KR20090071529A - Cosmetic composition comprising vitamin k1 or vitamin k3 for anti-bacterial and anti-fungal bio-activity - Google Patents

Abstract

A cosmetic composition containing vitamin K1 or K3 for improving skin diseases cure by antibacterial and antifungal activities is provided to suppress microorganism proliferation and have excellent inhibition activity of dandruff bacteria and Trichophyton mentagrophytes. A cosmetic composition having suppression activity of microorganism proliferation comprises 0.001-5.000 weight% of vitamin K1 (phylloquinone) or vitamin K3 (menadione) as an active ingredients. A cosmetic composition for improving the cure of skin diseases, such as acne, tinea pedis, and dandruff comprises 0.001-5.000 weight% of vitamin K1 or vitamin K3 as an active ingredient. The cosmetic composition is used in a form of skin toner, astringent, lotion, nutrition cream, massage cream, essence, gel, pack, powder, suspension, emulsion, spray, hair shampoo, hair treatment, hair tonic, body cleaner, or shower gel.

Description

Cosmetic composition having antibacterial and antifungal activity comprising vitamin K1 or vitamin K3 as an active ingredient {Cosmetic composition comprising Vitamin K1 or Vitamin K3 for anti-bacterial and anti-fungal bio-activity}

The present invention relates to a novel use of vitamin K-based compounds, and more particularly to a cosmetic composition for improving skin diseases by antibacterial and antifungal activity of vitamin K.

Vitamin K is a fat-soluble vitamin and is well known as a fat-soluble compound present in the leaves of plants. A lack of vitamin K in the body leads to vitamin K deficiency, which increases the time it takes for the blood to clot. In 1939, vitamin K was isolated and structurally analyzed in its pure form, and several related compounds with the same activity as vitamin K were also isolated and synthesized. Vitamin K1, a compound discovered in 1939, is synthesized in plants, while vitamin K2 is produced in organs of microorganisms and is an important type in mammalian tissue. And vitamin K3 is made synthetically. Other forms of vitamin K are all converted to vitamin K2 in the body. There are many types of compounds that are closely related to vitamin K2, and besides natural compounds, many synthetic compounds with vitamin K activity (vitamins K4, K5, K6, K7) are used as medicines to reduce the time it takes to coagulate blood. .

In our body, skin reaches 16% of body volume and is easily damaged by strong ultraviolet rays and external pollutants and stress. If not done properly, the skin's immunity is reduced, increasing the exposure to external pollutants and losing elasticity, causing aging. Normal healthy skin has a protective system that protects the skin from these external damaging factors, but due to persistent external stimuli, these protective systems are broken down, and thus the skin loses protection and cell protection and proliferation are poor. . In addition, the reduced moisturizing power and reduced elasticity of the skin promotes skin aging. As a result, the dermis becomes difficult to maintain the moisture of the skin, such as roughness and wrinkles of the skin. In addition, the skin to protect the human body may be present in the skin flora causing skin diseases. The most common skin diseases caused by mycorrhizal fungi are skin diseases such as acne, athlete's foot and dandruff.

Acne varies from person to person, and a combination of several factors, such as the action of male hormones (androgens) that stimulate the sebaceous glands, or hormones called progestones during women's menstrual periods, genetic predisposition, fatigue, stress, etc. It is known to cause disease. The main causes related to the development of acne include the excessive sebum production of testosterone, a male hormone, the formation of myocytes by the closure of pores, and the acne bacterium Propionibacterium acne ( propionibacterium). The onset of action by acnes ) is persuasively accepted by the academic community.

Athlete's causes are Epidermophyton floccosum , a fungus that is a type of fungus, and Microsporum microsporum . audouinii ), Trichophyton ferrugineum , Trichophyton mentagrophytes mentagrophytes ), Trichophyton It is an infection caused by ruburm ), and occurs mainly in a humid environment of the human body such as toenails, toes, nails, palms, and groin.

