JP6589244B2 - Skin preparation - Google Patents

Description

  The present invention relates to cosmetics such as lotion, emulsion, lotion, cream, gel, essence, lip balm, pack, hair tonic and hair cream, quasi-drugs and skin for external use of skin such as medicinal cosmetics and scalp preparations. For skin external preparations containing a base for external preparations, more specifically, skin external preparations with excellent moisturizing and roughening effects on skin that contain nicotinamide mononucleotide and yeast-fermented broth, and low irritation Regarding fees.

  In the modern living environment, skin is exposed to a dry environment throughout the year due to the effects of heating in the winter and cooling in the summer, and the rough skin becomes chronic. The fact that rough skin such as hands, arms, faces, bodies, legs and legs is a serious cosmetic concern is notable for the increase in the number of cosmetic products for men as well as for women. In particular, the face is a major factor in determining the first impression, so there are many people who want to have beautiful and moisturized young skin or white skin regardless of age or gender, and improve and prevent rough skin. There is a growing interest in skin external charges.

  Conventionally, in cosmetics, moisturizing effects such as polyglycols such as glycerin, saccharides, hyaluronic acid, pyrrolidone carboxylate, loofah water, yeast culture fluid, yeast extract, etc. are added to moisturize the skin. Many attempts have been made to make it high and glossy.

  In addition, cosmetics containing nicotinic acid mononucleotides, monosaccharides and oligosaccharides, and water-soluble film-forming polymer compounds for preventing or improving rough skin are known (Patent Document 1). Furthermore, nucleoprotein which prevents rough skin, spots, freckles and wrinkles and has skin whitening effect, and basic cosmetics containing DNA, RNA hydrolyzate deoxymononucleotide and oligonucleotide are known (Patent Document 2). .

  Furthermore, it has been studied to add nicotinic acid, nicotinic acid amide, and nicotinic acid mononucleotide to cosmetics in order to prevent or improve rough skin (Patent Documents 3 to 6).

  Furthermore, recently, in order to improve rough skin, it is known to add lactic acid bacteria and yeast culture solution containing nicotinic acid and nicotinamide (Patent Document 7).

  The causes of rough skin, dry skin, and various skin diseases are various factors such as decreased skin turnover and aging with aging, poor blood circulation, stress, ultraviolet rays, temperature, weather conditions, and other external factors. It is a direct cause that the metabolism of the cells decreases and the regenerative function of new cells falls. Conventionally known cosmetics for improving rough skin are a moisturizing retention effect on the surface of the skin and replenishment of oil, and sufficient effects are not obtained. In addition, nicotinic acid, nicotinamide, and nicotinic acid mononucleide, which are used to prevent and improve rough skin, and promote the synthesis of keratinocyte lipids, are not suitable for sensitive skin or atopic dermatitis. In some cases, the skin may be irritated and cannot be blended in cosmetics for such skin and scalp hair cosmetics, or can be blended only at low concentrations.

  The present inventors have found that nicotinamide mononucleotide is excellent in preventing and improving rough skin, and has little irritation to the skin, and prepared a cosmetic (Patent Document 8).

  However, nicotinamide mononucleotide has an excellent effect of preventing and improving rough skin, and also has a feature of less irritation to the skin, but there is a drawback that a satisfactory moisturizing effect cannot be obtained by its single formulation. .

  In order to provide a cosmetic having an excellent skin roughening action, it is known to add a fermented soymilk, nicotinic acid, nicotinic acid amide, benzyl nicotinate, a yeast culture, and the like (Patent Document 9).

  In order to reduce the irritation of lactic acid and enhance the moisturizing action, a cosmetic containing nicotinamide and a yeast culture solution is known (Patent Document 10).

  It is known that charcoal powder, nicotinic acid or nicotinic acid amide and a yeast culture solution are blended in order to provide cosmetics such as cleaning agents and packs that have an excellent moisturizing effect (Patent Document 11).

  In order to provide a skin cosmetic that can enhance the moisturizing action, it is known to combine carnitine, nicotinamide, and a yeast culture solution (Patent Document 12).

