KR20210157183A - Novel bacteriophage VPT02 infecting vibrio and use thereof - Google Patents

Abstract

The present invention relates to a novel bacteriophage and a use thereof. Specifically, the present invention relates to an isolated bacteriophage VPT02 with accession number KCTC18823P having a specific killing ability for Vibrio; a composition for preventing or treating diseases caused by Vibrio bacteria comprising the bacteriophage as an active ingredient; a composition for inhibiting or killing Vibrio bacteria comprising the bacteriophage as an active ingredient; an antibacterial agent comprising the bacteriophage as an active ingredient; a feed additive for fish and shellfish or shrimp farming comprising the bacteriophage as an active ingredient; a disinfectant or cleaning agent comprising the bacteriophage as an active ingredient; a method for preventing or treating diseases caused by Vibrio, comprising administering the bacteriophage to an individual other than a human; and a method of lysing enteritis Vibrio parahaemolyticus, Vibrio harveyi, or Vibrio alginolyticus comprising the step of treating the bacteriophage. The novel bacteriophage of the present invention has a specific killing ability for enteritis Vibrio, Vibrio harvey, or Vibrio alginolyticus, and has excellent acid resistance and heat resistance. The bacteriophage can be used not only for the prevention or treatment of Vibrio-induced diseases caused by the Vibrio bacteria, but also can be widely used for antibacterial agents, disinfectants, or cleaning agents.

Description

Novel bacteriophage VPT02 infecting Vibrio and use thereof {Novel bacteriophage VPT02 infecting vibrio and use thereof}

The present invention relates to a novel bacteriophage and its use, and specifically, the isolated bacteriophage VPT02 having an accession number KCTC18823P having a specific killing ability for Vibrio; A composition for preventing or treating diseases caused by Vibrio bacteria comprising the bacteriophage as an active ingredient; A composition for inhibiting or killing Vibrio bacteria comprising the bacteriophage as an active ingredient; An antibacterial agent comprising the bacteriophage as an active ingredient; A feed additive for fish and shellfish or shrimp culture comprising the bacteriophage as an active ingredient; Disinfectant or cleaning agent comprising the bacteriophage as an active ingredient; A method for preventing or treating diseases caused by Vibrio, comprising administering the bacteriophage and the composition to an individual other than humans; And it relates to a method for lysing enteritis Vibrio parahaemolyticus, Vibrio harveyi, or Vibrio alginolyticus comprising the step of treating the bacteriophage.

Bacteria belonging to the Vibrio family are Gram-negative bacilli, mainly when people with underlying diseases with reduced immune status, such as liver disease, alcoholism and diabetes, eat raw or incompletely cooked fish and shellfish contaminated with this bacteria. It is known to cause infection when wounds are exposed to contaminated seawater. In many Asian countries, including Korea and Japan, the incidence continues every year due to the diet of reproductive fish and shellfish, and it is known as a problematic disease due to its high group incidence. Recently, as the reproduction of fish and shellfish is increasing in many Western countries, the occurrence of diseases caused by bacterial infection, mainly in the United States and Europe, is spreading all over the world. This infectious disease is known to be closely related to oysters in particular among fish and shellfish. Since this bacterium actively reproduces when the water temperature rises, sales and distribution of oysters are prohibited in Korea during the epidemic period (June to September) of this bacterium every summer. In particular, due to the recent increase in group food service, the possibility of a pandemic of this disease is predicted and caution is required.

On the other hand, bacteriophage is a virus that specifically infects bacteria, and was discovered in 1915 by British bacteriologist Twort in a study on the phenomenon of transparent dissolution of Staphylococcus (Micrococcus) colonies by something. Also, in 1917, French bacteriologist d'Herelle discovered that there was something with the action of dissolving Shigella disentriae in the filtrate of the feces of a patient with this disease, and independently discovered bacteriophages through research on this, and eats bacteria. Named as a bacteriophage from the meaning. Treatment using phage has been continuously studied only in Eastern Europe and the former Soviet Union since the development of antibacterial agents. As a result, it is in the spotlight as an alternative to antibacterial agents. Moreover, bacteriophage has a shorter development time and less development cost compared to antibacterial agents, and is recognized as superior in terms of bacterial selectivity, persistence and side effects.

As an example, there are domestic registration patent 10-2071706, which discloses a bacteriophage therapy for controlling Chinese cabbage soft disease, a novel bacteriophage that inhibits the proliferation of Salmonella typhimurium, and domestic registration patent 10-2093238, which discloses the use thereof.

Under this background, the present inventors made intensive research efforts to develop a method for controlling Vibrio bacteria that cause enteritis, and as a result, enteritis vibrio (Vibrio parahaemolyticus), Vibrio harveyi, or Vibrio alginolyticus (Vibrio alginolyticus) ), found bacteriophage VPT02 that has specific lytic activity and resistance to various environmental factors (temperature and pH), and when fed to aquatic products such as shrimp, By making it possible to increase the survival rate, the present invention was completed.

One object of the present invention is to provide an isolated bacteriophage VPT02 with accession number KCTC18823P having a specific killing ability to bacteria Vibrio.

Another object of the present invention is to provide a composition for preventing or treating diseases caused by Vibrio, which includes the bacteriophage as an active ingredient.

Another object of the present invention is to provide a composition for inhibiting or killing Vibrio bacteria comprising the bacteriophage as an active ingredient.

Another object of the present invention is to provide an antibacterial agent comprising the bacteriophage as an active ingredient.

Another object of the present invention is to provide a feed additive for fish and shellfish or shrimp aquaculture comprising the bacteriophage as an active ingredient.

Another object of the present invention is to provide a disinfectant or cleaning agent comprising the bacteriophage as an active ingredient.

Another object of the present invention is to provide a method for preventing or treating diseases caused by bacteria Vibrio, comprising administering the bacteriophage or the composition to an individual other than humans.

Another object of the present invention is to provide a method for lysing enteritis Vibrio, Vibrio harvey, or Vibrio alginolyticus comprising the step of treating the bacteriophage.

This will be described in detail as follows. Meanwhile, each description and embodiment disclosed in the present invention may be applied to each other description and embodiment. That is, all combinations of the various elements disclosed herein fall within the scope of the present invention. In addition, it cannot be considered that the scope of the present invention is limited by the specific descriptions described below.

One aspect of the present invention for achieving the above object provides an isolated bacteriophage VPT02 with accession number KCTC18823P having a specific killing ability to bacteria Vibrio.

As used herein, the term “Vibrio species” means that Vibrio bacteria are Gram-negative bacteria. They are short rod-shaped bacteria that are slightly curved or comma (,) in the form of most flagella. (flagella) is known to have motility. Bacteria belonging to Vibrio include water-borne pathogens including Vibrio cholerae, Vibrio vulnificus, and Vibrio parahaemolyticus, and in addition, Vibrio fischeri or It is known that symbiotic/parasitic bacteria such as Bdellovibrio exist.

As used herein, the term “killing ability” refers to the ability to kill or make a living organism disappear. In the present invention, it may mean the ability to kill a strain of bacteriophage.

As used herein, the term "bacteriophage" is a virus that infects a specific target bacterium. The bacteriophage's head, which is encased in a protein capsid, consists of a nucleic acid genome, and some bacteriophages have a tail composed of lipids or proteins. Most bacteriophages contain double-stranded DNA as genetic material.

More than 95% of known bacteriophages belong to the order Caudovirales (Maniloff J and Ackermann H-W. 1998. Arch. Virology. 143: 2051-2063.). The three main families of bacteriophages are distinguished by morphological features, and in particular, by the shape of the tail. Phage belonging to the family Myoviridae has a double-layered contractile tail, the phage belonging to the family Siphoviridae has a long elastic tail, and the phage belonging to the family Podoviridae has a short, blunt tail. (Deveau H, 2006, Appl. Environ. Microbiol. 72: 4338-4346.).

Bacteriophages can be found in water, soil, and all environments inside or outside plants. Bacteriophages attach to target bacteria and inject their own DNA into the host, and self-replicate in the host, causing the death of the bacteria. Bacteriophages are relatively safe against animal or plant cells (Kutter E and Sulakvelidze A. 2004. CRC Press.) and have no effect on other beneficial microorganisms. In addition, bacteriophages are relatively easily isolated from the host bacterial environment.

In the present invention, the bacteriophage may consist of or include the nucleotide sequence of SEQ ID NO: 1 of the sequence listing.

The sequence used in the present invention is construed to include a sequence exhibiting substantial identity to the sequence described in the sequence listing in consideration of mutations having biologically equivalent activity. The term, 'substantial identity' refers to aligning the sequence of the present invention and any other sequences as much as possible, and analyzing the aligned sequence using an algorithm commonly used in the art. means a sequence that exhibits 60% homology, more specifically 70% homology, even more specifically 80% homology, and most specifically 90% homology.

Therefore, a nucleotide sequence having high homology with the sequence represented by SEQ ID NO: 1, for example, a nucleotide sequence having a high homology of 70% or more, specifically 80% or more, more specifically 90% or more, is also It can be construed as being included in the scope of the present invention.

On the other hand, the bacteriophage of the present invention is a novel phage having a different genomic sequence from the existing known phage, by the present inventors Vibrio, specifically, enteritis vibrio (Vibrio parahaemolyticus), Vibrio harveyi (Vibrio harveyi), Or, it was first identified as having a specific killing ability in Vibrio alginolyticus.

In one embodiment of the present invention, in order to obtain a bacteriophage having a lytic power for Vibrio, it is separated and cultured from oysters, and its morphological characteristics are confirmed using TEM for its characterization (FIG. 1), and in 21 strains By confirming the host range through a spotting assay, it was confirmed that there was a lytic activity against enteritis Vibrio, Vibrio havei, or Vibrio alginolyticus (Table 1 and FIG. 2), and the incubation period and release amount in Vibrio enteritis Confirmed (FIG. 3), the adsorption rate was confirmed (FIG. 4), and treated after changing the MOI to Vibrio enteritis to confirm the growth inhibitory effect according to the MOI (FIG. 5).

Another aspect of the present invention provides a composition for preventing or treating diseases caused by Vibrio bacteria comprising the bacteriophage as an active ingredient.

Another aspect of the present invention provides a composition for inhibiting or killing Vibrio bacteria comprising the bacteriophage as an active ingredient.

Specifically, the composition may further include an antibacterial agent, and more specifically, the antibacterial agent is 1-(5-bromothiophene-2-sulfonyl)-1H-pyrazole {1-(5-bromothiophene- 2-sulfonyl)-1H-pyrazole} may be included as an active ingredient, and in the present invention, it may be QStatin {1-(5-bromothiophene-2-sulfonyl)-1H-pyrazole}, but is not limited thereto.

In this case, the definitions of the terms “bacteriophage” and “vibrio bacteria” are the same as described above.

As used herein, the term “inducing” means to be attracted to something and cause something else to happen.

As used herein, the term “disease” refers to a state in which the whole or part of the mind and body cannot function normally due to primary or continuous impairment, and can be divided into infectious diseases and non-infectious diseases. It is known that disease-causing pathogens such as bacteria, fungi, and parasites spread and invade animals or humans to cause disease.

As used herein, the term “prevention” refers to any action that suppresses or delays the symptoms of a disease caused by Vibrio by administering the composition containing the bacteriophage according to the present invention.

As used herein, the term “treatment” refers to any action in which the symptoms of a disease caused by Vibrio bacteria are improved or beneficially changed by administering the composition containing the bacteriophage according to the present invention.

As used herein, the term “proliferation” refers to the proliferation of homogeneous cells by division of cells, and in the present invention, refers to the increase in the number of Vibrio bacteria.

As used herein, the term “inhibition” refers to a phenomenon in which biological activity or activity is reduced.

In addition to the strain included as an active ingredient, the composition may include a known carrier or additive that is acceptable for pharmaceutical, food, or feed. The composition having antibacterial activity against Vibrio bacteria, including the bacteriophage VPT02 (KCTC18823P) in the present invention, has a binder, an emulsifier, a preservative, etc. added to prevent quality deterioration, and amino acids added to the feed to increase the utility Agents, vitamins, enzymes, flavoring agents, non-protein nitrogenous compounds, silicate agents, buffers, extractants, oligosaccharides, and the like. In addition, it may further include a feed mixture, and the like, but is not limited thereto.

In one embodiment of the present invention, the bacteriophage VPT02 was treated after changing the MOI to Vibrio enteritis to confirm the growth inhibitory effect according to the MOI (FIG. 5).

Another aspect of the present invention provides an antibacterial agent comprising the bacteriophage as an active ingredient.

As used herein, the term “antibacterial agent” refers to a substance that kills microorganisms or inhibits growth, and is a generic term for preservatives, fungicides and antibiotics.

The bacteriophage of the present invention not only exhibits high antibacterial activity specifically against Vibrio bacteria, but also has excellent heat resistance and acid resistance. Therefore, the antibacterial agent containing the bacteriophage as an active ingredient is safe because it does not induce drug resistance or resistance compared to the existing antibacterial material, and can be used as a new antibacterial agent with a long life cycle.

Another aspect of the present invention is to provide a feed additive for fish and shellfish or shrimp culture comprising the bacteriophage as an active ingredient.

In this case, the definition of the term “bacteriophage” is as described above.

As used herein, the term “fish and shellfish” refers to a generic term for fish and shellfish.

As used herein, the term “shrimp” refers to a generic term for animals belonging to the suborder Shrimp.

terms in the present invention. “Aquaculture” means the fishery industry in which seedlings are produced or raised for use for food or other purposes.

To the feed additive of the present invention, in addition to the active ingredients, well-known carriers or stabilizers that are acceptable for pharmaceutical, food, or feed may be added, and if necessary, various nutrients such as vitamins, amino acids, minerals, antioxidants and other ingredients. Additives and the like may be added, and the shape may be in a suitable state such as powder, granule, pellet, suspension, or the like. In the case of supplying the feed additive of the present invention, it may be supplied alone or mixed with feed for a unit animal.

In addition, the present invention provides a feed for shrimp farming comprising the above-mentioned feed additive.

The feed of the present invention is not particularly limited, and any feed such as powder feed, solid feed, moist pellet feed, dry pellet feed, EP (Extruder Pellet) feed, raw feed, etc. may be used.

In addition, the bacteriophage VPT02 of the present invention can be prepared separately in the form of a feed additive and mixed with the feed, or directly added during feed production.

In one embodiment of the present invention, the bacteriophage VPT02 was treated after changing the MOI to the brine shrimp infected with Vibrio enteritis to confirm the change in the survival rate of the brine shrimp ( FIG. 6 ), and QStatin {1- in the brine shrimp survival rate experiment (5-bromothiophene-2-sulfonyl)-1H-pyrazole} was co-treated to confirm the synergistic effect (FIG. 7), and the thermal and pH stability of the bacteriophage was confirmed (FIG. 8).

Another aspect of the present invention provides a disinfectant or cleaning agent comprising the bacteriophage as an active ingredient.

As used herein, the term “disinfectant” refers to a drug for the purpose of sterilizing pathogenic bacteria, and the term, “cleaning agent” in the present invention, refers to a drug used for the purpose of cleaning.

The bacteriophage of the present invention not only exhibits high antibacterial activity specifically against Vibrio bacteria, but also has excellent heat resistance and acid resistance. Therefore, the disinfectant or cleaning agent containing the bacteriophage as an active ingredient has superior sterilization activity compared to conventional agents, and can be used as a new disinfectant or cleaning agent with a long product life (life cycling).

Another aspect of the present invention provides a method for preventing or treating diseases caused by bacteria Vibrio, comprising administering the bacteriophage or the composition to an individual other than humans.

In this case, the terms “bacteriophage”, “vibrio”, “disease”, and “prevention” are the same as described above.

As used herein, the term “individual” may mean aquacultureable fish and shellfish or crustaceans other than humans that are or are likely to be induced by a disease caused by Vibrio. However, the present invention is not limited thereto.

Another aspect of the present invention is a method of lysing enteritis Vibrio (Vibrio parahaemolyticus), Vibrio harveyi, or Vibrio alginolyticus comprising the step of treating the bacteriophage. to provide.

In this case, the term “bacteriophage” is the same as described above.

As used herein, the term “Vibrio parahaemolyticus” is a Gram-negative bacillus belonging to the genus Vibrio, which causes acute food poisoning and enteritis in the human body and Vibrio disease in fish. Recently, it has been found to be the causative agent of Acute Hepatopancreatic Necrosis Disease (AHPND), which causes mass death in the shrimp aquaculture industry.

As used herein, the term “Vibrio harveyi” is a Gram-negative bacillus, which exists in seawater and causes many deaths in halibut. In addition, Vibrio harvey is known as the causative agent of systemic diseases, and it is known that it is also growing in domestic farmed fish.

As used herein, the term “Vibrio alginolyticus” is a Gram-negative bacillus, which causes otitis and wound infection.

As used herein, the term "lysis" refers to a phenomenon in which the cell wall or cytoplasmic membrane of the bacteria is destroyed and the bacteria are lysed, and refers to a phenomenon in which the bacteria are killed.

Specifically, the method for lysing the bacteria may include treating the bacteria with an effective amount of an agrochemical composition, antibacterial agent, disinfectant or cleaning agent comprising the bacteriophage of the present invention as an active ingredient, but is not limited thereto.

The novel bacteriophage of the present invention has a specific killing ability for enteritis Vibrio, Vibrio havei, or Vibrio alginolyticus, and has excellent acid resistance and heat resistance, so prevention or treatment of Vibrio-induced diseases caused by the Vibrio bacteria In addition to being able to be used for, it can be widely used as an antibacterial agent, disinfectant, or cleaning agent.

1 is a photograph analyzing the shape of the bacteriophage through TEM.
Figure 2 is a photograph of performing a spotting assay on the ron of the bacterial strain using a bacteriophage.
Figure 3 is a one-step growth curve graph showing the incubation period and release amount of the bacteriophage.
Figure 4 is a graph showing the adsorption rate of the bacteriophage.
5 is a graph showing the effect of inhibiting the growth of enteritis vibrio of bacteriophage through the OD600 value.
6 is a graph showing the change in the survival rate of brine shrimp by bacteriophage.
Figure 7 is a graph showing the change in survival rate of brine shrimp through the combined treatment of the bacteriophage VPT02 and the bacterial communication inhibitor QStatin.
8 is a graph showing the thermal and pH stability of the bacteriophage.

Hereinafter, the present invention will be described in more detail through the following examples. However, these examples are for illustrative purposes only, and the scope of the present invention is not limited to these examples only.

Example 1: Bacteriophage preparation

1) Separation

For the separation of the bacteriophage VPT02, oysters from Tongyeong were put in an LBS liquid medium and homogenized, and the homogenized liquid was filtered using a mixer bag and then cultured with Vibrio enteritis at 30°C with shaking. Thereafter, the culture medium was centrifuged at 13,200 rpm for 1 minute, and the supernatant was filtered using a 0.22 μm filter.

2) Purification and culture

As the filtrate, a medium supplemented with 2% NaCl, 90mM MgCl2, and 20mM CaCl2 was used in LB. In 10 mL of soft agar containing 0.3% agar, 100 μL of Vibrio parahaemolyticus and the sample filtrate were mixed, poured onto an agar plate, and dried for 30 minutes. The dried agar plate was cultured in an incubator at 30°C. To separate a single plaque through the soft agar overlay assay, a single plaque was taken with a pipette, mixed with 1 mL of SM buffer, and then vortexed for 10 minutes. After centrifugation at 13,200 rpm for 1 minute, the supernatant was filtered using a 0.22 μm filter, and the filtrate was infected with the host Vibrio parahaemolyticus ATCC 33844, and then cultured at 30° C. for 2 hours. Then, the host cells were centrifuged at 3,200 rpm for 1 hour, and then filtered using a 0.22 μm filter. The phage filtrate was precipitated by rocking at 4° C. for 12 hours with a solution supplemented with 1M NaCl and PEG6000 (polyethylene glycol 6000), and centrifuged at 3,200 rpm for 1 hour, the precipitated phages were resuspended in SM buffer. . Thereafter, ultracentrifugation was performed for 2 hours at 25,000 rpm at 4° C. using a CsCl solution, and phage particle bands were collected. The collected phage particles were dialyzed with a dialysis buffer and then transferred to a vial and stored at 4°C.

Example 2: Morphological Characterization of VPT02

In order to analyze the characteristics of the bacteriophage VPT02 isolated and cultured in Example 1, the morphology was investigated.

More specifically, a dilution of phage VPT02 diluted to 108 PFU/ml using SM buffer was placed on a grid and fixed, followed by staining with 2% uranyl acetate. After removing the dyeing solution using filter paper, it was additionally washed with distilled water (DW). Then, the morphology of the phage VPT02 was observed using TEM (Transmission electron microscopy).

As a result, as can be seen in FIG. 1 , the morphology of phage VPT02 was observed using transmission electron microscopy (TEM). It was confirmed that VPT02 had a capsid with a diameter of 60 nm and a non-contractible tail with a length of 110.6 nm.

Through this, it was confirmed that VPT02 is an icosahedron, has a non-contractile tail, and belongs to the Siphoviridae family.

Example 3: Confirmation of host strain range and solubility of bacteriophages

In order to confirm the host strain range and solubility of the bacteriophage VPT02, a spotting assay was performed on the lawn of 21 bacterial strains (Spotting assay) and analyzed.

As a result, as can be seen in Table 1, bacteriophage VPT02 did not show lysis activity in other species other than Vibrio species, and among Vibrio species, Vibrio fischeri (Vibrio fischeri), Vibrio vulnificus (Vibrio vulnificus) strain was not infected. The bacteriophage VPT02 showed high solubility against the Vibrio parahaemolyticus strain, but did not infect the Vibrio enteritis FORC 022 strain. In addition, the enteritis Vibrio ATCC 33844 strain showed the highest infectivity among the enteritis Vibrio strains, and it was confirmed that the enteritis Vibrio ATCC 33844 had a transparent plaque type in the spotting assay performed on the loan ( FIG. 2 ).

Through this, it was confirmed that the bacteriophage VPT02 of the present invention can infect enteritis Vibrio, Vibrio havei, and Vibrio alginolyticus, and has lytic ability.

Example 4: Analysis of latency and release amount of bacteriophage VPT02

The incubation period and release amount in Vibrio bacteria were analyzed through the bacteriophage VPT02. Specifically, as for Vibrio, enteritis Vibrio was used.

More specifically, enteritis vibrio ATCC 33844 cultured for one day was inoculated into LBS supplemented with 2% NaCl, 90mM MgCl₂, and 20mM CaCl₂, cultured to OD600 0.6, and then infected with phage VPT02 at MOI 0.1. After incubation at room temperature for 5 minutes, centrifugation was performed for 10 minutes at 6000 x g at 4°C to remove free phage. Thereafter, the supernatant was removed, the precipitate was resuspended, and inoculated into 49mL of LBS supplemented with 2% NaCl, 90mM MgCl₂, and 20mM CaCl₂, and cultured at 30°C. Two sets of 1 mL each were collected every 5 minutes, and one set (control) was centrifuged at 4°C at 13,200 rpm for 1 minute, and the other set was treated with 2% chloroform and vortexed and then centrifuged. After separation, the supernatant was diluted 10-fold (10-fold serial dilution), and 10 μL was dropped into the lawn of Vibrio enteritis ATCC 33844 to perform a spotting assay. Its one-step growth curve shows the dark period (eclipse period), latent period (latent period), and release amount (Burst size) of the bacteriophage VPT02.

As a result, as can be seen in FIG. 3, the dark phase of the bacteriophage VPT02 for enteritis Vibrio ATCC 33844 is 5 minutes, the incubation period is 20 minutes, and the release amount showing lytic power showed 208 PFU/infected cells per infected cell when 50 minutes passed after infection. .

Example 5: Analysis of adsorption rate of bacteriophage VPT02

The adsorption rate of Vibrio bacteria through the bacteriophage VPT02 was analyzed. Specifically, as for Vibrio, enteritis Vibrio was used.

More specifically, enteritis vibrio ATCC 33844 cultured for one day was inoculated into LBS and cultured to an OD600 of 0.6, then 1 mL was taken and centrifuged at 13,200 rpm at 4°C for 1 minute. Thereafter, the supernatant was removed and the precipitate was washed 3 times with 0.8% NaCl, then resuspended in 1mL of LBS supplemented with preheated 2% NaCl, 90mM MgCl₂, and 20mM CaCl₂, and 2% NaCl, 90mM MgCl₂, 20mM CaCl₂ After inoculation in 9mL of supplemented LBS, it was incubated at 30°C for 5 minutes. After stationary culture, VPT02 was infected at an MOI of 0.1, and 1 ml was collected every 3 minutes and centrifuged at 13,200 rpm at 4°C. Thereafter, the supernatant was filtered through a 0.22 μm filter to obtain free phage. In addition, the filtrate was serially diluted 10 times, and 10 μL of each of the filtrates was dropped into the host cells, followed by a spotting assay.

