KR20220117559A - Cosmetic composition comprising Aloe fermented product - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising an aloe fermented product, and specifically, relates to a cosmetic composition comprising a product obtained by fermenting aloe with Lactobacillus plantarum. The cosmetic composition of the present invention utilizes aloe as a natural raw material, so that the cosmetic composition has almost no cytotoxicity to be used safely, and shows excellent antioxidant and anti-inflammatory activity to be helpful for alleviating skin aging or inflammatory skin diseases through skin aging prevention and skin inflammatory alleviation.

Description

Cosmetic composition comprising Aloe fermented product

The present invention relates to a cosmetic composition comprising fermented aloe, and more particularly, to a cosmetic composition comprising a fermented product of aloe with Lactobacillus plantarum.

The global cosmetic material market is expected to reach approximately $31.8 billion in 2023, and is reported to have a CAGR of 4.43% from 2017 to 2023. As the presence of Korean cosmetics in the global market has greatly increased, it is attracting attention as one of the world trends. Recently, the status of Korean cosmetics is expected to rise further as it expands its exports to China and ASEAN markets and advances into advanced markets such as the United States and Europe. It can be said that various technologies were implemented in the cosmetic industry due to the technological growth and development of the Korean cosmetic material industry, and the development of the cosmetic industry had a considerable influence on this.

The consumption craze of domestic natural cosmetics started to gradually increase along with the well-being craze following the US, Europe, and Japan, followed by the implementation of the cosmetic ingredient labeling system, and a study on the fact that chemical ingredients such as parabens, mineral oil, artificial colorants, and formaldehyde are harmful to the skin. As the results are announced, more and more customers are looking for products using clean and safe natural ingredients.

On the other hand, the occurrence of skin inflammatory diseases such as atopic dermatitis is increasing due to environmental pollution and increased use of chemicals due to industrialization. The demand is gradually increasing.

Accordingly, the present inventors tried to develop a cosmetic that can be used more safely by using natural raw materials, particularly aloe, and can effectively improve oxidation and inflammation of the skin.

Korean Patent Registration No. 10-2136090

Accordingly, the main object of the present invention is to provide a cosmetic composition that can be safely used using aloe, a natural raw material, and exhibit excellent functions such as antioxidant and anti-inflammatory.

According to one aspect of the present invention, there is provided a cosmetic composition comprising a fermented product of aloe fermented with Lactobacillus plantarum.

In the cosmetic composition of the present invention, the fermented product is preferably inoculated with the Lactobacillus plantarum in a medium containing aloe powder and fermented.

In the cosmetic composition of the present invention, the medium contains 0.1 to 1% (w/v) of aloe powder, 0.5 to 2% (w/v) of sucrose, and 0.05 to 0.5% (w/v) of yeast extract. ) is preferably an aqueous solution containing

In the cosmetic composition of the present invention, the fermentation is preferably made at 35 to 39 ℃ for 1 to 10 days.

In the cosmetic composition of the present invention, the cosmetic composition is preferably a cosmetic composition for preventing skin oxidation or improving skin inflammation.

The cosmetic composition of the present invention utilizes aloe, a natural raw material, and has almost no cytotoxicity, so it can be used safely. It can be of great help.

1 shows an aloe fermented product (lyophilized state) according to an embodiment of the present invention.
2 shows the results of the DPPH radical scavenging activity test for the fermented aloe of the present invention.
3 shows the results of a cytotoxicity test for the fermented aloe of the present invention.
4 shows the results of a test for inhibiting NO (nitric oxide) production of fermented aloe of the present invention. N: normal group, C: control group, ***p<0.001 vs N, ###p<0.001 vs C.

The cosmetic composition of the present invention is characterized in that it comprises a fermented product obtained by fermenting aloe with Lactobacillus plantarum .

Aloe is a perennial plant belonging to the genus Aloe of the Liliaceae botanically, and Aloe vera L. is preferably used in the present invention.

For fermentation in the present invention, a conventional fermentation method using microorganisms, particularly microorganisms of the genus Lactobacillus, in particular Lactobacillus plantarum, as fermentation microorganisms may be used. Preferably, a method for fermentation by inoculating a fermentation medium containing aloe with a fermentation microorganism is used.

In the present invention, aloe is preferably powdered and used for fermentation. At this time, a conventional method of pulverizing plant materials for pulverization, for example, a method of pulverizing by drying and pulverizing may be used. In this case, as a drying method, methods such as natural drying, hot air drying, and freeze drying may be used.

Lactobacillus plantarum used as a fermentation microorganism in the present invention can be cultured by a general method of culturing a microorganism of the genus Lactobacillus. For example, it can be cultured using MRS medium, preferably cultured at 30 to 40 ℃. In the present invention, preferably, the Lactobacillus plantarum KCTC 3104 strain is used.

