WO2017007142A1

WO2017007142A1 - Cosmetic composition for alleviating skin aging or skin wrinkles, containing malva verticillata seed extract

Info

Publication number: WO2017007142A1
Application number: PCT/KR2016/006358
Authority: WO
Inventors: 박지은; 남진주; 노윤화; 김연준
Original assignee: 코스맥스 주식회사
Priority date: 2015-07-06
Filing date: 2016-06-15
Publication date: 2017-01-12
Also published as: KR101762111B1; KR20170005952A

Abstract

The present invention relates to a cosmetic composition for alleviating skin aging or skin wrinkles, containing a Malva verticillata seed extract. According to the present invention, the cosmetic composition containing a Malva verticillata seed extract as an active ingredient can exhibit an effect of alleviating skin aging or improving the skin by inhibiting 11β-HSD1 and MMP-1.

Description

【Specification】

[Name of invention]

Cosmetic composition for skin aging or wrinkle improvement containing copper sperm extract

The present invention has been made by the task number 10046945 under the support of the Republic of Korea Small and Medium Business Administration, the research management specialized agency of the project is Korea Industrial Technology Promotion Agency, the research project name is "World Class 300 Project R & D", the research project name is "K-Cosmetic leading functional Development of skin cell receptor-regulated functional new materials and development of optimal skin delivery technology containing them ", The host organization is Cosmax Co., Ltd., and the research period is 2013.09.01 ~ 2017.08.31.

The present invention relates to a skin composition for improving skin aging or wrinkles, and more particularly to a cosmetic composition for improving skin aging or wrinkles containing a copper sperm extract.

Background Art

The skin is the outermost organ of the body, protecting our bodies from the outside environment. However, the skin is exposed to external stimuli more easily than other organs, so it is easily attacked. In particular, the skin of the face is directly exposed to ultraviolet rays and pollutants, and thus has a characteristic of premature aging.

Wrinkles, decreased elasticity, skin sagging, and dryness caused by UV rays, air pollution, stress, illness, and smoking are mostly caused by changes in the extracelular matrix protein. The collagen, elastin, and hyaluronic acid, which are connective tissues, are considered to be the cause. In particular, matrix metal loproteinases (MMPs) are known as representative enzymes that degrade substrate proteins. MMP only one UV Irradiation also significantly increases its activity, accelerating wrinkle formation by degrading collagen and other matrix proteins.

A new factor has recently attracted attention for changes in skin tissues caused by UV exposure, and studies by Skobowiat et al. Confirmed that 11β-hydroxysteroid dehydrogenase type Κΐΐβ -HSDI plays a key role in the regulation of UV-induced stress. C et al, 2013; 168 (3): 595-601). 11β-HSD1 is an enzyme that converts cortisol, a stress hormone, from inactivated cortisone to activated cortisol, and is distributed in the epidermis and dermis. It was found that the expression increased significantly. In other words, it is not only stress reaction caused by various internal mental factors but also activity and expression is increased by external factors such as ultraviolet rays, air pollutants and chemicals. have.

Wrinkle improvement material has been mainly used in cosmetics and medicines, such as vitamin C, a-tocope, retinol and derivatives thereof, but when they are formulated in the formulation, there is a bad smell or poor chemical stability There are disadvantages.

In order to overcome these disadvantages, the development of cosmetic compositions for improving wrinkles using natural-derived materials are in the limelight and require the excavation thereof. Malva vert ici l lata L. Seed is a dried dried Mallow larva seed, a odorless, soft and greasy, sweet and cold. It is known to work on the cystoscope, it has been traditionally used in pregnancy, swelling, urination disorders, constipation, lack of lactation, the elderly and people with disabilities.

In the past, many studies have been made on skin aging or anti-wrinkle cosmetic compositions using various natural substances, but the effect of aging on the skin aging or wrinkle improvement of the present invention is not known.

