KR20220078267A - Animal feed additives comprising daphne genkwa callus or sprout - Google Patents

Abstract

The present invention provides an additive for animal feed, characterized in that it contains a powder or an extract thereof from Azalea callus or seedlings as an active ingredient.

Description

Additive for animal feed containing red bean callus or seedlings as an active ingredient {ANIMAL FEED ADDITIVES COMPRISING DAPHNE GENKWA CALLUS OR SPROUT}

The present invention relates to an additive for animal feed, and more particularly, it is an active ingredient of Azalea callus or seedlings capable of providing high-quality meat while economical breeding through the action of improving meat quality, improving feed efficiency, and improving immune activity. It relates to an additive for animal feed containing

In general, the breeding of livestock is a trend that has been commercialized and enlarged in recent years, and livestock raised according to this trend is also densely reared in a limited space. And, the feed of livestock densely reared in the limited space as described above uses a lot of artificial feed, and the artificial feed is easily purchased and the demand for it is greatly increased due to the advantages of convenient use.

Most of the artificially formulated feed contains nutrients such as crude protein, crude fat, crude fiber, crude flour, calcium and phosphorus, and it is a drug that affects the metabolism of livestock. Agents, inorganic and organic acids are separately added and used. On the other hand, separate antibacterial materials, antibiotics, and preservatives are added to artificially formulated feeds to prevent diseases and improve feed efficiency, and recently, lactic acid bacteria are also added for the same purpose. Injections may also be administered directly.

In addition, a TMR (Total mixed ration) feeding method to increase the digestion efficiency of livestock and a TMF (Total mixed fermented ration) feeding method through a pre-fermentation process in order to maximize the digestion efficiency of livestock before feeding have been implemented. However, in the case of the compounded feed or the compounded fermented feed that has been conventionally used in these specification methods, the digestion efficiency is not as great as expected, and there has been a problem in that the quality grade decreases due to excessive input of antibacterial agents, antibiotics, and preservatives.

Accordingly, there is a need to develop an additive for animal feed that can improve meat quality, improve feed efficiency, and improve immune activity at the same time.

The present invention has been devised to solve the problems of the prior art as described above, and its purpose is to provide high-quality meat while economical breeding is possible through the action of improving meat quality, improving feed efficiency, and improving immune activity. It is to provide an additive for possible animal feed.

The technical problem of the present invention as described above is achieved by the following means.

(1) An additive for animal feed, characterized in that it contains a powder or extract of Azalea callus or seedlings as an active ingredient.

(2) in (1) above,

An additive for animal feed, characterized in that the animal is pig, cattle or poultry.

(3) in (1) above,

Additive for animal feed, characterized in that it further comprises a powder or extract of watermelon callus or seedlings.

(4) in (1) above,

The callus is characterized in that each section of the leaf, stem, and root of the azalea was induced in a dark culture room by basal MS medium supplemented with BA 1.0-3.0 mg/L and NAA 0.1-0.3 mg/L. additives for animal feed.

(5) in (1) above,

The seedling is an additive for animal feed, characterized in that it is within 2 months after germination.

(6) An animal breeding method, characterized in that the animal feed additive according to one of the above (1) to (5) is fed to the animal and used.

According to the present invention as described above, economical breeding is possible through the action of improving meat quality, improving feed efficiency, and improving immune activity, and it provides the effect of providing high-quality meat.

The additive for animal feed according to the present invention contains Azalea callus or seedlings, powder or extract thereof as an active ingredient.

The additive for animal feed according to the present invention enables economical breeding and the provision of high-quality meat through the action of improving meat quality, improving feed efficiency, and improving immune activity.

Hereinafter, the content of the present invention will be described in more detail with reference to Examples.

Animals in the present invention include poultry such as pigs, cattle, chickens, etc., preferably pigs, cattle, chickens, but is not necessarily limited thereto.

In the present invention, the Azalea callus is derived from the tissues of leaves, stems, and roots, and is not limited to a specific site. Preferably, each section of the leaves, stems, and roots of the Azalea is added with BA 1.0 to 3.0 mg/L, preferably 2.0 mg/L and NAA 0.1 to 0.3 mg/L, preferably 0.2 mg/L. Use the induced callus in a culture room in a dark state at 25°C by basal MS medium.

