KR20170005952A - Cosmetic composition comprising Malva verticillata Seed extract for prevention of skin aging or anti-wrinkles - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising a Malva verticillata seed extract for preventing skin aging or wrinkles. According to the present invention, the cosmetic composition comprising a Malva verticillata seed extract inhibits 11-HSD1 and MMP-1, thereby showing anti-aging or skin improvement effects.

Description

[0001] The present invention relates to a cosmetic composition for improving skin aging or skin wrinkles,

The present invention relates to a cosmetic composition for improving skin aging or skin wrinkles, and more specifically, to a cosmetic composition for improving skin aging or skin wrinkles containing an extract of Allium tuberosus.

Skin is the outermost organ of the body and plays a role in protecting our body from external environment. However, skin is more vulnerable to external stimuli than other organs, and can be easily attacked. In particular, facial skin is exposed directly to ultraviolet rays and pollutants, and thus is susceptible to premature aging.

The wrinkles, elasticity reduction, skin burning and drying phenomenon appearing on the skin due to ultraviolet rays, air pollution, stress, illness, smoking, etc. are mostly caused by changes of extracellular matrix proteins, Collagen, elastin, and hyaluronic acid. Especially, matrix metalloproteinases (MMPs) are known as the most important enzymes to degrade matrix proteins. The activity of MMP is greatly increased by a single ultraviolet irradiation, and it accelerates the formation of wrinkles by decomposing a plurality of substrate proteins including collagen.

Recently, new factors have been attracting attention for the change of skin tissue due to UV exposure. Skobowiat et al. Have confirmed that 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) plays a key role in ultraviolet-induced stress regulation (Skobowiat C et al, 2013; 168 (3): 595-601). 11β-HSD1 is an enzyme that converts costisol, a type of stress hormone, from cortisone, an inactive state, into cortisol, which is in an active state, and is distributed in epidermis and dermis. . In other words, it is a substance that increases activity and expression by external factors such as ultraviolet rays, air pollutants, and chemicals as well as stress responses due to various internal psychological factors, and is closely related to a stress reaction in skin. have.

In the meantime, vitamin C, α-tocopherol, retinol and derivatives thereof have been used in cosmetics and medicines for improving wrinkles. However, when they are formulated in the formulations, they have a disadvantage that they are not removed or chemical stability is poor have.

In order to overcome these disadvantages, the development of a cosmetic composition for wrinkle improvement using natural materials derived from natural materials has been attracting attention and needs to be discovered.

 Malva verticillata L. Seed is a dried herbaceous plant of the mugwort plant. It has little odor, is soft and greasy, has a sweet taste, and has a good quality. It has been known that it acts on the cysts and has been traditionally used in the elderly or the Huhan people due to depression, edema, dysuria, constipation, insufficient secretion of milk.

Conventionally, cosmetic compositions for skin aging or wrinkle improvement using various natural materials have been extensively studied. However, the effect of the extract of the present invention on skin aging or wrinkles is not yet known.

The inventors of the present invention have made extensive efforts to overcome the problems of the prior arts. As a result, in the case of a cosmetic composition for improving skin aging or skin wrinkles containing an extract of Allium tuberosus as an active ingredient, by inhibiting 11? -HSD1 and MMP- Aging, or skin-opening effect. Thus, the present invention has been completed.

KR 10-0825256 B KR 10-1393007 B

Accordingly, it is a main object of the present invention to provide a cosmetic composition for improving skin aging or skin wrinkles, which contains an extract of Phellinus extract having skin aging or skin improving effect by inhibiting 11? -HSD1 and MMP-1.

According to one aspect of the present invention, there is provided a cosmetic composition for skin aging or wrinkle improvement, which contains an extract of Aspergillus as an active ingredient.

Conventionally, vitamin C, alpha -tocopherol, retinol and derivatives thereof have been used in cosmetics and medicines as a material for improving wrinkles. However, they have been disadvantageous in that they are deteriorated when they are formulated in formulations or chemical stability is poor . Accordingly, the inventors of the present invention have made efforts to find out natural materials derived from natural materials as a material for improving wrinkles. As a result, it has been confirmed that the extract of the present invention has an effect of improving wrinkles.

