACTACATACTGTG
TGGCAGAACACAGGTTGAACTACTTTATCAAGTCTAGGAAGGTTGTTGAGAAAGTTGACC
ATGTGGATAACAACAAACTCAATAATGCTTTGGATAATTTGCGTGAGTGCACAACTTCGC
AGAATAACCACAATGCAAAACTAAATGCACGAAACACTTCCGGACATAAAGGAGTAAGTT
TCAACAAGAGGCTAGGCAAATGGGCAGTTAATGTCTGTTTGAATGGTAAACTACATAGCG
GCGGTGTTCACGAACATCTTGAAGATGCGGTGATTGCTGCAACAACTCTGCGAAAGAAAC
TCCACGGAGAGTTCGCCAGAGACTATTAAGGACTCCAACGAGATGCAAGTCAAACAGTGT
GTAGCCAAAATTAAGCACGATGTTCCCACGTGTAAATCAACAAGCGGGAAATCCCTGCAA
GTTTGGTTGAACGAAAATGAGGATGGAACTCGCAATTATTCTGGTTATTGCTTTGCCTGT
TCTGTTCTCGTTCCCAACCCTTATGGTGACAATCCACCGGACCCAAAAGAAATTCACGTC
AAAACTCCAGAAGAAATTCAGGAAGAACTTGATGAGATAACTTCTTGTCCGGTGTTTAAT
CTCGACCATCGCGGCATTGAACCAGAATACTGGAGCCATGCTCGTGTTCGACTTCTGTAT
TCGGAATTCGATGGTACAACTCCAAATGCTTTAGCCCACGGATATACTCGTGGCGGCAAA
CTTGTTCGTTGGAAGATTAAACTTCTGAACAAGAAAGTGATGTGGAGTGTTGGTGATACT
CAGGAGAATGACCCATACAATTGGGAAGCTGCCAAATTCATTGGTGGTAAAACTCTGTAT
ATCACAGAAGGTGAAGAAGATGCGATTGCATTGCGTCAGATTCTCCGCATCTTGAACCGT
GGTACAGCATATGAGGAACTTGATTTCGCAATCATTTCCTTATCAGATGGTTCAGATTCA
GTTCATAAATGTCTGGCTCGCGTAGCCGAAGAGATTAAGCAACGCTGGGAAAGCGTGGTA
ATCGTCTTTGACAATGATGAACCGGGCAAGAAAGCAGCGAAGGAAGCATGCCGTTTATTA
CCGGGTGCAATGGTTGCACAACTCCCGGCAAACGATGCCAATGATTGCCTTAAACGAGGA
ATGTTAAGACAAACTCGTGATGCGGTAATCTTCCGTGCTGCACGTCCGTTGCCAACAGCG
TTGGTGAATAAAGAATTATTAATGTTGGAACTTGACGATGAAGTTCCTCCGGGTGTTGAT
ACTCCGTGGTCTAAATTGACAGACATGATGTTCGGTCAACGTCGCGGCGAAATTATCACA
CTTGGTGGTTCAGAAGGTGGTGGTAAAACAACACTAGCTCGTCAACTCTCAGAACACAAC
ATCGTTAAACATGATTGGGGTGTGTTCACTTGTTTCATGGAGGAAACTCCACAAGAGACA
TTGCTTCGAATGGCTGGTTTGCATGATGGACTCCCATATTGGGAACCTTCATTCAGACAA
GACCCACGATTCGATGAAGAGAAGTTCCGCGCAACTTGTCGTAAAATGCTTGCGAACATG
GAGATTTGGGATAGAAAGCAACAAGGTGAAGACCCGTATGAAACATGGGAAGGCATCAAA
ACTATCCTGCGTCAAATTGGTCCAGACATTGACATGTTTGTCTTGGATAACTTAACAATT
TTGTCAGAAGGTATTTCAGCGTCTGAGAAGAATGATTTCCTCGGTAAGCTTTATGCCGAC
CAAGTTAAGTTAAGCGAACAATATCAGTTTGGTATCCTCGACCTATCTCACCTTAACCCG
GTTCCGAAAGGTCAGCGTCCTCATGAAGATGGTGGACGTATTAAGAAGGGTGACTTCACT
GGTTCTCGTGCAGCATCAAAATATTCCCATTTCATGATTGGTTTCGAAAGAAACAGTCAG
GCGATTGACCCAAACTGTTCAATCATTCGCGGTATTAAAGCCCGTAAGAGTGGTAAGACA
GATGGTTTCAAAACATATTACGAATCAGATTCTGGTCGTATTATTCAACGCAATTGGGAA
GATAGTTTGTTTGAAACGAAAGAAATCGTTCAGCTCACTAAGAAAACAACCCCGCACCAA
TAACTAAGGTCTTGTCCTCCTACTGTTGTGAAACAGAAGCTTGGACAAACCTCCTCAACC
ATCCTTGGAGACAAAATTGATTTATCCTTGGGAAAGAATGTCTGCCAGTGACATTGAAAC
GACTGGTTTGTTAGAGCAAATGGCGAAACAGGAACATCCACGTTTGCACAACATGGGTGC
GATTGATGCATTAACGAAAGTTGAAAATTGTATGGAGTGGACTGAACGTAGTAGAATCCA
GGCATTTCTCGACACGGCTCCAACTCTCATCATGCACAACGGTTTGTGTTTTGATAAGGA
GGCATTAGCCTTCCTTGGATATGATGTATCCAAAGTAACGATTATTGACACTATGTTCAT
TAGCTGGTACCTGCAACCTCGTCGTATGAGGCATGGTTTGGCAGAATACGGTGAAGAATT
TGGTGTACCAAAACCTGTAATTGAAGATTGGGAAAACCAAACTCAGGAAGAATATAACTT
CCGTGTAATGCAGGACTGCCGTATTCAAATGGCATTGTGGGAACAGCAGTATTCCCAACT
CTTAGCGATTTATCGCACCCCGGAAGAAGTGAAACGCTTTGTCGAATACCTGATGAGCAA
AGGCCGTCAGCAAGTCATTCAACAACGTACCCGGTGGAAGCTGAATGTACCGAAAGCAGA
AGCTTTTCGCGACATGCTTGCGCCAATGATTTCGGAGAAAGAAATTGCACTCGAAGCGTC
GATGCCAAAGGTTCCTAAGTATGCACTTAAGAATCGTCCAGCCAAGTGTCATAAGCAAAA
TGGAACATTGTCTGCAACAGGCGCCAAATGGAAAGCAGTCTGTGATGCAAATGGTCTTGA
TTGGCAGGACAAAAACCTCGTCATAAAAGTTTTAAAAGGTTACGACGAACCAAATGCTGC
GTCTCATGCTCAAGTAAAAGATTGGTTATTCAGTCTTGGCTGGAAGCCGCAAACATTCAA
GTTTGTTCGTGACAAGGAAACAAATGAAACTCGTCAGATTCCACAAATCACAGTTAAGGA
TGATGATGGTATTCCAGATATTTGTCCGAGCCTGCATGAACTGGCGGAAGATAATCCCGG
CAAAGGTGTTGAACATCTTATTGGCCTCGGTGTATATCGTAACCGCTTGTCTATTGTCAA
CGGCTGGCTCCGTGATGTATCCGACGATGGCTTCCTTGTTGCTAGTTGCGCTGGTCTTAC
TAACACGCTGCGACTGAAACATCGTGGTCTGGTAAATATTCCATCCATTCGTGTGTTCGG
TGGTAAAGAACTTCGCGGCATGTTGGAAGCACCAAATGAAGATTATGAACAACTTGGTTC
AGACCTATCTTCACTTGAAGACCGTTGCAAGCACCACTTCCAGTGGCAGTATGACCCTGA
ATATGTCAAGAAACAGTTGGCACCAGACTATGATGCTCACTTGGCAATTGGTGTAATCGG
TGGATTCATTACCGAAGAACAATCACAGGCACACAAAGATGGAATCAAGAAGGTTCCTCA
ACGTCCAATGTTCAAAACCACAAACTATGCTTGTCAGTATGGTGCGGGTATTGCGACAGT
GCAACGTGGTGCGAAGTGTGATTGGGCTACAGCTGAGAAGCTGCATCAAGCTTACTGGCG
TCTGAATTGGTCAATCAAAGAAGTAGCAGCGAACACAACTGTGAAAACGATTGATGGTCA
GATGTGGCAGTTGAATCCGGTAAATGGTTTCTGGTATTCACTGCGTAATGACAAAGACCG
TTTCTCAACTCTTTGTCAAGGTACTGGCGCGTATGTCTTTGATGTTTGGTGCAATGCTGT
AATTGAAATTTGCAACGAACGTTATGGACGTGACCCAATGCTGTCTGGACAATTCCATGA
TGAATTGATTCTGCAAGTTCGCAAAGGTAATCGTGAACTGTGGCAACAAATCGTGGGTGT
AGAAGCAATGGAACGCACCAATAAACAATTGAAGATGAACCGCGATGTTGCGTGTGAAGT
TCAATTCGGCGACGACTATTCAGAGATTCACTAATGGCTAAACTCATTTACGCGTTGTAT
GACGCACAGGAAATGACGCTGGTTCAGTTTAGTGCAGATGCCACCATCCTTGCGAAGGAT
GCTGTGGCAGCATATCTGACAGAGAAAGTTATTAGCAAGACAGTCGAAGAAGATTGGGCT
AAAGATTTCCTCACAGCAATTTCCAACTCAAAGGATGTTCCAGAACTTTTAGTTGTTTTG
GAATCCTATGATTTCAAACTGGCAATCATGCCAATCCACTAATTTGCACAAAACTGTTTC
AATGGAGAAACAAAATGGCCCTATTCCAAGCACCAACCAAAGATTCCGGTAAAGCACCAA
GTAAAGCACCACTGTTAGAATCAGGTGGTTATCCAGCCCGTCTGGTACGTCTGATTGACC
TCGGTAAACAACCGGGTTCAGGTCAGTATCCAGAACCAGCATACAAACTGCTGGGAACTT
TCGAGCTACTCGATGAGTTCATGAAAGAAACCAACGAAGCAGGCGAAATCATTATGGTCC
AAGACCCTGATGGCGAGCCAGGCGAAATGATTGGTAAAGACCTGCTGGACAAACCACGTT
GGTTTGATTTCGAGTTTACCTACAATCCAGATGGATTTATGGGTGACCGTTCTCACATCT
ACAAATTCATGGCAGCGGTTGACGCATTTGAAGTTAAACCGAATCCAGAAGCTGGTATTG
CCGGTCATCCAGCCAAACAGTTGAATGAACTGCTGGGCGAACCACTGGTAGTTAACCTGA
CTCAGTACACCAAGAAAGGTGGTAAGAATGCTGGTCAGATGGCAAACAAAATCTCAACCT
TCGCGCCAATGAAGTCGAAAGACAAACGTGATGCGAAAGCTCTGGTCAATCCAACCATCT
TCTTCGACTTGGGTGCTCCTGACCTCGAAGTATTCGGCAAGCTGCCGGGTGGCGACCAAG
AGTGGGCTGTTAAGAACCGCATTCTGGCAAACTTGGACTTCAACGGTTCTAAGTTGCAGG
AACTGCTGGGCATTGCCAAAACTCCAGATGCACCTGTTGAGAACAAAGCAACTCCAGAAC
AGGTTGATGAAGCTCTGGCTGCTGAACTTGCTGCGCAGGCTGCTGCGAAAGCTAAACTGG
CTGAACAGAATGGTGGCGCTGATGCCGCTGCTGGTCAACCTTTCTAAGTAACACACTGCA
TCTACGTTGGGTAGATGTATTTACCAATTGGTAAAAGGGCGTAACCAAGATTGTTGCGCC
CTCTTTTACTGGAGTTTTGCAAAATGAGTAAGAAGAAAGTTCTCCTCCTGACCGATGGAG
ACATGGTGGCATTCAGCCATTGCGCGGCAGAAGAGTACGGGAAAGAGTCCGAAGATATTT
CTTTTGCAAAAATTCAGATGTCTATGGAATCCAAAATGGAATTCATGGAAAAGCGTTGTC
AGGCAACTGAACACATTTGTCTGATTTCTGGTGATTCAAACATGCGCATGGTTATCGCTG
ATACATACAAAGCAAACCGTGATGGCGTATGGAAACCAGAGAATTTGAAGAATGCCAAAG
CAACTTTGATTACCTGTTATAACGGTATCAAGAAAGATGGTCTGGAAGCTGATGACCTGA
TTGGTATTCTGTGTCGTAATAAAGTCGAAATGACAATGGGTAAACGTGGTGAAATCAAAA
CGATTAAAACCATTCGCCCATTGACTCTTGATGACTATGACGAAATTTGGGTTGCATCCC
TCGACAAAGATTTGAAACAAATCGGTGGTCGTCCGGGTGGTCCAGTTGTCAAACATTATC
GTTGGGAAACTGGCACACAAGGTGAGAAAATCACTGTTGTCTCTGGTTTCGGCGAACTTA
AATGTATCGTCAAAGATAATGGTAAAACCAAGAAGAAAGAAATCAAAGGCTTTGGTCCGA
AGTTCTTCCTTTGGCAGTGCCTCACTGGTGACTCAACCGATGGTGTAATGGGTTGTGGTG
TTAGTGAAACAAAGATGTACAAAACTGGCGCAAAGGCTGGTGAAGAATATCAAAAGCGTG
TCGGTGTCGGTGCTGTAGAAGCATTCGAATTGCTGGACAAAATTGACAACTATGCAGACG
GTTTGAAAGCCGTAGCTACACAGTATGTTATGCGCTTTGGTGATGGTTGGCAGGAACAAC
TTCTCGTGAACGGCCGATTGCTATATATGTCAAATACGGTGGCAGAAGGAAACAAGGTTC
GACTTTGGCATTATAATCCACAGGTTGTGGAATATTTCGACCTTAATTCTAAAACCATTT
CGGCGTGATTTTATTCGTGTGGCATTCTGCTTGAGCATTAGTGGGATGTGAATTTGCCAT
GTATGGTTATTGCAAAACCGAAGCCAGGTTTACAGCTTGGCTTCAATCAGCACTGAGAAG
TGTTTGGGCAAAGCATCCATCAAAACTTGCACTTCTTGAGAAGAAGCGAGTGGCACTGAG
GGTTGGCAACAGTAAGAAGCCAATCTATCATGTGCAGTGTGAGCATTGCAAAAAGCTGTT
CAAGCTTAAAGAAATCGAGGTCAACCACAAGAACAAATGTGGTGGCCTTTCGGATTTATC
CAAGCGACATGAATTCATGGACAATTTATTATTGGTTCAGGAATCAGACTTGGAACTTCT
TTGCCATGATTGTCATGGGATTGTAACTTACATGGAGCGTTACGACGTTACCAAACGAGA
AGCAATCATTGAGAAGAAAGTAATCGCATTCTATAAATTGTCTGATAAAGAACAAATTAA
GAAATGTGAACTCGCTGGTTTTAATCCAGTCCCAAAAACCAAAATCGGGCGCAAGAATGC
GGTTCGAGAATATCTTAAGAAGGTACTCCCGCAATGACTATTAAGTTATTGGACGACGGA
ACATCTTATACGAAGGCACATGCCCATGATTGGGAAGACCCAAATTGCCAACTCAAAGGT
CGTTGCGAAATTTACATCAAGATTGATGGGATTCGGGCGATTAGGAATAGAGACGGACGA
GTTTGGAGTCGCAATTCCAAGCCTTTACCCCATCTTGACCATTTATCTTTCAAAGATGCT
GAAATCTTCCGCGATTCATGGAATACGACCTCAAGCATCTTGGGCAGCGATGCTCCACCA
GCGTCGCCGTTAACGCAGGAAAACGTGTACGAACTGTCTGATGGCAACATCGACAAACGT
CTTTACCTCGGATGGGCTCAAGACCCAAGCAATGAAAAGCTTCATGAGTTGATGCTGAAA
ATGCTGAAACTTGGTCATGAAGGCCTCATCGTTCGTTGCAAGGATAAGAAAGGAAAAACC
CTTTGGTGGAAGATTGTACCATATAAATATGCCGATGTTAAGATTACTGGCTTCAAAGAA
GGCACTAATAAGAACAAAGGTGTATGTGGCTCAATCGCAACGAACTACGGTTCTGCTGGC
TCAATGGTTTTTGACTGTCTTCAAGACCACAACATTGTTGGCGATTACAACATTCGCAAA
TGGTTGTGGGAACATCGTCACGAACTGATTGGTAAAATCATTCAGGTTCGTTACCGCGAA
GTAACGGAAGCTGGTAAATTCCGCTTCCCATCTTTGGTACGGTTACGCACTGATAAGTCA
GAAGAGAGTCTCGACTGATGCAGAGAATCAAACTCGGTGTGGTCTTAAATGGCCCACCGG
GCTGTGGCAAAGATACCATAGCCAATAAGATTGTCGAGCAGGGCGTGTTCCGTAAACATC
AGTTTAAGGATGCACTATATGCGCACACTGCTAAGCACTTTGAGGTTGATTTGGATAAGT
TCATCCACTTCGCCTCTGACCGTGTACTTAAAGATTCGAAATCCTTGGCGGGATTGGGCG
GACGAACTCCTCGTGAAGCGCTCATCTATGTCAGCGAAGAAATCTATAAGCCCCGTTATG
GCAGCGATTACTTTGGCAAAGTTGAAGTTAATCGTGTGGAAACACTCTGCGGGCATATGG
ATGGCGATGTTAATGTCATCTATCCCGATGGTGGTTTTGCAGACGAACTTCGCTGCATTG
ACACCTGCTTTGACGGCGTGCTTGTTATTCGTCTTCATCGAGACGGCTTTGATTTCAAAG
GCGATTCTCGTTCATACGTCGAACTGCCAGATACACCAGAGCGTAAAAGCATTGATGTGG
ATTTAGCAGACGACCAAATTAATATTGGCGTTCACATTGTGGAATCAGCCATTAAATCAT
TCAGAGAGTCTCTGCGTAATGTGTCCCAATAAAGTCCCTCACGTCAATTTCCTGCTTGCT
CATCCGCTGGCTCAACTTCCATCGTATGAAAGTCGCGGCGCTGCTGCTGCTGATATTCGT
TCTGTGGAAGGTGCAATTATTTATCCCGGTGATACTGCCATTGTTGGCACTGGCCTTAAG
CCAGAAATTCCCGAAGGTTACGTTGCACTGATTTACTCACGTAGTGGCCACGGCTTCAAG
AACGATGTTCGTCTGGCAAACTGTGTCGGTGTTATCGACTCAGATTATCGTGGCGAAATC
AAAGTCAAGTTGACCAACGATGGCAGCGCTGTCTTCCATGTTGAACCACTTGACCGTGTT
GCACAAATGATGATTGTTCCTGCTATTCAGGCAACATTCGAAGTGCAGGAAAATCTGACC
TCAACTGTACGCGGTGAAGGTGGCTTCGGCTCAACCGGTCGCGCGTAGTTGCTCAATGGA
GAATCCGAGCATGTGTTTACAACTCAAATTTGAAAGTCTTCTGTGTCATTTAAATGCAGA
AGAGAATGATATGGCTCTGGAACTTCTGGACCAAATCAAAGAAGCTGATGTTCGTCTCGA
AAAGTTAGATGCACTGGAAGCAGGTGGTGTTGATAACTGGGGCGGATACGGCGACTGCTT
CGAGGATGATGACGATGAATAATCTGAACCATACCTCAACAGGACTTGTTGGTGGTGACT
TCATCAAGTTCCTGAAATACCTGTTTGACGATACTTTTGAAATTGACGCGACTCCTGGAA
AACATAGCTCAAGCAATGTTACTTTAGGTGTTGAAGTTAATTTAGAAGTTGCAATTGATA
TGGTGGATGAATTCCAACTTCCGATTGCTGCCGCTGATTTAGTTGGTACTTGGGCTATGG
ATGCCTCGTCAGACTACAGCTGGGGCATTGAATGGAGTATGGTTGACTGCGCGTATCGAG
TTGAGCAGGTTACTGAAACCAAAATTGTAACCTCATGGAAACCCGTTCAAGATGCTAAAC
CAGCCACAAAAGAAAGTGTACCTGCGCAACCCGCAAGTAAAAAATAATGTCGGCGTAAAC
TTTGTTTACCTCCGTTCAACAGTCACCTCAGCCGGTGGCGGAACTTATATCACTCGTGCG
TTAGATGCCCGTAATAAAACTGGCGCTGATGTAATTGTGTTTATGATTCCGAACTCTGAG
AATGCACTGCGGAACATTGAGCGTATCAAGCGTGGCCGTGAAGATTTAATGTCAGCGGTG
AAGCCGCTTATCTTGCCTTTCAAAATTGTAGCTGTAATTAGCGAGAATCAGAAAGCACTC
GAAGAACATTTCGAAGATATGCTGTGGAACGATATTAAATACTAAGCGCACGCCGATGGT
TGGGAGGTCTCTTTGGCCTCCTATCTTCTTGGAGTACGCTTATGCGCACTATCACATGTA
AGGAATGTGGCTATGCTATTGGTGAAGATGACTTCTCCTTAATGCAATGTCCAGTGTGTG
ATTCATTCATGGAATTGAATCGCCTGCAAAAAAGAATTGAACCTGATTTAGAGAAGGTTA
TTAGAATAGACGCCACTAAGCGATTACCAGAAAGGCAAACAAATGGATAACGATTTAGGT
GATGAATACAACAGCACGGTTGTCTATCATCTGTTGAAGTTCTACGGCGACTTATTAAAA
GTTAGCCGTGCACGCTTTTGTATGCAGTCACTGCCAATCCTTCGTAAGTATATCGCGGCG
AATCCAGACATTATCAGAGATTACTCTGAGCAACTGGATGCAGAGATTGAAGGACAGGGC
TTAAAAGATAACGCTGTCCTGCGCTCTTTGGTTACAGCACAAAGAGAAAGGTTGGAAGCA
GCCGACTCTAAAGATTCCACTGCGTATGCCAGTGAAATCGAAAAGATGATTAAAGACGGT
AAGACAGACCGCATTGCTGGCTCAACAATTGAAGCCGACACTGACGAACACTCTGCAACC
GACGAACTCATCCAGCAAATTCTGACTGACCCACTCAACGTAGACCCAACTGTTGTACCG
AAAAAGGTAGCAGATGAAGTCAGAAAAATATTAACTTCAAGTTTTGAGAAGTTTGGCAAA
TGGTCATTTCATATCCAAATGGGATTCCCATTTCAGTCTCAAGACTTCCATGATGTTATC
TTCGATGTAGGTCAACAAATAGTTGATGGTAAAATCGACCGTGTGATTATCACAATCCCG
CCTCGACATTCGAAGACTCAACTTCTATCCATTGCATTGCCGTTGTATTCATTCTGTCAT
AACGTGACGAGCCACAACATCATTACTTCCTATGCGGAAGACGTTGTATTGGAATCCAGT
GGCTATATCCGAACTGTAATGCTTGACCCATTGTTCCAGCGCATCTTCCCAAATGTTCGC
ATCGACCCAAACAAACGTTCTCTTGAGCGTTGGGGTACTACGAAGCAGGGTGTGATGCAC
GCTGTTCCAACGGGCGGTAAAATGACTGGTAAAGGTGCTGGCCTGTTGGCTGAGAACTAT
GCCGGTTGCTTTGTAGTAGATGACGTAATCAAACCAAAAGATGCGTACTCTGATACAGTT
CGAATGGAAATTAATGACCGTTACGACAACACATTCATGTCTCGTTTAGCCAACGATGGT
GTGATAACGAAGCCTGATGGCACGTTACAAAAATGTGCTCGTACTCCAATGGTCATCATC
ATGCAGCGTGTTCACGACAGTGACCTTGTTGGTTACTTGCTTCGTGGTGGCTCTGCTGAT
AAATATCATTACCTCAACATTCCAGCAATCGTTGAGAAAGAAACTGGCTCTGCTGCATAT
TACGATAAGATTATTAAGAAACAGGCATACACTCATGCGATTCCAATCCTCTATGATTTG
AAACGTACTGAGGAAAAGTCTGCTTTGTGGCCTTCTCGTAAAAGTTTGGAATCTCTGGAA
GCAATGCAGAAAGCGAATCCATACACATACAACAGCCAGTATGCTGGCGACCCAACTGCA
AATGGTACAGGTCTGGTCAAAGAAGAATGGTGGAAGGAATACAATCCTGAAACATTCGAC
CGGAGCCGTATTGTCCGTTCATTCATCACTGCTGATACCGCATCTACTGCGAAAGAATAT
TCAGACTACTCAGTGTTGAAACATTGGGGCGTCACGAAAGAACGTGATGTATATTGCTTG
GATATTATGCTTGGGAAATATGAAGTTCCTGAGTTGAAGCTGGCAATAAACGAGTTCTGG
AAGAAGTGTAATATCTTTGATTCAAGATGGCCTTGTTGTATTCCCCGTGCATTGTACATG
GAAGACAAATCATCTGGTCAATTCCTAAACCAGCAATTTGTTCGTGATGGTCAAATCAGG
CTTATGCCTGTTCCTCGTGACAAGTCTGGCGGTGATAAGGTAGCACGATTCCTTAACACC
ATTCCATATTTCTCAACTGGCAAGATTTATTATCCTGCTGGACATGAACATCTTGAACAT
GTTAAACGTGAAATACTTGGGATGACTTCTTTGGGTTCAGGTACTGGACACGACGATTGT
TGCGATAACGTCTCAGACATGGTCGCAATTGAATTCTCCGGCAACAACGCCAACTACTCT
GCGTGGGTTTGAAATGAGTGTGATTACTCGCATTGATGCCCGAAGCAAAAACAATACTGG
TTTCGAAATTCGAGACGCAGTAACTGGCAAAACGCTGGTGATTGTCGAAACTTTGGGCAC
TGAAACTGAACTCCGTATTAGCTCAAGCGAGGCAGTAGAAATCGTGAAAAGCAACGGGGT
TAAACTCAAAAGGAAGAAATAATGTCTTCCAAAAAGAATCGGCCCAAGGGCTCGATTAAA
GTCGGTGACGGTCTTGAGAATGTCATAAGTGGTATGGGTGGAGACAAAGATAAATCGGTT
TATAATCGGTGGACTTTCTCCAACAAAAATGCAGACTACCGTCAGCTCATGAATCGCTTC
CGCGAAGATTGGGTTAGTCAAAAAGTCTGCACCATTATTCCTCAAGACATGACGCGAACT
TGGCGCAAAATTAGTACGCCAGAAGGACAGTTGGCAGACAAGAAATTGAAACTGCGTAAG
CTGTGCCGTGAAGGCTACCAATGGGCAAGATTGTACGGAACATCATTTATGCTTCTCGAC
CTCAAAGGTACGGGCAGACTTGATACTCCATTACGTCTTGATAATTTGAAGAAGGGATGT
GTCAAATCCCTGAAAATTATTGACCGTTCGCGACTACATGCGGCTGGTGGTATTGTACTT
GATGCACTTTCACCTCATTACGGTTTGCCAGAGTATTACACTCTGAGCGGATACCCTGGG
ATGATTCATCATAGTCGTTTCTTACGTTTCGAAGGAACCGAACTGCCGTTGTTCGAATTC
CAAAGAAACATGTGGTACTCGGATTCAGTGTTGATACCACTGATGAGCACCATCGACCAG