In the present invention, the callus or seedlings (preferably 2 months or less after germination), the powder thereof, of the Azalea is preferably used in a dried state. The dried state includes dried products of callus or seedlings obtained by conventional methods such as hot air drying, natural drying, and freeze drying, or powders thereof. Preferably, it is good to use a freeze-dried state. Hot air drying is good if the callus is dried in the range of 40 ~ 65 ℃, freeze-drying is preferably performed at -40 ℃ ~ -70 ℃ of the callus.

The Azalea callus extract according to the present invention includes a hot water extract, an ethanol extract, an extract using a mixture of water and ethanol, or a supercritical extract.

In the case of hot water extract, it is preferable to add 1 to 10 times water and extract for 1 to 12 hours at 1 to 2 atmospheres and 80 to 120°C. In the case of the ethanol extract, an extraction solvent consisting of 50 to 100% ethanol may be used, and the supercritical extraction may be performed under a pressure of 250 to 350 bar using ethanol as an extraction solvent.

The amount of the additive for animal feed according to the present invention is not particularly limited, and it is preferably added in an amount of 0.1 to 10% by weight, preferably 1.0 to 5.0% by weight, based on the total weight of the feed composition. When it is added within the above range, it is most effective especially in terms of general components of meat, carcass performance, breeding performance, and fatty acid performance.

The additive for animal feed according to the present invention may further include an extract of the leaves, stems or roots of Azalea and/or watermelon.

Hereinafter, the contents of the present invention will be described in more detail with reference to Examples and Experimental Examples. However, these Examples and Experimental Examples are only exemplified to help the understanding of the present invention, and the scope of the present invention is not limited thereto.

[Example] Induction of callus

Callus was induced in a dark culture room at 25° C. by placing 30 slices of each leaf, stem, and root of the azalea on MS basal medium supplemented with BA 2.0 mg/L and NAA 0.2 mg/L. About 6-7 weeks after tissue dentition, callus began to form, and as this callus turned brown, a new callus was formed on some surfaces. The callus was continuously induced and proliferated by subculture with the same medium at intervals of 4 weeks.

[Example 1] Preparation of powder

Each section of the petals (Example 1-1), leaves (Example 1-2), stems (Examples 1-3), and roots (Examples 1-4) of the Azalea and watermelon obtained in Example 1 The callus derived from was lyophilized and powdered. Also, separately from the callus, the seedlings (10 days after germination) of Azalea were freeze-dried and powdered (Example 1-5).

[Example 2] Preparation of callus extract

Callus derived from each section of the petal (Example 2-1), leaf (Example 2-2), stem (Example 2-3), and root (Example 2-4) obtained in Example 1 Extraction was performed with 70% ethanol to obtain a callus extract. Also, separately from the callus, the seedlings of the azalea (10 days after germination) were powdered and extracted with 70% ethanol to obtain a callus extract (Example 2-5).

[Example 3] Preparation of water extract

Callus derived from each section of the petal (Example 3-1), leaf (Example 3-2), stem (Example 3-3), and root (Example 3-4) obtained in Example 1 above Extraction was performed with 70% ethanol to obtain a callus extract. Separately from the callus, seedlings of water subsistence (10 days after germination) were powdered and extracted with 70% ethanol to obtain a callus extract (Example 3-5).

[Experimental Example 1]

As a basic feed, a feed containing 18.0% of crude protein and 0.9% of lysine was used as shown in Table 2 below, and samples prepared according to Examples 1, 2, and 3 were prepared for each experimental group (of Experimental Group 3). In case, 10% by weight was added to the basic feed of each 5% by weight of the result prepared in Examples 1 and 3), and the effect after feeding was investigated. In each Example, 10 young pigs weighing 28.1 ㅁ0.1 kg were used as an experimental group, and 10 young pigs without the addition of powder or extract of callus or seedlings of Azalea as control group 1 were used as control group 1, and mature plant red beans were used as test pigs. 10 young pigs bred by adding the same amount of ethanol extract of flower petals were used as Control 2. Table 1 shows the results of measuring the breeding period, feed intake, and daily average weight during breeding until the average weight reached 100 to 115 kg. The composition of the feed fed during the breeding period is shown in Table 2.