In the present invention, the extract may be extracted with any solvent conventionally used, and preferably water, anhydrous or hydrous methanol, ethanol, propyl alcohol, butyl alcohol, glycerin, propylene glycol, butylene glycol, Or a mixed solvent of these solvents.

In the present invention, the extract of Alaska pollack is contained in an amount of 0.01 to 50% by weight based on the total weight of the composition. If it is out of the above range, formulation is not easy and it is not preferable.

In the present invention, the extracts of the present invention are characterized by exhibiting an effect of improving skin aging by inhibiting 11? -HSD1 (11? -Hydroxysteroid dehydrogenase type 1).

According to the experimental example of the present invention, it was confirmed that the extract of Lycopersicon esculentum of the present invention inhibited 11β-HSD1 increased by UVB. From these results, it can be seen that the extract of the present invention of the present invention can inhibit 11? -HSD1, thereby alleviating stress in the skin and improving skin aging (see Experimental Example 2-3).

In the present invention, the extracts of the present invention are characterized by exhibiting a wrinkle-reducing effect by inhibiting MMP-1 (Matrix metalloproteinase-1).

According to the experimental example of the present invention, it was confirmed that the extract of the present invention inhibited MMP-1 increased by UVB. From these results, it can be seen that the extract of the present invention inhibits the degradation of skin proteins such as collagen by inhibiting MMP-1, thereby improving wrinkles (see Experimental Example 1).

In accordance with the present invention, the extract of Phellinus linteus is characterized by exhibiting an effect of improving skin aging or wrinkles by increasing the expression of procollagen type I.

According to the experimental example of the present invention, the content of procollagen type I in the artificial cultured skin was decreased in the UVB-irradiated group, whereas in the artificial cultured skin irradiated with UVB, the content of procollagen type I was increased in the treated group . From these results, it can be seen that the extract of the present invention of the present invention can increase the expression of procollagen type I, thereby improving skin aging or wrinkles (see Experimental Example 4).

In the present invention, the composition may be used as a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, a nutritional cream, a moisturizing cream, a hand cream, , A soap, a cleansing foam, a cleansing lotion, a cleansing cream, a body lotion and a body cleanser.

INDUSTRIAL APPLICABILITY As described above, the cosmetic composition containing the extract of the present invention as an active ingredient effectively inhibits the expression of 11? -HSD1 and MMP overexpressed by ultraviolet rays, thereby relieving stress on the skin and improving aging and wrinkles Effect.

Brief Description of the Drawings Fig. 1 is a graph showing the results of experiments confirming the inhibitory activity of MMP-1 on the extract of Phellodendus japonica.
Fig. 2 is a graph showing the results of an experiment for confirming the inhibitory activity of 11? -HSD1 on the extract of P. japonica.
FIG. 3 is a graph showing the results of an experiment for confirming the inhibitory effect of Mongolian extract on MMP-1 in artificial cultured skin.
Fig. 4 is a graph showing the results of an experiment in which an extract of Phellinus linteus was confirmed to be able to grow procollagen type I in artificial cultured skin.

Hereinafter, the present invention will be described in more detail with reference to Examples. These embodiments are only for illustrating the present invention, and thus the scope of the present invention is not construed as being limited by these embodiments.

Example  1: Preparation of extract

The inventors used in the present invention were purchased and used in Dongbang, Korea.

100 g of copper oxide was taken in a separate container and immersed in 1 L of a 70% by weight aqueous ethanol solution as an extraction solvent and left at room temperature for 72 hours to obtain an extract. The obtained extract was filtered using a filter paper, and the filtrate was concentrated under reduced pressure using a rotary evaporator to obtain 10.0% of the extract. The obtained extract mixture was dissolved in DMSO (dimethyl sulfoxide) as a solubilizer at a concentration of 1 mg / ml and 10 mg / ml, and used in the following experimental examples.

Experimental Example  1: Extract of Phellinus linteus MMP -One Low performance  evaluation

The amount of MMP-1 expression in human dermal fibroblast (Hs68) was measured in order to evaluate the MMP-1 inhibitory effect of the extracts of Example 1.