In addition, the dandruff causing the dandruff is Malassezia globosa ) or the yeast fungus Pityrosporum ovale ), which persists in seborrheic areas such as the scalp of men and women after puberty in which male hormones are released. Normal people account for 46% of the normal bacteria in the seborrheic region. These dandruff bacteria grow excessively due to environmental factors such as climate, sweat, food, and physiological factors such as stress, and exceed 74% of normal bacteria. If it is abnormally high, dandruff occurs, and if it is higher than 83%, seborrheic dermatitis may develop. In addition, the dandruff bacteria are active in sebaceous glands, the secretion of sebaceous glands increases, and inflammation and dandruff often occur, the scalp becomes red, swelling around the hair follicles, itching, and touching the scalp, pain. This inflammation can adversely affect hair follicles and cause hair loss. In addition, dandruff mixes with sebum from the sebaceous glands and becomes bored, which can block pores and cause hair root dystrophy and atrophy, causing hair to fall out.

As a dermatological treatment method such as acne, athlete's foot, dandruff, which is a skin disease caused by the above-mentioned skin flora, a method of inhibiting the growth of skin flora by antibacterial and antifungal agents is mainly used. However, various chemical components used to suppress the growth of these flora may cause side effects in the human body, so care must be taken in the treatment for skin diseases.

As a general treatment for acne, surgical treatment such as scrim removal and the use of cosmetics using antibacterial and antibiotic antibiotics of retinoic acid, benzoyl peroxide and tetracyclin are mainly used. Surgical treatment, however, has a problem that skin care should be continuously performed after the procedure, and antimicrobial and antibiotic methods are known to cause skin irritation and are highly likely to cause side effects such as phototoxicity. In order to treat skin diseases such as athlete's foot or dandruff, oral antifungal agents such as ketoconazole or amphotericine B, or steroids such as crowegen, dermogen, ecoron-G, and elfason are used orally. Or it is used as an external preparation for the skin, and in the case of oral use, there is a problem in that a long-term treatment period is required because the efficacy / effect is slow by taking 7 days, taking 7 days off, and taking 7 days again to reduce toxic damage to the human body. These drugs are highly likely to cause resistant bacteria by long-term treatment, especially those with reduced liver function may have side effects such as stomach discomfort, vomiting, diarrhea and hair loss symptoms. Problems include irritant dermatitis and secondary bacterial infections.

The present inventors have focused on the fact that it is possible to improve skin diseases by inhibiting the proliferation of skin flora causing such skin diseases. As a result, it has been newly confirmed that vitamin K-based compounds have antimicrobial and antifungal activity, and cosmetic compositions containing these ingredients as active ingredients can be used safely on the skin and at the same time effectively improve skin diseases such as acne, athlete's foot and dandruff. It was confirmed that the present invention was completed.

An object of the present invention is to search for a novel physiological activity of a vitamin K-based compound having antibacterial and antifungal activity and apply it to a cosmetic, thereby providing a cosmetic composition containing a vitamin K-based compound having excellent stability and safety, and at the same time, acne, It is to provide a cosmetic composition that effectively contributes to the improvement of skin diseases such as athlete's foot and dandruff. Accordingly, the present invention relates to a cosmetic composition having antibacterial and antifungal activity as an active ingredient of a vitamin K-based compound, and can be used as a substitute for conventional preservatives used in the manufacture of conventional cosmetics, and is a microorganism that causes skin problems. , Athlete's foot, dandruff, etc. has a proliferation inhibitory activity, there is an advantage that can be applied to cosmetics for improving skin diseases. In addition, the microbial growth inhibitory activity has a high activity at a low concentration, the microbial growth inhibitory activity was evaluated very excellent, and the cosmetic composition for improving the skin disease by excellent stability over time in skin safety and harsh conditions It can be usefully used.

As a result of long-term search for natural products and compounds in order to overcome the dermatological problems described above, the present inventors have confirmed that vitamin K-based compounds are active against various harmful bacteria and fungi, particularly skin diseases such as acne, athlete's foot, and dandruff. It has been found to exhibit excellent efficacy in inhibiting the proliferation of microorganisms that cause the disease, and has no side effects and high safety.

The present inventors have continuously performed research mujomgyun, acne bacteria and bideumgyun a tree second piton menta him pie Massachusetts (Trichophyton mentagrophytes ), Propionibacterium acnes ), and Malassezia globosa ) and Pityrosporum ovale , a type of yeast, have excellent antifungal and antimicrobial activity, and have been found to improve side effects and problems of existing acne, athlete's foot and dandruff treatments. The invention was completed. In addition, the present invention was completed by discovering that the above-mentioned vitamin K-based compounds have superior inhibitory activity to the test microorganisms defined as harmful microorganisms and can be used as a substitute for preservatives or preservatives that used conventional parabens and phenolic compounds. Was done.