Accordingly, the present inventors have diligently researched to overcome the problems of the prior art, in the case of a cosmetic composition for improving skin aging or wrinkles containing Donggyuja extract as an active ingredient, by inhibiting Ιβ-HSDl and MMP-1 It was confirmed that it can exhibit the skin aging or skin aging effect, and completed the present invention.

Detailed Description of the Invention

[Technical problem]

Therefore, the main object of the present invention is to provide a cosmetic composition for improving skin aging or wrinkles containing Donggyuja extract having skin aging or skin improvement effect by inhibiting ΙΙβ -HSDl and MMP-1.

Technical Solution

According to one aspect of the present invention, the present invention provides a cosmetic composition for improving skin aging or wrinkles, which contains an extract of Donggyuja as an active ingredient.

Conventionally, as a material for improving wrinkles, vitamins ⁽ ：, α-tocope, retinol and derivatives thereof have been used in cosmetics and medicines, but when they are formulated in the formulation, bad odor or chemical stability is poor. As a result of the present inventors' efforts to discover natural substances derived from the material for improving wrinkles, the present inventors confirmed that the extract of the present invention has an effect of improving wrinkles and completed the present invention.

In the present invention, the sperm extract may be extracted with any solvent used in the prior art, preferably extracted with water, hydrophilic oil-soluble solvents or their mixed solvents, more preferably water, anhydrous or water And methanol, ethane, propyl alcohol, butyl alcohol, glycerin, propylene glycol, butylene glycol, or one or more solvents selected from the group consisting of a mixed solvent thereof. All of them have a hydrophilic alcohol group (0Η), which shows similar polarity, and thus can exhibit similar extraction activity for solutes.

In the present invention, the sperm extract is characterized in that it comprises 0.01 to 50% by weight relative to the total weight of the composition. If it is outside the above range, the formulation is not available and is not preferable.

In the present invention, the sperm extract is ll | 3 -HSDl (lli3 -hydn) xysteroid By suppressing dehydrogenase type 1) is characterized in that the skin aging improvement effect.

According to the experimental example of the present invention, it was confirmed that the sperm extract of the present invention inhibits ΙΙβ -HSDl increased by UVB. From these results, it can be seen that the sperm extract of the present invention can improve skin aging by relieving stress in the skin by inhibiting ΙΙβ-HSDl (see Experimental Example 2-3).

In the present invention, the sperm extract is characterized by exhibiting an anti-wrinkle effect by inhibiting MMP-1 (Matr ix metal loproteinase-1).

According to the experimental example of the present invention, it was confirmed that the sperm extract of the present invention inhibits MMP-1 increased by UVB. From these results, it can be seen that the sperm extract of the present invention can improve wrinkles by inhibiting the degradation of skin proteins such as collagen by inhibiting MMP-1 (see Experimental Example 1).

In the present invention, the sperm extract is characterized by exhibiting skin aging or wrinkle improvement effect by increasing the expression of procol lagen type I. According to the experimental example of the present invention, the content of procol lagen type I in the group irradiated with UVB on the artificial culture skin was decreased, whereas the content of procol lagen type I in the group treated with the extract of the present invention on the artificial culture skin irradiated with UVB. This increase was confirmed. From these results, it can be seen that the sperm extract of the present invention can improve skin aging or wrinkles by increasing the expression of procol lagen type I (see Experimental Example 4).

In the present invention, the composition is a skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisturizing cream, hand cream, foundation, essence, nutrition essence, pack At least one formulation selected from the group consisting of soaps, cleansing products, cleansing lotions, cleansing creams, body lotions and body cleansers.

Advantageous Effects

As described above, the dongjaja extract of the present invention as an active ingredient Containing cosmetic composition exhibits excellent effects in relieving stress in the skin and improving aging and wrinkles by effectively suppressing the expression of Ιββ-HSDl and MMP overexpressed by ultraviolet rays.

【Brief Description of Drawings】

1 is a view showing the results of experiments confirming the MMP-1 inhibitory activity of Donggyuja extract.

Figure 2 is a view showing the results of experiments confirming the inhibitory ability of ΙΙβ -HSDl of sperm extract.