As a result, as can be seen in Figure 4, the bacteriophage VPT02 was adsorbed to Vibrio enteritis ATCC 33844 in 3 minutes, and after 6 minutes, 74% of the phage VPT02 was adsorbed to the host.

Example 6: Analysis of Vibrio growth inhibition of bacteriophage VPT02

The growth inhibitory ability of Vibrio bacteria through the bacteriophage VPT02 was analyzed. Specifically, as for Vibrio, enteritis Vibrio was used.

More specifically, Vibrio enteritis cultured for one day was inoculated into LBS and cultured up to OD600 of 0.6, then 1 mL was inoculated into new LBS supplemented with 2% NaCl, 90mM MgCl₂, and 20mM CaCl₂, and cultured. After one hour, the bacteriophage VPT02 was infected with MOI 0 (SM buffer), MOI 0.01, 0.1, and 1, respectively, and OD600 was measured by taking 1 ml every hour (MOI; multiplicity of infection). This was measured for OD up to 9 hours, and was repeated 3 times.

As a result, as can be seen in FIG. 5 , OD600 increased similarly to the growth of the control group until 3 hours after incubation, and the growth inhibitory effect was first shown at MOI 1 after 3 hours. The higher the MOI, the faster the growth inhibitory effect was, and the lower the MOI, the slower the growth inhibitory effect.

Through this, it was confirmed that the higher the MOI, the superior the growth inhibitory effect appears.

Example 7. Changes in brine shrimp survival rate by bacteriophage VPT02

Example 7-1. Brine Shrimp Preparation

In order to hatch brine shrimp eggs, 0.5 g of eggs were hydrated in 18 mL of primary water and cultured at 28° C. for 1 hour. After the eggs were hydrated, 1,320 μL of 5N NaOH and 10 mL of 50% NaOCl were added to remove the film, and incubated at 28° C. for 2 minutes. Then, 14 mL of 1% Na₂S₂O₃ was added to stop the film removal, and it was washed with artificial seawater solution (35g/L) and sterilized filter paper. The washed eggs were transferred to 30 mL of artificial seawater solution (35 g/L) and then incubated at 28° C. while rotating with a rotator, supplying light and injecting filtered air. After the eggs hatch, the hatched brine shrimp larvae are transferred to 30 mL of fresh artificial seawater solution, 1 mL of sterilized Aeromonas hydrophila KCTC 2358 strain (107 CFU/mL) is supplied as food, and incubated at 28° C. while rotating on a rotating machine.

Example 7-2. survival test

The day after feeding, brine shrimp larvae were transferred to artificial seawater LBS medium supplemented with 2% NaCl, 90mM MgCl₂, and 20mM CaCl₂ in a 12-well plate to perform a viability test. 10 larvae per 1 well were transferred, and after infection with Vibrio enteritis FORC023 strain (104 CFU/mL), the bacteriophage VPT02 was infected with MOI 0 (SM buffer), 0.1, 1, and 10. In addition, in order to prevent the death of larvae due to starvation, sterilized Aeromonas hydrophila KCTC 2358 (107 CFU/mL) was diluted in artificial seawater solution to supply 102 CFU/mL, and cultured with shaking at 100rpm, 28℃, every hour The survival rate was confirmed.

As a result, as can be seen in FIG. 6 , the control group not inoculated with Vibrio enteritis showed a high survival rate of almost 100%, and the control group inoculated with Vibrio enteritis only showed the fastest mortality rate. On the other hand, in the experimental group infected with the bacteriophage VPT02 differently according to the MOI, the higher the MOI, the slower the brine shrimp died, and the lower the MOI, the faster the brine shrimp died.

Through this, it was confirmed that the bacteriophage VPT02 not only could inhibit the growth of Vibrio enteritis, but also had an excellent effect on improving the survival rate of brine shrimp infected with Vibrio enteritis.

Example 8. Synergistic effect of bacteriophage VPT02 and bacterial communication inhibitor

Bacteriophage VPT02 and Vibrio communication inhibitor QStatin {1- (5-bromothiophene-2-sulfonyl) -1H-pyrazole)} was treated in combination, and the effect thereof was confirmed.

More specifically, after preparing the brine shrimp viability test by the method of Example 8, infecting Vibrio enteritis FORC023 strain (104 CFU/mL), phage VPT02 (MOI=1) and QStatin (20uM) were combined and treated Alternatively, each was individually treated and then cultured.

As a result, as can be seen in FIG. 7 , the survival rate of shrimp decreased with the passage of infection time, but the phage VPT02 or QStatin-treated group had a higher survival rate than the group treated with only enteritis bacteria. In addition, the survival rate of brine shrimp in the experimental group treated with VPT02 and QStatin in combination was significantly higher than that of the group treated alone, and at 7 hours after infection, it was confirmed that it had the same 100% survival rate as the group not infected with bacteria. did

Through this, it was confirmed that when the bacteriophage VPT02 and the Vibrio communication inhibitor QStatin were treated in combination, a synergistic effect appeared.

Example 9: Thermal and pH stability of bacteriophage VPT02

In order to confirm the safety of the bacteriophage VPT02 for activity changes by temperature and pH, a safety experiment was performed.

More specifically, 107PFU/mL of VPT02 was added to the LBS culture medium supplemented with 2% NaCl, 90mM MgCl₂, and 20mM CaCl₂ to examine the thermal stability, incubated for 1 hour at each temperature, and serially diluted 10 times, enteritis Vibrio A spotting assay was performed on the rods of the fungus ATCC 33844. In addition, 107PFU/ml of VPT02 was added to the LBS culture medium supplemented with 2% NaCl to check the pH stability, and cultured at 30°C for 1 hour at each pH. A spotting assay was performed by dropping 10 μL at a time.

As a result, as can be seen in Figure 8, the bacteriophage VPT02 was stable up to 60 ℃, but was completely inactivated at a temperature condition of 70 ℃ or more. In addition, it was confirmed that it was stable in the range of pH5 to pH9, 100% stable at pH7, and completely inactivated under extreme pH conditions of pH3 or lower and pH11 or higher.

Through this, it was confirmed that the bacteriophage VPT02 of the present invention is stable in the range of 4 ℃ to 60 ℃ temperature, and stable in the range of pH5 ~ pH9.

In other words, by confirming that the novel bacteriophage VPT02 of the present invention has infectivity and killing ability against infectious Vibrio bacteria such as enteritis Vibrio, and excellent heat resistance and acid resistance, the problem of infectious diseases caused by the infectious Vibrio bacteria It was confirmed that it can be usefully used to solve the problem.

From the above description, those skilled in the art to which the present invention pertains will understand that the present invention may be embodied in other specific forms without changing the technical spirit or essential characteristics thereof. In this regard, it should be understood that the embodiments described above are illustrative in all respects and not restrictive. The scope of the present invention should be construed as being included in the scope of the present invention, rather than the above detailed description, all changes or modifications derived from the meaning and scope of the following claims and their equivalents.