TTTTACACCACTGCTTCTGCTGCCGCACAATTGGCACAGGAAGCAACCATTGATGTGGTG
ACGGTCGCAGGACTGCAATCTCTTCTGACAAATCCAGAAGGTGAGCGTTCAGTAATGAAA
CGATTCCGACTCATGAAGCAAGCCAAGAGTTTGTACAACGTACTTATTCTTGACGAGTCG
GAAGAATACTCCACCAAGTCTATCGCTTTGTCAGGCGTAAAAGATTTAATTTGGGAATAC
TTGAAAATCGTTGCGGCAGCGGTAGGTATCCCAGCAACACGTTTTCTTTCAGCATCGCCA
GACGGCATGAATGCTACTGGTGAATCCGACCTTGTAAACTATATTGACTTGCTTACTGGT
TTACAGACTGCAATCTTCGAACCTCGTCTTGAGATTCTCGACACGATTGCATGTGCCCAC
TTTGGGATTGAAGAATTCACTTATGAATTCTGTGATATCTTCCCTGAGTCAAGCGCGGAG
AAAACAAAACGTGCATCTGATTTGGCCATTGCGTTGGAGAAATTGATTGAATCTGGCACC
ATCACTCGTGAGAATGCCAACAAGATTCTGGAACACAGCCATGTATTTGGTGCGTGCCCA
ATTGACCCTCCACCGGCTCAACCTCCAACTCCGCCAAAAGGTAGTGGGAATGCAAATTAA
GCAAAACCAATTCGTGCAACTGAACGACGAAGAAACTGTTGAAGGCGGTGTCGTTCTTCT
GACCGACAAAGCAACTTTAACAACTCGTCGTCTGCGCGATTCTGGTGAAATGATTGCGGA
ATGCACTATTGCTCGCACTGGCATTATGCTCTATAAAGCCAAAGAGCTGGGTGAAGTTGC
TGCACATCTGCCACCAGACCAAATCTGTCGTGTTCGCACGAAGCCAGAAGTGTTGTTCGA
TGAAGCGACCATTGAAGGTTGCCGTTCGATTCCAATCACTATTGGTCATCCGAAACAAGA
TGTAAGCATTAAGAACAATAAGGAATTGCAGAAGGGCTTCATTGAAGGTCGTCCGGTTGC
TGATGGTTCTTTCTTGGCTGCATCAATTGTCCTCAATGACGAATCTGCGATTCGCTTAGT
TGATTCGGGTGTTGACCAATTATCATTGGGCCACAACGCTTTGCTGGAAGCCTCCACGGA
CGAAGGTGTTGACTTCGATAAAGTCAAAATCGTTCCGAATCATGCTGCAATTGTTGTGCG
TGGTCGTGCTCAAACAACTCGCATCGGTGATTCCGGTGAAGAGATTGAAATTGTAGACAA
GACTTCTTTCGATGCACTCGAAGCTTCTCGCGACGAACTGCAAACCAAAGTTGATGCCTT
GACTACCAAACTGGCTGATGCTGAATCTGCCCGTCTGACCGATGAAGAAATTCAGACTCG
TGTTGATGCCGCTGTCAAAACTCGTTTTGATTTGCTGGTTAATATTGCCAAACTCGGTGA
TGAATATGCGTCTATGGACTTCACTGGCAAGACCGAAATGGATATGAAACGTATGGTTGT
TGCGAAACTTCGCGATGCCGATATGTCAGAGAAATCTGACGAATACGTTAATGGTCGTTT
CGAAATGGCGCTCGAAGATAGTGATGAAGTCACTCTCTCTGATGCACTCTCCGTTTCTAT
CAAGCACGGTTCTCAGGAACAAGCTGAGAAGAAACTAAGCCCACGCGAAGAAGCTCTTGC
TCGTCGCCAAGCTCGTTTTAATAAATAGGATTCAGTCAATGCCAAAGCAAGACTTTAACA
TTAATACACTGCCTGCTGCTGCTGGTGACGTGTATGGTTTAGCTTTCACCAACTCAACCC
GTCTGACCTTCTCGACCGAAGATGCACAAGCTGCATATGGTCTGGCAGTTCAACAGGGTG
CGAAATCCAACACCATCAAAGTCGGCCATGTTACCGGCGAAGTGTTGGGTATTACCATGC
GTCAGAACGTGCTGGAATCTAAGACACGTCCGGGCGACGGCACCATCAACATTCCAGTTG
GTCAACCACTAGCTGTAATGATGGAAGGTTCAATCAACATCGTGCTGAAAACTGCAATCA
CTTCTGCGGATATTGGCGTTAGCGCAACTGGTGAATTTGGCGGCGTTGCTGCTGGTTACA
CCAAAGCAACTAACATCAAAGCCCTGAGCTATCCAGCCAACGCTGGTGATGTGATTCCAG
TTCACATCGTTGTCGCGCTCCCAAAGTAGCAGCCCCGGTCTTACCTAAAACGGTAACCGG
TAAAGGTGATGTTACAGGTAAGAAAGGCGGGGATGTAATCGCTGACACGGCTCTGTTCGA
TTATTCGAATGGTGCAACTAAAGCTGATGTAACTGCTGTAACCGGCACCAAAGTAACTTG
GGATAACACAGCCAAAACGCTGACTATCGCTGATGATGTTACTGCTGGCGACATTGCTCT
GGCCTTCACTCTTGCGAGTGGTGTGACCAAAGCAGGTACTGTTACTTTGAAAGGTGTTGC
GGTCAAGCCGTAACCTGAATTAATTGCGGCCAAATAACTTGGCTGCATAATTGGATTGAA
TAGATGCCAAAGCTGATTAAACTGAACGATGGTTCAGAATTCGAACTGGATGATGCACTG
GTTGCTATCCAGTCTAACGCCTCAGTAACACTGAGCGATGACGATGCTGTGTTCTTCCAG
CGTCAACTTGAGTTCATTGAAGCTCAAACTTACGACACTCTGTACCCTGACCTTGAAGCT
CGTGATGCGCTGGGTGTTGATTCAACTGGCGGCGCTGGTGTTCAGACTCTGACCTATCGC
AGCTACAACCATGTTGGTAAAGCGCAAGTAATCAATGCTCGTGCAACTGACCTGCCGAAA
TCAAATATCTCTGGTAAAGAGTATTCCATTCCGGTGAAATCAGTTGGTACTGCATACGAT
TACGACATTGACGAAATCGCATCTGCTGCCGTAACTGGCCTGCCGCTGGAAGCGCGTAAA
GCAATGGCATCTACTCGTGGTTACGAGCAGTACATTAACTCTGCTGCATGGTATGGCGAT
GATGCCAATGGTTTCATTGGTTTCTTCAATAATCCAGATGTTACCAAAGCAACTGTTGCT
GCTGGCGCAAACAGCAAAACTGGCTGGTTCGATGGCAAAACTCCAACCGAAATTCTGAAA
GACCTGACCACAGCGGTGAGCGCAATGTACAGCTCAACTCTGAAAATCATGCGTCCTGAC
GAAGTTTGGATGCCGGTTCTGCATCATCAGTACATCATGAACACTGCACGTTCTGAGCAA
TCAGACATGACCATCTTGCAGTTCTTCATTGCGAACAACGAATTCATTAACTCTAAAGAG
AAAGTGAAAGCGCTGAACGCAATCAAAGGTCACGGTGCTTCTGGTGCGGATTGCTTCGTT
GTTGTATGCCGTCAGGTTAATGGCCTGAAAACCTTCCGTCTGCGTGAGCCACTGGCTCTT
ACTTGGCAGCCGGTGCAGTTGCACGGTCTGGTATATGAAATCCCAGGTCGCGGTCGTTTC
GCTGGTTTCCAGGTAATGTACCCTGCTGCAATCTCCATCAACTCAGGTATCTGATTGGTG
AATGGCGGGTGAAATATCCCGCCTTTGTTTAATTCGAATAAGAGAAAAAGATGCAACTTA
AAAACAACCTCAGCGGTAATGTGCAGTTGTTCTATCCGGTTAAGAAAAATGGTCAACCGG
ATGTTGACTTCATTCACATTCCCGGTGGTGCAACTGTTGAGTTGGATGACAAAGTCTTCG
AAGCTCTCTGCAAACCTCTGACAACCATCAAAGAACAACGCATGGTTGAAGTAGAAATTG
AAGGCGAAGCGGAAGTTAAGATGGACAAAAAGAATGTCCTCATCAAAGAGTTCTACGAAA
CCGGCGAAGTTAAAATTGTTAACCTGTTCAAAGAGCAGATTAAGAAAGGCGACTTCACTG
TTGTAGAGCGTCCGTCAATTTCCAAAGAGCAGAAACTCGCTGTCTTGAATCAGAACGGTG
TTTCCGTTAAAGATTTGAGCGACGAGCAGATTGATGCTCTGTACGACAAACTGGCGTAAT
CAATGTTAACACTCACTGATTTAGTTCGGCGCTATCCTGCGATGGCGTCTTTGACGGATG
ACAGATTCGCCATCCTGCAAGAAGATTCCGTGCTAATCATGGGTACGGATGATGGACGCT
GGCTCAACTTCTATGACGCAGCACAATCTGCATTAATTGCGCATCGTGCAACCCAGTTTG
GGTTGGGTGATACTTTGGATGAAGATTCAGGCGGGATGTCTGCTGGGCCAGTCGTTCGAA
CAGATGTAGATGATGTTCAGGTGGAGTTTGCTGACCGTATTTGGGACAAAGTTCCTTATC
AGGAAGCAGACCTATTCTCAACGGCGTTTGGTCAAGATTATGTGCGTTGGCGTCGGATGG
CATTTGCTGGTCCGAGGTTCGCATGATTAATCAAAAGCTGGCGTTTAATCGTTTTACAAC
TGCAAAACACACCATCGAACTTTACATGGTTGGGCATTACGACGGAAAGAATAATTGGGT
TGGGGAAGGATATGCCCCACCTAAGAAGTTTCGTTGTACACCCATTCCATATGGTGACAG
GGATTCAGGCATTGGTGGCCAGAAATTGGTGGCTACTGATGTTGGTGAACGTCAACCAGC
ATTTATGCGAATTCACTCCAGAAGTCAAATGCCGATGAAATCAATAATCACTGTGTACGG
ACTCCGATACAAAGTGGTTGAAATTGATAAGTACGATGATGCAGGATTCAATGTCGTAAT
CGCTGCAAAAGTTCTGGAGAAATAATGGATATTCAAGAGCGCGAAATTCAGGCGATGAAA
GAAATCGTCGATGTGTGTGTGGGTATTCCATTGTTCACATACGAAATGCAATTGAATGCA
CCAAGGCCGGAAGGTCAATATGCTGCGGTCAAATGTATTTCAAGTTCTAATCCGGGCTAC
GATGAAACTCGCCTCATTGATGACAATGGTGTTGAGAAGTTTCGAACTCGTGGTATTAGA
ATCCTGACATTTTATATCCTCTTTAGTCGGGACGGTCAGGAGTATATTGATTTCGATAAT
TCATTTTATCGACCTGATGTTAATGCTAAATTGCGCGAACATGGTTTCGCAGCATTAGGC
AAAGAGGCGCTGAACCTCGCAACTGTTCAACTGGAAACAAACTGGGAAGTAAGGAAAGGC
ATTAAAATGCAATTCAATGTTCTTCGTGAACAACTTTCCGAGATTGGAACCATGTCCGAT
GCAGTTGTTGGTGGTAAGTTTGTTGATGGTGACCAAGTCATCACGATTAAAGGATAACAA
ATGTCCATTCCAATTTCCGATATCATCAATGTTAGCATTGCGGTTGCACCAAACGCAGTG
GCGACTGATGGTTACGGTCCAATCGTCTTTATGACGAAAGAGTTTCAACCTGTAATGGGT
GAGAATGCTCTGCGCATCTATACCAGCAAAAAAGCTTTGGATGAAGATTTCCCATCAGGT
GAAATCCAGAAAGCTGCAACTGCTTGGTACAGCCAGAAGCCTACACCTAAAACTTTCTGT
GTTGGCTCTATCAGTACAACTTCCGGTTCTCCAGCAACCTCCGGCAAACTTGTTGGTGGC
GGTGCTGCTGTTCTGGCAGATTTGAAAGCAATCACTGCGGGTGGAATGACCCTGAACATT
GATGGCGCCCCTCAGACAATCTCCGCTCTTGATTTGTCTGGTGCTGCCGATTTGGCTGCT
GTTGCAACTTTAGTTGAAGGTGCGTTAACAGGTGTTGCTGTAACTCAGGCGGCTGGTGTA
TTCACAATTACATCAATCACCTCTGGCATTGCTTCGGCTGTTTCAACTCCTGCTGCAACT
CCGTTGTCAACAGCTCTGAAACTGACGACTGCCACTGGTGCAACTTCAACTCCGGGTGTT
GCTTCAACCGGTATCACGAATGACCTTGCGAAAGCTTCACAAGCTGCTGCCAAGACAAAA
CAGTTCTTCTACTATGTTGCTGTAGATAAGTCTCTTCGTGAAACTCAACCTCTGCTGGAT
GCTGCTGCATGGTGTGAATCTGCTGGTAAAGTATTTGGTCTGGCTTCTGCCTCTCCAAAA
GTTCTGACCGCTGGTGACACTACCAACTATTTCTTCAAAGCTAAGTCTCAAAACCTTAGC
CGGACAATCTGCGTGTATGATGCCTCAAATGGCGGCGTGGAATATCCAGAAATTTCAATC
CTCGGTCGTGCTGCAACTGTCAACTTCAACGTTGCCAACTCTGCACTGATTCTGGCATTC
AAACAAGGTCCATCAATCACCACTGCTAACCTTGAACCAACTGAACTTGCTGCATTGCAG
TCTTACAACGGCAACGCATTCATTGATGTTGGCGGAAACACCATGTTCTACAATGGTGCG
ATGGCAGATGGCACTTGGTTTGATACAATTCAGGGCGTCGATTGGATGACTCAGAAAGTT
CAGGTGAATGTGTTCAACCTGTTCTATCAGTCAACAACCAAGGTTCCTTGGACTGAAACC
GGCGTAGCAATGGTTAACCAGCAAATCACTCTGGCTCTTGAGCTGGCTGTGACCAATGGT
TTAATCGCCCCAGGTTACGACAACGAAGGCGTGTTCTATCCAGACGGCTACAAAGTTTTC
TCAACCGATTTGGCTCTGTTGCAATCTCAGAAAGGCAAACGTATTTGGGAAGGTTCTTCC
TTTATTGCGATTGGTTCTGGTGCGCTGCAAGGTGCGGTTATCTCTGGCAACTTCGTACAG
TAAGGATGTGCTAAGTGAAGCAATATTCATTCTATAATACTGACCTGCTGATTGATGGTG
CCCGTGTTGACGGGTTCACCGCTGGTCAGGCAGTTATCACTGCACGTCGAAATGTACCTC
AGCATCTTCCAGTAATGGACGCATATGGCAAACTTGCTGTTGCGACAACTGCTGACTTGT
CTGGTGTGATTACATTCCCACTCCTGCAAACCGCGGATTGGAATGAGATTCTGTACCAGC
GTGCTCAATTGACTCAGGCAACTGGTTTGTCTGGCAATAAATCTCTGTGGCAACCAATTC
AGATTCAGGTTGTCGATAAGATGGGCGACATGCTTGTTAACGGCGTCAATGGTGTAATCC
TCCAACAGCCTGCAATCGTTCGTGGTATTTCCTTTACCTCAAACCTGTGGGCAATCTACG
TCGAACGTCTCCAGATTAAGACTGGTTCTTATCCAGAGATTGGCGTTTAAGCTATAAGAG
AGAAACAGAATGGCTTGTGAATTACTCACGCGAGAGTTTAAGACTTCGGAAGGAGTTACC
AAACTCTTCACGACCCGCCAACTGGCGGCGAGTAGTGCACTGGACTTGCATGTTGAACTC
ATTGGTAAAGTGGGAACCACTGTGTTCCCACTCATTGACAACAACTACAATTTCGGTGAC
ATCATTGCAGTAATGCGTCAGGCAGATAACAAAGTTGTTACTGACTTGATGAAGCGTGTA
ATCTGTAATGCAAACATTGAAGGCCAGCCAATCACTATGGCGACTTTCGATATCCATTTC
AATGGCGAGCTCATGTTAGCTTGTCAAGTATTTGCCTTCGTGCTTGAAGCAAACTATAAA
GCTTTTTTCAAGCTAGGGCTCGAAATGAACGAACAGTTCAAATTGGCGGAGGCGGAGCGT
TTGGAAGCGGAACGGTTGGCTATGGAAGCGAGCCAGAAGAATACGACTCCGACGACTTAA
TCAGTCAATTCCCGGACATTCGGTATTTTCTGCATCGCCCAATTATTGAAGACAGTTCTT
TATGCAGACTCCACGAGTTGCAAGATGGAACCTATTCATTAATCGACCTGCTCCTATTCC
ACGACATTTTAGATTTAAGAGTTAAATTAAATCCTCCAGTTGAGCGGGAACAGTAATGGC
TAGCAGAAAAGAAATGATGGGCATGGGCGTGGATGTTCTCCGCGCTCTTGGTCTTGAGGC
CGGATTGAATTTTGAGCCCGGACTCTCGAAGTCCAAAATGGTTTTACAGCTGGAACAGCA
TCAGGCTTCCGGTTGGATGGACGTCAATAGCCAATTACTACACGCAAGTGCAGAAGGTGG
CTTTGACCACATGCAAGGCTTCACCGGTTATGAAGAAGAATCCATTTCCGATGCAGCGCG
TGTTGCACAGTCATTAGCTGGTGCAGGATATACCGAAACTTTCCATCATATTATGTCTGG
TGGTAAGTCTCATCATGTAGAAGCTGTTCATGGCTACATGGCAAAACTTGGTGTTTCAGC
CGATGATGTTTGGCTGCATATGCCAAAACCAAATCCTGAATTACCTGCGCAAGATTTCAA
TTTATTAAAAGGTTATTTAAATAACCATTGGGATGATTACCAAGACATTATGCCACCACT
TGCTGGGCATTATGCTGGTGATATTATGGGTGAGTATGCTACGAATAAAGGCCATGTTGG
CGATTCGTACAACCACCTTTCATCCATGTATTTAAACAAAGCTGCTTACAACAATCCACA
CGCATATGAGAAAGATGCTGCCCTTGTTGCAACGCGTTTGGCTTCGGCGATGGGAGGTCA
ATTCCTTGAAGTTGGTTATGCTGCTGCCACTGGTGCAAAGGTTGGGTATCATTCCATCTT
GCCTCAGCTTGGTTCTGATATTGTGCGTGGTGTTCAACATCCTCGCGAAGCTCTCAATGC
TGCTGGTTTGCCTCTTGGCTCAATGGGCTCTGCTGTTGGTAGTGGTCGTGGCGATTATTC
TCTTGTCGCTTCTCTGCTGGGACGTGCGGAAGGACAGGATGAAGCGTCAAAAGCAATCCG
AAGTGAAGTAAGTAGTACGCTGAAAAGTTTGGCAGGCTCATATAAAGGTGGTGGCGAGTT
TGGAATGAATCCACATCGTCATGAAACATCTGAGCATGACTTACTTCTCGATTCTGCAAC
TCGTTATGTTGGTATTGAAGATGCACGTTCTGGTTATGCAAACCTTGACGACACTTCATA
CAACAGAAATAACATTGCAGCAATAATTGAAAACAACTATGACCGCATGGATGCTCAAGC
TGCACAAGCTCCATCTGGTGTTGCCAATCCTGATTTAAGAATGCCACGTCGTTCTGAGAT
TGGCACTGCATGGTCTGCTGATTTAAATGAACCAACCGACTGGAATGCTGCTCGTGTTCG
TCGTGAAAGTGCTGCCATTGCAAACTTGGATGCTGCTCATGCAAACTTCCGTGATGTTGC
TGATGGTGGTCCAAGTGGTTCAACTGTTCGATTCCATAATTTCCAACAAGGTTCTCAGGA
ATGGCACGACTTCCGTAGTCAATATGACATAACTGGTTCCACTGTTGGCTCGATGTTGGG
TAGTAATTCATATACTCGACCTTGGGCAGAAATGATTGACCGCATTGGATTGCAGCGCGG
TGATGGAAGTAAGAACGCATTCACAGAAAGAATGTTTGCGATGGGCCACAAGCGAGAAGA
TGAAGCTCGTATTCGTGTCTCAGAAAGATTAGGTCAAGAAATCCAACAAGTTGGCTCAAT
CACCAATGACCAGTATCCGGGAATGATGTATTCTCCAGATGGTTTAATTGGCGATGACGC
ATTGTGGGAGCATAAAGCTCCTGAACGTGCTGGCAAGTTTGCAGATTTGCTTGCAGGTGA
CCATCCAGACTACATGGACCAGATTCAAATGGGCATGTTGGTTTCTGGAAGGAACCGAAC
TTTATTCTCGCAGACAATTGGAAGTGAAACTCGCGACCAATGGATTGACCAAGACCCAAG
TTGGTATGGCAGAAACAAAACTCGACTCGATTCTATTCGTGGTCGTCTTGCTGCCGGTCG
TGAATTTGTTGCAAGCAATCCAGACCTTGACCAAGAAGCGTTAATCAAAGGTGCTCGTTC
TGTAATGCAGGGCGAAGGCATCTGGCGTGATGTTTCTCAACGTTCCAATCGTGGTTTCTC
TGCAACTGCTGGTACTGACGCTGACCCATTCATTGGGCGCGGCGGTTCAACCTACAATGA
CGCTGCTCAACCGTCTTCAAACTATATTCCGAACTTTGTGACCAGTGCCGGTAATGGCCT
TGTGCCAGTGGGCGGAAATGGGGCTGAGACGGGCATGGCGCTTGCCGTCAAAGAAGGCAT
ACTAGGTGCTCAGGAGGAGCTAAAACAGCGCCACGCTCAGGCTGGTGCAGCCTCTGGCGG
TGTTGCTGGCTTGCTTGGCTCTGATATGCCAGACGCCGATTTTGCCGACTCAACTGGCTC
CGGTGGTATTCGTGGATTCTTCCAACGAATGTTTGGTGGTGGCTCAGGCGGCGCAGGCGG
CAACGGTGGCGGCTCTGGCGGTGCAAGTGGGTGGATGGATGACTTTGGCGGCTCGCTCTA
TGGTGGCCTCCGTAGCGGCAATTTGCGTGGGTTGCAAAATGGCACATTAGATGCAATCGA
AAGTCTGCCGATGGTTGGCGGAATTCTTCGAGGAACTATTGGAGCTATGCAAGCTGGTGG
TGAAGGCATTGCAACAATGTCAAACTACCGTGGTATTGCAGAAGATTCAGGCATGACTTC
TGGTGTTGCATTTGCAGCACAGACTCAGGGTTTGGAAATGATGGGCTTGAATGCAAATCA
AGCCGGTGCAATAAATCAAACAATTCATTCTGTATACAACCGTATGCAAAATGGAGACCC
TGATGCTGCTGTTCAAATTGCAGTGCAAACCCGTGGATTGATTACTCCTGCCGACCTTCA
AAGTTCTGGTGGTGATGCAACCAAGATAGCTGCACTTATTCGTCAACGTGGCGAAGAACG
TGGCTGGAGTCAGTCTCGAATTGCTGGCGCTATGGAAATGGCTGGCCTTGATGGTATGGC
TCGTGTTGCTACCAGAAGTGAAGCAACGATGGACCAATCTCGTCGCCTGGTTGATGCTCG
TTCTGCTGAAGATGTTTCTGGTGCAAATTCCAATTGGGAAAATGCACAACGCAATCGTGC
TCTTGCTTCCACAGATTATTCTGTTCCTCGTTATTCATTCGAAAGTTTGGGTGGTGCTGG
TGAGAGTTTATCTGGAAGTATGGTGCAGGCTGCGGCGGGTGCTGAACGTGCCTTCCGTGC
TGGCGGAGACATGATTGACTTGGTTCGCAATCTGGAATCAGGTGGTCAAGACTTCGATGC
TCATGGTAATCCAGTTACTTCATCGTCTGGTGCGAAGTACTCAATGCAGGTTCTTCCTTC
TACAGCCCGTGACCCAGGTTACGGCATTCGTCCAGCGGCAAATGATTCTCCAGAAGAATA
TAACCGTGTTGGTCGTGAGAAGTTAGATGCAATGATGAAACGTTACGGTGGCGATTGGCG
TAAAGCTAATGCTGCATACACCGATGGCGAAGGAACTGTTGATAGTGCAATCAAGCAATA
TGGAAACGATTGGTTGAGTCATATGCCGTCTCAAGCACAGAAACGTGTAAGAGACATGGA
ACGTGCTGGATTCAATGGTGGTAACGCAAATTCATTCAGGGATGCTGGCTCAAATGGTCA
ACAGAATCTGAATGTAACTGTGAACATCAATGCAACTGTTAATGACAAAAGCACTCGTGC
TTCTGTTACTAACCCGTACAGTGGTGAAGTTGTTACAATCAACTCAAATCACGGTTCTGT
TGCACAGCGTCGATGAGATTTGTGCGGGGCTTCGGCTCCGCGCTTTCTTAAACTTTATTT
GAAATTGATTTATGAAGGTGTCATTGTGTGGATTAGACAAGTACAGGTTATCGTTACAAA
CGCACAGAATCCAAAAATAAAAACTGTATTTGAAAAACACAGTATTGAATTTGAGGTGCG
TTCGACTTTAGGTTGGGCTGCTGATACAGCAACAGTAACAATCAAGAACTTGTCTTTGCC
AGAAATTAAATTCTTGCAGAATAAAACCTTTGGCGACCTCTTGATTGAAATACGTGCTGG
ATGGCTTGATGAGTTGCAAGGCTCAAGTGTGAAACAACAATGGGTTGCACAAGGGCAAAA
TGCTCTGGTGACAATAGAAGAGAATAAAAATCTTCCAACGTTGTTCTCTGGCATTCTGTC
AAATGCTGTGGGGTATAAGAAAGCTCCCGAGCATATAACAACTTTATTCTGTGTATCAAA
CGCAGGTGCAGTTGCTACATCGTTTAAGCAAATGCGGGATATTCCTGCGGGTTCAACATT
GAACGATGCAATCAAGTCTATGTGTACTGATTATGGATATAATACCGTTTCCTCTTTTGG
TGTGTCTGGAACAACACTCACAACAAAACTTCCTATGGGACGTGTATTCCATGATACCTT
CATAAAAGAATTTGGTAATTTGCTCGAAGAATACAATTTAAATTTCTATATCGCCACATC
TGAGATTCAAATATTCTCAGACACGTATGGTGATTCTGATGCCATGAAGCGAATGGCTAA
AGATAGAGAGCCAGTTAAAATTGATGCCAACTCAGTTATTGGAACTCCAATAGCGGGCAT
TGGTATTTTCGATTTGGCAATGTATCTTCGAAGCGATATTCAGCCCGGAATGGTTATGGA
TGTTTCTCCATTACTCGGAACGGAATTGCTGGCAAATGGTGTAACAAGTGTGGCCGGTCG
TGGTCAGGCACTGAACCGTGTTGACTCTTTATTCCAGTACGCAATGGAAGACAAGTATCA
AATCATAACGGTGATACACAGAGGTTTCACACATGACCAAGACTTCCAAACCATAATTCA