Figure 3 is a diagram showing the results of experiments confirming the MMP-1 inhibitory ability of Donggyuja extract in artificial culture skin.

Figure 4 is a view showing the experimental results confirming the procol lagen type I increase ability of Donggyuja extract in artificial culture skin.

[Form for implementation of invention]

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only intended to illustrate the invention, and the scope of the invention is not to be construed as limited by these examples. Example 1 Preparation of Copper Enamel Extract

Donggyu used in the present invention was used to buy from the ^ 의 ^ Oriental medicine of the Republic of Korea.

100 g of copper ruler was taken and placed in a separate container. 70 wt% ethane was immersed in 1 L of an aqueous solution as an extraction solvent and left at room temperature for 72 hours to obtain an extract. The obtained extract was filtered using filter paper, and the filtrate was concentrated under reduced pressure with a rotary evaporator to obtain an extract of 10.0%. The obtained extract mixture was dissolved in DMS0 (dimethyl sul foxide) at a concentration of 1 mg / ml and 10 mg / ml as a solubilizer, and used in the following experimental example. Experimental Example 1 Evaluation of MMP-1 Inhibitory Activity of Copper Fructus Extract

In order to evaluate the MMP-1 inhibitory effect of the sperm extract of Example 1, the expression level of MMP-1 in human fibroblast cell line (Human dermal f ibroblast, Hs68) was measured.

Human fibroblasts were dispensed into 6wel l plates by the number of 4X10, and then cultured for 24 hours in an incubator at 37 ^° C, 5% C0 ₂ conditions. After removing the medium and putting DPBS, irradiated with 15mJ / cm ² of UVB to the remaining cell group except the UVB non-irradiated group was added to each concentration of incubation culture and further incubated for 24 hours. Then, RA was isolated from the cells of each sample by using trizol (RNA iso, DAKARA, Japan), quantified RNA at 260 nm using nanodrop, and then synthesized cDNA in an amplifier using each ig RNA. (C1000 Thermal Cycler, Bio-Rad, USA). The target protein in the synthesized cDNA MMP-1 template (primer) and a cyanine dye, SYBR Green (SYBR Green superrais, Appl ied Biosystems , USA) using a common compound added chain real-time polymerase in real ^¬ t ime PCR machine Finally, the expression level of the MMP-1 gene was evaluated. The sequence and reaction conditions of the template were as shown in Table 1 below, and the expression level of the gene was finally analyzed through correction for the β-act in gene. The results are shown in FIG. 1.

Table 1

As a result, as shown in FIG. 1, MMP-1 expression was increased in the UVB irradiation group, whereas MMP-1 gene expression was decreased in a concentration-dependent manner in the sperm extract treatment group. Experimental Example 2: Evaluation of ΙΙ β -HSDl Inhibitory Activity of Copper Palatinum Extract In order to evaluate the ΙΙβ -HSDl inhibitory effect of the sperm extract of Example 1, the experiment was conducted under the same conditions using the same cells as in Experimental Example 1. Finally, in the process of real-time polymerase chain reaction, Ιβ-HSDl template was used instead of MMP-1 template. The sequence and reaction conditions of the template were as shown in Table 1 above, and the expression level of the gene was finally analyzed through correction for β-act in gene. The results are shown in FIG. 2.

As a result, as shown in Fig. 2, it was confirmed that the expression level of ΙΙβ -HSDl gene was decreased in concentration-dependent manner in the sperm extract treatment group as in the MMP-1 gene expression amount. Experimental Example 3: Evaluation of MMP-1 Inhibitory Activity of Copper Palatinum Extract in Artificial Culture Skin

In order to evaluate the MMP-1 inhibitory effect of the sperm extract of Example 1 at the tissue level, an experiment was performed using EpiDermFT ^™ (MatTek, USA), which is a three-dimensional artificial cultured skin tissue.

