KR20220124390A - Cosmetic composition for preventing or improving hair loss symptoms containing black soybean, green tangerine and turmeric extract - Google Patents

Abstract

The present invention relates to a composition including a Glycine max seed extract, a Citrus reticulata fruit extract, and a Curcuma longa root extract. The composition including a Glycine max seed extract, a Citrus reticulata fruit extract, and a Curcuma longa root extract according to the present invention is excellent in preventing or alleviating hair loss.

Description

Cosmetic composition for preventing or improving hair loss symptoms containing black beans, green tangerine and turmeric extracts {Cosmetic composition for preventing or improving hair loss symptoms containing black soybean, green tangerine and turmeric extract}

The present invention relates to a cosmetic composition for preventing or improving hair loss symptoms comprising extracts of black beans, green tangerines and turmeric.

Hair is responsible for various roles in the human body, such as protecting the head, affecting the appearance, and maintaining the temperature of the head.

The human body has about 100,000 to 150,000 hairs, and each hair has a different cycle and accounts for 90% of normal hair in the anagen (growing phase), growth stop and catagen (transitional phase), in which the hair roots are atrophied. Hair goes through a hair cycle, such as a telogen, resting phase, in which the hair follicle dries and becomes a club hair, and hair grows and loses hair repeatedly. This cycle is repeated over 3 to 6 years, and as a result, an average of 50 to 100 hairs are normally lost per day.

Alopecia refers to a phenomenon in which the proportion of hair in the growth phase is shortened in the hair growth cycle and the hair in the degenerative phase or telogen phase increases, abnormally increased hair loss, and the normally grown hair falls out due to some cause. Most hair loss, other than hair loss due to stress or disease, causes the hair to lose nutrients and naturally become thinner and weaker and fall out. Hair loss is known to occur due to unbalanced diet, excessive drinking and smoking, drug use, lack of sleep, stress, childbirth, environmental pollution, heredity, frequent perms and dyeing, and hormone imbalance.

Hair loss treatment methods include topical application agents, oral medications, health supplements, hair transplant surgery, etc. However, these methods have a limited scope of use, and continuous hair loss relief is not achieved. In addition, the effect of the drug treatment is temporary, and rather accelerates the production of the scalp or sebum.

To treat hair loss, the approach is to increase hair follicle cells in the growth phase (anagen) and decrease cells in the catagen phase and telogen phase. It activates Wnt/β-catenin, which is decreased in the process of hair loss, and differentiates cells involved in hair formation by increasing IGF-1 and VEGF, which are hair growth factors.

Minoxidil (transdermal application) and finasteride (oral preparation), which are currently the most well-known hair growth stimulants, have been approved by the FDA and are being used clinically. In particular, minoxidil was first introduced as a vasodilator in the early 1970s, and it was found that side effects other than drug effects were hair growth, so it is currently used as a hair growth promoting agent. is being used However, there are significant limitations in active treatment as adverse drug reactions have been reported for the currently used hair growth agents.

In consideration of these problems, there is a need for the development of natural raw materials that are safe for consumers to trust and use, and have excellent efficacy in alleviating hair loss and promoting hair growth.

Registered Patent Publication No. 10-1424175

It is an object of the present invention to provide a cosmetic composition for preventing or improving hair loss.

Another object of the present invention is to provide a composition for external application for skin for preventing or improving hair loss symptoms.

In order to achieve the above object,

The present invention provides a cosmetic composition for preventing or improving hair loss symptoms, including a black bean ( Glycine max seed) extract, a Citrus reticulata fruit extract, and a Curcuma longa root extract.

The black soybean may use a black soybean raw material, preferably black soybean oil oil (defatted Glycine max seed) may be used as an extraction target.

In addition, the present invention provides a composition for external application for skin for preventing or improving hair loss symptoms, comprising a black bean ( Glycine max seed) extract, a green tangerine ( Citrus reticulata fruit) extract, and a turmeric ( Curcuma longa root) extract.

The black soybean may use a black soybean raw material, preferably black soybean oil oil (defatted Glycine max seed) may be used as an extraction target.

A composition comprising a black bean ( Glycine max seed) extract, a Citrus reticulata fruit extract and a Curcuma longa root extract according to the present invention has an excellent effect in preventing or improving hair loss.