TGGTGTTCTCGGTGGAAACACTGCATCAGGTTTGTCAGAGCAAAACTGGCAGAACTGGTA
CATTGGAAGTGGCATGGCGTCAGCACACTAGAGGTTCTAATGTCAGATATAATTGGTGAT
GTTGGCACAGCTTTGAGCGGTGCTGCATCCAAATTAAAAAGCAAGTTCAGTTACGGACCG
CATCCATCAATCATCATGTGGGCGGCTGGTCCTGCAACTCAAGTTCAGGATAAAGATGAT
TCTATTCTTGGTTCTATTGGCGAAGCTATTTCGGGTGCCAGAACCAAGCTGGAGAACATC
GTAACATCTACACCAGACCCAAGTCAGTTTAATTCATTTAAATTTGATGCGATGGTTAGT
GAGTCTCATCTTGCTCAATCAACGGTGACAAAGGTTCCTGTGTCTACCGGGTTTGTTGTG
TCTGAACACATCATCAATCATAACCGCATTCTCAAACTTGAAGCAGTTGCAACAAACATG
CAGAACTCTGCAATGTGGGCTGTGTCTGCTCAAGGTTTATCTGTGGTTAGTGGTGCAATC
TTCAACAATCCAATCCTCCCTCAAATTGGTGGATTGTATGGTGTGGTATCTTCTGCTTTT
GAAACAGAAAACAGAATTGAATCCACCTACAATTTATTTAATGGGTTAAGGACTTCCGGC
ACCAAGTTGTATATATCAACCATTCTTGGAAAGTATTTGAACTGTGTAATAACAGAAATA
CGAACCAAGCATGACAAGCTCACTGCCTCAATGCTGGCGGTTGAGATTACGCTGGAAGAG
TTGCAAACAATTGGTAAGGATGACATTGCTAAAGCTGCGGCTACTGCAATCGACAACTCC
TACGATTATTCTGAGTTTGCCAAAGTTGCAACAGGGCTTGGTGTTGGTGTACTTGGTGGA
GTTCCTTTACCGGGACTTGGTTCCTTGTCGAACCCAACTGCACAGCTCGGTGTTTTGAAG
GACAAGCTTGCTAAATTGTCTCAACCTATCTCAGCAATCAAGGGAAGGATATTATGACAG
TAAAGGAAGAACAACAAGCCTTCAACGAAATCATGAGTTTGTTGCCTCCTGGGTATGTTA
AGAAAATTCCTACATCTGTGACAAAGGATATTTCATTCCAGTTTAATGGTTTGAGTATTT
CAATGTCTGCACTGTATCTCAATACTTTTCTTAATTGTTATTGTTTTGATTTGGCATGGT
CTTCCACGGATAAAATCTATGGCATACCTCTCAGATGTGGTGTTGATATTTTAAAACAAT
ACTCGACACCACTTCCAAATCTTTATGCGAACAATCATGCGTATCCCGGAAGCGAAGTGA
CGAGTTGGAGAAATCTCGACTTAATCATTATTGACGAATCGGTGCTTGAACGTGGGTAGT
CAAAATAACACTCGTGCTCCAGATATAAATACAGGTTATCCGGGATTTGCTTACAACTTT
GATGCCGACACACAAACCTGTGAAGTGCAACTTGCAATTGAAAATTTGTTTATCGGAATG
CAACAGCCATATGCTCTTGTTCCAAAAAGCAGATTAAAGAATGTAACGGTTCAATTCACG
CAAGGTGGTGGTTGGAGTTTAACGCATCCGGTTCCTGACGGTACGCCAGTGTGGGTTCAT
TTCGCTCAGCGAGGAATTGCACACTGGCAAGTCGAAGGCAAAGACAGTGCAGGATTAATA
AATGGAAAACCTGCTCCTGCATTTAGCCAGTTGTATTCAATCAACGCGGCGTCATGTACT
GTCGGGAACCAACCTATTCCGAAAGCAATAAAAGCTTTTAATAATGGTGTTGCTGAGTTT
CGAAATGCTGATAGAAGTCAGCGTGTAACTTTGAATGAAAGTGGTGGCATTAACATTGTC
GCCAATTCAGCAAAAATCATTGTTAATAAGGATGGTACGATTGAGCTCGACTCTCCGACA
GAAGCGATTGTTAAGGCACCGAAGATTACATTGGATGGCGACACAACAGTCACCAAATCC
CTCACCGTTCAAGGTGGAGCATCAATCTCCGGTGGTACAGGTCAAACCTTCAAAGTAACC
GGTAATGTTGAGCATCTTGGTTCTTATTCTCTGAATGGAATCAAAGTAGATGGTCATAAC
CACACTAACCCGGAAGGCGGAAACGTCGGCCCAATGAAGAATGGATAAATGTCCGGAAAC
CTAAAGCTAGATGCAAACCACGATATCATAATTGGTCGTGGAACAACTCGCGTTGGTGGG
GTTGAATATGTCGCACAATTGGTTAAATGCCGGTTGCTGACATTGCTTGGCGAATGGCAA
CAAGACAGCACTCTCGGACTTCCTTGGTATGAAGTAATATTTACTAAAGATGTTCGGGAT
TCTGATATTCAAGTTGCTGTAGCAAATATAATTCGCTCCACCAACCATGTGCGTCAACTT
CTTTCGATAGATATTGATGCAGATAGGAAAGCCCGAATCTTAACAATTAACTTTGTAGCC
ATTTCTGATTATGGCAATATCTCAGAGGTTGTGGAATGGCGACAGTAACCTATGGTGTAA
CGGATAAGGGATTTGTCCGCAAGCCGCTTGAAGCAATCTTGGATGATTTGAACTCTGCGT
TCAAAGGCGCTTACGGCTCAACTTTCGATACTTCACCAGAATCACCAGACGGTCAAGCTA
TTGGCATTATTGCTGATAGAATTTCTCAATGTTGGGAACAGGCACAATATAGTTTCAATG
CCTATCGTCCCGGTGCTGTTGAAGGAACTGGTTTAGATAATGTGTGTGAATTAACCAACA
CAATAAGGTATGTAAACAAACCAACTCAGGTCACTGTAATGTGCACTGGTGCAAATGGTT
CTGTGGTGCCTGCTGGACTAGTTGTTGGGGACGACTCAGGTCACACATTTAAAACCAAAA
TTGATGTAACCCTTCCGGGAGACGTAACAGCATATTGCGAGCAACAGGGTGAAATCTATG
TTGCAGCAAACACTGTTACTAAAATTGTCTCTGGCGGAAATGTTTCACTGACTTCTGTGA
CAAACCCGGAAGAAGGTCAGACTGGTATTGACTATGAGAAAGACCCAGCACTCCGTTCTC
GCCGTGACAAAACAACGGTTGGTTCAGGAACTGCGACAGCGGAAGCAATATATTCAGCAC
TCGCTGACCTCAATCTTGATTACATTCGTATTCGTGATAATGATACCGATGTTGCTATTG
GTTCTCAGCCAGCAAATACTATTTGGGTGGTAGTTGATGGTGGCACGGTGAACGATATTG
CTCGCCGCATCTACGACAACAAAGCTGGTGGTGTTCCTACCTATGGTGATGTTGTTTCAA
CATTCAAGGACAGTAAGGGTTATCCAAAGACAATCAAGTTTAGCCGCACTTCCAAAGTTG
CGACTTTCTTTGATATTAAAGTCAAAAGATTGCCTACTGCTAACTTGAGTTCTAACGATG
TTGTTGTTGCTGTTCAGGATGCTGTTCAGAATTACATTGACAGTTTGCAGCCCGGTGCTC
CAGTTGTTTGGTCTTATCTGATTCCGCCAATTCTTGCGTCTGTTCAAGGCATTCAGGTAG
ATGACTTGCAGGTTGGATTAACTGCTGCGAATGTTGCACATACAACGCTCGAAATGGATA
TCAACCAGCGTGCCAGCACTGCAACCGCGAACATCAAAGTTACGGACACCACAGTTCCAT
AAGGATTAAGATGGCCGAAGTTAAACATGGCATGGACTTGTTACTTTCTCAGTACAAGAA
CAGCCCAAACCTCAAGAAGTATATTCAGTGCTTCTTAGATGAACTTGATGTTGTTTCATC
GGCTATGTCTGATTCTGTTAAGTATAGATATCTTGCAGACTCCTTTGGGGTTATGGTTGA
TGATATTGCTTACATAGTTGGTGCAAGCCGTACTTTGTATGGTGCGGCTGCACTTGGGTT
CTTTGGTTACTATGAGAACCCTGCTGCGGAATCAACTGGTGATGATAACAACCCTCTTGT
TGGTGGTATTCTGCGTTCCGATTCTGACCGGAGTTCTGGCGATTTTGTTCGCACTGATGC
TCAGCTGAAGAATGCCATTAGAGCACGCATTGTTAAGATTGTATCCAATTGCTGCATTGA
AGACTTAATTGTATTTTGTGATTTGGTCTTGGGTAGGGAATTGGATATGGAGATTGTGGA
AGGCCCGTTGAAGCTGGAATTCAAAGTTCATGAAACTTTACCAACTGCCGACCGTGTTCT
TTTGTCTTTCATGATTCCTGATATAAAACCTGCTGGCATTACAGCCAAGCTGGAAGATGA
TTCAGGCAACATTGCGTTAGTCTACTATTCAAAAGATTATCCAGCGAGAGTGATATGACA
AATCGAGTTGGTGATTTAAGTCAGATTTGGGCATCTAACGGGGAAGCAACAGACCCCGAT
TTAGATACCACGCATCCGATATTCCAGCCCAATAAATATATTAAAGGCTGGAAGGTAGAG
AAGGAGCCTCACCAATGGCAAAACTTCTTGTACCAAATCTCTGATGAAAAATTGTTGTTG
GTTGCTCAGGAACAAATCTTGGAGTGGGAATCTGATATTGGCTATGCCAAAAATGGTATC
ACCAGAAAAGGAGACAAACTCTACAAGAATGTTAGTGGTGCAGTTTCCACTGGTAAAGAC
CCAGCCACAGAAACATCTGTGTGGAACGAAGTTCTGAGCATGGACTCTGCTGGTGTTGTT
ACAGCCGTAACCACTCTGACAAACACACTGAATGCTCACTTGGCTGCCGACAATCCTCAC
AAAGATAACATCCATGATATTGGTGGATATGAGTCTGGTGAGATTGATAAATTCTTTGGC
GACCCGAACAACCCTGCAACAATCGTTTACCACGAAAGTCAGACAGGAGGTAAAGTTCAT
GGCGAAACTCCAGCGCAAGTTGGTACCTTGCCTACCAGTGGCGGAGCCTTTACTGGTGAT
GTTAATTACATTGGTGGTTTAACAATAGGTACGGGAAGTTTCCGAATTGATGGACCAGAC
CAGAAGATTACAAATTCCGGTGGTTCGCTATTCCTTGAAGGTGATGGCGATACAACTGTT
GGTGTTGAGTCTTCTGGTGTTCGCAGTGATATAACAACTGTTGCGAACTTCGATGCACAT
CAAATTGCAGTAAATAATTCCTTTGCACTTCCTCAACCGATTCTGCAAATGCAGCTTCGT
GCTGGTTCTTTTGACACAATGGGTGTCGGTAACTTCATTGTTGAAACAACCGATGCTCCA
GTGTTCGAAGGGAATAAGGGATGGAAGATTGCCGATAATACATTCATGACTTGGGTGGGA
TTAGTTAGTATTCCTGCAACAATTTACTTTGAATACTATGCTGGCGATATCAGGAAGTTT
GCTGTTGTTGATGCCACACAAGTTGTCGTCAAACCAAATCTGAAAATATTCCTGCAAGCA
TTGATTCCTGATGCAACGCATTTGTGTGTTATGGATATTTATCTTGCACTGACTGAATAC
CAGAAGTTGAACCTGAAACCGAAGTATGATTCTTTGCATATTGCAAATGATACATCTTCA
ACTCCGGGAACAGCTTGGTATGTTTCCAAAGTCACGACAGATGTTTTCGTAATGCCTAAT
GCAACTGCTCCCAAAAATGGTCAGTATATTGGTGCATTCCGTTGGAGTCCTGACGATGTT
CGTGGTGCTCGATTGGATGGCAGTGCTGTGTGGACAACTTCCGATTCAAGTGTTGTTCAG
ATTGTTGGTTCAACACAAGCAAATGGTCAGGCTGTAATCAACATCCTGAAAGCAGGAACT
GCAACCTTAACTGTTAAATGGAACGGAATGGCTGCAATTTTAACTATCACTGCTGTATAG
GAATAGATAATGCTGCGTCCTAAACTTGGCCGAATTTGGGGTGACTCGAATTCGGTTGCT
CGTCGTGACCCAGGCGACGAAAAATATTTAAAAGGTTGGCTGTCTGAAATCCCAACATAC
CAAGTTCTGAACTATCTTCAATGGAAGACGGATTCAACACTGCAAGCTCTTACTGAACGT
GGTGTGTTTGAATGGGGTGGCGATGCAACCTACCGAAATGGTGCTGCGGTTTGGGATGAA
TCAAATGGAGTGATTTATATTGCCAAACTTGATGGTCCAAGCAAGACGCTGAAACCAAGT
TCAAACTTGGCTCAATGGACTCCAAGTGCATTGCAGATTACTCGTGCAGATTATGATGCA
ATTTCTGCGGCAATCGCAAGTCACATCGCTGATGTAACAAGTAACCCTCATGGACTGACT
CCGGGAAGACTTGGAACATACACCACTGCACAAATTGATGCATTGGTTGCTCAGTACAAG
AAAATGGTTTCGGACCATGCAGCAAGGAAAGATAACCCACATGGAGTTACAGCCACTGAC
ATTGGCGCAGTTCCTGTAACTGGTGGTGCATACACCGGCAATGTTGAAATGAAGACTGGT
CAAGTTCTTCTGTCTGCCGATGGTGTACGTAAAATCAAAGCTGATGACACTGGCGTCTAT
ATGGCGAACAGTGCAGGTCAAGTTGGTGTTGATGCTTCTGGTGAAGGTTTTGTCAAAACT
GGGAATGCTCCAGCAACCAAAATCATTACTAAAGCAACATTTGCAGATAATAAAGCTGCT
GTTGAAGCAGCCTATGCAGTGCAGCAACCTGTGTTCTATTTGCCTCTTGCATCTGATTTG
AATGTCTACATTGGCGGTGGTACTGTTCAGGCCGACCAAAATGGTGGTGCACTGGATTGG
AATCCATCTCTACTGAATGGTGTAATCTTTGGAAACAATACACAATATGACAACCGTGTT
GTGTATACATACAAACCTCTTGAAGGTTTGAAAGAAGCAACCATTTGTGTTGATGTTTTC
CTTGAAGGAACTGGCGGAACTACAACTGCTCCTGAATTGTATGCATCATTTGGTGCTGCT
GCTGGTTGTGGTGTTATTCAAGTTAATAAGAATAACACAGTTCGTTTCTGGTCTGAGACT
GGAACAACTGGAACAATCCCACTTCCAACCAGTGTCTGGCTTCGTCTTGTTGGTGTAAGA
ACTACAACTTCTTTGTCGCTGTATCTCAACGGAGTTCTGGTTAGTACAGTTGCAGCCAAC
AATGCAAACATCACAGGAAGTGATTACAACTTCCTCCGTGCAGATGTTCAGGCACAAGCA
AACCAGAGACTGATTAAAGCCAAAAACTTTAAACTCTGGATTGGTGCTTTGACGGCAAAA
CAAATCTCAACGCTATAAGGGTTAAAAATGGCCAAAGGTTGGTCTTTCGGCGCACTTACA
GCTGCGTTAAAAGGTGCTGCATTTCTTGATGTTGGCACCACTGCAAACACTGTCGCTGCT
GGTAATGACTCACGAATTGTCAATGCTTTGCAGAAGAACAACAACTTGACTGATGTTGCC
GACAGGACTGTTGCAAGGGGTAGTTTGTATGCTGCCTACATAAACGGCGATGCAAGTCAA
GCATTCCAGTGTTCGAACAGTGGTTCTGATAACTATGCTGTAAACAATGTTCGACTCAAC
TTCATGCTGCAAAGTAAGGCTGCTGTAAATGGCAACCAGTACACACAGTTTGATGTTGGT
GGTGCAACAAGTGGTGCACATGCTGTACGTCTAGACCAATTCCAGTCTGGTACAAACGGT
AATGGTGCTTGGACTAAGATTCCAAATGGTCCTATGTGGTGTAGACAGAACTTGTCTCTT
GCAGCAAATGCAACCACAACTTGGAATTTCCCGGCGCAATTCCCTGGAAGTCCTGCTGTA
TTCTTCTCAGCTTTCAATGGTGAGAACAGGGCGTGGTTTAACGGCATTTCTCAAAATGGT
TGTAACATCTACAATCCACAGAATGTCGCGGTCAACGTTAACGTGTATGCGATATGGTGA
TTGATAAGGGCTACAAAGTGGATAGACAAACTATCCAAGTAATCTTCCTATTCATGCAAC
AGTATGGATTACTGCATGGTCTGTTAGCTGGACTTACTGCTTTAATTCGTGGAGCCTATG
AGAGTGAAGGTCTTGGAAAAGCTCTGCTTGATGCAGCGCTGTGTTGTGTTATTGGAACGT
TTGTCTTCTCACTCCCAATTCTGGATGGTTATTTGGTTAATCATCCTAATGCAGGATTTG
TTGTTTGCATTGTGATTGGTGTAGTAGGTGCGAAGTTAATTATCACTACTTTACGTGATT
CGTTCTCGGCAGCAATTAAGCAATTGAATCCATTAACTTGGTTCAGAAAAGCAAGATAAA
AGGAACACAGATGCGGAAGTTGTTTTGCATCATGCTCCTGATATTTGCGTGGAACACTTC
GGCATTGGCAACTGCTGATGCTGGAGTGAATTCCAAATGGAATGCGCATGTGTCGGAGTT
TGCAGCTCAATCTTATCTCCAAGGTGGATTGAAATTAACTGCAAGTCAGAAACATGAAGT
TGACTGTTTGGCTAAAGCTATCTTCTTCGAAGCCAGAGGAGAAAGCTATGCTGGAAAGGT
CATGGTTGCAAATGTCGTACTGAACAGAGCTAAGTTTGGTAAGCCATTTTCAAACACCAT
CTGTGGTGTAATTTATCAGCCACGCCAGTTTTCATGGACTTCAAACAAATGGAAGCGGAA
CACTTCTTTTAGAGAAGTAGCGCGGAAATTTGACTCAAAAGAGCACAAAGCTGTGGTAGA
AAGTCTTGAAATTGCATTGAAATTTGTGATTTTAGACACGAAAAACACAGGAAAATGTAC
TCATTTTTCATCAAAATCTGACAAATTTGGACGTACTTTGTTCATAAAACAGGTCGGAAA
TCACAAATTTTATCAATATTTAGGTAACGGATAATGGATAAAGCTCTAATGCAATACTTG
GCAAATCTTGCCGAAGAACACCATTGCACTATTATCTTCCTTGATAAATCAATCTGGACT
CCTGAAAAGTTGATTCAAATGCAGGAAGCCTTGCTCAAGATTATCGACGAAGATGCCGAA
ATTAAGGTGCATTGAATCTTGTCAGTTAGATGTTGAGGGAAGGTGGTCGCGAAAGCGAAC
ATCCTTTCCGAGACTATTAAGAAAGAAATAAGTTAACAATATTCAATTCCAAATATTGCT
ACAATATTATGCATGTGATATTTGTAATGGGATATGCGATGGATGGTCGGTCAGCTGTAC
TTCATCTAGCTGGCTTAAATGGTCAGTCGGCTGTACCAAATTAAATTCGATTCGAGATTC
GCTTATGATTCGAAATTTAGATTCGGATTAAGATTCAAATTTTAATATTAATTTTTTTTT
ATTGGAGCCAATGGTGCCGGATTCGAATACGAATTTTATAAAAAATTTTTTTAAGACGTA
GACCGGGAGCCCAACACCGTCCTATCGGACTTAAAATACGGTCCCTTAACTTCTTTTTCC
AATTCGTCCTATCGGACTCAAATCAACACCAAAGTAGCTCCTGCTTCGCAGGCATAACAA
CGCATCTACATCAGCTCCCTTAGGTATCAATAGTGTAATGGCTACGCAAACTAAATCCGT
TTCATCCGACAACGGCACTGGTTTGTGGGCTTAGTTGACTATGTATAAAACCTTGGAGCT
ATATCATGACTAATTCAAACGTTGTTAAATCTGTTGTTCCTTCTATCACTTCTGCTATCT
TCCGTGCTAAAGAAACACAGAGCTACATGCAGCCACAGACTTGTGCTATTGCCGCTCTCG
CTATCTTCCACATGATGACGTCATTCACACGTGACGATGTTGTACCAGCCGTTGCGATGG
TTAGCCTTGCAGAAGCGTCAATCAAGAAAGCCAACTCTACCAACAATGACCTGAAACAGT
TCCACTATGTTGACCCATCTATCAACTTGATTGGACTGGCTAAGCATCTTGGCTATGTAT
CAATTGCAGAAGATAAGTCATTCACCATGACTGAGCGTTGGATTGAACTCGTTTCAACTA
AAGAGACTTGCACTCCACTGACAGAAGCTGTGTCAGAACTCAATCGTCGCAAGCCATTCA
TCAAAGGTGGCAAACGTAAGCCTTCCAAACTGATGACAGAATGTATCGACTTCCTGCAAT
CAACTGAATACCATGTTGATGCAGCCATGGTTGACATTGTTGGTGACGTACTCGGCATGT
CTCATGGTGACATTCCTGATGTTATCAAACAAGAGTTGCATGTTTGGAACAACGCTAAGA
ACATGGCGAAAGAAGATGTTCTGTTCTCTGATTACTTTGCAGATAACCGTGGTCGTTTGT
ATCACGTTGCATGTGCTGGTCCAAATCCACAGTCTTCTGACTTTGCTCGTTCACTCTATT
CTCACAACATCGAGAACATTGTGAAGAAAGACAGTGCAGCATACCAGATGTTCATGGCTG
AATTGAATGACATTTCTGGTGGCAAGTGGACAGAAGCTCGCATGTTAACTCGTGTTGCTC
AGAACCCAGTTGGAGCTCTTGCTCACATGTTACGTTCTGGTGACGCTCCTAAGAAGCCAT
TCACTTACATTCGTCTGGCATTGGATTGGTTCAAGTTTGAAACAACTGGTGAGTGTGATT
CACGTGTTGGCTTTGGCTTAGATGCAAAATGTTCTGGTACTCAGTATCTGGCATTCATTG
CAGGCAACATGCAAATGGCACAGGCAACTGGATTAGTTGGCTCAGATTCTAAAGCAGCTG
ACCCATATCAATTGTCGCTGGTGGAATTGTTGAAACTGTTAGACAAGTCTTCAATGAAAC
CTACCGATGAAGTAAAAGAGAAATATTTCAATCGCAAAGATGGACGTAACTTCATCAAGA
CTCCATACATGGCTGTTCAATATGGTGGTGGCAAGAAAGCTCTGACAGGTAACAAAGACT
TCGTAGCAACTGTTACTGAAATCTTTGGTGCAGATAAAGTTGAAGCATTTGCTGAACTTG
CTGTTGAAGCTGTGAAAGCTGCACTTGGTGAAAAGATTAACATGTTCATCGCTAAAGTGA
TGGAAGCTGTTAAAGCCAAATGTGAGAAAGAATCTAAACTCTACATTGATTATCGCCACA
CTGATGGACAAGTGGTTCACAAACCTTGCTACCCATCACGTGAAATCTGTGACCCATTCT
CGATTCGTGTTGACTCTACAACTCGTGTCATCTTTGGACAACAGAATGAAGGCAAAGCTT
GGAGCATTCGTGAGACACAGCCAACTGTAGAAGAATTTGTTCGTACCTTTGTTGTTAACT
ACATTCAAGGTATTGATGCATTGGTTGCACGTACTGTTGCCAAGTATGCAAAGAAAGCTG
GACTTCGTGGTTTCACTTCAATTCACGATTGCTTCCGTTGCTGCTTGGCAGATGCTCCTA
AGATGATGGATGTAATTCGTCAGGCATATGTTGAAATCTTTGTGAAGAATAATCAATTCG
AAGCATTGAGCAAACAAATTGGTGGCATTGATATGTTCCATACCAATATCGTAACAGAAG
AACTCCTGATGAGCGAACACGCTTATTACTTCTGCCAATAATCCAAAACAACTAAGGGCT
TCTTCGGAGGCCCTTTCTTTATTCTTTTAAAAAATAAAATCTTTTAATCCCAATATTGTT
TTAATATTCGAGACAACATTGGCATTAGTTGGAGGTGGAGTAACAAAATTCTGCATGTCA
AGTTCGAAACTGGTGTACAACAGTATTGAGCATGTCAGCTTAGTCACAGTGATGTTACAG
GTTTAGTCAACTGTAGAACTCCCTGAGATGGCTACTCAAAGTTCCAGCATCCATGGCTGA
GTAAGAACTTGTTGTTCCGAGAAGAGATTGTCCAAATGTTCCATGTGAGTTACTGAGTTC
CGTAATCCTATTATAAAGGCCCTAACACAAGCCGGGATTGAATTGGAACTTGCTGGCTCA
AATGGAATAGTGGTGACAAAATCGAGAAGTGAGTGGCAT
<110> REPUBLIC OF KOREA(MANAGEMENT : RURAL DEVELOPMENT ADMINISTRATION)
<120> Novel bacteriophage specific for fire blight and use thereof
<130> PD21-342
<160> 1
<170> KoPatentIn 3.0
<210> 1
<211> 53559
<212> DNA
<213> Artificial Sequence
<220>
<223> Erwinia phage Fifi044
<400> 1
tgttccatgt gagttactga gttccgtaat cctattataa aggccctaac acaagccggg 60
attgaattgg aacttgctgg ctcaaatgga atagtggtga caaaatcgag aagtgagtgg 120
cattcgggtt cagtggcgtt tcgttgcgtt aactaagccc gaagccgaca taaaattagt 180
aacaaaattt gtggcagtat ggcatgttaa tatcgtttca atattgcgat tacttctgca 240
tgttaatatg tgaccgattg ttagaggact aatcacctcg aagatacgat tgactggtca 300
aattggcatg tgactcgttt gcaatattgt gacaaataga tgcatgtaat attgccacag 360
ttttctgcat gtaatactgt aacccgcatg gttactgggt ttggctaaac ctgttagcat 420
aatcacaggt actatatttg tgacaatatt gggaattaaa agatttattc tttttctttc 480
ttttgttggc tggtcactca actgtaccag atttctgaac tttgaatgtg ccaaatacat 540