Korea Institute of Biotechnology and Biotechnology KCTC18823P 20200527

SEQUENCE LISTING <110> Ewha University - Industry Collaboration Foundation <120> Novel bacteriophage VPT02 infecting vibrio and use thereof <130> KPA200395-KR <160> 1 <170> PatentIn version 3.2 <210> 1 <211> 120547 <212> DNA <213> Bacteriophage <400> 1 gaaacttttt gtgtaccctg ttagaaattt ataggatggc gtttttgcaa ttagtacgcc 60 aaaagttctt taactgagtt aactcatctt gaatccgttg taaatcttca ggctcacata 120 aatgtaaaca gtctgtatag atatgtatac atccagaata gtttatgtta cctgtgtatt 180 ctgtatctat agagatagtg tccgatactt caaatgttat accattgtta gcatcattaa 240 tctcaatttc ggcaatgtaa tcgcctagat ttaatgtatg ttttgtcata gtaaaacctt 300 actaaatagt gaggatacac aaaaagtttc aaattgttaa agagcgtgtt cccttgggaa 360 cgttagtagt atctcaaaag ccgttaggct tgtcaactac ttttttgaaa aacttttcag 420 tctttctata ctcaactcgc tagggttttt gttgccgccc tgtctcgttg gtatgggtat 480 ataatagcga atttagaaaa agagtctaat cgtttaaatc tttataaatc actagattga 540 taggttttgg ctatcagcgt attttgctca taaatataag gaacgggcgc gcgtgtagca 600 tactttttaa gtaaagtcaa ctaactgtat aaatatacag tatcgctaaa acgctctgta 660 tgcgattcta agcgatttta gcccttagcc ttgccctagt attaaaacat tcttttcgca 720 gcaaatagga aaatttcgaa aaattggcat gatttttgac atggcacgac ttttgcatac 780 actagcaggt ttcatgccaa ctttttatca acagacttat tcacattagc aaactctaat 840 aatactggtt atatactgta tattcacaac ttatccacag gaaaacaaaa gttatctacc 900 ctaaatcaca gcttataaat aacagtatgg cgtttctagg ctgtaaaatc cattctaaga 960 ggttatccac aacctagaca tattaaagta aggcttttat atcaaacctc tcagaatcgc 1020 ttacagcgct tataacgcct gggcatcgtt ttgctggtgg ttagaacaaa atgttattag 1080 acaaataggc tagaattgat actatataga cctacccaac aaggagggct taaaaaatgt 1140 atcgtatatt ttttgatgtt atttggtggt gtgtcagtat ggaaactggc gagtgtgtgg 1200 ctacggctgc tagtatgaaa aaactatttg acaaggtagg catagtatgt ttgaattagc 1260 atctagtatc gcattttgcc tagctatggc aatcggattt tacccttatt ggagattgaa 1320 ataatatggc ttttaactgt aacgaaacac caactctatt gagtcttgac tcaattgtgc 1380 gctatgtcaa ggaagaacgt agcagttttg tagaagaccc agagtctacc atctggtata 1440 ttgacagtga caacgagcta gtggttatgg tacatcctgc gttttgtatg atgttctata 1500 actcagatat gggtatcgag ggaatgtgcg acctagattg tgtaacagat ttatctgcta 1560 tgctagataa ctatggactt gatacaagtt ctatggtatg gcgtgaaatt ggtatagacc 1620 aagtagatta ccattttgtg aactaaatca ctttacatca tgcctaggtt ttagtattat 1680 aaagacctag gcaattgatg cctaaacgga taccgccacc gattttggcg aggggtttta 1740 ccatgaaaat tgatttcctt gttttgtctg aaactattga ttcactaggt tggaataagg 1800 cggttgctat tgttcgcaac ggctttgagg ctgatattat cagcgaggtt gaatgtcagc 1860 tagcacttga caaaatgaaa gccatgttcg agcattatat gtggcgttgg tttgatgtcg 1920 agggtacttg ggaacatcac ttttataaca acgcttttga tatgggtaaa ccgttctcat 1980 tagagcgtga tactggtaac ggctattacc tacacggtgt agctaataaa acaaaaaacg 2040 accaatcttg ggagagcgct ggcaagcgtg ctatagaaga gtataccgta atcggggaaa 2100 atcctgattg cattatgaca gctacaaaac attaattacc gcttaaagcg agggcaacaa 2160 aatgcacaaa caatacctag tagacccaaa agtaagcaac gcttaccaac tggacgctga 2220 cacttctatc agcaagcgca tggaaatggc gcaatgcttt ttgtcaaaca aagagtatca 2280 ttttgacttg acaactaata ttagcttgtt tgagtcagtc gaagaaattc ttaaagctaa 2340 agtcttgact tatgccattg cttttgctaa aggcgcaact aatggcggta tgctttttca 2400 gtatgctaga gccgatttgt taacatggcg agctatctac gagggcgcaa cttttgttaa 2460 tggttggtta aaagcctaat atggaatttg acaactacgc taccgcccta ttgtgggcgg 2520 ttagaaatgg caaaccgttt tttactgtac ggatggacta taacaagatt ttggtgattg 2580 aaaaatgagc gttcacgtta tgacattggg acaaaattgg atgtgcaaca ttatgcataa 2640 tggtcacgtt atacctatga ttttgaagca cgtagaaacc aacgaatttg gtcaagtgtg 2700 gtgcgacgaa aacggtacag cgtacactgt agttaacaaa ctttatccgg tggaggctaa 2760 ctggtaatgt taagacctag tgaaatgtta gttgcctttt tgcaacactt gcaagcgtcc 2820 ggttttcgtc cactgtacta taaaaatgaa atagtggagt caatagagca aatagagtat 2880 gataaatttg tggtacgcta tttgcatatg agcgatgagg aaattcgctg cgatggaatt 2940 ttaatcagtg aatttctgga gatagtacgc gactttcgaa tagattaaaa acgttataag 3000 gtcacttttt agtgaccttt ttcataccca gcaaatgaga atagttatca tttcggcgcg 3060 cccgtcgggc aggttggcat ggttcttgca tggtctacag gcgagcttgt gtgtcgatta 3120 gggcttaccc cttgctaggg tattgctata tagctagagt gggcttagcg gcgcttacag 3180 gagctttaaa cggtattcgg aataatctgc tacctgataa gcactgtata aatatacagt 3240 agttttgcgg tggataaggc tgtggataac tctgtataga gcgttttaga gccatgtata 3300 aatatacagt tgtggataac tttttaataa gcaaaaagcg tgccatattt tatacggtac 3360 gccgtctgga tggctaaacg tctggatggc tagaagtcta ggcggctaaa catctggatg 3420 gctagaagtc tataagcaat cactaattta gggcatactt tattagcaat ctctaatcct 3480 ctgcgcccgt gggtgcgaat ccgacaagtg caaaaacgat gattacatgg cagtaggtgc 3540 aaatccaata ataaaagttt gtggtgggac taggtgcaaa ttctataaaa ttccaattga 3600 ctttctgcgc ccgccagcag gtgcggattc gaccccaaat tggcttccga gggtaggtgc 3660 ggattctagg tgcaaattcg agatatatgg agatttctag ggatatatgg agatttaggt 3720 gctattagag gggaattctg gaggaattta aagttttggg gcgtttggta cagatttttg 3780 aaaaaaccaa ttgacaatta cgggggtcgc tccttgaacc ggtctcaatc cgtcttttac 3840 tataaacata aagccttata caataggtgc aaatcctagg ctttagaatt gacatgggga 3900 atctgagcta gacatgtgct tcgcacaaag atatgctcaa tctggtgcag attctagagg 3960 tgcaaaatct atataggtag atataagaaa accctaggct ctcataggaa agactagggt 4020 aattgatata gcagactaga gggatagcgg ggcgttctct attcgtccat ttggggtagt 4080 tttctgtagt taatagaacc ccctcgtatc ttgaagaggt atgctgcaag ggtggctgaa 4140 gaatctctta cgaaccgtct ttgttctcct ctaaccccga actaaacagc cgaatgatct 4200 ttatagattt cttcttccct tgcacgctct aaaattcata agatttatct ctgctagcgt 4260 gagttgttca taactctcta gctatgcagt tattgtatca tggattctca taaatttcaa 4320 gtatatcttt tgtagcttgc ttggctattc taatgtgtca ggaatttcag cgggtgcttg 4380 tgaagcttgc tgaagttcga gcaagagttc ttgctgcgct tcgaagtcta gctctagaaa 4440 tagctcacgt atatattggg tgcgctgaga accctgacct aaggcaaggt ctgttgctct 4500 aggtttttta gcatcacgaa tatttgctcg tgtggcaatt ttatttaggc tctgaataca 4560 gtctagtgca atcttagtat agttgaactg aaaaccttgc ttacgctcaa taacatgctc 4620 tgctgttaaa tcattctcta ggaagtgctc aacagcagga tcaattggct tctctttaac 4680 aactgggggt aaccatgaac gtttctcata gttcttacaa atccagtctt tatcaatacc 4740 gaatgtagct tcaataccag cgtaatctaa taccagcata ttctttaatg gttttaaata 4800 tacatgtgat accgctaacg tccaaggatc attaggtagt gttttacctg catataatgc 4860 taggaatcgt aatacttctt taggacttgc tattgtttgt ctcgtaattt tcattgttgt 4920 ctccttattt ctcacctgag ataaatgtag cttcaccgtt ttcatctata cgatatatag 4980 cacttttaag ggagttggtt aagtctagtg gctctagctt atggtactct tcaattggaa 5040 tccaccaatg ctctccccca ccaatatttt gtacatatgc cccacttgct agggattctg 5100 ttattttat ggcctcatct ttatgctcat cttctgtatt gcagatgaat gttttgccat 5160 tacttactat tactgattgt tccattgttg tctccttaac tgacaatagc ggctaatgag 5220 ccgctaactt tcttaatacg tctgctatct catgctcgat agctatttgg tcttctgtgt 5280 caatggctag ttgtcgtaac catcgtaata ctcttagatg aagtctgagg atagttttgt 5340 taaccatgta atgtcatcct tattagccat accaaccatg ccgtcgtcat acttaacacg 5400 aaccgtattg ccttgcactt ctagaatttc tacattgtag agttggctgc gtaccttaac 5460 aacccattgt tctttagcgt gcaagtcaat attagcttgt gctgtaaaag gcgctaggat 5520 tgatgcgatg gttagccaaa acttagattt catagtataa tctccaataa atcctctagc 5580 ttatcaagag gaatgtcttc tttgcctact agaacatcgt cttcaccgta gacttcgaag 5640 gtgttgtgtt ttaaccagat agtgattttg acataatcac ctctgccatt ataaacctcc 5700 caaccttcgt aatcgttggt ctggtctata gtcttaacta cgtatttact gcgagtgttt 5760 cttagtattc tcacgggctg tctccagtac cttggtaata accgccataa ctgttctctc 5820 ttctaggcta ctgcatccat gctccaatcc ggcgtaaacg ccgtaaacgt ctttctgaca 5880 ttgtttgact aaggctaatg gctctagtct tgttgttcgc tgccttagac agaacagggc 5940 attagatgct tcaaccatac ctgcaaattc tgctagcgat actctcatat taccaatccc 6000 acacacaaga ttcaacacga catttaagaa ctagctcacg gaactcgata agtagctcgt 6060 ggtcttcaaa gtctttctcg tccacaccta gactgtgttc catgtgagag agaaggctat 6120 ctagaataga gtccacacag tagaataatt cgtaacggtt gccttggtag aacttgtcca 6180 tcatagtctt accgaatact ttcttacaga aagcgtcgta accattatat acatattcat 6240 cgtgtcgctg aataaattta ggagcatcta cataagcttg ccagcgttgt atgagcggaa 6300 tctcttgatt agtaagagct gcgatgtaat tacctgccag tcctaacatc tgaccttcta 6360 aagcttgcat ttcagcaaag atagcggtct taggttcttc aatagtttcg tgacaaccat 6420 tagaaccaca ctcagggcag tattcagcag catcaaaaac agcttcgcaa tcgtaacatt 6480 tcattttcat agactatctc cgtttcaatt tatgtatata ttatataaag attttgatgt 6540 ttaagcaaat caagatttat ttagagagtt ctaacaactt tctctctact tctagaatga 6600 gttcgtcgta ctcccacatt ttctttgtga ctctaaaagg ttcttccccc ttcatgcaga 6660 ttctagcagt atactctttg ttagagtcat ctgtaattaa tagggaatga aaaacctttg 6720 ggaagctata tgctacctca catagcatat tctctatttg atacttctta ggccacatac 6780 cagaaaccat tctcgtcttg ctcaatgttg tggtagcctt ctttaacatg ccacaagcct 6840 tccgcacaag tataccagtc accccactgc tcaggtgtgt agtggaagat ttcagagtcc 6900 acttccattt ccgtaccagt ctcttcttcg tactcatctt cgtcgatgta catagaaacg 6960 tgatcacaag ccatttcgtg gatagcttgg tagaactgtg taagttttgc tggagtatca 7020 gtatcgaata ccactaggtc gtgctgttca atgccgcaaa catcaccatc catgcgtaca 7080 acgtaaaggt aatttctttt cataaacaat ctccgtttct caactttatg taactattat 7140 acgttgtttt aactaactaa gcaattagtt taatctttct ttgaaccagc tttcgtataa 7200 ttcatcagac tttcttaaca gctcccagta cttgcgattt tcttcttcac agtggttatc 7260 cactgcctgt ctgttttctt cgtagtaatt gatatgaagc atataggtgt cgattagaac 7320 atcccaatct tcttcaaata ggataatggc ttcattaaca ctgtcacaca tagtccagtc 7380 accaggctca atgctgtagg tgtaaggtat ttcatcatcc tctataccta gtcgagcacg 7440 catttctaag atgtctttat taaccttacg aacaatagac cagtcaccat catctacaaa 7500 agcatgtcgt agagagtgta ccaaacccca aacgctattt aagtcatttt tatatgtctt 7560 agtcaggttg tacagcactt gagaaggaag gtttagtagc ttaataatta cagggttttc 7620 tttttcacaa cgctcaagaa ccgtttctag gtcattgata gacttggttg taaggtcacg 7680 taggttatta cataggtctt tcataaacaa tcttctccgt ttcaatttat gtatattatta 7740 tatgagattt tgatgagtta agcaactaaa gttttaaacc tagtcggatt agtgcgttga 7800 tttccttttc ttcaaaaata agcctatcag cttgacattc tagtattttt gttagctgct 7860 caattcgatg ctcactaacc tgcggtacta ggcggtacgt tggtggtata ttagtaaaac 7920 aaatagaaag ttgtctaggg tttctagctc cgccaatata ctctctgtaa cgattattac 7980 tacgagcgtt agcataactc atatttgcat ctacataaca aacaccaggt atatcatact 8040 tttctagttt tggctcttcc cagtataaat tacaaacaaa cgcatcacga ctaatagggt 8100 ttacagtgtg tggcttgagt gcttctattg atttttccgc atcaaagtct tctacagtgt 8160 atgtttcgta tcttgtattt agtttgaaca gaccccactt aacaggacgt tgtttattaa 8220 tctcaactct gtttttaagg aatttctgtg ccaagccgta tagttcaatg cgatacttac 8280 ttacttcttc atagaagtct ttttccatct tctcaaagag tgcacgacgt tccttgagag 8340 tatttctaag ttctcgtaca gcgcacttga acttaggtgt catagtgtat tctagtttca 8400 ttttataatc ccatctttac cgattattgg tgcttgcctt gcggcattaa tacaaataag 8460 tacattaaga agagcaagca taaaaggagc cccaaacccc attaaaatag ggatacttac 8520 tgctgctacc ttccacacat ctgctggaat atcttttaac atgctactgc tccgtaagtt 8580 aggttaacaa accaagcctg tacaacatcc cataatgttg ggtttggttt agtgattgat 8640 ttagctttac tatgaaattt attgttagct cttagacggt cagcccattc tggattattt 8700 tccgtaggtt tacgagttaa cacatctgct gcgttactaa agatatgtcc cgagtgagct 8760 ttacaatgta ctatatcaat gccgccttct ttaatacggt tgattgtttt agtgtataga 8820 gcaaccttat tggcacgaga ggttttcttt ctagttttct tacagctagg aatagtacca 8880 cgctctagtg actggataag gggctcatgg tctgtatgta ctgtgacatt ctgtaagcct 8940 agctcatcaa taatatctaa acaaatgttg atagcttcta gctcaccttg gcatgagttg 9000 ctttggttag tccattgtgt ttcgtaacga ttataataaa caccacgacc ttcttcaact 9060 acaacaacgc cgataccaaa tggtgcccaa tcacagtaca tatcaaattt cataagacaa 9120 tctccgtttc tcaactttat gtatattatta tataagaatt tagcgtttga agcaattcaa 9180 gatttttgga attaagccat caaacaaggt aaaaagcgta cctaaaataa ccgcagcgaa 9240 tgtgcctggg aatagtatca tcgctgtagg atgcttccac ccatctaggt tgtacttctc 9300 attagatgca tcatataaca taaacagcat ccatacaaaa cataaattaa aatataaact 9360 aatcattatt cttcctcctt ccagtaacca taagatgata ggatttcgaa gccttcatac 9420 tctgcttttt gtgctgcttc aatagtgctg aactcagtaa cacagcacag gtgattagag 9480 tgtgtacaaa cccaccactc gttacccatg tactcgatat agaactcatg gcgtcctgct 9540 gagtattgag aatccttctc aaagttgctg tagaatgtat caaagtcttc agttgcagtc 9600 tgacttagca gcatctgtac gcggaaaatc tgttggatgt ttgacataat cttcttccct 9660 ctctcaactt tatgtaacta ttatacgcga aaagggctgc cgaagcaacc ctttatttaa 9720 gttatttaat ggaaatcggc tctgcaattc ctgtttcgaa agtcgcttta gtaccttggt 9780 caaggacaac acgacattta gtgtcaatat ccgctttagg gaatccagcc attcttacga 9840 acataccaga ccagaagacg ttacgtagtt tttcatactc accgcatacg tcgattttct 9900 tagtttgtga tagtcggaac tcatcacgtc cagcttccat tgctgcttgt aggttagtga 9960 acatagaaga atcgaactgg atgttctgct cttgaatcca ctggaacatt gctttactac 10020 catcttcgcc gtaacgacct tggaaggtag ttttaatttg ttctttcaga gcgtcagagt 10080 acatatccgg tacttgagcc atttctttaa tctttaaggt atagttgcta aggacgttct 10140 gtgaatcctt atcaaacttg ttaatctggg cttcgaagtt gacagccgtg ttatgtgcag 10200 agatgtagga cgctgctggt aggccaataa tagctgctag gaatgtaaca acgattagtg 10260 taacaatttg ccatgttttc atatactata ctcgctctct ttcatagttg tttgctaact 10320 ggtaactaat aataccagta ataaataggt ttacaatgat attaatgaat actaaccaag 10380 ccggtgggtc gatattctca atcatgtact cgaacttttt gtttggtagt ctagtgaatt 10440 tctcatctac caaattaatc tgttctttaa ataactcaat gctgtatggt ttggttattg 10500 cgttatttct cagctgtaca tgcaagtctc ggttatccat accatctgca aagcttgtag 10560 atttaaacca ttgtacagtg tctcctttca tgccaaacat catgagtaca tcgtttttct 10620 taacaccgcc ccaatgagac agtaagacat tgaagaaatc aggattttga ttagtaaatg 10680 ctactatgat atttacttgt tttttggctc ctaattcttt cattcgttgt cttaggtaga 10740 cctcttcaag tctagtgtct agtttggttt ggttaattac tgttttgaca cggtaataat 10800 caaacactct aggatagctt accagaatat cttggtagtt tttgtcagtt tcttgcttag 10860 tgtatagtaa ggagtcctta gcccctttca agtagttaac ataagcatgc gctgcgctag 10920 ctggttcgcc aatttgtact gctgtgaaac gtggtggctc tgtagtacct tgacggtcaa 10980 cacggtctat agtgacatta cctacagttg ttttaacatc ccagtctaca tcataactat 11040 ggtcataaca agtatcatac actgtggtac aagacctgtt tttaccagag cctgaacaaa 11100 ctgttctagg gttgcatgga taactgtgct cacaagatac ttcaacaggt gctttactct 11160 caacataccc atttaatatc tctgtatccc acatccctga atatttggac atttcggtaa 11220 gccctgttag gagcaacgag acaattaatg tagctgctag tcccatgcca atttctttgg 11280 gtgttatctc atgtttgtaa tataacttcc acccaaacac tgagaggaca gaaggtatta 11340 atataagtaa aaagtaaaac attttcaatc tccgtttctc aactttatgt aactattata 11400 cttgaacggg gctgttttag caaccccgtt tttcatttaa ttacatctta gagaagtaaa 11460 attcacgggc tttctgactt agaagattgc caacttcttt catagtcaag ccattggctt 11520 caagcacatc tttttcttcc ttgtagatgt ctttgttaac ccaaccgatg aacttaccag 11580 ttagtttcac atctagacct acttcctgta gaccttgctc tagacggttt tcagtaacag 11640 catactccac gaactcttgg atgctgcgca gtttttcagg gtcaacagat gctagttgtt 11700 ttactttgct aacactgtgt ttctgaccct taaccttgaa ccagctgcct gggttgtctg 11760 ccaatgcgga accgactgga gtccatacaa taccttcacc cacaccgctg acaccgaagt 11820 atttaccaac tggacactct ttctcaactt ctagagtaag ctcttgtagc tgctcagtgg 11880 ctaattgagg catagaggta ttgattttga ttcggtaaca tccgaagtcg taagcattgt 11940 agatacgact ctcatgcagg cttacattac taaacaagtg attaggtagc caaccaatta 12000 gtttaccacc attatcttcg tcattaccaa cacggaagcc aaagatagtg aagaatcggt 12060 ctacttcgct aacagcgacg cctttctgga tgcctttacc tgcccattca ccagcgattt 12120 cgatagggaa ctcaggctgc tcattgtact ctagttcgta cttagcaatg aactgtgcga 12180 ataatttagc tacaggttgc tctagcatgt agttgcagaa tccactgtta tcgttgtgag 12240 tatctagcac acggctttta gactggaaat agatagtacc atcttccaac attaccatac 12300 tgccgttagt accgtggatt ttaacagtac caaccatagg tacgatagaa cctgccggag 12360 tattgtgttg taggaattta gctgcgttac ggaattgacc agtgcttggg tatttaattg 12420 cttccattta tcttctctct tacttagtta cacattgtt attaaacact tctagtgctt 12480 tatcttttgc ttcttgtggg tagccagatt cgggaatctc tgcaagctct tcacaagtat 12540 agtcttgcca gtattctgtg ctgttgatgt tgcctttgat aagctcacca acaaatagtc 12600 ctgcgattag gcacaaaggt agcattaaca ttctcatagg gttactcctg gtagtagttt 12660 gacaacattg tcgtattgtg agccaataaa tttagctgtc tttttatcat atcggtattt 12720 gaaagtatag cagcgacggc tattgtatgc tgcctcaact tggttaacag taaattcagt 12780 acacaagatg ttagatagac tacgagcaac atcatggaac gtatgaaatg tatgacagcc 12840 tttgatgtta ccattatggt actcaatcat acgtgtcatt gatactttaa agccacgctc 12900 atcttcaaag aacttgtgac cacgttcgtt ctgagttagg tagaagccta aaacgttagc 12960 aaaatcaatt aggctcgcca tttgaagttt tagtgtatct ttaatcatgc gtatttctct 13020 tctgtttctt taagattaag cttagctgcc gctagtttgt atcctgtttc gcctcgctga 13080 acttttaatg tataaagctg ctcttctagc tcggctattt tattatctag cgtgttggct 13140 tgacgttggt attgcttttc tacttctctg gctttccata gtagatattc agggaagtta 13200 gacttaacca tgtagtgtac tttagggtca tattcttcta agtagcagta agtggtgcgt 13260 tctactgttt cagaccaatc ttcataccaa ccaccttcgc cgtcacaaga ctcacaccca 13320 tcaccctggc agtccatgca catttcgtac tcttctttac cgtcagcgta agtctctcgt 13380 tcttcccata aataatcttg agcatcttca cgggtcattg gctcgccgtt attcattagg 13440 tgaaagctgg acggagaagg tgtatgccat atacgaccac taccagctcg gtaaacccat 13500 actttctttt ctgtattttc catgacaatc tccgtttctc aactttatgt aactattata 13560 cgtttattta actaaacgag caagtgaaga attaagtttt ttgctaacgt cttgtacgtc 13620 ccagtggtgg tcaaggtcgt ccatttctat aataatttta ccgaaatgag aatcttcaat 13680 ctcccacact ctcttagtag tattgaactt agccacacca aaaccttcaa tgaaagggtc 13740 gcctaggcag tccttttggt ggtagaacat tttagctaga ttctctgcta agtcaccaag 13800 gtcgtcacag ccccagaagt aattacgcca tgcatctttg gcattgtcat caataaagaa 13860 ttgctgtgcc ttctcagggt cacaaaatac tattactggc tctgtttgta ggtttacttc 13920 aaatgctttc atatcttcac cttacatgta tttttcaaat aggtagttct ttagattacg 13980 ttcacaacgg tttagctcgc cttgtaaagc acttttagca agccaagcat cctctagctc 14040 acgcatagct ttatatgctt ctttgcttac ctttaggccg tctgtacctt ctgcttggat 14100 tagatttaag cgactaatct tagccacacc agcagcataa ctatgtaccg ccttaccaag 14160 ctcgtcacgt aagcgttttt caatcaaatc taccattatt tatcttctcc aatgtaagta 14220 caggatgcgt taagtttatc aatatcccct ttagtagttt gacggatttg gtaaactact 14280 tgttctttgg cttgctcaca ctgctccttt gtagggaacg tttcagttgt ggtagctagg 14340 aagccgtact tgaagaacgt ggtagcgaag taagcagtaa cactcattaa atacataaac 14400 aatctccgtt tctcaacttt atgtaactat tatacgttta tttaaggtat ttagcaatta 14460 aattttaatt tttcaaagat ttcaatagct tctataagct ttttattcgt ctcgatcagg 14520 ttgttttctg ctttacgcaa tgtttcttgg cgggcggtgc gctgtagctc taggtctttt 14580 actttcttct ccgccataaa tatggtgcga cgttggatgt atgcttcacg gtctaagatg 14640 aagtctttag gaacaacaat aatgttaccc atttcataat actcaccaca aaatctctgc 14700 atacgtagtg ctagaccacc gtcaggtcgt acttttaaca gtggctgata gttactgtta 14760 tttctattat ccatgatacg aacggtttca cccgtaaaca actcagggtt atcacagatg 14820 aatctacgca tattccaagc atggtagcta ataaacttac gcatctcact ttctagacgt 14880 tcttttttgg ctttaaagtt atctagttga tagatagtaa tatccttacg cttcatttcg 14940 cggataagag ctgcgcgttt ttgtactgct tctacgtatt cttggtaatt cataaacaat 15000 ctccgtttct caactttatg taactattat acgttaaaag gcttgccgaa gcaagcctaa 15060 ttttaattta tttactcgaa atgtgagtca attcgagcac ggatttcagc aaggctagta 15120 cgtttaacaa acttaccgtc cttgaagata gtttttaggc agttctcatc cgaagcttcc 15180 tgctctggag taacatctgc tactagacgg tagctatcgc cgtctttaac aaccatcagc 15240 