1 is a flow chart showing the manufacturing steps of black soybean extract.
2 is a flowchart showing the steps of preparing a green tangerine extract.
Figure 3 is a flow chart showing the manufacturing steps of turmeric extract.

Hereinafter, the present invention will be described in detail.

cosmetic composition

The present invention provides a cosmetic composition comprising an active substance.

The cosmetic composition is, for example, a solution, a gel, a solid or kneaded anhydrous product, an emulsion obtained by dispersing an oil phase in an aqueous phase, a suspension, a microemulsion, a microcapsule, a microgranule or an ionic (liposome), a nonionic vesicle dispersant may be in the form of More specific forms include lotions, emulsions, creams, skins, lotions, serums, essences, emulsions, powders, cosmetic ointments, sprays, gels, packs, cleansers, soaps, shampoos, conditioners, bathing agents, detergents, or in the form of sticks. can be In addition, it may be prepared in the form of a foam or in the form of an aerosol composition further containing a compressed propellant.

In addition, the cosmetic composition comprises a fatty substance, an organic solvent, a solubilizer, a thickening agent and a gelling agent, an emollient, an antioxidant, a suspending agent, a stabilizer, a foaming agent, a fragrance, a surfactant, Commonly used in water, ionic or nonionic emulsifiers, fillers, sequestering and chelating agents, preservatives, vitamins, blocking agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic actives, lipid vesicles or cosmetics It may contain adjuvants commonly used in the cosmetic field, such as any other ingredients that are used.

In the cosmetic composition containing the active substance of the present invention, the active substance of the present invention may be added in an amount of 0.1 to 50% by weight, preferably 1 to 10% by weight, to the cosmetic composition usually contained.

When the active substance of the present invention is used as an external preparation for skin, additionally, a fatty substance, an organic solvent, a solubilizer, a thickening agent and a gelling agent, an emollient, an antioxidant, a suspending agent, a stabilizer, a foaming agent, a fragrance, a surfactant , water, ionic or non-ionic emulsifiers, fillers, sequestering and chelating agents, preservatives, vitamins, blocking agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or for external use on the skin It may contain adjuvants commonly used in the field of dermatology, such as any other ingredients used as a skin care product. In addition, the above ingredients may be introduced in an amount generally used in the field of dermatology.

Composition for external application for skin

The ingredients included in the composition of the present invention may include ingredients commonly used in external composition for skin in addition to the active ingredient, for example, conventional adjuvants such as antioxidants, stabilizers, solubilizers, vitamins, pigments and fragrances, and carriers, excipients and/or diluents. Examples of suitable carriers, excipients and diluents for formulation include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, malditol, starch, acacia gum, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, Methylcellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate or mineral oil may be used.

When applied to pharmaceuticals, it can be formulated as a parenteral administration in the form of a solid, semi-solid or liquid by adding a commonly used inorganic or organic carrier.

In order to formulate the active ingredient of the present invention, it can be easily formulated according to a conventional method, and surfactants, excipients, colorants, spices, preservatives, stabilizers, buffers, suspending agents, and other compatibilizing adjuvants can be appropriately used.

The composition for external application for skin of the present invention may be prepared in any formulation conventionally prepared in the art, for example, may be formulated in the form of an ointment, patch, gel, cream or spray, but is not limited thereto. .

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the following examples only illustrate the present invention, and the content of the present invention is not limited by the following examples.

<Preparation Example 1> Black beans ( Glycine max seed) preparation of extract

Black soybean extract was prepared as in the preparation step of FIG. 1 . Black soybean ( Glycine max ) seeds are pressed and milked to obtain oilmeal. After mixing 1L of purified water with 50 g of the oil cake, 0.5 g of each of Protamex ^® (manufacturer: novozymes) and Flavorzyme ^® (manufacturer: novozymes) as a protease was mixed, and enzymatically hydrolyzed at 50° C. for 4 hours to obtain an extract. The extract was filtered with filter paper (Filter paper, Hyundai Micro, 1-2 μm). The filtered filtrate was first inactivated by heating at 90° C. for 5 minutes or more, and then dried with a freeze dryer to prepare a black soybean extract.

