KR20230047628A - Cosmetic composition for moisturizing skin containing fermentative betula alba juice as active ingredient - Google Patents

Abstract

The present invention relates to a cosmetic composition for moisturizing skin containing fermented Betula platyphylla sap as an active ingredient. The present invention was completed by proving, through experiments, that the cosmetic composition of the present invention is obtained through fermentation of the Betula platyphylla sap and has a superior skin protection effect compared to that of a cosmetic composition using the above ingredients alone. The fermented Betula platyphylla sap of the present invention maintains skin moisture homeostasis and causes little irritation to the skin, thereby being used as an active ingredient in cosmetic compositions.

Description

Moisturizing cosmetic composition containing fermented manjusa birch sap as an active ingredient

The present invention relates to a skin moisturizing cosmetic composition containing fermented Manchurian birch sap as an active ingredient.

The skin forms a major barrier from the external environment and plays an important role in protecting our body from wounds, infections, moisture loss, and ultraviolet rays. Ultraviolet rays, etc., are one of the main causes of skin aging by damaging the skin, and in the field of skin research, a lot of research is being conducted on the correlation between ultraviolet rays and the skin.

Skin moisturizing protects the body from various external stimuli and at the same time has a barrier function that blocks moisture from the inside. will do Structurally, the cells of the stratum corneum act as bricks, and the intercellular lipids of the keratinocytes act as mortar to form the skin barrier.

However, as people age, dry skin tends to increase, and the need for skin moisturizers is increasing due to indoor heating and air pollution. To this end, polyhydric alcohols and saccharides have been used as skin moisturizers in combination with cosmetics as general moisturizers. However, there is a problem that stickiness occurs on the skin, and there is also a problem that does not give a sufficient moisturizing effect to the skin. In addition, in the summer when the humidity is very high, the skin is sticky by drawing moisture from the outside, and in the winter, the humidity of the external air is low, rather pulling the moisture from the skin, causing the skin to become dry.

Human skin is very sensitive to relative humidity and usually feels comfortable when the relative humidity is between 40 and 70%. Relative humidity is a value expressed as a percentage of the ratio of the amount of water vapor contained in 1 m ³ of air to the amount of saturated water vapor at the same temperature. Human skin is also greatly affected by seasonal changes and changes in humidity. In summer, when the relative humidity is high due to high temperature and humidity, discomfort increases, and in the dry winter season, when the relative humidity is low, the lips and skin also become dry and cracked, sometimes causing difficulty in breathing. I can feel it. When the relative humidity is high, it absorbs moisture in the air and becomes sticky, and when the relative humidity is low, the moisture in the skin evaporates and becomes dry. In particular, when the relative humidity is low, the dryness of the skin is often accompanied by tightness and itching.

Various cosmetic compositions have tried to improve the dryness of the skin by using various moisturizers such as oil, sugar, and polyol to overcome the dryness of the skin. For example, by strengthening the skin barrier function using ceramide, cholesterol, fatty acids, etc., which are components of the skin, to suppress moisture evaporating from the skin, or to increase the ability of the skin to retain moisture using sugars and polyols that have a strong ability to combine with moisture on the skin surface. It was intended to enhance the moisturizing effect of the skin. However, it is difficult to maintain the effect for a long time in winter when the relative humidity is low, and it is rather unpleasant in summer when the relative humidity is high, making it difficult to manufacture an appropriate cosmetic composition according to the change in relative humidity.

Korean Patent Registration No. 10-2247966 discloses a cosmetic composition containing a mixed extract of mung bean seeds, Manchuria birch sap, and root of japonica that exhibits a skin moisturizing effect.

In addition, Korean Patent Publication No. 10-2017-0103172 discloses a cosmetic composition containing centella asiatica extract, ceramide and birch sap that exhibits a skin barrier improving effect.