aagcctttag cagacttttt gctttggtca gttttagggt ctttgctaat agcaacgtgg 15300 ttgccttcac caacttgaat gtcggtagct ttaatagcaa aaccatgagt atcacgagta 15360 acgtattggt atgtgaatga accaatacca agaactacgc taggaacgaa acctttagca 15420 agcaaacgct cgataatttg gtcttggcgt tctagagtaa tagagtcacc gtaaatagct 15480 ccaatgtgtg agtctagaag acgtagaccg tcaacttctg tacccccgaa tgtatcccat 15540 aggcattcga ttagaccttt aacttcgtgc tcaggaattg gtttgccaaa accaacacga 15600 ccatctgccg caattgaagc acgacgccat tgacctgcaa tatctactac cttaccttcc 15660 acttcaaaga agccatcaag tttagcatac gcttcgtcga tagtgtcata gaagtatggg 15720 tgtttttcga taccgcacaa aatttcaact gggtcacctg agtctggacg aataactact 15780 gtaccatcac gagacatgat taagtctttg atttgtggta ggtattcagt tatcaattgc 15840 cagaagtccc atgtatccga aacgatagag ataatgccgt ttggagtaac gttactaatc 15900 aagtggatta gtgatttaag ctcgttctct gtttcgtatg aacacattac tgagtgctca 15960 gtagcatcaa cggaagccat aactagctct ttgtcaatac gtgcatcgta gtactgctcc 16020 attgctaggc cagatacgat agagtctgta cccacgaatg aagtcaagtg accgttctgt 16080 tctggtgcaa acatacccca tagaccacga gctgagaagt catgacccat gaatttaatc 16140 ataccacctg cacgaagtgc tggtacacgt tcgaaacgct cacggtacgc aaatgctgta 16200 gttgcagaag ttactgctgg ccaatactct gcactgaacg gagtttctag caagttagtc 16260 acccaaccta gaccttccac agtgttgtga atagtgtatg gtggtacgcc ataaggtact 16320 agctcgcctt ctggtaatgc tttaacttcg attggcaagt agccaacatc gtgtagttta 16380 gcaatgtgtt ctacgctgat tttcttacca agatagccct tgataacacg cagatattct 16440 gctaggactt cttcttttgg taggttgaag aagttctcat tccatagatt gtttagcttc 16500 tgaagagcca tttggatacc gaagttaact actgctgcgt tgttaggcac gttagagtgc 16560 ttgttaaaac gagctgtacc gttagagtac acgtgagtag tgcctgggcg gtaagcaaat 16620 gggtgaaata ctttgtaacc gtcagtgata agtgctgcta ggtatggtac tttgtaagac 16680 atatattatt tccttctctt taatttatga atatattata cgtaattatt gctgttcttg 16740 caatttaaga ttttctattt ctcgtaaagc gtactcaagg gcatctgtgg cttcccgtag 16800 cccgcactta tcatctagga atacgttata aaataattta ccgtctgttt taacaggaga 16860 cttgttaaca tagggggtgt ctagtccttg atcttgtaag aactctacaa gctcagggtc 16920 atgcttaaat cggcatgtat aacatactag tgtgtgcccc atatgctttg ctcgacgtag 16980 gacattgcga acatgcccta ctaatgccgg attaccaaac gttttgtcga agtctgatac 17040 ggtatcgtca aagtcgaaag caatgataag tgccccgtgg cacttccatt gatgtactaa 17100 tttattacct aaatccatta cttatcctta ccttcgttaa atgctagaac atttgctgca 17160 ttgatgtaac caccaatgaa gttgtgtaca aatacatagt cgatatcttc acgaatcggg 17220 tctagacctt tagagaagat accatgagta acgtataaac ctactttctt agctccacgt 17280 tcacgtagtt tcttagccaa gaacttaaat gatgctccac cgtctgaaat atcatcaacg 17340 attaatactt cttctggtac agttttctct accaaatcac attttacaat gtcacctgta 17400 gttacgtcac gaattttaag cccctgaacc cagaatgtat ggtctagtgc cttggctaca 17460 tcaaagattt tctttgcagc acctaaatct ggcgcacata aagaaaagtt aggcatgtaa 17520 cgacgaatat gtggctccaa catactcact agtgctgctt ggtctaatac tgtagcgtta 17580 ttaattagtg caacgctaac atctgagtgc gggtcttcta ccataacttc atcgaacttc 17640 atgttattaa taaagtctgc gaatactttc ataggtagag gcattcgggt tgcaaattga 17700 cggtcagcac gagcattagg aatatacggc atgttaagac ctaagcgttc ccaatcaata 17760 cattgctcag cacgcatacg ctctagggca tcttttacta acgctagacg tacaatatca 17820 cgagttacat ctttgaaacg aacactaaca gatacgaaag tagatttagt taagtctaca 17880 tttggtttgt taatggtgca tgtttctgct ccatccgaga acgtggtaaa atctactgtc 17940 aagttgtaag atttagtacc gttacacata atagtaatat ttgacatatt gtttccttct 18000 ctttaattta tgtatatatt atacgtaaaa aaggttgccg aagcaaccta agttttaaaa 18060 tttgtattca accatagcag tgaagaatgt accaccaact attaccgtcg gactccagtt 18120 ctcgttataa tcgaatttta tgtaaggcaa cgctcctaga cacattttgc ctgagcaagc 18180 agccagtgtt tgattacttt cgtatccgta aattagactc ggtactacac cgatagtaat 18240 accgtcccta tgtatactaa aatctttgcc tagcaatagg ctatgattct cgaaactgtt 18300 atagaagtaa ccaccaacat acccatcttt ttgatagaat agtaatggat ttatgttatt 18360 acggtcatct tcaaagtgat aagatacacc acctagataa atactatctt cagcagcaga 18420 ctcgaaagag aagcctagcg ttagaactag gcatactaaa gttactacat attttatcat 18480 aatattattaa ttcctgcaac taagaacact ataattatta ccgcgtatcc acatactacg 18540 aagaaagctt gggtatcccc caggtcactc cacgtactgt aaatagggca cttacgtttg 18600 ccaaaaaact ttgtatcacg ttcttcataa tgtgacatac gcatcatacg tataaaacta 18660 cgtactacaa tagttgtcac accagtaatt aggaagaaca tagtccactg tgatatactt 18720 aataccttcc atagtataat tgcagcagta aacttacctg cgggggtttg caaaaactca 18780 ttagtagcta cacctacttc tcgtgctgct acgcctattg ccttagcaaa ctttaatgat 18840 atttcagacc acttatccat cttttcgacg gtagcatctg ttacatctcc tgggatttgt 18900 gtgatggtat caagctttgc ctgctcacac tggattatca tttgttgttt agtggcttta 18960 tctaagccac taatattatt cactgattga gcacagctac ctgcaaatgc gttaaatgat 19020 agcgttgctg ctagtaatac taaaaacttt ttcattagtg atgatcctct gtttctgtgt 19080 tatcaatttt aagcatttgt ttcactgccc aatctagtgg caggatacga ccatgagcat 19140 caaagcccac atccatagag cgtccagtgc cttctagtga cccatgagag tgcccataga 19200 agtggataga cccacggtgt tgttggttcc atacacgcat cgagtagtgg aacatgcaga 19260 ttttgatttt attataagta acttcaagat actcaaaacg atttggtgat ttcacctgat 19320 tacgtacagt tttgtcgtgg ttgcccatta caaatacaat agtaccattc aatcggtcta 19380 ggattgactg tactttctcg atacctgcga atgcaaagtc acctacgtgg aatacaacgt 19440 cgttctcacc aacaacagag ttccagtgtt cgataagtgc ttcatgcatt tcttctacgg 19500 tctgccaagg acgagtattt gggcagaagc gcataatatt attgtggaag aagtgtaagt 19560 cagaagtaat aaagtaatta cgtgcatcat ctaacttcat cccgaattta gctacaaagt 1920 ttttcatgtc ttctccttat aagcgaatgt tagcacgtag tcgttgacgc tgacgtgtca 1980 atgtttcttc tggtacatcg tgtaccgagt cattaccgtg acggttctcc accactagag 197440 tagtcacctg atagccgtgt gtctctgcta agtcaaagta cggcttcatt tctttttctg 1800 tagtgaaagt gttatgtaca ataatgtggt gcagttctag aaccataaga ccttcgactt 19860 ggtcttgaca ccattgatgt gccgtatgca gcttattcac atcaaatagg tatttacctt 19920 caaacatgaa gtagtcatca gcagcgacag cacgagagta tcgcatacct tgttccatgt 19980 tcttagctag agtggtttta cctgagcctg acacaccacg aattaaatat agatttttca 20040 tagacattct cctcaatttc tacagatatt atatcaaagc ttccttgctt tggcaattta 20100 aaaagcgtta tttttatcga accagcactc aatcagctca cgacgttcct gttctttcat 20160 ggtcttccat aggtctcgac cgatgtactg agctacatag tctacaaagc caaagaactc 20220 ttcatccttc aagttcttct tgaattgctc tgggttgtcg aataggttct ctacacgagc 20280 tttacctaga cgcattagca gctttttaga caaatagtgt ggagtcttgt atttaaacaa 20340 gtgctcgtca ttaagtgctg aacacaccat aacaccctcc gtcttagaag ttttagcacg 20400 agccaagatg ttttctagag tatctagacg gtgttctgga cgtttccatc caaatgctag 20460 tgctagctta tctagttctt tctctgtcat tggctcttct gcttcatctt caccttcacc 20520 agtatgacga cagccaatta gatagatacc agcgtgctct ggtacaatat gtgggtcgtc 20580 tggatgacat acttcaaaca ttagagtaaa atctggatgg atttcatcaa tgttacgagt 20640 atgctctaag aacatctctt taaccatttg tgcataggta gagtctagtg taccagtagt 20700 tgaatacagc ataccatact cagacgaacg agtaacacaa cccaagaacc cattaagctt 20760 gtggacttca cggtataagc aatctttgcg taggttgtta ccagtaccat tttcaccgta 20820 gttgaacact ttagtgaatg ggttgacaat aacattccag tcagcatcta ctacacgacc 20880 acgacagtct agaagacgtt catcaaggtg ccataggttc ttgaagaata ccgaacggtc 20940 atacttcaat acttttagtc cattgtcgta ttccttagtc ttaactaagc cacggtcaat 21000 caaatcttgt acgttaaatt tcatactaaa tctccaccac attcattaca ttcaccaact 21060 tcacccatgt cgccccacca catgcagtct tcacagcgaa atagttcgaa tttagctgct 21120 aggctaggga agtcccagtc tagctcatca cgaacttcat cgctgaagcc aaactctttt 21180 tgccagtatt cagcatcaca acctgtatta caagtacctg caactgaatc aacgatttcg 21240 tattgttgaa ctttgtttag gtgtgtatcc caaaaatctg ccataagact atctccgttt 21300 ctcaacttta tgtaactatt atacgctttt taacgttatt tagcaattaa ataattaaat 21360 gttctaggcc gttaggttca aagtgatttc tgataatacg atgatttacc caaccaccgt 21420 tatcccattc cattactgtg agtccgtatt ttctgtctcc gccatcaaag actgagccag 21480 tatcaatcca catttgatta ccaatgctag tgacacagtc acggacacta tgcccatgaa 21540 tagtgtagtc aacaccgcgt atcgtagaaa atctacgacc tttgatagcg tcacgtcccc 21600 aaactgctcg attatcgtca gggaagtatt tgccagctct ttgaacatag tcatcccaga 21660 cttctgttgg gcattctgca tgtgttactc caaatttagt gttttcgtat ctaagtgtaa 21720 gaacaagagg aaattcttgc tccatttttc gcagaatacc ttcaatcatg gcttgtggaa 21780 agtctagaga ccatccacca ccattcataa tccagcttgc tgcctggttg ttttctccgt 21840 gcagtaaccc ttggatagcc atatcttcat gattacctcg aactgctaat gcgttctcgg 21900 taaatagaaa gaagttaact acttctaggt tctgttctcc acggtcgaat aaatccccta 21960 cagagacaat aatatctcta tagttaaatt gtacttggtc taggaagaac ttcagggctt 22020 tccagtcacc gtgcaggtct ccaacaaagt aaatatttcg tctatctgta atatcaatta 22080 cgctcatggt aatattgctc ctataattgc acaaataacc gctactggta agctagtagt 22140 aaaaacatag cctattaatg ctagtatgga cgcccaaatc agtctttctt tcatatttga 22200 ctcctaggtt gcaaatgtgc agatgattta gctactgcct ctactttact ctcaaagaat 22260 ttttcaaaat tatcatgagg atagcaaaat tcgccagatg tgactttaac tagccctatc 22320 atatgcgaga tttcacatcg ccactcacct aagtctgttc tatggagtgt aatatgtaaa 22380 gtaggttcat cttccaaaat attcttatta aaactagccc atgtatagca tccattatga 22440 gaagctatgg agtagccctt ctctgaaagt gattttttgt gtttttcaaa ttcagccttt 22500 attttatcaa gttttactag atctagcaat tgttgttttt cttcaagtga taacacacta 22560 aatttctttg caaattggtc tatattcgcc atactctttc tcctatagta atgccacgca 22620 ttttattaac ataccaagtt ttgaattgta acccttctat tgttacaaca ggtttttcat 22680 acatttggtc tacaagtaca gttttaatat ctttagatat gttattcaat gcaagatcat 22740 cttgtattga ttgtagtatc ctttgttttt ccatctccgc ataaataagc ttagtgttgc 22800 gttctagttt gtctatcatg ccccaaaaag ctaccgctag aagaaatact gtagccataa 22860 atagagcggg tatattagta tagtacagta cgatagacgc tactacagcg aaatacatac 22920 agatacctgt ataacatata cctttgcaaa tcttacccgc gccttgccgc caattcatga 22980 aatcctccaa gtgttacgtg tgcgtatacg caaaaagcca cccgaaggtg gctaattatg 23040 ttattattta gtgacttgtg tcttaaccat taggtctttc gccaccattg cgttaagcat 23100 ttgttggaac tgcgaagcgt caccgtttgt gccctgtgca tcacccattt ggatagttgg 23160 gacagcacgt ttagcgtaag catcagccca tactttttga gtttgaacat aagcttctag 23220 tttttgttgt agagcaccat ctgattgcat catacgtttt ttgtcattag ctcgcgctac 23280 agacatggtt gtaaatgctt ctgcttcttt cttagcagcg atagctcgtt gtgtttgttc 23340 ttgtacaagt tgttttgcaa cttcaacctt acgtttagcg ttggtaatag caacttcttt 23400 ttcacggtca gcagcgattg tggctttttc tttaatctgt agctgttcgt atttaacttt 23460 agcgatagct ttcttacctt tagcttctac ttctttggtt tcccaatcag ctcggtcagc 23520 gttagctttc gcagtaatga ttgccatttc cgcatcgcgt tttttagcaa tctgttcttt 23580 ggtcttatct tcgaagtccc atgatgtaat ctggaaacct gttactttta gaccataatc 23640 ggcgaacata ccatcttggt actcaggagt aataccatct ttttggtatt taatggttgg 23700 caccattttc ttctgaatct tgccgtcttc catttcgact ttcttctctt ttaggacggt 23760 agagaagcga cctttttcaa cctgcattcg tgcccagttt gcatagtcat tacgacgcac 23820 tgcgtaggct tcttctgagg tcattagacc tgctgtcagg ttaagacctt gacgtacttc 23880 acggctgatg attttactaa tcaaacctgc ggggctattt gcagctttgt gaagcgcaat 23940 catattatct ttgtcgttag gtagagcgat cattactgag ccgtctacat aaccgatacc 24000 accgtcttga tactgaacgt tgataccttc tttaattact tgacgacctt gggaatctat 24060 aattttagag tcgggtgttt cgtagttaat gttgtcaggg taagtagtta ccgtatcaaa 24120 accttgccag taccaacctt ggtcaaactt aaccgaaatc tcaccgctag gagactggat 24180 aacttgtcgg aagccgaggt cgttattacc aataccccat actagagagc ctgctaggat 24240 tgctactgaa ccaattgtag aggctagaat tacttgcgct ttattcatca tattatttag 24300 attcctgttt gtcgttttgt tctttaagtt cgtttgctac agcgtcaatg tctcgtttcc 24360 cactgcggaa agcttccttg ataatgtttc gtccgaagat gaggaacagt gcactcccta 24420 acactatcac tgcccagata attttaagca ttttaatctc cttaacttta tgggactatt 24480 atagcgggtt ttcacccgct atagcaattg ttattttgag atttttgcca cgatgccttc 24540 agcacctttt tctaggttag caaccacttt gtcgatagca tcttcgaaag gaatcgcttt 24600 agatagctcg taacccgctt gagtgatacc agcctgggtt acttggcgaa cgtatgggtt 24660 atcaacacct ttgatttcaa caatcgcttg aaccatttga gcacatagca agtctgacat 24720 gccagatttt agtgctacat ccactgggtt ttccatttta aacagcttca tagcgatttt 24780 ttgcatgaag gttggtttga acatagcgtt caatagtgga cgaattgatt ctttgattag 24840 tttgttagaa gcacgacctg cgttcaagta acctacttgt tttagggctt ctttgtttac 24900 gttgatcatg cctgctttta cttgagttgc tttagtcata tcttctttac cttctagttt 24960 agtttgttct ttctgtgttt taatcattgc tttgtctagc atttctttcg tgatgaacat 25020 gttactatca tcacggcggg tagcccaagg gtttggtttc ttatcgttga tactgccgcc 25080 catccctcga cgacctacgg ttgcccaagt gtctttattt cctggtgtgt gtacatacat 25140 atcgttttct aactctgata cagcctcctg ttccgcaggg gtaatactgc cttcataatc 25200 tgcgtctagc tgaccatgtg tagggtaagt tcctttacct tctagccagc gtgtgcgaat 25260 gtcgcataag ttgctaactt ggtcttgaaa agcgtggaaa tactttttct gatttttgtc 25320 taaagcctcc gttactgctc gtgggaacat aggcattagg tcaataatat ccacccatac 25380 tatacgacct gactctaggt gaacacatct tacagtatca tcattgcggt acagtggaaa 25440 taccagttgg tgcgaagtag ttacaaaagc aaccccttcc atacctttct tagattctgc 25500 ccaaaaatcg aaacgcttat cgaaactaaa cccgtgcttg ctaggtttgt ttttagacca 25560 tgcgtaatac gtggcgtgct taattgggat actgataatc ccacgagact tttcaaagat 25620 tctgtccacc ataccgcctc gtagcatgtc ttggacagtt ttagcttttg gaacggtatt 25680 agctaatgcc agagcaagct cttgcgataa catcttaccg tccatattct tgtttaacat 25740 tttaatcttc cttctcttta atttatgtat atattataga gaaaattaag ctgcttgaca 25800 attattattt taaagttttt atcgcatcag cataaaattc ctgataagac catttatcga 25860 agtccatggc aatagcaagt gttgatttct tgtattcatt gacacattgt tttgtaacta 25920 gaccaaattt atcatcgtgg tctctatcta aagcttcata caagctttct tcaaacaact 25980 gacaagatgt tgctaatcgt tcttgtactt ctatagctaa acttagtcta gctgcaccaa 26040 aaactataaa actaattgca agcatcagcg agaacaccca acctaagtca aactttaact 26100 tcattgagcc tttcatttca ttacctcctc tggatctagt gagtccagtg agtttaacac 26160 ttacccagat acttcaccat cctggtagcc ttctttatac cctcttaaat atgcggctat 26220 caatccggca aagactacca aaacccctat gatagccgta ataccaatcc aactcacttc 26280 ttgtttctcc tgaagaaagt tggcttaggc atcagcttag cattaatggc atgaaatagg 26340 acgtttctat tagtatcatt attttcccaa ttgtctggga acatccaatg ccattgacct 26400 tgtgccaact tcgctcgtag tcgttctaag ttttggctat gggtagtatc taagaactta 26460 ttaatctctg ctactttaat gttttgttgc atatactttt tctcccatag ctttttcgat 26520 ttcaaagtgt tgcgctagct gagtaagttg agtagaagct gcatgtagct ctttaatcac 26580 ttgctcaact aattctgggt tatccatagc taacggttta agttttgcta tggcgttcag 26640 aatatgctca cctgcttttg cgttaataga agtaacattg tacaaggcaa tccctataat 26700 gcgtacgttt tctgcctgca ctagttcttc tactcgtttt agaaaatctt ctctagatat 26760 tagagaatca attgggtctt gtgggattac catcttcata ctagttcctt actataactg 26820 tttttgcctt tataggcgtt tggtctttac cagatgctac agattgtatc tctggaatat 26880 tctggtaaag atatatagta caatcgttag ttttagtaca cgtagcttgg attgctctca 26940 aaggaaacgg tttttcaacc gcctctaacc gtagcttctg tacataggct ccagcaaaga 27000 agctggagac acaaaaacta agcactgcaa atccactcaa aaatatcttc acgttttgcc 27060 accttaccag ttagcccgta catataccca atccaaccac gaccaaatgg gaatttctcg 27120 aactcgtaat gttctaggaa ctcttctttt gttagttcgg tttctgttgg cataatagtg 27180 aattgggttt cattaaacac ttgttttact acttttctaa aagtagaagt cgccatatgc 27240 agttttaaag acttacatat ataggctttg tctagccaac cttcatttgt taggtactct 27300 tcctgaatat gtgcaggaaa agcattacta tcagcatcat aggttggctt gattttttct 27360 tcaagaaatt gttttaattc tactgacacg aaatcccctt aatatcgtat tcatagtcgc 27420 ctacgccacg gaagaactcc caataacccc ataggaaact taacatgtta tttagagcgg 27480 caaagtcacc tgcccttaaa aaggctatac aagctgcgca accaccaaac acggttgctg 27540 ctgtaaaaat gcggtctgtt gttttttgtt ttacggatag aaccattgtg ctttctccat 27600 ttttactaga tgggatgtac cgtcttcttc gaacacttcg aaatggttcg gatgatgaga 27660 ggtagcccaa gtcttacgtt tacaacgaat aaagtcgtca ccaaccttaa catagatata 27720 caaatctact ggtggaagtg tactatttac gtgctttggt acgtttacca cgattagcct 27780 ccatcttttt aactttacgt tcagcgactt ctgcacgagt acgccactca ttacgttcgc 27840 gttctgcctt acgaagttca tcttctaggt ttgcgaacga gttaccacga gcttgcaggt 27900 cgtcgtaaat acgccatagt gcctttttag agagcttatt agtctcttga ggcatatcgt 27960 gcaggttaag catcagacca actgcgtaca ctagagttgg tttatcgtaa tcatttgctg 28020 ctttttcaaa ttcttcttta gccatgctaa tctcctttca aactatgcgt atattatata 28080 aaattttagt aagttaagca aatcaagatt tgcgatttta agcaccaaag tgctcaagta 28140 actcttttaa tccaaccata gttagcttag aaaagtctgc gtcacagtta aatagctgct 28200 taactgctgc aacataaggt gccttagcac gaccagttgg catatctggg cactcggtct 28260 gattctcgtg catagctata agtgctagct taaccttttg catatcaggt aaggttagct 28320 tcattaggca gtccataccg tcgtgaccca ttagctcacg aatatctact aatacatcgg 28380 ctttagtagt tttcttagcc ttagccacgg ttacagaacc gctagacttt ttacctagct 28440 tacgatacat tttggcttta agctttttgg catcttctga gtcagggtaa cgacccatgt 28500 ggataagttc tgctggcttg ataccgaagt actcacattg ctgtgcatag aaacgatcac 28560 tcataaattc cggtgctcca cgtttagtcc aaattgaata ttcatcgaaa tgctcgccta 28620 gtgtatgctt agactgccaa tgtatgttct gctcacccat gtctactgct gtcatatgca 28680 tcttgtctac aatataatat agacgataaa tatctacagc atttaatgtt acgaacgacg 28740 acttatcatc ccaattatca ctagatttaa gccagtctgc tgtagcatta gcaaaaggtg 28800 tacgagtcct tggagtggga tgttgtttcg gagtacgagg tagtgatcca tcacgcataa 28860 tacttgcaat atggtaagta ccaccaaagc gaaactccgc ttcttcgaca agtgcataag 28920 ttaaatccca cagccaataa tagttttcaa gcccttcgta tacccattta gtacatggat 28980 gattttcatg agctttcttg tatggctcac cagatttatt gattgggggt gtattacccc 29040 aacgcatcat agtagttgta agcatttgta caccctcttt catctgagag ataacatgct 29100 tatcacaatg atactgcgca gccacctttg ggtctaaatc taaaacaaaa atattcaaga 29160 caatctcctt tcaaactatg cgtatattat acgaaaattt gagttgttta gcaattacaa 29220 agataaataa cgtaagccag ttgtgtattt gtcaaccaaa tttaatttat ttgtcgctac 29280 tgatactttt aagttaacta gaatatctgc tttcttaact actagggata agtaatttcg 29340 cttggctcta gaccattggg actcccaatc ttctccactt agtttggtta ggtctaatac 29400 gcattgtgct attctatgcc cgaatgtgcg ttggatttct gtaatactca catctgtatc 29460 ttctactata tcatgtagaa gtgcaacagc tctagctagg tacctgtctg ttctggtata 29520 gtgtgggcta tgcattaatt ctataacggt cttctctact tctagaatat gtgtctcaaa 29580 gtaatccgag ccatatgcgt atttttgacc agcatgatgt aacttagccc attcgcgagc 29640 tgtatccact aaatgttcta gtttttcaat tttcataagc tctcctcaca atctatttat 29700 atattatatg aaaatttttg aagtttgaca attcaagaaa ataaaaaaga gagccgaagc 29760 tctcttaatt tacactttag cacgatagtc ccatcgtgct tccacttttc tggtatcaat 29820 atgagtgaat gaacggtaac gtccaatacc atacttacca gggtatgttt ctaatagata 29880 ctctgcaaca gtgtcgggaa atactccagc aactcttaca tcggctgccc taccgaataa 29940 gtgctgagac ttagaagctc ccccaacttt tttgttgtgc tgaacacatc tgcaaccact 30000 atcaataaat actgctttgc ctttaaagtg ttttttaagg cgtttcagta cttgtaatag 30060 ctccgcatct actgtatcat acccacatcc acatttacat gcgaactctt tacgactgaa 30120 cccagtccat agttttccaa aaggtaatga ccatcccata ttacttatat ctccccagtg 30180 cccttgcttg tggattacat accctttcta ggtatgcttc caatagagcc ttttcttttt 30240 tgtccccgta aggttcttct gcccatgcta taccaacgta accagagtag ctatttgctt 30300 ggttaaagat agggcaagtg tacatgtact ttatgcccaa agattcatac tctaagccat 30360 tagtaacgaa tgcccccctc attggtacta ctttttcaaa cagaatagac tggtttctac 30420 ccaccaaatg attttggtaa gtaacacttg atttatctat aactgctttt ttaagtttcg 30480 atgggtctac gttgatttta ccctcccaag ccacaatatc ttggtagtta ttgataaact 30540 tgggagtata ctccgcaata aatacagcat ccgagttagc ttgcacatat agcattgatg 30600 ctttttcctt