KR102042594B1 - Cosmetic composition for moisturizing skin containing fermentative extract of brassica oleracea var. capitata - Google Patents

Abstract

The present invention relates to a moisturizing cosmetic composition containing a fermented cabbage extract. Specifically, the fermented cabbage extract is fermented with one or more bacteria selected from the group consisting of yeast, lactic acid bacteria, acetic acid bacteria, Bacillus bacteria and Aspergillus bacteria. Fermented cabbage extract of the present invention can be used as an active ingredient of the cosmetic composition because it maintains skin moisturizing homeostasis and hardly irritates the skin.

Description

Moisturizing enhancement cosmetic composition containing a fermented cabbage extract TECHNICAL COMPOSITION FOR MOISTURIZING SKIN CONTAINING FERMENTATIVE EXTRACT OF BRASSICA OLERACEA VAR. CAPITATA}

The present invention relates to a method for improving skin moisturizing cosmetic composition and moisturizing by containing fermented cabbage as an active ingredient.

Skin forms a major barrier from the outside environment and plays an important role in protecting our bodies from wounds, infections, water loss and UV rays. Ultraviolet light is one of the major causes of skin damage and induces skin aging. In the field of skin research, much research has been conducted on the relationship between ultraviolet light and skin.

Skin moisturizer has a barrier function that protects the body from various external stimuli and prevents moisture from inside, and develops into a thin stratum corneum containing moisture through the process of differentiation in the epidermal layer of the skin for moisture barrier function. Done. Structurally, the cells of the stratum corneum act like bricks, and the intercellular lipids of the keratinocytes act as a mortar, forming the skin barrier.

However, as people age, dry skin tends to increase, and the need for skin moisturizers increases due to indoor heating and air pollution. To this end, polyhydric alcohols and sugars as skin moisturizers have been used in cosmetics as general moisturizers. However, there is a drawback that the stickiness occurs on the skin, there is a problem that does not give sufficient moisturizing effect to the skin, and also does not give an intelligent moisturizing effect. In addition, in the summer when the humidity is very high, rather than draw moisture from the outside, the skin is sticky, in the winter, the humidity of the outside atmosphere is low, rather draw the moisture from the skin, rather the problem of drying the skin occurs.

Human skin is very sensitive to relative humidity and typically feels comfortable when the relative humidity is between 40 and 70%. Relative humidity is the ratio of the amount of saturated water vapor at a temperature equal to the amount of water vapor contained in 1 m ³ of air. In Korea, the humidity is high due to high humidity in summer and the air is very dry due to low relative humidity in winter. Human skin is also affected by seasonal changes and changes in humidity. In summer, when the temperature is high and high, the degree of discomfort increases in the summer when the relative humidity is high. I feel it. This means that when the relative humidity is high, it absorbs moisture from the air and becomes sticky. On the contrary, when the relative humidity is low, the moisture of the skin evaporates to dry. In particular, when the relative humidity is low, the dryness of the skin is often accompanied by pulling and itching.

Various cosmetic compositions have been trying to improve the dryness of the skin by using various moisturizing agents such as oils, sugars, polyols to overcome the dryness of the skin. For example, skin components such as ceramide, cholesterol, and fatty acids are used to enhance skin barrier function, thereby inhibiting moisture evaporating from the skin or using sugars, polyols, etc., which have strong ability to bind with water on the skin surface. To enhance the moisturizing effect of the skin. However, in the winter when the relative humidity is low, it is difficult to sustain the effect for a long time, and in the summer when the relative humidity is high, rather uncomfortable, there was a difficulty in preparing the appropriate cosmetic composition according to the change in the relative humidity.

Korean Patent No. 10-1424180 relates to an anti-inflammatory or skin moisturizing pharmaceutical composition containing a plant fermentation extract obtained by mixing and fermenting apple, carrot, radish, cabbage and the like.

Accordingly, the present inventors have repeatedly studied to prepare a cosmetic composition that maintains the skin's moisturizing power regardless of the change of seasons and the relative humidity and also maintains the effect for a long time. The present invention was completed by confirming that it can exhibit a continuous moisturizing effect regardless of the change in relative humidity.

