KR20220063410A - Cosmetic composition for improving acne of skin comprising glutathionyl genistein - Google Patents

Abstract

The present invention relates to a cosmetic composition for alleviating acne skin and, more specifically, to a cosmetic composition for alleviating acne skin containing glutathionyl genistein as an active component. The cosmetic composition has excellent antibacterial activity against acne-causing bacteria due to the glutathionyl genistein, and it is possible to alleviate acne skin inflammation, thereby exhibiting an excellent alleviation effect on acne skin symptoms.

Description

Cosmetic composition for acne skin improvement containing glutathionyl genistein as an active ingredient {COSMETIC COMPOSITION FOR IMPROVING ACNE OF SKIN COMPRISING GLUTATHIONYL GENISTEIN}

The present invention relates to a cosmetic composition for improving acne skin, and more specifically, by containing glutathionyl genistein as an active ingredient, it has excellent antibacterial action against acne causative bacteria and can alleviate acne skin inflammation. It relates to a cosmetic composition for improving acne prone skin containing glutathionyl genistein as an active ingredient, which exhibits an excellent improvement effect.

The need for beauty is being recognized anew as the importance and demand for skin health care as well as physical health are increasing in pursuit of well-being with the improvement of the living standard that is being developed. This interest in beauty is expanding not only to women but also to men, and the concept of beauty is particularly interested in the act of cultivating healthy skin that can look beautiful without makeup through skin beautification rather than looking beautiful through plastic surgery or makeup. At this time of transition, acne among skin problems is positioned as one of the most common concerns and problems in our lives (Eun-Jeong Shin, Comparison of Acne Knowledge and Lifestyles of Adult Male Acne Patients and Jeong San-in, Asian Beauty Cosmetics Journal, 361-369, 2012).

Although the exact cause of acne is not known, in general, the interaction between several factors such as abnormal hair follicle stagnation, hyperkeratinization, increase in sebum secretion, and proliferation of Propionibacterium acne (P. Although it has been found to be caused by an inflammatory reaction caused by the outflow of substances from the hair follicles into the dermis (Kligman. A.M. Postadolscent acne in women, Cutis. 1991), acne that occurs after puberty is usually caused by stress, stress, and If internal and external triggers such as menstruation, pregnancy, cosmetics, etc. occur, acne will occur at any time. Moreover, it was confirmed early that the longer the duration of acne, the more severe acne scars, and the fact that effective management for adult acne after the age of 25 should be carried out at an early stage gave importance to his research (Laton. AM, A comparison of intralesional triamcinolone and cryosurgery in treatment of acne keloide, 1995; Cunliffe. W. Natural history of acnes in Acne and related disorders, London, Martin Dunitz, 1989).

The treatment drugs for acne known so far must play at least one of these three roles: regulation of sebum secretion, correction of abnormal keratinization process, and inhibition of bacteria represented by propionibacterium acnes. will be. Specifically, to suppress the hypersecretion of sebum, the use of female hormones such as anti-androgens that control the secretion of abnormal male hormones, the use of antibacterial agents and antibiotics that inhibit the growth of acne bacteria, and non-steroidal anti-inflammatory drugs with anti-inflammatory action In the cosmetic field, ingredients such as resorcinol, sulfur, salicylic acid, and benzoyl peroxide are added to exfoliate the skin and inhibit acne bacteria has been mainly used.

However, if these ingredients are added in excess, skin irritation and dry side effects are likely to occur, so there is a limit on the formulation in the case of resorcinol and salicylic acid, and ingredients such as benzoyl peroxide are designated as prohibited ingredients in Korea. .

On the other hand, glutathione (Glutathione) is a representative antioxidant material present in the living body, glutathamic acid (glutathamic acid), cysteine (cysteine) and glycine (glycine), such as a substance consisting of three amino acids. Glutathione exists in a reduced state (GSH) and an oxidized state (GSSG) and plays an important role in almost all redox reactions in vivo. In addition, it is a flavonoid type belonging to the genistein polyphenol class and is generally widely distributed in plants. Genistein is a natural substance with low toxicity and antioxidant function, which is a general characteristic of flavonoid substances.

Techniques related to a cosmetic or therapeutic composition for skin improvement comprising glutathione and genistein having the above-described effects as active ingredients are known. Korean Patent No. 10-1725101 discloses a technology related to a composition for external application for skin whitening containing glutathione, and Korean Patent No. 10-1082819 discloses a soybean fermented extract containing genistein extracted from soybean as an active ingredient. A technology related to a functional composition for alleviating atopic dermatitis containing is disclosed.

However, in the description of the composition capable of preventing acne occurrence and improving acne skin symptoms, both glutathione and genistein are contained as active ingredients or, in particular, according to the polyphenol and cysteinyl thiol reaction method, genistein ( genistein) and glutathione, and the development of a cosmetic composition for improving acne prone skin containing glutathionyl genistein as an active ingredient has not been developed.

Therefore, in the present invention, while research was conducted to find a substance that is excellent in preventing acne and improving acne skin symptoms, glutathionyl genistein prepared by reacting genistein and glutathione according to a polyphenol and cysteine thiol reaction method is used for acne skin symptoms. It was confirmed that the improvement effect was excellent, and a significantly superior effect could be exhibited compared to the case where any one of these components was used alone.

Republic of Korea Patent Registration No. 10-1725101 Republic of Korea Patent Registration No. 10-1082819

The present invention has been devised to solve the problems as described above and provide the necessary technology,

The present invention relates to a cosmetic composition for improving acne skin, and more specifically, by containing glutathionyl genistein as an active ingredient, it has excellent antibacterial action against acne causative bacteria and can alleviate acne skin inflammation. It relates to a cosmetic composition for improving acne prone skin containing glutathionyl genistein as an active ingredient, which exhibits an excellent improvement effect.

