KR20220118295A - 고 처리량 단일 세포 라이브러리, 및 이의 제조 방법 및 사용 방법 - Google Patents

고 처리량 단일 세포 라이브러리, 및 이의 제조 방법 및 사용 방법 Download PDF

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KR20220118295A
KR20220118295A KR1020217030969A KR20217030969A KR20220118295A KR 20220118295 A KR20220118295 A KR 20220118295A KR 1020217030969 A KR1020217030969 A KR 1020217030969A KR 20217030969 A KR20217030969 A KR 20217030969A KR 20220118295 A KR20220118295 A KR 20220118295A
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cells
nuclei
nucleic acid
cell
sequencing
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Korean (ko)
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제이 센듀어
다렌 쿠사노비치
리자 다자
프랑크 스티머스
앤드류 케네디
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일루미나, 인코포레이티드
유니버시티 오브 워싱톤
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q1/6813—Hybridisation assays
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation

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  • Zoology (AREA)
  • Wood Science & Technology (AREA)
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  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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KR1020217030969A 2019-12-19 2020-12-18 고 처리량 단일 세포 라이브러리, 및 이의 제조 방법 및 사용 방법 Pending KR20220118295A (ko)

Applications Claiming Priority (3)

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US201962950670P 2019-12-19 2019-12-19
US62/950,670 2019-12-19
PCT/US2020/066013 WO2021127436A2 (en) 2019-12-19 2020-12-18 High-throughput single-cell libraries and methods of making and of using

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KR20220118295A true KR20220118295A (ko) 2022-08-25

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US (1) US20220356461A1 (enExample)
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CN (1) CN114008199A (enExample)
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CA (1) CA3134746A1 (enExample)
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KR20230020977A (ko) 2020-06-09 2023-02-13 일루미나, 인코포레이티드 시퀀싱 라이브러리의 생성을 증가시키는 방법
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CN114121158A (zh) * 2021-12-01 2022-03-01 湖南大学 一种基于深度网络自适应的scRNA-seq细胞类型识别方法
WO2023137292A1 (en) * 2022-01-12 2023-07-20 Jumpcode Genomics, Inc. Methods and compositions for transcriptome analysis
CN115064220B (zh) * 2022-06-14 2025-05-16 浙江大学 一种单细胞的跨物种细胞类型鉴定方法
WO2024019988A1 (en) * 2022-07-18 2024-01-25 Cellsbin, Inc. Devices for biological analysis
CN118460688A (zh) * 2023-02-09 2024-08-09 中国人民解放军军事科学院军事医学研究院 多聚甲醛固定细胞的单细胞转录组及多重靶向蛋白质同时检测方法的建立
WO2024250155A1 (zh) * 2023-06-05 2024-12-12 清华大学 一种构建单细胞测序文库的方法
CN117802091B (zh) * 2023-12-29 2026-02-06 江苏农牧科技职业学院 一种地方鹅t2t基因组组装方法
CN120442620B (zh) * 2025-07-11 2025-10-31 良渚实验室 用于蛋白检测体系的寡核苷酸阻断剂及降低基于邻位反应的超灵敏蛋白检测体系背景的方法

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MX2021011847A (es) 2021-11-17
MX2025014294A (es) 2026-01-07
SG11202109486QA (en) 2021-09-29
BR112021019640A2 (pt) 2022-06-21
JP2023508792A (ja) 2023-03-06
JP7864297B6 (ja) 2026-07-10
EP3927824A2 (en) 2021-12-29
WO2021127436A2 (en) 2021-06-24
WO2021127436A3 (en) 2021-07-29
JP7864297B2 (ja) 2026-05-25
JP2025108660A (ja) 2025-07-23
AU2020407641A1 (en) 2021-09-23
IL286643A (en) 2021-12-01
CA3134746A1 (en) 2021-06-24
CN114008199A (zh) 2022-02-01
US20220356461A1 (en) 2022-11-10

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