Accordingly, it is an object of the present invention to provide a cosmetic composition for moisturizing the skin that provides continuous moisturizing power regardless of the change in relative humidity. Other objects and advantages of the present invention will become apparent from the following detailed description and claims.

The present invention relates to a moisturizing cosmetic composition containing a fermented cabbage extract. Specifically, the fermented cabbage extract is fermented with one or more bacteria selected from the group consisting of yeast, lactic acid bacteria, acetic acid bacteria, Bacillus bacteria and Aspergillus bacteria.

Preferably, the yeast is at least one selected from the group consisting of Saccharomyces cerevisiae, Schizosaccharomyces ellipsoideus and Saccharomyces boulardii.

Preferably, the lactic acid bacteria is Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus bulgaricus, Lactobacillus bulgaricus, Lactobacillus lamnosus (Lactobacillus rhamnosus), Lactobacillus tarlacus (Lactobacillus Lactobacillus) plantarum, Lactobacillus reuteri, Lactobacillus confusus, Lactobacillus fermentum, Lactobacillus brevis, and Termus thermomophilus (Thermus) It is 1 or more types chosen from group.

Preferably, the acetic acid bacterium is one or more selected from the group consisting of Acetobacter xylinum, Acetobacter pasteurianus, and Acetobacter hansenii.

Preferably, the Bacillus bacteria is 1 selected from the group consisting of Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium, and Bacillus mesentericus. It is more than a species.

Preferably, the Aspergillus bacterium is one or more selected from the group consisting of Aspergillus usami and Aspergillus oryzae.

Preferably, the cosmetic composition has a skin irritant effect.

The present invention provides a cosmetic composition containing the fermented cabbage extract as an active ingredient. Fermented cabbage extract of the present invention can be used as an active ingredient of the cosmetic composition because it maintains skin moisturizing homeostasis and hardly irritates the skin.

Figure 1 shows the result of the transdermal moisture loss amount of the cosmetic composition according to the present invention.

In the following description of the present invention, when it is determined that detailed descriptions of related well-known technologies or configurations may unnecessarily obscure the subject matter of the present invention, the detailed description will be omitted. The terms to be described below are terms defined in consideration of functions in the present invention, which may vary according to intentions or customs of users or operators, and the definitions should be made based on the contents throughout the specification for describing the present invention. .

The present invention relates to a cosmetic composition for moisturizing enhancement containing fermented cabbage extract. Fermented cabbage extract according to the present invention does not show cytotoxicity, and may exhibit a moisturizing improvement effect.

Cabbage ( Brassica oleracea var. Capitata), which belongs to the annual or biennial mustard of the dicotyledonous plant, has been shown to have physiological activities such as antioxidant, antibacterial or anticancer, and its efficacy and effectiveness have been verified through various clinical experiments.

In particular, the vesicles of mustard vegetables including cabbage accumulate glucosinolate, a secondary metabolite. Glucosinolate can be hydrolyzed by the myrosinase present in the cytoplasm and converted to isothiocyanate-based, nitrile-based, indole-based, and the like. Although some studies have been conducted on their physiology, nutrition or activity, studies on their skin moisturizing effects are insufficient.

Cabbage used in the present invention can be used for all parts of the cabbage.

Fermented cabbage extract used in the present invention means the fermented cabbage extracted. Specifically, the fermented cabbage extract means that the cabbage is preferably fermented with one or more bacteria selected from the group consisting of yeast, lactic acid bacteria, acetic acid bacteria, Bacillus bacteria and Aspergillus bacteria.

For example, the yeast may be used by selecting one or more from the group consisting of Saccharomyces cerevisiae, Schizosaccharomyces ellipsoideus and Saccharomyces boulardii. .

For example, lactic acid bacteria Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus bulgaricus, Lactobacillus rhamnosus, Lactobacillus rhamnosus, Lactobacillus Lactobacillus plantrum, Lactobacillus reuteri, Lactobacillus confusus, Lactobacillus fermentum, Lactobacillus fermentum, Lactobacillus brevis, and Termus thermophilus (Thermus thermophillus) One or more selected from may be selected and used.