<Preparation Example 2> Green tangerine ( Citrus reticulata fruit) Preparation of extract

A green tangerine extract was prepared as in the preparation step of FIG. 2 . To 100 g of unripe Citrus reticulata fruit, 900 mL of distilled water was added as an extraction solvent, and extraction was performed at 90° C. for 3 hours in a shaking water bath (KMC-1205SW1, Vision, Daejeon, Korea) to obtain an extract. The extract was filtered with filter paper (Filter paper, Hyundai Micro, 1-2 μm). The filtered filtrate was dried using a freeze dryer to prepare a green tangerine extract.

<Preparation Example 3> Turmeric ( Curcuma longa root) preparation of extract

A turmeric extract was prepared as in the preparation step of FIG. 3 . To 100 g of turmeric ( Curcuma longa ) roots, 900 mL of distilled water was added as an extraction solvent, and extraction was performed at 90° C. for 3 hours in a shaking water bath (KMC-1205SW1, Vision, Daejeon, Korea) to obtain an extract. The extract was filtered with filter paper (Filter paper, Hyundai Micro, 1-2 μm). The filtered filtrate was dried using a freeze dryer to prepare a turmeric extract.

<Example 1> Black beans ( Glycine max seed) extract, green tangerine ( Citrus reticulata fruit) extract and turmeric ( Curcuma longa root) preparation of extract mixture

A mixture sample was prepared by mixing the three extracts prepared in Preparation Examples 1 to 3, butylene glycol, and purified water.

Specifically, a sample was prepared by mixing 0.1% by weight of black bean extract, 0.1% by weight of green tangerine extract, 0.1% by weight of turmeric extract, 40% by weight of butylene glycol and the remaining amount of purified water.

<Comparative Example 1> Preparation of black soybean extract sample

A sample was prepared by mixing 0.3% by weight of the black soybean extract prepared in Preparation Example 1, 40% by weight of butylene glycol, and the remaining amount of purified water.

<Comparative Example 2> Preparation of green tangerine extract sample

A sample was prepared by mixing 0.3% by weight of the green tangerine extract prepared in Preparation Example 2, 40% by weight of butylene glycol, and the remaining amount of purified water.

<Comparative Example 3> Preparation of turmeric extract sample

A sample was prepared by mixing 0.3% by weight of the turmeric extract prepared in Preparation Example 3, 40% by weight of butylene glycol, and the remaining amount of purified water.

<Comparative Example 4> Preparation of samples containing black soybean extract and green tangerine extract

A sample was prepared by mixing 0.15% by weight of the black soybean extract prepared in Preparation Example 1, 0.15% by weight of the green tangerine extract prepared in Preparation Example 2, 40% by weight of butylene glycol, and the remaining amount of purified water.

<Comparative Example 5> Preparation of samples containing black soybean extract and turmeric extract

A sample was prepared by mixing 0.15% by weight of the black bean extract prepared in Preparation Example 1, 0.15% by weight of the turmeric extract prepared in Preparation Example 3, 40% by weight of butylene glycol, and the remaining amount of purified water.

<Experimental Example 1> Human hair papilla cell cell viability evaluation

The samples prepared in Examples and Comparative Examples were treated with human hair papilla cells to evaluate cell viability, and hair loss efficacy was investigated.

High sensitivity water soluble tetrazolium salts (WST) react with dehydrogenase in the cell to generate formazan. Therefore, the more live cells there are, the more formazan is produced, which indicates a high absorbance, which can be used to determine the degree of toxicity of the cells.

Human Follicle Dermal Papilla Cell (Manufacturer: Promo cell) used in this test was prepared by adding Fetal Calf Serum, Bovine Pituitary Extract, Basic Fibroblast Growth Factor, and Insulin to Human Follicle Dermal Papilla Cell Growth Medium (Manufacturer: PromoCell). It was cultured in a medium at 37° C., 5% CO ₂ condition.

The cultured cells were divided into 5 X 10 ³ pieces in a 96-well plate, and when cultured to 80% or more, the samples were treated at 5 concentrations and then cultured for 24 or 48 hours. Then, a WST substrate solution (Model: CCK-8; Manufacturer: Dojindo) was added to the medium and incubated at 37°C for 2 hours.

