KR20230084505A - Dna 변형 효소 및 그의 활성 단편 및 변이체 및 사용 방법 - Google Patents
Dna 변형 효소 및 그의 활성 단편 및 변이체 및 사용 방법 Download PDFInfo
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- C07K2319/81—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor containing a Zn-finger domain for DNA binding
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Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202063077089P | 2020-09-11 | 2020-09-11 | |
| US63/077,089 | 2020-09-11 | ||
| US202163146840P | 2021-02-08 | 2021-02-08 | |
| US63/146,840 | 2021-02-08 | ||
| PCT/US2021/049853 WO2022056254A2 (en) | 2020-09-11 | 2021-09-10 | Dna modifying enzymes and active fragments and variants thereof and methods of use |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| KR20230084505A true KR20230084505A (ko) | 2023-06-13 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| KR1020237012126A Pending KR20230084505A (ko) | 2020-09-11 | 2021-09-10 | Dna 변형 효소 및 그의 활성 단편 및 변이체 및 사용 방법 |
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| US (9) | US12398384B2 (https=) |
| EP (1) | EP4211235A2 (https=) |
| JP (2) | JP7719172B2 (https=) |
| KR (1) | KR20230084505A (https=) |
| AU (1) | AU2021339805A1 (https=) |
| CA (1) | CA3173886A1 (https=) |
| IL (1) | IL301139A (https=) |
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| TW (1) | TWI910226B (https=) |
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Families Citing this family (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2019265019B2 (en) | 2018-05-11 | 2025-11-06 | Beam Therapeutics Inc. | Methods of substituting pathogenic amino acids using programmable base editor systems |
| EP3847254A4 (en) | 2018-09-07 | 2022-08-10 | Beam Therapeutics Inc. | COMPOSITIONS AND METHODS FOR DELIVERING A NUCLEOBASE EDITING SYSTEM |
| WO2020051562A2 (en) | 2018-09-07 | 2020-03-12 | Beam Therapeutics Inc. | Compositions and methods for improving base editing |
| AU2020215730A1 (en) | 2019-01-31 | 2021-07-29 | Beam Therapeutics Inc. | Nucleobase editors having reduced non-target deamination and assays for characterizing nucleobase editors |
| CN120174005A (zh) | 2019-02-13 | 2025-06-20 | 比姆医疗股份有限公司 | 具有用于修饰靶标序列中核碱基的腺苷脱氨酶碱基编辑器的经修饰的免疫细胞 |
| WO2020191233A1 (en) | 2019-03-19 | 2020-09-24 | The Broad Institute, Inc. | Methods and compositions for editing nucleotide sequences |
| US12473543B2 (en) | 2019-04-17 | 2025-11-18 | The Broad Institute, Inc. | Adenine base editors with reduced off-target effects |
| EP4034138A4 (en) | 2019-09-27 | 2024-07-31 | Beam Therapeutics, Inc. | COMPOSITIONS AND METHODS FOR THE TREATMENT OF LIQUID CANCERS |
| CA3196831A1 (en) | 2020-09-25 | 2022-03-31 | Beam Therapeutics Inc. | Fratricide resistant modified immune cells and methods of using the same |
| EP4352215A1 (en) | 2021-06-11 | 2024-04-17 | LifeEDIT Therapeutics, Inc. | Rna polymerase iii promoters and methods of use |
| CN114634923B (zh) * | 2022-04-07 | 2024-02-23 | 尧唐(上海)生物科技有限公司 | 腺苷脱氨酶、碱基编辑器融合蛋白、碱基编辑器系统及用途 |
| CN114686456B (zh) * | 2022-05-10 | 2023-02-17 | 中山大学 | 基于双分子脱氨酶互补的碱基编辑系统及其应用 |
| EP4569093A1 (en) | 2022-08-12 | 2025-06-18 | Life Edit Therapeutics, Inc. | Rna-guided nucleases and active fragments and variants thereof and methods of use |
| CA3265664A1 (en) | 2022-08-25 | 2024-02-29 | Life Edit Therapeutics, Inc. | CHEMICAL MODIFICATION OF GUIDE RNA WITH A LOCKED NUCLEIC ACID FOR RNA-GUIDED NUCLEASE-MEDIATED GENE EDITING |