EpiDermFT ™ containing both epidermis and dermis was first stabilized in the culture medium for 24 hours, and then the medium was removed, and 60 mJ / cm ² of UVB was irradiated to the remaining experimental groups except for the non-UVB irradiated group, and sperm extract was added by concentration Alternate with one medium and incubate further for 48 hours. Then, the culture medium obtained by culturing artificial skin tissue was collected and used to measure the amount of protein contained in the medium by removing suspended solids by centrifugation. The amount of MMP-1 protein contained in the culture medium was measured by the guided method using MMP-1 ELISA Ki t (EMD Mill ipore, USA).

As a result, as shown in FIG. 3, the MMP-1 content in the culture medium of the UVB irradiated group was higher than that of the unirradiated group, whereas the MMP-1 content was decreased in the same group as the unirradiated group. . Experimental Example 4: Procol lagen type I could be increased by sperm extract in artificial culture skin. The experiment was carried out under the conditions. Finally, the procol lagen type I content in the culture medium was measured by a guided method using Procol lagen type I C-peptide (PIP) EIA kit (Takara-bio Inc., Japan).

As a result, as shown in FIG. 4, the content of procol lagen type I in the culture medium of the UVB irradiated group was lower than that of the non-irradiated group. Formulation Example 1 Preparation of Toner

The toner agent containing the extract obtained in Example 1 was prepared in the usual manner according to the composition components and composition ratios shown in Table 2 below.

[Table 2]

Formulation Example 2: Preparation of Lotion

The lotion containing the extract obtained in Example 1 below

It was prepared by the usual method according to the composition components and composition ratios shown.

Table 3

Ingredient Content (% by weight)

Example 1 1.0

Glycerin 7.0

Burylene Glycol 3.0

Glyceryl Stearate / Fiji -100 Stearate 1.0

Plysorbate 60 0.5

Sorbitan Sesquioleate 0.5

Squalane 3.0

Caprylic / Capric Triglycerides 5.0 Shea Butter 2.0

Stearyl Alcohol 0.5

Stearic Acid 0.5

Carboxyl Vinyl Puller 0.2

Arginine 0.2

Preservative trace fragrance trace Purified water residual amount Formulation example 3: Preparation of cream

Cream containing the extract obtained in Example 1 was prepared according to the conventional method according to the composition and composition ratio shown below.

Table 4

Reference

(Patent Document 0001) KR 10-0825256 B

(Patent Document 0002) KR 10-1393007 B

Claims

[Range of request]

[Claim 1]

A cosmetic composition for improving skin aging or wrinkles, which contains an extract of Donggyuja as an active ingredient.

[Claim 2]

The method of claim 1, wherein the sperm extract is water, anhydrous or hydrous methane, at least one solvent selected from the group consisting of ethanol, propyl alcohol, butyl alcohol, glycerin, propylene glycol, butylene glycol, or a mixed solvent thereof Cosmetic composition for improving skin aging or wrinkles, characterized in that extracted.

[Claim 3]

The cosmetic composition for aging or wrinkle improvement according to claim 1, wherein the sperm extract comprises 0.01 to 50% by weight, based on the total weight of the composition.

[Claim 4]

The method of claim 1, wherein the extract is a cosmetic composition for improving skin aging or wrinkles by inhibiting llp -HSDl (ll) 3 -hydroxysteroid dehydrogenase type 1).

[Claim 5]

The composition of claim 1, wherein the extract of Donggyuja exhibits an effect of improving seed wrinkles by inhibiting MMP-1 (Matr ix metal loproteinase-1).

[Claim 6]

According to claim 1, wherein the sperm extract extracts the expression of procol lagen type I Skin aging or wrinkle improvement cosmetic composition, characterized in that by increasing the skin aging or wrinkle improvement effect.

[Claim 7]

According to claim 1, wherein the composition is a skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisturizing cream, hand cream, foundation, essence, nutrition essence, Cosmetic composition for skin aging or wrinkle improvement, characterized in that at least one formulation selected from the pack, soap, cleansing products, cleansing lotion, cleansing cream, body lotion and body cleanser.