As an embodiment of the present invention for achieving the above object,

The present invention provides a cosmetic composition for improving acne prone skin containing glutathionyl genistein as an active ingredient.

In the present invention, the glutathionyl genistein may be 6-glutathionyl genistein or 8-glutathionyl genistein.

In the present invention, the glutathionyl genistein may be characterized in that it is included in a ratio of 0.005 to 1% by weight based on the total weight% of the cosmetic composition.

In the present invention, the cosmetic composition is vitamin E (Vitamin E), vitamin D ((Vitamin D), quercetin (Quercetin), polysorbate 80 (Polysorbate 80), allantoin (Allantoin), glycerin (Glycerin), niacin ( Niacin), adenine (Adenine), cysteine (Cysteine), lavender oil (Lavender oil), and may be characterized in that it further comprises any one or more selected from the group consisting of purified water (Water).

In the present invention, the cosmetic composition comprises a solution, an external ointment, a cream, a foam, a nutritional lotion, a flexible lotion, a pack, an emulsion, a makeup base, an essence, a soap, a liquid detergent, a bath agent, a sunscreen cream, a sun oil, a suspension, an emulsion Liquid, paste, gel, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, it may be characterized in that any one formulation selected from the group consisting of patches and sprays.

The cosmetic composition for improving acne prone skin according to an embodiment of the present invention contains glutathionyl genistein as an active ingredient, thereby having an excellent antibacterial action against acne causative bacteria, alleviating acne skin inflammation, and reducing acne by reducing the number of acne It is very effective in alleviating and preventing symptoms.

That is, the cosmetic composition for improving acne prone skin containing glutathionyl genistein as an active ingredient according to an embodiment of the present invention inhibits the growth of Propionibacterium acnes , an acne-generating bacterium, and exhibits an antibacterial effect, By activating the discharge of sebum and reducing the amount of sebum in the sebaceous glands, it is possible to significantly improve the acne severity index (Michaelson's Acne Severity Index), which is very effective in preventing the occurrence of acne and improving symptoms.

1 is a photograph showing the results of an antibacterial effect experiment of glutathionyl genistein on acne bacteria (KCOM 1466).

Hereinafter, embodiments of the present invention will be described in detail so that those of ordinary skill in the art to which the present invention pertains can easily carry out the present invention. The embodiments of the present invention are provided in order to more completely explain the present invention to those of ordinary skill in the art. Accordingly, the embodiment of the present invention may be modified in various other forms, and the scope of the present invention is not limited to the embodiments described below.

Throughout the specification of the present invention, when a part "includes" a certain component, it does not exclude other components unless otherwise stated, but further includes other components.

The terms "about", "substantially", etc. to the extent used throughout the specification of the present invention are used in or close to the numerical value when manufacturing and material tolerances inherent in the stated meaning are presented, and the present invention It is used to prevent an unconscionable infringer from using the disclosure in which exact or absolute figures are mentioned to help the understanding of the

The present invention provides a cosmetic composition (hereinafter also referred to as 'cosmetic composition') for improving acne skin containing glutathionyl genistein as an active ingredient.

According to one embodiment of the present invention, the glutathionyl genistein may be characterized in that it is included in a ratio of 0.005 to 1% by weight based on the total weight% of the cosmetic composition.

That is, it is preferable that glutathionyl genistein is included in a ratio of 0.005 to 1% by weight with respect to the total weight of the cosmetic, which is not effectively expressed when glutathionyl genistein is included in a ratio of less than 0.005% by weight. This is because, when glutathionyl genistein is included in a ratio exceeding 1% by weight, skin side effects may occur and problems such as solubility in the formulation may occur.

According to an embodiment of the present invention, the glutathionyl genistein may be 6-glutathionyl genistein or 8-glutathionyl genistein.

In the present invention, 6-glutathionyl genistein or 8-glutathionyl genistein prepared by reacting genistein and glutathione according to the polyphenol and cysteinyl thiol reaction method is used. Most preferred.

Although not limited thereto, the cosmetic composition for improving acne prone skin containing glutathionyl genistein as an active ingredient according to an embodiment of the present invention may be used in combination with glutathionyl genistein as it is or with other similarly active ingredients. and may be appropriately used according to a conventional method. The mixing amount of the active ingredient may be appropriately determined according to the purpose of use.

According to an embodiment of the present invention, the cosmetic composition is vitamin E (Vitamin E), vitamin D ((Vitamin D), quercetin (Quercetin), polysorbate 80 (Polysorbate 80), allantoin (Allantoin), glycerin (Glycerin) ), niacin (Niacin), adenine (Adenine), cysteine (Cysteine), lavender oil (Lavender oil) and purified water (Water) may be characterized in that it further comprises any one or more selected from the group consisting of.

That is, the cosmetic composition of the present invention may be formulated by arbitrarily selecting various cosmetically acceptable ingredients according to the formulation or purpose of use, in order to be used as a cosmetic composition for improving acne skin in each formulation. The mixing amount of the active ingredient may be appropriately determined depending on the intended use, and may include, for example, conventional adjuvants and carriers such as thickeners, stabilizers, solubilizers, vitamins, pigments and fragrances.

The cosmetic composition for improving acne prone skin containing glutathionyl genistein as an active ingredient according to the present invention may be used as a cosmetic composition.