| KR20250126155A (ko) | 2022-11-04 | 2025-08-22 | 라이프 에디트 테라퓨틱스, 인크. | 내부 삽입 부위를 갖는 진화된 아데닌 데아미나제 및 rna-가이드 뉴클레아제 융합 단백질 및 사용 방법 |
| EP4634383A1 (en) | 2022-12-16 | 2025-10-22 | Life Edit Therapeutics, Inc. | Guide rnas that target foxp3 gene and methods of use |
| WO2024127370A1 (en) | 2022-12-16 | 2024-06-20 | LifeEDIT Therapeutics, Inc. | Guide rnas that target trac gene and methods of use |
| WO2024178397A2 (en) | 2023-02-24 | 2024-08-29 | Elevatebio Technologies, Inc. | Modified immune effector cells and methods of use |
| CN117965505A (zh) * | 2023-06-28 | 2024-05-03 | 微光基因(苏州)有限公司 | 工程化的腺苷脱氨酶及碱基编辑器 |
| WO2025022367A2 (en) | 2023-07-27 | 2025-01-30 | Life Edit Therapeutics, Inc. | Rna-guided nucleases and active fragments and variants thereof and methods of use |
| WO2025083619A1 (en) | 2023-10-18 | 2025-04-24 | Life Edit Therapeutics, Inc. | Rna-guided nucleases and acive fragments and variants thereof and methods of use |
| WO2025122725A1 (en) * | 2023-12-06 | 2025-06-12 | The Broad Institute, Inc. | Methods and compositions for base editing of tpp1 in the treatment of batten disease |
| CN117511912B (zh) * | 2023-12-22 | 2024-03-29 | 辉大(上海)生物科技有限公司 | IscB多肽、包含其的系统及其用途 |
| WO2025174908A1 (en) | 2024-02-12 | 2025-08-21 | Life Edit Therapeutics, Inc. | Novel rna-guided nucleases and proteins for polymerase editing |
| WO2026003754A1 (en) | 2024-06-25 | 2026-01-02 | Life Edit Therapeutics, Inc. | Novel reverse transcriptases and uses thereof |
Family Cites Families (58)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4217344A (en) | 1976-06-23 | 1980-08-12 | L'oreal | Compositions containing aqueous dispersions of lipid spheres |
| US4196265A (en) | 1977-06-15 | 1980-04-01 | The Wistar Institute | Method of producing antibodies |
| US4235871A (en) | 1978-02-24 | 1980-11-25 | Papahadjopoulos Demetrios P | Method of encapsulating biologically active materials in lipid vesicles |
| US4186183A (en) | 1978-03-29 | 1980-01-29 | The United States Of America As Represented By The Secretary Of The Army | Liposome carriers in chemotherapy of leishmaniasis |
| US4261975A (en) | 1979-09-19 | 1981-04-14 | Merck & Co., Inc. | Viral liposome particle |
| US4485054A (en) | 1982-10-04 | 1984-11-27 | Lipoderm Pharmaceuticals Limited | Method of encapsulating biologically active materials in multilamellar lipid vesicles (MLV) |
| US4501728A (en) | 1983-01-06 | 1985-02-26 | Technology Unlimited, Inc. | Masking of liposomes from RES recognition |
| US5380831A (en) | 1986-04-04 | 1995-01-10 | Mycogen Plant Science, Inc. | Synthetic insecticidal crystal protein gene |
| US5049386A (en) | 1985-01-07 | 1991-09-17 | Syntex (U.S.A.) Inc. | N-ω,(ω-1)-dialkyloxy)- and N-(ω,(ω-1)-dialkenyloxy)Alk-1-YL-N,N,N-tetrasubstituted ammonium lipids and uses therefor |
| US4946787A (en) | 1985-01-07 | 1990-08-07 | Syntex (U.S.A.) Inc. | N-(ω,(ω-1)-dialkyloxy)- and N-(ω,(ω-1)-dialkenyloxy)-alk-1-yl-N,N,N-tetrasubstituted ammonium lipids and uses therefor |
| US4897355A (en) | 1985-01-07 | 1990-01-30 | Syntex (U.S.A.) Inc. | N[ω,(ω-1)-dialkyloxy]- and N-[ω,(ω-1)-dialkenyloxy]-alk-1-yl-N,N,N-tetrasubstituted ammonium lipids and uses therefor |
