KR20220165003A - Cosmetic Composition For Preventing Hair Loss Containing Fatty acids isolated from Malva verticillata L. Seed Extract - Google Patents

Abstract

The present invention relates to a cosmetic composition for preventing hair loss containing fatty acids isolated from a Malva verticillata L. seed extract as active ingredients and, specifically, to a cosmetic composition for preventing hair loss comprising linoleic acid, oleic acid and palmitic acid isolated from a Malva verticillata L. seed extract as active ingredients. The cosmetic composition for preventing hair loss has a dermal papilla cell proliferation effect, a hair growth signaling (β-catenin level, VEGF, IGF-1) effect of cells, and a β-catenin expression increasing effect.

Description

Cosmetic Composition For Preventing Hair Loss Containing Fatty Acids Isolated from Malva verticillata L. Seed Extract}

The present invention relates to a cosmetic composition for preventing hair loss containing, as an active ingredient, a fatty acid isolated from a Malva verticillata L. extract.

In general, the function of hair serves to protect the human body from external stimuli such as protecting the scalp and maintaining body temperature. Human hair is about 100,000 to 150,000, and it is formed in the hair follicle. Each hair has a different cycle and grows and falls out through a growth phase, a regression phase, and a resting phase. This cycle is repeated over 3 to 6 years, and an average of 50 to 100 hairs per day are normally lost.

The hair cycle is transformed and shortened by internal and external factors such as hormones, stress, disease, environmental pollution, and smoking. do.

Alopecia refers to an abnormally large number of hairs falling out due to a shortened growth period hair ratio and an increase in catagen or telogen hairs during this cycle, and hair loss symptoms can be suspected when more than 100 hairs fall out.

Clinically, hair loss is classified into scar-forming hair loss and non-scarring hair loss, and although it is caused by various causes, genetic causes and male hormone testosterone are considered important factors.

Testosterone is involved in skeletal muscle growth, male external genitalia, scrotum growth, spermatogenesis, etc. Testosterone is reduced to dihydrotestosterone by a reductase and is known to be involved in hair loss, acne, and prostate enlargement. (Diane et al; JID 775 -778, 1995. Bruchovsky, Net al; JBC 243,2112-2121, 1968).

More specifically, dihydrotestosterone is known to induce hair loss by reducing the proliferation of hair follicle cells by lowering protein synthesis around hair follicles.

Until now, treatments known to be effective in preventing hair loss or promoting hair growth include applying a minoxidil solution or taking trichosaccharide. Minoxidil was developed as a vasodilator for the treatment of hypertension, but as hirsutism was reported as a side effect, it was developed as a hair growth agent. Although the mechanism of action for the hair growth effect of minoxidil has not yet been clearly identified, it is believed that hair growth is induced by an increase in nutrient supply through vasodilation. In addition, finasteride (finasteride) is a substance that inhibits the activity of 5 alpha-reductase, an enzyme that acts on male hormone metabolism, and was initially developed as a treatment for prostatic hyperplasia, but was developed as a hair growth agent as hirsutism was reported as a side effect.

However, minoxidil and finasteride are inconvenient to continue to use because the effect disappears when use is stopped. In case of minoxidil, side effects such as sticky feeling and skin irritation have been reported, and in case of finasteride, sexual dysfunction such as energy loss and erectile dysfunction It has been reported that there are restrictions on continuous use.

Korean Patent Registration No. 10-131097 discloses an herbal extract for preventing hair loss and a hair product composition containing the same. Republic of Korea Patent Registration No. 10-121834 discloses a composition containing a plant extract or fraction such as Donggyuja, its use, and a method for preparing the extract.

An object of the present invention is to search for various skin physiological activities of natural plants and extracts thereof for hair loss relief and hair growth promotion, and as a result, the fatty acid isolated from the Malva verticillata L. extract has a dermal papilla cell proliferation effect related to hair loss relief, The present invention was completed by confirming the effect of cellular hair growth signaling (β-catenin level, VEGF, IGF-1).

The above object is achieved by a cosmetic composition for preventing hair loss comprising linoleic acid, oleic acid and palmitic acid, which are fatty acids isolated from the extract of Donggyuja, as active ingredients.