ttgagtttcg agtctgggat agttacaaga cccgcatgtg taatgtgaca attaagtgag 600
atttggcctc acagtattgt tccacattag ttgcatgtga atgaaagaca aagagtttat 660
ctctcaattt attcggtgat tttggctgag taagttgcga gataggctcc aaactatcat 720
taggatgtac atcgggcact atgctagtca catggtgccc ttcttaattt actttggaga 780
tacaaaatta tggctcgttt cggtgatttg aaagatggtc aacagtttgc cgttggtgaa 840
actgtgttct ggaaagtttt ggaagaatct gaattctata atgctgtcga atgcaaagat 900
agcagcaaag caatcatgat tcttgacgat tgtgaagtag aattggagca cgaataatgg 960
caattctgtt tatcaaatat cgtgaccgtg ccaacgaagt tcaaatcgaa ggtacgttgt 1020
ctgaattgaa agttcatctg aaaggtttgg atgaaactgt tgttgaatat gtactgttgg 1080
aagatgttga tggtacaact ggtgatgata ttgaatcact gattacatac tgtgaatttt 1140
gggagacgat tgaatgagtc gtttcgattg taagaacaaa ttgtatcgaa ctcccggagg 1200
acgtttgatt ctccggcatt ggattgagca acgtcacggt tgggatttct atatgagaga 1260
ttatgatgat aaccggtggt atcgtgctgg taatcaactt tactgtgaag atgtgtgcaa 1320
ctatcaagcc aagctcattg gcatcaactt gtcaaacaca ggtctataat gaaaactggt 1380
gaacgtgttg tgatgaagca tgttgaagct cgtctcgacg ggttcactgc ttacaaaagt 1440
tatgaagttg tctcaggtga aggtgaagtt aatgtgagtc caattgcttt aaagttgggc 1500
acaatgattc accacagtga gagaactttc aatctaattg atgataaagg taggacacgt 1560
ttttgttcta cagctcattt tcgtccgttt aatgcagaat tgggaggatt attccagtga 1620
ttcaattaca aggtcaaccg ctccaagatt tcctcgcatt tctgtccgac gcatatcctg 1680
aaataaatgg tgccgaattc gtaaataatg gcatcatgaa tatctcaact gattcaatgt 1740
tgtacaaagc atgggaagaa ttcgatgaat actgtggatg atgaaagcgc agcctttcac 1800
aactctggct tacctgtaga ggtataaaaa tgtcaagtct gattgcgttt aataacgtag 1860
ttcgtcgtgc tcaaactaaa gccgacaatg cagtaagttt gattgagaat gttccaatca 1920
caactaatgc agcaattatt ctgtttgcgg ataaacacat tcgcatgatg gaagagcaac 1980
aagcagcaat gcagcgtgaa ttaacagaac ttcgtgcatt ggtggctgta tcaagataat 2040
tttcataaac tggatgtggg ttatggcgat attgtttctg agccagaaca aatcgcaact 2100
cgtcaggcat caaaccgtta aagtcctgac tccagttaca ccaagcgaat cccggctttg 2160
ggcttatgcc ctgtttcacc attcgcgaag gtttacatat caagtttgag ttgacaacaa 2220
tgaaactccc gatggctcaa taatgcggtt cgagcgcaaa aaggctggat actccagctc 2280
ataaacgtaa gtagcttttg tattcgttct cactacttca tagcaggatt aagttgccgg 2340
tgactataag cagtctgaac aaataccaaa tgttatttaa atgtcggcaa gatgcagcca 2400
aactatcatt aacttcatag gagcagtaaa tggcgattct acgcagaaac aaactcggtc 2460
aaatgaccaa agcccgcgtt cgtgttggcg acattgcaac gatttcaaaa tcttcaactg 2520
ttctcggatt aaccgcgaat aaaggatata aagtctatgc aacgtcacta tcgagcgagg 2580
acaagcgttc ttatattggc gctcattatc agtgggcttg tgttactgaa aatcactttg 2640
tcgtaatcaa cgaccgtggt gagaaaacct tctgttcttt gactccaaaa catccaaact 2700
atgatgttgc agcgagcaag gcaaactttg gcgaatggac ggtgtaatat gtcatattac 2760
gttgatgaat tgggtgtagt tcattttgtg aatcctcgca aatgaagtac gcaaatgctg 2820
ttaagaacga ggacggaagt ttaaagaatt tcgtcctgac agttgctgga aaagacttta 2880
actgttcttg tggttgcaat tgcttccaca agccagaaaa agagaatcca aaaatctatc 2940
gctgcaatgc ttgcggcaca acttatggaa gtcgagaacc gaaatgaacg aatttctgaa 3000
ttattctatc ctcggcatta tgacggtaca aacgtatctg attattgctg caattgattt 3060
attcttgcaa tgctggttga gcaagaaaca tcgtcatttc agtaatcgtg ttgcacgagc 3120
gctggcatgg ccaattttga ttgttgtact catcatcatt ttcgttctgg catcatttgt 3180
tggcctgtat gaaatcatgt cgaaggcgaa gcgcaaatga acaaaggtga tttagttgaa 3240
tgctgtgaat caatcggcaa agctttcatc aacaaagcca gcatcaaaga aaacgcgttc 3300
actaaagggc gtgtttatga agttcttgct ggttacggtg acggtgatat cagtcgcagt 3360
gatggtcgaa ttggtgcgtt tatcattaac gattaccaaa tgtgtgttaa agacgacttt 3420
ggcgatatcc gcttaattaa gtgtgaccat cgtttcaatg ttgtgcattt gagtccaact 3480
gcgaataagc gccgtgttcc ggtgactcat ttgtatcatc caattccagt gggccgatag 3540
gccaggagca actttatgtg gtatggttct tcattcaaaa atggttatgg tggcgaatca 3600
tttactttcc gtaaagctcg caaatcccgt aacggtggct tgggctggtg gattacaatt 3660
gctgtaatct tctggctctt ctttggttca catattccga caattttctg atatgagaac 3720
atatataaac agatggaagc aagatagaaa gaggcatctc atgtctatgt tgtctgaata 3780
tcaaagtgtg aaacaatctt tgatacacgg actgcatggt cgtgacagat atctggagaa 3840
atctagattt aatgtaaatg attactcttt ctatgctcca ctcattcgtc aagtacaaaa 3900
gaaaattaat ctgtatacaa agcgcattca ttgagtgcgt tttaatattc acgattagtt 3960
ggatgtgaag aacagcaatg acgctgtaac ttaaactgta aattacttaa ataaagaggc 4020
tttatcatgt ctaataccaa aacttacgac gatacaaaca ctggcatcat cggcgcaaac 4080
gactacaaac agaaggatac tcatcctgac caacgtggtc gttgtaatgt aggtggcctg 4140
tggtattggg tttcaggttg ggataagaaa tccaacaacc gcactttcac atctttggca 4200
ttcactctga tgactcagga tgaagttgac aaaatgatgg cgaaacgtgc agagaaagaa 4260
cgtcctcagc agggtgctca acagcagcaa ccacagcaac aggctccggc tcaatctcag 4320
ccacagcagc aagctccagc tcagcaaact ccaccagcga ctcagaacaa cgaaccgcca 4380
atggattttg atgacgacat tcccttttga tttgcagcga gttcttaaac aagagcgcag 4440
gatgctgcga gaaatcatag gtaactaatg agccaattcc tcgaaactgt gtacgccagt 4500
gaattacgtg ctggcgacat tatttattac gagggtgatt tgatgcaggt agaaacaaac 4560
tacctgcatc gcaattcacc atatgcgaaa cttaatttag ttccggcaga gaatccaaaa 4620
catattccac tgtctgttaa attaggtttg ttcacgaaca ttcagagaag aacttacgtg 4680
cacacaaaac atctgcatat tagcaattca attgagatta tgtctgagtt gaacgtgctg 4740
cttgagcagt atgacaatgc gaacgaatta cctgaaaaag tgatgttggc aaacaccaat 4800
ttcgaagttg attcggtgat tgttcagcaa atggtgcacg agaaaatgcg tgcttgtttc 4860
aatcgtttga aaaacgaaat tgaaatgatt aagaaaccaa gttaattttg agaggttgtt 4920
ccgataggag tgacctcaat taagttaatt tattcaacaa agcaataagg taacaaatgg 4980
ataaggaaga ggttcaaacc tttgacgcaa catacgatac tctggttcaa attcagacaa 5040
agcaatcaaa gatttcttca ttgattgttg aattcgacca agaatatcac ggtgaagcag 5100
acccagccaa acttaactct atcaaggtta agatggcaac actcagcaaa gaaattggtg 5160
aacacatcaa ttctctgtga gttgcagtta aatcttacag gttcttatct ccgataggag 5220
cctgaatagg ttcaattgga ggtcctatgt acgtctacta ccaaaatttc atttattgta 5280
gtgattggcc taaccattac aatcggtcca tgtacacgca tcaaattact gttcacgaga 5340
atcaatctcc tgagcagtat gaagcattgc gaattaagtt ccaccagcat aaagtttcgt 5400
acagtaagtg gacaaagaaa gtatacattc gtgtcttagc cagacgtgtg aaggtgaata 5460
aaactcacaa atctcatcct ttctatggaa agatgacaac aacccattgg gcagaagttc 5520
gtaactatct gctaaacatt aattacagga ttgattacga tgaagatggt actaaaatta 5580
tcagcaatgt tctggattag tttcgcgatt ttagttgttt atggttgttc gcatccagtc 5640
catgcatcaa tgcatcaagt cacctttgag tgcaatggtg agcgtctcat ctttacccaa 5700
caagacaaga atcccatctt gttggaggtt gttcggacaa aaggaatctg gatgcaggtt 5760
ccttcggaca agccagtcca aatcttttac aatctcgact ctggtaaagt tgccaaagtc 5820
gggaagaatt attcaggtgc atactatgtc gaattttata aagacacaga ttatgcaaac 5880
gcatttaaac cagagagaac attgccgtgc cggacaaaat agacctcgaa taccaatatc 5940
gccgtctaac tgccatcatt gataaagtca agaatggaca tttggtgaaa ggcaaagttg 6000
ttgccgatgc tggagaagga ttagcagttg atttggattt aacatccgat gagctgttgg 6060
caattctcca ccagcgtgca ggcaaagttt tcaatcagtg gagaaacgct aaagttgaaa 6120
ggtattcaca gccagttcaa aattgacgga accgactatc ttatcgagtc cgagtgctct 6180
caagcagtgt tcccacgact taccgataag gtaatccgtc atttggagcg catcggtaaa 6240
catgccgctg cggaaatgta tggccatgaa gctgttgttt caagctatac gaaaatcaat 6300
aacgagttag tgaaattcaa actaaccgta aatccaaaag gattaccatg cagttaaacg 6360
aattaggcgc agctgttcag gctgccgctg atgccgctgc tgaccagaaa tcttttgtcg 6420
ataaactgac tgagatgtat gactcaggca caaaaactct tagtgtcttt gttgaagaaa 6480
ctcagtcacg gacagaagtt gacctcgcat ctttctggcc gttgcaaaat gacaaactgg 6540
tcaaattgat gcaataccaa ggcaatctat ccggtattca aacaaatttg cagcaagcgc 6600
tggaccaggc aattgatgga ttaacaaatc caccagctgc aaacgcaaca ccggttggag 6660
aagtttcggc atgagtaacg tcaaactgag tccatcaagt caggcaaagc tgaaccaagt 6720
ccgtatggat ttgcgtcagg tttgtgcgaa ggcatttgaa accttgcctt ttgatgtgac 6780
tgtgattgaa ggtttacgca gtcagaaacg tcaggctgaa ttgtttgcac agggtgcaac 6840
caaagttatg acgtctcggc atatgtccgg caatgctgtt gacttggctc catatccaat 6900
cgactggaat gataccgaac gtttccgcat cctcgcgcat cacatgtttg cagcggcgaa 6960
ggagcttggc atcaccattc gttggggcgg taattggtcg cggattgatg aaaatcaaaa 7020
gccaccatca agctttgttg acatgcctca tttcgagttg ccagcatgag tactcaaacg 7080
gatacagaag ttttccttct gaccgaagag tataaaaggt tggaagaaga gcgtgccgaa 7140
caccaaacca actttgattt gattgttgaa tcgttcaaag aaccggttcc cggtggtgtt 7200
gttacggatg actcagttgc agaaataatt ctgatgaacc caggccgtgt caaacagttc 7260
aaagtttaca ctcagtttct tcttgcaggt ttaaaacacc gcatggatga aattgatgca 7320
cgcaaagtga agctggatga gttaatcatt tatgcaaacg atgcagtaga ctacccacca 7380
gcataatcca tagcggagaa tccaatggac tataaagatt tgccagaccg catcaaagag 7440
ttgaagcgtc acaacgaaga ccgcaatcgt gcagtgcgtg agtgcgatag accaaccagt 7500
aaagaatgga tggttgtcca aattgaaggc gactatgttc gtgttagtgc ggaacattta 7560
aaggcagcaa tcgccaaaac aaccgaagaa gttgatgttg agttatccaa actcacagac 7620
gttcacgata cgttgaaaaa ggttgccagc ggcttgctgt aataggccag cagtgaaaga 7680
aataatgcaa cggcgtcctt acatggacgt cttaaattcg gagcaagtta tggacgggta 7740
tagttacatt cgaagaattg aatcgacaaa ccatgttcgt gatatttgtg ttcgatttta 7800
tggtttgaag tcaggaactg atatttggtt agatgcacaa gcattgtatc aagccaatcc 7860
attgggttct gtaagtatgg caaccttcct cagaactcat tgtgagattg aagaagatgt 7920
tgctgaacga attgcaaatg cagattggga agttgttgca gcagaaggtt tgtgcgaacg 7980
ttgcctgact cagccgaaag gctttgattg ggaaatgtac gaagaaatca gtgacgctcg 8040
tagcgattat gccgacgaaa ttgttttagc ggcgatagcc tgcgatgttc cattatccct 8100
tattcaagac cgctacattg gcgagttcaa ggattgggaa tcatttgtca aagaacgttg 8160
ggacgaactc tacaaagacg aagttccaga attcctggag aattacattg actatgacaa 8220
aatcacagtt gattggaaag caggtggaga atactccgaa gacaacaatc atttcttcgc 8280
aacaagctga gccaacaaag tggactggtg gtgaaatcat ttatcaccaa gaccgtcagc 8340
actattacca agtcatcaac cgttgttcag gcaaaggccc gaatggatgg gttgaaggct 8400
ggacatatgt tcgcgtgaag gatttgtttg gcaatcaaac agcgtttgaa gttgattgtg 8460
acccggacaa gttcaagatg tacttccgac cagacgaata ttttgattct gactggattt 8520
taattcgtgc ggtaattttg tagcagtgtg agcgcttcgc gctttcggca ttagctggag 8580
gtgcgaagca cattgtaatc acttggaaca ttggtgttcc agcttggcac gttggtgtgt 8640
tcccacaaac aactaaagtg tggaacgtgt gctctgtagg tcaaggacat tatacaaaat 8700
tgtgtcaagg gttaataaac acattgtgac gatagatgca gccacatcta ccggaaaaag 8760
tggttatgac caaaaccgat aataacggcg tgaaactatc gttaaaacag gttagtcaca 8820
aagtctgttt tgagatagcg ttatgctatc attcacttgc actattaact tttaacaata 8880
aaccgtgaga atacgaaatt attcccgcta tgttgggttg gtgcggcgaa gttgtaacta 8940
caatataccg gcgagacata tttcaacctg ccttgatgat tgtgagccat cataattatc 9000
tttaagggca cataatgaaa ctacatgtgg gtaaactcga ccgggtgaat gtgactattt 9060
gtaccaagct gttagggctg ataattttgg gtaaaaccag tggtatagat agcgattctg 9120
cgacggcagc ggccttgtgc cgcgctttag cattagttgg atgtgattac atagggtagg 9180
gcaagaggtc agccacgtgc gacacaggga gccatacggc attgtcggtt tgaggctaca 9240
cgccattgtg cggccctttg ccttacacct atgcaatcat attgccacca gctttcaaac 9300
gcccagcgcc acggagaatc ccgtgctgag tgggtttcgg agcggttcct tgccagccaa 9360
tgaggaacca tcagtcacat ttgcgcgttt aattggagag aattcctatg agcaaatgtt 9420
atttctgtac acgtcagttt ggtttgctgg aggtttatta tccagttcgt aaacaagctg 9480
taaagaatgg tttgtgggtt caggttggga agtgctgcga tgattgctgg caaccaacat 9540
tgaaactcaa tatcaaaact tgggagaatc aatgagcaac gtaaaaggtt ttatcaccaa 9600
cgctgacctc acaatgcatg tggaatccca gcgatatatc tctgaatgga tgactggtgc 9660
gccgttggaa atgatgttgg cagcaacaag tcggagactt gttattactt tggaagctaa 9720
tattcaggaa attaagaatg ttagcagcaa cccaatgaat gaaattcttg aggacttgcg 9780
tcagcgactc agtgaacatc ttggcggtaa acctgttcca aacaatgcaa ccaaagaatt 9840
gttgcagaaa attgagaaca tggccaaaga cattgagagg gtttcgaaag aacgtgatga 9900
ccttcaagct gttgtggatg ctgcggcaga aagccaacgc cttgccatgt tccattcaag 9960
ccgtgccaaa gaacatcgcg caaaccttaa agaaagaaaa ggtcgttaac atgccgcgta 10020
aaattcgaaa gcattctgca aaagttacat ctgtcgcaat gaaagatggt gatgtatatc 10080
actatgtttt agttggcggt aaatggtttt acaacggtag taagaacgcc aatatttcat 10140
cgggtgtagc caaagatggt ttctggtttg acccaattgc tgaaataatc acacgctcgt 10200
gtggcgggta tacaaccaaa actttgaacg atgtaattac ttacatgggc gaagaagtta 10260
gtcgcaaagt ttatatgcgt gagtgggaat ccaacacgta ttatggaaat gaggcaccat 10320
tttgatttac ggtaaattgg ttctaacatc cggtaaggtg ctgcacgaag gattaaacat 10380
ccaagaagtg ttgcagcttt cacatgacat ggacgaatgc gaacaagacc tatcagaaat 10440
gatgggtggt gatttagcac tcggtcctca acttgtacgt gatttggcaa accgaatcat 10500
tgaactggag agcaaacttg gctcgcaaaa ctcttaaaca aaagctgcgc gcgaagttgc 10560
gtgtagtgaa aaatctcaac aaaattgagc acgaacttgc tggcatcacc atcaacaatg 10620
aatgccagaa aactcaggct atcgcaatgg cgcatggttt gatggcaaca ctgttgtctg 10680
tgaaacacag catgggtgtt gacgcaaaag gttttgatga agttgaaaaa cttgcacaag 10740
gccgtattgc tcaatgtatg gtgcagttgg tttcaatggg catgagcgca gaagaagcta 10800
ataacagaat catggcaggt cactgatggc taaacgcaaa cagcgaactt tcacggaagc 10860
cgatgttaac tttgctgttg cattagctgt aagtgattgt gtcaatcaaa ctcttgagtt 10920
gctgctttta agcggtgctg aaccttccgg cgaaattggt agaatcatta tgcactttgc 10980
aaatggtaag acccaaacat acgccgaagt tcagaaagcg ctcaaagaaa ttgaccacac 11040
aaatcctgca aacatggaaa agattcaagt cttgcaggag attgtaagtg gcaaagcaaa 11100
atgagcaaga ccgcatcatt gcccttgaaa cggtgaagat gtggttgcag gaacatgacg 11160
ccgtgtatct tgcaaaagaa aacgtagtaa catattggga gcacttctca cctgattcca 11220
aaaaaggtga gtgggttaaa ttaaaaccat atgaatgttg ccgcgtcatt aaagccacac 11280
gtgctggctt tgatgcaatg aagttgataa agcctgaact actgatgctg gctgcacaag 11340
aaagtgaacg tgcatttaag caagcagtat tttctcgctc caatactcca agtgagtatt 11400