In order to achieve the above object, with vitamin K1 (phylloquinone) and vitamin K3 (menadione) ① Escherichia coli ( Escherichia) coli ), Pseudomonas aeruginosa ), Staphylococcus aureus , Candida albicans ), Black grain ( Aspergillus) niger ), ② Propionibacterium , a skin fungus acnes ), the athlete's foot fungus Epidermophyton floccosum ), Microsporum audouinii ), Trichophyton ferrugineum ), Trichophyton mentagrophytes ), Trichophyton ruburm), and the bideumgyun do three Asian fungus (Malassezia globosa ), Pityrosporum ovale ), and enteric bacteria ( Enteric) bacilli ) to determine the antimicrobial and antifungal activity by measuring the minimum inhibitory concentration (MIC), respectively. In addition, cosmetics containing vitamin K-based compounds were prepared to verify the antiseptic ability and the minimum inhibitory concentration of microorganisms in each cosmetic composition to verify the reproducibility of antimicrobial and antifungal activity in the cosmetic composition. The safety test verified the stability and safety of the cosmetic composition.

Hereinafter, the configuration and effects of the present invention have been described in detail through formulation and test examples, but the content of the present invention is not limited thereto.

[ Formulation example  1] Vitamin K series Cosmetics  Preparation of the composition

The present inventors prepared a cosmetic containing a vitamin K-based compound as an active ingredient as shown in Table 1, Table 2, Table 3.

Solubilized Formulation Raw material Comparative Example 1-1 Comparative Example 1-2 Formulation Example 1-1 Formulation Example 1-2 Formulation Example 1-3 Formulation Example 1-4 Formulation Example 1-5 Formulation Example 1-6 Formulation Example 1-7 Formulation Example 1-8 Vitamin K1 - - 0.01 0.02 0.04 0.08 - - - - Vitamin K3 - - - - - - 0.01 0.02 0.04 0.08 Butylene Glycol 6.00 6.00 6.00 6.00 6.00 6.00 6.00 6.00 6.00 6.00 Allantoin 0.20 0.20 0.20 0.20 0.20 0.20 0.20 0.20 0.20 0.20 Dipotassium glycylizate 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 Panthenol 0.50 0.50 0.50 0.50 0.50 0.50 0.50 0.50 0.50 0.50 glycerin 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 Sodium hyaluronate 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 Fiji-60 Hydrogenated Castor Oil 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 Citrix Acid 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 Triethanolamine 0.12 0.12 0.12 0.12 0.12 0.12 0.12 0.12 0.12 0.12 ethanol 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 Methylparaben 0.20 - - - - - - - - - Phenoxyethanol 0.20 - - - - - - - - - Spices 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 Purified water Remaining amount Remaining amount Remaining amount Remaining amount Remaining amount Remaining amount Remaining amount Remaining amount Remaining amount Remaining amount

Emulsion formulations Raw material Comparative Example 2-1 Comparative Example 2-2 Formulation Example 2-1 Formulation Example 2-2 Formulation Example 2-3 Formulation Example 2-4 Formulation Example 2-5 Formulation Example 2-6 Formulation Example 2-7 Formulation Example 2-8 Vitamin K1 - - 0.01 0.02 0.04 0.08 - - - - Vitamin K3 - - - - - - 0.01 0.02 0.04 0.08 Cetearyl Alcohol 1.50 1.50 1.50 1.50 1.50 1.50 1.50 1.50 1.50 1.50 Caprylic / Capric Triglycerides 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 Butylene Glycol Dicaprylate / Dicaprate 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 Jojoba Seed Oil 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 Macadamia Seed Oil 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 Petrolatum 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 C14-22 Alcohol 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 C12-20 alkylglucoside 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 Tocopheryl Acetate 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 Dimethicone 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 Butylene Glycol 6.00 6.00 6.00 6.00 6.00 6.00 6.00 6.00 6.00 6.00 glycerin 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 Allantoin 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 Dipotassium glycylizate 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 Sodium hyaluronate 7.00 7.00 7.00 7.00 7.00 7.00 7.00 7.00 7.00 7.00 Panthenol 0.20 0.20 0.20 0.20 0.20 0.20 0.20 0.20 0.20 0.20 Sodium carbomer 0.50 0.50 0.50 0.50 0.50 0.50 0.50 0.50 0.50 0.50 Propylparaben 0.10 - - - - - - - - - Eichparaben 0.20 - - - - - - - - - Methylparaben 0.20 - - - - - - - - - Phenoxyethanol 0.20 - - - - - - - - - Spices 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 Purified water Remaining amount Remaining amount Remaining amount Remaining amount Remaining amount Remaining amount Remaining amount Remaining amount Remaining amount Remaining amount