According to one embodiment of the present invention, the cosmetic composition is a solution, external ointment, cream, foam, nutritional lotion, flexible lotion, pack, emulsion, makeup base, essence, soap, liquid detergent, bath agent, sunscreen cream, sun oil , suspension, emulsion, paste, gel, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, characterized in that any one formulation selected from the group consisting of patches and sprays can do.

The cosmetic composition for improving acne skin according to the present invention may have a conventional formulation, for example, it may be formulated as a mist, lotion (soft, astringent), nourishing cream, essence (body essence), sun cream, and the like. The composition of each of these formulations may contain various gases and additives necessary and appropriate for the formulation of the formulation, and the types and amounts of these components can be easily selected by the developer.

In addition, the cosmetic composition for improving acne prone skin according to the present invention can be used according to a conventional method of use, and the number of times of use can be varied according to the user's skin condition or taste.

Although not limited thereto, the cosmetic composition for improving acne prone skin containing glutathionyl genistein as an active ingredient according to an embodiment of the present invention may be processed into an external preparation for acne skin improvement.

The external preparation for skin is not particularly limited in its formulation, such as in the form of skin liniments, liquids, powders, capsules, tablets, syrups, etc., and various formulations known in the art to relieve and improve acne skin symptoms. can be used as The external preparation for skin may be formulated by including one or more pharmaceutically acceptable carriers in addition to the active ingredients described above.

In addition, although not limited thereto, the cosmetic composition for improving acne prone skin containing glutathionyl genistein as an active ingredient according to an embodiment of the present invention may be processed into a quasi-drug for improving acne skin.

The quasi-drug is not particularly limited in its formulation, and it can be formulated in various forms in the form of quasi-drugs known in the art to exhibit the alleviation and improvement effect of acne skin symptoms. The formulated product includes shampoo, conditioner, body wash, detergent, detergent soap, disinfectant cleaner, wet tissue, mask, patch or filter filler, and the like, and includes all quasi-drugs in a conventional sense. The quasi-drug may be formulated by including one or more acceptable carriers as a component of the quasi-drug in addition to the active ingredient described above.

Hereinafter, the present invention will be described in detail according to specific examples. After the cosmetic composition for improving acne-prone skin of the present invention prepares a cosmetic lotion, which is a representative cosmetic, this will be described as an example. However, the following examples are merely illustrative of the present invention, and the content of the present invention is not limited to the following examples.

Preparation of Glutathionyl genistein

Genistein and glutathione used in the glutathionyl genistein manufacturing experiment were purchased from Sigma-Aldrich and used.

Glutathionyl genistein is Fujimoto A and Masuda T (Fujimoto A, Masuda T. Chemical interaction between polyphenols and a cysteinyl thiol under radical oxidation conditions. J Agric Food Chem. 2012 23;60(20):5142-51 ) was prepared by reacting genistein and glutathione according to the polyphenol and cysteinyl thiol reaction method of H1) NMR) analysis method confirmed the structures of 6-glutathionyl genistein (6-Glutathionyl genistein) and 8-glutathionyl genistein (8-Glutathionyl genistein). The structures of 6-glutathionyl genistein and 8-glutathionyl genistein are as follows.

[Formula]

Measurement of the Minimum Growth Inhibitory Concentration (MIC) of Glutathionyl genistein for acne bacteria

The antibacterial activity of glutathionyl genistein against acne bacteria was observed by measuring the minimum growth inhibitory concentration.

In order to measure the minimum growth inhibitory concentration of glutathionyl genistein against acne bacteria, the minimum growth inhibitory concentrations for 6-glutathionyl genistein and 8-glutathionyl genistein were respectively measured, and to compare the related effects. The minimum growth inhibitory concentrations for glutathione, genistein and a simple mixture of glutathione and genistein were also measured and compared.

Propionibacterium acnes ( P. acnes ; KCOM 1466) used in this experiment was purchased from the Korean Collection for Oral Microbiology (KCOM), Gwangju, Korea) and used, and glutathionyl genistein was carried out above. The one prepared in Example 1 was used.

The minimum inhibitory concentration was measured by the broth dilution method (Murray & Jorgensen, (1981) Antimicrob Agents Chemother. 20:66-70). That is, after culturing the bacteria for 24 hours at 37°C in a bacterial incubator using a selective medium, dilute it to 1×10 ⁶ CFU/ml and dispense it in a cell culture vessel (96-well plate), glutathionyl genistein and genistein Silver was dissolved in ethanol at a concentration of 50 mg/ml, respectively, and then added to the bacterial culture, and sequentially diluted two-fold so that the final concentrations were 0.5, 0.25, 0.125, 0.065, 0.0325 and 0.01625 mg/ml. In addition, glutathione was dissolved in distilled water at a concentration of 50 mg/ml and then added to the bacterial culture medium, and the final concentrations were 0.5, 0.25, 0.125, 0.065, 0.0325, and 0.01625 mg/ml, and sequentially diluted two-fold. In addition, a simple mixture of glutathione and genistein was added to the bacterial culture medium in a simple mixing state so that the mole ratio of glutathione (molecular weight: 307.32) and genistein (molecular weight: 270.4) was 1:1, and the final concentration was 0.5, 0.25 , 0.125, 0.065, 0.0325 and 0.01625 mg/ml were sequentially diluted two-fold.