Accordingly, the inventors of the present invention constantly maintained the moisturizing power of the skin regardless of seasonal changes and changes in relative humidity and continued research to manufacture a cosmetic composition that maintained the effect for a long time, and as a result, fermentation of Manju birch sap The present invention was completed by confirming that, when applied to the skin, a continuous moisturizing effect could be exhibited regardless of changes in relative humidity.

Accordingly, an object of the present invention is to provide a cosmetic composition for moisturizing the skin that provides continuous moisturizing power regardless of changes in relative humidity. Other objects and advantages of the present invention will become more apparent from the following detailed description and claims.

The above tasks are Saccharomyces cerevisiae J2K-413, Lactobacillus brevis J2K-367, Lactobacillus rhamnosus J2K-187, Bacillus subtilis It is achieved by a cosmetic composition for skin moisturizing containing fermented manjusa birch sap fermented with J2K-351 or a mixed strain thereof as an active ingredient.

Preferably, the fermented Manchurian birch sap is prepared from Saccharomyces cerevisiae J2K-413, Lactobacillus brevis J2K-367, Lactobacillus rhamnosus J2K -187 and Bacillus subtilis J2K-351 respectively.

Also preferably, the cosmetic composition has a skin irritation relieving effect and a skin protecting effect.

Also preferably, the cosmetic composition is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray. It may have a formulation selected from.

The present invention provides a cosmetic composition containing fermented Manchurian birch sap as an active ingredient. The fermented Manchuria birch sap of the present invention maintains skin moisturizing homeostasis and hardly irritates the skin, so it can be used as an active ingredient in a cosmetic composition.

In describing the present invention, if it is determined that a detailed description of a related known technology or configuration may unnecessarily obscure the subject matter of the present invention, the detailed description will be omitted. In addition, the terms to be described below are terms defined in consideration of functions in the present invention, which may vary according to the intention or custom of the user or operator, so the definitions should be made based on the content throughout this specification describing the present invention.

The present invention relates to a cosmetic composition for enhancing skin moisturization containing fermented Manchurian birch sap. The fermented Manchuria birch sap according to the present invention has skin irritation relief and skin protection effects, and can exhibit moisturizing improvement effects.

Hereinafter, the present invention will be described in more detail.

Mandarin birch (Betula Alba) used as an active ingredient in the present invention is a plant of the birch family, which grows in the deep forests of the northern region. Birch wood is very hard and dense, so it is used to make furniture and wood crafts. Manju birch sap refers to the liquid that goes up to the leaves through the canopy in the trunk of the tree, and is mainly collected in early spring.

The fermented Manchurian birch sap used in the present invention refers to a product obtained by fermenting collected Manchurian birch sap. Specifically, the fermented Manchuria birch sap means fermented Manchuria birch sap with one or more bacteria selected from the group consisting of yeast, lactic acid bacteria, acetic acid bacteria, bacillus bacteria, and aspergillus bacteria.

For example, the yeast may be selected from the group consisting of Saccharomyces cerevisiae, Schizosaccharomyces ellipsoideus, and Saccharomyces boulardii. . More preferably, the Saccharomyces cerevisiae J2K-413 strain isolated and identified from traditional fermentation strains can be used.

For example, the lactic acid bacteria are Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus bulgaricus, Lactobacillus rhamnosus, Lactobacillus plantarum plantarum), Lactobacillus reuteri, Lactobacillus confusus, Lactobacillus fermentum, Lactobacillus brevis, and Thermus thermophilus. One or more may be selected and used from. More preferably, Lactobacillus brevis J2K-367 isolated and identified from mistletoe flowers and Lactobacillus rhamnosus J2K-187 isolated and identified from mistletoe flowers can be used.

For example, Acetobacter can utilize microorganisms of the genus Acetobacter. Specifically, one or more species may be selected and used from the group consisting of Acetobacter xylinum, Acetobacter pasteurianus, and Acetobacter hansenii.

For example, the Bacillus strain is selected from the group consisting of Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium, and Bacillus mesentericus. and can be used More preferably, Bacillus subtilis J2K-351 isolated and identified from chintz can be used.