Preferably, the cosmetic composition may contain 0.5 to 2.5% by weight of linoleic acid, 0.5 to 1.5% by weight of oleic acid, and 0.5 to 1.5% by weight of palmitic acid based on the total weight of the cosmetic composition.

Also preferably, the linoleic acid, oleic acid and palmitic acid may be mixed in a weight ratio of 2:1:1.

Also preferably, the cosmetic composition has a dermal papilla cell proliferation effect, a cell hair growth signaling (VEGF, IGF-1) regulatory effect or an effect of increasing β-catenin (β-catenin) expression.

The cosmetic composition of the present invention contains the fatty acid isolated from the extract of Donggyuja as an active ingredient and has an effect of preventing hair loss. The cosmetic composition for preventing hair loss has a dermal papilla cell proliferation effect and a cell hair growth signaling (β-catenin level, VEGF, IGF-1) regulation effect.

1 is an analysis of linoleic acid, oleic acid, and palmitic acid separated from the methylene chloride fraction of Donggyuja extract using high performance liquid chromatography.
Figure 2 shows the dermal papilla cell proliferation rate of the Donggyuja extract of Preparation Example 1 and each or mixture of linoleic acid, oleic acid and palmitic acid in Preparation Examples 3 to 18.
Figures 3 and 4 show the hair growth factor (IGF-1, VEGF) expression promoting effect of the Donggyuja extract of Preparation Example 1 and each or mixture of linoleic acid, oleic acid and palmitic acid of Preparation Examples 3 to 18.
Figure 5 shows the β-catenin expression promoting effect of Donggyuja extract of Preparation Example 1 and each or mixture of linoleic acid, oleic acid and palmitic acid of Preparation Examples 3 to 18.

All technical terms used in the present invention, unless otherwise defined, have the following definitions and correspond to the meanings commonly understood by those of ordinary skill in the art related to the present invention. In addition, although preferred methods or samples are described in this specification, those similar or equivalent thereto are also included in the scope of the present invention.

The term "about" means a reference amount, level, value, number, frequency, percentage, dimension, size, amount, weight or length of 30, 25, 20, 15, 10, 9, 8, 7, 6, 5, means an amount, level, value, number, frequency, percentage, dimension, size, quantity, weight or length that varies by 4, 3, 2 or 1%.

Throughout this specification, unless the context requires otherwise, the terms "comprise" and "comprising" include a given step or element, or group of steps or elements, but any other step or element, or It is to be understood that steps or groups of components are not excluded.

The present invention relates to a cosmetic composition for preventing hair loss comprising fatty acids, particularly linoleic acid, oleic acid, and palmitic acid isolated from an extract of Donggyuja, as an active ingredient.

Donggyuja used in the present invention is a seed of mallow, an annual herbaceous plant belonging to the Malvaceae family, and grows naturally in northern temperate and subtropical climates, and grows wild in various parts of Korea. In Korea, it has been cultivated and used as an edible vegetable for a long time. Donggyuja, which is a seed, has been known for its diuretic, sluggish, oily, and detoxifying effects.

The fatty acids isolated from the extract of Donggyuja of the present invention are saturated or unsaturated carboxylic acids with long aliphatic chains. Fatty acids are generally present in living organisms and are used as energy sources in animals or as important structural components in cells. Fatty acids are classified according to chain length or number of unsaturated carbon atoms. Commonly known saturated fatty acids include myristic acid, palmitic acid, and stearic acid, and unsaturated fatty acids include myristic acid, oleic acid, linoleic acid, and linolenic acid.

The Donggyuja extract may be prepared according to a conventional method known in the art, that is, under conventional temperature and pressure conditions, using a conventional solvent, but preferably (a) water and 1-4 carbon atoms. Anhydrous or hydrous lower alcohols (e.g. methanol, ethanol, propanol and butanol), propylene glycol, 1,3-butylene glycol, glycerin, acetone, diethyl ether, ethyl acetate, butyl acetate, dichloromethane, chloroform, hexane Extraction is performed using a solvent extraction method using a solvent selected from the group consisting of and mixtures thereof, (b) supercritical extraction method using carbon dioxide under reduced pressure and high temperature, or (c) ultrasonic extraction method.