As an example, acetic acid bacteria may utilize microorganisms in the genus Acetobacter. Specifically, at least one selected from the group consisting of acetobacter xylinum, acetobacter pasteurianus, and acetobacter hansenii may be used.

In one example, the Bacillus bacteria is selected from the group consisting of Bacillus subtilis, Bacillus rickeniform, Bacillus megaterium, and Bacillus mesentericus. Can be used.

For example, the Aspergillus bacteria may be used by selecting one or more from the group consisting of Aspergillus usami, and Aspergillus oryzae.

As the fermentation broth according to the present invention, fermentation broth itself, dried fermentation broth, filtrate of fermentation broth, centrifuge of fermentation broth or purified products of these substances may be used, but is not limited thereto.

The cosmetic composition of the present invention contains the fermented cabbage extract to the extent that it can maintain skin moisturizing homeostasis. The content of the fermented cabbage extract may be 0.0001 to 50.0% by weight, preferably 0.001 to 30.0% by weight relative to the total weight of the cosmetic composition. When the content of the fermented cabbage extract is less than 0.0001% by weight based on the total weight of the cosmetic composition, there is a problem that the skin moisturizing effect is slightly insufficient, and when the content of the fermented cabbage extract exceeds 50% by weight based on the total weight of the cosmetic composition There is a problem that increase the stability of the formulation, skin sensitivity with increasing.

Preferably, the fermented cabbage extract of the present invention may be prepared as follows.

a) preparation step such as powdering or extracting cabbage suitable for fermentation;

b) fermenting the resultant of step a) by using one or more bacteria selected from the group consisting of yeast, lactic acid bacteria, acetic acid bacteria, Bacillus bacteria and Aspergillus bacteria.

More preferably, c) the step of b) the fermentation of the fermentation may further comprise the step of low temperature.

Specifically, in step a), the plants may be powdered to be suitable for fermentation, or the plants may be extracted to be suitable for fermentation according to a conventional method, and the powder or extract may be mixed with purified water and used for fermentation. For example, the extract may be extracted with an organic solvent such as distilled water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, concentrated under reduced pressure, and dried to obtain a primary extract. More specifically, commercially available cabbage is pulverized, and 50 minutes in an extractor equipped with a reflux condenser with an organic solvent such as 5 to 20 volumes of water or an anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms based on the dry weight of the ground product. The extract of the plants is extracted by heating to 1 to 5 hours to 100 ℃. Then, after filtering with a filter cloth, the residue is further extracted one or more times in the same manner, the extracts are concentrated under reduced pressure, lyophilized or spray-dried to obtain a dry extract.

In step b), the resultant of step a) is autoclaved and inoculated with one or more bacteria selected from yeast, lactic acid bacteria, acetic acid bacteria, Bacillus bacteria or Aspergillus bacteria. Then in the fermenter 20 to 40 ℃, pH 4 to 7 conditions, preferably for 7 days to 28 days, the fermentation product is obtained by vacuum sterilization, centrifugation and filtration. The time required for the fermentation may vary depending on the fermentation temperature, and the fermentation conditions may vary depending on the condition of the plant, and thus, the content of the present invention is not limited to the above-described fermentation temperature, pH of the culture solution and fermentation time.

In step c), the fermented product obtained in step b) is preferably aged for 10 to 30 days at a humidity condition of 5 to 20 ° C. and 75 to 85%, and the time taken for the aging may vary. no.

The components contained in the cosmetic composition of the present invention include components commonly used in cosmetic compositions in addition to the fermented cabbage extract, for example, conventional auxiliaries such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavors, carriers and the like. It includes.

Cosmetic compositions of the present invention may be prepared in any formulation conventionally prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps and surfactant-containing cleansing, etc. It may be formulated as, but is not limited thereto. More specifically, in the formulation of flexible lotion, nutrition lotion, milk lotion, nutrition cream, massage cream, essence, eye cream, cleansing lotion, cleansing cream, cleansing foam, cleansing water, powder wash, cleansing oil, mask and pack Can be prepared.

