KR20220070122A - A composition for skin anti-pollution containing a daughter-in-law navel extract - Google Patents

Abstract

The present invention relates to an anti-pollution cosmetic composition comprising an extract of Persicaria perfoliata. The present invention, which comprises natural extracts harmless to the human body, not only can effectively inhibit the apoptosis of skin cells caused by chemicals while alleviating skin irritation, but also can improve the effects of skin moisturizing and skin regeneration.

Description

Composition for skin anti-pollution comprising daughter-in-law's belly button extract {A COMPOSITION FOR SKIN ANTI-POLLUTION CONTAINING A DAUGHTER-IN-LAW NAVEL EXTRACT}

The present invention relates to a composition for skin anti-pollution comprising a daughter-in-law navel extract. More particularly, it relates to a composition comprising a daughter-in-law navel extract having excellent effects of inhibiting apoptosis of skin cells caused by chemicals, as well as excellent skin moisturizing and skin regeneration effects.

Recently, as the quality of life is improved due to the extension of average sleep according to the development of medical technology, the desire for a healthy life is increasing, and at the same time, interest in skin beauty is also increasing.

The skin is a part of the body that is directly exposed to the external environment, and it not only acts as a protective film to protect important organs of our body, but also regulates water evaporation and protects the body from external infections. However, no matter how much the skin blocks the penetration of viruses from the outside, external stress such as excessive UV rays and a polluted environment causes skin irritation, which ultimately leads to skin aging.

As we enter the modern society, environmental pollution is getting worse and the concentration of pollutants around the living area is increasing, so the problem of skin protection from pollutants is emerging as a social problem. Accordingly, various functional cosmetics are being developed, and in particular, investment and research on skin protection cosmetics with an anti-pollution function are being actively conducted.

Examples of these pollutants include formaldehyde, nitrogen oxides, benzopyrene, and the like, and they enter the body and accumulate to cause various diseases.

Specifically, the pollutants cause direct inflammation or necrosis when in contact with the skin at a high concentration, and even if present in a residual amount in the air, continuous contact with it causes continuous stress on the skin, and thus acts as a cause of skin aging.

Compared to the effects of pollutants on the skin, the public's awareness of the risks is still low. number is increasing.

Accordingly, the development of cosmetic compositions, detergents, and external preparations having an anti-pollution effect that prevents the environmental pollutants from adhering to the surface of the skin or penetrating into the body through the skin is being actively developed. However, most of the currently developed products simply remove the contaminants from the skin as well as remove the contaminants that have adhered to or penetrate the skin, and do not fundamentally protect the skin from environmental contaminants. There was a slight problem with the ability to protect the skin from

In particular, since it contains chemicals as a main component, there is a problem that rather acts as a cause of skin troubles and skin diseases. Accordingly, there is a need to develop a composition that is hypoallergenic and has excellent anti-pollution effects on the skin using natural substances.

On the other hand, as using natural substances, in Korean Patent Registration No. 10-1571323, peppermint, chamomile, rosemary, lemongrass, wild chrysanthemum, tea tree and lavender are each mixed in the same weight ratio and extracted by using a herbal complex extract, Disclosed is a cosmetic composition for improving skin having a solution effect, and Korean Patent Laid-Open No. 10-2017-0053388 discloses a cosmetic composition having an anti-pollution effect by containing turmeric, apple, and grape extracts. , In the case of the above patent, there was a problem that the anti-pollution effect was insignificant.

On the other hand, as mentioned above, the skin is in direct contact with the external environment and has a function of protecting our body from various harmful factors, but it is gradually damaged by the increase of age, the influence of ultraviolet rays, external pollutants and stress, so that it protects cells and protects cells. The proliferative ability is reduced, thereby accelerating skin aging.

Specifically, this skin aging phenomenon is mainly due to the cutting and low molecular weight of hyaluronic acid, the sluggishness of collagen production in fibroblasts, etc. It appears due to a decrease in skin elasticity due to the weakening of

In general, the moisture content of the skin is about 70% in the dermis layer, but decreases toward the epidermis layer and becomes about 10 to 30% in the keratinocyte layer. Water supplied from the dermal layer moves to the upper part of the stratum corneum mainly by passive diffusion and is finally discharged to the outside. This is called Trans Epedermal Water Loss (TEWL), and it is known that such transdermal water loss is maintained at an appropriate level by the sebum component and epidermal lipid, which are the lipid membrane components of the keratinocyte layer.

In addition, it is known that a substance having a hydrophilic water retention ability called Natural Moisturizing Factor (NMF) exists in the keratinocyte layer and plays an important role in moisturizing the skin. When the moisture in the keratinocyte layer is maintained at about 10 to 30%, the skin is smooth and soft, and the body's protective function is normally exhibited. However, when the moisture content of the keratinocyte layer is 10% or less, the skin becomes rough and the protective function of the body is lost, and aging occurs.

