KR20230068736A - Bacillus velezensis JC-9 strain with plant growth promoting ability and plant disease control ability and use thereof - Google Patents
Bacillus velezensis JC-9 strain with plant growth promoting ability and plant disease control ability and use thereof Download PDFInfo
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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Abstract
Description
본 발명은 바실러스 벨레젠시스 (Bacillus velezensis) JC-9 (KACC81190BP) 및 이의 용도에 관한 것으로, 본 발명의 균주는 식물병 방제 효능 및 식물의 성장 촉진능이 우수하다.The present invention relates to Bacillus velezensis JC-9 (KACC81190BP) and its use, and the strain of the present invention has excellent plant disease control efficacy and plant growth promoting ability.
화학적 기술에 기초한 농약 및 비료는 자연생태계의 파괴, 병해충의 농약 저항성 증대, 토양과 수질 오염 야기 등 사회적 문제를 일으켜 왔다. 이에 최근 전 세계적으로 화학농약과 비료 사용을 줄이는 정책 시행으로 생물 농약, 생물 비료 시장규묘가 점점 커지고 있는 추세이고, 미생물을 이용한 친환경자재 시장이 점차적으로 증가하고 있다.Pesticides and fertilizers based on chemical technology have caused social problems such as destruction of the natural ecosystem, increased pesticide resistance of pests, and soil and water pollution. In recent years, with the implementation of policies to reduce the use of chemical pesticides and fertilizers around the world, the market size of biological pesticides and biofertilizers is gradually increasing, and the market for eco-friendly materials using microorganisms is gradually increasing.
그럼에도 국내 스마트 온실 농가 대상으로 실시한 조사(박영균, 2020)에 따르면 97.1%가 화학적 방법을 이용해 병해충을 방제하고 있다고 답변하였으며, 가장 큰 문제가 되는 식물병으로 탄저병과 같은 곰팡이성 병해로 응답하였다고 한다.Nevertheless, according to a survey conducted on domestic smart greenhouse farmhouses (Park Young-gyun, 2020), 97.1% answered that they were using chemical methods to control pests, and responded with fungal diseases such as anthracnose as the biggest problem plant disease.
이처럼 친환경 기술에 대한 주목도가 증가하는 상황에도 실제 현장에서는 대부분 화학적 제제를 사용하는데, 아직 미생물을 활용한 친환경 제제가 효과가 느리고, 효과가 우수하지 못해서이다.In spite of the increasing attention on eco-friendly technology, most of the actual sites use chemical preparations, because the eco-friendly preparations using microorganisms are still slow and do not have excellent effects.
따라서, 이와 같은 문제점을 해결하기 위해 우량 균주 선별, 엄격한 품질 관리, 효능과 효과 검증, 미생물 활력 유지 및 실증 검증 등을 통한 맞춤형 사용 매뉴얼 구축 등의 기술력 연구가 필요한 상황이다.Therefore, in order to solve these problems, it is necessary to research technology such as selection of excellent strains, strict quality control, verification of efficacy and effectiveness, maintenance of microbial vitality, and establishment of customized use manuals through empirical verification.
본 발명의 목적은 식물병 유발 균주인 산토모나스 악소노포디스(Xanthomonas axonopodis)에 대한 항균능이 있으면서도, 식물 성장을 촉진시킬 수 있는 바실러스 벨레젠시스 (Bacillus velezensis) JC-9 (KACC81190BP) 및 이를 활용한 용도를 제공하고자 한다.An object of the present invention is Bacillus velezensis JC-9 (KACC81190BP), which can promote plant growth while having antibacterial activity against Xanthomonas axonopodis, which is a plant disease-causing strain, and utilization thereof. We want to serve a purpose.
본 발명은 식물병에 대해 방제효과 및 작물 생장 촉진 효과를 갖는 것을 특징으로 하는 바실러스 벨레젠시스 (Bacillus velezensis) JC-9 (KACC81190BP)를 제공한다.The present invention provides Bacillus velezensis JC-9 (KACC81190BP) characterized by having a control effect and crop growth promoting effect on plant diseases.
본 발명은 바실러스 벨레젠시스 (Bacillus velezensis) JC-9 (KACC81190BP), 이의 배양액 또는 이 배양액의 건조분말을 포함하는 것을 특징으로 하는 비료 조성물을 제공한다.The present invention provides a fertilizer composition comprising Bacillus velezensis JC-9 (KACC81190BP), its culture medium or dry powder of this culture medium.