Ethanol was used for the negative control group of the experiment, and the antibiotic tetracycline was used for the positive control group. The minimum inhibitory concentration was observed by measuring the absorbance at 600 nm after culturing for 24 hours for the bacterial culture solution dispensed in a cell culture vessel (96-well plate), and the results are shown in Table 1 below.

matter P.acnes (KCOM 1466)
Minimum growth inhibitory concentration (mg/ml) glutathione >0.5 Genistein 0.25 A simple mixture of glutathione and genistein 0.5 6-Glutathionyl Genistein 0.125 8-Glutathionyl Genistein 0.125 ethanol >0.5 tetracycline <0.01625

As shown in Table 1, the minimum growth inhibitory concentration of ethanol as a control group for acnes bacteria ( P. acnes, KCOM 1466) was measured to be 0.5 mg/ml or more, so the concentration of ethanol used in the experiment was for acne bacteria. It was confirmed that there was no antibacterial activity, and tetracycline, a positive control, was confirmed to have an antibacterial activity with a minimum growth inhibitory concentration of 0.01625 mg/ml or less against Acne acnes ( P. acnes, KCOM 1466).

Acne ( P. acnes, KCOM 1466), the minimum growth inhibitory concentration of glutathione was 0.5 mg/ml or more, and the minimum growth inhibitory concentration of genistein was measured to be 0.25 mg/ml. Therefore, the concentration of glutathione used in this experiment was It was confirmed that there was no antibacterial activity against In addition, as the minimum growth inhibitory concentration of the simple mixture of glutathione and genistein was measured to be 0.5 mg/ml, it was confirmed that the antibacterial activity was lower than that of genistein.

On the other hand, the minimum growth inhibitory concentration of 6-glutathionyl genistein was measured to be 0.125 mg/ml, and the minimum growth inhibitory concentration of 8-glutathionyl genistein was measured to be 0.125 mg/ml, so the antibacterial action against acne bacteria was high. The minimum growth inhibitory concentration of 6-glutathionyl genistein and 8-glutathionyl genistein was measured to be 50% of the minimum growth inhibitory concentration of genistein, confirming that the antibacterial activity was superior to that of genistein.

Antibacterial effect of glutathionyl genistein against acne bacteria

acnes ( P. acnes ) strain KCOM 1466 on blood-agar medium (Blood-Agar medium: tryptic soy broth 3%, yeast extract 0.5%, 0.025% resazurin 2㎖, agar 1.5%, cysteine HCl-H ₂ O 0.05%, Hemin solution 0.5㎎/㎖, vitamin K ₁ 2㎍/㎖, sheep blood 5.0%) (BRL) and anaerobic gas mixture (anaerobic gas mixture: 85% N ₂ , 10% H ₂ using an anaerobic bacterial incubator (Bactron 300)) , 5% CO ₂ ).

6-glutathionyl genistein 0.07 mg/ml, 8-glutathionyl genistein 0.07 mg/ml, genistein 0.07 mg/ml, glutathione 0.07 mg/ml and a simple mixture of glutathione and genistein (glutathione 0.037 mg/ml) on blood agar medium + Genistein 0.033 mg/ml) was added to 0.07 mg/ml, and after culturing for 3 days, the number of bacterial colonies was measured and observed, and the results are shown in Table 2 and FIG. 1 below.

experimental group Additives and dosage number of bacterial colonies negative control - 800 or more Genistein Genistein (0.07 mg/ml) 173 glutathione group Glutathione (0.07 mg/ml) 800 or more Glutathione and Genistein Simple Mixture Glutathione (0.037 mg/ml) +
Genistein (0.033mg/ml) 729 glutathionyl
Genistein 6-glutathionyl genistein (0.07 mg/ml) 52 8-glutathionyl genistein (0.07 mg/ml) 21 positive control Tetracycline (0.005 mg/ml) 0

As shown in Table 2 and FIG. 1, as a result of observing the number of bacterial colonies after culturing acne bacteria strain (P.acnes , KCOM 1466) on blood agar medium for 3 days, genistein, glutathione and glutathionyl genistein were added. In the control group without it, the number of bacterial colonies was 800 or more, and the glutathione (0.07 mg/ml) group also showed more than 800 bacterial colonies, confirming that the control group and glutathione group had no antibacterial activity.

In contrast, the genistein (0.07 mg/ml) group had 173 bacterial colonies, and the 6-glutathionyl genistein (0.07 mg/ml) group and 8-glutathionyl genistein (0.07 mg/ml) group had the bacterial colonies, respectively. As indicated by 52 and 21, it can be confirmed that 6-glutathionyl genistein and 8-glutathionyl genistein have antibacterial activity against acne bacteria, and the number of bacterial colonies in the glutathionyl genistein group is in the genistein (0.07 mg/ml) group. It was confirmed that the antibacterial activity of glutathionyl genistein was more excellent.

In addition, as the number of bacterial colonies in the simple mixture of glutathione and genistein (glutathione 0.037 mg/ml + genistein 0.033 mg/ml) group was 729, it was confirmed that the antibacterial activity against acne bacteria was very low. It can be seen that the simple mixture of genistein has very low antibacterial activity against acne bacteria compared to 6-glutathionyl genistein or 8-glutathionyl genistein.

Preparation of a cosmetic composition containing glutathionyl genistein

According to an embodiment of the present invention, lotions of experimental groups 1-1 and 1-2 were prepared using a cosmetic composition for improving acne prone skin containing glutathionyl genistein as an active ingredient, and did not contain glutathionyl genistein as an active ingredient. The lotions of Comparative Groups 1-1 to 1-4 using the cosmetic composition for improving acne-prone skin were prepared.

1. Preparation of lotion of experimental group 1-1

6-glutathionyl genistein 0.07% by weight, vitamin E 0.01% by weight, vitamin D 0.005% by weight, quercetin 0.01% by weight, polysorbate 80 1.0% by weight, allantoin 1.0% by weight, glycerin 2.0% by weight, niacin 0.02% by weight, Adenine 0.01% by weight, cysteine 0.05% by weight, lavender oil 0.01% by weight and purified water were added and adjusted to 100% by weight, and then mixed to prepare a cosmetic composition for improving acne skin of Experimental Group 1-1, a lotion.