| US4797368A (en) | 1985-03-15 | 1989-01-10 | The United States Of America As Represented By The Department Of Health And Human Services | Adeno-associated virus as eukaryotic expression vector |
| US5569597A (en) | 1985-05-13 | 1996-10-29 | Ciba Geigy Corp. | Methods of inserting viral DNA into plant material |
| US4774085A (en) | 1985-07-09 | 1988-09-27 | 501 Board of Regents, Univ. of Texas | Pharmaceutical administration systems containing a mixture of immunomodulators |
| US4853331A (en) | 1985-08-16 | 1989-08-01 | Mycogen Corporation | Cloning and expression of Bacillus thuringiensis toxin gene toxic to beetles of the order Coleoptera |
| US5268463A (en) | 1986-11-11 | 1993-12-07 | Jefferson Richard A | Plant promoter α-glucuronidase gene construct |
| US5608142A (en) | 1986-12-03 | 1997-03-04 | Agracetus, Inc. | Insecticidal cotton plants |
| US4837028A (en) | 1986-12-24 | 1989-06-06 | Liposome Technology, Inc. | Liposomes with enhanced circulation time |
| US5039523A (en) | 1988-10-27 | 1991-08-13 | Mycogen Corporation | Novel Bacillus thuringiensis isolate denoted B.t. PS81F, active against lepidopteran pests, and a gene encoding a lepidopteran-active toxin |
| EP0388186A1 (en) | 1989-03-17 | 1990-09-19 | E.I. Du Pont De Nemours And Company | External regulation of gene expression |
| ATE225853T1 (de) | 1990-04-12 | 2002-10-15 | Syngenta Participations Ag | Gewebe-spezifische promotoren |
| US5264618A (en) | 1990-04-19 | 1993-11-23 | Vical, Inc. | Cationic lipids for intracellular delivery of biologically active molecules |
| WO1991017424A1 (en) | 1990-05-03 | 1991-11-14 | Vical, Inc. | Intracellular delivery of biologically active substances by means of self-assembling lipid complexes |
| US5498830A (en) | 1990-06-18 | 1996-03-12 | Monsanto Company | Decreased oil content in plant seeds |
| CA2051562C (en) | 1990-10-12 | 2003-12-02 | Jewel M. Payne | Bacillus thuringiensis isolates active against dipteran pests |
| US5173414A (en) | 1990-10-30 | 1992-12-22 | Applied Immune Sciences, Inc. | Production of recombinant adeno-associated virus vectors |
| US5399680A (en) | 1991-05-22 | 1995-03-21 | The Salk Institute For Biological Studies | Rice chitinase promoter |
| US5604121A (en) | 1991-08-27 | 1997-02-18 | Agricultural Genetics Company Limited | Proteins with insecticidal properties against homopteran insects and their use in plant protection |
| TW261517B (https=) | 1991-11-29 | 1995-11-01 | Mitsubishi Shozi Kk | |
| US5587308A (en) | 1992-06-02 | 1996-12-24 | The United States Of America As Represented By The Department Of Health & Human Services | Modified adeno-associated virus vector capable of expression from a novel promoter |
| US5789156A (en) | 1993-06-14 | 1998-08-04 | Basf Ag | Tetracycline-regulated transcriptional inhibitors |
| US5814618A (en) | 1993-06-14 | 1998-09-29 | Basf Aktiengesellschaft | Methods for regulating gene expression |
| US5837458A (en) | 1994-02-17 | 1998-11-17 | Maxygen, Inc. | Methods and compositions for cellular and metabolic engineering |
| US5605793A (en) | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
| US5608144A (en) | 1994-08-12 | 1997-03-04 | Dna Plant Technology Corp. | Plant group 2 promoters and uses thereof |
| US5659026A (en) | 1995-03-24 | 1997-08-19 | Pioneer Hi-Bred International | ALS3 promoter |
| US6072050A (en) | 1996-06-11 | 2000-06-06 | Pioneer Hi-Bred International, Inc. | Synthetic promoters |