breeding period Feed intake average weight 1 day weight gain Feed intake/ body weight kg experimental group Experimental group 1 Example 1-1 98 days 248 kg 113.8kg 874g 2.18 Example 1-2 99 days 243 kg 113.5kg 862g 2.18 Examples 1-3 100 days 242 kg 114.1kg 860g 2.14 Examples 1-4 98 days 245 kg 113.9kg 875g 2.15 Examples 1-5 97 days 240 kg 114.2kg 887g 2.10 Experimental group 2 Example 2-1 100 days 251 kg 114.6kg 865g 2.19 Example 2-2 101 days 250 kg 114.5kg 855g 2.18 Example 2-3 101 days 248 kg 114.3kg 853g 2.18 Example 2-4 102 days 249 kg 114.0kg 842g 2.18 Example 2-5 98 days 246 kg 114.9kg 885g 2.14 Experimental group 3 Example 1-1/3-1 96 days 250 kg 114.8kg 903g 2.18 Example 1-2/3-2 97 days 251 kg 115.0kg 895g 2.18 Examples 1-3/3-3 96 days 252 kg 115.0kg 905g 2.19 Examples 1-4/3-4 98 days 252 kg 114.9kg 885g 2.19 Examples 1-5/3-5 94 days 253 kg 115.1kg 925g 2.20 control Control 1 109 days 269 kg 111.3kg 763g 2.42 Control 2 111 days 281 kg 111.0kg 746g 2.53

Pig feed ingredients crude protein 18% george 5% crude fiber 10% views 10% calcium 0.55% sign One% lysine 0.9%

As can be seen from Table 1 above, as a result of feeding the breeding pigs by adding Azulberry callus to the feed, in the case of the experimental group, the breeding period was shortened by about 10 days or more until the average weight reached 114 kg, and the feed compared to the weight gain Intake was reduced by 12%. In the case of Control 2, it can be seen that the effect is lower than that of Control 1 in terms of feed efficiency or weight gain, contrary to expectations. In the case of the example of the present invention, it was confirmed that the daily weight gain was higher than that of the control group, thereby confirming that the feed absorption efficiency was high.

[Experimental Example 2] Meat quality improvement

In Experimental Example 1, pork belly and neck meat were separated from test pigs fed feed for a total of 6 months, and the results of measuring pH, water holding capacity, and heating loss, which are factors that generally affect pork quality, are shown in Table 3 below. same.

pH water holding capacity Heat loss (%) pork belly Control 1 5.62 58.2 25.3 Control 2 5.63 57.8 26.2 Example 1-1 5.42 67.3 20.2 Examples 1-5 5.36 71.5 18.2 Example 2-1 5.43 69.8 19.9 Example 1-1/3-1 5.44 70.1 19.1 throat Control 1 5.71 57.6 24.8 Control 2 5.67 57.3 25.5 Example 1-1 5.39 66.8 20.3 Examples 1-5 5.36 68.7 19.0 Example 2-1 5.38 67.9 20.2 Example 1-1/3-1 5.40 69.2 19.7

pH affects meat color, water retention, protein solubility, and microbial decomposition rate. As a result of the experiment of the present invention, pH was in the range of 5.36 to 5.44, similar to general values, and water retention was higher in both pork belly and neck meat than in the control group. , the heating loss was lower than that of the control in both the pork belly and the neck, and in particular, Examples 1-5 using seedlings showed the best results. In the case of Control 2, it can be seen that the quality is somewhat lower than that of Control 1. As described above, it can be seen that the embodiment according to the present invention has a high water holding capacity and a low heating loss, so that it can be evaluated as pork having excellent quality due to a high grade of meat quality.

[Experimental Example 3] Sensory test

A sensory test was performed using the sirloin of the pig obtained in Experimental Example 2. According to the NPPC standard, the sensory test for meat quality is performed by 9 sensory testers according to the NPPC standard, 1 to 5 (the bigger the number, the clearer), the intramuscular fat: 1 to 5 (the larger the number, the more uniform the marbling distribution), and the hardness: 1 to 5 (the number is the number) The larger the value, the better the elasticity of the meat). The results are shown in Table 4 below, and the average value was used 5 times.

six colors intramuscular fat elasticity Control 1 2.3 1.3 1.4 Control 2 2.4 1.2 1.2 Example 1-1 2.5 2.0 2.2 Examples 1-5 2.6 2.4 2.6 Example 2-1 2.5 2.2 2.3 Example 1-1/3-1 2.5 2.4 2.4

According to the experimental results, all of the Examples showed superior meat color, intramuscular fatness and elasticity compared to the control, and in particular, the experiments using the seedlings of Examples 1-5 showed superior both intramuscular fatness and elasticity.