tcaatctgtg tcgcactcag catttcaacc ggctcgaact tctaactctc tgcatgttgc 11460
aggaattagt tggacgtggg tggaacgttg aagcagtgtg tctcggcaaa ctcatggttg 11520
aagtcttcta caaacatggt tttgtcgttc caaaccgaac actccgctgg cgactgctgc 11580
gtgcagttgc agatgaagct ggtgtcaaaa tccgcgacag acttgaccgc ttaaccgttt 11640
atggtgtagg tcgatttgtc tgtgtgcaga ttgagggcat caacgattca atcaaaaccg 11700
aatttaccga agacgaaatg gattctttat taaaagaaat cgttctgcgc ttcgcgcaaa 11760
tttgattttg tttttgttgt aatattggag caattcttcg gcattagctg aatgtggaaa 11820
tgctccattt caaactttta ttccctgatg cgaacaccaa cacaaaatct caggtgtcca 11880
tctatccaac ataaggattg ccaaaatggc taaagtagta acggttactc agtacgaagc 11940
aaacgacggt tctctgttcc tgagcgaagc agaagcgaat gcacatgatt tcaaactgga 12000
aaacggcgcg cagattgaag cggctgctga agcgtttgta aacaccattg gtgcaatcga 12060
ccgttcgcgc aacatgcagg tcaacactgt gaccaacttc ctggcattct acctgccgtg 12120
ggttgaagca ggtcgtccga ccgttgagcg cactgttttc gatactccga aagaagcgaa 12180
agtggttgaa gatgacgcgg ctaaagctgc tgatgcagaa gttgctcagg ctccagttga 12240
agccggcgaa gaagctgcac cactgttcta aaatcgatct agctggcgtc tggtaaacgc 12300
agcgcaaatc ccgatatgat tgtcgggatt tgaaataccc ggaatcccat tctgccaacc 12360
acagcacaga ggctttgagc cgatgccgag agttgagagt gggcttctgg atgatttgaa 12420
taatccaatt tgtaagatga ccgtgcacac aggacttgaa atagtaactg acttgagacc 12480
tcagtgaatc tctcagtatc cgtgaggaag tgcacttgca gttggctgaa agacggatag 12540
gaacctcaac tcctaccgca ctcttagagt ggtcatccag aagttttcgt tgatgagcga 12600
ttgtttttat tcatttaaaa taaatgcaaa cgattctgaa atgcgagttg cagcctaatt 12660
aaatttagtc tcgctccgtg gtcttccgat tccatgtaac cgaattcggc gcagtggtac 12720
gctgtgatta ataatgtatg ttttctgtcg cctccattct tcggagaatt ggcgctttac 12780
ggttcgagta ttcgtagagt ggaggtgaac gaaagcactt tgagttgcat attggattag 12840
atgcgggagg atggccgatg cccgttatgt catggcccag caatgtgcaa catcaaagtg 12900
aattaagaga gctttagtgg tatctgaatt aacagacgcg gattagtctc aatgggccta 12960
agacggtttg cgaggaacac aaactatttg aagtcgctct tcaaagaacg tgtggtatgc 13020
gcactcagat ggaggttcga atcctctgtc cgcgagcact aaagaagaac cgaaaggtta 13080
caagtaacac gaggcgacta tgcgcgccta ctcttaataa agaattgaag catacatctt 13140
cggtaagcct cttgtgcaca aagagtgtaa ggcaggttcg actcctcgca tgtatgttga 13200
aatattgaag atagacgtag ttgtgggacg tatactctac tactacgaga caacacattc 13260
acccgcgtgt gtcttctctt atagaagtga cggctaagac ccggagcaag cggctttaaa 13320
tttaaatcat ggagaacacg atggacgaaa gaatgtcact cgcgatggag tattatgatt 13380
tcatcatgac ttgcgaaatt gctgttgcct tggcacgcaa agacaaacgt cctgagaaca 13440
aaactcttcg tgaatgtatt aaagttttat tgaaacgctc gcagcataaa gaagtatctt 13500
tcatcttcaa aggtgtgaag aaagcaaatt atcctgttgg cgcactggcg aaaattcgtc 13560
gcgaattcga tgaggcattg ggatgactaa tgaagaactg agaattcaag cagaagcaca 13620
cggattttct ctgcaagagt ttgaattaag aattcaagca gaagtgctca ggcttttcac 13680
aacaactgga aaagtcccgc actttgatga agcgcatcca atgtttaaag cggcagttgc 13740
aaaatacgcc caggagttaa aggccaagat ggacgaattt gaattaaacg aaatcgactt 13800
cgatgaagtt gaacgacgca acgatgagaa attagcgaat caggaggaaa tcctcgatga 13860
atctgattgc gatggttgca aaatttaagg gatggattgc gactgcattc gtctttttag 13920
ttggtttggg tgttgcctat ctttctggta aacagaaggc agagcaaaca gccgaagtcg 13980
aaaagaagga tgcagaaatt gaatccacaa aagctgttgc cgacacacaa gtccgtgaag 14040
ccacggctgc aaagaaagtt gttgagtctg ttaatcgctc tagcgatacc agtgttgacg 14100
acgagttgca gcgtttcaca cgagactaga gttgttgata caagctgttc cagttttagt 14160
ttgattatgt tgtcgaagaa ggaaattcct ttattgactc cagaaaccaa acgacaaatt 14220
ctttctcata atttaattta tgaaaagaaa tgtgtgccgc caaagagtgg tacacagcct 14280
gagtgaaatt gtgtggccta tcaagccgag gaaacgaacc ggagtcttca ctgttgttct 14340
ctctggacac aattgaacga aagcaagcct cgcagttctc gctggaaacc tgcgttatca 14400
aaaccggcaa caaataatgg tggcagagac tggtgtctcg ggttcgattc ccaatgccgc 14460
ccccttattt gctccgtgca ttgtcactgt cagtcacgat ggttctgtct caccaaagta 14520
gatggagtga atgaattcga acttacccga caagttgttc gataccagcc ctgagtatgg 14580
aaaataactg ctcaacatag tcgagggagt gcgcgacttt aaacaacgag actcaaaaca 14640
aaatgaagct gccagacttc aggtggtcac ttcctaaaac agtgtctgag aacacactca 14700
ccaaagttga atcccggtta ttgggctccg gtaaataaac aaaagcctgc caaattacgg 14760
aagacgtggc gaaattggta aacgcaagct gaaatgctac cgctgttggt tcaagtccaa 14820
ccgtctccac catatccttc gatacagttt tacgtctgtt caaaacgtaa catgcgctct 14880
ggcatactgg agtcaaatcc agacggacag tttctgtaac acaattcaga aacgcggtgg 14940
tgagatttgt tgcggttgca tcgtaccaga ctgtctagca gtctaacgag tcaggctcct 15000
cactggcgat tgggttaacc tccgagacgc aacattgaaa tatgcaaggc agggtttcat 15060
atgtcatggg tttcttcatt ctcccatgaa aaatatgaca ggtgttacga gcctgtgatg 15120
ttctcccata tcaagaagtg attccgcttg gccggttagg aatcaaagtc tttttgttct 15180
acactctgca cagttaaagt gtacgatagc ttattttcat tcccgtgaac gtagagtgta 15240
gaacaaagag ccataagctg agtaatcgtt ttaataccca tttgagagag aattcatgag 15300
caacaatgat gcagttctgt cgcagcttat cgctgaggca gatgaagcaa atctagaata 15360
tcttcgtgat gatattgcac ttcgcgcgat ggaaagaatg ttaaatccag aaactggtgc 15420
tgagatttat catcaaccag ttcatgcaac aaaactcgcg gtaacagctt acgcaattgc 15480
agatgcaatg ctggcggctc gttacattga gccaatcaaa gttgaaccgg aagttcatta 15540
atgtccaaac aaatttcagc tgtactcgtc gataagatgg gcacagattt aacaacagtc 15600
aatgctgcac gtgtctcata tggtgcggag tctcacgaat ttagtgagcg tgatgaacat 15660
cttgtcaact ttcttggcga gcataatcat gtaactccat tccgtcatgc tcatgtaact 15720
ttgcgctgcc gagctccaat cttccttgcc cgtcaattgg gtaaacatca ggttgggttc 15780
tcatggaacg aaatctcgcg ccgctataaa gatggtgtag cgattgagat tgaaacttac 15840
gaaccagaac ttgttcttag tcgcccgcat aagctgatga ctcaatctgc acaacccatg 15900
cgtgaagact acgccagtga gatgaaactt ctcattgctg cggtgcaacg tgcggctgtt 15960
cgtgaatacc aaggtttaat tgatttgggt gttgctccag agcaagctcg aatggttctt 16020
ccacaaggta tgattacaac ttgggtgtgg acaggaagcc tctatggttg ggctaacttg 16080
tacaagcagc gttcatcaga acatgcacag tacgaggccc gtctgtttgc agaagaagtt 16140
aatgtaatca tgaaagaaca ttttcctaga acttggaaag ctttaacagg acaaggttaa 16200
tgcataagac attgccagca agattgtatc ctgcggcagt atctgcaagg ctgtatagtc 16260
catatttggc ctctggatat cgctactggt atctaaacat ctgcggcact acatactgtg 16320
tggcagaaca caggttgaac tactttatca agtctaggaa ggttgttgag aaagttgacc 16380
atgtggataa caacaaactc aataatgctt tggataattt gcgtgagtgc acaacttcgc 16440
agaataacca caatgcaaaa ctaaatgcac gaaacacttc cggacataaa ggagtaagtt 16500
tcaacaagag gctaggcaaa tgggcagtta atgtctgttt gaatggtaaa ctacatagcg 16560
gcggtgttca cgaacatctt gaagatgcgg tgattgctgc aacaactctg cgaaagaaac 16620
tccacggaga gttcgccaga gactattaag gactccaacg agatgcaagt caaacagtgt 16680
gtagccaaaa ttaagcacga tgttcccacg tgtaaatcaa caagcgggaa atccctgcaa 16740
gtttggttga acgaaaatga ggatggaact cgcaattatt ctggttattg ctttgcctgt 16800
tctgttctcg ttcccaaccc ttatggtgac aatccaccgg acccaaaaga aattcacgtc 16860
aaaactccag aagaaattca ggaagaactt gatgagataa cttcttgtcc ggtgtttaat 16920
ctcgaccatc gcggcattga accagaatac tggagccatg ctcgtgttcg acttctgtat 16980
tcggaattcg atggtacaac tccaaatgct ttagcccacg gatatactcg tggcggcaaa 17040
cttgttcgtt ggaagattaa acttctgaac aagaaagtga tgtggagtgt tggtgatact 17100
caggagaatg acccatacaa ttgggaagct gccaaattca ttggtggtaa aactctgtat 17160
atcacagaag gtgaagaaga tgcgattgca ttgcgtcaga ttctccgcat cttgaaccgt 17220
ggtacagcat atgaggaact tgatttcgca atcatttcct tatcagatgg ttcagattca 17280
gttcataaat gtctggctcg cgtagccgaa gagattaagc aacgctggga aagcgtggta 17340
atcgtctttg acaatgatga accgggcaag aaagcagcga aggaagcatg ccgtttatta 17400
ccgggtgcaa tggttgcaca actcccggca aacgatgcca atgattgcct taaacgagga 17460
atgttaagac aaactcgtga tgcggtaatc ttccgtgctg cacgtccgtt gccaacagcg 17520
ttggtgaata aagaattatt aatgttggaa cttgacgatg aagttcctcc gggtgttgat 17580
actccgtggt ctaaattgac agacatgatg ttcggtcaac gtcgcggcga aattatcaca 17640
cttggtggtt cagaaggtgg tggtaaaaca acactagctc gtcaactctc agaacacaac 17700
atcgttaaac atgattgggg tgtgttcact tgtttcatgg aggaaactcc acaagagaca 17760
ttgcttcgaa tggctggttt gcatgatgga ctcccatatt gggaaccttc attcagacaa 17820
gacccacgat tcgatgaaga gaagttccgc gcaacttgtc gtaaaatgct tgcgaacatg 17880
gagatttggg atagaaagca acaaggtgaa gacccgtatg aaacatggga aggcatcaaa 17940
actatcctgc gtcaaattgg tccagacatt gacatgtttg tcttggataa cttaacaatt 18000
ttgtcagaag gtatttcagc gtctgagaag aatgatttcc tcggtaagct ttatgccgac 18060
caagttaagt taagcgaaca atatcagttt ggtatcctcg acctatctca ccttaacccg 18120
gttccgaaag gtcagcgtcc tcatgaagat ggtggacgta ttaagaaggg tgacttcact 18180
ggttctcgtg cagcatcaaa atattcccat ttcatgattg gtttcgaaag aaacagtcag 18240
gcgattgacc caaactgttc aatcattcgc ggtattaaag cccgtaagag tggtaagaca 18300
gatggtttca aaacatatta cgaatcagat tctggtcgta ttattcaacg caattgggaa 18360
gatagtttgt ttgaaacgaa agaaatcgtt cagctcacta agaaaacaac cccgcaccaa 18420
taactaaggt cttgtcctcc tactgttgtg aaacagaagc ttggacaaac ctcctcaacc 18480
atccttggag acaaaattga tttatccttg ggaaagaatg tctgccagtg acattgaaac 18540
gactggtttg ttagagcaaa tggcgaaaca ggaacatcca cgtttgcaca acatgggtgc 18600
gattgatgca ttaacgaaag ttgaaaattg tatggagtgg actgaacgta gtagaatcca 18660
ggcatttctc gacacggctc caactctcat catgcacaac ggtttgtgtt ttgataagga 18720
ggcattagcc ttccttggat atgatgtatc caaagtaacg attattgaca ctatgttcat 18780
tagctggtac ctgcaacctc gtcgtatgag gcatggtttg gcagaatacg gtgaagaatt 18840
tggtgtacca aaacctgtaa ttgaagattg ggaaaaccaa actcaggaag aatataactt 18900
ccgtgtaatg caggactgcc gtattcaaat ggcattgtgg gaacagcagt attcccaact 18960
cttagcgatt tatcgcaccc cggaagaagt gaaacgcttt gtcgaatacc tgatgagcaa 19020
aggccgtcag caagtcattc aacaacgtac ccggtggaag ctgaatgtac cgaaagcaga 19080
agcttttcgc gacatgcttg cgccaatgat ttcggagaaa gaaattgcac tcgaagcgtc 19140
gatgccaaag gttcctaagt atgcacttaa gaatcgtcca gccaagtgtc ataagcaaaa 19200
tggaacattg tctgcaacag gcgccaaatg gaaagcagtc tgtgatgcaa atggtcttga 19260
ttggcaggac aaaaacctcg tcataaaagt tttaaaaggt tacgacgaac caaatgctgc 19320
gtctcatgct caagtaaaag attggttatt cagtcttggc tggaagccgc aaacattcaa 19380
gtttgttcgt gacaaggaaa caaatgaaac tcgtcagatt ccacaaatca cagttaagga 19440
tgatgatggt attccagata tttgtccgag cctgcatgaa ctggcggaag ataatcccgg 19500
caaaggtgtt gaacatctta ttggcctcgg tgtatatcgt aaccgcttgt ctattgtcaa 19560
cggctggctc cgtgatgtat ccgacgatgg cttccttgtt gctagttgcg ctggtcttac 19620
taacacgctg cgactgaaac atcgtggtct ggtaaatatt ccatccattc gtgtgttcgg 19680
tggtaaagaa cttcgcggca tgttggaagc accaaatgaa gattatgaac aacttggttc 19740
agacctatct tcacttgaag accgttgcaa gcaccacttc cagtggcagt atgaccctga 19800
atatgtcaag aaacagttgg caccagacta tgatgctcac ttggcaattg gtgtaatcgg 19860
tggattcatt accgaagaac aatcacaggc acacaaagat ggaatcaaga aggttcctca 19920
acgtccaatg ttcaaaacca caaactatgc ttgtcagtat ggtgcgggta ttgcgacagt 19980
gcaacgtggt gcgaagtgtg attgggctac agctgagaag ctgcatcaag cttactggcg 20040
tctgaattgg tcaatcaaag aagtagcagc gaacacaact gtgaaaacga ttgatggtca 20100
gatgtggcag ttgaatccgg taaatggttt ctggtattca ctgcgtaatg acaaagaccg 20160
tttctcaact ctttgtcaag gtactggcgc gtatgtcttt gatgtttggt gcaatgctgt 20220
aattgaaatt tgcaacgaac gttatggacg tgacccaatg ctgtctggac aattccatga 20280
tgaattgatt ctgcaagttc gcaaaggtaa tcgtgaactg tggcaacaaa tcgtgggtgt 20340
agaagcaatg gaacgcacca ataaacaatt gaagatgaac cgcgatgttg cgtgtgaagt 20400
tcaattcggc gacgactatt cagagattca ctaatggcta aactcattta cgcgttgtat 20460
gacgcacagg aaatgacgct ggttcagttt agtgcagatg ccaccatcct tgcgaaggat 20520
gctgtggcag catatctgac agagaaagtt attagcaaga cagtcgaaga agattgggct 20580
aaagatttcc tcacagcaat ttccaactca aaggatgttc cagaactttt agttgttttg 20640
gaatcctatg atttcaaact ggcaatcatg ccaatccact aatttgcaca aaactgtttc 20700
aatggagaaa caaaatggcc ctattccaag caccaaccaa agattccggt aaagcaccaa 20760
gtaaagcacc actgttagaa tcaggtggtt atccagcccg tctggtacgt ctgattgacc 20820
tcggtaaaca accgggttca ggtcagtatc cagaaccagc atacaaactg ctgggaactt 20880
tcgagctact cgatgagttc atgaaagaaa ccaacgaagc aggcgaaatc attatggtcc 20940
aagaccctga tggcgagcca ggcgaaatga ttggtaaaga cctgctggac aaaccacgtt 21000
ggtttgattt cgagtttacc tacaatccag atggatttat gggtgaccgt tctcacatct 21060
acaaattcat ggcagcggtt gacgcatttg aagttaaacc gaatccagaa gctggtattg 21120
ccggtcatcc agccaaacag ttgaatgaac tgctgggcga accactggta gttaacctga 21180
ctcagtacac caagaaaggt ggtaagaatg ctggtcagat ggcaaacaaa atctcaacct 21240
tcgcgccaat gaagtcgaaa gacaaacgtg atgcgaaagc tctggtcaat ccaaccatct 21300
tcttcgactt gggtgctcct gacctcgaag tattcggcaa gctgccgggt ggcgaccaag 21360
agtgggctgt taagaaccgc attctggcaa acttggactt caacggttct aagttgcagg 21420
aactgctggg cattgccaaa actccagatg cacctgttga gaacaaagca actccagaac 21480
aggttgatga agctctggct gctgaacttg ctgcgcaggc tgctgcgaaa gctaaactgg 21540
ctgaacagaa tggtggcgct gatgccgctg ctggtcaacc tttctaagta acacactgca 21600
tctacgttgg gtagatgtat ttaccaattg gtaaaagggc gtaaccaaga ttgttgcgcc 21660
ctcttttact ggagttttgc aaaatgagta agaagaaagt tctcctcctg accgatggag 21720
acatggtggc attcagccat tgcgcggcag aagagtacgg gaaagagtcc gaagatattt 21780
cttttgcaaa aattcagatg tctatggaat ccaaaatgga attcatggaa aagcgttgtc 21840
aggcaactga acacatttgt ctgatttctg gtgattcaaa catgcgcatg gttatcgctg 21900
atacatacaa agcaaaccgt gatggcgtat ggaaaccaga gaatttgaag aatgccaaag 21960
caactttgat tacctgttat aacggtatca agaaagatgg tctggaagct gatgacctga 22020
ttggtattct gtgtcgtaat aaagtcgaaa tgacaatggg taaacgtggt gaaatcaaaa 22080
cgattaaaac cattcgccca ttgactcttg atgactatga cgaaatttgg gttgcatccc 22140
tcgacaaaga tttgaaacaa atcggtggtc gtccgggtgg tccagttgtc aaacattatc 22200
