KR20220038439A - 자연 살해 세포의 향상된 증폭 및 세포 독성을 위한 방법 및 조성물 - Google Patents

자연 살해 세포의 향상된 증폭 및 세포 독성을 위한 방법 및 조성물 Download PDF

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KR20220038439A
KR20220038439A KR1020227005973A KR20227005973A KR20220038439A KR 20220038439 A KR20220038439 A KR 20220038439A KR 1020227005973 A KR1020227005973 A KR 1020227005973A KR 20227005973 A KR20227005973 A KR 20227005973A KR 20220038439 A KR20220038439 A KR 20220038439A
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cells
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ser
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ala
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제임스 바나비 트레이거
알렉산드라 레이다 리아나 라제틱
아이반 찬
앤몰 보라
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엔카르타, 인크.
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    • C12N2502/99—Coculture with; Conditioned medium produced by genetically modified cells

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KR1020227005973A 2019-07-31 2020-07-29 자연 살해 세포의 향상된 증폭 및 세포 독성을 위한 방법 및 조성물 Pending KR20220038439A (ko)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201962881311P 2019-07-31 2019-07-31
US62/881,311 2019-07-31
US201962932342P 2019-11-07 2019-11-07
US62/932,342 2019-11-07
PCT/US2020/044033 WO2021021907A1 (en) 2019-07-31 2020-07-29 Methods and compositions for enhanced expansion and cytotoxicity of natural killer cells

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KR20220038439A true KR20220038439A (ko) 2022-03-28

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KR1020227005973A Pending KR20220038439A (ko) 2019-07-31 2020-07-29 자연 살해 세포의 향상된 증폭 및 세포 독성을 위한 방법 및 조성물

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US (1) US20220411754A1 (enExample)
EP (1) EP4003379A4 (enExample)
JP (1) JP7724765B2 (enExample)
KR (1) KR20220038439A (enExample)
CN (1) CN114450015A (enExample)
AU (1) AU2020321354A1 (enExample)
CA (1) CA3144724A1 (enExample)
IL (1) IL289902A (enExample)
MX (1) MX2022001255A (enExample)
WO (1) WO2021021907A1 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025150751A1 (ko) * 2024-01-11 2025-07-17 사회복지법인 삼성생명공익재단 Nk 세포의 활성화 및 증폭용 배양보조세포 및 이의 용도

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK3143134T3 (da) 2014-05-15 2021-01-04 Nat Univ Singapore Modificerede, naturlige dræberceller og anvendelser deraf
US11629340B2 (en) 2017-03-03 2023-04-18 Obsidian Therapeutics, Inc. DHFR tunable protein regulation
BR112019019917A2 (pt) 2017-03-27 2020-04-22 Nat Univ Singapore receptores quiméricos nkg2d truncados e seus usos em imunoterapia de células exterminadoras naturais
EP3749685A4 (en) 2018-02-09 2021-12-22 National University of Singapore ACTIVATOR CHEMERIC RECEPTORS AND THEIR USES IN NATURAL KILLING CELL IMMUNOTHERAPY
JP7334985B2 (ja) 2018-04-02 2023-08-29 ナショナル ユニヴァーシティー オブ シンガポール 免疫細胞で発現される膜結合抗サイトカイン非シグナル伝達バインダーによるヒトサイトカインの中和
EP3844186A4 (en) 2018-08-29 2022-08-17 National University of Singapore METHOD OF SPECIFIC STIMULATION OF SURVIVAL AND EXPANSION OF GENETICALLY MODIFIED IMMUNE CELLS
CA3120563A1 (en) 2018-11-26 2020-06-04 Nkarta, Inc. Methods for the simultaneous expansion of multiple immune cell types, related compositions and uses of same in cancer immunotherapy
EP3773918A4 (en) 2019-03-05 2022-01-05 Nkarta, Inc. CD19 DIRECTED CHIMERIC ANTIGEN RECEPTORS AND THEIR USES IN IMMUNOTHERAPY
AU2020288829A1 (en) * 2019-06-04 2021-12-02 Nkarta, Inc. Combinations of engineered natural killer cells and engineered T cells for immunotherapy
US12551530B2 (en) 2020-08-14 2026-02-17 Kite Pharma, Inc. Immune cell function
IL300903A (en) * 2020-09-02 2023-04-01 Nkarta Inc Expansion and enhanced cytotoxicity of natural killer cells and their uses
US20240316102A1 (en) 2021-07-01 2024-09-26 Indapta Therapeutics, Inc. Engineered natural killer (nk) cells and related methods
AU2022319878A1 (en) * 2021-07-28 2024-01-18 Nkarta, Inc. Selection of optimal cell donors and methods and compositions for enhanced expansion and cytotoxicity of donor cells
CN116236461B (zh) * 2021-12-08 2026-03-24 深圳先进技术研究院 一种氧化还原型纳米颗粒和活细胞载体及其应用
JP2025525439A (ja) 2022-06-30 2025-08-05 インダプタ セラピューティクス インコーポレイテッド 操作されたナチュラルキラー（ｎｋ）細胞と抗体療法との組み合わせおよび関連する方法
EP4649139A1 (en) * 2023-02-01 2025-11-19 Nkarta, Inc. Methods for expanding immune cells and related compositions and uses thereof

Family Cites Families (7)

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Publication number Priority date Publication date Assignee Title
CN101684456A (zh) * 2008-09-28 2010-03-31 江门罗森生物制药有限公司 一种体外培养条件下扩增人nk细胞的方法
DK3143134T3 (da) * 2014-05-15 2021-01-04 Nat Univ Singapore Modificerede, naturlige dræberceller og anvendelser deraf
HK1245080A1 (zh) * 2014-10-27 2018-08-24 University Of Central Florida Research Foundation, Inc. 自然杀伤细胞的方法和组合物
US20190134095A1 (en) * 2016-05-02 2019-05-09 Cerus Corporation Compositions and methods for improved nk cell therapies
CN106085958A (zh) * 2016-08-04 2016-11-09 英普乐孚生物技术(上海)有限公司 一种nk细胞的制备方法
US10300089B2 (en) * 2016-11-08 2019-05-28 University Of Central Florida Research Foundation, Inc. Methods for high scale therapeutic production of memory NK cells
CA3056591A1 (en) * 2017-03-27 2018-10-04 National University Of Singapore Stimulatory cell lines for ex vivo expansion and activation of natural killer cells

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025150751A1 (ko) * 2024-01-11 2025-07-17 사회복지법인 삼성생명공익재단 Nk 세포의 활성화 및 증폭용 배양보조세포 및 이의 용도

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CA3144724A1 (en) 2021-02-04
JP2022542399A (ja) 2022-10-03
US20220411754A1 (en) 2022-12-29
IL289902A (en) 2022-03-01
EP4003379A1 (en) 2022-06-01
JP7724765B2 (ja) 2025-08-18
WO2021021907A1 (en) 2021-02-04
EP4003379A4 (en) 2023-08-30
AU2020321354A1 (en) 2022-03-03
CN114450015A (zh) 2022-05-06
MX2022001255A (es) 2022-05-10

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