In Table 3 below, (a) phase was mixed and stirred at 70 ° C. for 30 minutes with a stirrer, and then phase (b) was added, emulsified uniformly for 30 minutes using a homomixer, and gradually cooled to room temperature to obtain the results of Experimental Group 1-1. A lotion (cosmetics composition) was prepared.

2. Preparation of lotion in experimental group 1-2

A cosmetic composition for improving acne skin of Experimental Group 1-2 was prepared in the same manner as the lotion of Experimental Group 1-1, but containing 8-glutathionyl genistein as an active ingredient.

3. Preparation of lotion of Comparative Group 1-1

Cosmetic composition for acne skin improvement of Comparative Group 1-1, which was prepared in the same manner as the lotion of Experimental Group 1-1, but did not contain all of glutathionyl genistein, glutathione, genistein, and a simple mixture of glutathione and genistein as active ingredients Phosphorus lotion was prepared.

4. Preparation of lotion of Comparative Group 1-2

A lotion, which is a cosmetic composition for improving acne skin of Comparative Group 1-2, was prepared in the same manner as the lotion of Experimental Group 1-1, but containing glutathione as an active ingredient instead of glutathionyl genistein.

5. Preparation of lotion of Comparative Groups 1-3

A cosmetic composition for improving acne skin of Comparative Group 1-3 was prepared in the same manner as the lotion of Experimental Group 1-1, but containing genistein as an active ingredient instead of glutathionyl genistein.

6. Preparation of lotion of Comparative Groups 1-4

A lotion, which was prepared in the same manner as the lotion of Experimental Group 1-1, but containing a simple mixture of glutathione and genistein as an active ingredient instead of glutathionyl genistein as an active ingredient, was a cosmetic composition for improving acne prone skin of Comparative Group 1-4.

ingredient
(Unit: wt%) control group
1-1 control group
1-2 control group
1-3 control group
1-4 experimental group
1-1 experimental group
1-2 go glutathione - 0.07 - 0.037 - - Genistein - - 0.07 0.033 - - 6-glutathionyl
Genistein - - - - 0.07 - 8-glutathionyl
Genistein - - - - - 0.07 vitamin E 0.01 0.01 0.01 0.01 0.01 0.01 vitamin D 0.005 0.005 0.005 0.005 0.005 0.005 quercetin 0.01 0.01 0.01 0.01 0.01 0.01 Polysorbate 80 1.00 1.00 1.00 1.00 1.00 1.00 allantoin 1.00 1.00 1.00 1.00 1.00 1.00 glycerin 2.00 2.00 2.00 2.00 2.00 2.00 me niacin 0.02 0.02 0.02 0.02 0.02 0.02 adenine 0.01 0.01 0.01 0.01 0.01 0.01 cysteine 0.05 0.05 0.05 0.05 0.05 0.05 lavender oil 0.01 0.01 0.01 0.01 0.01 0.01 Purified water to 100 to 100 to 100 to 100 to 100 to 100

The effect of improving acne skin symptoms of a cosmetic composition containing glutathionyl genistein

In order to observe the improvement of acne skin symptoms of the cosmetic composition for improving acne prone skin containing glutathionyl genistein as an active ingredient, the lotion of Experimental Groups 1-1 and 1-2 prepared in Example 4 and Comparative Group 1-1 To 1-4 lotions were applied to people with acne-prone skin, and the effect of improving skin symptoms was observed.

Acne skin symptom improvement effect test was conducted for 4 weeks on volunteer participants with acne skin on their face We followed the ethical regulations of the Bioethics Committee. There were 30 participants in the experiment, and the age distribution was between 19 and 35 years old.

After explaining the contents and method of the experiment in detail to the participants in the experiment, 6 groups of 5 each It was divided into comparative groups 1-4) and carried out for 4 weeks.

The lotion of Experimental Groups 1-1 and 1-2 and the lotion of Comparative Groups 1-1 to 1-4 prepared in Example 4 were supplied to each experimental group. The supplied lotion was applied to the acne-prone skin once every morning and evening for 4 weeks in the same way in all the experimental groups, and the skin was photographed using a facial imaging device once a week. Before skin imaging, the skin was washed for cleanliness and rested for 30 minutes, and then the acne target area was photographed using a facial imaging device (A-ONE Lite; Ver.2.01, Bomtech Electronics Co., Ltd., Korea). The number of acne was measured in the photographed photos, and the severity of acne was calculated by the Michaelson's Acne Severity Index (ASI) by Equation 1 below.

[Formula 1]

ASI = (Number of comedones×0.5) + (Number of papules×1) + (Number of pustules×2) + (Number of nodules×3)

Using the severity of acne measured in each experimental group, the improvement rate of the severity of acne was calculated by Equation 2 below.

[Formula 2]

Table 4 below shows the results of analyzing the improvement rate of acne Middle East in each experimental group.

experimental group Acne moderate (ASI) improvement rate (%) 2 weeks 4 weeks medium Standard Deviation medium Standard Deviation The lotion use group of Comparative Group 1-1 6.7 2.1 7.2 2.5 The lotion use group of Comparative Group 1-2 8.3 3.5 9.7 3.1 The lotion use group of Comparative Groups 1-3 25.1 7.8 19.6 9.4 The lotion use group of Comparative Groups 1-4 12.3 5.3 17.7 7.6 The lotion use group of experimental group 1-1 47.2 9.5 73.6 9.7 The lotion use group of experimental group 1-2 51.5 8.6 72.7 10.2

As shown in Table 4, the improvement rate of moderate acne did not occur in 2 weeks and 4 weeks after application of the lotion (control lotion) of Comparative Group 1-1 in the test participants, and the lotion of Comparative Group 1-2 (containing glutathione) Toner) application test participants also showed no change in the improvement rate of moderate acne after 2 weeks and 4 weeks.