For example, the Aspergillus bacteria may be used by selecting one or more from the group consisting of Aspergillus usami and Aspergillus oryzae.

According to one embodiment of the present invention, the fermented Manchurian birch sap is produced from Saccharomyces cerevisiae J2K-413, Lactobacillus brevis J2K-367, Lactobacillus rhamnosus (Lactobacillus rhamnosus) J2K-187 and Bacillus subtilis (Bacillus subtilis) J2K-351 means a mixture in which fermented products are mixed in a weight ratio of 1:1:1:1.

As the fermentation broth according to the present invention, fermentation broth itself, dried fermentation broth, filtrate from fermentation broth, centrifuged fermentation broth, or purified products of these materials may be used, but is not limited thereto.

Preferably, the fermented Manchuria birch sap of the present invention can be prepared as follows.

a) preparation steps such as collecting Manchurian birch sap and filtering it through a filter;

b) fermenting the product of step a) using one or more bacteria selected from the group consisting of yeast, lactic acid bacteria, acetic acid bacteria, bacillus bacteria, and aspergillus bacteria to produce a fermented product.

More preferably, c) aging the fermented product of step b) at a low temperature may be further included.

Specifically, in step a), the collected manju birch sap is filtered through a filter cloth.

In step b), after high-pressure sterilization of the result of step a), one or more bacteria selected from yeast, lactic acid bacteria, acetic acid bacteria, bacillus bacteria, or aspergillus bacteria are inoculated. Then, it is preferably made in a fermentation tank at 20 to 40 ° C. and pH 4 to 7 for 7 to 28 days, and the fermentation product is obtained by vacuum sterilization, followed by centrifugation and filtration. The fermentation time may vary depending on the fermentation temperature, and the fermentation conditions may vary depending on the state of the sap, so the contents of the present invention are not limited to the fermentation temperature, pH of the culture medium and fermentation time exemplified above.

In step c), it is preferable to age the fermented product obtained in step b) at 5 to 20 ° C. and 75 to 85% humidity for 10 to 30 days. no.

The cosmetic composition of the present invention contains fermented manjusa birch sap capable of maintaining skin moisturizing homeostasis. The content of the fermented Manchurian birch sap may be 0.0001 to 70.0% by weight, preferably 0.001 to 50.0% by weight, based on the total weight of the cosmetic composition. If the content of the fermented Manchurian birch sap is less than 0.0001% by weight based on the total weight of the cosmetic composition, the skin moisturizing effect is somewhat insufficient, and the content of the fermented Manchurian birch sap is 70% by weight based on the total weight of the cosmetic composition. If it is exceeded, a problem may occur in the stability of the formulation according to the increase in the content. It is preferably used in an amount of 0.0001 to 70% by weight based on the total weight of the cosmetic composition of the present invention, but is not limited thereto.

Ingredients included in the cosmetic composition of the present invention may include ingredients commonly used in cosmetic compositions in addition to the above active ingredients as active ingredients, such as antioxidants, sunscreens, stabilizers, solubilizers, vitamins, pigments, and fragrances. and the same conventional adjuvants, and carriers.

The cosmetic composition of the present invention can be prepared in any formulation conventionally prepared in the art, for example, a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing , Oil, powder foundation, emulsion foundation, wax foundation, sunscreen emulsion, sunscreen cream, pack, sheet mask, massage cream and spray, etc., but may be formulated, but is not limited thereto. More specifically, it may be prepared in the form of softening lotion, nourishing lotion, nourishing cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.

The cosmetic composition containing the fermented Manchurian birch sap of the present invention is a cosmetic that helps maintain skin moisturizing homeostasis, and has excellent skin safety with low irritation to the skin. This fact is detailed in the preparation examples below.

Hereinafter, the present invention will be described in more detail through preparation examples. These preparation examples are only for explaining the present invention in more detail, and it will be apparent to those skilled in the art that the scope of the present invention is not limited by these preparation examples according to the gist of the present invention. .