In the present invention, a solvent extraction method using an extraction solvent is preferably used. In the present invention, Donggyuja extract is prepared by using various extraction solvents, for example, water, anhydrous or hydrous lower alcohols having 1 to 4 carbon atoms (eg, methanol, ethanol, propanol and butanol), propylene glycol, 1,3-butylene glycol , It can be obtained using one or more extraction solvents selected from the group consisting of glycerin, acetone, diethyl ether, ethyl acetate, butyl acetate, dichloromethane, chloroform, hexane, and mixtures thereof, preferably ethanol, 70% (v/v) ethanol or water, most preferably 70% (v/v) ethanol.

On the other hand, it is obvious to those skilled in the art that the extract of Donggyuja of the present invention can be obtained using not only the above extraction solvent but also other extraction solvents that exhibit substantially the same effect. In addition, the extract of Donggyuja of the present invention includes not only the extract using the above-described extraction solvent, but also an extract that has undergone conventional purification and fermentation processes. For example, decompression by carbon dioxide, extraction by supercritical extraction by high temperature, extraction by ultrasonic extraction, separation using an ultrafiltration membrane with a constant molecular weight cut-off value, fractionation using an organic solvent, various chromatography (size , charge, hydrophobicity, or affinity), or the active fraction obtained through various additional purification and extraction methods, such as extraction by fermentation products using natural conditions or various microorganisms, are also of the present invention. included in the extract.

The fraction of the extract may be obtained by adding any one organic solvent selected from the group consisting of hexane, methylene chloride, ethyl acetate and butanol, and more specifically, may be obtained by adding methylene chloride.

In a specific embodiment of the present invention, it was confirmed that the hexane fraction, the methylene chloride fraction, the ethyl acetate fraction, and the butanol fraction obtained from the Donggyuja extract exhibited an excellent hair loss alleviating effect by exhibiting activity in dermal papilla cell proliferation, and the methylene chloride fraction had a hair loss alleviating effect It was confirmed that it is better.

According to one embodiment of the present invention, the fatty acid isolated from the extract of Donggyuja may include linoleic acid, oleic acid and palmitic acid isolated from the methylene chloride fraction of the extract of Donggyuja. Specifically, the fatty acids isolated from the Donggyuja extract refer to linoleic acid, oleic acid, and palmitic acid, and the fatty acids may be contained in an amount of 0.01% to 10% by weight based on the total weight of the cosmetic composition.

The linoleic acid has a structure represented by Formula 1 below.

[Formula 1]

The oleic acid has a structure represented by Formula 2 below.

[Formula 2]

The palmitic acid has a structure represented by Formula 3 below.

[Formula 3]

Preferably, the cosmetic composition according to the present invention contains 0.5% to 2.5% by weight of linoleic acid, 0.5% to 2.5% by weight of oleic acid, and 0.5% to 2.5% by weight of palmitic acid, based on the total weight of the cosmetic composition. can do. More preferably, it may contain 0.5 to 2.5% by weight of linoleic acid, 0.5 to 1.5% by weight of oleic acid and 0.5 to 1.5% by weight of palmitic acid.

According to one embodiment of the present invention, the linoleic acid, oleic acid and palmitic acid may be mixed in a weight ratio of 2:1:1.

The cosmetic composition according to the present invention includes linoleic acid, oleic acid, and palmitic acid separated from the extract of Donggyuja, and has a dermal papilla cell proliferation effect, a cell hair growth signaling (VEGF, IGF-1) regulatory effect, or β-catenin (β-catenin). ) has an effect of increasing expression.

Ingredients included in the cosmetic composition of the present invention may include ingredients commonly used in cosmetic compositions in addition to the active ingredients as active ingredients, for example, antioxidants, stabilizers, solubilizers, vitamins, pigments, and fragrances. adjuvants, and carriers.