gttgggaaac tggcacacaa ggtgagaaaa tcactgttgt ctctggtttc ggcgaactta 22260
aatgtatcgt caaagataat ggtaaaacca agaagaaaga aatcaaaggc tttggtccga 22320
agttcttcct ttggcagtgc ctcactggtg actcaaccga tggtgtaatg ggttgtggtg 22380
ttagtgaaac aaagatgtac aaaactggcg caaaggctgg tgaagaatat caaaagcgtg 22440
tcggtgtcgg tgctgtagaa gcattcgaat tgctggacaa aattgacaac tatgcagacg 22500
gtttgaaagc cgtagctaca cagtatgtta tgcgctttgg tgatggttgg caggaacaac 22560
ttctcgtgaa cggccgattg ctatatatgt caaatacggt ggcagaagga aacaaggttc 22620
gactttggca ttataatcca caggttgtgg aatatttcga ccttaattct aaaaccattt 22680
cggcgtgatt ttattcgtgt ggcattctgc ttgagcatta gtgggatgtg aatttgccat 22740
gtatggttat tgcaaaaccg aagccaggtt tacagcttgg cttcaatcag cactgagaag 22800
tgtttgggca aagcatccat caaaacttgc acttcttgag aagaagcgag tggcactgag 22860
ggttggcaac agtaagaagc caatctatca tgtgcagtgt gagcattgca aaaagctgtt 22920
caagcttaaa gaaatcgagg tcaaccacaa gaacaaatgt ggtggccttt cggatttatc 22980
caagcgacat gaattcatgg acaatttatt attggttcag gaatcagact tggaacttct 23040
ttgccatgat tgtcatggga ttgtaactta catggagcgt tacgacgtta ccaaacgaga 23100
agcaatcatt gagaagaaag taatcgcatt ctataaattg tctgataaag aacaaattaa 23160
gaaatgtgaa ctcgctggtt ttaatccagt cccaaaaacc aaaatcgggc gcaagaatgc 23220
ggttcgagaa tatcttaaga aggtactccc gcaatgacta ttaagttatt ggacgacgga 23280
acatcttata cgaaggcaca tgcccatgat tgggaagacc caaattgcca actcaaaggt 23340
cgttgcgaaa tttacatcaa gattgatggg attcgggcga ttaggaatag agacggacga 23400
gtttggagtc gcaattccaa gcctttaccc catcttgacc atttatcttt caaagatgct 23460
gaaatcttcc gcgattcatg gaatacgacc tcaagcatct tgggcagcga tgctccacca 23520
gcgtcgccgt taacgcagga aaacgtgtac gaactgtctg atggcaacat cgacaaacgt 23580
ctttacctcg gatgggctca agacccaagc aatgaaaagc ttcatgagtt gatgctgaaa 23640
atgctgaaac ttggtcatga aggcctcatc gttcgttgca aggataagaa aggaaaaacc 23700
ctttggtgga agattgtacc atataaatat gccgatgtta agattactgg cttcaaagaa 23760
ggcactaata agaacaaagg tgtatgtggc tcaatcgcaa cgaactacgg ttctgctggc 23820
tcaatggttt ttgactgtct tcaagaccac aacattgttg gcgattacaa cattcgcaaa 23880
tggttgtggg aacatcgtca cgaactgatt ggtaaaatca ttcaggttcg ttaccgcgaa 23940
gtaacggaag ctggtaaatt ccgcttccca tctttggtac ggttacgcac tgataagtca 24000
gaagagagtc tcgactgatg cagagaatca aactcggtgt ggtcttaaat ggcccaccgg 24060
gctgtggcaa agataccata gccaataaga ttgtcgagca gggcgtgttc cgtaaacatc 24120
agtttaagga tgcactatat gcgcacactg ctaagcactt tgaggttgat ttggataagt 24180
tcatccactt cgcctctgac cgtgtactta aagattcgaa atccttggcg ggattgggcg 24240
gacgaactcc tcgtgaagcg ctcatctatg tcagcgaaga aatctataag ccccgttatg 24300
gcagcgatta ctttggcaaa gttgaagtta atcgtgtgga aacactctgc gggcatatgg 24360
atggcgatgt taatgtcatc tatcccgatg gtggttttgc agacgaactt cgctgcattg 24420
acacctgctt tgacggcgtg cttgttattc gtcttcatcg agacggcttt gatttcaaag 24480
gcgattctcg ttcatacgtc gaactgccag atacaccaga gcgtaaaagc attgatgtgg 24540
atttagcaga cgaccaaatt aatattggcg ttcacattgt ggaatcagcc attaaatcat 24600
tcagagagtc tctgcgtaat gtgtcccaat aaagtccctc acgtcaattt cctgcttgct 24660
catccgctgg ctcaacttcc atcgtatgaa agtcgcggcg ctgctgctgc tgatattcgt 24720
tctgtggaag gtgcaattat ttatcccggt gatactgcca ttgttggcac tggccttaag 24780
ccagaaattc ccgaaggtta cgttgcactg atttactcac gtagtggcca cggcttcaag 24840
aacgatgttc gtctggcaaa ctgtgtcggt gttatcgact cagattatcg tggcgaaatc 24900
aaagtcaagt tgaccaacga tggcagcgct gtcttccatg ttgaaccact tgaccgtgtt 24960
gcacaaatga tgattgttcc tgctattcag gcaacattcg aagtgcagga aaatctgacc 25020
tcaactgtac gcggtgaagg tggcttcggc tcaaccggtc gcgcgtagtt gctcaatgga 25080
gaatccgagc atgtgtttac aactcaaatt tgaaagtctt ctgtgtcatt taaatgcaga 25140
agagaatgat atggctctgg aacttctgga ccaaatcaaa gaagctgatg ttcgtctcga 25200
aaagttagat gcactggaag caggtggtgt tgataactgg ggcggatacg gcgactgctt 25260
cgaggatgat gacgatgaat aatctgaacc atacctcaac aggacttgtt ggtggtgact 25320
tcatcaagtt cctgaaatac ctgtttgacg atacttttga aattgacgcg actcctggaa 25380
aacatagctc aagcaatgtt actttaggtg ttgaagttaa tttagaagtt gcaattgata 25440
tggtggatga attccaactt ccgattgctg ccgctgattt agttggtact tgggctatgg 25500
atgcctcgtc agactacagc tggggcattg aatggagtat ggttgactgc gcgtatcgag 25560
ttgagcaggt tactgaaacc aaaattgtaa cctcatggaa acccgttcaa gatgctaaac 25620
cagccacaaa agaaagtgta cctgcgcaac ccgcaagtaa aaaataatgt cggcgtaaac 25680
tttgtttacc tccgttcaac agtcacctca gccggtggcg gaacttatat cactcgtgcg 25740
ttagatgccc gtaataaaac tggcgctgat gtaattgtgt ttatgattcc gaactctgag 25800
aatgcactgc ggaacattga gcgtatcaag cgtggccgtg aagatttaat gtcagcggtg 25860
aagccgctta tcttgccttt caaaattgta gctgtaatta gcgagaatca gaaagcactc 25920
gaagaacatt tcgaagatat gctgtggaac gatattaaat actaagcgca cgccgatggt 25980
tgggaggtct ctttggcctc ctatcttctt ggagtacgct tatgcgcact atcacatgta 26040
aggaatgtgg ctatgctatt ggtgaagatg acttctcctt aatgcaatgt ccagtgtgtg 26100
attcattcat ggaattgaat cgcctgcaaa aaagaattga acctgattta gagaaggtta 26160
ttagaataga cgccactaag cgattaccag aaaggcaaac aaatggataa cgatttaggt 26220
gatgaataca acagcacggt tgtctatcat ctgttgaagt tctacggcga cttattaaaa 26280
gttagccgtg cacgcttttg tatgcagtca ctgccaatcc ttcgtaagta tatcgcggcg 26340
aatccagaca ttatcagaga ttactctgag caactggatg cagagattga aggacagggc 26400
ttaaaagata acgctgtcct gcgctctttg gttacagcac aaagagaaag gttggaagca 26460
gccgactcta aagattccac tgcgtatgcc agtgaaatcg aaaagatgat taaagacggt 26520
aagacagacc gcattgctgg ctcaacaatt gaagccgaca ctgacgaaca ctctgcaacc 26580
gacgaactca tccagcaaat tctgactgac ccactcaacg tagacccaac tgttgtaccg 26640
aaaaaggtag cagatgaagt cagaaaaata ttaacttcaa gttttgagaa gtttggcaaa 26700
tggtcatttc atatccaaat gggattccca tttcagtctc aagacttcca tgatgttatc 26760
ttcgatgtag gtcaacaaat agttgatggt aaaatcgacc gtgtgattat cacaatcccg 26820
cctcgacatt cgaagactca acttctatcc attgcattgc cgttgtattc attctgtcat 26880
aacgtgacga gccacaacat cattacttcc tatgcggaag acgttgtatt ggaatccagt 26940
ggctatatcc gaactgtaat gcttgaccca ttgttccagc gcatcttccc aaatgttcgc 27000
atcgacccaa acaaacgttc tcttgagcgt tggggtacta cgaagcaggg tgtgatgcac 27060
gctgttccaa cgggcggtaa aatgactggt aaaggtgctg gcctgttggc tgagaactat 27120
gccggttgct ttgtagtaga tgacgtaatc aaaccaaaag atgcgtactc tgatacagtt 27180
cgaatggaaa ttaatgaccg ttacgacaac acattcatgt ctcgtttagc caacgatggt 27240
gtgataacga agcctgatgg cacgttacaa aaatgtgctc gtactccaat ggtcatcatc 27300
atgcagcgtg ttcacgacag tgaccttgtt ggttacttgc ttcgtggtgg ctctgctgat 27360
aaatatcatt acctcaacat tccagcaatc gttgagaaag aaactggctc tgctgcatat 27420
tacgataaga ttattaagaa acaggcatac actcatgcga ttccaatcct ctatgatttg 27480
aaacgtactg aggaaaagtc tgctttgtgg ccttctcgta aaagtttgga atctctggaa 27540
gcaatgcaga aagcgaatcc atacacatac aacagccagt atgctggcga cccaactgca 27600
aatggtacag gtctggtcaa agaagaatgg tggaaggaat acaatcctga aacattcgac 27660
cggagccgta ttgtccgttc attcatcact gctgataccg catctactgc gaaagaatat 27720
tcagactact cagtgttgaa acattggggc gtcacgaaag aacgtgatgt atattgcttg 27780
gatattatgc ttgggaaata tgaagttcct gagttgaagc tggcaataaa cgagttctgg 27840
aagaagtgta atatctttga ttcaagatgg ccttgttgta ttccccgtgc attgtacatg 27900
gaagacaaat catctggtca attcctaaac cagcaatttg ttcgtgatgg tcaaatcagg 27960
cttatgcctg ttcctcgtga caagtctggc ggtgataagg tagcacgatt ccttaacacc 28020
attccatatt tctcaactgg caagatttat tatcctgctg gacatgaaca tcttgaacat 28080
gttaaacgtg aaatacttgg gatgacttct ttgggttcag gtactggaca cgacgattgt 28140
tgcgataacg tctcagacat ggtcgcaatt gaattctccg gcaacaacgc caactactct 28200
gcgtgggttt gaaatgagtg tgattactcg cattgatgcc cgaagcaaaa acaatactgg 28260
tttcgaaatt cgagacgcag taactggcaa aacgctggtg attgtcgaaa ctttgggcac 28320
tgaaactgaa ctccgtatta gctcaagcga ggcagtagaa atcgtgaaaa gcaacggggt 28380
taaactcaaa aggaagaaat aatgtcttcc aaaaagaatc ggcccaaggg ctcgattaaa 28440
gtcggtgacg gtcttgagaa tgtcataagt ggtatgggtg gagacaaaga taaatcggtt 28500
tataatcggt ggactttctc caacaaaaat gcagactacc gtcagctcat gaatcgcttc 28560
cgcgaagatt gggttagtca aaaagtctgc accattattc ctcaagacat gacgcgaact 28620
tggcgcaaaa ttagtacgcc agaaggacag ttggcagaca agaaattgaa actgcgtaag 28680
ctgtgccgtg aaggctacca atgggcaaga ttgtacggaa catcatttat gcttctcgac 28740
ctcaaaggta cgggcagact tgatactcca ttacgtcttg ataatttgaa gaagggatgt 28800
gtcaaatccc tgaaaattat tgaccgttcg cgactacatg cggctggtgg tattgtactt 28860
gatgcacttt cacctcatta cggtttgcca gagtattaca ctctgagcgg ataccctggg 28920
atgattcatc atagtcgttt cttacgtttc gaaggaaccg aactgccgtt gttcgaattc 28980
caaagaaaca tgtggtactc ggattcagtg ttgataccac tgatgagcac catcgaccag 29040
ttttacacca ctgcttctgc tgccgcacaa ttggcacagg aagcaaccat tgatgtggtg 29100
acggtcgcag gactgcaatc tcttctgaca aatccagaag gtgagcgttc agtaatgaaa 29160
cgattccgac tcatgaagca agccaagagt ttgtacaacg tacttattct tgacgagtcg 29220
gaagaatact ccaccaagtc tatcgctttg tcaggcgtaa aagatttaat ttgggaatac 29280
ttgaaaatcg ttgcggcagc ggtaggtatc ccagcaacac gttttctttc agcatcgcca 29340
gacggcatga atgctactgg tgaatccgac cttgtaaact atattgactt gcttactggt 29400
ttacagactg caatcttcga acctcgtctt gagattctcg acacgattgc atgtgcccac 29460
tttgggattg aagaattcac ttatgaattc tgtgatatct tccctgagtc aagcgcggag 29520
aaaacaaaac gtgcatctga tttggccatt gcgttggaga aattgattga atctggcacc 29580
atcactcgtg agaatgccaa caagattctg gaacacagcc atgtatttgg tgcgtgccca 29640
attgaccctc caccggctca acctccaact ccgccaaaag gtagtgggaa tgcaaattaa 29700
gcaaaaccaa ttcgtgcaac tgaacgacga agaaactgtt gaaggcggtg tcgttcttct 29760
gaccgacaaa gcaactttaa caactcgtcg tctgcgcgat tctggtgaaa tgattgcgga 29820
atgcactatt gctcgcactg gcattatgct ctataaagcc aaagagctgg gtgaagttgc 29880
tgcacatctg ccaccagacc aaatctgtcg tgttcgcacg aagccagaag tgttgttcga 29940
tgaagcgacc attgaaggtt gccgttcgat tccaatcact attggtcatc cgaaacaaga 30000
tgtaagcatt aagaacaata aggaattgca gaagggcttc attgaaggtc gtccggttgc 30060
tgatggttct ttcttggctg catcaattgt cctcaatgac gaatctgcga ttcgcttagt 30120
tgattcgggt gttgaccaat tatcattggg ccacaacgct ttgctggaag cctccacgga 30180
cgaaggtgtt gacttcgata aagtcaaaat cgttccgaat catgctgcaa ttgttgtgcg 30240
tggtcgtgct caaacaactc gcatcggtga ttccggtgaa gagattgaaa ttgtagacaa 30300
gacttctttc gatgcactcg aagcttctcg cgacgaactg caaaccaaag ttgatgcctt 30360
gactaccaaa ctggctgatg ctgaatctgc ccgtctgacc gatgaagaaa ttcagactcg 30420
tgttgatgcc gctgtcaaaa ctcgttttga tttgctggtt aatattgcca aactcggtga 30480
tgaatatgcg tctatggact tcactggcaa gaccgaaatg gatatgaaac gtatggttgt 30540
tgcgaaactt cgcgatgccg atatgtcaga gaaatctgac gaatacgtta atggtcgttt 30600
cgaaatggcg ctcgaagata gtgatgaagt cactctctct gatgcactct ccgtttctat 30660
caagcacggt tctcaggaac aagctgagaa gaaactaagc ccacgcgaag aagctcttgc 30720
tcgtcgccaa gctcgtttta ataaatagga ttcagtcaat gccaaagcaa gactttaaca 30780
ttaatacact gcctgctgct gctggtgacg tgtatggttt agctttcacc aactcaaccc 30840
gtctgacctt ctcgaccgaa gatgcacaag ctgcatatgg tctggcagtt caacagggtg 30900
cgaaatccaa caccatcaaa gtcggccatg ttaccggcga agtgttgggt attaccatgc 30960
gtcagaacgt gctggaatct aagacacgtc cgggcgacgg caccatcaac attccagttg 31020
gtcaaccact agctgtaatg atggaaggtt caatcaacat cgtgctgaaa actgcaatca 31080
cttctgcgga tattggcgtt agcgcaactg gtgaatttgg cggcgttgct gctggttaca 31140
ccaaagcaac taacatcaaa gccctgagct atccagccaa cgctggtgat gtgattccag 31200
ttcacatcgt tgtcgcgctc ccaaagtagc agccccggtc ttacctaaaa cggtaaccgg 31260
taaaggtgat gttacaggta agaaaggcgg ggatgtaatc gctgacacgg ctctgttcga 31320
ttattcgaat ggtgcaacta aagctgatgt aactgctgta accggcacca aagtaacttg 31380
ggataacaca gccaaaacgc tgactatcgc tgatgatgtt actgctggcg acattgctct 31440
ggccttcact cttgcgagtg gtgtgaccaa agcaggtact gttactttga aaggtgttgc 31500
ggtcaagccg taacctgaat taattgcggc caaataactt ggctgcataa ttggattgaa 31560
tagatgccaa agctgattaa actgaacgat ggttcagaat tcgaactgga tgatgcactg 31620
gttgctatcc agtctaacgc ctcagtaaca ctgagcgatg acgatgctgt gttcttccag 31680
cgtcaacttg agttcattga agctcaaact tacgacactc tgtaccctga ccttgaagct 31740
cgtgatgcgc tgggtgttga ttcaactggc ggcgctggtg ttcagactct gacctatcgc 31800
agctacaacc atgttggtaa agcgcaagta atcaatgctc gtgcaactga cctgccgaaa 31860
tcaaatatct ctggtaaaga gtattccatt ccggtgaaat cagttggtac tgcatacgat 31920
tacgacattg acgaaatcgc atctgctgcc gtaactggcc tgccgctgga agcgcgtaaa 31980
gcaatggcat ctactcgtgg ttacgagcag tacattaact ctgctgcatg gtatggcgat 32040
gatgccaatg gtttcattgg tttcttcaat aatccagatg ttaccaaagc aactgttgct 32100
gctggcgcaa acagcaaaac tggctggttc gatggcaaaa ctccaaccga aattctgaaa 32160
gacctgacca cagcggtgag cgcaatgtac agctcaactc tgaaaatcat gcgtcctgac 32220
gaagtttgga tgccggttct gcatcatcag tacatcatga acactgcacg ttctgagcaa 32280
tcagacatga ccatcttgca gttcttcatt gcgaacaacg aattcattaa ctctaaagag 32340
aaagtgaaag cgctgaacgc aatcaaaggt cacggtgctt ctggtgcgga ttgcttcgtt 32400
gttgtatgcc gtcaggttaa tggcctgaaa accttccgtc tgcgtgagcc actggctctt 32460
acttggcagc cggtgcagtt gcacggtctg gtatatgaaa tcccaggtcg cggtcgtttc 32520
gctggtttcc aggtaatgta ccctgctgca atctccatca actcaggtat ctgattggtg 32580
aatggcgggt gaaatatccc gcctttgttt aattcgaata agagaaaaag atgcaactta 32640
aaaacaacct cagcggtaat gtgcagttgt tctatccggt taagaaaaat ggtcaaccgg 32700
atgttgactt cattcacatt cccggtggtg caactgttga gttggatgac aaagtcttcg 32760
aagctctctg caaacctctg acaaccatca aagaacaacg catggttgaa gtagaaattg 32820
aaggcgaagc ggaagttaag atggacaaaa agaatgtcct catcaaagag ttctacgaaa 32880
ccggcgaagt taaaattgtt aacctgttca aagagcagat taagaaaggc gacttcactg 32940