본 발명은 바실러스 벨레젠시스 (Bacillus velezensis) JC-9 (KACC81190BP), 이의 배양액 또는 이 배양액의 건조분말을 포함하는 것을 특징으로 하는 농약 조성물을 제공한다.The present invention provides an agrochemical composition comprising Bacillus velezensis JC-9 (KACC81190BP), a culture medium thereof, or a dry powder of the culture medium.
본 발명의 바실러스 벨레젠시스 (Bacillus velezensis) JC-9 (KACC81190BP)는 식물병 유발 균주에 대하여 항균능이 있어 농약 조성물로 활용하기 좋고, 식물 성장을 촉진능이 좋아 비료 조성물로도 활용 가능하다. Bacillus velezensis JC-9 (KACC81190BP) of the present invention has antibacterial activity against plant disease-causing strains, so it can be used as an agrochemical composition, and can be used as a fertilizer composition because of its good ability to promote plant growth.
도 1은 본 발명 균주와 검은점 무늬병 세균인 Xanthomonas campestris pv. campestris KACC 17966를 배양하여 항균성을 확인한 실험 결과이다.
도 2는 본 발명 균주의 배양액에 식물 성장 조절 호르몬인 옥신(IAA)이 있는지 확인하기 위해 LC-MSMS 분석을 실시한 결과이다.
도 3 및 도 4는 본 발명 균주의 배양액에 항균 물질(바실로마이신 D (Bacillomycin D), 펜기신(Fengycin), 수르팩틴(Surfactin))이 있는지 확인하기 위해 LC-MSMS 분석을 실시한 결과이다.
도 5는 본 발명 균주의 사이드로포어(Siderophore) 생산량을 보여준다.
도 6은 본 발명 균주 배양액을 실제 작물에 미생물 비료로 사용하여 얻은 결과이다.1 shows the strain of the present invention and the black spot bacterium , Xanthomonas campestris pv. This is the experimental result of confirming the antibacterial activity by culturing campestris KACC 17966.
Figure 2 is a result of LC-MSMS analysis to confirm whether the plant growth regulating hormone auxin (IAA) is present in the culture medium of the strain of the present invention.
3 and 4 are the results of LC-MSMS analysis to determine whether antibacterial substances (Bacillomycin D, Fengycin, Surfactin) are present in the culture medium of the strain of the present invention.
Figure 5 shows the Siderophore production of the strain of the present invention.
6 is a result obtained by using the strain culture solution of the present invention as a microbial fertilizer for actual crops.
본 발명은 식물병에 대해 방제효과 및 작물 생장 촉진 효과를 갖는 것을 특징으로 하는 바실러스 벨레젠시스 (Bacillus velezensis) JC-9 (KACC81190BP)를 제공한다.The present invention provides Bacillus velezensis JC-9 (KACC81190BP) characterized by having a control effect and crop growth promoting effect on plant diseases.
하기 실시예에 따르면, 본 발명 바실러스 벨레젠시스 (Bacillus velezensis) JC-9 (KACC81190BP)는 식물병(검은점 무늬병) 유발 균주에 대해 방제효과를 가지고 있다. 또한, 본 발명 바실러스 벨레젠시스 (Bacillus velezensis) JC-9 (KACC81190BP)는 식물의 성장을 조절하는 호르몬인 옥신(IAA)를 생산하는데, 이에 본 발명 균주 배양액을 실제 작물에 처리한 결과 식물 성장 촉진능이 우수한 것을 확인할 수 있었다.According to the following examples, the Bacillus velezensis JC-9 (KACC81190BP) of the present invention has a control effect against strains causing plant disease (black spot). In addition, Bacillus velezensis JC-9 (KACC81190BP) of the present invention produces auxin (IAA), a hormone that regulates plant growth, and thus promotes plant growth as a result of treating the strain culture solution of the present invention in actual crops. It was confirmed that the performance was excellent.