The cosmetic composition of the present invention can be prepared in any formulation conventionally prepared in the art, for example, a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing , It may be formulated as an oil, pack, massage cream and spray, etc., but is not limited thereto.

When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as a carrier component. can

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizing agent or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-butyl glycol oil, fatty acid esters of glycerol, polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystals Star cellulose, aluminum metahydroxide, bentonite, agar or tracanth and the like may be used.

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluorohydrocarbon, propane / May contain a propellant such as butane or dimethyl ether.

When the formulation of the present invention is surfactant-containing cleansing, as carrier components, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide ether Sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester may be used.

When the cosmetic composition of the present invention is a soap, surfactant-containing shampoo formulation, or surfactant-free shampoo formulation, it may be applied to the hair and scalp and then wiped off, removed, or washed with water. As specific examples, the soap is liquid soap, powder soap, solid soap, and oil soap, and the surfactant-free cleansing formulation includes, but is not limited to, a cleansing cream, a cleansing lotion, a cleansing water, and a cleansing gel.

Hereinafter, the present invention will be described in more detail through examples. These examples are only for explaining the present invention in more detail, and the scope of the present invention is not limited by these examples according to the gist of the present invention.

Preparation Example 1: Preparation of Donggyuja Extract

After washing 10kg of same-gyuja with clean water and drying it, it was pulverized into a certain size (100mesh), extracted with 70% (V/V) ethanol aqueous solution under reflux three times for 5 hours each, cooled, and filtered with Whatman #4 filter paper. 1.2 kg of Donggyuja extract was prepared using a concentrator.

Preparation Example 2: Separation of Methylene Chloride Fraction and Fatty Acids of Donggyuja Extract

0.6 kg of Donggyuja extract obtained in Preparation Example 1 was sequentially subjected to solvent fractionation in a conventional manner using hexane, methylene chloride, ethyl acetate and butanol, and then concentrated under reduced pressure to obtain 21.2 g of hexane fraction, 13.2 g of methylene chloride fraction, and ethyl acetate Fractions of 12.4 g and butanol fractions of 8.2 g were obtained.

The methylene chloride fraction of the Donggyuja extract obtained above was centrifuged at 8,000 rpm for 15 minutes to obtain the supernatant. This was purified by preparative high-performance liquid chromatography to separate linoleic acid, oleic acid, and palmitic acid. The quality of linoleic acid, oleic acid, and palmitic acid was confirmed in FIG. 1 using high-performance liquid chromatography.

Preparation Examples 3 to 18: Preparation of mixtures with different ratios of linoleic acid, oleic acid, and palmitic acid obtained from Donggyuja extract

In order to confirm the hair loss relief effect of linoleic acid, oleic acid, and palmitic acid obtained in Preparation Example 2, a mixture of a single fatty acid and each fatty acid at different mixing ratios was prepared and used. The ratio (%) of linoleic acid, oleic acid, and palmitic acid according to the preparation example of Donggyuja extract is shown in Table 1 below.

division Preparation Example 3 Production Example 4 Preparation Example 5 Preparation Example 6 Preparation Example 7 Preparation Example 8 Production Example 9 Production Example 10 linoleic acid
(Linoleic acid) 1.0 0 0 1.0 1.0 1.0 2.0 1.0 oleic acid
(Oleic acid) 0 1.0 0 1.0 1.0 2.0 1.0 2.0 palmitic acid
(Palmitic acid) 0 0 1.0 1.0 2.0 1.0 1.0 2.0 division Preparation Example 11 Production Example 12 Preparation Example 13 Production Example 14 Production Example 15 Production Example 16 Production Example 17 Preparation Example 18 linoleic acid
(Linoleic acid) 2.0 2.0 2.0 2.0 1.5 1.5 1.0 1.0 oleic acid
(Oleic acid) 1.0 2.0 1.0 1.5 2.0 1.0 1.5 1.5 palmitic acid
(Palmitic acid) 2.0 1.0 1.5 1.0 1.0 2.0 2.0 2.0