Preparation Examples 1 to 4: Preparation of fermented Manju birch sap

Manchurian birch sap used in the present invention was purchased and used commercially available Manchurian birch sap. The fermenting strain was inoculated into 100 g of filtered Manchuria birch sap. The fermented strain, in Production Example 1, was isolated and identified from the traditional fermentation strain, and Saccharomyces cerevisiae J2K-413 belonging to the yeast was isolated and identified in Production Example 2, Lactobacillus brevis Lactobacillus brevis ) J2K-367, in Preparation Example 3, was isolated and identified from colostrum, Lactobacillus rhamnosus J2K-187, which was isolated and identified from colostrum, in Preparation Example 4, was isolated and identified from chintz, and Bacillus subtilis belonging to Bacillus subtilis) J2K-351 was used.

Then, it was fermented for 14 days at 30 ° C. and pH 5 in a fermenter. After fermentation, the fermented liquid was vacuum sterilized at 85 ° C., centrifuged to first remove the fermenting bacteria, and the fermented product filtered with Whatman # 4 filter paper was prepared by aging for 20 days at 15 ° C. and 80% humidity conditions.

Comparative Preparation Example 1: Manchuria birch sap

Comparative Preparation Example For Manju birch sap, commercially available Manju birch sap was purchased, filtered, and used.

Comparative Preparation Example 2: Manchuria Birch Bark Extract

Manchurian birch bark used in the present invention was purchased and used commercially sold Manchurian birch bark. 100 g of shade-dried Manchuria birch bark was extracted with reflux three times for 5 hours each with 70% (V/V) ethanol aqueous solution, cooled, and then filtered with Whatman #4 filter paper. The filtered extract was concentrated under reduced pressure and freeze-dried at 50° C. or less to obtain 13.1 g of Manchuria birch bark extract.

Comparative Preparation Example 3: Preparation of Fermented Manju Birch Sap

For the fermented Manchuria birch sap of Comparative Preparation Example 3, commercially available Manchurian birch sap was purchased and used. Lactobacillus acidophilus, a lactic acid bacterium isolated and identified from milk, was inoculated into 100 g of the filtered Manchuria birch sap as a fermenting strain. Then, it was fermented for 14 days at 30 ° C. and pH 5 in a fermenter. After fermentation, the fermented liquid was vacuum sterilized at 85 ° C., centrifuged to first remove the fermenting bacteria, and the fermented product filtered with Whatman # 4 filter paper was prepared by aging for 20 days at 15 ° C. and 80% humidity conditions.

Preparation of Examples and Comparative Examples

Examples 1 to 4 are formulations containing the fermented Manchurian birch sap prepared in Preparation Examples 1 to 4, respectively, and Example 5 is a formulation containing the fermented Manchurian birch sap prepared in Preparation Examples 1 to 4 in a 1:1:1: It was prepared as shown in Table 1 below as a formulation containing 1. Comparative Examples 1 and 2 each contained Manchurian birch sap and Manchurian birch bark extract, Comparative Example 3 did not contain the components related to the present invention, and Comparative Example 4 contained the Lactobacillus prepared in Comparative Preparation Example 3. It contains fermented Manchuria birch sap using Acidophilus.

Ingredients (unit: % by weight) Example 1 Example 2 Example 3 Example 4 Example 5 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Fermented Manchuria Birch Sap
/ J2K-413 (Production Example 1) 3.0 - - - 0.75 - - - - Fermented Manchuria Birch Sap
/ J2K-367 (Production Example 2) - 3.0 - - 0.75 - - - - Fermented Manchuria Birch Sap
/ J2K-187 (Production Example 3) - - 3.0 - 0.75 - - - - Fermented Manchuria Birch Sap
/ J2K-351 (Production Example 4) - - - 3.0 0.75 - - - - Manchurian birch sap
(Comparative Manufacturing Example 1) - - - - - 3.0 - - - Manju birch bark extract
(Comparative Manufacturing Example 2) - - - - - - 3.0 - - Fermented Manchuria Birch Sap
(Comparative Manufacturing Example 3) - - - - - - 3.0 - glycerin 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 EDTA-2Na 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 Purified water to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100 Cetostearyl Alcohol 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 Glyceryl Stearate 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 microcrystalline 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 squalane 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 trioctanoin 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 polysorbate 1.2 1.2 1.2 1.2 1.2 1.2 1.2 1.2 1.2 Sorbitan Stearate 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Tocopheryl Acetate 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 cyclomethicone 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 fragrance, preservative Appropriate amount Appropriate amount Appropriate amount Appropriate amount Appropriate amount Appropriate amount Appropriate amount Appropriate amount Appropriate amount