When the formulation of the present invention is a paste, cream or gel, the carrier component is animal oil, vegetable oil, ester oil, wax, paraffin, starch, tracant, cellulose derivative, carbomer, polyacrylamide, polyethylene glycol, silicone, bentonite, Silica, talc or zinc oxide and the like can be used.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 And 3-butylene glycol, oils, glycerol aliphatic esters, fatty acid esters of polyethylene glycol or sorbitan, and the like.

When the dosage form of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Microcrystalline cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, an isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide ether. Sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

The cosmetic composition containing the fermented cabbage extract of the present invention is a cosmetic that helps maintain skin moisturizing homeostasis, and has excellent skin safety because of low irritation to the skin. This fact is specified in detail in the preparation examples below.

Hereinafter, the present invention will be described in more detail with reference to production examples. These preparation examples are for illustrating the present invention more specifically, and the scope of the present invention is not limited by these preparation examples. In addition, the part,%, and ratio used by this manufacture example correspond to a basis of weight unless there is particular notice.

[ Production Example ] Preparation of Fermented Cabbage Extract

After washing and crushing the dry cabbage, 70% ethanol was added to 100 g / L, refluxed three times for 5 hours, cooled and filtered with Whatman # 4 filter paper. The filtered extract was concentrated under reduced pressure and lyophilized at 50 ° C. or lower, and the dried extract was inoculated with fermentation strain at 5 g after autoclaving. Fermentation strain, Preparation Example 1 is Saccharomyces cerevisiae belonging to the yeast, Preparation Example 2 is Lactobacillus acidophilus belonging to the lactic acid bacteria, Preparation Examples 3 and 4 belong to the yeast Saccharomyces cerevisiae and Lactobacillus acidophilus belonging to the lactic acid bacterium (Lactobacillus acidophilus) in 1: 1, Preparation Example 5, Aspergillus Usami (Aspergillus usami) belonging to Aspergillus, Example 6 is Bacillus subtilis belonging to Bacillus, Preparation Examples 7 and 8 are Aspergillus usami belonging to Aspergillus and Bacillus subtilis belonging to Bacillus 1: 1 used.

Then fermented in a fermenter for 14 days at 30 ℃, pH 5 conditions. After fermentation, the fermentation broth was vacuum sterilized at 85 ° C., followed by centrifugation to remove fermentation bacteria first, and then fermented products were filtered by Whatman # 4 filter paper and aged at 15 ° C. and 80% humidity for 20 days.

[ Comparative Production Example ] Preparation of Cabbage Solvent Extract

Comparative Preparation Examples 2 and 3 are cabbage solvent extracts. The cabbage solvent extract was chopped and extracted with 100 g of dry cabbage at reflux three times for 5 hours with an aqueous 70% (V / V) ethanol solution, and then cooled and filtered with Whatman # 4 filter paper. Each filtered extract was prepared by concentration under reduced pressure and freeze-drying at 50 캜 or lower.

[ Experimental Example  1] Cytotoxicity Test of Fermented Cabbage Extract

To determine the cytotoxicity of the fermented cabbage extract prepared above was confirmed whether the toxicity through MTT assay. MTT (3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyl-tetrazolium bromide (3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyl-tetrazolium The bromide) assay is one of the methods for determining cell growth. The MTT assay is a test that takes advantage of the ability of the mitochondria to reduce the water-soluble yellow tetrazolium salt MTT to a blue-violet water-insoluble formazan crystal in living cells. When the cell viability is 95% or more, the cell is stable to the cell, and thus, the method is suitable for confirming the proper use concentration. Inoculate keratinocytes in 48-well plates at 1 × 10 ⁵ cells / well per well and incubate for 24 hours in 1.0 ml of DMEM (Dulbecco Minimum Essential Medium) medium. Was incubated for another 24 hours. The cultured plate was added with a 0.5 mg / ml MTT solution (Sigma, USA) 4 hours later, the medium was removed, and the cells were lysed with 1.0 ml of DMSO solution. ), The absorbance at 570 nm was measured. Using the untreated extract as a control group, the proliferation rate of the cells was calculated according to Equation 1 below, and the results are shown in Table 1 below.