Shampoo formulation Raw material Comparative Example 3-1 Comparative Example 3-2 Formulation Example 3-1 Formulation Example 3-2 Formulation Example 3-3 Formulation Example 3-4 Formulation Example 3-5 Formulation Example 3-6 Formulation Example 3-7 Formulation Example 3-8 Vitamin K1 - - 0.01 0.02 0.04 0.08 - - - - Vitamin K3 - - - - - - 0.01 0.02 0.04 0.08 Ammonium Lauryl Sulfate 36.00 36.00 36.00 36.00 36.00 36.00 36.00 36.00 36.00 36.00 Sodium laureth sulfate 12.00 12.00 12.00 12.00 12.00 12.00 12.00 12.00 12.00 12.00 Cocamide Diei 2.90 2.90 2.90 2.90 2.90 2.90 2.90 2.90 2.90 2.90 Stearalconium chloride 2.70 2.70 2.70 2.70 2.70 2.70 2.70 2.70 2.70 2.70 Lauramine oxide 2.50 2.50 2.50 2.50 2.50 2.50 2.50 2.50 2.50 2.50 Tocopheryl Acetate 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 menthol 0.50 0.50 0.50 0.50 0.50 0.50 0.50 0.50 0.50 0.50 Panthenylhydroxypropyl steadimonium chloride 0.50 0.50 0.50 0.50 0.50 0.50 0.50 0.50 0.50 0.50 Polyquaternium-7 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 Panthenol 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 Allantoin 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 Propylene glycol 2.50 2.50 2.50 2.50 2.50 2.50 2.50 2.50 2.50 2.50 Niacinamide 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 Pyridoxine HCL 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 Retinyl palmitate 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 Rosemary Leaf Oil 0.30 0.30 0.30 0.30 0.30 0.30 0.30 0.30 0.30 0.30 Methylparaben 0.20 - - - - - - - - - Phenoxyethanol 0.20 - - - - - - - - - Spices 0.20 0.20 0.20 0.20 0.20 0.20 0.20 0.20 0.20 0.20 Purified water Remaining amount Remaining amount Remaining amount Remaining amount Remaining amount Remaining amount Remaining amount Remaining amount Remaining amount Remaining amount

The antimicrobial and antifungal cosmetic composition containing a vitamin K-based compound according to the present invention was prepared according to a conventional cosmetic composition preparation method by mixing the components of Tables 1, 2 and 3. In Tables 1, 2, and 3, Comparative Examples 1-1, 2-1, and 3-1 were added with conventional preservatives used in cosmetic preparation, and Comparative Examples 1-2, 2-2, and 3-2 were added with preservatives. Did not do it. And each formulation example was added to the vitamin K-based compounds by the corresponding concentrations without the usual preservatives used in the manufacture of cosmetics to prepare a cosmetic composition.

The oil phase component content of the cosmetic composition is selected in consideration of the reinforcement of the emulsification ability and economics, etc., and oils that can be mainly used as the oil phase component may include one or more selected from vegetable oils, mineral oils, silicone oils, and synthetic oils. In addition, 0.1 to 5% by weight of a surfactant, a higher alcohol, etc. may be added to reinforce the emulsifying ability. As such surfactants, conventional surfactants such as nonionic surfactants, phospholipids, and the like may be used. As the higher alcohols, alcohols having 12 to 20 carbon atoms may be used alone or in combination of one or more thereof.

In addition, in the cosmetic composition, the aqueous phase component may further add 0.001 to 5% by weight of one or more thickeners such as carbomer, xanthan gum, bentonite and the like to adjust the viscosity or hardness of the aqueous phase. In addition, to the composition of the present invention, if necessary, active ingredients such as higher fatty acids and vitamins, and ingredients used in conventional cosmetics such as sunscreens, antioxidants, antiseptics, fragrances, colorants, and pH adjusters may be further added.