  As described above, it is known that a yeast culture solution and nicotinic acid or nicotinamide and carnitine are mixed to obtain cosmetics, cleaning materials, packs and the like having an excellent moisturizing effect. There is no known effect of improving the moisture retention of cosmetics or skin preparations containing mononucleotides.

Japanese Patent Laid-Open No. 2006-22051 JP 2007-291062 A JP 2010-174049 A JP 2007-45712 A JP 2008-150294 A JP 2010-1000066 A JP 09-194383 A JP-A-2006-135750 JP 2006-22050 A JP 2004-275550 A JP 2004-155684 A JP 2004-339140 A US Patent No. 4411995

New developments in the NAD world: Rhythm and aging of metabolism and brain function controlled by Sirt1 and systemic NAD synthesis system, Saitama Medical University Journal, No. 36, No. 2, pp. 131-132, 2010

  As a result of intensive studies, the inventors of the present invention have found that when nicotinamide mononucleotide and a culture solution fermented with yeast are used in combination, the moisturizing effect on the skin is greatly enhanced, and the effect of preventing and improving rough skin is excellent. It was found that there is little irritation to the skin.

  The object of the present invention is to provide cosmetics such as lotion, emulsion, lotion, cream, gel, essence, lip balm, pack, hair tonic, hair cream, etc. and quasi-drugs for external use on the skin such as medicinal cosmetics and scalp preparations. Alternatively, we provide a culture solution fermented with nicotinamide mononucleotide and yeast that has excellent moisturizing effect, prevention of skin roughening, and improvement effect that can be used in skin external preparations containing a base for external preparations for skin, and that is less irritating to the skin. The purpose is to do.

  The gist of the present invention relates to a skin external preparation excellent in a moisturizing effect and skin rough prevention and improvement effect, and having little irritation to the skin, and further contains a lotion containing a culture solution fermented with nicotinamide mononucleotide and yeast. A quasi-drug or base for external use for skin, such as milk, lotion, cream, gel, essence, lip balm, pack, hair tonic, hair cream, etc. It relates to a skin external preparation.

  The invention according to claim 1 relates to a skin external preparation characterized by blending a culture solution fermented with nicotinamide mononucleotide and yeast.

  The invention according to claim 2 relates to the external preparation for skin according to claim 1, wherein the yeast contains one or more of the genus Saccharomyces, the genus Galactomyces or the genus Endomyces.

  According to the invention of claim 1, a culture solution fermented with nicotinamide mononucleotide and yeast having a moisturizing effect on skin, which is excellent in preventing and improving rough skin and having little irritation to the skin By the synergistic action consisting of the above, it is possible to provide a skin external preparation which is excellent in the moisturizing effect and the prevention and improvement effect of rough skin, and has little irritation to the skin.

  According to the invention according to claim 2, the moisturizing effect and skin roughening prevention and improvement effect due to the synergistic effect of the culture solution fermented with yeast containing one or more of the genus Saccharomyces, Galactomyces or Endomyces and nicotinamide mononucleotide. Cosmetics and medicinal cosmetics selected from the group consisting of skin lotions, emulsions, lotions, creams, gels, essences, lip balms, packs, hair tonics, hair creams, which are excellent and less irritating to the skin, and It is possible to provide a skin external preparation containing a quasi-drug or a skin external preparation base for external use on the skin such as a scalp preparation.

The nicotinamide mononucleotide used in the present invention is a nucleotide in which ribose 5-phosphate is β-linked to the N1 position of nicotinamide, and is an intermediate metabolite of nicotinamide and NAD. In addition to being obtained from NAD by enzymatic degradation with nucleotide pyrophosphatase, it can be prepared from adenosine monophosphate or ribose-5-phosphate sodium by a chemical synthesis method (Patent Document 13). In addition, an enzyme synthesis method for producing nicotinamide mononucleotide from nicotinamide by nicotinamide phosphoribosyl transferase (Nampt) is also known (Non-patent Document 1).
The nicotinamide mononucleotide used in the present invention is an alkali metal salt such as lithium, sodium and potassium, an alkaline earth metal salt such as magnesium and calcium, and an ammonium such as ammonium and tetramethylammonium, as long as the effects of the present invention are not impaired. It can also be used as an organic amine salt such as a salt, triethylamine, piperidine, morpholine and toluidine, or an amino acid addition salt such as lysine, arginine and histidine.