Experimental Example 1: Evaluation of dermal papilla cell proliferation

Human-derived dermal papilla cells (human follicle dermal papilla cells, HFDPC) were dispensed into a 96 well plate at 1 × 10 ⁴ cells/ml, and stabilized for 24 hours in a 37°C, 5% CO ₂ cell incubator. After exchanging it with a DMEM medium containing 1% FBS, the samples were diluted by concentration and reacted for 48 hours. Then, 20 μl of MTT reagent at a concentration of 5 mg/ml was added to each well and reacted for 2 to 3 hours in a 37° C., 5% CO ₂ cell incubator. After the reaction was completed, the supernatant of each well was removed, and 100 μl of DMSO was added to completely dissolve the cells, and the absorbance was measured at 550 nm. At this time, the positive control group used minoxidil, a hair growth promoting agent, and compared with the absorbance value of the control group, the cell proliferation rate of each sample group was calculated and shown in FIG. 2 .

The results of the dermal papilla cell proliferation evaluation test results for the Donggyuja extract and Preparation Examples 3 to 18 are shown in FIG. 2 . As shown in Figure 2, the dermal papilla cell proliferation effect was shown in all preparation examples including preparation example 1. In particular, in Preparation Example 9 in which linoleic acid 2: oleic acid 1: palmitic acid 1 was mixed, the proliferation rate of dermal papilla cells increased by about 27%, showing the most excellent effect.

Experimental Example 2: Hair growth factor expression promotion effect

Dermal papilla cells were dispensed into 96 wells at a concentration of 1 × 10 ⁵ cells/ml and cultured for one day at 37°C, 5% CO ₂ incubator, and then the samples were treated by concentration and reacted in a 37°C, 5% CO ₂ incubator for 24 hours. . It was measured by enzyme-linked immunosorbent assay (ELISA) using the cell culture medium.

The expression of IGF-1 (Insuline-like growth factor) and VEGF (Vascular endothelial growth factor) in the cell culture was analyzed using an ELISA kit, and the absorbance was taken at 450 nm, and the respective production amounts are shown in FIGS. 3 and 4 . At this time, the positive control group used minoxidil, a hair growth promoting agent.

As shown in Figures 3 and 4, the hair growth factor expression promoting effect was shown in Preparation Example 1 and Preparation Examples 3 to 18.

In particular, as shown in FIG. 3, in Preparation Example 6 in which linoleic acid 1 : oleic acid 1 : palmitic acid 1 was mixed and in Preparation Example 9 in which linoleic acid 2 : oleic acid 1 : palmitic acid 1 was mixed, the amount of IGF-1 produced was 26 %, showed the most excellent effect by increasing by 27%.

In addition, as shown in FIG. 4, it was confirmed that the production of VEGF increased the most to about 24% in Preparation Example 9 in which linoleic acid 2: oleic acid 1: palmitic acid 1 was mixed.

Experimental Example 3: Confirmation of Wnt/β-catenin expression rate

Dermal papilla cells were dispensed into 6 wells at a concentration of 2 × 10 ⁵ cells/well and cultured in a 37°C, 5% CO ₂ incubator for one day, and then the samples were treated by concentration and cultured for 24 hours. The cultured cells were collected and lysed, and proteins were quantified using BCA assay. The quantified proteins were electrophoresed on a 10% SDS-PAGE gel and then transferred to a PVDF membrane. After shaking the primary antibody (β-catenin, santa cruz, USA) overnight at 4°C, the HRP-conjugated secondary antibody was reacted at room temperature for 2 hours. After that, it was washed three times with 1 × TBST and the band was confirmed using an ECL reaction kit.

The intensity of each band was quantified based on the expression of β-actin to calculate the β-catenin expression rate. At this time, the positive control was shown in FIG. 5 using minoxidil, a hair growth promoter.

As shown in Figure 3, the expression of β-actin was promoted in Preparation Example 1 and Preparation Examples 3 to 18. In particular, in Preparation Example 6 mixed with linoleic acid 1: oleic acid 1: palmitic acid 1 and Preparation Example 9 mixed with linoleic acid 2: oleic acid 1: palmitic acid 1, the expression amount of β-actin increased by 51% and 49%, respectively. showed the best effect.