The cosmetic composition of the present invention can be applied as a product absorbed through the skin or a cosmetic product for the purpose of washing the skin or hair, and it is a flexible cosmetic (skin toner), astringent cosmetic, nutrient cosmetic (lotion), nourishing cream, massage It can be prepared in the form of conventional cosmetics such as creams, essences, gels, packs, powders, external ointments, patches, suspensions, emulsions, sprays, hair shampoos, hair treatments, hair tonics, body cleansers, body lotions or essences. And various conventional carriers and additives suitable for each of these formulations and well known in the art.

Table 1, 2, and 3 of the cosmetic formulation examples are presented in order to facilitate the understanding of the present invention, comparative examples and formulation examples. However, the above Comparative Examples and Formulation Examples are merely provided to more easily understand the present invention, and the contents of the present invention are not limited thereto.

[ Test Example  1] Antibacterial and Antifungal Effects of Vitamin K-based Compounds

In order to confirm the antimicrobial and antifungal activity of vitamin K-based compounds, MIC (minimum inhibitory concentration) that inhibits the growth of each species was investigated. Sterilized liquid medium suitable for the cultivation of each bacteria is placed in a 96-well plate or test tube, and each of the subcultured strains is artificially inoculated at 1 × 10 ⁴ to 1 × 10 ⁶ CFU / mL, and then the vitamin K-based compound is fixed. Systematic dilution to concentration was added to the inoculated medium. The inoculated bacteria solution was incubated for about 3 to 5 days under anaerobic or aerobic conditions suitable for the growth of each bacteria, and the concentration of MIC was examined to determine whether the bacteria were killed. Tetracline, an antibiotic component, or ketoconazole, an antifungal component, was used as a comparison group, and the MIC results of the strains of the components are shown in Table 4.

As a result, the vitamin K-based compounds had similar or slightly higher MIC values than tetracycline and ketoconazole, which are antibiotics used in the comparison group. It was confirmed that there is a proliferation inhibitory activity against. This means that vitamin K-based compounds have antimicrobial and antifungal effects on various microorganisms, and mean that the proliferation inhibitory effect on various microorganisms is excellent.

MIC (minimum inhibitory concentration) of vitamin K family compounds against microorganisms Microbe (bacteria) MIC (μg / mL) Remarks tetracycline ketoconazole Vitamin K1 Vitamin K3 Escherichia coli coli ) 0.5 - 8.0 4.0 Pseudomonas aeruginosa ) 0.5 - 2.0 4.0 Staphylococcus aureus ) 1.0 - 2.0 2.0 Candida albicans ) 1.0 - 32.0 64.0 Black grain bacteria ( Aspergillus niger ) 0.5 - 8.0 4.0 Acne Bacteria ( Propionibacterium acnes ) 2.0 - 2.0 2.0 Epidermophyton floccosum ) - 4.0 16.0 32.0 Forum Forum Microsporum audouinii ) - 4.0 32.0 16.0 Trichophyton ferrugineum ) - 4.0 32.0 16.0 Trichophyton mentagrophytes ) - 4.0 16.0 16.0 Trichophyton ruburm ) - 8.0 16.0 8.0 Malassezia globosa ) - 2.0 8.0 4.0 Pityrosporum Oval ovale ) 2.0 - 8.0 16.0 Enterococci ( Enteric bacilli ) 2.0 - 8.0 4.0

[ Test Example  2] Contains vitamin K series Cosmetics  Antibacterial and Antifungal Effects of the Composition

In order to verify whether the antimicrobial and antifungal activity of the vitamin K-based compounds identified in Test Example 1 is reproduced in the cosmetics, the antimicrobial and antifungal effects of the cosmetic formulations prepared in Tables 1, 2 and 3 were investigated. Experiment of Test Example 2 was carried out in accordance with the microbial antiseptic test of the USP XXIV method and CTFA Methods M-3 and M-4. Comparative Examples and Formulation Examples Prepared in Tables 1, 2 and 3 Each 80 g of each cultured strains were artificially inoculated at 1 × 10 ⁵ to 1 × 10 ⁷ CFU / g and then left at room temperature. Inoculation strain is Escherichia coli ), Pseudomonas aeruginosa ), Staphylococcus aureus ), Candida albicans ), Aspergillus niger , Propionibacterium acnes ), Epidermophyton floccosum , Microsporum audouinii , Trichophyton ferrugineum ), Trichophyton mentagrophytes ), Trichophyton ruburm , Malassezia globosa ), Pityrosporum ovale , Enterococci ( Enteric) bacilli ) were inoculated into bacteria and fungi. Then, the comparative examples and formulations inoculated with the strains stored at room temperature were applied to the solid medium by taking 1g each for 28 days at 7 days intervals, and then checking the proliferation of each microorganism and determining the number of the microorganisms to inhibit the growth of the microorganisms. It was confirmed. The results are shown in Table 5 below.