Human fibroblasts were divided into 4 wells of ⁶ well plates and incubated for 24 hours in a 5% CO ₂ incubator at 37 ° C. After the medium was removed, DPBS was added, and the remaining cells except the UVB non-irradiated group were irradiated with UVB at 15 mJ / cm ² , and then the extracts were further added for 24 hours. RNA was isolated from the cells of each sample using tryzone (RNA iso, DAKARA, Japan), quantitated at 260 nm using nanodrop, and 2 μg of each RNA was used to amplify cDNA (C1000 Thermal Cycler, Bio-Rad, USA). Real-time PCR was performed in a real-time PCR machine using a mixture of the target protein MMP-1 primer and cyanine dye SYBR Green supermis (Applied Biosystems, USA). And the degree of expression of the MMP-1 gene was finally evaluated. The sequence of the template and the reaction conditions were as shown in Table 1 below, and the expression amount of the gene was finally analyzed by correcting for the β-actin gene. The results are shown in Fig.

Primer order Reaction conditions MMP-1 F 5'-CGAATTTGCCGACAGAGATGA-3 ' After activation of the polymerase at 94 ° C for 5 minutes, polymerization was carried out at 40 ° C for 30 seconds at 95 ° C for 30 seconds, at 55 ° C for 30 seconds, and at 72 ° C for 30 seconds R 5'-GTCCCTGAACAGCCCAGTACTT-3 ' Ll [beta] -HSDl F 5'-AAGCAGAGCAATGGAAGCAT-3 ' R 5'-GAAGAACCCATCCAAAGCAA-3 ' β-Actin F 5'-GGCCATCTCTTGCTCGAAGT-3 ' R 5'-GAGACCTTCAACACCCCAGC-3 '

As a result, as shown in FIG. 1, it was confirmed that the amount of MMP-1 gene expression was decreased in a concentration-dependent manner in the group treated with the same extract, while the amount of MMP-1 expression was increased in the UVB irradiation group.

Experimental Example  2: Expression of 11? HSD1 Low performance  evaluation

In order to evaluate the inhibitory effect of 11beta-HSD1 on the extract of Phellinus extract of Example 1, the same cells as in Experimental Example 1 were used to conduct experiments under the same conditions. Only 11β-HSD1 template was used in place of MMP-1 template in the final real-time PCR. The sequence of the template and the reaction conditions were as shown in Table 1, and the expression level of the gene was finally analyzed by correcting for the β-actin gene. The results are shown in Fig.

As a result, as shown in FIG. 2, it was confirmed that the amount of 11? -HSD1 gene was decreased in a concentration-dependent manner in the same manner as the MMP-1 gene expression amount in the same group treated with extracts of the same strain.

Experimental Example  3: Artificial culture of the extract MMP -One Low performance  evaluation

Experiments were conducted using EpiDermFT ™ (MatTek, USA), a three-dimensional artificial cultured skin tissue, in order to evaluate the MMP-1 inhibitory effect of the extract of Example 1 at the tissue level.

Epidermis was added to then stabilize the EpiDermFT ™ containing both the dermis for a primary to 24 hours in culture medium to remove the culture medium and after donggyuja extract the remaining experimental groups except the UVB non-adjustable sagun review of UVB of 60 mJ / cm ² at different concentrations The medium was replaced with another medium and cultured for additional 48 hours. Subsequently, the culture medium in which the artificial dermal tissue was cultured was collected, and the suspension was removed by centrifugation to measure the amount of protein contained in the culture medium. The amount of MMP-1 protein contained in the culture medium was measured by a guided method using an MMP-1 ELISA Kit (EMD Millipore, USA).

As a result, as shown in FIG. 3, the content of MMP-1 in the culture medium of the UVB-irradiated group was higher than that of the non-irradiated group, whereas the content of MMP-1 was decreased to the same level as that of the non-irradiated group .