For example, in the case of dry skin, the cohesion of keratinocytes is weakened, and a scale phenomenon appears in which the keratinocytes are peeled off like scales from the surface of the skin. This is because it is smaller than normal skin. In addition, even with healthy skin, the skin condition may deteriorate due to a lack of moisture due to a harsh external environment, that is, wind, cold weather, sunlight, washing face, or shaving. Therefore, it is most important to prevent skin aging to properly maintain the moisture content of the keratinocyte layer of the skin.

For this purpose, a component similar to sebum, an NMF component, or a moisturizing agent such as polyol has been added and used in cosmetics.

For example, as water-soluble polyols, glycerin and sorbitol having three or more hydroxyl groups (-OH groups) are added, and while these have the advantage of showing excellent moisturizing power, they have a strong stickiness, which makes the skin uncomfortable when used and depends on the relative humidity. I have a problem with taking away my moisture. In addition, when propylene glycol and 1,3-butylene glycol having two hydroxyl groups (-OH groups) are added, side effects on the skin may occur. Other natural moisturizing factors, such as sodium pyrrolidone carboxylate (PCA-Na) and sodium lactate, have a problem of impairing the emulsion stability of cosmetics due to their strong electrolytic properties. It is known that there is, but there is a limit to its moisturizing ability.

Accordingly, the present applicant has developed a composition comprising a daughter-in-law extract, which is a natural extract, as a composition that solves the above problems and has an excellent skin protection effect from chemicals, as well as an excellent skin moisturizing and skin regeneration effect.

KR 10-1571323 B1 KR 10-2017-0053388 A

It is an object of the present invention to provide a composition for skin anti-pollution comprising a daughter-in-law extract, which effectively inhibits apoptosis of skin cells caused by chemicals, including a natural extract that does not cause skin irritation. It is to provide a composition that can not only protect the skin, but also improve the skin moisturizing and skin regeneration effect.

Another object of the present invention is to provide a cosmetic composition for skin anti-pollution comprising a daughter-in-law navel extract as an active ingredient.

Another object of the present invention is to provide a lotion comprising a daughter-in-law navel extract as an active ingredient.

In order to achieve the above object, a composition for skin anti-pollution comprising a daughter-in-law extract according to an embodiment of the present invention contains the daughter-in-law extract as an active ingredient, and the effect of inhibiting the death of skin cells by chemicals is excellent

The chemical is selected from the group consisting of polycyclic aromatic hydrocarbons, aldehydes and nitrogen oxides.

The composition has excellent skin moisturizing and skin regenerating effects.

The composition will further include a first natural extract selected from the group consisting of Dume leek extract, Shigeori tree extract, and mixtures thereof.

The composition further includes other additives, and the other additives are excipients, disintegrants and lubricants.

In the present invention, the extract is extracted using an extraction solvent selected from the group consisting of water, alcohols having 1 to 4 carbon atoms, ether, chloroform, ethyl acetate, dimethyl sulfoxide, and mixtures thereof.

A cosmetic composition for skin anti-pollution according to another embodiment of the present invention includes the composition.

A lotion according to another embodiment of the present invention includes the composition.

Hereinafter, the present invention will be described in more detail.

As used herein, the term 'extract' has the meaning commonly used in the art as a crude extract, but broadly includes a fraction obtained by additionally fractionating the extract. That is, the plant extract includes not only those obtained by using the above-described extraction solvent, but also those obtained by additionally applying a purification process thereto. For example, a fraction obtained by passing the extract through an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (prepared for separation according to size, charge, hydrophobicity or affinity), etc. Fractions obtained through various purification methods are also included in the extract of the present invention.

The extract used in the present invention may be prepared in a powder state by an additional process such as distillation under reduced pressure and freeze-drying or spray-drying.

As used herein, the term "comprising as an active ingredient" refers to an amount sufficient to affect a desired biological effect, such as, but not limited to, beneficial results, including, but not limited to, prevention, reduction, alleviation or elimination of signs or symptoms of a disease or disorder. is meant to include, and this amount is referred to as an “effective amount”. Accordingly, the total amount of each active ingredient of a composition or method is sufficient to exhibit a significant individual benefit. Accordingly, the effective amount will vary depending on the circumstances in which it is administered. An effective amount may be administered in one or more prophylactic or therapeutic administrations.

The composition for skin anti-pollution comprising a daughter-in-law extract according to an embodiment of the present invention contains the daughter-in-law extract as an active ingredient, and is excellent in the effect of inhibiting apoptosis of skin cells by chemicals.

