CN113906146A - 优化的超低体积液体活检方法、系统和设备 - Google Patents

优化的超低体积液体活检方法、系统和设备 Download PDF

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CN113906146A
CN113906146A CN202080039518.XA CN202080039518A CN113906146A CN 113906146 A CN113906146 A CN 113906146A CN 202080039518 A CN202080039518 A CN 202080039518A CN 113906146 A CN113906146 A CN 113906146A
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biological sample
cases
cell
nucleic acid
cfdna
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迪尔克·万登博姆
马蒂亚斯·埃里克
保罗·厄特
吉姆·肖瓦普恩
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Juno Diagnostics Inc
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Juno Diagnostics Inc
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/501—Ligase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/101—Temperature
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/122—Massive parallel sequencing
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis

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  • Chemical & Material Sciences (AREA)
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  • Health & Medical Sciences (AREA)
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  • Proteomics, Peptides & Aminoacids (AREA)
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  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
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  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN202080039518.XA 2019-03-27 2020-03-25 优化的超低体积液体活检方法、系统和设备 Pending CN113906146A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962824757P 2019-03-27 2019-03-27
US62/824,757 2019-03-27
PCT/US2020/024638 WO2020198312A1 (en) 2019-03-27 2020-03-25 Optimized ultra-low volume liquid biopsy methods, systems, and devices

Publications (1)

Publication Number Publication Date
CN113906146A true CN113906146A (zh) 2022-01-07

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CN202080039518.XA Pending CN113906146A (zh) 2019-03-27 2020-03-25 优化的超低体积液体活检方法、系统和设备

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Country Link
US (1) US20220162591A1 (https=)
EP (1) EP3947672A4 (https=)
JP (2) JP2022525953A (https=)
KR (1) KR20220004645A (https=)
CN (1) CN113906146A (https=)
AU (1) AU2020245532A1 (https=)
CA (1) CA3134941A1 (https=)
WO (1) WO2020198312A1 (https=)

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CN111526793A (zh) 2017-10-27 2020-08-11 朱诺诊断学公司 用于超低体积液体活检的设备、系统和方法
US12462935B2 (en) 2018-03-30 2025-11-04 Nucleix Ltd. Deep learning-based methods, devices, and systems for prenatal testing
US20240018585A1 (en) * 2020-12-15 2024-01-18 Gateway Genomics, Llc Methods, compositions, and devices for the rapid determination of fetal sex
WO2022197659A2 (en) * 2021-03-15 2022-09-22 Board Of Regents, The University Of Texas System Fluorescence detection of circulating cell free dna including within extracellualr vesicles in biospecimens and liquid biopsies
CN113549674B (zh) * 2021-04-22 2023-11-03 福建和瑞基因科技有限公司 一种检测样本中目标序列整合和突变的方法及其引物的设计方法和试剂盒
US20240301397A1 (en) * 2021-07-23 2024-09-12 The Johns Hopkins University Systems and methods for capture of circulating free dna
EP4373972A4 (en) * 2021-07-23 2025-06-18 Georgetown University USE OF METHYLATED CIRCULATING FREE DNA TO DETECT TISSUE INJURY
KR20230108922A (ko) 2022-01-12 2023-07-19 주식회사 엘지에너지솔루션 전고체 전지용 전극의 제조방법 및 이에 의해 제조된 전극
US20260078364A1 (en) * 2022-09-26 2026-03-19 The Johns Hopkins University Devices and systems for dna capture
US12435357B2 (en) 2022-12-22 2025-10-07 Kanso Diagnostics Ltd. Diagnostic device for detecting a target nucleic acid molecule in a biological sample
WO2024258237A1 (ko) * 2023-06-14 2024-12-19 솔바이오 주식회사 국소적 엑소좀 이질성 변화 측정 기반 액체생검
GB2635305A (en) * 2023-07-21 2025-05-14 Tailor Bio Ltd Genomic sequencing analysis

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US20150147815A1 (en) * 2013-09-27 2015-05-28 Natera, Inc. Cell free dna diagnostic testing standards
US20180030528A1 (en) * 2011-10-06 2018-02-01 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
CN108138209A (zh) * 2015-08-12 2018-06-08 环基因治疗诊断有限责任公司 通过原位扩增制备细胞游离核酸分子的方法
CN108431233A (zh) * 2015-11-11 2018-08-21 分析生物科学有限公司 Dna文库的高效率构建
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US20180030528A1 (en) * 2011-10-06 2018-02-01 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US20180288982A1 (en) * 2012-08-13 2018-10-11 InnoGenomics Technologies, LLC Method for measuring tumor burden in patient derived xenograft (pdx) mice
US20150147815A1 (en) * 2013-09-27 2015-05-28 Natera, Inc. Cell free dna diagnostic testing standards
CN108138209A (zh) * 2015-08-12 2018-06-08 环基因治疗诊断有限责任公司 通过原位扩增制备细胞游离核酸分子的方法
CN108431233A (zh) * 2015-11-11 2018-08-21 分析生物科学有限公司 Dna文库的高效率构建

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US20220162591A1 (en) 2022-05-26
KR20220004645A (ko) 2022-01-11
AU2020245532A1 (en) 2021-11-11
JP2022525953A (ja) 2022-05-20
EP3947672A1 (en) 2022-02-09
CA3134941A1 (en) 2020-10-01
JP2025093956A (ja) 2025-06-24
WO2020198312A1 (en) 2020-10-01
EP3947672A4 (en) 2023-01-11

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Application publication date: 20220107