Cabbage / fermented cabbage extract
content Cell proliferation (%) Comparative Production Example # 1 No addition 100.0 Comparative Production Example # 2 Cabbage Extract (10.0 wt%) 101.2 Comparative Production Example # 3 Cabbage Extract (20.0 wt%) 101.7 Preparation Example 1 Fermented Cabbage Extract (10.0 wt%)
/ Yeast fermentation 101.3 Preparation # 2 Fermented Cabbage Extract (10.0 wt%)
Lactic acid bacteria fermentation 101.4 Preparation # 3 Fermented Cabbage Extract (10.0 wt%)
Yeast + Lactic Acid Bacteria Fermentation 101.8 Preparation # 4 Fermented Cabbage Extract (20.0 wt%)
Yeast + Lactic Acid Bacteria Fermentation 101.7 Preparation # 5 Fermented Cabbage Extract (10.0 wt%)
Aspergillus 102.2 Preparation # 6 Fermented Cabbage Extract (10.0 wt%)
Bacillus bacteria 103.2 Preparation # 7 Fermented Cabbage Extract (10.0 wt%)
Aspergillus + Bacillus bacteria fermentation 102.3 Preparation Example # 8 Fermented Cabbage Extract (20.0 wt%)
Aspergillus + Bacillus bacteria fermentation 104.2

As can be seen from the results of Table 1, the fermented cabbage extract of the present invention does not exhibit cytotoxicity to keratinocytes, the composition containing the fermented cabbage extract did not affect the cell survival rate (all 100% or more) As a result, it can be confirmed that the fermented cabbage extract of the present invention is quite safe against cytotoxicity.

[ Example  And Comparative Example  Produce]

To prepare the examples and comparative examples as shown in Table 2.

(Unit: weight%) Classification Raw material name Comparative Example 1 Comparative Example 2 Comparative Example 3 Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Example 7 Example 8 A phase Purified water Remaining amount Remaining amount Remaining amount Remaining amount Remaining amount Remaining amount Remaining amount Remaining amount Remaining amount Remaining amount Remaining amount glycerin 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 Dipotassium glycyrinate 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 Disodium ID 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 Hydroxyethyl Cellulose 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 Dipropylene glycol 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 B phase antiseptic Quantity Quantity Quantity Quantity Quantity Quantity Quantity Quantity Quantity Quantity Quantity Squalane 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 Polyoxyethylene HydroxyCaster Oil 1.1 1.1 1.1 1.1 1.1 1.1 1.1 1.1 1.1 1.1 1.1 Sodium Pie 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Glyceryl Stearate 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Polyglyceryl-3methylglucose distearate 1.2 1.2 1.2 1.2 1.2 1.2 1.2 1.2 1.2 1.2 1.2 C phase Spices Quantity Quantity Quantity Quantity Quantity Quantity Quantity Quantity Quantity Quantity Quantity Pigment a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount Fermented Cabbage Extract
(Production example) - - - Preparation Example 1
10.0 Preparation Example 2
10.0 Preparation Example 3
10.0 Preparation Example 4
20.0 Preparation Example 5
10.0 Preparation Example 6
10.0 Preparation Example 7
10.0 Preparation Example 8
20.0 Cabbage Solvent Extract
(Comparative Production Example) - 10.0 20.0 - - - - - - - - Sum 100 100 100 100 100 100 100 100 100 100 100

[ Experimental Example  2] Moisturizing Measurement by Fermented Cabbage Extract

26 adult men and women who complained of dryness or dry skin were to apply the cosmetic composition of Comparative Examples 1 to 3 and Examples 1 to 8 twice daily for 4 weeks on the inside of Habak. Moisturizing power was compared by measuring Trans Epidermal Water Loss (TEWL) at constant temperature and humidity conditions (24 ° C., 40% relative humidity) at two and four weeks after application. Specifically, the process was performed under the following conditions, and the results are shown in FIG. 1.

<TEWL measurement method>

Principle: The amount of moisture evaporated from the skin is calculated according to the area and time, and the amount of moisture evaporated is read and quantified by the temperature and moisture electronic sensors to measure the moisture of the skin.