Anti-pollution means 'protects the body from various environmental harmful factors such as fine dust and prevents pollution'. It's a Lusion product.

On the other hand, recently, the problem of environmental pollution is emerging due to the adverse effect of rapid urbanization, and chemical substances contained in various pollutants and fine dust act on the skin, causing various skin diseases. Specifically, when various chemical substances act on the skin, it not only causes the death of skin cells, but also inhibits the proliferation of skin cells, thereby causing inflammation, etc., and develops into various skin diseases.

Throughout the specification of the present invention, "anti-pollution" refers to improving or preventing skin contamination by chemicals, skin cell death, or inhibition of skin cell proliferation.

On the other hand, the daughter-in-law belly button (Persicaria perfoliata) is a kind of the origin of the plant family of Asia. Daughter-in-law's belly button has traditionally been used as an antidote, antipyretic, and diuretic, and has also been used for respiratory diseases such as cough and whooping cough. It is known that quercetin, caffeic acid, etc. are contained as major components of the daughter-in-law's navel extract. The quercetin (quercetin) is a plant antioxidant and belongs to the flavonoid family among phytochemicals. Quercetin plays a role in modulating the immune system's response to external stressors through cellular signaling pathways called kinases and phosphatases, two types of enzymes and membrane proteins required for proper cellular function.

When the daughter-in-law extract is included as a component of the composition, it is possible to effectively inhibit the death of skin cells caused by various chemicals acting on the skin by the action of the active ingredient contained in the daughter-in-law extract, It has the effect of improving skin moisture and skin elasticity.

The chemical is selected from the group consisting of polycyclic aromatic hydrocarbons, aldehydes and nitrogen oxides.

Specifically, the polycyclic aromatic hydrocarbon may include benzopyrene, and the aldehydes may include formaldehyde.

In addition, the nitrogen oxides may include nitrogen oxides represented by the chemical formula of NO _X , more specifically NO, NO ₂ , N ₂ O, N ₄ O, N ₂ O ₃ , N ₂ O ₄ , N ₂ O ₅ , N ₄ O ₆ or an ionide thereof may be included.

The benzopyrene is produced due to incomplete combustion, such as automobile exhaust gas or cigarette smoke, and is a toxic substance that causes cell damage through metabolic conversion to epoxide and reaction with DNA. Recently, as news of inducing atopic dermatitis is reported, adverse effects on the skin are exposed, and it is one of the dangerous substances that can cause skin deterioration due to problems such as cell mutation and cell damage.

The formaldehyde is a colorless gas with a strong irritant odor at room temperature, also called methanal. The formaldehyde is easily produced when a material containing carbon is incompletely burned, and is synthesized in the air by sunlight and oxygen being added to hydrocarbons such as methane. The formaldehyde is one of the skin allergens, and may cause problems such as skin rash, skin necrosis, and dermatitis when in contact with the skin.

The nitrogen oxide is a compound composed of nitrogen and oxygen, and there are ionized products of nitrogen monoxide, nitrogen dioxide, dinitrogen monoxide, dinitrogen trioxide, dinitrogen tetraoxide, dinitrogen pentoxide, and the like. In general, nitrogen gas is stable and it is difficult to split the atoms constituting its composition into two, but when a very high temperature is applied, the bond may be broken. Often, the high temperature is formed inside the engine of the vehicle, so that the exhaust gas of the vehicle contains a large amount of nitrogen oxides.

The nitrogen oxide may include ammonium or a salt thereof. Nitric acid, another form of nitrogen oxide, reacts with water to form nitric acid, which can also cause acid rain. The nitric acid is combined with ammonium, which is an alkaline substance in the atmosphere, to produce ammonium nitrate. The ammonium nitrate is a particulate matter in the atmosphere, which is secondary fine dust and adversely affects the human body. When the ammonium nitrate comes into contact with the human body, it causes inflammation in the eyes, nasal mucosa, and skin.

Specifically, the chemicals may act on the skin to promote the death of skin cells, which causes skin aging.

On the other hand, the composition is excellent in skin moisturizing and skin regeneration effect.

Fundamentally, moisturizing of the skin allows the keratinocytes to maintain a well-arranged structure due to intercellular lipids, and serves as a passageway for physiologically necessary substances to be absorbed, thereby keeping the skin moist and healthy. That is, the lamellar liquid crystal structure formed by the intercellular lipids can prevent moisture loss to the outside and retain moisture in the body, thus performing a function as a skin barrier. At this time, if the function of the cytoplasmic lipids as a skin barrier is impaired, moisture loss is high and moisture content is insufficient, so direct moisture supply is required.