본 발명은 바실러스 벨레젠시스 (Bacillus velezensis) JC-9 (KACC81190BP), 이의 배양액 또는 이 배양액의 건조분말을 포함하는 것을 특징으로 하는 비료 조성물 또는 농약 조성물을 제공한다.The present invention provides a fertilizer composition or an agricultural chemical composition comprising Bacillus velezensis JC-9 (KACC81190BP), its culture medium or dry powder of this culture medium.
한편, 상기 농약 조성물은 바람직하게 바실로마이신 D (Bacillomycin D), 펜기신(Fengycin), 수르팩틴(Surfactin), 사이드로포어(Siderophore)로 저해할 수 있는 식물병 방제 농약으로 사용하는 것이 좋다.On the other hand, the agrochemical composition is preferably used as a pesticide for controlling plant diseases that can be inhibited by Bacillomycin D, Fengycin, Surfactin, and Siderophore.
하기 실시예에 따르면, 본 발명 균주는 항균 물질인 바실로마이신 D (Bacillomycin D), 펜기신(Fengycin), 수르팩틴(Surfactin), 사이드로포어(Siderophore)를 생산한다.According to the following examples, the strain of the present invention produces antibacterial substances such as Bacillomycin D, Fengycin, Surfactin, and Siderophore.
이하, 본 발명의 내용을 하기 실시예를 통하여 보다 상세하게 설명하고자 한다. 다만, 본 발명의 권리범위가 하기 실시예에만 한정되는 것은 아니고 그와 등가의 기술적 사상의 변형까지를 포함한다.Hereinafter, the contents of the present invention will be described in more detail through the following examples. However, the scope of the present invention is not limited only to the following examples, and includes modifications of equivalent technical ideas.
[실시예 1: 후보 균주 확보 및 항균능 확인 실험][Example 1: Securing candidate strains and confirming antibacterial activity]
후보 균주들을 확보하기 위해, 국내 과수농장 밭 토양 1g을 채취하여 인산 완충 생리식염수 (Phosphate buffered saline, Ph 7.0)에 희석한 후, TSA(Tryptic Soy Agar)플레이트에 도말하여, 30 ℃에서 3일 이상 배양하였다. 이를 통해 50점의 균주를 분리할 수 있었다.To secure candidate strains, 1 g of soil from domestic orchard farms was collected, diluted in Phosphate buffered saline (Ph 7.0), and then spread on TSA (Tryptic Soy Agar) plates for 3 days or more at 30 ° C. cultured. Through this, it was possible to separate 50 strains.
이후 분리한 후보 균주를 검은점 무늬병 세균인 Xanthomonas campestris pv. campestris KACC 17966과 배양하여 항균능이 있는지 확인하고자 했다.Then, the isolated candidate strain was tested against the black spot bacterium, Xanthomonas campestris pv. It was cultured with campestris KACC 17966 to confirm its antibacterial activity.
항균능 확인 실험방법은 PDA배지 가운데에 후보 균주를 접종하고, 주변부에 검은점 무늬병 세균인 Xanthomonas campestris pv. campestris KACC 17966을 접종하였다. 이후 1주일간 30℃에서 정치배양하고, 상태를 관찰하였는데, 후보 균주 중 JC-9 균주가 우수한 항균능을 가지고 있었다 (도 1).The test method for confirming the antibacterial activity is to inoculate the candidate strain in the center of the PDA medium, and in the periphery, the black spot bacterium, Xanthomonas campestris pv. campestris KACC 17966 was inoculated. Thereafter, it was cultured at 30 ° C. for one week, and the condition was observed. Among the candidate strains, the JC-9 strain had excellent antibacterial activity (FIG. 1).
도 1은 본 발명 균주와 검은점 무늬병 세균인 Xanthomonas campestris pv. campestris KACC 17966를 배양하여 항균성을 확인한 실험 결과로, 검은점 무늬병 세균인 Xanthomonas campestris pv. campestris KACC 17966의 형태가 JC-9가 접종된 부분을 중심으로 동그랗게 깍여있는 모습을 볼 수 있는데, 이는 본 발명 균주 JC-9가 검은점 무늬병 세균인 Xanthomonas campestris pv. campestris KACC 17966에 대하여 우수한 항균능이 있음을 의미한다.1 shows the strain of the present invention and the black spot bacterium , Xanthomonas campestris pv. campestris KACC 17966 was cultured to confirm its antibacterial activity. As a result, the black spot bacterium Xanthomonas campestris pv. campestris KACC 17966 can be seen roundly cut around the inoculated part of JC-9, which indicates that the strain JC-9 of the present invention is a black spot bacterium, Xanthomonas campestris pv. campestris KACC 17966 means that there is an excellent antibacterial activity.