| AR014072A1 (es) | 1998-02-26 | 2001-01-31 | Pioneer Hi Bred Int | Molecula de acido nucleico aislada que tiene una secuencia nucleotidica para un promotor que es capaz de iniciar una transcripcion constitutiva en unacelula de planta, construccion adn, vector, celula huesped, metodo para expresar en forma constitutiva una secuencia nucleotidica heteroloca en una |
| US6534261B1 (en) | 1999-01-12 | 2003-03-18 | Sangamo Biosciences, Inc. | Regulation of endogenous gene expression in cells using zinc finger proteins |
| US6555655B1 (en) | 1999-05-04 | 2003-04-29 | Monsanto Technology, Llc | Coleopteran-toxic polypeptide compositions and insect-resistant transgenic plants |
| US6593293B1 (en) | 1999-09-15 | 2003-07-15 | Monsanto Technology, Llc | Lepidopteran-active Bacillus thuringiensis δ-endotoxin compositions and methods of use |
| US20050183161A1 (en) | 2003-10-14 | 2005-08-18 | Athenix Corporation | AXMI-010, a delta-endotoxin gene and methods for its use |
| WO2005066202A2 (en) | 2003-12-22 | 2005-07-21 | E.I. Du Pont De Nemours And Company | Bacillus cry9 family members |
| AU2007260716B2 (en) | 2006-06-14 | 2013-05-02 | BASF Agricultural Solutions Seed US LLC | Axmi-031, axmi-039, axmi-040 and axmi-049, a family of delta-endotoxin genes and methods for their use |
| ES2609332T3 (es) | 2009-07-02 | 2017-04-19 | Athenix Corporation | Gen pesticida AXMI-205 y métodos para su uso |
| WO2011084324A2 (en) | 2009-12-21 | 2011-07-14 | Pioneer Hi-Bred International, Inc. | Novel bacillus thuringiensis gene with lepidopteran activity |
| WO2012024200A2 (en) | 2010-08-19 | 2012-02-23 | Pioneer Hi-Bred International, Inc. | Novel bacillus thuringiensis gene with lepidopteran activity |
| US9405700B2 (en) | 2010-11-04 | 2016-08-02 | Sonics, Inc. | Methods and apparatus for virtualization in an integrated circuit |
| AU2013266968B2 (en) | 2012-05-25 | 2017-06-29 | Emmanuelle CHARPENTIER | Methods and compositions for RNA-directed target DNA modification and for RNA-directed modulation of transcription |
| US8697359B1 (en) | 2012-12-12 | 2014-04-15 | The Broad Institute, Inc. | CRISPR-Cas systems and methods for altering expression of gene products |
| EP3186375A4 (en) | 2014-08-28 | 2019-03-13 | North Carolina State University | Novel CAS9 PROTEINS AND CHARACTERISTICS FOR DNA TARGETING AND GENOME EDITING |
| KR20230156800A (ko) | 2015-03-03 | 2023-11-14 | 더 제너럴 하스피탈 코포레이션 | 변경된 PAM 특이성을 갖는 조작된 CRISPR-Cas9 뉴클레아제 |
| US9790490B2 (en) | 2015-06-18 | 2017-10-17 | The Broad Institute Inc. | CRISPR enzymes and systems |
| PL3802807T3 (pl) | 2018-06-05 | 2025-03-31 | Life Edit Therapeutics, Inc. | Nukleazy kierowane rna i ich aktywne fragmenty oraz warianty i sposoby ich zastosowania |
| CA3125175A1 (en) * | 2018-12-27 | 2020-07-02 | LifeEDIT Therapeutics, Inc. | Polypeptides useful for gene editing and methods of use |
| KR20210149686A (ko) * | 2018-12-27 | 2021-12-09 | 라이프에디트 테라퓨틱스, 인크. | 유전자 편집에 유용한 폴리펩티드 및 사용 방법 |
| WO2020181195A1 (en) | 2019-03-06 | 2020-09-10 | The Broad Institute, Inc. | T:a to a:t base editing through adenine excision |
| WO2021030344A1 (en) | 2019-08-12 | 2021-02-18 | Lifeedit, Inc. | Rna-guided nucleases and active fragments and variants thereof and methods of use |
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