Experimental Example 1: Moisturizing effect and persistence

The clinical moisturizing effect and durability of the cosmetic compositions of Examples 1 to 5 were measured as follows. Through a questionnaire survey, 25 healthy adult men and women who felt their skin was dry were asked to apply the cosmetic compositions of Examples 1 to 5 and Comparative Examples 1 to 3 twice a day for 4 weeks on their upper arms. The moisturizing effect was evaluated by quantifying the change in electrical conductivity according to the amount of moisture in the epidermis using a Corneometer CM 820 (Corage + Khazaka, Germany) for 2 weeks from the start of the practical use test and after the end of the test. The experimental results are shown in Table 2 below.

division before use after 2 weeks of use after 4 weeks of use Example 1 22.6 38.9 45.9 Example 2 24.5 40.4 47.0 Example 3 21.9 39.4 45.8 Example 4 23.2 38.3 46.5 Example 5 22.4 43.5 48.9 Comparative Example 1 23.4 35.5 35.4 Comparative Example 2 24.1 33.2 32.3 Comparative Example 3 23.3 36.5 39.1 Comparative Example 4 24.5 30.6 32.0

As shown in Table 2, it was confirmed that the moisturizing effect of the cosmetic composition of the present invention (Examples 1 to 5) was very excellent compared to Comparative Examples 1 to 3.

Experimental Example 2: Skin barrier improvement effect

The study was conducted on 25 healthy women in a room at 25°C, 45% relative humidity, and no air flow. Skin barrier damage was induced using a 1% aqueous solution of sodium lauryl sulfate (SLS), and after applying the cosmetic compositions of Examples 1 to 5 and Comparative Examples 1 to 4 on the upper arm, TEWAMETER TM210 ( Transepidermal water loss was measured using C+K eletronic GmhH. Germany). That is, the skin barrier recovery rate was confirmed by measuring the amount of change in the TEWL value according to the change in TEWL (Transepidermal Water Loss) value over time. The experimental results are shown in Table 3 below.

division Skin barrier recovery rate (%) 2D 4D 6D 8D Example 1 48.8 66.6 81.9 97.7 Example 2 49.2 65.8 81.5 98.2 Example 3 50.1 68.0 83.1 99.4 Example 4 49.0 66.8 82.4 98.6 Example 5 53.7 71.1 88.6 99.0 Comparative Example 1 39.9 53.6 64.4 75.4 Comparative Example 2 40.1 51.7 63.5 76.1 Comparative Example 3 41.3 56.8 69.9 81.3 Comparative Example 4 36.1 49.3 59.8 71.3

As shown in Table 3, it was confirmed that Examples 1 to 5 containing the cosmetic composition of the present invention showed a relatively high skin barrier recovery rate compared to Comparative Examples 1 to 4.