이하 실험에서는 본 발명 균주인 JC-9로만 실험하였다.In the following experiments, only the strain of the present invention, JC-9, was tested.
[실시예 2: 본 발명 균주의 식물성 호르몬 생산 확인 실험][Example 2: Plant Hormone Production Verification Test of the Inventive Strain]
본 발명 균주(JC-9)를 미생물 비료로 사용하였을 때 식물 생장 촉진 효과가 있을지 확인하기 위해, 본 발명 균주의 배양액에 식물호르몬 옥신(IAA)이 있는지 검출하고자 했다.In order to confirm whether the strain of the present invention (JC-9) has a plant growth promoting effect when used as a microbial fertilizer, it was attempted to detect the presence of the plant hormone auxin (IAA) in the culture medium of the strain of the present invention.
식물호르몬 옥신은 트립토판으로부터 합성되는 아미노산 유도체로서 식물의 성장을 조절하는 호르몬 중 하나로, 옥신은 식물 줄기와 잎, 뿌리의 성장을 촉진하고, 낙과를 방지하며, 착과를 촉진하는 것으로 알려져 있다.The plant hormone auxin is an amino acid derivative synthesized from tryptophan and is one of the hormones regulating the growth of plants.
한편, 옥신(IAA) 검출 실험방법은 JC-9 균주 배양액을 원심분리하여 얻은 상등액을 QuEChERS 방법을 사용하여 전처리하고, LC-MSMS 분석을 하였다 (도 2). 한편, LC-MSMS 분석은 컬럼으로 X-Bridge (2.1 x 100mm, 3.5㎛, C18)을 사용하고, flow rate를 0.2ml / min 으로 설정하여 실험하였다.On the other hand, in the auxin (IAA) detection experiment, the supernatant obtained by centrifuging the JC-9 strain culture medium was pretreated using the QuEChERS method, and LC-MSMS analysis was performed (FIG. 2). On the other hand, LC-MSMS analysis was performed by using an X-Bridge (2.1 x 100mm, 3.5㎛, C 18 ) as a column and setting the flow rate to 0.2ml / min.
도 2는 LC-MSMS 분석 결과를 보여주는데, 이를 보면 JC-9 균주가 식물 생장 촉진 물질인 옥신(IAA)을 생산할 수 있음을 확인할 수 있다. Figure 2 shows the results of LC-MSMS analysis, which confirms that the JC-9 strain can produce auxin (IAA), a plant growth promoter.
[실시예 3: 본 발명 균주의 항균 물질 생산 확인 실험][Example 3: Test for confirming antibacterial substance production of strains of the present invention]
본 발명 균주(JC-9)를 미생물 농약으로 사용하였을 때 어떤 항균 물질로 항균 효과를 나타내는지 확인하고자 했다.When the strain of the present invention (JC-9) was used as a microbial pesticide, it was intended to confirm which antibacterial substance exhibits an antibacterial effect.
실험방법은 JC-9 균주 배양액을 원심분리하여 얻은 상등액을 SPE(Solid-Phase Extraction) 방법을 사용하여 전처리하고, LC-MSMS 분석을 하였다 (도 3, 도 4). 한편, LC-MSMS 분석은 컬럼으로 X-Bridge (2.1 x 100mm, 3.5㎛, C18)을 사용하였고, flow rate를 0.2ml / min 으로 설정하였다.In the experimental method, the supernatant obtained by centrifuging the culture medium of the JC-9 strain was pretreated using the SPE (Solid-Phase Extraction) method, and LC-MSMS analysis was performed (FIGS. 3 and 4). Meanwhile, for LC-MSMS analysis, an X-Bridge (2.1 x 100 mm, 3.5 μm, C 18 ) was used as a column, and the flow rate was set to 0.2 ml / min.
도 3 및 도 4는 LC-MSMS 분석 결과를 보여주는데, 이를 보면 본 발명 균주의 배양액에는 항균 물질인 바실로마이신 D (Bacillomycin D), 펜기신(Fengycin), 수르팩틴(Surfactin)이 있는 것을 확인할 수 있다. 3 and 4 show the results of LC-MSMS analysis, which shows that the culture solution of the strain of the present invention has antibacterial substances such as Bacillomycin D, Fengycin, and Surfactin. there is.