Compared to the lotion in Comparative Group 1-1, the improvement rate of moderate acne after 2 weeks and 4 weeks was 25.1% at 2 weeks and 19.6% at 4 weeks in the participants of the comparative group 1-3 application of lotion (genistein-containing lotion). It was confirmed that the improvement rate of moderate acne was increased, but compared to the lotion in experimental group 1-1 and experimental group 1-2 using glutathionyl genistein lotion, the improvement in acne moderate severity was lower than that of the lotion in the experimental group. Confirmed.

After 2 weeks and 4 weeks after application of the lotion (lotion containing a simple mixture of glutathione and genistein) of Comparative Group 1-4, the improvement rate of moderate acne was 12.3% at 2 weeks and 17.7% at 4 weeks, Comparison group 1-1 Although the improvement rate of moderate acne was increased compared to the lotion of confirmed to be

After 2 weeks and 4 weeks after application of the lotion (6-glutathionyl genistein-containing lotion) in experimental group 1-1, the improvement rate of moderate acne was 47.2% at 2 weeks and 73.6% at 4 weeks. Compared to that, it was confirmed that the 6-glutathionyl genistein-containing lotion was excellent in improving acne skin symptoms as the improvement rate of moderate acne was increased.

In addition, the improvement rate of moderate acne after 2 weeks and 4 weeks after applying the lotion (8-glutathionyl genistein-containing lotion) in experimental group 1-2 was 51.5% at 2 weeks and 72.7% at 4 weeks. It was confirmed that the 8-glutathionyl genistein-containing lotion was also excellent in improving acne skin symptoms as the improvement rate of moderate acne was increased compared to the lotion of -1.

In particular, compared to the lotion of Comparative Group 1-4 containing a simple mixture of glutathione and genistein, the lotion of Experimental Group 1-1 containing 6-glutathionyl genistein and the lotion of Experimental Group 1-2 containing 8-glutathionyl genistein were more prone to acne. It was confirmed that the effect of improving skin symptoms was much better.

Therefore, according to the experimental results observing the improvement of acne skin symptoms of the cosmetic composition according to the content of glutathionyl genistein, 6-glutathionyl genistein or 8 -Cosmeceutical composition containing glutathionyl genistein has excellent antibacterial action against acne-causing bacteria, can relieve acne skin inflammation, and reduce the number of acne to relieve and prevent acne symptoms.

Preparation of a cosmetic composition according to the content of glutathionyl genistein

According to an embodiment of the present invention, a cosmetic composition for improving acne prone skin containing glutathionyl genistein as an active ingredient is prepared, but 6-glutathionyl genistein is titrated to suit the content of glutathionyl genistein defined in the present invention. The lotion of experimental groups 2-1 to 2-3 was prepared by adding it in a ratio, and the content of 6-glutathionyl genistein was set to be lower than the content of glutathionyl genistein defined in the present invention, and comparison group 2-1 The lotion of Comparative Group 2-2 was prepared by setting the content of 6-glutathionyl genistein to be higher than the content of glutathionyl genistein defined in the present invention. The ingredients and content of ingredients included in the lotion of Experimental Groups 2-1 to 2-3 and the lotion of Comparative Groups 2-1 and 2-2 are shown in Table 5 below.

ingredient
(Unit: wt%) control group
2-1 experimental group
2-1 experimental group
2-2 experimental group
2-3 control group
2-2 go 6-glutathionyl
Genistein 0.001 0.005 0.07 1.0 2.0 vitamin E 0.01 0.01 0.01 0.01 0.01 vitamin D 0.005 0.005 0.005 0.005 0.005 quercetin 0.01 0.01 0.01 0.01 0.01 Polysorbate 80 1.00 1.00 1.00 1.00 1.00 allantoin 1.00 1.00 1.00 1.00 1.00 glycerin 2.00 2.00 2.00 2.00 2.00 me niacin 0.02 0.02 0.02 0.02 0.02 adenine 0.01 0.01 0.01 0.01 0.01 cysteine 0.05 0.05 0.05 0.05 0.05 lavender oil 0.01 0.01 0.01 0.01 0.01 Purified water to 100 to 100 to 100 to 100 to 100

According to an embodiment of the present invention, a cosmetic composition for improving acne prone skin containing glutathionyl genistein as an active ingredient is prepared, but 8-glutathionyl genistein is titrated to suit the content of glutathionyl genistein defined in the present invention. The lotion of experimental groups 2-4 to 2-6 was prepared by adding it in a ratio, and the content of 8-glutathionyl genistein was set to be lower than the content of glutathionyl genistein defined in the present invention, and comparison group 2-3 The lotion of Comparative Group 2-4 was prepared by setting the content of 8-glutathionyl genistein to be higher than the content of glutathionyl genistein defined in the present invention. The ingredients and content of ingredients included in the lotion of Experimental Groups 2-4 to 2-6 and the lotion of Comparative Groups 2-3 and 2-4 are shown in Table 6 below.