Experimental Example 3: Skin irritation relief effect

The experiment was conducted simultaneously with the moisturizing effect 2-skin barrier improvement effect experiment of Experimental Example 2. The study was conducted on 25 healthy women in a room at 25°C, 45% relative humidity, and no air flow. Skin barrier damage and skin erythema were induced using a 1% aqueous solution of sodium lauryl sulfate (SLS), and after applying the cosmetic compositions of Examples 1 to 5 and Comparative Examples 1 to 4 on the upper arm, Skin redness (a value*) was measured using a color difference meter (Minolta chromameter CR300, Japan). That is, the rate of improvement in skin irritation erythema was confirmed by measuring the amount of change in skin redness value over time. The experimental results are shown in Table 4 below.

division Skin erythema improvement rate (%) 2D 4D 6D 8D Example 1 56.8 84.5 94.0 99.8 Example 2 56.2 85.8 93.2 100.2 Example 3 55.1 86.1 95.1 99.7 Example 4 57.1 86.0 92.3 99.9 Example 5 59.7 89.1 97.8 100.0 Comparative Example 1 50.0 62.5 84.5 90.3 Comparative Example 2 50.2 61.4 81.2 89.9 Comparative Example 3 51.6 65.7 88.0 93.1 Comparative Example 4 39.1 58.0 69.2 81.0

As shown in Table 4, it was confirmed that Examples 1 to 5 containing the cosmetic composition of the present invention showed a relatively high rate of improvement in erythema due to skin irritation compared to Comparative Examples 1 to 4.

Experimental Example 4: Skin protection effect against ultraviolet rays

In order to confirm the skin protection by ultraviolet rays of the cosmetics containing the fermented Manchuria birch sap of the present invention, the following experiment was conducted. The experiment was conducted on 30 healthy subjects, and skin erythema was induced by irradiating ultraviolet rays (UVB, 1.5MED) on the subject's back skin. Before irradiation with ultraviolet rays, Examples 1 to 5 and Comparative Examples 1 to 4 were pretreated and applied, and after irradiation with ultraviolet rays, Examples 1 to 5 and Comparative Examples 1 to 4 were applied three times at 2-hour intervals. The degree of erythema generation was visually evaluated after 48 hours after UV irradiation, and instrumental evaluation was also performed for objective evaluation. Instrument evaluation was measured using a color difference meter (Minolta chromameter CR300, Japan), and the results are shown in Table 5 below.

degree of erythema division Number of people with erythema (persons) evaluation (a value*) before UV irradiation 0 5.1 Example 1 2 5.8 Example 2 3 6.5 Example 3 2 6.0 Example 4 3 6.3 Example 5 3 6.4 Comparative Example 1 9 10.9 Comparative Example 2 10 10.5 Comparative Example 3 7 8.2 Comparative Example 4 12 12.1

Through the results of Table 5, it was confirmed that Examples 1 to 5 significantly reduced the degree of erythema production compared to Comparative Examples 1 to 4, and the erythema reduction effect was very excellent compared to Comparative Example 4 that did not contain the active ingredient. Confirmed.

Therefore, it can be seen that the cosmetic composition containing the fermented Manchurian birch sap of the present invention has a very excellent effect of inhibiting skin damage caused by ultraviolet rays, and thus is useful as a cosmetic composition for skin protection.

Having described specific parts of the present invention in detail above, it is clear that these specific techniques are only preferred embodiments for those skilled in the art, and the scope of the present invention is not limited thereto. Accordingly, the substantial scope of the present invention will be defined by the appended claims and equivalents thereof.

Claims (4)

Saccharomyces cerevisiae J2K-413, Lactobacillus brevis J2K-367, Lactobacillus rhamnosus J2K-187, Bacillus subtilis J2K-351 or these A cosmetic composition for skin moisturizing containing fermented Manju birch sap fermented with a mixed strain of as an active ingredient. According to claim 1,
The fermented Manchurian birch sap was prepared by Saccharomyces cerevisiae J2K-413, Lactobacillus brevis J2K-367, Lactobacillus rhamnosus J2K-187 and Bacillus A cosmetic composition for moisturizing skin, characterized in that it is a mixture of fermented products each fermented with Bacillus subtilis J2K-351. According to claim 1,
The cosmetic composition has a skin irritation relief effect and a skin protection effect, a cosmetic composition for moisturizing the skin. According to claim 1,
The cosmetic composition is a formulation selected from the group consisting of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations and sprays. Having, a cosmetic composition for moisturizing the skin.