  The nicotinamide mononucleotide used in the present invention is a cosmetic or medicinal cosmetic and scalp selected from the group consisting of the lotion, emulsion, lotion, cream, gel, essence, lip balm, pack, hair tonic and hair cream of the present invention. 0.001 to 10.0% by weight, more preferably 0.01 to 7.0% by weight, in a skin external preparation containing a quasi-drug for external use on the skin such as a preparation, or a base for external preparations for skin To do. If the amount is less than 0.001% by weight, the rough skin prevention, improvement effect and whitening effect of the present invention cannot be obtained. If the amount exceeds 10.0% by weight, the increase in the rough skin prevention, improvement effect and whitening effect cannot be expected. The stimulus is large and not economically appropriate.

  The culture solution fermented with the yeast used in the present invention is a known one and is used as a skin external preparation. The yeast used in the present invention may be of any kind as long as it achieves the purpose of the present invention, but yeast belonging to the genus Saccharomyces, the genus Galactomyces or the genus Endomyces can be used. More specifically, Saccharomyces cerevisiae, Galactomyces candidus, Endomyces magnusii can be used. As the culture medium, synthetic media containing carbon sources and nitrogen sources, milk, skim milk aqueous solution, whey, soy milk, and aqueous solutions containing partial degradation products of plants such as rice, rice bran, corn, and wheat are used. Can be used. Usually, a culture solution obtained by fermenting yeast in such a medium is one in which the fermentation culture product is left or filtered to remove solids and sterilized with a membrane filter or the like, or the sterilized one is concentrated under reduced pressure. "Yeast Fermented Extract" (INCI name Yeast Ferment Extract) and "Galactomyces broth" INCI published in "Cosmetic Ingredient Name List" published by Japan Cosmetic Industry Association It is represented by the name Galactomics Ferment Filrate), “Yeast Culture Solution” INCI name Endomyces Ferment Filtrate), and is commercially available as trade names: B, B Essence (Vesuvius).

  The culture solution fermented with yeast used in the present invention is a cosmetic or medicinal product selected from the group consisting of the lotion, emulsion, lotion, cream, gel, essence, lip balm, pack, hair tonic and hair cream of the present invention. 0.01 to 95.0% by weight, more preferably 1.0 to 90.0% for skin external preparations including bases for quasi-drugs or external preparations for external use on the skin such as cosmetics and scalp preparations. Mix by weight. If the amount is less than 0.01% by weight, the moisturizing effect of the present invention cannot be obtained. If the amount exceeds 95.0% by weight, an increase in the moisturizing effect cannot be expected, and the storage stability is not preferred. Not.

  In addition to the culture solution fermented with the nicotinamide mononucleotide of the present invention and yeast, the cosmetics of the present invention are usually polyhydric alcohols such as glycerin and 1,3 butylene glycol used in cosmetics and quasi drugs. , Moisturizers such as sodium pyridodone carboxylate, sodium hyaluronate, citric acid, oil component, surfactant, dispersion stabilizer, thickener, wetting agent, physiologically active ingredient, blood circulation promoter, whitening agent, vitamins , Amino acids, enzymes, dyes, pigments, fragrances, preservatives, antioxidants, UV absorbers, chelating agents, pH adjusters, purified water, alcohols, etc., combined within the scope of achieving the object of the present invention. Can do.

  The cosmetic of the present invention can be produced according to a conventional method. The cosmetics of the present invention include skin lotions, emulsions, lotions, creams, gels, essences, lip balms, packs, hair tonics, hair creams, and other external skin applications such as cosmetics, medicinal cosmetics, and scalp preparations. Quasi-drugs or skin external preparations containing a base for external preparations for skin, etc., and the dosage form is arbitrarily selected according to the purpose, such as liquid, cream, gel, emulsion, sheet, aerosol You can choose. The external preparation for skin of the present invention is not limited to general cosmetics and medicinal cosmetics, but includes quasi-drugs, external medicines and bases thereof.

  Next, although an Example demonstrates this invention concretely, this invention is not limited only to the said Example.