ingredient
(Unit: wt%) control group
2-3 experimental group
2-4 experimental group
2-5 experimental group
2-6 control group
2-4 go 8-glutathionyl
Genistein 0.001 0.005 0.07 1.0 2.0 vitamin E 0.01 0.01 0.01 0.01 0.01 vitamin D 0.005 0.005 0.005 0.005 0.005 quercetin 0.01 0.01 0.01 0.01 0.01 Polysorbate 80 1.00 1.00 1.00 1.00 1.00 allantoin 1.00 1.00 1.00 1.00 1.00 glycerin 2.00 2.00 2.00 2.00 2.00 me niacin 0.02 0.02 0.02 0.02 0.02 adenine 0.01 0.01 0.01 0.01 0.01 cysteine 0.05 0.05 0.05 0.05 0.05 lavender oil 0.01 0.01 0.01 0.01 0.01 Purified water to 100 to 100 to 100 to 100 to 100

In Tables 5 and 6, phase (a) was mixed and stirred at 70° C. for 30 minutes with a stirrer, then phase (b) was added, emulsified uniformly for 30 minutes using a homomixer, and gradually cooled to room temperature to form an experimental group and A lotion (cosmetics composition) of the comparative group was prepared.

Improvement of acne skin symptoms of cosmetic composition according to glutathionyl genistein content

In order to observe the improvement of acne skin symptoms of the cosmetic composition for improving acne skin according to the content of glutathionyl genistein, the lotion of Experimental Groups 2-1 to 2-6 prepared in Example 6 and Comparative Groups 2-1 to 2 The skin symptom improvement effect was observed by applying the lotion of -4 to people with acne-prone skin.

Acne skin symptom improvement effect test was conducted for 4 weeks on volunteer participants with acne skin on their face We followed the ethical regulations of the Bioethics Committee. There were 50 participants in the experiment, and the age distribution was between 19 and 35 years old.

After explaining the contents and method of the experiment in detail to the participants in the experiment, 10 groups of 5 each 6, Comparative Group 2-1, Comparative Group 2-2, Comparative Group 2-3, and Comparative Group 2-4) were carried out for 4 weeks.

The lotion of Experimental Groups 2-1 to 2-6 and the lotion of Comparative Groups 2-1 to 2-4 prepared in Example 6 were supplied to each experimental group. The supplied lotion was applied to the acne-prone skin once every morning and evening for 4 weeks in the same way in all experimental groups, and the skin was photographed using a facial imaging device once a week. Before skin imaging, the skin was washed for cleanliness and rested for 30 minutes, and then the acne target area was photographed using a facial imaging device (A-ONE Lite; Ver.2.01, Bomtech Electronics Co., Ltd., Korea). The number of acne was measured in the photographed photos, and the severity of acne was calculated by calculating Michaelson's Acne Severity Index (ASI) by Equation 1 of Example 5 above. In addition, using the severity of acne measured in each experimental group, the improvement rate of the severity of acne was calculated by Equation 2 of Example 5 above.

The results of the analysis of the improvement rate of acne Middle East degree of each experimental group are shown in Tables 7 and 8 below.

experimental group Acne moderate (ASI) improvement rate (%) 2 weeks 4 weeks medium Standard Deviation medium Standard Deviation The lotion use group of Comparative Group 2-1 27.4 6.5 34.9 7.2 The lotion use group of experimental group 2-1 41.5 8.4 62.7 9.1 The lotion use group of experimental group 2-2 47.2 9.5 73.6 9.7 The lotion use group of experimental group 2-3 39.7 7.9 59.1 10.5 The lotion use group of Comparative Group 2-2 31.1 4.8 33.2 9.4

As shown in Table 7, the lotion of experimental groups 2-1 to 2-3 prepared by adding 6-glutathionyl genistein in an appropriate ratio had an effect of improving acne skin symptoms.

After 2 weeks and 4 weeks after application of the lotion (lotion containing 0.005 wt% of 6-glutathionyl genistein) in experimental group 2-1, the improvement rate of moderate acne was 41.5% at 2 weeks and 62.7% at 4 weeks, and those of experimental group 2-2 After 2 weeks and 4 weeks after application of lotion (lotion containing 6-glutathionyl genistein 0.07 wt%), the improvement rate of moderate acne was 47.2% at 2 weeks and 73.6% at 4 weeks, and the lotion (6-gluten) in experimental group 2-3 6-glutathionyl based on the total weight of the cosmetic composition, as the rate of improvement of moderate acne after 2 weeks and 4 weeks after 2 weeks and 4 weeks of application (lotion containing 1% by weight of tathionyl genistein) was shown to be 39.7% at 2 weeks and 59.1 7% at 4 weeks. It was confirmed that the lotion containing 0.005 to 1% by weight of genistein had an effect of improving acne skin symptoms.

On the other hand, 2 weeks and 4 weeks in the test participants applying the lotion (lotion containing 0.001 wt% 6-glutathionyl genistein) of Comparative Group 2-1 prepared by adding 6-glutathionyl genistein to a value lower than the appropriate ratio. As the improvement rate of moderate acne after lapse was 27.4% at 2 weeks and 34.9% at 4 weeks, it was confirmed that the improvement rate of moderate acne was lower than that of lotions in experimental groups 2-1 to 2-3.