ttgtagagcg tccgtcaatt tccaaagagc agaaactcgc tgtcttgaat cagaacggtg 33000
tttccgttaa agatttgagc gacgagcaga ttgatgctct gtacgacaaa ctggcgtaat 33060
caatgttaac actcactgat ttagttcggc gctatcctgc gatggcgtct ttgacggatg 33120
acagattcgc catcctgcaa gaagattccg tgctaatcat gggtacggat gatggacgct 33180
ggctcaactt ctatgacgca gcacaatctg cattaattgc gcatcgtgca acccagtttg 33240
ggttgggtga tactttggat gaagattcag gcgggatgtc tgctgggcca gtcgttcgaa 33300
cagatgtaga tgatgttcag gtggagtttg ctgaccgtat ttgggacaaa gttccttatc 33360
aggaagcaga cctattctca acggcgtttg gtcaagatta tgtgcgttgg cgtcggatgg 33420
catttgctgg tccgaggttc gcatgattaa tcaaaagctg gcgtttaatc gttttacaac 33480
tgcaaaacac accatcgaac tttacatggt tgggcattac gacggaaaga ataattgggt 33540
tggggaagga tatgccccac ctaagaagtt tcgttgtaca cccattccat atggtgacag 33600
ggattcaggc attggtggcc agaaattggt ggctactgat gttggtgaac gtcaaccagc 33660
atttatgcga attcactcca gaagtcaaat gccgatgaaa tcaataatca ctgtgtacgg 33720
actccgatac aaagtggttg aaattgataa gtacgatgat gcaggattca atgtcgtaat 33780
cgctgcaaaa gttctggaga aataatggat attcaagagc gcgaaattca ggcgatgaaa 33840
gaaatcgtcg atgtgtgtgt gggtattcca ttgttcacat acgaaatgca attgaatgca 33900
ccaaggccgg aaggtcaata tgctgcggtc aaatgtattt caagttctaa tccgggctac 33960
gatgaaactc gcctcattga tgacaatggt gttgagaagt ttcgaactcg tggtattaga 34020
atcctgacat tttatatcct ctttagtcgg gacggtcagg agtatattga tttcgataat 34080
tcattttatc gacctgatgt taatgctaaa ttgcgcgaac atggtttcgc agcattaggc 34140
aaagaggcgc tgaacctcgc aactgttcaa ctggaaacaa actgggaagt aaggaaaggc 34200
attaaaatgc aattcaatgt tcttcgtgaa caactttccg agattggaac catgtccgat 34260
gcagttgttg gtggtaagtt tgttgatggt gaccaagtca tcacgattaa aggataacaa 34320
atgtccattc caatttccga tatcatcaat gttagcattg cggttgcacc aaacgcagtg 34380
gcgactgatg gttacggtcc aatcgtcttt atgacgaaag agtttcaacc tgtaatgggt 34440
gagaatgctc tgcgcatcta taccagcaaa aaagctttgg atgaagattt cccatcaggt 34500
gaaatccaga aagctgcaac tgcttggtac agccagaagc ctacacctaa aactttctgt 34560
gttggctcta tcagtacaac ttccggttct ccagcaacct ccggcaaact tgttggtggc 34620
ggtgctgctg ttctggcaga tttgaaagca atcactgcgg gtggaatgac cctgaacatt 34680
gatggcgccc ctcagacaat ctccgctctt gatttgtctg gtgctgccga tttggctgct 34740
gttgcaactt tagttgaagg tgcgttaaca ggtgttgctg taactcaggc ggctggtgta 34800
ttcacaatta catcaatcac ctctggcatt gcttcggctg tttcaactcc tgctgcaact 34860
ccgttgtcaa cagctctgaa actgacgact gccactggtg caacttcaac tccgggtgtt 34920
gcttcaaccg gtatcacgaa tgaccttgcg aaagcttcac aagctgctgc caagacaaaa 34980
cagttcttct actatgttgc tgtagataag tctcttcgtg aaactcaacc tctgctggat 35040
gctgctgcat ggtgtgaatc tgctggtaaa gtatttggtc tggcttctgc ctctccaaaa 35100
gttctgaccg ctggtgacac taccaactat ttcttcaaag ctaagtctca aaaccttagc 35160
cggacaatct gcgtgtatga tgcctcaaat ggcggcgtgg aatatccaga aatttcaatc 35220
ctcggtcgtg ctgcaactgt caacttcaac gttgccaact ctgcactgat tctggcattc 35280
aaacaaggtc catcaatcac cactgctaac cttgaaccaa ctgaacttgc tgcattgcag 35340
tcttacaacg gcaacgcatt cattgatgtt ggcggaaaca ccatgttcta caatggtgcg 35400
atggcagatg gcacttggtt tgatacaatt cagggcgtcg attggatgac tcagaaagtt 35460
caggtgaatg tgttcaacct gttctatcag tcaacaacca aggttccttg gactgaaacc 35520
ggcgtagcaa tggttaacca gcaaatcact ctggctcttg agctggctgt gaccaatggt 35580
ttaatcgccc caggttacga caacgaaggc gtgttctatc cagacggcta caaagttttc 35640
tcaaccgatt tggctctgtt gcaatctcag aaaggcaaac gtatttggga aggttcttcc 35700
tttattgcga ttggttctgg tgcgctgcaa ggtgcggtta tctctggcaa cttcgtacag 35760
taaggatgtg ctaagtgaag caatattcat tctataatac tgacctgctg attgatggtg 35820
cccgtgttga cgggttcacc gctggtcagg cagttatcac tgcacgtcga aatgtacctc 35880
agcatcttcc agtaatggac gcatatggca aacttgctgt tgcgacaact gctgacttgt 35940
ctggtgtgat tacattccca ctcctgcaaa ccgcggattg gaatgagatt ctgtaccagc 36000
gtgctcaatt gactcaggca actggtttgt ctggcaataa atctctgtgg caaccaattc 36060
agattcaggt tgtcgataag atgggcgaca tgcttgttaa cggcgtcaat ggtgtaatcc 36120
tccaacagcc tgcaatcgtt cgtggtattt cctttacctc aaacctgtgg gcaatctacg 36180
tcgaacgtct ccagattaag actggttctt atccagagat tggcgtttaa gctataagag 36240
agaaacagaa tggcttgtga attactcacg cgagagttta agacttcgga aggagttacc 36300
aaactcttca cgacccgcca actggcggcg agtagtgcac tggacttgca tgttgaactc 36360
attggtaaag tgggaaccac tgtgttccca ctcattgaca acaactacaa tttcggtgac 36420
atcattgcag taatgcgtca ggcagataac aaagttgtta ctgacttgat gaagcgtgta 36480
atctgtaatg caaacattga aggccagcca atcactatgg cgactttcga tatccatttc 36540
aatggcgagc tcatgttagc ttgtcaagta tttgccttcg tgcttgaagc aaactataaa 36600
gcttttttca agctagggct cgaaatgaac gaacagttca aattggcgga ggcggagcgt 36660
ttggaagcgg aacggttggc tatggaagcg agccagaaga atacgactcc gacgacttaa 36720
tcagtcaatt cccggacatt cggtattttc tgcatcgccc aattattgaa gacagttctt 36780
tatgcagact ccacgagttg caagatggaa cctattcatt aatcgacctg ctcctattcc 36840
acgacatttt agatttaaga gttaaattaa atcctccagt tgagcgggaa cagtaatggc 36900
tagcagaaaa gaaatgatgg gcatgggcgt ggatgttctc cgcgctcttg gtcttgaggc 36960
cggattgaat tttgagcccg gactctcgaa gtccaaaatg gttttacagc tggaacagca 37020
tcaggcttcc ggttggatgg acgtcaatag ccaattacta cacgcaagtg cagaaggtgg 37080
ctttgaccac atgcaaggct tcaccggtta tgaagaagaa tccatttccg atgcagcgcg 37140
tgttgcacag tcattagctg gtgcaggata taccgaaact ttccatcata ttatgtctgg 37200
tggtaagtct catcatgtag aagctgttca tggctacatg gcaaaacttg gtgtttcagc 37260
cgatgatgtt tggctgcata tgccaaaacc aaatcctgaa ttacctgcgc aagatttcaa 37320
tttattaaaa ggttatttaa ataaccattg ggatgattac caagacatta tgccaccact 37380
tgctgggcat tatgctggtg atattatggg tgagtatgct acgaataaag gccatgttgg 37440
cgattcgtac aaccaccttt catccatgta tttaaacaaa gctgcttaca acaatccaca 37500
cgcatatgag aaagatgctg cccttgttgc aacgcgtttg gcttcggcga tgggaggtca 37560
attccttgaa gttggttatg ctgctgccac tggtgcaaag gttgggtatc attccatctt 37620
gcctcagctt ggttctgata ttgtgcgtgg tgttcaacat cctcgcgaag ctctcaatgc 37680
tgctggtttg cctcttggct caatgggctc tgctgttggt agtggtcgtg gcgattattc 37740
tcttgtcgct tctctgctgg gacgtgcgga aggacaggat gaagcgtcaa aagcaatccg 37800
aagtgaagta agtagtacgc tgaaaagttt ggcaggctca tataaaggtg gtggcgagtt 37860
tggaatgaat ccacatcgtc atgaaacatc tgagcatgac ttacttctcg attctgcaac 37920
tcgttatgtt ggtattgaag atgcacgttc tggttatgca aaccttgacg acacttcata 37980
caacagaaat aacattgcag caataattga aaacaactat gaccgcatgg atgctcaagc 38040
tgcacaagct ccatctggtg ttgccaatcc tgatttaaga atgccacgtc gttctgagat 38100
tggcactgca tggtctgctg atttaaatga accaaccgac tggaatgctg ctcgtgttcg 38160
tcgtgaaagt gctgccattg caaacttgga tgctgctcat gcaaacttcc gtgatgttgc 38220
tgatggtggt ccaagtggtt caactgttcg attccataat ttccaacaag gttctcagga 38280
atggcacgac ttccgtagtc aatatgacat aactggttcc actgttggct cgatgttggg 38340
tagtaattca tatactcgac cttgggcaga aatgattgac cgcattggat tgcagcgcgg 38400
tgatggaagt aagaacgcat tcacagaaag aatgtttgcg atgggccaca agcgagaaga 38460
tgaagctcgt attcgtgtct cagaaagatt aggtcaagaa atccaacaag ttggctcaat 38520
caccaatgac cagtatccgg gaatgatgta ttctccagat ggtttaattg gcgatgacgc 38580
attgtgggag cataaagctc ctgaacgtgc tggcaagttt gcagatttgc ttgcaggtga 38640
ccatccagac tacatggacc agattcaaat gggcatgttg gtttctggaa ggaaccgaac 38700
tttattctcg cagacaattg gaagtgaaac tcgcgaccaa tggattgacc aagacccaag 38760
ttggtatggc agaaacaaaa ctcgactcga ttctattcgt ggtcgtcttg ctgccggtcg 38820
tgaatttgtt gcaagcaatc cagaccttga ccaagaagcg ttaatcaaag gtgctcgttc 38880
tgtaatgcag ggcgaaggca tctggcgtga tgtttctcaa cgttccaatc gtggtttctc 38940
tgcaactgct ggtactgacg ctgacccatt cattgggcgc ggcggttcaa cctacaatga 39000
cgctgctcaa ccgtcttcaa actatattcc gaactttgtg accagtgccg gtaatggcct 39060
tgtgccagtg ggcggaaatg gggctgagac gggcatggcg cttgccgtca aagaaggcat 39120
actaggtgct caggaggagc taaaacagcg ccacgctcag gctggtgcag cctctggcgg 39180
tgttgctggc ttgcttggct ctgatatgcc agacgccgat tttgccgact caactggctc 39240
cggtggtatt cgtggattct tccaacgaat gtttggtggt ggctcaggcg gcgcaggcgg 39300
caacggtggc ggctctggcg gtgcaagtgg gtggatggat gactttggcg gctcgctcta 39360
tggtggcctc cgtagcggca atttgcgtgg gttgcaaaat ggcacattag atgcaatcga 39420
aagtctgccg atggttggcg gaattcttcg aggaactatt ggagctatgc aagctggtgg 39480
tgaaggcatt gcaacaatgt caaactaccg tggtattgca gaagattcag gcatgacttc 39540
tggtgttgca tttgcagcac agactcaggg tttggaaatg atgggcttga atgcaaatca 39600
agccggtgca ataaatcaaa caattcattc tgtatacaac cgtatgcaaa atggagaccc 39660
tgatgctgct gttcaaattg cagtgcaaac ccgtggattg attactcctg ccgaccttca 39720
aagttctggt ggtgatgcaa ccaagatagc tgcacttatt cgtcaacgtg gcgaagaacg 39780
tggctggagt cagtctcgaa ttgctggcgc tatggaaatg gctggccttg atggtatggc 39840
tcgtgttgct accagaagtg aagcaacgat ggaccaatct cgtcgcctgg ttgatgctcg 39900
ttctgctgaa gatgtttctg gtgcaaattc caattgggaa aatgcacaac gcaatcgtgc 39960
tcttgcttcc acagattatt ctgttcctcg ttattcattc gaaagtttgg gtggtgctgg 40020
tgagagttta tctggaagta tggtgcaggc tgcggcgggt gctgaacgtg ccttccgtgc 40080
tggcggagac atgattgact tggttcgcaa tctggaatca ggtggtcaag acttcgatgc 40140
tcatggtaat ccagttactt catcgtctgg tgcgaagtac tcaatgcagg ttcttccttc 40200
tacagcccgt gacccaggtt acggcattcg tccagcggca aatgattctc cagaagaata 40260
taaccgtgtt ggtcgtgaga agttagatgc aatgatgaaa cgttacggtg gcgattggcg 40320
taaagctaat gctgcataca ccgatggcga aggaactgtt gatagtgcaa tcaagcaata 40380
tggaaacgat tggttgagtc atatgccgtc tcaagcacag aaacgtgtaa gagacatgga 40440
acgtgctgga ttcaatggtg gtaacgcaaa ttcattcagg gatgctggct caaatggtca 40500
acagaatctg aatgtaactg tgaacatcaa tgcaactgtt aatgacaaaa gcactcgtgc 40560
ttctgttact aacccgtaca gtggtgaagt tgttacaatc aactcaaatc acggttctgt 40620
tgcacagcgt cgatgagatt tgtgcggggc ttcggctccg cgctttctta aactttattt 40680
gaaattgatt tatgaaggtg tcattgtgtg gattagacaa gtacaggtta tcgttacaaa 40740
cgcacagaat ccaaaaataa aaactgtatt tgaaaaacac agtattgaat ttgaggtgcg 40800
ttcgacttta ggttgggctg ctgatacagc aacagtaaca atcaagaact tgtctttgcc 40860
agaaattaaa ttcttgcaga ataaaacctt tggcgacctc ttgattgaaa tacgtgctgg 40920
atggcttgat gagttgcaag gctcaagtgt gaaacaacaa tgggttgcac aagggcaaaa 40980
tgctctggtg acaatagaag agaataaaaa tcttccaacg ttgttctctg gcattctgtc 41040
aaatgctgtg gggtataaga aagctcccga gcatataaca actttattct gtgtatcaaa 41100
cgcaggtgca gttgctacat cgtttaagca aatgcgggat attcctgcgg gttcaacatt 41160
gaacgatgca atcaagtcta tgtgtactga ttatggatat aataccgttt cctcttttgg 41220
tgtgtctgga acaacactca caacaaaact tcctatggga cgtgtattcc atgatacctt 41280
cataaaagaa tttggtaatt tgctcgaaga atacaattta aatttctata tcgccacatc 41340
tgagattcaa atattctcag acacgtatgg tgattctgat gccatgaagc gaatggctaa 41400
agatagagag ccagttaaaa ttgatgccaa ctcagttatt ggaactccaa tagcgggcat 41460
tggtattttc gatttggcaa tgtatcttcg aagcgatatt cagcccggaa tggttatgga 41520
tgtttctcca ttactcggaa cggaattgct ggcaaatggt gtaacaagtg tggccggtcg 41580
tggtcaggca ctgaaccgtg ttgactcttt attccagtac gcaatggaag acaagtatca 41640
aatcataacg gtgatacaca gaggtttcac acatgaccaa gacttccaaa ccataattca 41700
tggtgttctc ggtggaaaca ctgcatcagg tttgtcagag caaaactggc agaactggta 41760
cattggaagt ggcatggcgt cagcacacta gaggttctaa tgtcagatat aattggtgat 41820
gttggcacag ctttgagcgg tgctgcatcc aaattaaaaa gcaagttcag ttacggaccg 41880
catccatcaa tcatcatgtg ggcggctggt cctgcaactc aagttcagga taaagatgat 41940
tctattcttg gttctattgg cgaagctatt tcgggtgcca gaaccaagct ggagaacatc 42000
gtaacatcta caccagaccc aagtcagttt aattcattta aatttgatgc gatggttagt 42060
gagtctcatc ttgctcaatc aacggtgaca aaggttcctg tgtctaccgg gtttgttgtg 42120
tctgaacaca tcatcaatca taaccgcatt ctcaaacttg aagcagttgc aacaaacatg 42180
cagaactctg caatgtgggc tgtgtctgct caaggtttat ctgtggttag tggtgcaatc 42240
ttcaacaatc caatcctccc tcaaattggt ggattgtatg gtgtggtatc ttctgctttt 42300
gaaacagaaa acagaattga atccacctac aatttattta atgggttaag gacttccggc 42360
accaagttgt atatatcaac cattcttgga aagtatttga actgtgtaat aacagaaata 42420
cgaaccaagc atgacaagct cactgcctca atgctggcgg ttgagattac gctggaagag 42480
ttgcaaacaa ttggtaagga tgacattgct aaagctgcgg ctactgcaat cgacaactcc 42540
tacgattatt ctgagtttgc caaagttgca acagggcttg gtgttggtgt acttggtgga 42600
gttcctttac cgggacttgg ttccttgtcg aacccaactg cacagctcgg tgttttgaag 42660
gacaagcttg ctaaattgtc tcaacctatc tcagcaatca agggaaggat attatgacag 42720
taaaggaaga acaacaagcc ttcaacgaaa tcatgagttt gttgcctcct gggtatgtta 42780
agaaaattcc tacatctgtg acaaaggata tttcattcca gtttaatggt ttgagtattt 42840
caatgtctgc actgtatctc aatacttttc ttaattgtta ttgttttgat ttggcatggt 42900
cttccacgga taaaatctat ggcatacctc tcagatgtgg tgttgatatt ttaaaacaat 42960
actcgacacc acttccaaat ctttatgcga acaatcatgc gtatcccgga agcgaagtga 43020
cgagttggag aaatctcgac ttaatcatta ttgacgaatc ggtgcttgaa cgtgggtagt 43080
caaaataaca ctcgtgctcc agatataaat acaggttatc cgggatttgc ttacaacttt 43140
gatgccgaca cacaaacctg tgaagtgcaa cttgcaattg aaaatttgtt tatcggaatg 43200
caacagccat atgctcttgt tccaaaaagc agattaaaga atgtaacggt tcaattcacg 43260
caaggtggtg gttggagttt aacgcatccg gttcctgacg gtacgccagt gtgggttcat 43320
ttcgctcagc gaggaattgc acactggcaa gtcgaaggca aagacagtgc aggattaata 43380
aatggaaaac ctgctcctgc atttagccag ttgtattcaa tcaacgcggc gtcatgtact 43440
gtcgggaacc aacctattcc gaaagcaata aaagctttta ataatggtgt tgctgagttt 43500
cgaaatgctg atagaagtca gcgtgtaact ttgaatgaaa gtggtggcat taacattgtc 43560
gccaattcag caaaaatcat tgttaataag gatggtacga ttgagctcga ctctccgaca 43620
gaagcgattg ttaaggcacc gaagattaca ttggatggcg acacaacagt caccaaatcc 43680
ctcaccgttc aaggtggagc atcaatctcc ggtggtacag gtcaaacctt caaagtaacc 43740