Example 1
An example of a cosmetic formulation containing the nicotinamide mononucleotide of the present invention (manufactured by Sigma-Aldrich) and a culture solution fermented with yeast: yeast culture solution (trade names: B, B essence, manufactured by Vesuvius) is shown. In addition, a compounding quantity is weight%.
(Formulation 1: lotion)
Yeast culture solution 90.0
Nicotinamide mononucleotide 2.0
Glycerin 3.0
1,3 butylene glycol 6.0
Hydroxyethyl cellulose 0.02
Preservative appropriate amount Purified water Total 100
(Formulation 2: lotion)
Yeast culture solution 70.0
Nicotinamide mononucleotide 1.0
Glycerin 3.0
Propylene glycol 2.0
Hydroxyethyl cellulose 0.02
Ethanol 10.0
Perfume and preservatives Suitable amount Purified water Total 100
(Formulation 3: lotion)
Yeast culture solution 30.0
Nicotinamide mononucleotide 0.005
Glycerin 3.0
Propylene glycol 2.0
Oleyl alcohol 0.1
Polyoxyethylene (20) sorbitan monolaurate 1.5
Ethanol 10.0
Perfume and preservatives Suitable amount Purified water Total 100
(Formulation 4: Latex)
Liquid paraffin 10.0
Stearic acid 0.2
Cetanol 1.5
Vaseline 3.0
Lanolin alcohol 2.0
Polyoxyethylene (10) monooleate 2.0
Glycerin 3.0
Propylene glycol 5.0
Triethanolamine 1.0
Nicotinamide mononucleotide 1.0
Yeast culture solution 10.0
Perfume and preservatives Suitable amount Purified water Total 100
(Formulation 5: Lotion)
Glycerin 5.0
1.3-Butylene glycol 2.0
Propylene glycol 2.0
Oleyl alcohol 0.1
Polyoxyethylene (20) sorbitan monolaurate 0.5
Ethanol 10.0
Nicotinamide mononucleotide 0.5
Yeast culture solution 10.0
Perfume, preservatives, pH adjuster Suitable amount Purified water Total 100
(Formulation 6: Cream)
Squalane 5.0
Stearic acid 2.0
Stearyl alcohol 7.0
Reduced lanolin 2.0
Octyldodecanol 6.0
Polyoxyethylene (25) cetyl alcohol 3.0
Lipophilic glyceryl monostearate 2.0
Propylene glycol 5.0
Nicotinamide mononucleotide 1.0
Yeast culture 15.0
Perfume and preservatives Suitable amount Purified water Total 100
(Prescription 7: Cold cream)
Solid paraffin 5.0
Liquid paraffin 35.0
Vaseline 15.0
Beeswax 10.0
Jojoba oil 5.0
Lipophilic glyceryl monostearate 2.0
Polyoxyethylene (20) sorbitan monolaurate 1.5
Stearyl alcohol 5.0
Soap powder 0.1
Nicotinamide mononucleotide 0.5
Yeast culture solution 10.0
Fragrance / preservative 0.1
Purified water Total 100
(Prescription 8: Gel)
Polyalkyl acrylate powder 5.0
Ethanol 8.0
Carboxyvinyl polymer 0.2
Nicotinamide mononucleotide 5.0
Yeast culture solution 0.05
Potassium hydroxide 0.02
Perfume and preservatives Suitable amount Purified water Total 100
(Prescription 9: Essence)
Glycerin 5.0
1,3-butylene glycol 2.0
Sodium hyaluronate 0.01
Xylitol 0.5
Seaweed extract 0.1
Nicotinamide mononucleotide 10.0
Yeast culture solution 20.0
Fragrance / preservative, pH adjuster Suitable amount Purified water Total 100
(Formulation 10: Lip Balm)
Solid paraffin 10.4
Castor oil 20.0
Lanolin 15.0
Beeswax 5.0
Cadilla Laro 10.0
Carnavalou 5.0
Cetyl 2-ethylhexanoate 20.0
Isopropyl myristate 13.0
Nicotinamide mononucleotide 0.5
Yeast culture solution 5.0
Fragrance / preservative 0.1
(Prescription 11: Pack)
Polyvinyl alcohol 15.0
Sodium carboxymethylcellulose 5.0
Propylene glycol 3.0
Ethanol 10.0
Nicotinamide mononucleotide 0.1
Yeast culture solution 30.0
Perfume and preservatives Suitable amount Purified water Total 100
(Prescription 12: Hair Tonic)
DL-α-tocophenol acetate 0.1
Polyethylene glycol 300 0.1
Polyoxyethylene stearyl ether (20EO) 2.0
Isopropyl myristate 3.0
L-Menthol 0.2
Ethanol 80.0
Nicotinamide mononucleotide 0.1
Yeast culture solution 0.5
Perfume and preservatives Suitable amount Purified water Total 100
(Prescription 13: Hair cream)
DL-α-tocophenol acetate 0.1
Dimethyl silicone gum (degree of polymerization 12000) 1.0
Ethyl oleate 2.0
Isopropyl myristate 2.0
Liquid paraffin 1.0
Polyethylene glycol monostearate (40EO) 3.0
Lipophilic glyceryl monostearate 1.5
Stearyl alcohol 2.0
L-Menthol 0.3
Polyethylene glycol 1500 1.0
1,3-butylene glycol 2.0
Nicotinamide mononucleotide 1.0
Yeast culture solution 10.0
Perfume and preservatives Suitable amount Purified water Total 100
(Prescription 14: Hair lotion)
Glycerin 2.0
1,3-butylene glycol 2.0
Propylene glycol 2.0
Oleyl alcohol 0.1
Polyoxyethylene (20) sorbitan monolaurate 0.5
Ethanol 30.0
Nicotinamide mononucleotide 0.7
Yeast culture solution 5.0
Perfume and preservatives Suitable amount Purified water Total 100