ggcaacccta ggatattgcg cttctctttc tgcttgtact tgttctataa 30660 cggtggttct tgaaaagttc ttagcaaagt caaagagggt gctctgattg gtaactatta 30720 ggtagctgac caagagaata aaaatagaaa ttactctcat taacagagtt tttggatctt 30780 ttatatcctg caataaagca tatagagagg ctaaaagatt cattttgttc atatataaac 30840 acccccaatt gttattatat aacatacatg tctcaatttc aagacaattc gtagagatgt 30900 taagagtatg tggggagaag tgttatagac ttgtaacaaa taaaaagccg aggcttttga 30960 cctcggctat ataattaata gtgtaatcat taatactgct aatgattttg gtatactcca 31020 atctagtaaa taactactta gagttgctac tagtattaat gaaactgcat gcatgctgca 31080 tatcctaata gaaatgctcg tttctgctca ggtctcatac ccaagcattc catccgtgct 31140 ttcttaggta gtgtagataa tacatgacta actgccgagt caatatcctc tggtacaata 31200 ctagccccac cttttattac attaatggct tctagtattc tctcaacgtc cggagatagt 31260 ctcataggac tcctcctggt acttctacat tcgcgtcgac gttaggatct gtctcagttt 31320 tgtttattagt tgtgacttct tcttcaattg gtccaccgtg gtctctttgt gcagcgtaat 31380 cggtgtctat tgtaaagtta aaagtacagc cactaatcat cagacttaaa aacgctgtct 31440 ttaatatcgc tgagagcttg ttcacgttgt cgctccctta gtgcttgttt gtatagctga 31500 ctacaatcaa gccgtttcgt accactatat agtggtacca ttattcgtac atagacccct 31560 ctatcaggat ctccatactt actgttatcg tcttcttggt atacaccgcc gtctaggtat 31620 gctgatggtg ctatagcgct agagcattga aaaccatctg gagtcctgat agagtcatta 31680 ccatatggta gttctgggct aggtattaat cctgctgcgt taactgttgt agctgtgatt 31740 gtggccaata taatcgcact tttgcacaag ctcttccaac aaccccccat ttttgaccct 31800 ccttaggttt ttcggttaca caaacgtaaa acttctgtaa tttgtctggt ttcaggttat 31860 aaataggtgc tcttaaagac ttgtagctct ccccacctag tacttcagtg ttactataca 31920 ctacgttacc gtctagtgag tcttcatgta cagttacatc aaaatagcgt aaatccacat 31980 cagtattatc taacctgaat ttagctatat acatatcttg atatactaac tcatcaaagt 32040 acatggagc tagggtgagc gcgttagccc accctgatat taagagccct agtagtatta 32100 ctgagcgacg catgatgcta ctacaactgc ggtatagtca ccggctgtgt aatttacat 32160 agatgtacca aacagagaaa tacttagatt atctgtaccg atattttcta ggttatgtgc 32220 agtatcgtta ggtactgggt taactgtagc gttagctcct gttacaccaa atgcagatgt 32280 aaatgtagta ctacctgtgt atgagttagg gcgagtagca aagtcagaag gctttgaaat 32340 actcacttga tacttgttag catcgttgtt gttaacaacc atctcagcag gagtatctgt 32400 agtaaggtta acaccatcca agtgcataat accaggggct gtttgaccta ctgtacagaa 32460 gctttgtact gttgttgaga agttagcgtt agcattgtca gctaatgcag cacctgacat 32520 tcctagagaa gctaggattg ctagtgttgt taatttaatt ttattcatag tattcaccta 32580 tgtttagggg ctttcgcccc tcgtttttac atgtaaagac ctaaagttag ctgtaccgtg 32640 gctacttcgc caaaggaaac agttaatata tttgtaatag tattgtaaga gtaatgtaag 32700 tcgtctacag gctcaataat ccctgaaact gcttctttta ctgcaccaag tactactaag 32760 tccgccccat ctggtacttc gaaaggaata tctatagagc ttctgttaac aacagtctta 32820 gttaaaatta ttggcgcttg taacacatca tcgaatccac ttaaatctag tccagatagt 32880 gggtcatccg ctgttgtagc cagtaattcc ttgaagttga ttttaacgtc aaccattggt 32940 ttgcttgggg acacagattc aatgaaagca ccatcagctt tatcagtaga tgctttaaga 33000 gcgatcctat caccaggagc tactagtagc tcgaaaggct tcatagtata aaccgcctga 33060 gaaccgccag gtatagtgaa ggaagtttcc gattaataaa tcttagactc cgctccgtta 33120 gccgcgataa taacccacca gaaagttgcc actgagttag tacccttagc ttgttcagac 33180 cagatacgta actctgtatt aatagtagcc gtaccttctg ccatgaactt aagcgcaccc 33240 tcaccgccac tagccccaga acccgcaaca gtatttactg tactatctag agtaatgtct 33300 gctctacctt tatggatttt accacaaggc attggaagcc catccgcagc agtattgata 33360 gtatatctaa gcccaccaag tccttggtta tcttggatag tttccatata ctcttcgcta 33420 gctctcaagt gtacttcgtc tagcttattt gattgataca tctcatacat gtagaatgga 33480 tttaactacat ctaattcgaa cttggatacc ttaagagtca ttgggatttc ggcttcaact 33540 ggcgcaacta ttacagcgta gttattagca tcttgtggta ctgtaaacgt cttggttaca 33600 gcgtgtgttc ctgatgtaat atcttctgag atgaacttaa cgtcacttac accccaaccg 33660 tcttcccaaa taggcatacc accaccatct ctggttttat agagttcttt agtgtattta 33720 tctggagccc ctgtccattt taatagatac aagttccatc cgttagtgct attattaacc 33780 tctagtgtaa catctacttg tttaccacgc agagctactg tttccgctgt actgaagaac 33840 ttacctaagc taaagtcaca aatattagag ccatcatcac taatgattaa ttgcccatct 33900 tttacctcag ttgccgcagg atgtggatta taaaaatgca acccatctgc tagagatata 33960 ccctgtccgg ctgctccggt ttgtctttta gagtcatgat ttactacact gtcaaagctc 34020 attctcttct gaccaagcca ttctctagag aactcaataa cttgtctagt atctaactcg 34080 tattgtacta gtgcttgacc tgtcttactg ttttcgtcaa gagcctgaac cataatacct 34140 gagatcctt tagccctctc attaatcatg atattatcag tagtaaatgt atgtactacg 34200 tgcattgtta ggtttgttaa gcctttggca tttagtactc caactacttc caagtcccct 34260 agagtatcgc ctgctttata gtgtttttct gctgctagtg cttgaccgtc gacatctact 34320 acaatatcag aagactcatc gaactgctgt ccctgagcta gatagatgcg aactctacct 34380 tcgtctggcg caacacctgc tagagatatg cgatatgcta ctaggaagtc agccccacct 34440 gtaacgttag ggtctaggtt atcaccttct tggataccca cggttttctg tgctttattg 34500 atagcaatag aagtaccttg actatctacc actaagtcgg taggccatat cttaccctta 34560 gcatggtaat tctctgcttc tggttgtgcg ataatctcaa tagggtagcc aatatatgct 34620 aggtatccgt cagcacgttt agcttcgaat gcgtcgtgct gaatttccat tactacacct 34680 aattcctcat tagggtctga gaacatttgt aatggggaca ttagggatac tttattagct 34740 cttactggaa cgtaggtttg tccgccttcg tcagtagttc cgctagtacg ctcatcagta 34800 taggtagtga atggcgtaac ggacacgcta gtacctgcat cattaatctc ttttacggaa 34860 gagctctcga atgtagcagt atcgacatta tcggtggtaa agaggtcgtc cttaaaggtc 34920 atgccgttac tagaaccacc ttctaatgga atttcattcc agttaccatt agaatactcg 34980 aatagtgctg gtttaccgtt tgcagcagac cttactagta ctgccttatc ttctcgtctt 35040 tcgtcggtaa ttaggtcgcg gtcttggata gcgtctacta cttcaaaacc acctttaccg 35100 tattttgcaa tatgggtagg ccacacatta gagtcatcac gccccggagt aatcggggcg 35160 gtaactggag tacctacagt tattgttgtc atatatattt actccttaat ggtaaattat 35220 tagtgtaacg tcctgctctt ttagtgggta aggagaacgg aacactgtga atgtttcact 35280 ctgtccattg acggtataag ttttagagct tttatcccag aatgcaggta gtccaccttg 35340 ctctccaata cggtcagccc ctgccccttc agcagcagga ataagaatat acgcatattg 35400 agggtcagca gtagatttag taactgtaac cttaccgtta gtaaacttag ttaatgaagt 35460 gaagtctact gtatctggta ctgtagcagc tgcatcaaag aatgcatatg cgctagttgg 35520 gtgtggtaga ggtaacgctc caccagttaa cattagtttt ggtggtgtac catcgcctgg 35580 atcaacaact tccataccaa caagtttcaa tacatctgta actactttac taccatcagg 35640 gttctcgaat gtagctcctt tgaacttaat aatacctttt gtagtatcat cagcaggagt 35700 agtaacatca aaacctttga attccaatga agtagcagct acagcagttt gggtaggatc 35760 atcactctgt acatttaagt tacgaataaa gttattatca gcactcacta acgccttcat 35820 ctcgttaata agatctttgt gggagcgcgg taaaaatcca gagtataatt cctgccagtt 35880 attgtcattt ctaacatacc atgcggcggc ttcaggagct atagatacag atgcctgccc 35940 tgctattgtc tctccttgcg gtgtgtttac tgttacagaa taatcaggtg aattattatc 36000 tatgaataat atagtaccat cactaatctt actagctgtt acaggggttg tgagagttag 36060 ctcagaagtt gtaccataga aagtaaagta gtaccctccg cgagaacctc taggcacatc 36120 ggtaggtata gtatcacctt tatacata aaacttagtt ttgtttttta ggtgtctttc 36180 taagtcagat acagctttat ttagtttggc aatatcgtct acccatgggt tgaacgtaag 36240 ggtagaactg gtatcgtcgt agtagcgtac aagtaagtct ttaccactag atgtagtttc 36300 tagagctttt actacttgcc cctcgttcaa actagaacca ccggtgccac cagaggccag 36360 cactattgca tgccaggagt tgcttgctac gttgcttaca aatagtatgg tatggttggt 36420 aggcacattg aaagaagtaa gtccaggcct attaactata tctgtgttac ttgcttttac 36480 ttctactact gctgcgggtg tagtagtcct attttctact ataaatgcac tatctcgacc 36540 taccggaaga gtacttgtag caggcattgt aagagtaata gaagtatctt cttcaaaact 36600 aaaaatagga gtagagttag tcagttcgga tactgagatg cccctatctg ttaacaactc 36660 tataaagttg tacggtgctg ctttagccga acccccaaca ggtagtgtaa cagtcttact 36720 agtaccatcc agcattgtaa aactgaactg atcaccactt aatgtaatgt catttaactaa 36780 ttttagggtt tttaaccatt cttgagaagc tactatctcg tctgtccact tagctacat 36840 taacgtagct tttggagtgt catactgact acctttaagt ataacgtcag acccatcaga 36900 gctagacacc ctaacatcat aggtattacc tgctttaaag tctgctgggt ttgggaagaa 36960 aatatcctgt tctccggcag ataaatcccc taacctaact aaccataact cttcattatt 37020 taataataca tgcacttcta cgttggtagc atcttgagct acttctattc tagcaccgaa 37080 tagagaggca tcggcaggtg ctgtatagtt gaagaaaggg tttattaaat ctttactgtt 37140 atctgtttct agctctactt ctagtcttgg gccatgggac ctaatatacc ctgttttagt 37200 tccgtagcct acttcttgcc ataaaggagc gtacacttta tttgtaacta cgttagtaaa 37260 ggttacgttt tccgcgctac ttgaaataga atgtgagcct agatgtaatg atgcaggctc 37320 tagggtaatc gagccatctt ctttcgaagt aacacctgaa ttagtaaatc tcttagcagt 37380 cgcgtcccat ttaggaattt gtccttgagt aagatggctg taatcaatac catcgccagg 37440 agcacctgga gcacctttat ctcctttgaa gttagtagca ataggttgcc acgcgttttg 37500 tttatatacg taagctgctg taatttggtt agttgtacca atagcaactg cgtcaccaga 37560 agatttaact ttcgctaatt catctgggtt tgatgtaaaa tatgtgtctc tagcaccagt 37620 gttggcgaat atggcagggt ttccatcttt ctttgtgaaa atgtcaactg caccaccgcc 37680 cctaccagta ccataggctg ggccaaatcc catatatttc tccttaactt aggttttgaa 37740 ctacaactag ctgataagta gctaagtctg ttcctgcaag gcttgatgga gtaactttaa 37800 ctgctgtaac gtttccgccc cattctgcct cagctccagc ttcagtagcg tctagatcag 37860 cattaactac ttcatcataa ttattatttg tgaggtgttt agccatgatg ttcactgagc 37920 cagcactagg gataatttgt gcgtcgttcg agtcaaagta acgaacacca gcgaagcagt 37980 gagcgtactc tggagaaata ggtaactcga taatctctcc tatacccttt ttatcacttg 38040 aaaaaatttg atatgccatt tatttgtttc tccttataaa ctgttttcaa tatttgttaa 38100 tattatagga gaattttaca aaaatgtaaa gaaatgttta ataaatgtta catgaagatt 38160 atttcattca gatacaaaaa agcccctgta tttctacaga ggcttagtct tacttattat 38220 aattggccag tagcgattgc tttggcggat tcttgtaact cttttaatcc tttgtcattt 38280 gtaactacaa tatctacaaa ttcacgagat actccggcct cgctagcatg tccatttgtt 38340 actggacgga atcctggacg ttcaatcaag ataacttgtg cacctaagtc tttgaagtat 38400 tttgcttcat tatcgaaacg aacatctgta ataatagtat tttctttcgg agcaatatct 38460 agccagatgt cgtcatcaat agctctgccc cattctgtac caaatagctg gaatgcacgt 38520 ctagggctaa taatacaata gcctaaatca tcttccttaa tcattatatc aaacagtcct 38580 actagcttac cccaacaatc atgaaattct tcatagtagt ttaaaccgta ggtgttataa 38640 ataataccgg cagcatctag tgtttctggt gagattgaat ataacatctc aatttcttta 38700 taagagccat cacggtgttc ttctccccaa ccaaataaag cacacataat atctttaata 38760 ggttgagcta gggcataggt atccatgttt agttcactag ctagaaagcc agcaaaggta 38820 tcttttcctg aacgagcaaa gcctgcggta cacttatttt tcattaatat tctccttaca 38880 gaatgagtat gagttttcgt aataacaaac aatattttgt aaatctttta tatatctaat 38940 catatccttt ctttccgcat tgacttttac accatctgtg ctagaaagat aaacgttacc 39000 attgctatct actttccaat taacggaata agtttggaca gggcttggtt tattgggtat 39060 gtatttttga agttctatat tttcaggttt aggttcaaca gatatgcagc ctgacaacaa 39120 gagtataggt agtagtaagc gtctcattgt gttaactcct ctagctcctg ctgtgtctct 39180 ttataatctc tatttataat tttttcatat aacttagatt ttcttttagc tatcttctcg 39240 accctatcaa ggtctttaac agcttgttgc tgtattttat ctttttcttg ctgagtacgc 39300 tgagcatcgg cttgataccg ttgagctttt tctataattt ttccattttc gatttgaagc 39360 tgtgtaatgg tagcttcttt ctctttaact ttcaaattcg cagcttctag cttcacttcc 39420 gtagcagcta gctgtttcgt ttggtaatac ccaaaacccc ctgcgcccgc tagcaacatt 39480 aaaagtacac caataattac gtatgtgtta ggcattgttt ataatctcta ctagcttatc 39540 tccatgacaa ggttgtggct tgcaatggca tcctagtctc ttacctttta gagatcttag 39600 atattcatcc gtaatctttc cttgttctct gagagcaggt aagtaatgtt tatggtacaa 39660 ttcgattgct ttcagtctcc caaatgtagc acgagaaaaa ggattaccta gtggtgagcc 39720 tctaccgata tacacgtcac aatcttcatt ctttatatta acaatttttg tcataaattt 39780 gacacctacg aaaaagcccc tcacgattgt gaagggctga atgggtttct aggaattggc 39840 agcccagtac aaagattgga gtcgatagcc tcaacactct ctaaactact ctgggatttt 39900 ataagggtta aagtataaga gtataaaacc taatacttaa tacttaacaa ctaaactttg 39960 aacattgacc gttaatagct gaactttcat ttaaggaaag cggttagacc tgcaagagtc 40020 gaaacgcgtt catacattaa cctctgtaat attcgttagt tggtaagcgg tgtaattata 40080 ctccccaccg ctttgtggga gtgagctagt tacatctttt caccaacccc tagaaaattc 40140 gttattctac ttcgatttca gtagtcgcgt tagctacgga tagctctacg tcaacgttag 40200 ctttgaagtc gtcgatagac tcttcaagct tctcgatttc atcagcaagt ttattagggt 40260 caacaatatc gaacttgttg gcttgttcaa cctgttcttt gatagccttg taagtaccat 40320 cattagcgtc gattttcttc acgttaccga atacttgttc gactttagaa tcaatctgag 40380 attgaaccat ttgctcatgg cgtacttgtt tgttagtaac ttgattaaac tgttgtttta 40440 ggacttgaag aagtgtctcg tccatctcaa ttgaggcttt acgctcaatt gcttctgcaa 40500 cagtcatttc gactccgccg attgtaactt tagttgcagc gttagacgca gtaactttag 40560 ctttgataag agcacgacgt ttcataagag cgcgtacttt atccatgtta gctttaacag 40620 ctttgctagc atcttcagta gttgtgaagc ctgggacatg tccacgggtt gctgttagga 40680 taaactgacc gctttgaact agcttagcga tttgtttgtc aagttttttg atttcagtaa 40740 gagctttagt tactgtcatt ttcataattc tgtttccttc ttcaatttat gtgtatatta 40800 tacttgagat tgacttactt agcaactcaa gtttttagga atttggtact ggcggagaga 40860 ctcgaactct caatcctcta gggcatggga ttttaagtcc cacgtgtatt accaattcca 40920 ccacgccagc gttgttattt attagcccat acaattaggg atgggacagt aaagatagtc 40980 caacgtacta ctgttggcat ccacatgatt ttattacaca tatcttcact tttacaatca 41040 gatagtgcag tacaggttat tccttggaat acaagccata ctacgaatag tgtaaacata 41100 atcttctcca aagatggcac gccctgtagg attcgaacct acgaccacat ccttagaagg 41160 gacgtgctct atccagctga gctaagggcg catttgttta tttaagacca ggtagcttca 41220 ttacagaaag tcatgccttt gtatgcaaaa ccttcatcag cacggaacaa actatttccg 41280 tttgattcac ctaggtatgt aatattgtga aactcaccac gagggaacat agttcgacct 41340 tctttaaaga tggcagtgaa tttttgtttg ttacgtttaa tgattttctt aatcatcgtt 41400 atctccttta gtaaagactt attggggtga cgtacggggt tcgaacccgc atcttccgga 41460 atcacaaccc ggagccattt ccacatcagc caacgtcaca ccaataagtc cttggagggt 41520 agtctcggac tttaaccgag taagacgggg tttgcaatcc cgcgcttcgt tcacttcagc 41580 ttactaccca tttaatttta actaataccc acaagtttcc atacgaatac tttgtctgct 41640 tttccacgag gagttgcatc tgatactttc caaactttct gtaaagtacc tgtaccttgg 41700 ataactacat cttccgtaga agtaggttct ggtagcttat cgaataaact tatagttcta 41760 ggctccttaa tactagcgac ttcctgaact tcatccataa gagcagctag atagccttcc 41820 gagtcgtcac aataatcagc ccagtaactt aactgctcca tcattgcttt aaataggctc 41880 tcactgataa ctacgttttt cataaacaat ctccgtttct caactttatg gaactattat 41940 acagatttct cagcagttta gcaatttagt ttttatcttt ttcagcatca atggctgcgt 42000 ctaggtatgc ttgaagctgt tcttcctgtg gtagtaaagt acccttttct tcttcaatat 42060 catttaaaag agttaatgct acttctgctt gttctagaga taacttaatc aattttgaag 42120 tccttcttaa ccaatgctcg tacatgtgtg tcttcgaact taacaaaagc ctgtacaact 42180 gaaccagcga tggaagtctc aacaggcatg gtcatgccta ggagtttatc ggcaatattg 42240 ccaaaacctt ccaagtctag accgtattct tggagtagat gatgttctac aacttcttcg 42300 tcagtttctt ctggaagacc taaaatgtgt gcagcaactt ctgcccattc ccaacattca 42360 atcatttgat aacctctctc attaatttat ataataatta tacgtgaaat aagagagttt 42420 agcaaataaa actttaaaga aagaggctta cgcctctttt ctccaataat agtatccaat 42480 cagggaattg attaagaata ctagtttaac aacgaatacc gccagtaccg caggttgcaa 42540 agatgcggcg gtaaaccaca taaaagcttg tatcgcgtta taaggaatcc atagcaacca 42600 ttggtctcta taggctccga tcatcaatag agtagctgct actggtaata cagcagtgaa 42660 agcatctaat aaaggtagtg ctccgccagc cccaactgtg aagaagaatg ttacaatcgt 42720 agcggtagtt atagcaacac acatttgcat aaactgattt ttattcaagc tacgctcaag 42780 atgccctgcg cccgttcggc gtttccaata gaaataacca aatatagttg caggaagaag 42840 aaatgctcca ttaaccaggg cgttggcaaa gaaacctgcc ccgaaggaag cataggctag 42900 gaagccacag aaagccgctc ctagtagttg tgattcaggt ttcttaaatg aaacacctaa 42960 tacgaataaa atacctacca ttgatagggt agatacgaat aaatcagccc ccagaaataa 43020 cacatgctag agctactaat aggacagcaa atgctgccca aatcttttga aacaaatcca 43080 ttacacgtta cctttatcta gaatgtatgt aatctcgccc caatcataaa aaccatgacc 43140 gttagtaaca tcaagcacta caccatacat atactggtgt ggcccatagc tgcgtctgtc 43200 tcctgtttct gtaggattac ctgcacgacg aatatctgag ttgtgaacta atacaagctt 43260 attctcgatt tcagtcatgt aagcttcgat ttcttcgata ctagcccctg ctcttatcat 43320 aaacttaaca gttttggcaa cgtctttcat acccttgatt tgacctagct cgatacacgt 43380 accgagtgct gcttcagggg cttccataac cacaatatca gaattcatga taccatcagt 43440 atctgcacga acaatttttt ctgctagacc atcattagag ttgagatttt ctttatcatt 43500 tatatcttta ttatcttgtg gcacgtataa ttcgtaacct agattaagta aatctcgttt 43560 ttcttctgct cgttgaagtt gagcaccacg gtttagcata tcacctgcta gatagatttg 43620 tttcttcata ttttctccac aagaaaggga acgtctagcg ttcccattgt tttcgtttct 43680 tagattggct tttccaacta cgctgtactg ttctacttgg gtaatcccac caagacagat 43740 cgtacatgta tacatctgtc tgtctgaaca agcgtttgtt aaaaccgttt tcaattgcaa 43800 tatcatcgtc tttaagacgt cgatgccagt ttactcttag tttgtgtctg tcccacatat 43860 ttctctcctt agtgcgaccg taacaggagt tacggtttca ggaaagaaat cattaaagta 43920 ataaaacatc ttcgagcatc cttatgagtt ctcgtggact ccctagcgat tactaggata 43980 ttaattggcg gagagagtga gatttgaact cacagaccac gttagcagcc gactccttag 44040 cagggagctc ccttaaccag ttcgggcatc tctccaattt ggcgcgcgat cagggactcg 44100 aaccccagac cttcaccttc gtagggtgct gcttctatcc atctgagcta atcgcgcatt 44160 aaactattgc ttggaactgt gagtaatgat tgtcgtcacg ttccttagcg ttttctagga 44220 ttacgttcat atccttagta catggtttac ctcgataatg ccacttacct tctaagaagg 44280 gtctagtgaa cgttactcct tggtagactg ctgttacatc tttagtgaag tagtcctctt 44340 tatattgtga gaactggcta gtgtacttag acataatata tcctttaaat gtggtgcggg 44400 aagctggaat tgaaccagcg acaccaaggt cttcaaccaa gtgctctacc tactgagcta 44460 ttcccgcttt attctgttac tggagtacgg tacatgactt gatcatgtgt cttctcaact 44520 tctactagac gaccggattt cacaaggcgt tggcactgag agcgcaactt ttcttgagaa 44580 cgcttgtgac ggtttacaac gaatggtttt tcagacgcga actttagtag gttagctgtt 44640 ttcatatagt atctccatga tatttataat tagaattggt gggcggatgc tacgatcccc 44700 tagtttttat tacttagttc gccaactacg attctagtgc gaacaccgcc caagcagggg 44760 gttgtgctat tgcaatatgg tgccccttac tggacttgaa ccagtgaccc tcctgttatg 44820 agcaggacgc tctaaccact gagctaaagg gacttggtac tcccgactgg actcgaacca 44880 gtgacctaac gattatcggt cgtttgctct accaactgag ctacggaagt attgtttggc 44940 agaactggta ggagtcgaac ctacgtaggc gggtttggaa tccgctttgc agccacctgc 45000 tcagccctaa aaatctttac cacacaatgt acaaaagtat tcgggttcgt tatttaccat 45060 aactgaacta cgagcttctc ttggatggtc acaagctgtt tgttgaatag ctcgtaaacg 45120 tgctttttcg ctctctagct ttcttttttg ttctgctagg acttttgctt tgagggtgag 45180 gtcttttaac tcaccgtcaa caatgtcaag ttttgtttta acttctactg gattcataga 45240 acctctcttg caatcttaga cgctacaccg ccgtcatact gaccttgata gttaaaacga 45300 aggaacttca ttacttcacc gatgtttttg aagtcaccag tttcaataat attgcgaagc 45360 tgctcgtcag ttaattgctt aggcaataga gactctagga acacacgctc gcataaatac 45420 ttctcagcgg cttcttggtt gccacgatct aattctagct ggcagttgct tttggcgttg 45480 tctaggtact tacgaataag tgccatacca tcaacttctt taccagtacg tgcttgtttg 45540 tcaaagtcag caataagtga ctgcattact gcacgaccaa ctttgttacc tgctttacgt 45600 acttcaagca atttagcttg catttcatca agttgcatat cttcatcctt tgttattatg 45660 ttaccatttc aataacgctc tctatcaaac cctcgacttc ctagtgttgt ccgtcaacgg 45720 ctcttcccca cagtcactac tctgtatagg gtgcttctat aacctcagca tgacctgagt 45780 cgttatataa cataagaacg aaaatgaaat ggtactccct attggattcg aaccaatgac 45840 cccttccttg taagggaagt gctctacacg ctgagctaag ggagtggcgg gggatgcagg 45900 actcgaacct gcgaccctcg gagtcaaatt ccgatgctct accaactgag ctaatcccct 45960 attgtttatt