Formulation Examples 1 to 2: Preparation of Shampoo for Preventing Hair Loss Containing Donggyuja Extract

A formulation example of the composition according to the present invention containing 3% by weight of Preparation Example 1 containing 3% by weight of the Donggyuja extract obtained in Preparation Example 1 and Preparation Example 9 in which linoleic acid 2: oleic acid 1: palmitic acid 1 was mixed. The composition for preventing hair loss of Example 2 was described as the composition in the table below. It can be applied in various formulations, which is intended to be specifically described and not intended to limit the present invention.

In Formulation Examples 1 and 2, shampoos according to the contents of Table 2 below were prepared as compositions for preventing hair loss containing Donggyuja extract, but in more detail, softening lotion, nutrient lotion, nutrient cream, massage cream, essence, It may be formulated into an eye cream, shampoo, treatment, scalp ampoule, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.

ingredient Formulation Example 1 (% by weight) Formulation Example 2 (% by weight) Comparative Example 1 (% by weight) Preparation Example 1 3.0 - - Preparation Example 9 - 3.0 - Disodium EDT 0.03 0.03 0.03 Polyquaternium-10 0.1 0.1 0.1 Ammonium Lauryl Sulfate 40.5 40.5 40.5 Ammonium Laureth Sulfate 15.5 15.5 15.5 Cocamidopropyl Betaine 6.0 6.0 6.0 propylene glycol 2.0 2.0 2.0 sodium xylene sulfonate 2.0 2.0 2.0 Glycol Distearate 3.0 3.0 3.0 Cocamide FA 1.0 1.0 1.0 Lauramide FA 1.0 1.0 1.0 salicylic acid 0.13 0.13 0.13 Simethicone 3.0 3.0 3.0 antiseptic Appropriate amount Appropriate amount Appropriate amount incense Appropriate amount Appropriate amount Appropriate amount Purified water balance balance balance total 100.0 100.0 100.0

Experimental Example 1: Anti-hair loss effect of cosmetics

In Experimental Example 1, the hair loss prevention effect of the shampoo prepared in the formulation example was confirmed. After randomly dividing 20 adult males who recognized that they had hair loss into 2 groups (A, B) of 10 people each, the experiment was conducted for 4 weeks with 10 people in the experimental group (A) and 10 people in the control group (B). The composition of Formulation Example 1 was used for Group A, the composition of Formulation Example 2 was used for Group B, and the composition of Comparative Example 1 was used for Group C at 2-day intervals, respectively, and the results were observed. The degree of hair loss prevention was evaluated by the test subjects after the experiment was completed (-3: very worse, -2: worse, -1: slightly worse, 0: no change, 1: slightly improved, 2: improved, 3: very improved) According to the sensory evaluation, it is shown in Table 3 below.

subject Degree of hair loss prevention Group A group B C group One One 2 0 2 3 3 -3 3 One 2 0 4 2 3 -2 5 2 3 -One 6 0 One -3 7 3 3 0 8 3 2 -One 9 2 2 0 10 One One -2 Average 1.8 2.2 -1.2

As shown in Table 2, the composition of Formulation Example 2 was found to have an excellent hair loss prevention effect compared to the composition of Comparative Example 1.

Claims (4)

A cosmetic composition for preventing hair loss comprising linoleic acid, oleic acid and palmitic acid isolated from an extract of Donggyuja as active ingredients. The cosmetic composition for preventing hair loss according to claim 1, wherein the cosmetic composition contains 0.5 to 2.5% by weight of linoleic acid, 0.5 to 1.5% by weight of oleic acid, and 0.5 to 1.5% by weight of palmitic acid, based on the total weight of the cosmetic composition. The cosmetic composition for preventing hair loss according to claim 1, wherein the linoleic acid, oleic acid and palmitic acid are mixed in a weight ratio of 2:1:1. The method of claim 1, wherein the cosmetic composition is for preventing hair loss, characterized in that it has a dermal papilla cell proliferation effect, a cell hair growth signaling (VEGF, IGF-1) regulation effect or an effect of increasing the expression of β-catenin (β-catenin) cosmetic composition.

Images