CN110786424A - Application of magnolol and/or honokiol in poultry breeding - Google Patents
Application of magnolol and/or honokiol in poultry breeding Download PDFInfo
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- CN110786424A CN110786424A CN201910948167.3A CN201910948167A CN110786424A CN 110786424 A CN110786424 A CN 110786424A CN 201910948167 A CN201910948167 A CN 201910948167A CN 110786424 A CN110786424 A CN 110786424A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/111—Aromatic compounds
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The invention discloses application of magnolol and/or honokiol in poultry breeding, and belongs to the technical field of livestock breeding. The magnolol and/or honokiol is applied in daily ration of domestic fowl, and can promote intestinal villus development of fowl, and improve intestinal villus height and ratio of villus/crypt; and can obviously improve the adverse effects of common intestinal pathogenic bacteria infection, stress and the like of the poultry on the intestinal tract and the production performance of the chicken; the invention also discloses a feed additive which takes magnolol and/or honokiol as main components and replaces antibiotics.
Description
Technical Field
The invention belongs to the technical field of livestock and poultry breeding, and particularly relates to application of magnolol and/or honokiol in a feed additive in poultry breeding.
Background
Since the 40 th of the 20 th century, antibiotics are widely applied to livestock and poultry breeding as feed additives, can reduce the diarrhea rate of animals under certain conditions, regulate intestinal microbial flora, inhibit pathogenic bacteria and host competitive nutrient substances, and accordingly improve the growth performance of the animals and the utilization rate of the feed.
Statistically, among the currently used feed antibiotics in the world, 42% of the growth-promoting antibiotics account for the growth, and only 18% of the antibiotics account for the treatment. The phenomenon of antibiotic abuse is widespread. The long-term use of antibiotics can cause the drug resistance problem of pathogenic bacteria, also can destroy the micro-ecological balance in the intestinal tracts of livestock and poultry, and cause endogenous infection or double infection, so that the cellular immunity and the humoral immunity of the livestock and poultry are reduced, and the production performance is influenced; antibiotics generate residues in livestock and poultry products such as meat, eggs and milk, directly threaten the health and safety of human bodies, and have adverse effects on public health.
With the increasing concern of human beings on food safety, safety problems such as antibiotic and chemical drug residues become more and more prominent, and the development of the feed industry and the breeding industry is seriously hindered.
The intestinal health is the most important factor for the healthy growth of livestock and poultry. Therefore, the nutrition regulation and control means can promote the intestinal health degree, the immunologic function and the nutrition absorption function, improve the intestinal microecology, and is an important means for ensuring the organism health of livestock and poultry and improving the production performance of livestock and poultry.
Magnolol and honokiol are main active ingredients of cortex magnoliae officinalis, and have multiple physiological functions of resisting oxidation, inflammation and tumor. Previous research results prove that magnolol and honokiol have the following effects on common intestinal pathogens of chickens in vitro: the escherichia coli, the salmonella pullorum and the clostridium perfringens have obvious inhibiting effect; magnolol and honokiol can also be used for improving livestock and poultry intestinal immunity, promoting intestinal mucosa development, maintaining digestive tract microecological balance, and improving tissue antioxidant ability to ensure livestock and poultry intestinal health. Therefore, the development of the feed additive which takes magnolol and honokiol as main active ingredients and can replace antibiotics is of great significance for promoting the healthy development of the livestock industry.
Disclosure of Invention
The invention aims to provide a method for effectively promoting the development of intestinal mucosa, improving the oxidation resistance of the intestinal tract and effectively relieving the intestinal injury caused by pathogenic bacteria in the intestinal tract of poultry aiming at the health of the intestinal tract of the poultry. The invention relates to an application of magnolol and/or honokiol in preparing a feed additive for improving the intestinal health of poultry.
In order to achieve the purpose, the invention provides the following technical scheme: application of magnolol and/or honokiol in poultry breeding, and application of magnolol and/or honokiol in feed additives for promoting intestinal villus development and improving intestinal mucosa oxidation resistance.
Preferably, the magnolol and/or honokiol is applied to poultry breeding, and the magnolol and/or honokiol is applied to feed additives for relieving poultry intestinal pathogenic bacteria infection, stress-caused intestinal injury and production performance reduction.