In addition, keratinocytes of healthy people have high concentrations of natural moisturizing factors (NMFs) to help the skin retain moisture. However, in spite of these functions of the skin, the skin is exposed to the harsh external environment, that is, wind, cold weather, sunlight, etc., in the polluted atmosphere along with washing, shaving, and indoor heating in winter, and the aging factor is added with age. Since the skin easily loses moisture and tends to become dry, the need for a skin moisturizer is increasing to prevent this. According to this need, various studies are being conducted to show the effect of moisturizing the skin by increasing water retention in the stratum corneum. On the other hand, recently, it has been newly discovered that, in addition to simply increasing water in the stratum corneum, the differentiation process in which the keratinocytes of the basal layer are developed into keratinocytes of the stratum corneum can be performed normally to serve as a moisture retention barrier for the skin. Accordingly, research is also underway to promote the differentiation of keratinocytes and ultimately improve the moisturizing power of the skin.

On the other hand, among the theories of skin aging, the most popular theory is aging caused by active oxygen. More than 95% of oxygen consumed by the human body is reduced to water by combining with electrons generated in the metabolic process of cells, but 2 to 3% of oxygen is incomplete reduction, causing cell destruction in the free radical process to absorb electrons. This is called active oxygen. Free radicals are generated during the process of respiration and digestion essential to sustain life, as well as various factors that threaten our health, such as smoking, stress, excessive exercise, skin diseases, viral infections, and ultraviolet rays. Therefore, humans are continuously exposed to the threat of free radicals, and therefore our body has various antioxidant mechanisms in response to the damage caused by free radicals to protect cells in various ways.

In particular, free radicals cause damage to skin cells. When there are too many free radicals, the antioxidant defense system in the body is broken down, and as a result, cellular components such as proteins, lipids, and DNA are damaged, and cellular functions are altered, ultimately promoting skin aging. In addition, free radicals damage the connective tissue. Active oxygen not only directly destroys collagen by acting on collagen metabolism, but also causes collagen deficiency, thereby causing skin cell aging.

The daughter-in-law navel extract, which is an active ingredient included in the composition for skin anti-pollution of the present invention, is a natural extract that is harmless to the human body. This can prevent skin aging.

More specifically, the activity of fibroblasts is promoted by the action of the active ingredient of the daughter-in-law's navel extract, thereby not only improving the skin regeneration effect, but also strengthening the skin barrier and increasing the moisture content, thereby improving skin moisturizing power. .

On the other hand, the skin regeneration represents not only skin regeneration, but also wrinkle improvement, skin elasticity improvement, skin aging prevention, skin moisturizing improvement, age spots removal, acne treatment and wound removal effect, and more preferably skin regeneration, wrinkle improvement, It means improving skin elasticity and preventing skin aging.

The composition will further include a first natural extract selected from the group consisting of Dume leek extract, Shigeori tree extract, and mixtures thereof.

The Doume leek (Allium senescens) is a perennial monocotyledonous plant belonging to the Liliaceae, like the commonly known leek, and is mainly distributed in Ulleungdo and northern regions. The scaly stems are egg-shaped and oval, with a thin membranous skin and no fibers. In addition, Dume leek has thick leaves and rich flesh, so it contains a lot of moisture and essential oil. In oriental medicine, it is classified as a warm plant. is known Recently, as it has been found that it contains a large amount of polyphenol, known as an antioxidant, it is expected that it will be able to exhibit excellent efficacy in anti-aging, which is the greatest concern of modern people.

In particular, Dume leek contains a large amount of adenosine and saponin, which prevents the formation of blood clots and purifies the blood, so it is effective in preventing cerebrovascular disease and high blood pressure. In addition, the mucin component of Dume leek aids digestion when ingested, has the effect of protecting the stomach wall, and has a detoxifying effect by discharging cholesterol from the body. Allicin, a component that gives off a bittersweet scent, has antibacterial and anti-inflammatory effects on the body, so it is helpful for various inflammations such as gastritis, enteritis, and bronchitis, and has the effect of releasing toxins accumulated in the liver. However, since it has a warm nature, people with a lot of heat may feel a sense of heat if eaten, and if consumed excessively at once, it may cause abdominal pain and diarrhea.

The Silgeori tree (Caesalpinia decapetala) is a deciduous deciduous shrub belonging to the dicotyledonous family Rosaceae, and is also called a sageori tree and a salgeori tree. It grows in the sun at the foot of the mountain and is widely distributed in Korea, Japan, southern China, and India. It is about 4m in length and has thorns bent downwards. The leaves are alternate phyllotaxis, and there is a reproductive bud in the leaf axil with 2 pinnate compound leaves. Small leaves are 5 to 10 pairs each, oval in shape, and about 1 to 2 cm long. The fruit is a conifer, flat oval, about 9cm long and about 2.7cm wide, and does not spread. Silgeori tree is mainly used as a medicine for headaches or muscle pains in folklore as a fruit.