Inhibitory Effect of Cosmetic Composition on Microbial Growth Cosmetic composition Microbial cell count (CFU / g) Remarks Inoculation day 7 days later 14 days later 21 days later 28 days later Comparative Example 1-1 2.3 × 10 ⁷ 0 0 0 0 bacteria 1.0 × 10 ⁵ 0 0 0 0 mold Comparative Example 1-2 2.3 × 10 ⁷ TNTC TNTC TNTC TNTC bacteria 1.0 × 10 ⁵ TNTC TNTC TNTC TNTC mold Formulation Example 1-1 2.3 × 10 ⁷ 2.3 × 10 ⁶ 2.1 × 10 ² 0 0 bacteria 1.0 × 10 ⁵ 6.5 × 10 ⁴ 100 0 0 mold Formulation Example 1-2 2.3 × 10 ⁷ 4.7 × 10 ⁵ 0 0 0 bacteria 1.0 × 10 ⁵ 1.5 × 10 ³ 0 0 0 mold Formulation Example 1-3 2.3 × 10 ⁷ 4.8 × 10 ² 0 0 0 bacteria 1.0 × 10 ⁵ 0 0 0 0 mold Formulation Example 1-4 2.3 × 10 ⁷ 0 0 0 0 bacteria 1.0 × 10 ⁵ 0 0 0 0 mold Formulation Example 1-5 2.3 × 10 ⁷ 4.5 × 10 ⁶ 2.3 × 10 ² 0 0 bacteria 1.0 × 10 ⁵ 3.7 × 10 ⁴ 53 0 0 mold Formulation Example 1-6 2.3 × 10 ⁷ 2.9 × 10 ⁵ 45 0 0 bacteria 1.0 × 10 ⁵ 1.9 × 10 ⁴ 0 0 0 mold Formulation Example 1-7 2.3 × 10 ⁷ 4.8 × 10 ³ 0 0 0 bacteria 1.0 × 10 ⁵ 0 0 0 0 mold Formulation Example 1-8 2.3 × 10 ⁷ 0 0 0 0 bacteria 1.0 × 10 ⁵ 0 0 0 0 mold Comparative Example 2-1 2.3 × 10 ⁷ 0 0 0 0 bacteria 1.0 × 10 ⁵ 0 0 0 0 mold Comparative Example 2-2 2.3 × 10 ⁷ TNTC TNTC TNTC TNTC bacteria 1.0 × 10 ⁵ TNTC TNTC TNTC TNTC mold Formulation Example 2-1 2.3 × 10 ⁷ 1.0 × 10 ⁶ 3.1 × 10 ² 0 0 bacteria 1.0 × 10 ⁵ 7.0 × 10 ⁴ 60 0 0 mold Formulation Example 2-2 2.3 × 10 ⁷ 8.9 × 10 ⁵ 20 0 0 bacteria 1.0 × 10 ⁵ 2.4 × 10 ⁴ 0 0 0 mold Formulation Example 2-3 2.3 × 10 ⁷ 3.7 × 10 ³ 0 0 0 bacteria 1.0 × 10 ⁵ 0 0 0 0 mold Formulation Example 2-4 2.3 × 10 ⁷ 0 0 0 0 bacteria 1.0 × 10 ⁵ 0 0 0 0 mold Formulation Example 2-5 2.3 × 10 ⁷ 5.5 × 10 ⁶ 1.6 × 10 ² 0 0 bacteria 1.0 × 10 ⁵ 6.3 × 10 ⁴ 1.1 × 10 ² 0 0 mold Formulation Example 2-6 2.3 × 10 ⁷ 7.4 × 10 ⁵ 1.0 × 10 ² 0 0 bacteria 1.0 × 10 ⁵ 1.7 × 10 ⁴ 0 0 0 mold Formulation Example 2-7 2.3 × 10 ⁷ 1.3 × 10 ³ 0 0 0 bacteria 1.0 × 10 ⁵ 0 0 0 0 mold Formulation Example 3-8 2.3 × 10 ⁷ 0 0 0 0 bacteria 1.0 × 10 ⁵ 0 0 0 0 mold Comparative Example 3-1 2.3 × 10 ⁷ 0 0 0 0 bacteria 1.0 × 10 ⁵ 0 0 0 0 mold Comparative Example 3-2 2.3 × 10 ⁷ TNTC TNTC TNTC TNTC bacteria 1.0 × 10 ⁵ TNTC TNTC TNTC TNTC mold Formulation Example 3-1 2.3 × 10 ⁷ 3.5 × 10 ⁶ 1.7 × 10 ² 0 0 bacteria 1.0 × 10 ⁵ 3.8 × 10 ⁴ 154 0 0 mold Formulation Example 3-2 2.3 × 10 ⁷ 5.7 × 10 ⁵ 37 0 0 bacteria 1.0 × 10 ⁵ 1.9 × 10 ⁴ 0 0 0 mold Formulation Example 3-3 2.3 × 10 ⁷ 8.7 × 10 ³ 0 0 0 bacteria 1.0 × 10 ⁵ 0 0 0 0 mold Formulation Example 3-4 2.3 × 10 ⁷ 0 0 0 0 bacteria 1.0 × 10 ⁵ 0 0 0 0 mold Formulation Example 3-5 2.3 × 10 ⁷ 1.6 × 10 ⁶ 3.3 × 10 ² 0 0 bacteria 1.0 × 10 ⁵ 7.3 × 10 ⁴ 253 0 0 mold Formulation Example 3-6 2.3 × 10 ⁷ 7.8 × 10 ⁵ 33 0 0 bacteria 1.0 × 10 ⁵ 2.1 × 10 ⁴ 0 0 0 mold Formulation Example 3-7 2.3 × 10 ⁷ 3.2 × 10 ³ 0 0 0 bacteria 1.0 × 10 ⁵ 0 0 0 0 mold Formulation Example 3-8 2.3 × 10 ⁷ 0 0 0 0 bacteria 1.0 × 10 ⁵ 0 0 0 0 mold