taactacggt taagaaagca gttttctctg ctggtaaaca gtctttcggt 46020 aaaccacgta cagctacagt tacatctttg tgtaactcct gcgggttttg gactgcttga 46080 agttcatatt cggcatcatc aaaagataca acgccagttt tgcctgcttt atttttaaaa 46140 taaatcatct tcttctccat agtagaaagt ggtgctccct gtccgattcg aacggacgac 46200 ctgctcatta caagtgagct gctctcccaa ctgagctaag gaagctttga ttcgtttctc 46260 aactttatgt aactattata cgttaaataa cgatatttag caatttaata ttttctgaat 46320 taggtcgtca ctcggggtct cttacacccc tagtcgggac tcgaacccgt taccaactac 46380 tgtaccgttg accttagtgc tttatatagt agattcagga atttggagcg tacaggggga 46440 gtcgaacccc agcccctttc ggagcgacag cttggaaggc tgctgaccga ccactcagtc 46500 ttcagtacgc attaattaat ctgtccttcg gagtttcacc gcagccgccc agcatcagga 46560 ctcatacgca tacagagggg aacccgaacc tctgtgctct aattggcggt aggtgtgagg 46620 atcgaactca caaggcgcta ttagcactcg ctggttttca agaccagttc cgtcgccaac 46680 catcggattg acctaccata aatttattgt aacactacag tatcaaggaa tacccacata 46740 taatctttac ctgaacctgt aggcttatta atcttaaaca tggtagtatc agggatgtcg 46800 ctcaagtcta ctctaggatc cactgtaaat atctcagtaa aatctttaac atctaagatt 46860 gtatatacgt tattatcata cctatgggtc tcgcctgctc ggtgtacttc attattatc 46920 gtgcaatgaa ctttctcact agagtagact ctgtataaga tttctgttcc taatttagat 46980 agtttcatat atgtctcttt ttgtttggta caggaggagg gacttgaacc ctcacggtac 47040 aaagaccact ggaatctaaa tccagcgcgg ctaccaatta cgccactcct gcattgtttc 47100 tctttgatgg gttgccctct cgaaagaagg caactcaaag agttagtatt tttctattgt 47160 cttaataaaa tcttctactg tcacattatt gataccgtca atgctgagta taaaattatg 47220 gctaccaaat actgtagcgg catcttgggc tccttttagc gcagccacta acccttttgc 47280 attaacagtt aaaccagtag cctgtctcca catacctggc tcatactgat cggtttggtt 47340 aataggactg ttagcagatc ctgctaggtg tacagtaccc atcatggaat ccataataat 47400 aattaagggc gtgtcagggt cttccgatag caccgctccc aggttttctc ccatagcagt 47460 agttttgtgc ttcaatgcac ggatgtttgc tacttgtaaa taactctcgg tattagaaat 47520 atagtataca gcatttgtca taggactgta gtatgtatgt gtaaggtcta atagaggaaa 47580 gttagaagta cttactcttt ctaggggttc gtctggcatc ccaggtactg atgcagttat 47640 cattcctgag gtgtacagct tgaaggttct ttcaaaacca ttaggctctt gtgctcctaa 47700 cgtagtttct gatactaatt ccatatcccc tgctcccgga tctgttattg ctgtctctgt 47760 acatttttct gcaacattat atgttttagc tatgtcatct acctgaatac taaaacaatc 47820 agtatccaat gataccgtcg catttgccga accaaaagcc attgctgcta cagcagttac 47880 acttaataga attttattca tttaatctcc aattattatt gctggtttag taaattatct 47940 tttgtactgc cccgtgagct gcccttgtca cggtctatag gtatggactt cctagcagtc 48000 aaaagataac tcttttggaa gtgagggttt agatacgctc cacaagccag ttccccacact 48060 ggtcacttct acatattcca caggggtttt tggacttaac cggttcagtg attcgttaca 48120 cgctactggg cgaatgtagg aatatgaaca aacatgtgat tctaaccgct tctgcatatg 48180 ctagacagaa tgctgcacgg gatttgcacg ccccttctac agactaccct attgtgcaaa 48240 gagtagtgtc tctcgtaaaa ttggtcgggg aggtaggatt cgaacctacg acctctcgca 48300 tccaaagcga gccgtctgac cagactgaca ttacaccccg ttaaatcttg tcgataatat 48360 tattgatgac catttttgta agcccttcgg cattgttacc tgcgaactct aatgctgacc 48420 ttacgatttc catttcctct ttagagaaag gattgatgtc agtagagtct tcaaattgat 48480 gctcgaagca cagagtagcg tcttcgtcat acacataagt tgttttagta tgtgtatcaa 48540 tcatagttac gttactaaaa tctagaacct cgtctagtgc ttcactaaga gtttcgaact 48600 cggatacacc ttcgtcgctt acaattactt tgatcatctt cattaccttt ttaaattggc 48660 gcgaccgatg ggacttgaac ccacgtactc ctccgtgaca gggaggttgc ctaaaccatc 48720 tcgcacacag ccgcgtaaat tggcggggaa tgtaggattt gaacctacga ccagctgctt 48780 aacaggcagc cactctgacc gctgagttaa ttcccctaaa tttggtggtt gaactaggac 48840 ttgaacctag atgccccttt cggtggctac ggatttacag tccgctgact taccaattcg 48900 tcgcattcaa ccattgttct tggtagaggc tgagggactc gaacccccgt cttactccgc 48960 atgaaggagt cgctataacc aactcagcca agcctctata atttggtgga gaatatcgga 49020 ctcgaaccga tgacctgagc cttgcaaaga ctctgctctc ccaactgagc taattcccct 49080 taatttggta caccggacgg gactcgaacc cgcaaactcc accttgaaag ggtgacgacc 49140 aatccatctg tcttccggtg tatggtgcgc gttgatggac ttgaaccatc gaccacctga 49200 ttaaaagtca gatgctctat ccaactgagc taaacgcgct taatttggtt gcggtagagg 49260 gagtcgaacc cccaatttcc aggatatgag cctagcgtga tctatatatc cgtttcacta 49320 taccgcaatt atttagtctc tgatacgaaa tccacgttca gcaatttgtt ttgctaatga 49380 tttggtacga cgcaatccta aaccggattt cttactagcc attgaagtgc tagaaccgta 49440 agccaaagag aacattgcaa tcttttgcat tttaccacgt tttgtcatat cttcaattcc 49500 ttatcaattt atggaactat tatatcaagt ttcaaagcac ttagcaagac aatttttaat 49560 ttaagtacca aatgctttca aagttgataa agaggttttc ttcaaagtag ataatcatta 49620 tagcaagaag ttaagcagag agcaattcaa tttttaattt ttatgctctc tgctagtaac 49680 attatgctac gtgagcattt tccgcaggat tgaacgagaa tactcggtca gacttacat 49740 ctttcaacat gttgcggaag ctaccttgag cgtgtgccat agtatgagta actactttac 49800 caccgttagc tgctgtgcgt actcgtttag taccttttac ttctacaagt tcgttagaat 49860 ctacagaggt aagaccacga gcgatgcggc gaataagttt tgcttgttta ccattcatat 49920 taggtatcct tataattagt taataaaaga gagttttaaa ttggtctaat taaagaccga 49980 attgagctgc cgtgtattca accgcacgag ggtggtttga aacgttagta cccttagcca 50040 acagacctag gattttagca agaccggcac gacgttgctc aagcatttta ataccgtatt 50100 cacctgtcat tttacgagta cggtcagcca ttttaatacc aatcatttta gaacgtttag 50160 ccatttctat ttccttcttt tgttttcttc agaatatgtg tatattatat aaagttttta 50220 gcagttgagc aattcaagtt taatcgtttt tgctcatttc ccagatttta atgtaagtcg 50280 cagacaagta gcgaagctcg tgtttacagt cgtctagacc gtaatgagga ataccttgga 50340 aatcttgttc aaatgctttc ttaatacctg gagctaaatc catcaatgta cgtgcatcac 50400 gctctgccca gaattgccaa ggcaaaggaa cttgtgctgt ttcaaataat gaaatagttt 50460 tacctaaatc gaatgtgata ccgttacccc ataccatagg ttctttacta agacctgttt 50520 gttcagccca tgtagctgat actgtcttaa taatatgact tagctgagcc atagccccac 50580 caatttgcag aggtgctggt aagtcatttt tctcacaata ttcagcatca gcaagcaaca 50640 ttttgcgacg agcatcttct gactgcatta gccaaaatga taacgtagaa gctgaaactt 50700 cgcgccctag agcaacttga gaatctacat caatgtggaa cgtagcttca tgaaatactt 50760 caccagaaag aatatcaaat gctactgctg atagctcggg tacaattgca ttctgattag 50820 tcgcatatgt ttctaggtca aatacccagt taaggtgatg atgtgatacc ggactcaatt 50880 ctagtgaatc agggtctaca ttaccataca gtttattgtg tagttcttgt tgcaatacga 50940 tagcttgctc catagttatc tcctgtttgt tagtgaaact tcttttataa acgttatcgc 51000 ttatagaagg agtctcaagc tcctttggct cgaccattcc tgggtgctcg aacccgtcgt 51060 tttcatcttc aaaccactca tccatcaagc tacgcgcgct aggacgatgg tatttggttc 51120 tgtcacgttg gacagttgct ttattgaatg tgcggcaatg ttttgccacg aaattttgtt 51180 gagtcatttc tttactccat cacggtatag acgccagtgt ttccactttt tgttaatttc 51240 ttttctagac atatagtctc ctaaaaacgt aggcagggtc aggtcgaacc ttaacttact 51300 gttataagcc tgccaactac tttattattt gaaacgtgag atgcgagatt ctagaatctg 51360 catatattgc tccattatac ctgcctgttc tactaacagg tctcggtctt gttcttccat 51420 acccttgaaa gcaggattat ggtcgatgaa ctgccacaaa gcgttccact tagtttttag 51480 ctcatcgagt tctgcaacca ctcgttgctc atgaggtcgc attatttctc cgctttaggt 51540 agcggtgcga aacgtggagc aggtagtacg tgacctgctc catcacggta agccttgttc 51600 actttgcgga agttaccttt cacgcgaggt gctttacgct cagtaagctt gccttttgct 51660 acttgctcat cgttaggatg tagcttagcg tggcgagcac gacgagctgc tttattttta 51720 gcagtctgtg ctactttgtg agaacgacga gcttgttttt gagcagtagt tagattctta 51780 gccatgtttt atttccttca atagtttatc agcttgttcg tctaggtctt caggatggtt 51840 gtatccagac caattatcta caccttcatt ttctaggtgc atcaatagta tgtttgaatg 51900 tagtaagtcc cagagtaggg actcatccac aagtacttta gccattacgc tacagtaatc 51960 cactgagcag gcattgcttt tttaccctgc ttagtcttaa tgactgttgg gcgcttatgt 52020 aggcgtactg aataaacatc gccttccgca gaacgaacac gaccatcttc atagacagcg 52080 ttcacttctg aacgttgttc tttacctttc ggcatagaac ggaaaactgg tttaccttta 52140 cggtcaacac ctgactggta tttaccgatt agaagtgagt caccagctac aggttttagg 52200 atttcttttt gttctgtcat attgttttcc ttcttttcaa actatgcgaa tattatataa 52260 aatttttcag tgtttagcaa ttagattttt aatctaacac gttaagttgg agcttcttag 52320 taattggttt tgctccctct tcgtctgaga ggaagaaatg gactaccat ttatttaggt 52380 cgaactcaac aaacgggatt tcttttacac ccagcattgt tgcgtatggc gctgttaaaa 52440 ctacttgttc aaagtacaga taggctgtat caaccgcatc ccaaccttcc caagttttac 52500 aaacaaccgc ttcgaaacca gtggctacta caggctcttt gttcatcttc agtttattag 52560 gcatattact gctccaaatc tagttcatgg tcgaagaagt agtgaacttt gtcactatca 52620 ataggtacac ctttgtgtac ttttaagtca cctagacctt cgattgtttt gtacacaaac 52680 acagggtcgc ctttctgaaa ctctccaaaa ggaacttttg tgatgtatac agtatagtgg 52740 gacttaggtg taacgtcttc tggtaaaatc atttttaatc tccgtttctc aactttatgg 52800 aactattata cgttgtttttt tagtctttag caattaggtt tttaattttc ttgcctagag 52860 gcttgatgta gaagcctact gggtcagttt gagtggtgaa gtatagacga cctatatgcc 52920 cccaaccatc catagttact gtatagcctt ctggcaactt tctcagccat gccaaacgcc 52980 agaatattag gaagttaata ttccaagtaa aattactcat ttcatcacca cattttcgag 53040 ttcccactcg tctattgcac agttacacaa ggtatctgcc aactcgttac cttcatcacc 53100 agagtgacct ttaacttttg tcatggtgac atcaattttc ttggaaatat tatataaaga 53160 cttccaaagt tctaggttct tgactggagt accgcctgct gttacccaac ctttcttaac 53220 ccaaccgtgc atccatgtct tataaccttg gcaagcatag ttagagtcgg ttaggatttc 53280 cacttcggtt aggttagcac gaatcgccca ttctaacgct ttaacaatgg ctgtaagttc 53340 agcctcattg tttgtagtgt gtggcatagc agcactttta gtacctttct tagcccagcc 53400 ttcaaaaaca gcgaaagccc aagcacctac cccagggttg ccacgacatg ctccatcagt 53460 gtaaattttt agcattcttt actccaataa tattgataat aagtaagcca tttttcggta 53520 acggcttacc tgtttttaga tgtgtggttt ggtgcacata aggaatacca cgcactgcta 53580 gccaatcaca catttcttct atttcttctg atgtagcaga tgctagacct acaccacatt 53640 gaagctttgg attctctatt aatttatcct tcagtgcgtc cattaagttc ctctcgaatc 53700 ttcattaata gtttgcctaa gtggttgtgt ccgtgaccag ttttatcact aacaccccaa 53760 aaaccgtcgc cccaccagtt accttcaata agttcggcat cttttgtgcc cattattaaa 53820 gcggcagcta ggtatgggtc ttcgaattta gcacgaagag ctatttccat atactttagc 53880 ttatctttat ggaagtttgg tgtcttgatt gcattaaact tccagtagtt cttagactgg 53940 tttggtggaa gtttggcaat taactttcgc tctcgtcttt ccggcgtttt gaacgcttga 54000 tacaagtttt cactagaagg ataagtaatg ccgtcatgct caatcaccac tggaaacata 54060 ttagagagga acgcatgttc ccctctaaag ctatcaatta ctttcattgc tcttctccca 54120 aacacggtta gctaagtctt ctgcgtatgc tcttagttct agctcagatt ctacgtatac 54180 atataggagg taacgaccaa taccgctagg gtttgtgata gatagctcaa atacacatcc 54240 acgattaaaa cctgctgagt ataggcggga tagaatatcg tcattagcca tattatcttc 54300 gtaacactca tctagaatat ccgccaattg cttggttttg tcagtaatga caatttgatt 54360 ttggcaacca tctaaatcgc gatttaggtc gtctacttcg ctatccatac gtccagtaaa 54420 tagagaaccc atatggtaca ttttctcaat aagaatttgg ttattttcgc aaaattcgta 54480 gtctaaaaac atatttaatc tccgttttca atttatgaga taattatacg gaaattcgct 54540 cacaaaagca aatgaatttt cgaataaaaa aggagggctt acgccgctcc taagtaaatg 54600 atattttggt tgctacttcc acggaattta agacctgcat catacaagtc tttctcaaac 54660 ttaccatcta ctagaacatc aatgtagtct agtatagcac aaaaccgacc ctgttttact 54720 tgctctagtg tgtaaccggt ataaagccaa atatccttag ttggacattc ctttctaact 54780 cgcttacaca agcgtaaaac cgatgcaaaa ttccgtttat gaagtggatc tcctccggat 54840 agagttaatc ctctacgttt gatacgagta tcctgcaaat ctttaataat ttggtcttct 54900 agttcttgag taaattcatg cccgctatta ggattccagg ctgcttcatt atagcagcca 54960 ttacaaccgt gtgagcaccc agacacgaag aggacgcatc gcgtcccctc tccatttact 55020 acgtctgttg ggtagtactg catgtaattc atattagccc tcacaagcta cacattcacc 55080 tttagatgct tgaacacctg ccatagtgcg catgtagtat agttgtttaa tattttcgtt 55140 agtaaatgct tcttggtgta cttctaggat gtattcttca tcttcgtcag catcgaagaa 55200 taagtttaga gactgacctt gacagatacg tggttgacga gctgctgcta aacgaataat 55260 agctttctgg tcaatttcat atgctgtacg gaataccatt ttctcatggt ctgataacca 55320 atccaaatgc tgtacagaac ctagcttatc gttaatagat ttgataagtt cacggtctac 55380 ttctacacct ttctctttac agaatttcaa gaatgatggg ttaagacgag ttaactcacc 55440 agcagcactt ggttgtacat agctgttgcc tggtagtggt tcgataccct gagatacacc 55500 accacatact agagcactag aagtatttgg agcaactgct aatctatggg tattacgaac 55560 accatagccc ttacaccatt ctggttctcc cataacttca gccatccact tagatgcacg 55620 tagtgcttca ttggacatat gagtaaagat ctctaggttc ttcatatgag cttcgaaagt 55680 ctcgaaagca atcatattat cttgtaggta agtatggaaa cctaaaccac ctagacctaa 55740 tgcgcgagat ttttcggtga aacgaatagc tttctctagt cctggaatac ctcgtgcttg 55800 ttcaataaat tcctgagcaa cacaatccaa gaatactgta gctacgaata ctgcatcagt 55860 atctttccac tcatcataac gtgctaggtt catagaactt agtacacatg taaatgtatg 55920 gtctgggtca ctgaacaggt tgatttcagt gcacaaatta cttgcccgta catctagacc 55980 tagatctttg tacatttgtg gagactggcg attaacctta tcagggaaga agtagtagcc 56040 tttacctgta atcagcttag tgtgcagaga cttagctaga cgatcgattg catcttcatt 56100 accagcttct agttttctta cgaactcatc cgagatattc caacctaggt taaggtcatc 56160 tggatagttc ttcagatagg taacaacctc atagaagtca ccgtgatcca ttggaagata 56220 acccgcccaa gcaccacgac gtgtagcacc ttgagccaca tcacgcatac attgtacata 56280 atcttcgatt acaggcataa cacctgctgc tgtaccacca attgagatac tagcaccacg 56340 aggacgaata tcaccaagat agcccgatgt accaaaacca ttcttagtta gcattgctgt 56400 ttcatgtaat gtgtcgtaga aaccatgaat agagtcgtca atatatccgc cagaacaact 56460 aacaggcata cctttatttg tacccatatt tgctaataca ggagttgatg ctgctaacca 56520 acccttccaa aataattcga agaaacgttc ttcccattgt tcttgtctgg acggcatgtg 56580 ttttgctgct gttgctgcaa tagtacggta acgatcccat actgtctcgc cagctagata 56640 tttttgtttt aatagctgaa agcccgcagt agtcatccac tcaggaatag taccgtttgc 56700 ttgaccttct tttcgttcag cacctaattt ttcgtaaata cttgacatta tgctttcacc 56760 ttccatctta gtcgtgattc atcccaatca cggttatatt ctgagcctgt acctgtaaag 56820 aaatcgtgga atttcagtgc gttaatattg tcgtagaacc actctgcaat tgggttatta 56880 ccaatttcaa agattggagc aatacgtaag tgacctagac ataggttgat acgagactta 56940 acgaatgttt taaggtcttc tgcatggaag ccttcaattt cgcctttttc gaacatcata 57000 tcaataatac gtgcttcgtg ttcgtatact tgacgacctg cactttgtag tcgcttagct 57060 actttattga attttgagaa tgtaagctcg ccttcatgct ccaattcacg gcgtaaagta 57120 ttgaatgccc aagctccacc ttcacagtgt aggttttcat cacgtactga gaagttaata 57180 ccacgaacga tatttaggat tttattctta ccttgtgctt ggaagtgttt taggtatgcg 57240 aatgcactat atagtacagc accttcaatc atactaaatg caccaacaga tactagatcg 57300 tctgggtcac atactagaga ctctacaaat tccatacgag acttcaagat tggatcgtct 57360 acataactag tgtagaattc gtccgtattt agcatcagag cttcattaat cttgttgtag 57420 aaaggagcat gaatgttgat ttctgcgaat gagaacgagt tacccatctg acggaaatca 57480 tgacgaggga acatgcgttt aaaacgaccg ccccaatatt cattacctaa tactagctca 57540 tacagagtaa atagtttcaa ggtggtaata acaccgtgag actctgctgg tgtcatatta 57600 acacgaatat cttgaatgtc tttttctaca ttgatttcat ggtggaacca aataactgag 57660 ttttgggcat ctgcgaatgc aactgcctct ggataatcga aggtataggc ttctttaaat 57720 gtctgacaac gtaccggcat tataaagtct ccaagtatcg cataatctcc agtgattcta 57780 acattactgt atcatcatcc aatagtaaag ctggaatctg tgtgattcta tgttttgatg 57840 ctaattcgaa tgagtggtca atatccacta gttgcacatc cagtcctttt tcttttatta 57900 gctcttttac tggttggcag tacttacacc atgatgccat aaataatttc atattctttc 57960 tcctagggtt aaaaagaggg caattgccct ctaaagatgt tttacacgtt tagtcatttc 58020 tgactgttta cctgcattga atggacgagt accgggatta cctaggtagc cacatacacg 58080 tctagtgaca gagcattttt cagggtcgtg gtttccgcag ttaggacact caaaaccttt 58140 gcttgtacag ttgaactcac ctttgaatcc gcagctatag cattcatcaa ttggtgtgtt 58200 ggtgccgtag taggggactt tggtgtaggc gtaatcccac acattttcga gtgcttcaat 58260 attgtggcgc atattaggga actccccgta gcagatatga ccaccggagc tgtatgggat 58320 gaattgcgct tcaaagtcga ttttatcata cgggtttacc ttcttcagta cgtccaagtg 58380 gaatgagttt gttaagtaac ctttatcagt taccccctca aactcaccat actcacgcac 58440 aatagcctta cagaatcggt tacataaaga ttcgctagga gtggcataga ttgaataacc 58500 aacaccttcc gcttctttcc attctttagc tttcttgtca aggaacttga caatatctag 58560 aacgaactgt tgcttttcag gctcatcgta ggtatgaatt acactaccta gcatagcatt 58620 agcagcctct tctaggccaa tatagcctaa agagatgcta gctccgcgct tcaacaggtg 58680 tggaagaacg tactcttctg gttctaggtg taagcctagc gctccttcca tatacataat 58740 cggcgcggat ttagctttta cggttgacaa acgttctaga cggtattgta aggcacgatg 58800 tgcaatctca caatagttgt ctagcaatgt ccagaactta tcgtaatcta gttgagcttc 58860 tagcgcaatc ttaggaatgt tcagagaaac cacaccaagg ttattacgac ctgcatatgt 58920 ttctttaccg tcttcatcat accaagtaga caagaatgag cgacagccca taggtgcctt 58980 atatccgcct gtaatttcaa cagtcttatc gtagttaaga atgtctggat acatgcgaac 59040 agtagcacat tctaatgcaa gctgcttaat atcgtagtta gggtcttctg gcttcaggtt 59100 tacaccgtct ttgagaccaa atactagctt agggaatacc ggagtagtac cttctttacc 59160 tagaccagca atacgattct gtagaatacc tttctgaact agtttagctt cccaagaagt 59220 acctagaccg aatccaaatg tcaagaacgg agtctgacca ttagttgagt aaatagtatt 59280 tacttggtat tctagagtct gacaagcatc gaatacgtct ttttcagtct tttctttagc 59340 taattcaatt actttgctag agtcttcacc accaaagtat cctgctagtt cgctcattag 59400 acgaatgttt ttctcataag tcttagttac gtaaggtgct agaacttcat ccaaacgatc 59460 aaaactagta ccgccatact gtgaacttgc taccgctgct gcgatttgag acacaactgt 59520 ggctgctgtt tgaatagact taggctgttc aatacgagca ttacccattt taaacccgtt 59580 agacaacata ccttccaagt ctactagaca gcagttagtc atacccatca tagggaagta 59640 gtctaggtcg tggaaatgga tgttacctgc tatgtgttct tgtgcaactt ctcgtggtaa 59700 taggtaattc aatgcgtagt ggcgagaaat ctcacctgct aataggtcac gttgtacgtg 59760 aaatacagtt gattctttat ttgcattttc atgcagaata tcagggttag tttggtctac 59820 caaaccaaca atgtttttgt gcaggtcact ttctaaatct cgcgcctgct ggcgatcatg 59880 acggtactcg atgtatttac gtgctacatc tttccaacaa ctagacataa ggacgttctc 59940 aaccaaggtt tcaatctcgt atacggttgc ttcttccttg ccctgctctt gcaggttatg 60000 aatgactagt tgagtagcgt catttgcaat ttgactggct tcttcatact cacaatcagc 60060 atcactagca gacataaaga ctgcctcata aatcttgtgc aggttgaaag cctgtcgtgt 60120 attatctctt ttaattacaa tcatacttct cctccgaggg acggaaatgt catttaaaat 60180 tcccaaactg gacatatatt atagcaaaat acttgatcta tatcaaataa aattttaaag 60240 tttctgctat gagttttgtt gtaaaattta agttgacaag tttccctaaa atatggtaat 60300 tgacatcata cttatattat gggcgaacga gtttggcagt aaaacaaaaa tgtatttgcc 60360 aaactgcccc aatttgtcat ataatgtatg cagtttcaga aatgtaggaa agttttaatg 60420 aatatgcagg ataaagtacg ttatttgacg gagatattag aacacctggt atctcacgtc 60480 tcggttacag cggtagaacc taacggtcgc ccacacaata tccgtcagtg caatctacag 60540 gagaaagtag acttactggc agagttcccc gaaataaaag ttagtgcaga agtctattat 60600 gatggaaaag tacatgatgt gcatcatatc gttcgcccta ataattataa gcacttcgtg 60660 accggccaag gcaaacaaat cgctaaagag ttaaagatgg aactcggttg tagtatgctt 60720 ggaatcagtt agcgtgtaac atctaaagtt tttgatttga cattgttaac caaatgttat 60780 aaaatatgtg tattgattcg aaagagtcga tttccatatt acgcttctcc tggggtagtg 60840 ccctctaccc cgttatcttt atgggcattc tatgagtgtc tataaacata actcgcactg 60900 cgtgtgacgc tcaagcacgt aggtacacac ccgaatctaa aagatgaaac gctagtttta 60960 ctagtaccct gagaatggaa gtaaccggca atgtagtgtc tgtcaaagat agggttataa 61020 attgtacagt ggagtagagg tagtgaccta gcccaggatc ctcttgtggt aggcaacagt 61080 catgaaacta caaagctcgg cgtaagagtg aactattatt ggaactaagc gtctatagtg 61140 taatcagggg agagctggct atagacaact tgccgccatc aaaggattgg tggatgccgt 61200 gagccaggct agcagtgtgt cctgcatagg agcggggaat cgccactcac atagataagt 61260 cccatttgag cccttcgtag tgttgaaacg ctgagatacg tagctataag ttagggtagg 61320 tggattactg tcggcattag