ggtaatgttg agcatcttgg ttcttattct ctgaatggaa tcaaagtaga tggtcataac 43800
cacactaacc cggaaggcgg aaacgtcggc ccaatgaaga atggataaat gtccggaaac 43860
ctaaagctag atgcaaacca cgatatcata attggtcgtg gaacaactcg cgttggtggg 43920
gttgaatatg tcgcacaatt ggttaaatgc cggttgctga cattgcttgg cgaatggcaa 43980
caagacagca ctctcggact tccttggtat gaagtaatat ttactaaaga tgttcgggat 44040
tctgatattc aagttgctgt agcaaatata attcgctcca ccaaccatgt gcgtcaactt 44100
ctttcgatag atattgatgc agataggaaa gcccgaatct taacaattaa ctttgtagcc 44160
atttctgatt atggcaatat ctcagaggtt gtggaatggc gacagtaacc tatggtgtaa 44220
cggataaggg atttgtccgc aagccgcttg aagcaatctt ggatgatttg aactctgcgt 44280
tcaaaggcgc ttacggctca actttcgata cttcaccaga atcaccagac ggtcaagcta 44340
ttggcattat tgctgataga atttctcaat gttgggaaca ggcacaatat agtttcaatg 44400
cctatcgtcc cggtgctgtt gaaggaactg gtttagataa tgtgtgtgaa ttaaccaaca 44460
caataaggta tgtaaacaaa ccaactcagg tcactgtaat gtgcactggt gcaaatggtt 44520
ctgtggtgcc tgctggacta gttgttgggg acgactcagg tcacacattt aaaaccaaaa 44580
ttgatgtaac ccttccggga gacgtaacag catattgcga gcaacagggt gaaatctatg 44640
ttgcagcaaa cactgttact aaaattgtct ctggcggaaa tgtttcactg acttctgtga 44700
caaacccgga agaaggtcag actggtattg actatgagaa agacccagca ctccgttctc 44760
gccgtgacaa aacaacggtt ggttcaggaa ctgcgacagc ggaagcaata tattcagcac 44820
tcgctgacct caatcttgat tacattcgta ttcgtgataa tgataccgat gttgctattg 44880
gttctcagcc agcaaatact atttgggtgg tagttgatgg tggcacggtg aacgatattg 44940
ctcgccgcat ctacgacaac aaagctggtg gtgttcctac ctatggtgat gttgtttcaa 45000
cattcaagga cagtaagggt tatccaaaga caatcaagtt tagccgcact tccaaagttg 45060
cgactttctt tgatattaaa gtcaaaagat tgcctactgc taacttgagt tctaacgatg 45120
ttgttgttgc tgttcaggat gctgttcaga attacattga cagtttgcag cccggtgctc 45180
cagttgtttg gtcttatctg attccgccaa ttcttgcgtc tgttcaaggc attcaggtag 45240
atgacttgca ggttggatta actgctgcga atgttgcaca tacaacgctc gaaatggata 45300
tcaaccagcg tgccagcact gcaaccgcga acatcaaagt tacggacacc acagttccat 45360
aaggattaag atggccgaag ttaaacatgg catggacttg ttactttctc agtacaagaa 45420
cagcccaaac ctcaagaagt atattcagtg cttcttagat gaacttgatg ttgtttcatc 45480
ggctatgtct gattctgtta agtatagata tcttgcagac tcctttgggg ttatggttga 45540
tgatattgct tacatagttg gtgcaagccg tactttgtat ggtgcggctg cacttgggtt 45600
ctttggttac tatgagaacc ctgctgcgga atcaactggt gatgataaca accctcttgt 45660
tggtggtatt ctgcgttccg attctgaccg gagttctggc gattttgttc gcactgatgc 45720
tcagctgaag aatgccatta gagcacgcat tgttaagatt gtatccaatt gctgcattga 45780
agacttaatt gtattttgtg atttggtctt gggtagggaa ttggatatgg agattgtgga 45840
aggcccgttg aagctggaat tcaaagttca tgaaacttta ccaactgccg accgtgttct 45900
tttgtctttc atgattcctg atataaaacc tgctggcatt acagccaagc tggaagatga 45960
ttcaggcaac attgcgttag tctactattc aaaagattat ccagcgagag tgatatgaca 46020
aatcgagttg gtgatttaag tcagatttgg gcatctaacg gggaagcaac agaccccgat 46080
ttagatacca cgcatccgat attccagccc aataaatata ttaaaggctg gaaggtagag 46140
aaggagcctc accaatggca aaacttcttg taccaaatct ctgatgaaaa attgttgttg 46200
gttgctcagg aacaaatctt ggagtgggaa tctgatattg gctatgccaa aaatggtatc 46260
accagaaaag gagacaaact ctacaagaat gttagtggtg cagtttccac tggtaaagac 46320
ccagccacag aaacatctgt gtggaacgaa gttctgagca tggactctgc tggtgttgtt 46380
acagccgtaa ccactctgac aaacacactg aatgctcact tggctgccga caatcctcac 46440
aaagataaca tccatgatat tggtggatat gagtctggtg agattgataa attctttggc 46500
gacccgaaca accctgcaac aatcgtttac cacgaaagtc agacaggagg taaagttcat 46560
ggcgaaactc cagcgcaagt tggtaccttg cctaccagtg gcggagcctt tactggtgat 46620
gttaattaca ttggtggttt aacaataggt acgggaagtt tccgaattga tggaccagac 46680
cagaagatta caaattccgg tggttcgcta ttccttgaag gtgatggcga tacaactgtt 46740
ggtgttgagt cttctggtgt tcgcagtgat ataacaactg ttgcgaactt cgatgcacat 46800
caaattgcag taaataattc ctttgcactt cctcaaccga ttctgcaaat gcagcttcgt 46860
gctggttctt ttgacacaat gggtgtcggt aacttcattg ttgaaacaac cgatgctcca 46920
gtgttcgaag ggaataaggg atggaagatt gccgataata cattcatgac ttgggtggga 46980
ttagttagta ttcctgcaac aatttacttt gaatactatg ctggcgatat caggaagttt 47040
gctgttgttg atgccacaca agttgtcgtc aaaccaaatc tgaaaatatt cctgcaagca 47100
ttgattcctg atgcaacgca tttgtgtgtt atggatattt atcttgcact gactgaatac 47160
cagaagttga acctgaaacc gaagtatgat tctttgcata ttgcaaatga tacatcttca 47220
actccgggaa cagcttggta tgtttccaaa gtcacgacag atgttttcgt aatgcctaat 47280
gcaactgctc ccaaaaatgg tcagtatatt ggtgcattcc gttggagtcc tgacgatgtt 47340
cgtggtgctc gattggatgg cagtgctgtg tggacaactt ccgattcaag tgttgttcag 47400
attgttggtt caacacaagc aaatggtcag gctgtaatca acatcctgaa agcaggaact 47460
gcaaccttaa ctgttaaatg gaacggaatg gctgcaattt taactatcac tgctgtatag 47520
gaatagataa tgctgcgtcc taaacttggc cgaatttggg gtgactcgaa ttcggttgct 47580
cgtcgtgacc caggcgacga aaaatattta aaaggttggc tgtctgaaat cccaacatac 47640
caagttctga actatcttca atggaagacg gattcaacac tgcaagctct tactgaacgt 47700
ggtgtgtttg aatggggtgg cgatgcaacc taccgaaatg gtgctgcggt ttgggatgaa 47760
tcaaatggag tgatttatat tgccaaactt gatggtccaa gcaagacgct gaaaccaagt 47820
tcaaacttgg ctcaatggac tccaagtgca ttgcagatta ctcgtgcaga ttatgatgca 47880
atttctgcgg caatcgcaag tcacatcgct gatgtaacaa gtaaccctca tggactgact 47940
ccgggaagac ttggaacata caccactgca caaattgatg cattggttgc tcagtacaag 48000
aaaatggttt cggaccatgc agcaaggaaa gataacccac atggagttac agccactgac 48060
attggcgcag ttcctgtaac tggtggtgca tacaccggca atgttgaaat gaagactggt 48120
caagttcttc tgtctgccga tggtgtacgt aaaatcaaag ctgatgacac tggcgtctat 48180
atggcgaaca gtgcaggtca agttggtgtt gatgcttctg gtgaaggttt tgtcaaaact 48240
gggaatgctc cagcaaccaa aatcattact aaagcaacat ttgcagataa taaagctgct 48300
gttgaagcag cctatgcagt gcagcaacct gtgttctatt tgcctcttgc atctgatttg 48360
aatgtctaca ttggcggtgg tactgttcag gccgaccaaa atggtggtgc actggattgg 48420
aatccatctc tactgaatgg tgtaatcttt ggaaacaata cacaatatga caaccgtgtt 48480
gtgtatacat acaaacctct tgaaggtttg aaagaagcaa ccatttgtgt tgatgttttc 48540
cttgaaggaa ctggcggaac tacaactgct cctgaattgt atgcatcatt tggtgctgct 48600
gctggttgtg gtgttattca agttaataag aataacacag ttcgtttctg gtctgagact 48660
ggaacaactg gaacaatccc acttccaacc agtgtctggc ttcgtcttgt tggtgtaaga 48720
actacaactt ctttgtcgct gtatctcaac ggagttctgg ttagtacagt tgcagccaac 48780
aatgcaaaca tcacaggaag tgattacaac ttcctccgtg cagatgttca ggcacaagca 48840
aaccagagac tgattaaagc caaaaacttt aaactctgga ttggtgcttt gacggcaaaa 48900
caaatctcaa cgctataagg gttaaaaatg gccaaaggtt ggtctttcgg cgcacttaca 48960
gctgcgttaa aaggtgctgc atttcttgat gttggcacca ctgcaaacac tgtcgctgct 49020
ggtaatgact cacgaattgt caatgctttg cagaagaaca acaacttgac tgatgttgcc 49080
gacaggactg ttgcaagggg tagtttgtat gctgcctaca taaacggcga tgcaagtcaa 49140
gcattccagt gttcgaacag tggttctgat aactatgctg taaacaatgt tcgactcaac 49200
ttcatgctgc aaagtaaggc tgctgtaaat ggcaaccagt acacacagtt tgatgttggt 49260
ggtgcaacaa gtggtgcaca tgctgtacgt ctagaccaat tccagtctgg tacaaacggt 49320
aatggtgctt ggactaagat tccaaatggt cctatgtggt gtagacagaa cttgtctctt 49380
gcagcaaatg caaccacaac ttggaatttc ccggcgcaat tccctggaag tcctgctgta 49440
ttcttctcag ctttcaatgg tgagaacagg gcgtggttta acggcatttc tcaaaatggt 49500
tgtaacatct acaatccaca gaatgtcgcg gtcaacgtta acgtgtatgc gatatggtga 49560
ttgataaggg ctacaaagtg gatagacaaa ctatccaagt aatcttccta ttcatgcaac 49620
agtatggatt actgcatggt ctgttagctg gacttactgc tttaattcgt ggagcctatg 49680
agagtgaagg tcttggaaaa gctctgcttg atgcagcgct gtgttgtgtt attggaacgt 49740
ttgtcttctc actcccaatt ctggatggtt atttggttaa tcatcctaat gcaggatttg 49800
ttgtttgcat tgtgattggt gtagtaggtg cgaagttaat tatcactact ttacgtgatt 49860
cgttctcggc agcaattaag caattgaatc cattaacttg gttcagaaaa gcaagataaa 49920
aggaacacag atgcggaagt tgttttgcat catgctcctg atatttgcgt ggaacacttc 49980
ggcattggca actgctgatg ctggagtgaa ttccaaatgg aatgcgcatg tgtcggagtt 50040
tgcagctcaa tcttatctcc aaggtggatt gaaattaact gcaagtcaga aacatgaagt 50100
tgactgtttg gctaaagcta tcttcttcga agccagagga gaaagctatg ctggaaaggt 50160
catggttgca aatgtcgtac tgaacagagc taagtttggt aagccatttt caaacaccat 50220
ctgtggtgta atttatcagc cacgccagtt ttcatggact tcaaacaaat ggaagcggaa 50280
cacttctttt agagaagtag cgcggaaatt tgactcaaaa gagcacaaag ctgtggtaga 50340
aagtcttgaa attgcattga aatttgtgat tttagacacg aaaaacacag gaaaatgtac 50400
tcatttttca tcaaaatctg acaaatttgg acgtactttg ttcataaaac aggtcggaaa 50460
tcacaaattt tatcaatatt taggtaacgg ataatggata aagctctaat gcaatacttg 50520
gcaaatcttg ccgaagaaca ccattgcact attatcttcc ttgataaatc aatctggact 50580
cctgaaaagt tgattcaaat gcaggaagcc ttgctcaaga ttatcgacga agatgccgaa 50640
attaaggtgc attgaatctt gtcagttaga tgttgaggga aggtggtcgc gaaagcgaac 50700
atcctttccg agactattaa gaaagaaata agttaacaat attcaattcc aaatattgct 50760
acaatattat gcatgtgata tttgtaatgg gatatgcgat ggatggtcgg tcagctgtac 50820
ttcatctagc tggcttaaat ggtcagtcgg ctgtaccaaa ttaaattcga ttcgagattc 50880
gcttatgatt cgaaatttag attcggatta agattcaaat tttaatatta attttttttt 50940
attggagcca atggtgccgg attcgaatac gaattttata aaaaattttt ttaagacgta 51000
gaccgggagc ccaacaccgt cctatcggac ttaaaatacg gtcccttaac ttctttttcc 51060
aattcgtcct atcggactca aatcaacacc aaagtagctc ctgcttcgca ggcataacaa 51120
cgcatctaca tcagctccct taggtatcaa tagtgtaatg gctacgcaaa ctaaatccgt 51180
ttcatccgac aacggcactg gtttgtgggc ttagttgact atgtataaaa ccttggagct 51240
atatcatgac taattcaaac gttgttaaat ctgttgttcc ttctatcact tctgctatct 51300
tccgtgctaa agaaacacag agctacatgc agccacagac ttgtgctatt gccgctctcg 51360
ctatcttcca catgatgacg tcattcacac gtgacgatgt tgtaccagcc gttgcgatgg 51420
ttagccttgc agaagcgtca atcaagaaag ccaactctac caacaatgac ctgaaacagt 51480
tccactatgt tgacccatct atcaacttga ttggactggc taagcatctt ggctatgtat 51540
caattgcaga agataagtca ttcaccatga ctgagcgttg gattgaactc gtttcaacta 51600
aagagacttg cactccactg acagaagctg tgtcagaact caatcgtcgc aagccattca 51660
tcaaaggtgg caaacgtaag ccttccaaac tgatgacaga atgtatcgac ttcctgcaat 51720
caactgaata ccatgttgat gcagccatgg ttgacattgt tggtgacgta ctcggcatgt 51780
ctcatggtga cattcctgat gttatcaaac aagagttgca tgtttggaac aacgctaaga 51840
acatggcgaa agaagatgtt ctgttctctg attactttgc agataaccgt ggtcgtttgt 51900
atcacgttgc atgtgctggt ccaaatccac agtcttctga ctttgctcgt tcactctatt 51960
ctcacaacat cgagaacatt gtgaagaaag acagtgcagc ataccagatg ttcatggctg 52020
aattgaatga catttctggt ggcaagtgga cagaagctcg catgttaact cgtgttgctc 52080
agaacccagt tggagctctt gctcacatgt tacgttctgg tgacgctcct aagaagccat 52140
tcacttacat tcgtctggca ttggattggt tcaagtttga aacaactggt gagtgtgatt 52200
cacgtgttgg ctttggctta gatgcaaaat gttctggtac tcagtatctg gcattcattg 52260
caggcaacat gcaaatggca caggcaactg gattagttgg ctcagattct aaagcagctg 52320
acccatatca attgtcgctg gtggaattgt tgaaactgtt agacaagtct tcaatgaaac 52380
ctaccgatga agtaaaagag aaatatttca atcgcaaaga tggacgtaac ttcatcaaga 52440
ctccatacat ggctgttcaa tatggtggtg gcaagaaagc tctgacaggt aacaaagact 52500
tcgtagcaac tgttactgaa atctttggtg cagataaagt tgaagcattt gctgaacttg 52560
ctgttgaagc tgtgaaagct gcacttggtg aaaagattaa catgttcatc gctaaagtga 52620
tggaagctgt taaagccaaa tgtgagaaag aatctaaact ctacattgat tatcgccaca 52680
ctgatggaca agtggttcac aaaccttgct acccatcacg tgaaatctgt gacccattct 52740
cgattcgtgt tgactctaca actcgtgtca tctttggaca acagaatgaa ggcaaagctt 52800
ggagcattcg tgagacacag ccaactgtag aagaatttgt tcgtaccttt gttgttaact 52860
acattcaagg tattgatgca ttggttgcac gtactgttgc caagtatgca aagaaagctg 52920
gacttcgtgg tttcacttca attcacgatt gcttccgttg ctgcttggca gatgctccta 52980
agatgatgga tgtaattcgt caggcatatg ttgaaatctt tgtgaagaat aatcaattcg 53040
aagcattgag caaacaaatt ggtggcattg atatgttcca taccaatatc gtaacagaag 53100
aactcctgat gagcgaacac gcttattact tctgccaata atccaaaaca actaagggct 53160
tcttcggagg ccctttcttt attcttttaa aaaataaaat cttttaatcc caatattgtt 53220
ttaatattcg agacaacatt ggcattagtt ggaggtggag taacaaaatt ctgcatgtca 53280
agttcgaaac tggtgtacaa cagtattgag catgtcagct tagtcacagt gatgttacag 53340
gtttagtcaa ctgtagaact ccctgagatg gctactcaaa gttccagcat ccatggctga 53400
gtaagaactt gttgttccga gaagagattg tccaaatgtt ccatgtgagt tactgagttc 53460
cgtaatccta ttataaaggc cctaacacaa gccgggattg aattggaact tgctggctca 53520
aatggaatag tggtgacaaa atcgagaagt gagtggcat 53559
Claims (19)
- 에르위니아 아밀로보라(Erwinia amylovora)에 특이적인 용균활성을 갖는, 기탁번호 KACC 97037P인 박테리오파지.
- 제1항에 있어서, 상기 박테리오파지는 서열번호 1의 염기서열을 갖는 것인 박테리오파지.
- 제1항에 있어서, 상기 박테리오파지는 미오비리대 과(Myoviridae family)인 것인 박테리오파지.
- 제1항에 있어서, 상기 박테리오파지는 20℃ 내지 60℃에서 활성을 갖는 것인 박테리오파지.
- 제4항에 있어서, 상기 박테리오파지는 25℃ 내지 50℃에서 활성을 갖는 것인 박테리오파지.
- 제1항에 있어서, 상기 박테리오파지는 pH 3 내지 12에서 활성을 갖는 것인 박테리오파지.
- 제6항에 있어서, 상기 박테리오파지는 pH 4 내지 11에서 활성을 갖는 것인 박테리오파지.
- 제1항의 박테리오파지를 유효성분으로 포함하는 농약 조성물.
- 제8항에 있어서, 상기 박테리오파지는 과수 화상병(fire blight)의 예방 또는 치료에 사용되는 것인 농약 조성물.
- 제8항에 있어서, 상기 농약 조성물은 부형제를 더 포함하는 것인, 농약 조성물.
- 제10항에 있어서, 상기 부형제는 계면활성제 또는 증량제인 것인, 농약 조성물.
- 제8항에 있어서, 상기 농약 조성물은 수화제, 입상수화제, 액상수화제, 액제, 수용제, 수용성입제 및 캡슐화제로 이루어진 군에서 선택된 하나 이상의 형태인 것인, 농약 조성물.
- 제1항의 박테리오파지를 유효성분으로 포함하는 항생용 조성물.
- 제1항의 박테리오파지를 유효성분으로 포함하는 소독제.
- 제1항의 박테리오파지를 유효성분으로 포함하는 세척제.
- 제1항의 박테리오파지를 사용하여 과수 화상병을 방제하는 방법.
- 제17항에 있어서, 상기 과수 화상병은 에르위니아 아밀로보라 (Erwinia amylovora)에 의해 유발되는 것인, 과수 화상병을 방제하는 방법.
- 제1항에 따른 박테리오파지의, 과수 화상병(fire blight)의 예방 또는 치료에 사용하기 위한 용도.
- 제1항에 따른 박테리오파지의, 과수 화상병(fire blight)의 예방 또는 치료용 농약 조성물의 제조에 사용하기 위한 용도.
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