Comparative Example 1
(Comparison formula 1: lotion)
Glycerin 3.0
1,3 butylene glycol 6.0
Hydroxyethyl cellulose 0.02
Preservative appropriate amount Purified water Total 100
Comparative Example 2
(Comparison formula 2: lotion)
Nicotinamide mononucleotide 2.0
Glycerin 3.0
1,3 butylene glycol 6.0
Hydroxyethyl cellulose 0.02
Preservative appropriate amount Purified water Total 100
Comparative Example 3
(Comparison prescription 3: lotion)
Glycerin 3.0
Propylene glycol 2.0
Oleyl alcohol 0.1
Polyoxyethylene (20) sorbitan monolaurate 1.5
Yeast culture solution 3.0
Ethanol 20.0
Perfume and preservatives Suitable amount Purified water Total 100

Comparative Example 4
Nicotinamide mononucleotide of the present invention (manufactured by Sigma-Aldrich) 0.0009% by weight and culture solution fermented with yeast: yeast culture solution (trade name: B, B essence, manufactured by Vesuvius) 0.009% by weight The comparative prescription example of an emulsion is shown as cosmetics to contain. In addition, a compounding quantity is weight%.
(Comparison prescription 4: emulsion)
Liquid paraffin 10.0
Stearic acid 0.2
Cetanol 1.5
Vaseline 3.0
Lanolin alcohol 2.0
Polyoxyethylene (10) monooleate 2.0
Glycerin 3.0
Propylene glycol 5.0
Triethanolamine 1.0
Nicotinamide mononucleotide 0.0009
Yeast culture solution 0.009
Perfume and preservatives Suitable amount Purified water Total 100

Test example 1 Moisturizing effect (moisturation feeling) improvement test, rough skin improvement test and irritation evaluation test The test of the prescription 1 of Example 1 was conducted using 10 middle-aged female subjects whose skin of the lower limbs easily dried and suffered from rough skin as panelists. To the lower limb of the skin lotion, the culture solution fermented with yeast from the skin lotion of the formulation 1 of Example 1: The skin lotion of the formulation excluding the yeast culture fluid and nicotinamide mononucleotide (Comparative Example 1) It was applied to the lower limb once a day after bathing. According to the following criteria, the panelists themselves evaluated and obtained a response according to the skin condition of the place where the test was applied for 1 month before the test was conducted. The results are shown in Table 1.
Criteria for evaluating the moisturizing effect of the skin as a moist feeling +++: Moisture feeling is greatly improved compared to before use (effectively large)
++: Moisture feeling improved considerably before use (effective)
+: Slightly improved moist feeling compared to before use (somewhat effective)
−: Moist feeling is less than or equal to that before use (invalid)
Judgment criteria for skin smoothness, fine texture, and skin elasticity +++: Significantly improved compared to before use (effectively large)
++: Significant improvement compared to before use (effective)
+: Slightly improved compared to before use (somewhat effective)
−: Less than or equal to that before use (invalid)
Criteria for determining skin irritation +++: Feeling considerable irritation (Large irritation)
++: I feel a sense of stimulation (Stimulation)
+: Slight irritation (slight irritation)
−: No sense of irritation (no irritation)