Preferably, the magnolol and/or honokiol is applied to poultry breeding, and the magnolol and/or honokiol is applied to feed additives for reducing feed conversion ratio.
Preferably, the magnolol and/or honokiol are/is applied to poultry breeding, and the feed additive containing magnolol and honokiol is used for daily ration of broiler chickens, wherein the weight ratio of magnolol to honokiol is 1: 2-3.
Preferably, the magnolol and/or honokiol are/is applied to poultry breeding, the feed additive containing magnolol and honokiol is used for daily ration of laying hens, and the weight ratio of magnolol to honokiol is 1: 0.5-1.
Preferably, the feed additive containing magnolol and/or honokiol comprises, by weight, 0-30 parts of magnolol, 0-30 parts of honokiol, 5-10 parts of organic acid, 5-10 parts of functional oligosaccharide, 5-10 parts of probiotics, 1-5 parts of vitamin E and 40-75 parts of a carrier.
Preferably, the feed additive containing magnolol and/or honokiol as described above has a content of magnolol and/or honokiol of 20-30%.
Preferably, the feed additive containing magnolol and/or honokiol as described above has a mass concentration of 0.5-1kg/t in the feed.
Preferably, the feed additive containing magnolol and/or honokiol as described above, wherein the organic acid is any one or more of lactic acid, formic acid, propionic acid, butyric acid, citric acid, fumaric acid and salts thereof;
the functional oligosaccharide is any one or more of mannan oligosaccharide, chitosan oligosaccharide and fructooligosaccharide;
the probiotics are any one or more of bacillus coagulans, bacillus subtilis, clostridium butyricum and lactobacillus;
the carrier is any one of wheat bran, defatted rice bran and rice hull powder.
Depending on the purpose of use, magnolol and/or honokiol, when applied to a pharmaceutical or feed additive for improving the intestinal health of poultry, may contain, in addition to the compositions of the preferred embodiments listed above, other natural or synthetic substances having a synergistic effect on their basic active ingredients.
Compared with the prior art, the invention has the following advantages:
(1) the invention discovers new application of known compounds magnolol and honokiol in promoting intestinal development and ensuring intestinal health, and develops new application fields.
(2) The invention is a safe and efficient feed additive, improves the production performance of livestock and poultry by regulating and controlling the intestinal health, has no side effect on the livestock and poultry, and is not easy to generate drug resistance.
(3) According to the invention, appropriate amounts of organic acid, functional oligosaccharide, vitamin E and probiotics cooperate with magnolol and honokiol to exert biological activity, so that intestinal villus development is promoted, and poultry production performance is improved.
Detailed Description
In order to better understand the essence of the present invention, the following examples are given in more detail, but the following examples are only illustrative, and the present invention is not limited by these examples. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.
Example 1
320 healthy 1-day-old Aijia (AA) broilers with an average weight of 46 +/-2.5 g were selected and randomly divided into 4 treatments, each of which was repeated 8 times, namely a control group, a test group 1, a test group 2 and a test group 3. The breeding mode is cage culture. Control group and test group 1 were fed basal diet (see table 1 for nutritional needs of american NRC (2012) broiler chickens, and the composition of the diet), test group 2 was fed basal diet +300mg/kg honokiol, and test group 3 was fed basal diet +300mg/kg magnolol. Test groups 1, 2 and 3 carried out Salmonella pullorum challenge treatment on broilers at 7 days old (feeding with 10 days old)8CFU/ml 1ml of Salmonella pullorum culture solution), and the test period is 21 days, and the material consumption in the test period is recorded and weighed. And after the test is finished, measuring the broiler chicken production performance index. On the 14 th day and the 21 st day of the experimental period, 1 broiler chicken is randomly selected for slaughtering each time, and 1 cm of empty field and ileum anterior segment are collectedAnd (3) putting the rice tissues into the tissue fixing solution for 24 hours, then making tissue sections and HE dyeing, observing the intestinal villus form under a microscope, and measuring the villus length, the crypt depth and the intestinal wall thickness. The empty field and the ileum mucosa were scraped off, and the antioxidant capacity of the intestinal mucosa was measured, and the results are shown in tables 2 to 4.