In addition, 2 and 4 weeks elapsed in the test participants applying the lotion (lotion containing 2 wt% of 6-glutathionyl genistein) of Comparative Group 2-2 prepared by adding 6-glutathionyl genistein to a level higher than the appropriate ratio. As the post-acne moderate improvement rate was 31.1% at 2 weeks and 33.2% at 4 weeks, it was confirmed that the improvement rate of moderate acne was very low compared to the lotions in Comparative Group 2-1 or Experimental Groups 2-1 to 2-3.

experimental group Acne moderate (ASI) improvement rate (%) 2 weeks 4 weeks medium Standard Deviation medium Standard Deviation The lotion use group of Comparative Group 2-3 29.7 9.7 34.2 5.4 The lotion use group of experimental group 2-4 44.6 9.1 65.1 11.4 The lotion use group of experimental group 2-5 51.5 8.6 72.7 10.2 The lotion use group of experimental group 2-6 42.1 9.7 58.1 10.3 The lotion use group of Comparative Groups 2-4 31.1 10.1 32.7 5.4

As shown in Table 8, the lotion of experimental groups 2-4 to 2-6 prepared by adding 8-glutathionyl genistein in an appropriate ratio was shown to have an effect of improving acne skin symptoms.

After 2 weeks and 4 weeks after application of the lotion (lotion containing 8-glutathionyl genistein 0.005 wt%) in experimental group 2-4, the improvement rate of moderate acne was 44.6% at 2 weeks and 65.1% at 4 weeks, After 2 weeks and 4 weeks after application of lotion (lotion containing 8-glutathionyl genistein 0.07 wt%), the improvement rate of moderate acne was 51.5% at 2 weeks and 72.7% at 4 weeks, and the lotion (8-glue) of experimental group 2-6 Toner containing 1% by weight of tathionyl genistein) After 2 weeks and 4 weeks, the improvement rate of moderate acne in the experimenter was 42.1% at 2 weeks and 58.1% at 4 weeks. This lotion containing 0.005 to 1% by weight was confirmed to have an effect of improving acne skin symptoms.

On the other hand, 2 and 4 weeks in the test participants applying the lotion (lotion containing 0.001 wt% of 8-glutathionyl genistein) of Comparative Group 2-3 prepared by adding 8-glutathionyl genistein to a value lower than the appropriate ratio. As the improvement rate of moderate acne after lapse was 29.7% at 2 weeks and 34.2% at 4 weeks, it was confirmed that the improvement rate of moderate acne was lower than that of the lotions in experimental groups 2-4 to 2-6.

In addition, 2 and 4 weeks elapsed in the test participants applying the lotion (lotion containing 2 wt% of 8-glutathionyl genistein) of Comparative Group 2-4 prepared by adding 8-glutathionyl genistein to a level higher than the appropriate ratio. As the post-acne moderate improvement rate was 31.1% at 2 weeks and 32.7% at 4 weeks, it was confirmed that the improvement rate of moderate acne was very low compared to the lotion in Comparative Group 2-3 or Experimental Groups 2-4 to 2-6.

Therefore, according to the experimental results observing the improvement of acne skin symptoms of the cosmetic composition according to the content of glutathionyl genistein, 6-glutathionyl genistein or 8-glutathionyl genistein was 0.005 with respect to the total weight of cosmetics. When the content of glutathionyl genistein is specifically limited to be included in a ratio of 1% by weight to 1% by weight, the cosmetic composition (lotion) according to an embodiment of the present invention has excellent antibacterial action against acne causative bacteria, and acne skin inflammation It has been shown to be effective in alleviating and preventing acne symptoms by reducing the number of acne.

In conclusion, through Examples 1 to 7, the cosmetic composition for improving acne prone skin according to an embodiment of the present invention contains glutathionyl genistein as an active ingredient, thereby causing acne, Propionibacterium acnes ( Propionibacterium acnes ), it has an antibacterial effect, and it activates the discharge of sebum to reduce the amount of sebum in the sebaceous glands, thereby reducing the number of acne, preventing the occurrence of acne and improving symptoms. Confirmed.

Above, the present invention has been described in detail with reference to examples, but the present invention is not limited to the above examples, and can be modified in various forms, and those of ordinary skill in the art within the technical spirit of the present invention It is clear that many variations are possible by the ruler. In addition, within the scope that does not depart from the technical spirit of the present invention described in the claims, various types of substitution, modification and change will be possible by those skilled in the art, and it is also said that it falls within the scope of the present invention. something to do.

Claims (6)

A cosmetic composition for improving acne prone skin containing glutathionyl genistein as an active ingredient. The method according to claim 1,
The glutathionyl genistein is,
A cosmetic composition for improving acne prone skin, characterized in that it is 6-glutathionyl genistein or 8-glutathionyl genistein. The method according to claim 1,
The glutathionyl genistein is,
A cosmetic composition for improving acne skin, characterized in that it is included in a ratio of 0.005 to 1% by weight based on the total weight% of the cosmetic composition. 4. The method according to any one of claims 1 to 3,
The cosmetic composition,
Vitamin E, Vitamin D, Quercetin, Polysorbate 80, Allantoin, Glycerin, Niacin, Adenine, Cysteine (Cysteine), lavender oil (Lavender oil) and purified water (Water) cosmetic composition for improving acne skin, characterized in that it further comprises any one or more selected from the group consisting of additives. 4. The method according to any one of claims 1 to 3,
The cosmetic composition,
solution, ointment, cream, foam, nourishing lotion, flexible lotion, pack, emulsion, makeup base, essence, soap, liquid detergent, bath product, sunscreen cream, sun oil, suspension, emulsion, paste, gel, lotion, powder , soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, a cosmetic composition for improving acne skin, characterized in that it is any one formulation selected from the group consisting of patches and sprays. 5. The method according to claim 4,
The cosmetic composition,
solution, ointment, cream, foam, nourishing lotion, flexible lotion, pack, emulsion, makeup base, essence, soap, liquid detergent, bath product, sunscreen cream, sun oil, suspension, emulsion, paste, gel, lotion, powder , soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, a cosmetic composition for improving acne skin, characterized in that it is any one formulation selected from the group consisting of patches and sprays.

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