The absorbance (OD) was measured at 450 nm using a microplate reader (VARIOSKAN LUX, Thermo Fisher Scientific), and the cell viability was calculated by the following equation.

Cell viability (%) = (OD _exp -OD _blnk )/(OD _con -OD _blnk ) X 100

- blank (blnk): medium containing WST substrate

- Control (con, negative control): untreated group

- Experiment(exp, experimental group): sample treatment group

Incubation time after sample treatment (h) Sample treatment concentration (ppm) 0 3.125 6.25 12.5 25 Example 1 (black bean + green tangerine + turmeric) 24 100.000 106.365 112.566 128.214 146.729 48 103.856 108.131 119.789 164.493 Comparative Example 1 (black soybean) 24 102.854 104.135 109.269 121.583 48 98.412 100.889 104.887 116.518 Comparative Example 2 (Green Tangerine) 24 101.544 102.178 104.216 109.326 48 100.654 101.524 102.558 104.213 Comparative Example 3 (turmeric) 24 101.958 103.569 106.312 112.258 48 100.998 102.007 103.441 107.481 Comparative Example 4 (black bean + green tangerine) 24 103.254 108.269 113.458 131.257 48 101.303 104.210 108.558 120.087 Comparative Example 5 (black soybean + turmeric) 24 103.026 109.007 118.859 133.251 48 101.227 105.334 115.589 128.887

As shown in Table 1, in the case of the three extract complex samples of Example 1 compared to Comparative Examples 1 to 5, it was confirmed that the survival rate of human hair papilla cells was significantly improved. In particular, Comparative Examples 1 to 5 all showed a decrease in cell viability after 48 hours compared to 24 hours after sample treatment at a treatment concentration of 25 ppm, whereas in Example 1, cell viability was higher after 24 hours compared to after 48 hours. appeared to improve.

<Experimental Example 2> Skin irritation evaluation

Example 1 The safety of the sample was evaluated by evaluating the skin irritation.

The sample of Example 1 was prepared in a 1% dilution state, and a 24-hour patch test was performed on 31 adults. A dermatologist judged the presence or absence of primary skin irritation at 30 minutes, 24 hours, and 48 hours after patch removal, respectively.

The skin irritation scoring system was as follows.

<Skin irritation score system table>

The irritation index was calculated as follows using the skin response scores of the study subjects.

After calculating the average irritation index, the Draize Dermal Classification System and EPA (Environmental Protection Agency) Standard Procedure Dermal Classification System are applied, and then the degree of irritation is determined according to the result judgment table.

stimulus index Irritation Assessment 0≤ <0.02 non-irritant 0.02≤ <0.25 hypoallergenic 0.25≤ <1 mild stimulation 1≤ <2.5 central stimulus 2.51≤ strong stimulus

Skin reaction determination was based on ICDRG (International contact dermatitis research group) standards and PCPC (Personal Care Products Council) guidelines. obtained, and it was confirmed that it was a hypoallergenic sample.

Preparation example of cosmetics

The active substance according to the present invention can be prepared into various types of cosmetics depending on the purpose. The following exemplifies the manufacturing method of several cosmetics containing the active ingredient according to the present invention as an active ingredient, but the present invention is not limited thereto.