  As can be seen from the test results in Table 1, the skin lotion containing the product of the present invention clearly has a skin moisturizing improvement effect and skin improvement effect than the comparative skin lotion, and there is no skin irritation. I understand that.

Test Example 2 Moisturizing effect (moisturation) improvement test, rough skin improvement test and irritation evaluation test As in Test Example 1, the panel of 10 middle-aged female subjects suffering from rough skin, where the skin of the lower limbs is easy to dry. The emulsion of the formulation 3 of Example 1 was applied to one lower limb and the emulsion of the comparative formulation of Comparative Example 4 was applied to the other lower limb once a day after bathing. According to the following criteria, the panelists themselves evaluated and obtained a response according to the skin condition of the place where the test was applied for 1 month before the test was conducted. The results are shown in Table 2.
Criteria for evaluating the moisturizing effect of the skin as a moist feeling +++: Moisture feeling is greatly improved compared to before use (effectively large)
++: Moisture feeling improved considerably before use (effective)
+: Slightly improved moist feeling compared to before use (somewhat effective)
−: Moist feeling is less than or equal to that before use (invalid)
Judgment criteria for skin smoothness, fine texture, and skin elasticity +++: Significantly improved compared to before use (effectively large)
++: Significant improvement compared to before use (effective)
+: Slightly improved compared to before use (somewhat effective)
−: Less than or equal to that before use (invalid)
Criteria for determining skin irritation +++: Feeling considerable irritation (Large irritation)
++: I feel a sense of stimulation (Stimulation)
+: Slight irritation (slight irritation)
−: No sense of irritation (no irritation)

  As can be seen from the test results in Table 2, the emulsion containing the product of the present invention clearly has a moisturizing improvement effect on the skin and an improvement effect on the skin and no skin irritation feeling than the comparison emulsion. .

  The skin external preparation containing the nicotinamide mononucleotide of the present invention and a culture broth fermented with yeast (yeast culture fluid) has a synergistic effect to keep the skin moisturized, smooth and smooth, smooth skin. It is clear that it has the effect of improving the moisture retention and rough skin and less irritation from the improvement of skin properties. The external preparation for skin containing the nicotinamide mononucleotide of the present invention and a culture broth fermented with yeast (yeast culture fluid) is a decrease in skin turnover with aging, aging, poor circulation, stress, ultraviolet light and temperature. In order to improve the deterioration of skin cell metabolism due to external factors such as weather conditions, and the deterioration of the renewal function of new cells, the skin is moistened and smoothed with rough skin, dry skin and wrinkled skin It is considered to be a beautiful skin with a beautiful elasticity and gloss. Furthermore, the nicotinamide mononucleotide to be blended in the present invention has a nicotinamide group in its molecular structure, so it is considered to have a whitening action and can be blended into a whitening cosmetic. Furthermore, the blood circulation promoting action and the activation and regeneration of hair cells are expected, and it can be added to a hair nourishing agent or a hair restoration agent.

  The culture solution fermented with nicotinamide mononucleotide of the present invention and yeast (yeast culture solution) is selected from the group consisting of lotion, emulsion, lotion, cream, gel, essence, lip balm, pack, hair tonic and hair cream. It can be used as a quasi-drug for external use on the skin such as cosmetics, medicinal cosmetics, and scalp preparations, or skin external preparations containing a base for external preparations for skin.

Claims (2)

  An external preparation for skin, comprising (a) nicotinamide mononucleotide and (b) a culture solution fermented with yeast.   The external preparation for skin according to claim 1, wherein the yeast of (a) includes one or more of the genus Saccharomyces, the genus Galactomyces or the genus Endomyces.