Preferably, the composition may include 15 to 20 parts by weight of the Dume leek extract and 15 to 20 parts by weight of the Dume leek extract and 15 to 20 parts by weight of the extract, based on 100 parts by weight of the daughter-in-law's navel extract. By more effectively inhibiting cell death, the anti-pollution effect on the skin can be improved, and the skin moisturizing and skin regeneration effect can be further increased.

More preferably, the composition may further include a second natural extract consisting of a mixture of an extract of a rhododendron tree and a pheasant tree extract.

The sweet tree (Raphiolepis indica var. umbellata (Thunb.) Ohashi) is an evergreen shrub that grows at the foot of a mountain along the coast, reaching a height of 2 to 4 m, and its twigs are whorl. The leaves are alternate, but they look like they grow on the tips of branches, are oblong or obovate, and have dull serrations at the edges. Flowers are white and run in panicles from April to June. The fruits are round and ripen black.

The fruit of the sweet berry has the effect of dilating blood vessels and improving blood flow, so it is used for heart diseases such as irregular heartbeat, high blood pressure, and chest pain.

The pheasant tree (Viburnum erosum Thunb.) is a deciduous broad-leaved shrub belonging to the genus Homophylliaceae, and it is a tree commonly found in low mountains south of Gyeonggi-do. The height is 2-3m, and the white flowers in spring and red fruits in autumn are beautiful, so it is often planted as a park tree. The oval-shaped leaves run opposite to each other, and the hairs on the front and back have fine hairs, giving a soft feeling to the touch. The stem is divided into several and grows to form a leaf. The flowers are gathered in a round shape with white bisexual flowers at the tip of a new branch in April or May. The small red bean-like fruits ripen in September to October, making them good food for birds. It grows in a forest with moderate sunlight, likes a soil with good moisture and drainage, and has very strong cold resistance to withstand the cold and grows well in both the sun and the shade. On the other hand, the name pheasant tree seems to be related to pheasants, and it is presumed that it was called a pheasant tree because it has fruits that pheasants in the field like. Young leaves can be eaten as herbs, and old leaves and stems are used in oriental medicine for stomatitis or itching.

When the composition further includes a second natural extract of the oleander extract and the pheasant tree extract, through the mixing action with the daughter-in-law extract and the first natural extract, it is possible to more effectively inhibit the death of skin cells by chemicals. In addition, it is possible to regenerate the skin by enhancing the cellular activity of fibroblasts and further improve skin elasticity. In addition, it is possible to maximize the skin moisturizing effect by increasing the moisture content in the skin.

More preferably, based on 100 parts by weight of the daughter-in-law's belly button extract, 15 to 20 parts by weight of Dume leek extract, 15 to 20 parts by weight of serrata oleifera extract, 5 to 10 parts by weight of extract of chrysanthemum tree extract, and 5 to 10 parts by weight of pheasant tree extract. can

According to the weight range, it is possible to maximize the effect of inhibiting the apoptosis of the skin cells, as well as to further enhance the skin moisturizing and skin regeneration effect, and when it is less than or exceeding the range value, the effect is significantly There is a problem with falling.

The composition further includes other additives, and the other additives are excipients, disintegrants and lubricants.

The excipient is selected from the group consisting of prosolve, starch, microcrystalline cellulose, mannitol, lactose, low-substituted hydroxypropyl cellulose, sucralose, and mixtures thereof, specifically, Prosolv (Prosolv SMCC90), starch (Starch 1500) ), microcrystalline cellulose (microcrystalline cellulose PH102) and mannitol (mannitol 160C), but is not limited to the above examples.

The disintegrant is selected from the group consisting of crospovidone, sodium starch gluconate, croscarmellose sodium, polysorbate, sodium lyryl sulfate and mixtures thereof, more specifically crospovidone (crospovidone CL-SF), It is not limited to the above example.

The lubricant is selected from the group consisting of magnesium stearate, light anhydrous silicic acid, talc, glyceryl behenate, and mixtures thereof, more specifically magnesium stearate, but is not limited to the above example.

In the present invention, the extract is extracted using an extraction solvent selected from the group consisting of water, alcohols having 1 to 4 carbon atoms, ether, chloroform, ethyl acetate, dimethyl sulfoxide, and mixtures thereof. The 'daughter-in-law navel extract' means an extract obtained by extracting from the daughter-in-law navel.

The daughter-in-law extract is a pulverized product of a dried daughter-in-law's navel, water, ethanol, alcohol having 1 to 4 carbon atoms, such as methanol, a polar solvent such as dimethyl sulfoxide, or a mixture ratio of alcohol and water 1:0.1 to 1:10 It can be eluted with a mixed solvent having At this time, the extraction temperature may be 10 ℃ to 100 ℃, preferably the extract extracted at 25 ℃.