<Cosmeceutical preparation example 1> Preparation of softened lotion

active substance 10.0 parts by weight

1,3-butylene glycol 1.00 parts by weight

Disodium EDIT 0.05 parts by weight

allantoin 0.10 parts by weight

Dipotassium glycyrrhizate 0.05 parts by weight

citric acid 0.01 parts by weight

sodium citrate 0.02 parts by weight

Glyceres-26 1.00 parts by weight

arbutin 2.00 parts by weight

Hydrogenated Castor Oil 1.00 parts by weight

ethanol 30.0 parts by weight

preservative a very small amount

coloring agent a very small amount

flavoring agent a very small amount

Purified water remaining amount

<Cosmeceutical Preparation Example 2> Preparation of nourishing cream

active substance 10.0 parts by weight

1,3-butylene glycol 7.00 parts by weight

glycerin 1.00 parts by weight

D-Panthenol 0.10 parts by weight

plant extract 3.20 parts by weight

Magnesium Aluminum Silicate 0.30 parts by weight

PEG-40 Stearate 1.20 parts by weight

stearic acid 2.00 parts by weight

Polysorbate 60 1.50 parts by weight

Lipophilic Glyceryl Stearate 2.00 parts by weight

Sorbitan sesquioleate 1.50 parts by weight

cetearyl alcohol 3.00 parts by weight

mineral oil 4.00 parts by weight

squalane 3.80 parts by weight

Carlylic/Capric Triglycerides 2.80 parts by weight

vegetable oil 1.80 parts by weight

dimethicone 0.40 parts by weight

Dipotassium glycyrrhizate a very small amount

allantoin a very small amount

Sodium Hyaluronate a very small amount

Tocopheryl Acetate appropriate amount

triethanolamine appropriate amount

preservative appropriate amount

flavoring agent appropriate amount

Purified water remaining amount

Preparation example of external preparation for skin

The active substance according to the present invention can be formulated in various forms depending on the purpose. The following exemplifies some formulation methods containing the active substance according to the present invention as an active ingredient, but the present invention is not limited thereto.

<Skin external preparation Preparation Example 1> Preparation of spray

active substance 100 mg

Lee Seonghwadang 10 g

mannitol 5 g

Purified water appropriate amount

According to a conventional method for producing a spray, add each component to purified water to dissolve, add lemon flavor, mix the above ingredients, add purified water to adjust the total to 100 mL, fill in a brown bottle, and sterilize to prepare a spray did.

<Production Example 2 for external application for skin> Preparation of gel

active substance 100 mg

ethanol 10 g

glycerin 4 g

propylene glycol 4 g

Polyoxyethylene hydrogenated castor oil 0.1 g

carboxy polymer 0.3 g

triethanolamine 0.3g

antioxidant appropriate amount

Spices appropriate amount

antiseptic appropriate amount

Purified water appropriate amount

According to a conventional gel preparation method, each component was added and dissolved in purified water, a fragrance was added, and then the above components were mixed to prepare a gel.

<Skin external preparation Preparation Example 3> Preparation of ointment

active substance 100 mg

cetostearyl alcohol 2 g

Self-emulsifying monostearic acid 2 g

stearic acid 1 g

beeswax 4 g

squalane 7 g

monostearin glycerin 3 g

Monostearate Sorbitan 1 g

Polysorbate 80 3 g

glycerin 5 g

propylene glycol 4 g

Spices appropriate amount

Vaseline remaining amount

According to a conventional method for preparing an ointment, each component was added to and dissolved in vaseline, a fragrance was added, and then the above components were mixed to prepare an ointment.

So far, with respect to the present invention, the preferred embodiments have been looked at. Those of ordinary skill in the art to which the present invention pertains will understand that the present invention may be implemented in a modified form without departing from the essential characteristics of the present invention. Therefore, the disclosed embodiments are to be considered in an illustrative rather than a restrictive sense. The scope of the present invention is indicated not in the foregoing description, but in particular in the claims, and all differences within the scope equivalent thereto should be construed as being included in the present invention.

Claims (6)

Black bean ( Glycine max seed) extract, green tangerine ( Citrus reticulata fruit) extract and turmeric ( Curcuma longa root) extract, comprising a, hair loss symptom prevention or improvement cosmetic composition.
The method of claim 1,
The black soybean extract is a cosmetic composition for preventing or improving hair loss symptoms, characterized in that the black bean extract is an extract of defatted Glycine max seed.
The method of claim 1,
The cosmetic is in the form of a lotion, emulsion, cream, skin, lotion, serum, essence, emulsion, powder, cosmetic ointment, spray, gel, pack, cleanser, soap, shampoo, conditioner, bathing agent, detergent or cone stick. A cosmetic composition for preventing or improving hair loss symptoms.
Black bean ( Glycine max seed ) extract, green tangerine ( Citrus reticulata fruit ) extract and turmeric ( Curcuma longa root ) extract, comprising a skin external composition for preventing or improving hair loss symptoms.
5. The method of claim 4,
The black soybean extract is a skin external composition for preventing or improving hair loss symptoms, characterized in that the black soybean oil meal (defatted Glycine max seed) extract.
5. The method of claim 4,
The external preparation for skin is an ointment, patch, gel, cream or spray, characterized in that the skin external application composition for preventing or improving hair loss symptoms.

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