한편, 해당 Mass peak가 바실로마이신 D (Bacillomycin D), 펜기신(Fengycin), 수르팩틴(Surfactin)을 나타내는게 맞는지는 'H.Chen 외 5인“Isolation and characterization of lipopeptide antibiotics produced byBacillus subtilis”, 2007, Letters in Applied Microbiology Volume 47, Issue 3 p. 180-186' 을 참고하여 확인할 수 있었다.On the other hand, whether the corresponding mass peak represents Bacillomycin D, Fengycin, and Surfactin is correct, 'H.Chen and 5 others “Isolation and characterization of lipopeptide antibiotics produced by Bacillus subtilis” , 2007, Letters in Applied Microbiology Volume 47,
[실시예 4: 본 발명 균주의 항균 물질 생산 확인 실험 2][Example 4:
본 발명 균주(JC-9)를 미생물 농약으로 사용하였을 때 어떤 항균 물질을 얼마나 생산하여 항균 효과를 나타내는지를 확인하고자 했다.When the strain of the present invention (JC-9) was used as a microbial pesticide, it was intended to confirm which antibacterial substance was produced and how much to show the antibacterial effect.
사이드로포어(Siderophore)는 토양 내 미량 존재하는 필수 생리물질인 철 성분을 선택적으로 흡수하여 병원균을 억제하는 물질이다.Siderophore is a substance that suppresses pathogens by selectively absorbing iron, an essential physiological substance present in trace amounts in the soil.
본 발명 균주의 사이드로포어 생산량은 CellAct-BS 배지에서 배양한 미생물 배양액 상등액을 ethyl acetate로 추출하여 Hathway's reagent와 반응 후 분광광도계로 700nm에서 흡광도를 측정하여 정량할 수 있었다 (도 5). 도 5을 보면, 본 발명 균주가 96시간에 8.52 mmol/ml 농도의 사이드로포어를 생산한 것을 확인할 수 있다. The siderophore production of the strain of the present invention was quantified by extracting the microbial culture supernatant cultured in CellAct-BS medium with ethyl acetate, reacting with Hathway's reagent, and measuring the absorbance at 700 nm with a spectrophotometer (FIG. 5). Referring to Figure 5, it can be seen that the strain of the present invention produced a siderophore at a concentration of 8.52 mmol / ml in 96 hours.
[실시예 5: 본 발명 균주의 식물 생장 촉진능 확인 실험][Example 5: Plant growth promoting ability test of the strain of the present invention]
본 발명 균주(JC-9) 배양액을 실제 작물에 미생물 비료로 사용하여도 효과가 있을지 확인하고자 했다.It was intended to confirm whether the culture solution of the present invention strain (JC-9) would be effective even if it was used as a microbial fertilizer for actual crops.
상추(청치마)에 배양액(1 x 10^9 CFU/ml)의 250배 희석액 또는 500배 희석액을 7일 간격으로 처리(엽면시비, 난괴법 방법 사용)하고, 변화를 관찰하였다 (도 6). 한편, 대조군은 배양액 무처리군으로 설정하였다.Lettuce (cheongchima) was treated with a 250-fold dilution or a 500-fold dilution of the culture medium (1 x 10^9 CFU/ml) every 7 days (foliar fertilization, using egg mass method), and changes were observed (FIG. 6). On the other hand, the control group was set as a culture medium untreated group.
상기 실험을 3회 반복하고, 생육정도를 구체적으로 알아보기 위해 상추의 엽수 및 엽생체중을 측정하고, 상추의 가장 긴 엽의 엽장, 엽폭, 낱중, 엽록소 함량을 측정하였다. 한편, 상기 엽록소 함량은 chlorophyll meter(model SPAD-50, Minolta, Japan.)로 가장 긴 엽의 중앙부위를 측정하였다.The above experiment was repeated three times, and the number of leaves and leaf weight of lettuce were measured to determine the degree of growth in detail, and the leaf length, leaf width, weight, and chlorophyll content of the longest leaf of lettuce were measured. Meanwhile, the chlorophyll content was measured in the center of the longest leaf with a chlorophyll meter (model SPAD-50, Minolta, Japan.).