The extraction solvent may be used in an amount of 2 to 50 times, preferably 2 to 20 times, based on the weight of the sample. For extraction, the sample may be left for 1 to 72 hours for leaching in the extraction solvent, and in particular, from 1 to 24 hours.

It may be a result obtained by concentrating the filtered extract under reduced pressure with a vacuum rotary concentrator, but as long as it is a daughter-in-law extract capable of inhibiting skin cell death by the chemical of the present invention and exhibiting skin moisturizing and skin regeneration effects, it is limited thereto It includes all extracts, diluted or concentrated solutions of extracts, dried products obtained by drying the extracts, or prepared or purified products thereof.

The Dume leek extract, sageori tree extract, dandelion tree extract, and pheasant tree extract can also be prepared using the same method as the daughter-in-law's belly button extract.

A cosmetic composition for skin anti-pollution according to another embodiment of the present invention includes the composition.

The cosmetic composition of the present invention may further include ingredients such as water, oil, surfactant, moisturizer, thickener, lower alcohol, colorant, preservative, and fragrance, which are ingredients formulated in general skin cosmetics.

In addition, the cosmetic composition may be prepared in any formulation conventionally prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, It may be formulated as oil, powder foundation, emulsion foundation, wax foundation and spray, but is not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, a nourishing lotion, a nourishing cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.

When the formulation of the cosmetic of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or oxide as a carrier component Zinc or the like may be used.

In addition, when the cosmetic formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluorohydro It may contain a propellant such as carbon, propane/butane or dimethyl ether.

When the formulation of the cosmetic of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate; There are propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan.

When the formulation of the cosmetic of the present invention is a suspension, a liquid diluent such as water, ethane or propylene glycol as a carrier component, ethoxylated isostearyl alcohol, polyoxyethylene sorbitan ester, and polyoxyethylene sorbitan ester, such as As a suspending agent, microcrystalline selulose aluminum metahydroxide vantonite or tracanth may be used.

When the cosmetic formulation of the present invention is surfactant-containing cleansing, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative methyl taurate, sarcocy Nate, fatty acid amide ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative or ethoxylated glycerol fatty acid ester, etc. may be used.

The components included in the cosmetic composition of the present invention include components commonly used in cosmetic compositions in addition to the peptide and carrier components as active ingredients, for example, antioxidants, stabilizers, solubilizers, vitamins, pigments and fragrances. Supplements may be included.

A lotion according to another embodiment of the present invention includes the composition.

According to the composition for skin anti-pollution comprising the daughter-in-law extract of the present invention, it is possible to use a natural extract harmless to the human body to relieve skin irritation and at the same time have an excellent effect of inhibiting apoptosis of skin cells caused by chemicals. In addition, it is possible to provide a composition for skin anti-pollution excellent in skin moisturizing and skin regeneration effect. Furthermore, it is possible to provide a cosmetic composition and lotion having the above effect.

1 is a test result of apoptosis inhibitory effect of a skin anti-pollution composition according to an embodiment of the present invention.
2 is a fibroblast activity test result of the composition for skin anti-pollution according to an embodiment of the present invention.

Hereinafter, embodiments of the present invention will be described in detail so that those of ordinary skill in the art can easily carry out the present invention. However, the present invention may be embodied in several different forms and is not limited to the embodiments described herein.

[Preparation Example 1: Preparation of natural extract]

The daughter-in-law navel leaf was pulverized with a blender and then prepared as a powder. After adding 50% ethanol as an extraction solvent to the powder sample at a ratio of 1:10 (w; v), the powder sample was completely immersed, and extracted three times for 3 hours while refluxing at 80°C. The extract is Whatman No. 2 filtered with filter paper. The filtrate was concentrated under reduced pressure at 60° C. to prepare a daughter-in-law navel extract (PE).

Dume leek extract (AE), sageori tree extract (CE), sweet chrysanthemum extract (RE), and pheasant tree extract (VE) were prepared in the same manner as in the preparation method of the daughter-in-law's belly button extract (PE).

[Preparation Example 2: Preparation of Mixed Composition]

Prosolv SMCC90, starch Starch1500, microcrystalline cellulose PH102, mannitol 160C, and crospovidone CL-SF used as a disintegrant used as the natural extract and excipients were put into a 30 mesh sieve and apple was put into a 30 mesh sieve to prepare a mixture, and the mixture was prepared as a lubricant Magnesium stearate was added and mixed and tableted to prepare a mixed composition.

The content of the natural extract included in the mixed composition is shown in Table 1 below.