In the present invention, for inoculation, for example, a method of adding a culture cell, a solid culture, or a culture solution of the fermenting microorganism to a fermentation medium containing aloe may be used. In this case, the fermenting microorganism may be added to be, for example, 10 to 10 ¹⁰ cell/ml, 10 ² to 10 ⁹ cell/ml, 10 ³ to 10 ⁸ cell/ml, or 10 ⁴ to 10 ⁷ cell/ml.

In the present invention, for efficient fermentation and excellent functionality of aloe, a fermentation medium containing 0.1 to 1% (w/v) of aloe powder is preferably used. More preferably a fermentation medium containing 0.2 to 0.8% (w/v) of aloe powder, more preferably a fermentation medium containing 0.3 to 0.7% (w/v) of aloe powder, more preferably aloe powder A fermentation medium containing this 0.4 to 0.6% (w/v) is used. Preferably, a fermentation medium containing sucrose and yeast extract along with aloe powder is used. At this time, the sucrose content in the fermentation medium is preferably 0.5 to 2% (w/v), more preferably 0.6 to 1.5% (w/v), more preferably 0.7 to 1.3% (w/v), more preferably Preferably 0.8 to 1.2% (w / v), the content of the yeast extract is preferably 0.05 to 0.5% (w / v), more preferably 0.07 to 0.4% (w / v), more preferably 0.1 to 0.3% (w/v).

In the present invention, for fermentation, a method of inoculating Lactobacillus plantarum into an aloe-containing fermentation medium and maintaining it at a constant temperature for a predetermined period may be used. Preferably at 35 to 39 ° C, more preferably at 36 to 38 ° C after inoculation, preferably for 1 to 10 days, more preferably for 3 to 9 days, more preferably for 6 to 8 days Fermented in a way

The cosmetic composition of the present invention can exhibit excellent antioxidant and anti-inflammatory effects by including the fermented aloe as described above. Accordingly, the cosmetic composition of the present invention may be a cosmetic composition for preventing skin oxidation or improving skin inflammation.

In the present invention, the fermented aloe is an active ingredient for functionality, that is, an antioxidant or anti-inflammatory effect. The cosmetic composition of the present invention may be the fermented aloe itself as described above, and other cosmetically acceptable carriers or additives. may include more. In this case, the carrier also includes a pharmaceutically acceptable carrier. In addition, the cosmetic composition of the present invention can be formulated in various forms, for example, moisture cream, lotion, nutrient lotion, nutrient cream, massage cream, pack and nutrient essence.

The cosmetic composition of the present invention may further include other functional substances in addition to the fermented aloe as described above. In this case, the functional material may be, for example, a material processed by other methods of aloe or an extract or fermented product of a raw material other than aloe. In addition, the cosmetic composition of the present invention may include only fermented aloe as an active ingredient, and may not include other functional substances.

The cosmetic composition of the present invention may include, for example, 0.1 to 99% by weight of the fermented aloe, but is not limited thereto.

Hereinafter, the present invention will be described in more detail through examples. These examples are only for illustrating the present invention and should not be construed as limiting the scope of the present invention by these examples.

[Example]

Example 1. Preparation of aloe fermented product

Fermentation medium containing 0.5% (w/v) of Aloe vera L. powder (dry powder), 1.0% (w/v) of sucrose, and 0.2% (w/v) of yeast extract in water prepared, and then inoculated with Lactobacillus plantarum (KCTC 3104) and then fermented at 37° C. for a week to obtain a fermented product.

At this time, Lactobacillus plantarum used as a fermentation strain was cultured in MRS medium and used for inoculation.

Formulation Example 1. Cream preparation

Aloe fermented product prepared in Example 1, drumstick leaf (moringa leaf) extract, glycerin, centella asiatica leaf water, olive oil, cetearyl alcohol, purslane extract, sunflower seed oil, purified water, avocado oil, caprylic /Capric triglyceride, Matricaria flower water, isoamyllaurate, candelilla wax, propanediol, C12-20 alkyl glucoside, C14-22 alcohol, glyceryl stearate, tocopherol, jojoba seed oil, shea butter, Sunflower Seed Oil, Sweet Almond Oil, Scented Geranium Oil, Mandarin Peel Oil, Grapefruit Peel Oil, Lavender Oil, West Australian Sandalwood Wood Oil, Ylang Ylang Flower Oil, Bitter Orange Leaf, Twig Oil, Chamomile Flower Oil, Sliced Extract, Hydrogenated Lecithin, Golden Extract, Xanthan Gum, Peony Root Extract, Usnia Extract, Chopi Tree Fruit Extract, Walnut Flower Extract, Dipotassium Glycyrrhizate, Betaine, Oil-soluble Licorice Extract, Adenosine, Sodium Hyaluronate, Limonene , linalool, citronellol, geraniol, and farnesol are mixed to prepare a cream formulation.