Experimental Example  4: Artificial cultivation The extract procollagen type  Ⅰ Possible  evaluation

Experiments were carried out under the same conditions using the same tissues as in Experimental Example 3, in order to evaluate the effect of increasing the procollagen type I in the extract of P. japonica at the tissue level. Finally, the procollagen type Ⅰ content in the culture medium was measured using the Procollagen type Ⅰ C-peptide (PIP) EIA kit (Takara-bio Inc., Japan).

As a result, as shown in FIG. 4, the content of procollagen type Ⅰ in the culture medium of the UVB irradiation group was smaller than that of the non-control group, while the content of the same group was increased in the treatment group.

Formulation Example  1: Manufacture of Toner

The toner preparation containing the extract obtained in Example 1 was prepared by a conventional method according to the compositional components and composition ratios shown in Table 2 below.

ingredient Content (% by weight) Example 1 1.0 glycerin 3.0 Butylene glycol 2.0 Polyoxyethylene (60) Hardened castor oil 0.5 Polysorbate 80 0.5 ethanol 10.0 antiseptic a very small amount Spices a very small amount Purified water Balance

Formulation Example  2: Preparation of lotion agent

Lotion preparations containing the extract obtained in Example 1 were prepared according to the conventional methods according to the composition components and composition ratios shown in Table 3 below.

ingredient Content (% by weight) Example 1 1.0 glycerin 7.0 Butylene glycol 3.0 Glyceryl stearate / phage-100 stearate 1.0 Polysorbate 60 0.5 Sorbitan sesquioleate 0.5 Squalane 3.0 Caprylic / capric triglyceride 5.0 Sheer butter 2.0 Stearyl alcohol 0.5 Stearic acid 0.5 Carboxyl vinyl polymer 0.2 Arginine 0.2 antiseptic a very small amount Spices a very small amount Purified water Balance

Formulation Example  3: Manufacture of cream agent

The cream preparation containing the extract obtained in Example 1 was prepared according to the conventional method according to the composition components and composition ratios shown in Table 4 below.

ingredient Content (% by weight) Example 1 1.0 glycerin 7.0 Butylene glycol 5.0 Glyceryl stearate / phage-100 stearate 2.0 Polysorbate 60 0.5 Sorbitan stearate 0.5 Phitosqualan 5.0 Jojoba oil 3.0 Caprylic / capric triglyceride 10.0 Aloe butter 3.0 Wax 1.0 Stearyl alcohol 0.5 Beeryl alcohol 0.5 Stearic acid 0.5 Carboxyl vinyl polymer 0.2 Arginine 0.3 Dimethicone 0.5 antiseptic a very small amount Spices a very small amount Purified water Balance

Claims (7)

A cosmetic composition for improving skin aging or wrinkles containing an extract of Allium japonica as an active ingredient.
The method according to claim 1, wherein the extract is extracted with at least one solvent selected from the group consisting of water, anhydrous or hydrous methanol, ethanol, propyl alcohol, butyl alcohol, glycerin, propylene glycol, butylene glycol, Wherein the cosmetic composition for skin aging or wrinkle improvement is a composition for improving skin aging or wrinkles.
[Claim 2] The cosmetic composition for skin aging or wrinkles according to claim 1, wherein the extract is from 0.01 to 50% by weight based on the total weight of the composition.
The cosmetic composition for skin aging or wrinkles according to any one of claims 1 to 3, wherein the extract has a skin aging-improving effect by inhibiting 11? -HSD1 (11? -Hydroxysteroid dehydrogenase type 1).
The cosmetic composition for skin aging or wrinkles according to any one of claims 1 to 3, wherein the extract has a wrinkle-reducing effect by inhibiting MMP-1 (Matrix metalloproteinase-1).
The cosmetic composition for skin aging or wrinkles according to any one of claims 1 to 3, wherein the extract of Phellodendus japonica exhibits a skin aging or wrinkle reducing effect by increasing the expression of procollagen type I.
The composition according to claim 1, wherein the composition is at least one selected from the group consisting of a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, a nutritional cream, a moisturizing cream, a hand cream, Wherein the cosmetic composition is at least one selected from the group consisting of a pack, a soap, a cleansing foam, a cleansing lotion, a cleansing cream, a body lotion and a body cleanser.

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