1. Environmental conditions: The temperature and humidity are kept constant and only a minimum of personnel (except as possible to the flow of air) are left in addition to the meter and the subject.

2. Place the TEWL probe on the subject's measurement site.

3. Do not apply excessive force to the measuring area (so as to leave marks on the skin) and place it lightly at an angle of 90 degrees to the ground.

4. Measure the value of the skin's TEWL level over time. (Usually 1-3 minutes)

5. Hold the probe handle as far as possible to prevent the body temperature from being transferred.

6. Calculate the amount of water evaporated per hour per area (g / m ² / h).

As can be seen in Figure 1, Comparative Examples 2 and 3 containing the cabbage solvent extract did not show a specific efficacy / effect in the water evaporation amount, but when used for 4 weeks in Examples 1 to 8 fermented cabbage extract Sufficient significant difference was shown. In addition, when the fermented cabbage extract (Examples 5 to 8) fermented with Aspergillus and Bacillus bacteria compared to the fermented cabbage extract (Examples 1 to 4) fermented by yeast and lactic acid bacteria, the same content is applied to the skin. It was confirmed that it shows an excellent moisturizing effect at, and it was confirmed that it shows an excellent effect on the moisturizing effect in a concentration dependent manner.

[ Experimental Example  3] HaCaT In keratinocytes  IL- 1α  Secretion inhibitory effect

37 ° C humidified by inoculating HaCaT keratinocytes (German Cancer Research Institute, Germany) with D0EM (Dulbeco's Modified Eagle's Medium, Gibco) with 10% FBS at a density of 2 x 10 ⁵ cells / well , 1 day incubation in a 5% CO ₂ incubator. After exchanging with serum-free DMEM, the samples of Comparative Preparation Examples 1 to 3 and Preparation Examples 1 to 8 were all treated with 100 ppm for 1 day of incubation and treated with SDS (sodium dodecyl sulfate, Sigma) for 4 hours. It was further cultured. After 4 hours, a portion of the culture medium was taken and used for quantification of IL-1α and protein. IL-1α quantification was performed according to the IL-1α quantification method of the kit using Endogen's human IL-1α kit. The amount of IL-1α secretion per protein was measured. As a positive control, Ketopropen was used as a skin irritant. In this experiment, the biosynthesis of IL-1α, which can cause erythema, which can occur on the skin through SDS treatment, was measured. After calculating the amount of -1α produced, it is a percentage of it. Final IL-1α production rate (%) was calculated by the following Equation 2, the results are shown in Table 3 below.

sample IL-1α SDS only processing 100.00 Comparative Production Example # 1 92.41 ± 1.67 Comparative Production Example # 3 91.93 ± 5.40 Preparation Example 1 91.50 ± 4.80 Preparation # 2 88.22 ± 5.10 Preparation # 3 86.32 ± 3.60 Preparation # 4 85.28 ± 3.66 Preparation # 5 83.33 ± 3.66 Preparation # 6 81.22 ± 3.30 Preparation # 7 80.30 ± 3.06 Preparation Example # 8 75.22 ± 3.30 KETOPROPEN 63.15 ± 7.96

According to the results of Table 3, unlike the cabbage solvent extract, it was found that the amount of IL-1α secreted in keratinocytes after SDS treatment in the fermented cabbage extract was significantly reduced compared to other samples. Therefore, it can be said that the stimulating alleviation component contained in the fermented cabbage extract reduces the amount of IL-1α to alleviate troubles such as erythema that may occur in the skin.

Claims (8)

A skin moisturizing cosmetic composition containing as an active ingredient a fermented cabbage extract fermented with a mixture of Aspergillus and Bacillus in a weight ratio of 1: 1. The method of claim 1,
The Bacillus bacteria is one or more selected from the group consisting of Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium, and Bacillus mesentericus. , Skin moisturizing cosmetic composition. The method of claim 1,
The Aspergillus bacteria is one or more selected from the group consisting of Aspergillus usami (Aspergillus usami), and Aspergillus duckwood (Aspergillus oryzae), cosmetic composition for skin moisturizing. The method of claim 1,
The cosmetic composition has a skin irritation relief effect, skin cosmetic composition. delete delete delete delete

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