[Experimental Example 4] Immune activity

cell culture

The IL-2 dependent NK cell line NK92 (human NK lymphoma) was purchased from the American Type Culture Collection (ATCC). NK92 cells contained 20% FCS (HyClone, Logan, UT), 2 mM L-glutamate, 100 ug/ml penicillin, 100 ug/ml streptomycin (Life Technologies) and 100 U/ml IL-2 (Chiron, Emeryville, CA) supplemented with α-MEM (Life Technologies, Karlsruhe, Germany) medium was maintained.

ELISA assay for IFN-γ production

Quantification of human IFN-γ was performed using a commercially available mAb (Endogen) according to the manufacturer's instructions. After incubation at 37° C. for 18 hours, the cells were removed and the supernatant was collected. The results are shown in Table 5 below, expressed as the mean ± SEM of the values performed three times.

Sample Average Standard Deviation Example 1
(Treatment concentration: 50 μg/ml) Example 1-1 0.912 0.064 Example 1-2 0.909 0.048 Examples 1-3 0.911 0.053 Examples 1-4 0.907 0.037 Examples 1-5 0.921 0.053 Example 2
(Treatment concentration: 50 μg/ml) Example 2-1 0.901 0.044 Example 2-2 0.903 0.031 Example 2-3 0.900 0.028 Example 2-4 0.898 0.050 Example 2-5 0.915 0.055 Example 1+Example 3
(Treatment concentration: 25 μg/ml each) Example 1-1/3-1 0.952 0.015 Example 1-2/3-2 0.953 0.011 Example 1-3/3-3 0.948 0.020 Example 1-4/3-4 0.936 0.022 Example 1-5/3-5 0.963 0.021 Control 3 (DMSO-treated) ^* 0.712 0.045 Control 4 (treatment concentration: 50 μg/ml) ^** 0.799 0.068

^{*, **} Control 3: No treatment (DMSO only), Control 4: 70% ethanol extract treatment of the petals of a mature plant, Azulaceae.

[Experimental Example 5] Influenza virus infection inhibition experiment in mice

(1) Experimental method

1) 4-5 week old female Balb/c mice were divided into control group (5 mice) and Example 1, Example 2, Example 1+Example 3 administration group, that is, an experimental group (5 mice each).

2) In the case of the experimental group, about 100 μg/ml was administered orally every day for 5 days, and a lethal dose of PR8 virus (10LD50) was administered intranasally to the control group and the administration group to give an attack inoculation. ㎍ / ㎖ was administered orally every day for 5 days, and the mouse survival experiment was performed until the mice were killed.

(2) Experiment result

As shown in Table 2 below, while the control mice were all killed within 7 days after the virus challenge, no deaths were found in the mice of the experimental group. These results are considered that the experimental group suppressed the infection and proliferation of influenza virus by increasing the mouse's innate immunity.

Sample lethal head Example 1
(Treatment concentration: 100 μg/ml) Example 1-1 0 Example 1-2 0 Examples 1-3 0 Examples 1-4 0 Examples 1-5 0 Example 2
(Treatment concentration: 100 μg/ml) Example 2-1 0 Example 2-2 0 Example 2-3 0 Example 2-4 0 Example 2-5 0 Example 1+Example 3
(Treatment concentration: 50 μg/ml each) Example 1-1/3-1 0 Example 1-2/3-2 0 Example 1-3/3-3 0 Example 1-4/3-4 0 Example 1-5/3-5 0 Control 3 (DMSO) 5 Control 4 (treatment concentration: 100 μg/ml) 4

As described above, although described with reference to preferred embodiments of the present invention, those skilled in the art can variously modify and change the present invention within the scope without departing from the spirit and scope of the present invention described in the claims below. You will understand that it can be done.

Claims (6)

An additive for animal feed, characterized in that it contains powder or extracts thereof as an active ingredient of Azalea callus or seedlings. The method of claim 1,
An additive for animal feed, characterized in that the animal is pig, cow, or chicken. The method of claim 1,
An additive for animal feed, characterized in that it further comprises a powder or extracts thereof of watermelon callus or seedlings. The method of claim 1,
The callus is characterized in that each section of the leaf, stem, and root of the azalea was induced in a dark culture room by basal MS medium supplemented with BA 1.0-3.0 mg/L and NAA 0.1-0.3 mg/L. additive for animal feed. The method of claim 1,
The seedling is an additive for animal feed, characterized in that it is within 2 months after germination. An animal breeding method, characterized in that the animal feed additive of any one of claims 1 to 5 is fed to the animal and used.