상기 측정한 값의 평균값을 하기 표 1에 나타내었다. 각 조건별 측정 결과는 SPSS statistics 19 program을 이용하여 평균과 표준편차를 구하고 일원배치분산분석(one way ANOVA)을 실시한 후, Duncan’s multiple range test를 실시하여 유의성 검정하였다.The average value of the measured values is shown in Table 1 below. For the measurement results for each condition, the mean and standard deviation were obtained using the SPSS statistics 19 program, one way ANOVA was performed, and Duncan's multiple range test was performed to test significance.
(No./plant)ground game
(No./plant)
(SPAD)chlorophyll
(SPAD)
상기 표 1을 보면, 본 발명 균주가 처리된 (500배 희석액 처리구, 250배 희석액 처리구) 처리구의 경우 생장이 상당하게 촉진된 것을 확인할 수 있다.Referring to Table 1, it can be seen that the growth of the treatment groups treated with the strain of the present invention (500-fold dilution treatment group, 250-fold dilution treatment group) was significantly promoted.
[실시예 6: 본 발명 균주의 유전적 동정][Example 6: Genetic identification of strains of the present invention]
본 발명 균주(JC-9)를 16S rRNA 염기서열 분석 하여 동정을 수행하고자 했다. 분리 균주는 순수분리한 후 TSA 플레이트(Plate)상태로 냉장 택배를 이용하여 ㈜솔젠트(대전)에 의뢰하였고, 27F 및 1492R의 유니버설 프라이머 세트(universal primer set)를 사용하여 16S rRNA 염기서열 분석 수행하였다.Identification was attempted by analyzing the 16S rRNA sequencing of the strain of the present invention (JC-9). After pure isolation, the isolated strain was requested to Solgent Co., Ltd. (Daejeon) by refrigerated courier in a TSA plate state, and 16S rRNA sequencing was performed using 27F and 1492R universal primer sets. did
수행결과 본 발명 균주 JC-9의 16S rRNA 염기서열은 총 1,416bp 서열(서열번호 1)을 가진 것을 확인할 수 있었다.As a result, it was confirmed that the 16S rRNA nucleotide sequence of the strain JC-9 of the present invention had a total of 1,416 bp sequence (SEQ ID NO: 1).
해당 염기서열을 기초로 ncbi blast를 실행하였고, 그 결과 JC-9는 바실러스 벨레젠시스 (Bacillus velezensis)와 99%의 상동성을 나타냄을 확인할 수 있었고, 이에 본 발명 균주를 바실러스 벨레젠시스 (Bacillus velezensis) JC-9로 명명하였다.Based on the nucleotide sequence, ncbi blast was performed, and as a result , it was confirmed that JC-9 exhibits 99% homology with Bacillus velezensis. velezensis ) and named JC-9.
그 후, 본 발명 균주 바실러스 벨레젠시스 (Bacillus velezensis) JC-9를 농촌진흥청 국립농업과학원(KACC)에 10월 22일자로 기탁하여 수탁번호 KACC 81190BP를 부여받았다.Then, the strain of the present invention Bacillus velezensis ( Bacillus velezensis ) JC-9 was deposited with the National Academy of Agricultural Sciences (KACC) of the Rural Development Administration on October 22, and was given accession number KACC 81190BP.