Note) TNTC: Too numerous to count (measured by too many cells)

As shown in Table 5, Comparative Examples 1-2, 2-2, and 2-3 without using a preservative had no effect of inhibiting the growth of microorganisms and were unable to measure because the number of cells was too large. In the case of Comparative Examples 1-1, 2-1 and 3-1 to which was added, it was confirmed that all bacteria inoculated after one week were killed. In addition, the antimicrobial growth inhibitory effect of the formulations added with vitamin K-based compound showed a concentration-dependent microbial growth inhibitory effect depending on the amount of the vitamin K-based compound added, and even after 28 days in the formulation containing 0.01 wt% All bacteria were found to die. And it has been confirmed that the same or similar microbial growth inhibitory effect as the existing preservatives in the formulation with a vitamin K-based compound added at a concentration of 0.08% by weight or more. This means that the microbial growth inhibitory effect of Test Example 1 is reproduced in the cosmetic composition formulation, it was confirmed that as the addition amount of the vitamin K-based compound increases the microbial growth inhibition effect also increases in proportion to the concentration. In addition, the formulations containing more than 0.08% by weight of the vitamin K-based compound showed the same or similar microbial growth inhibitory effect as the existing preservative, but it was confirmed that the microbial growth inhibitory effect continued even at the concentration below.

From the results of Test Example 1 and Test Example 2, vitamin K series compounds are excellent in inhibiting the growth of microorganisms against bacteria and fungi, and mean that they have an effect / effect that can replace the existing preservatives. In addition, acne and dandruff bacteria present on the skin has excellent growth inhibitory activity, which means that it can help to improve skin diseases.

[ Test Example  3] Cosmetics  Safety and Stability Test of the Composition

In order to determine the degree of skin irritation with respect to the cosmetic composition formulation example using the vitamin K-based compound according to the present invention, the following experiment was performed. Ten subjects were subjected to a patch test of the upper arm using Haye's Test Chamber. However, those who have experience with psoriasis, eczema, acne, and other skin allergies or who are pregnant, nursing mothers, or contraceptives and antihistamines are excluded from this experiment. The experiment site was wiped with alcohol and dried, and Formulation Examples 1-4, 1-8, 2-4, 2-8, 3-4, and 3 with the highest content of vitamin K among the cosmetic compositions prepared in Formulation Examples -8 was added dropwise to the chamber (15 μg each), and then fixed on the upper arm, which is an experimental site. The patch time was applied for 24 hours, the patch was removed, the test sites were marked with a marking pen, and the test sites were observed after 24 hours, respectively. The results are shown in Table 6.