Experimental Example 1. Functional evaluation

The fermented aloe prepared in Example 1 was freeze-dried and then stored frozen at -20°C and used in the experiment. The sample to be used in the experiment was used after dissolving in distilled water at a concentration of 100 mg/ml.

1-1. DPPH radical scavenging activity

20 μl of the sample solution and 80 μl of DPPH (2,2-Diphenyl-1-picrylhydrazyl, Sigma-Aldrich, St Louis, USA) solution were mixed in a 96-well plate, left at room temperature for 30 minutes, and spectrophotometric ELISA at 517 nm , and ascorbic acid (Sigma-Aldrich), one of the water-soluble vitamins, was used as a positive control for activity comparison.

As a result, as shown in FIG. 2 , the fermented aloe exhibited DPPH radical scavenging activity in a concentration-dependent manner.

1-2. Cell culture used in the experiment

RAW 264.7 (TIB-71) macrophages used in the experiment were purchased from ATCC (American type cell collection, USA), and 10% FBS (Fetal Bovine Serum; Gibco, USA) in DMEM (Dulbecco's Modified Eagle's Medium; Gibco, USA) medium. ) and 1% penicillin (100U/ml)/streptocycin (100㎍/ml) were added, and then cultured at 37°C and 5% CO ₂ conditions.

1-3. cytotoxicity

The survival rate of the sample was tested by MTT ([3-(4,5-methylthiazol-2-yl)-2,5-diphenyltetrazolium bromide], Sigma Chemical Co., St. Louis MO, USA) method. Cells were aliquoted in a 96-well plate at a concentration of 1 × 10 ⁴ cell/well, and the medium was removed after culturing for 24 hours. Samples diluted for each concentration were added and incubated for 24 hours, then 10 μl of MTT solution (5 mg/ml) was added to each well and further cultured for 4 hours. After removing the medium and adding 100 μl/well of DMSO (dimethylsulfoxide; Sigma Chemical Co., St. Louis MO, USA), the formazan generated in the cells was stirred for 15 minutes to elute, and then using an ELISA reader Absorbance was measured at 590 nm.

To check the presence or absence of cytotoxicity of the aloe fermented product, RAW 264.7 cells were treated with each sample at a concentration of 1, 10, 50, 100, 150, 200, 500, and 1000 μg/ml, and then the cell viability rate was measured. As a result, as shown in FIG. 3, even up to 1000 μg/ml showed a cell viability of 80% or more.

1-4. Inhibiting NO (nitric oxide) production

RAW 264.7 cells were aliquoted in a 6-well plate at a concentration of 5 × 10 ⁵ cells/well, and the medium was removed after culturing for 24 hours. Samples diluted by concentration were pre-treated for 1 hour, treated with LPS (lipopolysaccharide, 1 μg/ml), and cultured in an incubator at 37° C., 5% CO ₂ for 18 hours. After 18 hours of sample treatment, 50 μl of the cell culture supernatant and 50 μl of Griess reagent (1% sulfanilamide + 0.1% α-naphthylamide in 2.5% phosphoric acid) were mixed and reacted in a 96-well plate for 10 minutes, followed by an ELISA reader. Absorbance was measured at 540 nm. NaNO ₂ was quantified using a standard solution.

In order to measure the ability of the aloe fermented product to inhibit LPS-induced NO production in RAW 264.7 cells, RAW 264.7 cells were treated with aloe fermented at concentrations of 125, 250, and 500 μg/ml, respectively. As a result, as shown in FIG. 4 . A concentration-dependent inhibitory efficacy of the aloe ferment was observed.

Claims (5)

A cosmetic composition comprising a fermented product obtained by fermenting aloe with Lactobacillus plantarum. According to claim 1,
The composition of claim 1, wherein the fermented product is inoculated with the Lactobacillus plantarum in a medium containing aloe powder and fermented. 3. The method of claim 2,
The medium is an aqueous solution containing 0.1 to 1% (w/v) of aloe powder, 0.5 to 2% (w/v) of sucrose, and 0.05 to 0.5% (w/v) of yeast extract. . 3. The method of claim 2,
The fermentation is made for 1 to 10 days at 35 to 39 ℃, the composition. The method of claim 1,
The cosmetic composition is a cosmetic composition for preventing skin oxidation or improving skin inflammation, the composition.

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