기탁기관명 : 농촌진흥청 국립농업과학원Name of depository institution: Rural Development Administration National Institute of Agricultural Sciences
수탁번호 : KACCKACC81190BPAccession number: KACCKACC81190BP
수탁일자 : 20211022Entrusted date: 20211022
<110> Ecobiznet Inc. <120> Bacillus velezensis JC-9 strain with plant growth promoting ability and plant disease control ability and use thereof <130> YP-21-224 <160> 1 <170> KoPatentIn 3.0 <210> 1 <211> 1416 <212> DNA <213> Unknown <220> <223> Bacillus velezensis JC-9 <400> 1 gcagtcgagc ggacagatgg gagcttgctc cctgatgtta gcggcggacg ggtgagtaac 60 acgtgggtaa cctgcctgta agactgggat aactccggga aaccggggct aataccggat 120 ggttgtttga accgcatggt tcagacataa aaggtggctt cggctaccac ttacagatgg 180 acccgcggcg cattagctag ttggtgaggt aacggctcac caaggcgacg atgcgtagcc 240 gacctgagag ggtgatcggc cacactggga ctgagacacg gcccagactc ctacgggagg 300 cagcagtagg gaatcttccg caatggacga aagtctgacg gagcaacgcc gcgtgagtga 360 tgaaggtttt cggatcgtaa agctctgttg ttagggaaga acaagtgccg ttcaaatagg 420 gcggcacctt gacggtacct aaccagaaag ccacggctaa ctacgtgcca gcagccgcgg 480 taatacgtag gtggcaagcg ttgtccggaa ttattgggcg taaagggctc gcaggcggtt 540 tcttaagtct gatgtgaaag cccccggctc aaccggggag ggtcattgga aactggggaa 600 cttgagtgca gaagaggaga gtggaattcc acgtgtagcg gtgaaatgcg tagagatgtg 660 gaggaacacc agtggcgaag gcgactctct ggtctgtaac tgacgctgag gagcgaaagc 720 gtggggagcg aacaggatta gataccctgg tagtccacgc cgtaaacgat gagtgctaag 780 tgttaggggg tttccgcccc ttagtgctgc agctaacgca ttaagcactc cgcctgggga 840 gtacggtcgc aagactgaaa ctcaaaggaa ttgacggggg cccgcacaag cggtggagca 900 tgtggtttaa ttcgaagcaa cgcgaagaac cttaccaggt cttgacatcc tctgacaatc 960 ctagagatag gacgtcccct tcgggggcag agtgacaggt ggtgcatggt tgtcgtcagc 1020 tcgtgtcgtg agatgttggg ttaagtcccg caacgagcgc aacccttgat cttagttgcc 1080 agcattcagt tgggcactct aaggtgactg ccggtgacaa accggaggaa ggtggggatg 1140 acgtcaaatc atcatgcccc ttatgacctg ggctacacac gtgctacaat ggacagaaca 1200 aagggcagcg aaaccgcgag gttaagccaa tcccacaaat ctgttctcag ttcggatcgc 1260 agtctgcaac tcgactgcgt gaagctggaa tcgctagtaa tcgcggatca gcatgccgcg 1320 gtgaatacgt tcccgggcct tgtacacacc gcccgtcaca ccacgagagt ttgtaacacc 1380 cgaagtcggt gaggtaacct ttaggagcca gccgcc 1416 <110> Ecobiznet Inc. <120> Bacillus velezensis JC-9 strain with plant growth promoting ability and plant disease control ability and use thereof <130> YP-21-224 <160> 1 <170> KoPatentIn 3.0 <210> 1 <211> 1416 <212> DNA <213> unknown <220> <223> Bacillus velezensis JC-9 <400> 1 gcagtcgagc ggacagatgg gagcttgctc cctgatgtta gcggcggacg ggtgagtaac 60 acgtgggtaa cctgcctgta agactgggat aactccggga aaccggggct aataccggat 120 ggttgtttga accgcatggt tcagacataa aaggtggctt cggctaccac ttacagatgg 180 acccgcggcg cattagctag ttggtgaggt aacggctcac caaggcgacg atgcgtagcc 240 gacctgagag ggtgatcggc cacactggga ctgagacacg gcccagactc ctacgggagg 300 cagcagtagg gaatcttccg caatggacga aagtctgacg gagcaacgcc gcgtgagtga 360 tgaaggtttt cggatcgtaa agctctgttg ttagggaaga acaagtgccg ttcaaatagg 420 gcggcacctt gacggtacct aaccagaaag ccacggctaa ctacgtgcca gcagccgcgg 480 taatacgtag gtggcaagcg ttgtccggaa ttattgggcg taaagggctc gcaggcggtt 540 tcttaagtct gatgtgaaag cccccggctc aaccggggag ggtcattgga aactggggaa 600 cttgagtgca gaagaggaga gtggaattcc acggttagcg gtgaaatgcg tagagatgtg 660 gaggaacacc agtggcgaag gcgactctct ggtctgtaac tgacgctgag gagcgaaagc 720 gtggggagcg aacaggatta gataccctgg tagtccacgc cgtaaacgat gagtgctaag 780 tgttaggggg tttccgcccc ttagtgctgc agctaacgca ttaagcactc cgcctgggga 840 gtacggtcgc aagactgaaa ctcaaaggaa ttgacggggg cccgcacaag cggtggagca 900 tgtggtttaa