catgtaaaag taataagcac tataaacaaa atgttcaacc 61380 aacatgggag atatttaatg ctaacgttta tcgaaggcgt atttaagcct tacttttcag 61440 gtctggagta tattcaacct gacccgaatg cgaaagttcg tttagcaaaa gtttttgaca 61500 aagatggtaa atctaaagac cctgttatgc ctaaattctt taacgtaata gagtcagtta 61560 atcccaactc actagaacaa gtacataagc taatttctga gtatcaaaga aatccggaga 61620 ttgctcttgt acgtgcaaca ccagtgtcta gtatccgaaa tatacgacga aacgccgaga 61680 cattcaattg ctcggttcgt tcgcgtataa ttcacatgga tattgatggt atcgaggctc 61740 ctagagccaa tatgaccata gaagaacaag ggcgatggtg cgtagaacta ttgaatgagt 61800 tagagccaga aatgtttcca aaaacagcag cttacatagc gaaagctagt ggtagtgccg 61860 gaattaagcc tggcatccgt ttgcacatgt atatgcaagc aactagggca gtatctaatg 61920 cacagctaaa atggttagcc tatcagatta accgtaaatg taaggaatta cacgattttg 61980 agcttttaga tacgtctgtc tatgataaag tgcacttgat gtatactgct gctccagttt 62040 ttgagaatcc tgaattagac cctttcaagg ataaagaaag ggcggtttta gtcggcgggg 62100 ataaggtgga tttactagaa actatgccag agtaccaagg tatggcgtct tataccttga 62160 agaaagaaca cttcggatat attatggata ttgaaggatt ccatgattat cctagtaaag 62220 attttgaaaa gcgtattgaa aacctaaaaa cggcaaagga taacgtgttc atgcgacaca 62280 gtatctctgt ttatgcagca gccgtagaac aaggtgtaga tattaattgg ctagatgaac 62340 aggttcgtaa tatcctcaaa gggtataatg gtttgaaacg tgaaccagaa gcctatatca 62400 ataacgctaa ggaagcagca cttaaaatcg ttctatctcg tagtttacgt aaagtaaaag 62460 gcgatgttaa tgtttcaact gaccctacag ctccatgtat gttgcctgta aaggatattg 62520 aaacagatag taacgtaaat gataggtttt tgaaaattaa ccatttacct ccagaggata 62580 gtttgacatt tgttaaagct agtttgggta caggtaaaac aactacagta cagacatggt 62640 tgtctagtgg gttgtttgat ggtcgttttc tatctattac taacaccgta tctctagtag 62700 agggtaacgc taagaaactt gagtctggat gttataataa actgaaagat tacaccgagt 62760 tccgtgatgg taagattaat cgtatgtcta ctactatcca ctctttgcat cgcttctacg 62820 atacaatcaa tcaaaaaggt ttaggcatgg tctttataga tgagtgtgat gctgtgatga 62880 atgatattct attcagtgat cttatcaaag aaaaagacaa atgtattaaa accctaaaac 62940 taatcttgca agaagctaag tatgttgtac tatcggatgg tgatatttca ccagaaacta 63000 ttgaagcata tgctaggcta tgtgaccctg taaaaccagt aatgatttat cagcatgaca 63060 gaaagatgtt agctaacgct aaagctatcg aactgtttga tgaagcatct gtttgggctg 63120 ctatgcaagg cgcgttagag attggtgaaa aatgcttact agtatcggac tgctcaccag 63180 atgagcttaa cgagaaaggt atagccttac ggagtgtaac tgctgctaat atcaaagaaa 63240 tccacaaaaa ctctaccaaa gatgcagata tcaaagaaat tctcacgtat ggaaaccctt 63300 ctctacaaca acagagagtc gatggtcttc tatgcagtcc ctctgttact agtggggtgg 63360 actttagcta ttttgatact gtctttctaa taactcgtga ttcgggtatc cactccccaa 63420 acctgcgctt ccaggcactt agacgtgacc gtggagctaa aactatatac tactataccg 63480 ctccacagac ggaagggttt aaagcaggcg cagataagta tgaagaatca cttggctggc 63540 tacaacgttg tcgtaaaatc tttgctaaac gacgagaaga agaatgtaca aagtataaat 63600 caaccttccg tatgttatta cgtgaccaag gttgtcgaat ctccctagac cctagtaaat 63660 ggggtaacat tgaatcagct aacgcagaat accatgaaga acgtgtgaac gcgattctat 63720 cagcaacacc tagtttccag ttacagcgtc acaacgatgc ttgggaagtg aaacagttta 63780 ttacacgtta ttatgatgat gtggatgacc taggtgatgt tacagaagaa atggtagaac 63840 gttggttgaa agagaagcct catgatcgtg cggcattctt ccataaagta cataaacgtt 63900 tctggaagat tatcaaatca tgtacccata actttgagcc ctttgttcag catttgaaac 63960 agttccctag tgactggtat caatgcacgg gtctagatgt tcgtacagag ccttggcgta 64020 ttaaacgata ctttaaaatg atgggtattg aagagcctgg tgagtttgat aatattatca 64080 gttggtatcg tacttactgt aaaatcgagg gcatccagat tccaactgag tttatgactg 64140 agttcgaact gtcaatgcta ggagataaag acgtattgtt actgtaggga ttgaaagccc 64200 tactttttta cgccctaaca aactaatagg taagatatgg ataaggtaga atcaatagca 64260 agagttgaga atctactaaa aagtagaaaa tactctgaaa aagaacgtaa ccacattata 64320 gatctcacaa ataaaaggtg gggtactatt ttcgataagt cccgtaataa attccaagag 64380 gaatttacga tacaccaatt cggaataata cttagtagga attactatga tgtagttaat 64440 agaagtgaga gggaacagta tgctatgtag ggttgttcca ccaatagttg ctagacatcc 64500 tgagatactc aactttccaa aacccgtcag gacaactaca agacctgcga tatattataa 64560 aaagattgag aaaagaatct catctctagt cgaggataag ttatgtggaa gatattaata 64620 ttcaacgtaa tagtagggct tctttgtgcc gctgcctata attactcaca acacggagat 64680 attggattag attggtcttt tttagagcaa gagattcatc aaattgaaaa ttaatttgca 64740 aagttacagg gaagtacgta taataacttt agaaattgag aacaaaggag atttaacatg 64800 cgtttcatca gatgtatagc ttgtaataaa atagctatgg agatagaaga aggtcgtata 64860 gctaaaggtt gtaagattcg ttgcgctaag tgcgaagaaa cattaaaaaa ggatttgcag 64920 aacttacgct ttattgctaa aatgtatgaa aataaaaaag caaatccttt aggtgatttg 64980 tttggtaaaa atggttttgg attataggag ctaatcatgg ctagacgtca acgtgcggat 65040 attactgatg aacaattcaa acaagctatt gcttggttag ataatggcgg cactaagaaa 65100 ggtgcttgtg agattctagg tgttggtaat aacaaaacta tggaatcacg tatccaagaa 65160 tggaaagata atcaagctac ctctgctcgt ttgcgtaaag agaagcgtaa acaggcttgt 65220 actccgcaag agcttgttaa tatgattgaa gattactttg atggttcttc ttttgatgaa 65280 ctatcaaaac gcttctatcg ttctacagca tacattaaac acaaattaga actagcaggc 65340 gctcttatcc gtgaccgtgg tgagcgtaac atccttaacc caccactgtt gccagaagaa 65400 tgtgttcttt tagaccctga tttccgttgc cgtgagcgtg taagttttga ggccgcttct 65460 ctagctgagt ttgaagcaca aaagaaacgt atcatgtcag aaaaaggctg gaaacagtcg 65520 gatgttattg atgtacgtac tcaagcaggt aagtgggaac cacattgtgc tcttgaccat 65580 aaaggtgaag tagtttggct acctggatac caatgtcttg cggaagttat cagcgaaatt 65640 cctagcaaac aaggtaaagc ttacaaacta tacctactag accctgaccg tcaccagtat 65700 gttaacatta tgtactggga tattggctct ctacgtcact tagaattgct aggagttaat 65760 atatctagtc gtggtacgtt cctaaatggc acatcctgtg ttgagctact aaacaaagcc 65820 ttgctagcag cacgtaaaag taagtaaaaa ttgaattgct aatctgctaa aaactatata 65880 taatataatc ttaaagttaa acaaaaccaa aaggaaacaa tatttatgac tacaccaaag 65940 tggactgatg aactacgtaa cgaagctgtt gaaatgtacc ttgagcgtat cgctgagtat 66000 gatgaagcag accgagctgc aaactctaca gaagtatgta aaggcattgc agatgagcta 66060 ggcttctcag ttaactctgt tcgtgctatt ctacaacgtg caactgacga agaaggcaac 66120 caagtttatg taaaagcaac taaagctcca aaagctaaag cagcatcaac tggcggtaag 66180 aaaatgtcta aagctgacgc gcaagcagag ctagtatcag cacttcaaga tggtggcgca 66240 gaagtaaacg atgaactaat ggaagttatc gaaaagctta ctggtaaagc tgctcaagca 66300 ctagcaggtg caatccgtac tatgggcgac gagtaatcgt cccctctctt ctaaattaac 66360 ataggatagt tgctatgaaa ttaaatcata ttcttgaaca ggcggaaaaa cacggtgatt 66420 tctatatgta ttaccgtaaa aatactggca aaggtacaac ttacctagtc ggtactagcg 66480 atttcgacaa taaatacatc caagcaaaac aggctactgg taaagcaggt gttacagcac 66540 atgtatcatt agagctattg caagcacaag ctaataaaca ggacaacgtt ttagtcttta 66600 gttggactaa cgataagttc cgtattttaa gtgctaaaga tgttcgtcgt atgtctccgt 66660 tagcagcgga gttacgcaat ggacgtaacc gataattaca atggacatga tgacctctta 66720 gatgaggtca tctatgaaag ttatgaaaag gacacacaga ttcgccttac agtctcagaa 66780 tttagaggaa atctgtatct tggtatgcga aagtgggtga tagacataga cgactcatgg 66840 atgccaacta aacaaggatt taccttccct tataatctaa atactacaag tgcgctattc 66900 ggggcgttaa ctgagattct aagtaaaggc gaagtgttac atgaagtttt agataaactt 66960 aactatgagc cgaatcgtaa atgattcggc ttttttattt tctaggagaa aatatgaacg 67020 tgaattccac tactattgga gccacaaatg gcgcaataag aatattcata gacaatgaag 67080 aaatttatgg ggttataaac cttaatacta cagaaaagta tatagaacgc tacgtaaaag 67140 atgacaaagg tcattttatt gtagacggtg gtgaactacg tgccgaatgt gtaccatttg 67200 atactgctcg cgtagagttc gacaatgttg ttatagaggt gaactaatga ataaatttaa 67260 atgcgtagca tcctctatac ggccagatag aaatggtaac gcttttacac tagaatctat 67320 caaaaagttc gaaaacttag aaaaacctat tttagtaaac tatgaccaaa gtagggtagt 67380 aggcagagta acagatgctg aagtagtaga cggtaaatta attgtgtctg gagagttagt 67440 tgtagatatt actgacttat ttgtagcacc gtccttccgc tctatagatg catacgtaga 67500 acccgactct gatacactag tacataccaa tattgaagta atgagctatg gcctaaccac 67560 ctcgcactca gactatgaag caaccccagt agagttttta aaggaagact aatgaaatat 67620 agtaaaccaa aatcaatcgc agaacgtcaa caatcattcc accaacgagc taaacgagca 67680 gcggatgatc tagcagaaga aaagtcacat atcttaatgc tagaggaagg agagagctat 67740 gagactatga tggctcaaat tagagtattc ctatgtacta tgtatgggtt ctcagctaat 67800 gggtctgctg aattaattga aaaacaccta caagtatcaa ttcctcaaaa atgggcttgc 67860 gtttcaggtc gaatgtaagt ataatatatt cataaattaa aggagatttt aatgaaagaa 67920 tttattaaag aactacaacg cgcgtactac gatggtgagc ctttaatctc cgatgaagag 67980 tatgatgctc ttattcgtcg cttcccagac gcagaagtaa ctattggtca taagggcgaa 68040 gctgcacaca tcttccgtat gtggtcgcta gagaagaaat atccttgtcg tggtgacgaa 68100 ttaccaaacc tagaacctta cattgagtct gataaacttg atggatgtgc tgctgattgt 68160 ttatatatta atggtaagtt tgttcagggc ttgactcgtg gtgacggtgt aaaaggtcgt 68220 gatattaccc aaaacctcaa agaactagta ccagcagaag taaactatta cgctcctatt 68280 atgcaagtaa caggcgaagt agtaactact aaagatattg ataacgcccg taacttcgca 68340 tcaggcgcta tgaacttaga taaaggttct gacttcatgt ctcgtcttgt agaaggcggt 68400 ttacgcttta ttgcatataa tgtacaaact agctctgata gttgtttagg tgctatgtac 68460 gacaacgata tgaaagagct agaaagtctt ggcttccata ctattcttag cgataatgtt 68520 aagaaagctg tagaacaagg cttaattatg actgatggtt atgtattccg tctacgtcgt 68580 aaccgcgact acttcaaggc aggttttact tctaaattcc ctaaaggtgc attcgctgtt 68640 aaggaagacg atgaaggtga aatcacagag attgaagatg ttctatggca agtaggcgct 68700 tctggtaaag taactccggt tgctattgtt aaagaagttg ttctcgaaga cgcaaaagta 68760 actcgtgtta cattaaataa cgtagaatat atgaaagcta tgggtattac tcatatcggt 68820 cagaaagttc gtgttattcg tgctggtggt atcatcccta aaattgtaga ggcttttgat 68880 gataattaaa gggctagaag taattataca ataccccgta aaagtgagaa agttcactag 68940 atggggtata tcaaaatcca aagtaggtag aagatacgta aagctacaca gagtaactta 69000 cgaaaattcc atacctgatg atcaagctat aatatataaa gacaagctta ttgtaacacc 69060 aaaaatgtac gaggcattga agaatgaaat taaatctaaa gaaccacagg gcttgtgtgg 69120 tgggttcccg aaccttaaca gagcaggatg cggagcgtat ccaggttatt ggtaaactac 69180 tattcttact aggagtaaca ggagcatctg gtaatgctct aggcaatgat aaagagtggg 69240 ataagtatat cttcgtccag cacttcttac cttggaatgg ctataatgat ggttatgaaa 69300 cgggtaatgc acagttccta tcgctagaaa gctgtccaga agcgttacga atggaggctg 69360 ctagaattgc agaagaccac caccctgcgt gggatcgtct aaaacgcggt ggtcgtgcta 69420 tgcatacacg aaatgtattc caagctttag gcgttgctct aagtcctgag actatggcag 69480 acctgactgt ttactgtgct gaagaatcac aagcaagaat cgttaagggc ggtactagaa 69540 ctgctgtgga aatctcccgt agttatggta ttcctacttt taacttgcga tttgacaagg 69600 acttttacga attaaaagcc actctcgaag agatgttaga aaactgattt gacaattttc 69660 ataaatcagt atatactagt catcacgaat tgagggaaag ctaattaatt aaattaccat 69720 ttagcaaaaa gattaaaaac ttaattgctc aactgctaaa attcttgata taatattatc 69780 ataaagttga gggagataat taaatgcaaa tagtgattcc aacagaatgt ccgtcatgcg 69840 gttcaactct tgaaaacgtt aacggtcaat tattctgtcg aaataaaact gcttgtcccg 69900 cacagagttc taaacttgtt gagaactact gtaagaaaat gaaaattaag ggtttcggct 69960 ctgcaactat taccagacta gaactatcta ctatatcggg actttacagc ctgacagagc 70020 agcggctcac atcagttctt ggtgataaag taggttcgaa gctctttacc gaactacaaa 70080 aaactaaaac cagcgatttt gctactgttc ttggctcgct aggtattaag ttaatcggta 70140 aagtagccgc tgagaagctt gctacaaaaa tcaatagttt tcaggaaatc actgttaaag 70200 cctgtaagga tgcaggtcta ggtgagaaag ctacggagtc cctcatggaa tggtgtaaat 70260 cactagaggg tatggataca atcgaagctc tggacggttt tattactttc tcggaagtaa 70320 gctcaaaacc gacacaaaat acagagcaca aattcgatgt atgtattaca ggcaagctta 70380 acgacttcgc tagtcgctca aaagctgctg actacttatc taaatacggt atctctgtta 70440 aaggcagtgt taccaaatca gttaaatatc tcatttgtga agatgatacg aagaaaggat 70500 cttcgtctta caagaaagca ttagcaaatg agttacctat cgtatcaatc aaagaattaa 70560 ttaatattat cggagaataa aataatggct aaattcgctt ggaatgaaga aaacgtatct 70620 gtacttaaag ctcgtgtaga aggtgttact ctagtatcac aagaattgct acaagacatt 70680 gctactgagc taggtcacac tgctcgtggt atcggttcta aactacgtca acttgtaaaa 70740 gttggtgaac tatctctaga agttcaaaaa gcatctgacg ctaacaaatc agcatgggct 70800 gaagacgaag aagctgctct agttgacttc ctaaacactc acaacggtac aatgacttac 70860 aatgaagttg ctgctacttt cctaggtggt aaattcactg ctaagcaaat tcaaggtaag 70920 gttctttctc tagaaatgac tgacgctgtg aagaaagctg aaaaagttgc tgctaaacgt 70980 acttatactg aagcagaaga agcaaaattc atcgaaatgg ctaacgctgg tgactctcta 71040 gaagcaatcg cagaagctat gggtcgtcca ctacaatctg tacgcggtaa aggtctaagc 71100 ctacatcgtg aaggtcgtat caacgaaatc cctcatcaag cagaaagcaa agctacagta 71160 cgtgctgact ggctagagcc tgtaatgggt gaaatggctg acctaactgt tgctcaaatc 71220 gctgagaaag ttggtaagtc agaacgcggt gttaaaaacg ctctaactcg tcgtggttta 71280 gcttgtgcag accataacgg tgaaaaacaa cgtgcaaaac tagacgctaa agctgaaaaa 71340 gctgagtaat tcttattaag tgaaaaagag ggaggctatt agcttccctt ttttattatc 71400 ggagcagtaa tgataaacgt tcaagcagtt gtattaaaat tactcctgtc ttgtactgat 71460 aaagaattag cactagcttg cttcgacaag atcaagcccc atttcttctc tagttcctac 71520 ggtacaattc ataaggctat cgctaaatat tacgatgaac atggtaaaat accaagcatg 71580 gatgagctta gtatcaaatt tagtcgtaat aacaacctac aaatggcact agcagcctta 71640 aagaacctag aagaattaga catagacttc gatatggcag tcgatgctct tagaaatgaa 71700 tacgcccaac aagaggcgtt gaccatgatc agccgagatt tattagacga tattacaatg 71760 ctaaccgcag aggacttagt tgatagactc agtgctctac cactgaagtt agaggaaaag 71820 attgagcata gtgggagtat tcttactgcc tctcaaatcc cagtattcca aacagaagag 71880 caatcacagc tagacttgat gtacactggt atttccaacc gatgggactc agagtttggt 71940 ggtgtaggta gacaagagtt aatcttctta ggcggtaaaa ttggttctgg taaatcggta 72000 ttttgttcta acttatgtgt tcaacagtat tatgcaggga atattgctcc gtactatact 72060 attgagatga caggtagaga aaccttacta cgtatgttgg ctattatggc aggggtacct 72120 gctatgaaag ttaagcaagg aacgcttgaa ggtgaagaac tactaaaact agcgttaact 72180 agagcaaaaa tgtttcatgg tggtgcagaa gcctataaga attttgtacg aaataaaaac 72240 catcttaaag cctcagactt ttttgagcta gatcagcagt tgatgcgtaa tcatgaagaa 72300 acgcatccat tgattattgt tgatgattca gaccttagaa tgtctactat tgatgttagt 72360 ttaagtaaat tcaaatccca atacggcaac aagcttacta tgggtgttgt ggactacatt 72420 aacgaagtaa gactagatgg gcaaggtgat ccttatgatt gggtttacca gttagaatta 72480 tcacgaggtc tgaaacgcct agcacgtaaa catgactgcg ctatattttc accttatcag 72540 ctaaatgata atggtgaagc cagattctcc aaatctatac tagttccggt ggatatcgcc 72600 atgactctag atgcagacca tgaagcaggt ataattcaac taaacgccaa gaaagtacgt 72660 tctctgccac cttgccaatt taatgtaggt attgactgga atacgctgaa gattgaccct 72720 aaagaggtat ctttagaaga agtggaacaa agtaaggcag aggcacaggt ttcaacatca 72780 gtacaggacg ctagttatga actcgactaa tgtggaaaat ttattaaaag acagagatat 72840 acactacgta gacaagggga gagatctcct tgtcaagtgt attaatccag aacacgatga 72900 ttccaatcct tccatgcgga ttgacagtga aacagggaag tttaactgtt ttagttgtgg 72960 ttatcacgga aatatatttg tccactttgg ggagtatcaa agccctgttt atgacttagt 73020 ttacagcgtt aaagataaaa tcgctgagat acaaagagaa actcgtggga tggaaatccc 73080 tgctggcgcc cgtgactatt ttgacgattt ccgcgatatt tccgcagaga cgtacaaaaa 73140 gtttggtgca tttacttatg ggcatgagga ttatgaaaac cgtattatct tcccaatata 73200 cgatgccact ggtaagatac aatgctttaa cggtcgccac atgtactcgg acgtaaagcc 73260 gaagtataaa atatacccag aaagagcagt tatacctata ttccctttta taaaaggttc 73320 tgacaccttg ataattacag aggggctttt tgacatgctt aatcttcatg ataaaggtat 73380 agaaaactcc gtgtgtattt ttggtacaca taacttaacc ttctcaaacg cataccataa 73440 actattacct tatatgattt caggagtacg tagattctta atacttctag ataatgataa 73500 ggcaggtagg tacgcagcta agaagatga agatattata cgtatgaaaa ctaagatgga 73560 agccttagat attagttaca tgttagaacc tggcaaagac ccgggctgtc tcacacaaca 73620 ggaagtggac aaactagcat ttcaaattga aaaactaatt gcaaggtaat cctgattttg 73680 atataatact gaggtaattc ggagatttct ggattacctc tttttatttt ggttgttaca 73740 tcccatagca attgtaagga attatatgaa agtagctatt attgataagt gccctaacaa 73800 ggtaaattac gaaaaacttt tcggatttcc agtagaagtt ctacacctag ctgataagaa 73860 agttcagcgt ctaaagaaag ctgatattac tctagagcta aacccgcatg actacgactg 73920 gattattctt gttggctcag aagctctaaa acaatataca cgtcatactc gtgtaacaga 73980 ttactcaggt aaacaagtag aatctaaaga tggttcatac aagaacttct tagtatcctc 74040 taaccctgcg atgttgttta ttaagccaga aactaaacct gcgtttgacg caactgtagc 74100 tgagatccgt tctatcatgg acggtacaaa agaagctaaa gcagaagtag attatgcccc 74160 tattcaaagt acagaaggta tcctagagta tctaaatctt gtgtatatgt tacctgttgc 74220 gcaagtacct gttatcgcac tcgatacgga aactacaggt ctagcagcac gtaaatgtca 74280 acctctaggt atttcaatgt cccatagaac acatcaaggt gtgtacatgg acgcagatta 74340 ctttgatgat gaatgtgcgg agctattaca aaaaattatt gacacgccga agcgtaacgt 74400 tgttttacac aactctaagt tcgatgatca catcatgact taccacttcg gtactgattt 74460 cgaaaaagca caccaagaag gtcgtctaca cgatactatg gttatgcact atgtacttga 74520 tgaacgtcaa ggtacacacg gcttgaagtc tctagcgatg aagtacaccg atatgggtga 74580 ctatgacttt gcactggacg agttcaagaa gaactactgt aaagagcata aaatcaagca 74640 ggaagagttc acttatgacc ttattccgtt tgacattatg tggcaatacg ctgcgggcga 74700 tactgacgca actattcgtc tttaccataa gttctggaac atcctatgtc gcgatacctc 74760 attcaaactt aaatggttgt atgagaatct actaatgcct gctacccgtt tcttgggtcg 74820 tatggaagaa cgtggtattc cactatcacg taaacgattg tatcgtgcac atgagcatct 74880 agaactacaa ctacgtcagc tacaagaaaa actatatgag ttcccagaag ttaaagagtg 74940 tgaagcacta cttgacaaac agttcaatcc gaactctact caacagctac gtactctact 75000 gtttgatgta gtaggtctat ctccaacagg taaaatgaca gactcaggtg ctgactctac 75060 cgatgcggaa gtattggaga aattagggga acaacaccca atccctaaac ttatcttgga 75120 tattcgtaag actagtaaat tgattaactc atttattatc aagttactag ataatatcga 75180 cgccgatgga cgtattcgta caaacttctt atgtacaagt acaacttctg gacgtttgag 75240 ctcatcgggt acctttaacg cgcagcaatt acctcgtgat aacccaatta tcaaaggttg 75300 tattgtggct ccagcaggtt acaaggttgt agcgaaagac ttgactaccg ctgaggtata 75360 ctacgccgct gttctatctg gcgaccgtaa cctacagcaa gtattcatta acatgactaa 75420 cgagcctgat aagtacgcgg atttccactc aactattgct cacatggtat ttaatctacc 75480 ttgtaagcct aatgaagtta agaagaagtt ccctgcaatg cgtcaagcag ccaaagctat 75540 cacgttcggt atcatgtatg gtagtggccc ggctaaagtt gcagaatctg ttaacattgc 75600 actgctagag caatcacttg agacaggcga accatttgta ccttgtacta ttgaagatgc 75660 gaaaggacac attgatactt acttccgtaa gttcccacaa ttgcgtcgtt ggattaactc 75720 ttcacatgag caaatccgtc agtatggctt catttacaac ttctacggtc gtaaacgtcg 75780 cctacgtaac ttcaagtcta cagaccgtgg tgttgtagcg ggtgaagtac gttcaggatt 75840 taacgctatt atccagtctg catcttctga cagcctacta gtaggtgtta tggaggccga 75900 taaagagatt atggctaaag gtatggacgc tgaaattatc ggtctagtac acgactcgat 75960 tattgcaatc gttcgcgaag atttagttga tgaatacaat gaaatcattg accgtaacgt 76020 acaggattta cgttataatg gttgggatga tgagccgttg acaattaaag gttgtccaat 76080 cggtatcgac tctgactcag aagatggtgg ttctcgcgac tactcttgtg gtaagatgga 76140 taaagcatac ccatttattt ctgtgtacga tagtccagaa atgcaggcta aagcagaaga 76200 agtctttgag aaaatcaagg aagggtggac tcctgaaaaa gacgataaag gtaagtacgt 76260 caatcctaaa gagggcgcta tctatgacta cagggagtgg gttctagagt cgctacagga 76320 tattggcatt gcagcctaag ttcaaagcca taactccgtt ccaagctaaa tttcctctgt 76380 acgccctaag agcgtacgag gatattaaac agtttgagga gtttatacta ataacttcca 76440 aatataggga atacatactg gactgtcctg cattggaagg aaactatgca gaacgtagag 76500 taaaactgct agggatggca gaccaattgg aattcaagat atatccattg agagaaagat 76560 tcacctctct aagccaacta gctaactcta aaagaagatg gtttatagat gcggagggct 76620 gtattgttag atataaacct agcaagtttt atcatataaa atatgttaaa gtattaaggg 76680 ctgacagaac atggaatgga