TABLE 1 basic ration composition for different stages of broiler chickens
TABLE 2 Salmonella pullorum challenge test Productivity of broiler chickens
TABLE 3 intestinal mucosa structure of broiler (14 days old)
Table 4 intestinal mucosa structure of broiler (21 days old)
The result shows that the salmonella attacking can obviously reduce the growth performance of young broilers and the length of villus, but the application of the invention can obviously protect the intestinal villus damage of the broilers, and the height of the villus is superior to that of an unabridged control group.
Example 2
400 healthy 1-day-old Aijia (AA) broilers with the average weight of 46 +/-2.5 g are selected and randomly divided into 5 treatments, wherein each treatment is 8 times of repetition, and the treatments are respectively a control group and a test group 1, 2, 3 and 4. The breeding mode is ground flat breeding. Control group was fed basal diet (see us NRC (2012) broiler nutritional requirements, composition of diet see table 1), testGroup 1 was fed basal diet +200mg/kg magnolol; test group 2 was fed basal diet +200mg/kg honokiol; test group 3 fed basic ration and 0.1% feed additive (comprising 10 parts of magnolol, 10 parts of honokiol, 5 parts of sodium butyrate and 5 parts of mannan oligosaccharide, 10 parts of honokiol, and the like105 portions of CFU/g bacillus coagulans, 1 portions of vitamin E and 64 portions of rice hull powder), and a test group 4 feeds basic ration and 0.1% feed additive (the components and the parts by weight are 10 portions of magnolol, 10 portions of honokiol and 80 portions of rice hull powder). The test period is 42 days, and the material consumption in the test period is recorded and weighed. After the test was completed, broiler productivity indicators were determined (see table 5). On the 21 st day and the 42 th day of the test period, 1 broiler chicken is randomly selected for slaughtering repeatedly, tissues of about 1 cm of the vacant field and the anterior segment of the ileum are collected and put into tissue fixing liquid for 24 hours, then tissue sections are made and HE staining is carried out, the intestinal villus form is observed under a microscope, and the villus length, the crypt depth and the intestinal wall thickness are measured. The empty field and the ileum mucosa were scraped off, and the intestinal mucosa oxidation resistance was measured, and the measurement results are shown in table 6.
TABLE 5 broiler productivity
TABLE 6 intestinal mucosa structure of broiler (21 days old)
The results show that when the magnolol and the honokiol are applied to daily ration of the broilers, the daily gain of the broilers can be obviously increased, the feed conversion ratio is reduced, and the ratio (V/C) of the villus height and the villus height/crypt depth of intestinal tracts of the broilers is greatly improved; by applying the invention, the functions of magnolol and honokiol in increasing the height of intestinal villi and the V/C ratio of the broiler can be further promoted.
Example 3
1200 healthy laying hens, namely 45-week-old Jing powder No. 2, are selected and randomly divided into 4 treatments, and each treatment is 10 times repeated and respectively taken as a control group and a test group. Control group was fed basal diet (ref. Mei)National NRC (2012) layer chicken nutritional requirements, see table 7), test group 1 was fed basal diet +200mg/kg magnolol; test group 2 was fed basal diet +200mg/kg honokiol; test group 3 feeding basic ration and feed additive (comprising 10 parts of magnolol, 10 parts of honokiol, 5 parts of calcium propionate and 5 parts of mannan oligosaccharide, 10 parts of mannan oligosaccharide by weight parts105 parts of CFU/g clostridium butyricum, 5 parts of vitamin E and 60 parts of rice hull powder). The test period is 12 weeks, and is carried out in the hot summer season (6 middle ten months-8 months). The feed consumption, egg production per day and total egg weight were recorded over the test period. Egg quality determinations were performed by randomly selecting 10 eggs per replicate at weeks 4, 8, and 12 of the experiment. After the test is finished, 1 laying hen is selected at random for slaughtering each time, tissues about 1 cm of the vacant field and the front section of the ileum are collected and put into tissue fixing liquid for 24 hours, then tissue sections and HE staining are carried out, the intestinal villus form is observed under a microscope, and the length of the villus, the depth of the crypt and the thickness of the intestinal wall are measured. The empty field and the ileum mucosa are scraped, the oxidation resistance of the intestinal mucosa is measured, and the measurement results are shown in tables 7-10.