T1 T2 T3 T4 T5 T6 T7 T8 T9 T10 T11 T12 T13 T14 T15 PE 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 AE - 17 - 13 15 17 20 22 17 17 17 17 17 17 17 CE - - 17 13 15 17 20 22 17 17 17 17 17 17 17 RE - - - - - - - - 7 - 3 5 7 10 12 VE - - - - - - - - - 7 3 5 7 10 12

(Unit: parts by weight)

[Experimental Example 1: Skin cell death inhibitory effect test]

In general, the degree of cell damage caused by benzopyrene (2.5 ppm) was measured using skin keratinocytes that come in direct contact with pollutants (control group), and T1 to T15 of Preparation Example 2 were treated to benzopyrene (2.5 ppm). The degree of cell damage was measured, and by comparison, the skin cell death inhibitory effect of the composition was confirmed.

Specifically, 200 μl of dermal keratinocytes HaCaT was dispensed into a 96-well plate at a concentration of 1×10 ⁴ cells/ml, and cultured at 37° C., CO ₂ in an incubator for 24 hours. In this case, IMDM medium containing 10% bovine serum and 1% antibiotic was used as the medium.

After culturing, the medium was replaced with an IMDM medium excluding bovine serum and antibiotics, treated with benzopyrene, and then treated with T1 to T15 and further cultured for 21 hours. Thereafter, MTT solution of 5 mg/ml concentration was added and cultured for 3 hours. Then, the medium was removed, and 150 μl of dimethyl sulfoxide was added to each well, followed by shaking for 10 minutes, and absorbance was measured at 570 nm. The results are shown in Table 2 and FIG. 1 below.

Cell death rate (%) Control (benzopyrene only) 60 T1 55 T2 53 T3 56 T4 55 T5 44 T6 40 T7 43 T8 55 T9 54 T10 49 T11 48 T12 35 T13 31 T14 33 T15 49

Looking at Table 2 above, when the composition of T1 to T15 was treated, it was confirmed that there was an effect of inhibiting the apoptosis of skin keratinocytes compared to the control, effectively protecting the skin from chemicals contained in fine dust, etc. It can be seen that it works.

In particular, when the composition of T5 to T7 and the composition of T12 to T14 are treated, it can be seen that the effect of inhibiting the apoptosis of the skin keratinocytes is maximized, depending on the weight range of the natural extract included in the composition. , it can be seen that the anti-pollution effect is the best.

[Experimental Example 2: Skin moisturizing effect test]

In order to test the skin moisturizing effect according to the composition of the present invention, 100 males and females (50 males, 50 females) aged 22 to 33 years who did not have skin diseases that could affect the experimental results were tested. They washed the test site with water before application of each composition and then allowed them to adapt to the external environment for 30 minutes.

Experimental environmental conditions were carried out in the same place maintained at an indoor temperature of 22 to 24 °C and an indoor humidity of 45 to 50%. 20 μL of each of the compositions of T1 to T15 was applied to the cheeks at 2Х2cm ² , and the skin surface conductivity of each application site was measured at 30-minute intervals (0 minutes, 30 minutes, 60 minutes) with a vapometer (Vapometer, delfin company). It is shown in Table 3 below.

For comparison of the moisturizing effect, a 1% aqueous solution of hyaluronic acid, which is known to have an excellent moisturizing effect, was used.

0 minutes 30 minutes 60 minutes Control (no treatment) 7.0 2.8 1.3 T1 6.8 3.5 2.4 T2 7.1 3.4 2.1 T3 7.0 3.0 2.2 T4 6.9 2.9 2.3 T5 7.0 3.8 3.6 T6 6.8 4.0 3.7 T7 6.9 3.9 3.5 T8 7.1 3.3 2.1 T9 7.2 3.0 2.9 T10 6.7 3.1 2.9 T11 7.0 3.0 2.8 T12 7.1 5.5 4.5 T13 7.0 5.6 4.9 T14 6.9 5.2 4.6 T15 7.2 2.9 2.7

(Unit: g/m ² hr)

Looking at Table 3 above, it can be seen that when the composition of T1 to T15 is treated, it exhibits an equal to or more moisturizing effect than that of hyaluronic acid.

In particular, when the composition of T5 to T7 and the composition of T12 to T14 are treated, it can be seen that the moisturizing effect is maximized. Based on the weight range of the natural extract included in the composition, skin barrier strengthening and moisture content It can be confirmed that by increasing the moisture content of the skin, it maximizes the skin's moisturizing power.

[Experimental Example 3: Skin regeneration effect test]

Cell activity was measured using MTT (3-(4,5-dimethylthiazol-2-yl)-2-5-diphenyltetra-zolium bromide) reagent to confirm the skin regeneration promoting effect of the T1 to T15 compositions.

