JP7864297B6 - ハイスループット単一細胞ライブラリー、並びに製造方法及び使用方法 - Google Patents

ハイスループット単一細胞ライブラリー、並びに製造方法及び使用方法

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Publication number
JP7864297B6
JP7864297B6 JP2021557409A JP2021557409A JP7864297B6 JP 7864297 B6 JP7864297 B6 JP 7864297B6 JP 2021557409 A JP2021557409 A JP 2021557409A JP 2021557409 A JP2021557409 A JP 2021557409A JP 7864297 B6 JP7864297 B6 JP 7864297B6
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cells
cell
nucleic acid
index
nuclei
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JP2023508792A (ja
JP2023508792A5 (enExample
JP7864297B2 (ja
Inventor
ジェイ シェンドゥア，
ダレン クサノビッチ，
リザ ダザ，
フランク ジェイ． スティーマーズ，
アンドリュー ケネディー，
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WASHINGTON, University of
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WASHINGTON, University of
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2021557409A 2019-12-19 2020-12-18 ハイスループット単一細胞ライブラリー、並びに製造方法及び使用方法 Active JP7864297B6 (ja)

Priority Applications (1)

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JP2025069356A JP2025108660A (ja) 2019-12-19 2025-04-21 ハイスループット単一細胞ライブラリー、並びに製造方法及び使用方法

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US201962950670P 2019-12-19 2019-12-19
US62/950,670 2019-12-19
PCT/US2020/066013 WO2021127436A2 (en) 2019-12-19 2020-12-18 High-throughput single-cell libraries and methods of making and of using

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JP2025069356A Pending JP2025108660A (ja) 2019-12-19 2025-04-21 ハイスループット単一細胞ライブラリー、並びに製造方法及び使用方法

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CN (1) CN114008199A (enExample)
AU (1) AU2020407641A1 (enExample)
BR (1) BR112021019640A2 (enExample)
CA (1) CA3134746A1 (enExample)
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KR20230020977A (ko) 2020-06-09 2023-02-13 일루미나, 인코포레이티드 시퀀싱 라이브러리의 생성을 증가시키는 방법
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CN114121158A (zh) * 2021-12-01 2022-03-01 湖南大学 一种基于深度网络自适应的scRNA-seq细胞类型识别方法
WO2023137292A1 (en) * 2022-01-12 2023-07-20 Jumpcode Genomics, Inc. Methods and compositions for transcriptome analysis
CN115064220B (zh) * 2022-06-14 2025-05-16 浙江大学 一种单细胞的跨物种细胞类型鉴定方法
WO2024019988A1 (en) * 2022-07-18 2024-01-25 Cellsbin, Inc. Devices for biological analysis
CN118460688A (zh) * 2023-02-09 2024-08-09 中国人民解放军军事科学院军事医学研究院 多聚甲醛固定细胞的单细胞转录组及多重靶向蛋白质同时检测方法的建立
WO2024250155A1 (zh) * 2023-06-05 2024-12-12 清华大学 一种构建单细胞测序文库的方法
CN117802091B (zh) * 2023-12-29 2026-02-06 江苏农牧科技职业学院 一种地方鹅t2t基因组组装方法
CN120442620B (zh) * 2025-07-11 2025-10-31 良渚实验室 用于蛋白检测体系的寡核苷酸阻断剂及降低基于邻位反应的超灵敏蛋白检测体系背景的方法

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MX2025014294A (es) 2026-01-07
SG11202109486QA (en) 2021-09-29
BR112021019640A2 (pt) 2022-06-21
JP2023508792A (ja) 2023-03-06
KR20220118295A (ko) 2022-08-25
EP3927824A2 (en) 2021-12-29
WO2021127436A2 (en) 2021-06-24
WO2021127436A3 (en) 2021-07-29
JP7864297B2 (ja) 2026-05-25
JP2025108660A (ja) 2025-07-23
AU2020407641A1 (en) 2021-09-23
IL286643A (en) 2021-12-01
CA3134746A1 (en) 2021-06-24
CN114008199A (zh) 2022-02-01
US20220356461A1 (en) 2022-11-10

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