Skin urethral test result Formulation example Subject patch test result (skin irritation score) Remarks Subject 1 Subject 2 Subject 3 Subject 4 Subject 5 Subject 6 Subject 7 Subject 8 Subject 9 Subject 10 Formulation Example 1-4 0 0 0 0 0 0 0 0 0 0 Formulation Example 1-8 0 0 0 0 0 0 0 0 0 0 Formulation Example 2-4 0 0 0 0 0 0 0 0 0 0 Formulation Example 2-8 0 0 0 0 0 0 0 0 0 0 Formulation Example 3-4 0 0 0 0 0 0 0 0 0 0 Formulation Example 3-8 0 0 0 0 0 0 0 0 0 0

Note) Skin safety standard (Criteria for international contact dermatitis study)

Criteria Evaluation standard sign Average score Negative Non-irritating - 0 Slight reaction and erythema Smile stimulation ± 0 to 0.9 Erythema + curing Light stimulation + 1.0-2.9 Erythema + hardening + small blisters Heavy stimulation ++ 3.0-4.9 Erythema + curing + blister Strong stimulation +++ 5.0 or more

As shown in Table 6, in the cosmetic composition of the formulation example according to the present invention, the skin irritation degree is all zero, and the cosmetic composition comprising the vitamin K-based compound according to the present invention as an active ingredient is a very safe cosmetic without irritation to the skin. It can be seen that the composition.

In addition, to determine the stability over time for the cosmetic composition according to the present invention, the following experiment was performed. The cosmetic composition of the above formulation example was stored at 0 ° C., 25 ° C., 40 ° C., 50 ° C., in a thermostat of a cycle, and thermal stability was evaluated based on the following evaluation criteria. As a result, the formulation example cosmetic composition according to the present invention was observed only a phenomenon such as phase separation after one month in the formulation examples of the emulsion formulation at 50 ℃ storage conditions was excellent overall thermal stability under other severe conditions. Therefore, it can be seen that the cosmetic composition comprising the vitamin K-based compound according to the present invention as an active ingredient is a stable formulation having excellent long-term storage stability.

Antibacterial and antifungal effects of the vitamin K-based compounds according to the present invention can be used instead of conventional preservatives or preservatives used in the preparation of cosmetics, in particular, microorganisms such as acne bacteria, athlete's foot and dandruff causing skin problems It can be applied to a cosmetic composition for improving skin diseases caused by microorganisms having excellent activity in inhibiting proliferation. In addition, there is an advantage that can be applied to the product as a manufacturing method commonly used in the manufacture of general cosmetics can be said that the industrial applicability is very large.

Claims (6)

A cosmetic composition having a microbial growth inhibitory activity comprising as an active ingredient vitamin K1 (phylloquinone) or vitamin K3 (menadione). The cosmetic composition according to claim 1, wherein the vitamin K1 or vitamin K3 is contained in an amount of 0.001 to 5.000 wt% based on the total weight of the cosmetic composition. The method of claim 1, wherein the vitamin K1 or vitamin K3 is used as a substitute for preservatives or preservatives commonly used in the preparation of existing cosmetic compositions, or for the purpose of preventing primary or secondary microbial contamination of the cosmetic composition. Cosmetic composition to be. A cosmetic composition comprising vitamin K1 (phylloquinone) or vitamin K3 (menadione) as an active ingredient for the purpose of improving skin diseases of acne, athlete's foot or dandruff. The cosmetic composition according to claim 4, wherein the vitamin K1 or vitamin K3 is contained in an amount of 0.001 to 5.000 wt% based on the total weight of the cosmetic composition. The formulation according to any one of claims 1 to 4, wherein the formulation of the cosmetic composition is a flexible cosmetic (skin toner), a convergent cosmetic, a nutrient cosmetic (lotion), a nourishing cream, a massage cream, an essence, a gel, a pack, a powder, Cosmetic composition, characterized in that it is an external skin ointment, patch, suspension, emulsion, spray, hair shampoo, hair treatment, hair tonic, body cleanser, shower gel, or cosmetic liquid.