KR20220004645A - 최적화된 초저 부피 액체 생검 방법, 시스템 및 장치 - Google Patents

최적화된 초저 부피 액체 생검 방법, 시스템 및 장치 Download PDF

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KR20220004645A
KR20220004645A KR1020217034791A KR20217034791A KR20220004645A KR 20220004645 A KR20220004645 A KR 20220004645A KR 1020217034791 A KR1020217034791 A KR 1020217034791A KR 20217034791 A KR20217034791 A KR 20217034791A KR 20220004645 A KR20220004645 A KR 20220004645A
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biological sample
nucleic acid
cases
cell
cfdna
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덴 붐 더크 반
마티아스 에리치
폴 에스
짐 차우바푼
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주노 다이어그노스틱스, 인크.
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/501—Ligase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/101—Temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/122—Massive parallel sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
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  • Genetics & Genomics (AREA)
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  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
KR1020217034791A 2019-03-27 2020-03-25 최적화된 초저 부피 액체 생검 방법, 시스템 및 장치 Ceased KR20220004645A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962824757P 2019-03-27 2019-03-27
US62/824,757 2019-03-27
PCT/US2020/024638 WO2020198312A1 (en) 2019-03-27 2020-03-25 Optimized ultra-low volume liquid biopsy methods, systems, and devices

Publications (1)

Publication Number Publication Date
KR20220004645A true KR20220004645A (ko) 2022-01-11

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KR1020217034791A Ceased KR20220004645A (ko) 2019-03-27 2020-03-25 최적화된 초저 부피 액체 생검 방법, 시스템 및 장치

Country Status (8)

Country Link
US (1) US20220162591A1 (https=)
EP (1) EP3947672A4 (https=)
JP (2) JP2022525953A (https=)
KR (1) KR20220004645A (https=)
CN (1) CN113906146A (https=)
AU (1) AU2020245532A1 (https=)
CA (1) CA3134941A1 (https=)
WO (1) WO2020198312A1 (https=)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023136502A1 (ko) 2022-01-12 2023-07-20 주식회사 엘지에너지솔루션 전고체 전지용 전극의 제조방법 및 이에 의해 제조된 전극
WO2024258237A1 (ko) * 2023-06-14 2024-12-19 솔바이오 주식회사 국소적 엑소좀 이질성 변화 측정 기반 액체생검

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CN111526793A (zh) 2017-10-27 2020-08-11 朱诺诊断学公司 用于超低体积液体活检的设备、系统和方法
US12462935B2 (en) 2018-03-30 2025-11-04 Nucleix Ltd. Deep learning-based methods, devices, and systems for prenatal testing
US20240018585A1 (en) * 2020-12-15 2024-01-18 Gateway Genomics, Llc Methods, compositions, and devices for the rapid determination of fetal sex
WO2022197659A2 (en) * 2021-03-15 2022-09-22 Board Of Regents, The University Of Texas System Fluorescence detection of circulating cell free dna including within extracellualr vesicles in biospecimens and liquid biopsies
CN113549674B (zh) * 2021-04-22 2023-11-03 福建和瑞基因科技有限公司 一种检测样本中目标序列整合和突变的方法及其引物的设计方法和试剂盒
US20240301397A1 (en) * 2021-07-23 2024-09-12 The Johns Hopkins University Systems and methods for capture of circulating free dna
EP4373972A4 (en) * 2021-07-23 2025-06-18 Georgetown University USE OF METHYLATED CIRCULATING FREE DNA TO DETECT TISSUE INJURY
US20260078364A1 (en) * 2022-09-26 2026-03-19 The Johns Hopkins University Devices and systems for dna capture
US12435357B2 (en) 2022-12-22 2025-10-07 Kanso Diagnostics Ltd. Diagnostic device for detecting a target nucleic acid molecule in a biological sample
GB2635305A (en) * 2023-07-21 2025-05-14 Tailor Bio Ltd Genomic sequencing analysis

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JPS5977356A (ja) 1982-06-30 1984-05-02 Fuji Photo Film Co Ltd 螢光アツセイ用多層分析要素およびそれを用いる螢光アツセイ法
AU3601599A (en) 1998-03-25 1999-10-18 Ulf Landegren Rolling circle replication of padlock probes
EP1020534B2 (en) 1998-11-09 2011-01-05 Eiken Kagaku Kabushiki Kaisha Process for synthesizing nucleic acid
US6749575B2 (en) * 2001-08-20 2004-06-15 Alza Corporation Method for transdermal nucleic acid sampling
EP2057289B1 (en) * 2006-08-09 2016-08-10 Univ Singapore PROCESS FOR MOLECULAR BIOLOGICAL APPLICATIONS
US20110086359A1 (en) 2008-06-10 2011-04-14 Rapid Pathogen Screening, Inc. Lateral flow assays
WO2013052907A2 (en) * 2011-10-06 2013-04-11 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
EP2684954A1 (en) * 2012-07-10 2014-01-15 Lexogen GmbH 5´ protection dependent amplification
US10993418B2 (en) * 2012-08-13 2021-05-04 Life Genetics Lab, Llc Method for measuring tumor burden in patient derived xenograft (PDX) mice
US9423399B2 (en) 2012-09-28 2016-08-23 Takara Bio Usa, Inc. Lateral flow assays for tagged analytes
US10262755B2 (en) * 2014-04-21 2019-04-16 Natera, Inc. Detecting cancer mutations and aneuploidy in chromosomal segments
US9499870B2 (en) * 2013-09-27 2016-11-22 Natera, Inc. Cell free DNA diagnostic testing standards
CN106132200B (zh) * 2014-03-18 2020-10-30 凯杰有限公司 胞外核酸的固定和分离
WO2017017314A1 (en) 2015-07-24 2017-02-02 Ahlstrom Corporation Blood separation media and lateral flow devices for blood samples
EP3757229A1 (en) * 2015-08-12 2020-12-30 Circulogene Theranostics, LLC Method of preparing cell free nucleic acid molecules by in situ amplification
GB201515557D0 (en) * 2015-09-02 2015-10-14 14M Genomics Ltd Method of sequencing
JP6913089B2 (ja) * 2015-11-11 2021-08-04 レゾリューション バイオサイエンス， インコーポレイテッド Ｄｎａライブラリーの高効率構築
WO2017176630A1 (en) * 2016-04-07 2017-10-12 The Board Of Trustees Of The Leland Stanford Junior University Noninvasive diagnostics by sequencing 5-hydroxymethylated cell-free dna
US20180346963A1 (en) * 2017-06-01 2018-12-06 Counsyl, Inc. Preparation of Concatenated Polynucleotides

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023136502A1 (ko) 2022-01-12 2023-07-20 주식회사 엘지에너지솔루션 전고체 전지용 전극의 제조방법 및 이에 의해 제조된 전극
WO2024258237A1 (ko) * 2023-06-14 2024-12-19 솔바이오 주식회사 국소적 엑소좀 이질성 변화 측정 기반 액체생검

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Publication number Publication date
US20220162591A1 (en) 2022-05-26
AU2020245532A1 (en) 2021-11-11
JP2022525953A (ja) 2022-05-20
EP3947672A1 (en) 2022-02-09
CA3134941A1 (en) 2020-10-01
CN113906146A (zh) 2022-01-07
JP2025093956A (ja) 2025-06-24
WO2020198312A1 (en) 2020-10-01
EP3947672A4 (en) 2023-01-11

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