TABLE 7 daily ration composition for layers
TABLE 8 egg laying performance of layer chicken
TABLE 9 egg quality
TABLE 10 intestinal mucosa structure and antioxidant ability of laying hens
The results show that the laying rate of the laying hens is obviously reduced in high-temperature seasons, the quality of eggs such as yolk color and eggshell strength is obviously reduced, and the influence of high-temperature stress on the laying performance and the egg quality of the laying hens can be effectively relieved by applying the invention; and can improve intestinal mucosa structure and oxidation resistance of laying hen.
Claims (9)
1. Application of magnolol and/or honokiol in poultry breeding is characterized in that: the magnolol and/or honokiol is applied to the feed additive for promoting intestinal villus development and improving the oxidation resistance of intestinal mucosa.
2. Application of magnolol and/or honokiol in poultry breeding is characterized in that: the magnolol and/or honokiol is applied to the feed additive for relieving intestinal tract pathogenic bacteria infection of poultry, intestinal tract injury caused by stress and production performance reduction.
3. Application of magnolol and/or honokiol in poultry breeding is characterized in that: the magnolol and/or honokiol is applied to the feed additive for reducing the feed conversion ratio of meat to meat or the feed conversion ratio of eggs.
4. Use of magnolol and/or honokiol according to any one of claims 1-3 in poultry farming, characterized in that: the feed additive containing magnolol and honokiol is used for daily ration of broiler chickens, wherein the weight ratio of magnolol to honokiol is 1: 2-3.
5. Use of magnolol and/or honokiol according to any one of claims 1-3 in poultry farming, characterized in that: the feed additive containing magnolol and honokiol is used for daily ration of laying hens, and the weight ratio of magnolol to honokiol is 1: 0.5-1.
6. A magnolol and/or honokiol-containing feed additive according to any one of claims 1 to 3, wherein: the feed additive and the weight portion are 0-30 portions of magnolol, 0-30 portions of honokiol, 5-10 portions of organic acid, 5-10 portions of functional oligosaccharide, 5-10 portions of probiotics, 1-5 portions of vitamin E and 40-75 portions of carrier.
7. The magnolol and/or honokiol-containing feed additive according to claim 6, wherein: the feed additive contains magnolol and/or honokiol total phenol 20-30%.
8. The magnolol and/or honokiol-containing feed additive according to claim 6, wherein: the mass concentration of the feed additive in the feed is 0.5-1 kg/t.
9. The magnolol and/or honokiol-containing feed additive according to claim 6, wherein: the organic acid is any one or more of lactic acid, formic acid, propionic acid, butyric acid, citric acid, fumaric acid and salts thereof;
the functional oligosaccharide is any one or more of mannan oligosaccharide, chitosan oligosaccharide and fructooligosaccharide;
the probiotics are any one or more of bacillus coagulans, bacillus subtilis, clostridium butyricum and lactobacillus;
the carrier is any one of wheat bran, defatted rice bran and rice hull powder.
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111743035A (en) * | 2020-06-24 | 2020-10-09 | 广东海洋大学 | Fish feed additive for improving antioxidant function |
CN112220781A (en) * | 2020-11-17 | 2021-01-15 | 湖北省农业科学院畜牧兽医研究所 | Magnolol and honokiol and method for preventing and treating fatty liver syndrome of laying hens by using magnolol and honokiol |
CN112586607A (en) * | 2020-12-16 | 2021-04-02 | 长沙昆博生物科技有限公司 | Feed additive for improving color of animal carcass and application thereof |
CN113598276A (en) * | 2021-07-29 | 2021-11-05 | 湖北省农业科学院畜牧兽医研究所 | Application of magnolol in improving reproductive performance of sows |
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111743035A (en) * | 2020-06-24 | 2020-10-09 | 广东海洋大学 | Fish feed additive for improving antioxidant function |
CN112220781A (en) * | 2020-11-17 | 2021-01-15 | 湖北省农业科学院畜牧兽医研究所 | Magnolol and honokiol and method for preventing and treating fatty liver syndrome of laying hens by using magnolol and honokiol |
CN112586607A (en) * | 2020-12-16 | 2021-04-02 | 长沙昆博生物科技有限公司 | Feed additive for improving color of animal carcass and application thereof |
CN113598276A (en) * | 2021-07-29 | 2021-11-05 | 湖北省农业科学院畜牧兽医研究所 | Application of magnolol in improving reproductive performance of sows |
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