ttcgaagcaa cgcgaagaac cttaccaggt cttgacatcc tctgacaatc 960 ctagagatag gacgtcccct tcgggggcag agtgacaggt ggtgcatggt tgtcgtcagc 1020 tcgtgtcgtg agatgttggg ttaagtcccg caacgagcgc aacccttgat cttagttgcc 1080 agcattcagt tgggcactct aaggtgactg ccggtgacaa accggaggaa ggtggggatg 1140 acgtcaaatc atcatgcccc ttatgacctg ggctacacac gtgctacaat ggacagaaca 1200 aagggcagcg aaaccgcgag gttaagccaa tcccacaaat ctgttctcag ttcggatcgc 1260 agtctgcaac tcgactgcgt gaagctggaa tcgctagtaa tcgcggatca gcatgccgcg 1320 gtgaatacgt tcccgggcct tgtacacacc gcccgtcaca ccacgagagt ttgtaacacc 1380 cgaagtcggt gaggtaacct ttaggagcca gccgcc 1416
Claims (3)
Bacillus velezensis JC-9 (KACC81190BP), characterized by having a control effect and crop growth promoting effect on plant diseases.
Bacillus velezensis ( Bacillus velezensis ) JC-9 (KACC81190BP), a fertilizer composition comprising a culture medium thereof or a dry powder of the culture medium.
Bacillus velezensis ( Bacillus velezensis ) JC-9 (KACC81190BP), a pesticide composition characterized in that it comprises a culture medium thereof or dry powder of the culture medium.
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Citations (5)
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|---|---|---|---|---|
| KR20140051698A (en) * | 2012-10-23 | 2014-05-02 | 한국화학연구원 | Microorganism formulation for controlling plant diseases containing bacillus velezensis g341 and lysinibacillus sphaericus tc1 strains and method for controlling plant diseases using the same |
| KR20140127670A (en) | 2013-04-25 | 2014-11-04 | (주) 제일그린산업 | Endophytic bacteria Bacillus Methylotrophicus YC7007 and development of a multifunctional biopesticide and microbial fertilizer using same |
| KR101535893B1 (en) | 2013-11-15 | 2015-07-14 | 대한민국 | New microorganism Bacillus amyloliquefaciens CC110 and Microbial agent biopesticide containing the same |
| JP2015534457A (en) * | 2012-08-14 | 2015-12-03 | マローネ バイオ イノベーションズ，インコーポレイテッド | Bacillus sp. Strain having antifungal, antibacterial and growth promoting activity |
| KR102078252B1 (en) * | 2018-12-18 | 2020-02-17 | 경기도 | Bacillus velezensis ARRI17 strain having antifungal activity against Root Rot Pathogen of ginseng and Use Thereof |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2015534457A (en) * | 2012-08-14 | 2015-12-03 | マローネ バイオ イノベーションズ，インコーポレイテッド | Bacillus sp. Strain having antifungal, antibacterial and growth promoting activity |
| KR20140051698A (en) * | 2012-10-23 | 2014-05-02 | 한국화학연구원 | Microorganism formulation for controlling plant diseases containing bacillus velezensis g341 and lysinibacillus sphaericus tc1 strains and method for controlling plant diseases using the same |
| KR20140127670A (en) | 2013-04-25 | 2014-11-04 | (주) 제일그린산업 | Endophytic bacteria Bacillus Methylotrophicus YC7007 and development of a multifunctional biopesticide and microbial fertilizer using same |
| KR101535893B1 (en) | 2013-11-15 | 2015-07-14 | 대한민국 | New microorganism Bacillus amyloliquefaciens CC110 and Microbial agent biopesticide containing the same |
| KR102078252B1 (en) * | 2018-12-18 | 2020-02-17 | 경기도 | Bacillus velezensis ARRI17 strain having antifungal activity against Root Rot Pathogen of ginseng and Use Thereof |
Non-Patent Citations (1)
| Title |
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| Front. Microbiol., Vol.10, Article No.2610, pp.1-25(2019.11.)* * |
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| KR102656678B1 (en) | 2024-04-12 |
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