After culturing human-derived fibroblasts and inoculating them in a 96-well plate incubator at a concentration of 1 X 10 ⁵ cells/well, the T1 to T15 compositions were applied for about 24 hours, and then the MTT test was performed to determine the cell activity promoting effect. The skin regeneration effect was confirmed.

Cell activation ability was evaluated by comparing the sample with a diluted vehicle (DMEM containing 1% FBS serum). As the medium of Comparative Example, DMEM containing 1% of FBS serum was used. The results of cell activity were selected according to Equation 1 below, and the test was repeated three times. The results are shown in Table 4 and Figure 2 below.

[Equation 1]

Cell activity (%) = (response absorbance of treatment group - response absorbance of control group/response absorbance of control group) X 100

Cell activity (%) Control (no treatment) 0 T1 15 T2 16 T3 14 T4 19 T5 27 T6 29 T7 28 T8 20 T9 21 T10 22 T11 25 T12 35 T13 38 T14 36 T15 25

Looking at Table 4 above, it can be seen that when the composition of T1 to T15 is treated, it has the effect of regenerating the skin by promoting the cell activity of fibroblasts.

In particular, when the composition of T5 to T7 and the composition of T12 to T14 are treated, it can be seen that the cell activity of fibroblasts is maximized. Based on the weight range of the natural extract contained in the composition, the skin regeneration effect is It can be seen that the best

[Experimental Example 4: Skin irritation test]

50 males and 50 females aged 20 to 40 years old were selected, divided into 5 groups of 20 people each, and after using the lotion containing the T1 to T15 composition, a survey was conducted on whether skin irritation occurred, The results are shown in Table 5 below.

[Evaluation standard]

◎: No irritation at all

○: Almost no irritation

△: There is slight irritation, so it is not recommended to use it on sensitive skin

Х : Impossible to use due to severe irritation

group 1 group 2 group 3 group 4 group 5 T1 ○ ○ △ △ ○ T2 ○ △ ○ △ ○ T3 ○ △ △ ○ ○ T4 ○ ○ △ △ △ T5 ◎ ○ ◎ ○ ◎ T6 ◎ ○ ◎ ○ ◎ T7 ◎ ○ ○ ◎ ◎ T8 ○ △ △ △ ○ T9 ◎ ◎ ○ ○ ○ T10 ○ ○ ◎ ◎ ○ T11 ○ ○ ◎ ○ ○ T12 ◎ ○ ◎ ◎ ◎ T13 ◎ ◎ ◎ ○ ◎ T14 ○ ◎ ◎ ◎ ◎ T15 ○ △ ◎ ○ ○

According to Table 5, the lotion including the composition of T1 to T15 was evaluated as overall not severe skin irritation.

In particular, in the case of lotions containing the compositions of T5 to T7 and T12 to T14, the skin irritation was evaluated to be the least, and based on this, the composition of the present invention uses a natural extract, a component harmless to the human body, for those with sensitive skin. It can also be confirmed that it is effective for

Although preferred embodiments of the present invention have been described in detail above, the scope of the present invention is not limited thereto, and various modifications and improvements by those skilled in the art using the basic concept of the present invention as defined in the following claims are also provided. is within the scope of the

Claims (8)

Contains daughter-in-law's belly button extract as an active ingredient,
Excellent effect in inhibiting apoptosis of skin cells caused by chemicals
A composition for skin anti-pollution comprising a daughter-in-law's navel extract. According to claim 1,
The chemical is
Which is selected from the group consisting of polycyclic aromatic hydrocarbons, aldehydes and nitrogen oxides
A composition for skin anti-pollution comprising a daughter-in-law navel extract. According to claim 1,
The composition has excellent skin moisturizing and skin regeneration effects
A composition for skin anti-pollution comprising a daughter-in-law's navel extract. According to claim 1,
The composition further comprises a first natural extract selected from the group consisting of Dume leek extract, Shigeori tree extract, and mixtures thereof
A composition for skin anti-pollution comprising a daughter-in-law's navel extract. According to claim 1,
The composition further comprises other additives,
The other additives are excipients, disintegrants and lubricants.
A composition for skin anti-pollution comprising a daughter-in-law's navel extract. The method of claim 1,
The extract is extracted using an extraction solvent selected from the group consisting of water, alcohols having 1 to 4 carbon atoms, ether, chloroform, ethyl acetate, dimethyl sulfoxide, and mixtures thereof.
A composition for skin anti-pollution comprising a daughter-in-law navel extract. A cosmetic composition for skin anti-pollution comprising the composition according to any one of claims 1 to 6. A lotion comprising the composition according to any one of claims 1 to 6.

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