tactacaggc taatgacaaa acttcctgta gcatttgtaa 76740 cagagcaggt ggcagaatat gttggttata ttcaagtagg tagtgcattc tatctctatg 76800 aagtaaccaa tgagaagaaa gccatatcta ggaagaaact ttgaaagttg tattgtcaaa 76860 taaagcgtat ttgaagcctg atgatcaatt aagagaaaga ctagagaaga acttgactta 76920 tcaaatcttc gagccacact ctaagacttc aatacctaaa ctaatcttac actatgggct 76980 agtagctaaa ggaacatact ggcttccagt gtcgcgctta gatttgcttc aaggatatga 77040 agtagaatat attgataagc gtaccatttt accagagact tttcctgaac ctaagttcac 77100 tttacgtgaa gaccagcaag aaatatttga tgaagctgac gattcttgta ttataaacgc 77160 aaagccaggt ttcggtaaaa ccattctagc attagcatta gcttggaagc taggacagaa 77220 aacacttgta gtttgtacca ctactgcaat ccgtgatatg tggattgacg aagtacgtaa 77280 gtggtttgat atagagccag gtattatagg ttcagggcag tttgatcatg agtccccaat 77340 tactattggt aacattcaaa cactatctaa acacggtatt aaactagcag accaatttgg 77400 tcttatgatt gtagatgaga tgcaccatac tccggcatct acgtttacta agcttcttat 77460 ggaaagtaaa gctagatata agattggtct atcgggaaca ttaaagcgta aggacggctt 77520 ggaatgttta tttaaagact tcttcgggat gaaggtattc gtacctgagg tagcaaatac 77580 tatcccacca accattcact tatacgatac tgatgttgag ctttctggta atcaaatgat 77640 accttgggct aacaaagtaa atgctgtaat ggaaaaccct cattatagaa agcaggtgct 77700 agcacttgca aattgttata taaagatggg tcataaagtt atcgtcgtga gtgatagaat 77760 tgaatttttg aaatatatac atgaacatgt gaaaggacgc tcggcattat tcattggaga 77820 gacctctact gaagaccgta ttcagctaca gaaagatatg tcagccggaa aactagacat 77880 atttaatgct agtcagaata tattctcgga aggcatttca cagaatgaac tatcctgtat 77940 gattctaggt agccctattg gagataatga gtctctatta gaacagctag caggtcgtat 78000 aatgagaaaa gcagaaggta agctagaccc tgttctagta gatttgaagc tagaagggtg 78060 gactggtgga agacaccgtc gtgctcgtac tgctatttat ggtaagaacg gttggcaatg 78120 tatcccaatg tcaatcccta agctagtagc tacaaataaa aattcacttg ctaagttgct 78180 agattttaaa gtataatata tgcatattct gaagaaagag agaagattat gaaaacacta 78240 aaagtagatt tgggtgggaa acttccagat aatgtgttat ctgtttgtat atacttagac 78300 caccttgcaa tagtaaatgg cgattcaatt gaagtaggta acgatgaact gctgtaccat 78360 acactagaag tcaaaccact tcacggagag caagaaactt ccgacgaacc ttggaagcct 78420 ttcgacgctc cgcgaaaagt cacggtagat tttccagtac ttatgctgga taaaggtgca 78480 cgagcgtatg ctcttgtagt tcaaccagaa aaagaccttg aagctatttt agtagtagcc 78540 gtaggcaatg aagaacgttg gtatgacttg ccaaaagatt taaaaattga attgctcaac 78600 tactaaaatt tctgtataat aatcgcataa agttgagaaa tggttcttta cgactttaat 78660 aaaatctggc tgctctcagg tgggcagccc agactgatgt taagatattt taaatatcta 78720 tacctcaacc gagttgatta ccgtttccta aaaggaacaa acttcatact caaccctgaa 78780 atcgtagtta ataaccctat gagactgcca caacataagt tggcagagta tctaggttta 78840 tgtgctttac gcaactacgc atcctatcaa caataccgcg aggttaatct agatttggaa 78900 tatttccctg actatatccc tcggcaggtt gtcgatgaaa acccactaat cgcattaaac 78960 aaaacaaagc taatattcaa atacgaggaa aataatttat gactatgact gcatctaact 79020 ggggtactgt aacagcgggc gacaacggcg gcgagaaaaa atacactaag tacaaagctg 79080 gcgctaacaa attccgtatc gtaggtgaca ttctaccacg attcgtttac tggttgtcaa 79140 ctactaagac tgatgacgaa ggtaagccac gtaaactaag tgctcctttc gattgcctat 79200 ctttcgatgc tactccaggt gttgaaaagt tcattaacgg tgctcttgac cctatcaaag 79260 aactaggtat tcaaaacact gactggcaag gtaatcctga gtttgacaaa aaaggtcaac 79320 cagtcccact taaaccacaa cgccaatacc tattcccaat catcaaccgt gattctggtg 79380 aatatgagta cgctactctt aaaggcgaca ctcttgcagg tatcggtgag ctaatggcta 79440 aacttagcga ccctaaacaa cgtcgtcgtt ttgctgaccc agactatgct gttaacagcc 79500 caatggacat tgatgttgtc ctagttaaat caggctctgg tttaggtact aagtacaaaa 79560 ttgacattgt tgaaacaatg gaaaacgtac tagacgaaga tgcgttcaaa gctatgatgg 79620 ctaacttcga agcggatgct gaaatcttga aagataagaa gccaatccaa gaagtgttcc 79680 cacgcccaac atatgcagaa caaaaagaag aagttcacaa gttcctacac ggtgacgaag 79740 atgaagcagg tgaagctcct gctaacgaag ctgctcaaga agcaatggat gaactagact 79800 aataacaatt ggggaggctt tggcttcccc ttttacttgg agaaaataat gacagaacaa 79860 cgtaaaatca tctttgataa acgctcactt acaggtgctc ttgttcaagc agcaggtgga 79920 tttctagaca atcccgtaga gaataagact tggaaagaag ttgaagatgt tcgccgtgag 79980 attgcgatta tccgtgaacg agttaacgag tatcttacag aagtagatac ttactttatg 80040 aacaactgta aaaattggca agagcaatgg gacttggctc atgagctatt tgaaccattc 80100 aacgaacctt ataatcctga tgagattgat attgagaccc taagtaaaga gttccaagaa 80160 aaaggcttgg tgaaggagct agctgatcaa gtagctacta ccgtcaagta tccaatcgag 80220 aattaatggc tagactttta ttttcggctg attggcatat taagttgggg caaaaacatg 80280 tcccaactct ctggcaaatc aaccgattcc actctctggt agatttactt aatagaagat 80340 tcgaagctag cgagtgcgat attcatgtta tcggtgggga tatttttgac agattcgacc 80400 ctacccctga agaaatagag ctttattttg acttagttgc tagattaaac cataagacat 80460 tgatttacac tggcaaccat gaaatgaagt ctaaaacgaa atctgtccta gacaatcttg 80520 cggaagagac aaatcgctgt aactctcttg tcgaaattgt cacagatata tatagaagta 80580 aagattttga tattgttgat tataaagagc ttcacaaaaa atcttggaaa gagcaacact 80640 ctcgtctttg cttaacacat gttcgcggtg aaatcccccc acatgtaaaa cctgaaattg 80700 acttgtctaa gtttgattgc tatgacttag ttatcgcagg tgacttacac tcttatcaaa 80760 atactcaatc gactgaaaac ggtactacaa tcctttaccc aggcagtccg cttactacat 80820 cgttccacag agaacgtact aataaaacta atggatgtat cgttatcaat accgagacgc 80880 tcaactatac ttggtatgac tttaaacagc ttccccaatt aattcgtaag actattcaag 80940 tcggagaaga aatgattaag gatgagcatg atagagttat ctatgaagtt gaaggtgata 81000 tttcagagct taaaacaatt aaagattctg agctattaga taagaaagtc aataagaatg 81060 ttggcaaacc cgcagcacta gacctcacta ataaatctgg aacagatgaa gaattggctc 81120 tgtatcttac agaggtggag aacctgccta aagatacagt tagtcgttta gtaggtaaat 81180 ataaacaggt agttcctaat gcagattaga ttacatcgag tacgtggtaa caatgtacta 81240 gcatacggtg agtttgattt cattcttgac gaacatacgg tatatcaatt aattggtaag 81300 aatggctcag gtaagtcatc cttaccagtt gttctagagg aaatcttata taacaataac 81360 tctagaggta ttaagaaagc atcgttacca aatcgttaca ccgacgtcaa gggttggtgg 81420 ggagaagtta ctttcttcat aggtgatgat gagtatgttg tccacaaaga agttaaatca 81480 gcagccaaag taaaactata taagaatagt gaagatataa gtggtcatac agctacacaa 81540 acatacaagc agataaaaga aatacttggg gataccgagt ttaaaacatt caccaagctg 81600 gtataccagt cgatggtatc atctatggac ttcctaacgt ctacagatgc taaccgtaag 81660 aagttcctcg tttcacttct aggcttggag ggctacggtg aaatcgaagc tgctctaaaa 81720 gaagctagaa agaatgccac ttctgtctta aacactgtta aaggtaagac ggagacaatt 81780 gagaaatggc taaaacgaaa ctcaatcata ccagacgaaa tgtcgctagt agacgttcca 81840 gaactagact ctggtttaga agagaaactc aacgagaaaa acgtagattt gcgtaccgct 81900 gagattcaca atagagaaat cgacgctcaa caaagacagc ttgacgattt ccatacaaaa 81960 gcccaacaca aagataagct acatgccaaa gtgttggagc tacagaaaaa tgaacctgcg 82020 ccagcagagg acttttctgg aaaaattcaa agtgttacgc gtgacctagc aaataagcaa 82080 gcagagatgc gagtagagaa aactacttac gagaaattca aaaacgaagc tagcatgact 82140 aagtgccata cttgtggttc tgaactagat gtaacacaga aagcagaagc tcgtgatgct 82200 gctaagaaac gattctataa tcttaaacct ttacgtgatg cacttcaaga agagctcgat 82260 aagcttgaag ataaacaagc attatataaa gagtatcaag attggaaacg caagatggac 82320 aatgctctac tagagtttaa tacctatcaa gtgcctgagt acccaggctc tgtagacaag 82380 cgcaaatccg acactaattt actacaggcg gaaattcgag agctacgtga taatataaac 82440 aagctcaaaa ccgacataga aaatgctaaa cgacataata cagatgcctt agtaaataat 82500 gcaaaacgtg aggagatttt aaagacctct tcggaatatc gcgttgagtt ggagaaatat 82560 caggttgacc tacaagaagc agagcttgaa gttgcggatt tagacattct ttgtaaagca 82620 ttcggttcta aaggacttat tagttataaa atcgaaagca gcgttaaagt ttttgaagaa 82680 ttaataaata aatatctttc aaaatttact gctggtcaat tcgcactagg tttcgaacta 82740 gactctacta aactcaaggt tgttatctat gatgatggtg ttgaagttgg gatggaatct 82800 ctatctagcg gtgaacaaag taaagtaaac gtatcaactc tactagcgat tcgaaatctt 82860 atgagtgcta tcagtgacgt taatattaat cttctattct tagatgaagt gatttcggta 82920 ctagatgatg atagtcgaga tttattggta gagcttctac tagaagaaac acatttaaac 82980 acatttcttg ttagtcatgg gtataatcat ccactaacaa aagatattcg cttggagaag 83040 cggaataaag taagtacggt agtaaatggc tgattcaaga gataaaggta agcgcggaga 83100 atatcaaatc cgagatttac ttagagaaaa aacaggaatg gagtgggaac gtgtcccagg 83160 ttcgggaggc ttcggtgcct cccacggatt gaagggagac atctacctac ctcacgcaac 83220 tggacatatg tccaaatttg ctattgaagt taagtggtat aaagatgaac acttaaactc 83280 aaatataatg aagactacta gcactcaatt agataagtgg ctagaccaga cctatcgtga 83340 ggcaggtcaa atgaacgcta agcctatgct agtattcaaa aaagacagag gtgattggat 83400 tgtgtgtatt gatgcatccg attaccaaga attatcgggt aaattagcaa gtgcaaaacc 83460 gatgataaat tataataaag gtgatagatc agttttcatg attaacttta aacagttcct 83520 atcgctgatt gaaacgggag atatagtacg atgatggaca tgtggaatat ggcaacagaa 83580 gaagcaatga tgcccaagaa taatcttctg atagtcgatg gattgaactt agcattccga 83640 tacaaacatc gcggtactac tgactttgct gctgattatg tgaaaacagt aaattctcta 83700 gctaaatctt atcatgctcg tgagattgtt attctagtag actacaaggg ctcatggttc 83760 agaaaagact tacatcctaa atataaattc gaccgtaaag ctaagttcgc tgaccagaca 83820 gaagaagaga agctagcagc agagcagttc tttgaggact ttaataaagc tattgagctt 83880 tgtgagtgta acttccatgt tataaaacta gaaggtgtag aggcggatga tactgctgca 83940 tacttagtgg aagagtttga agatacagat gactttgacc atatctggtt aatctctacc 84000 gataaagact gggatgaact tcttggagag aaagtaagtc gtttctctta cactactaga 84060 aaagaatata atttagataa cttctacgaa tatcatcagt gtgatacccc tgatgaatac 84120 acatctatta aagctatcat gggcgaccca ggtgactctg tatacggtgt taacggtatt 84180 ggtgcaaaac gtgcttacaa cttagttcgt cagtatggcg atgcgctaga aatcgctaca 84240 caacttccga ttgaaggtaa gcagaagtac atcgaagaat taaataaatc tgaagaaaag 84300 ttgattttaa atacccaatt ggttgattta cgttctttcc acaaggaagc aattgctttt 84360 ccaagtccag agaacctact atatctagaa caagtttgca ataatttacg aggagcagca 84420 taatggaaaa aatccgcatt aaactattaa acgaaaaagc agaaccacgt tgtatgaaag 84480 atggagacgc aggtatcgat ttacggatga acatcgaaac agcacaggga tttactccac 84540 tattacgtgg tgaatcaatc actttcggta caggcgttaa aatggcaatc cctaaaggtt 84600 gggtaggtct tattatgcca cgttcgggtc ttggattcaa atatgagatc cgattggcaa 84660 atactactgg agttattgat tctaactatc gtggtgaaat catggttaag atgcgcaact 84720 gcggtgatga ggatgtattc ttagaagact ttgagcgtgt atgtcaaatg gttattgtac 84780 ctcattacgt agtatataat aatctagaat ttgttgatga actagatgaa acaaatcgtg 84840 gcgaatcagg attcggagaa tctggtcgtc aataatagta aagggagcca attggctccc 84900 tttagtcgtt tatattaact gtcatttcaa tagtacgtcc tctattatct cgtaagaaat 84960 taaacagaat tcgtttatct tttggtgtaa tcctaacgtt acctgtggcg gagtatctta 85020 caaagaaagc attagagctg ttaaaccaag cattagtagt tccgacacct tttagaataa 85080 taccagtttt accttgggtt aatgtattat ataatcgtgt actttccgca acactatttt 85140 ttacctgtat atcaatccaa gcatccctag ggctagaagc cctatcctct tgccatgcaa 85200 agaatttaat agctgtacaa ggataatcac taggatccat atcaaactta gtaatatcta 85260 ctgtaccgta cctactagct cccctataag aagtatccac tatctgctgt ctaaatacta 85320 ttttagtgaa agcatatgcc attttacgcc atacaccatt gactttagtc caagtggcga 85380 ctccttttcg ccacttgcca cttactttaa cccatgcaac tccatctttc caggtgccgt 85440 ttagtttaat cttcataaag tctccttact tgtattgaat gtaaatatcg ccgtcagacc 85500 cacctgtagg attaccagta ccataagtaa tcttacgttt tctatccgca gctagtttag 85560 gctccgcccc tatatcttta gcagaaggtc tgaatccttc atggtagcat ttatatcttt 85620 tagagctaac atatacctga ccagctgacc acagcccgtg ttcacccata gcggccttag 85680 gtaccccgtt tatacaccaa accccttggt gtcctccagc catagtaccg cctgtaggat 85740 tgttagtatg tttatatcct aaaccataca aagtaccaaa gttattacca ctagcatcgt 85800 ttctccagct agtacccata caccagattt gatccgtttt tctagagtca taggtaccaa 85860 atagccccct atttctagca gaagaaatta aatctccatt aaaagtacca ccgtttttat 85920 ccatgaaacg actattactc tctgatttgg agtacacccc taactcacct ggagtaggtt 85980 ttctagcaga gttgtattgt cgtctccatc ccggagagta tccgtctcca ttgaatacat 86040 agataaattc agcattagta gtacctccgc cagatgaagt tgtaggagtt gtaactcgta 86100 tagtatagtt actttctcta cctattactt caatcacaca gccagcaaaa catatcttac 86160 catgtccagt atccaatatt ctattactag cgtaagccca gctacatcta catacccatg 86220 acatagtttt aaaggcgcct tgggatttta gccaggaaac catctgtgct gttgtccagg 86280 tacccccgcc tgtacttaaa ttactactgt atgccctgct aaaccccgta gagtctattc 86340 cgtccaatgt agtagcgtca ggtttagcat ttttactcat gtaacgagta tcagcagtag 86400 tctgtgttaa taagttacct ggtttaccag ctacctcact ccatttcggc cagcgtgtag 86460 cataactagg gatacctgtt atttgagacc atggatgtgt atgtgtatca ggtggcatag 86520 taccaggttt atcactaatt tcaccccatt ttggccaacg tgtagcttgt gctggcttgc 86580 tagatacatc attccatgaa ggtttccatg agtttgggtg tgcccctact tctgctgcag 86640 taggcttctg ggcagtagta tatacttttt cagaagtagt ccactctcca tcttgctcac 86700 cctgtacgtg gaataatgca ttagagtttt tacatgctac aataccattt actctacccc 86760 cagtagcatc actatatgta tccataacta ataagtctga gtactttccc ataggtctat 86820 tagttgcagg aaaagcggca ttatctacaa agaaaggtcg tactactctt ttctttctac 86880 cagtatgagc agtctcagga gtaacatctc gtctgtcagt agcttctaaa tatagtgctt 86940 gacctgcagt atctgctttt gcagagttag ctgccttacc agtggctggg agtgccccaa 87000 tatctgaggc acttggttta ttattgggag agtatactct ctccccttgt tctgttatag 87060 ttgtttctgc ttgtaaatta tcaacgcgca ttataactcc ttacattatt ttcacgagct 87120 taacagtaaa gtctaccgca gcagagtatg ctcggttaca tttgatttca acggtatgat 87180 cacctttgtt acctggctta gcatgagttc ttagataaat atactcacca ctgtctgcat 87240 gtcccatatg gtgtagcgga atctcagaga cgttatcttg atttgtattc cctgcgtgcc 87300 agtagaattg accagtatag ccctgagtgt aagcaacccc gcctttacct gctgaagtat 87360 aagtaattaa tacctgatac acaccagact ctttaaggtt tgatttagtt agcagagtag 87420 cccaaacatt ggcttggagt tttaaactct tagtaactgt atcaggttgt gctaggtctg 87480 caatcttagg cttgttgttg ttagagtata ctctatagcc attttctttc agagtaccac 87540 ttttaaggtc taaaccagta tctgagaatc tagctaaagt ctctatgcct tttctaatat 87600 caacgtaatc tctggtagtc cctagaagca ggttagtact agaaacaact tcattaccgt 87660 caatacccat tgtatcttct actactaatg aggcattctg tggcctactg ccacctatag 87720 tagaaccggc cttcatctca actttaccaa tagtagcagt actagtacta gccttagtat 87780 ctaactgaga ttgaacccaa tctctgcgag ttaatgagtc cccataacta gattgcgcag 87840 cactaactct aggactacca ttaaagataa cttgatcaga accgcgagga accctcatgt 87900 aggtatattc cttaccacta tttactgtac caaaccaagt attgttatca gtagcacctt 87960 ggtaacgtat gtatgtaccg tgctgcttgt tatcttcggt tatatagaaa gtaccatttc 88020 gattagtacc tacatctaac gctaatatac cttcgcctga tttaccaact cgtaagtctt 88080 gacctttacg gaaaaagcga gcatccgcac ttgctttaga gtaagcatag tcagtatttg 88140 ctagtccatt acgggcatct gcaattacct gagctagagt cttactttca aggtggttgg 88200 cattgttagc cgtaccattt tcaggtaagg ccccaatatc tgctggggtt ggcttattac 88260 cagtatggta aatgtcctga cctttataac gaatagattg attactaaag taaaataact 88320 ctgtaagcct atcgttattt acatctttct gacctcctcg gaaactaaag ccaccgtata 88380 cagtatccgc ctctgaagaa tcttcaaagt tatcgaagta agtagtagta tcttctcgcc 88440 taatgttcat gtactcaaca gcagggttag tgtatctaga tacttttaat ccattaacac 88500 cgttgttaga tactacatga acgtctccat ttagttttgt attaccaact atatctaacc 88560 ctttagcaaa catcttagct atataagaag ttgcaaacca ccaaccgctt gtacctaacg 88620 tagatttacc agtagtattt ggggatgcag gcaagaaccc tgtatcatta tctgaagcac 88680 gtaaccattt attagaccct acttgaagat gagtatcgac agctttaata tagctattac 88740 cacctacttc agcccacgta ggccaacgtg tagccattgc tggcttacca gaaacattgt 88800 cccagtcggt agggtactta ctaggctttc cagtaacctc attgaagcta ggccatcttg 88860 ttgctgctgt tggaatatct ttcaatttat tccacggcat tctatttatt ggactgtatg 88920 ctgatgttaa atccagccac gggttacaat cattatctac ttcttctata ataacagata 88980 ttctatatgt cttatcatgc cctgttcgta gtttaggtac tcctttatct aaaaatactt 89040 ctggatggtt actaccagac ccctgtcgag ctactaaggt taggttagtt gtacctgtac 89100 taccccctac ttggttaaaa cctttaaggt agtatactgc tccagtaaat gtaccagtgc 89160 ctactgtgta tgctcgaata taataggact tgtcagccat ttctagaggc ttgtcctcta 89220 tattaagaag aacggcgtga atatctttat tgacgttgta tatcttacta tacttcttcc 89280 atccatctcc ctgagctact gcacctaaat cataaggggt aggtttattc ttctcagtat 89340 aaatagtagc atcattatgc gttaacttag tacctagtat attaacagta tctaaactat 89400 cgttacctac attaacttta tctcctgcgc tttttaatac ttctttatta ctagtagtta 89460 gtcctcttaa cttaaaagta cccgccgtaa cgttacctgt tacttctacc cctcctgaaa 89520 ccgttccccc tgctttactc aggaaggtat cttgtgccca gtctctacgt ataagtgaat 89580 tgccagcact agattgtgcc acacttactc ttggtatacc tataaagata gggaaatttt 89640 gccccctacg agcaatcatg aagggtgctt tattaccctt atttattgta ccatagtggg 89700 tttcattggt gtccttgcct tgataggcta tataggtacc atacccgttt gcttctgtca 89760 gtgatagttc tgcattctta ttagtacccc cattaacact gaatgcagca tcacccccat 89820 gagtaatttc cacgttacca gtaaatactt cgccggtttt tctagcagca ccaatacttt 89880 gaggggttac ggtaacagct ccagtctcac cgtttacaga agtaacacca gtaccagaat 89940 caataatatc gaatttcttc tcatctagaa tgtatactag gcggtcaccc cagttccatg 90000 ttttaccatc aaatactttc tctaattgtc cggcatttaa ctgaacatcc caacgtgagt 90060 tagtaactgg ggttttagga tagctaccag agttagggtt ccatgtacct ctatatacac 90120 tacctttacg aagttctatt gcaaaacttt cagcttcatc acgagacttt ttggctgcta 90180 cttctgctgt tttggcctct gaagcagaac tagctgtgtc atcagcacta tttttggccg 90240 ctgaagctga attagctgct gaagaaacac tagcagctgc ttttgtttcg ctagacttag 90300 ctgcactttg ggaggttttt gctgctgatg cacttgccgc tgcttttgtc tcactagact 90360 tagctgcatt tttcgaggct atagctgtat cacgagcctt ttcagcctca agttgagctg 90420 caagcgcgtc actagcatgt gccatagctt tagtgttagc gtcggctgcg ttagacgcgc 90480 ttgtagctgc gctgtcttca ctagactttg ctttggtttc actagactta gcattggttt 90540 cactaacttt agctgcattc ttagactgag ctgcttgatt tgcactagta gctgccgctt 90600 tagccgaatt agaggacgca gctgcacgac ttgtagctgt agtagctgct gtactagctt 90660 ttgtttcact agacttagca ttagtttcac taactttagc tgcatctcta gcagcttccg 90720 ctgctgcttg agctacttct gcattagtct gtgcggtttg tgatgcttga ttagagtttt 90780 cagattcttg tgctgattgc tctgacgcac tagcagatgc cgcagctgct tgcttactag 90840 cagctgcttg acttgccgat ttacttgcag ctacctgaga tgcttgggaa gcttttgctg 90900 aggtatcaga tgctttagcg ctattagctg cagctgtctg tgaacttttc gcgttggtct 90960 cactagtctt agcgttggtc tcactagttt ttgctttggc ttcactattc ttagcattag 91020 tttcactaat ctttgcagca tcctgagaag cttttgccgc atcctgagat gctttagctg 91080 cgtccgctga agtacttgct gccgctgctt tctgagaagc cacaactgac ttagctgtag 91140 atacatctct agcattttca gaggctagtt gtgccgcttc tgctgctatc ttggcagcgt 91200 ttgcatctgt tactgattga gtcattgaag atgctgaagc tgctgcttct gctgatgact 91260 tagctgcatc tgcttgatat tggtttgcct tatcttcact agcttttgct aattgttgtg 91320 atctataagc attaccagca gattgagaag catctcctgc actagagcct gcttgatctc 91380 tagctttttc tgctgctttt tgtgctgcca aagctgcttt ttgttcagca tctgctttag 91440 ccgctgaagc tgttgctgat ttagcttgat cggtagcaat agttgcttga gccgtagctg 91500 ctgttgcttg cttagctgct tctactgcct gagcttttgc ttctgtttca cttgccttag 91560 ctgctgtttg tgaagctttt gccgcatcct gggatctttt agcggctgct tgagagctct 91620 tggcggatga tgctgaacct gctgcgtttg tttcactagt ttgggcatcg tgtgcacggt 91680 tatctgctgt agctgctgct tgttttgcgg tatctactaa gtttgtgatt tgaccgctaa 91740 tagcgtctaa gtttctaata gtttcattag ctttatctat ctgtctttga gactcggcta 91800 ctgaggactc cgctttactt acagacttat ctatttcact ttttacttct actaggtcgt 91860 caatacctac aacttccatg ctttcggaaa gttctacatt tatctcagaa ccactaccat 91920 ctagtaagct gttctcaagt gatactgtac ctgtaaactc ttttgtatct atttcggcat 91980 tgacagggcc tactaaatcc ccgtcaagta ctctttcttc tatctttata ttatcggcca 92040 ttattattcc tctatgagat attgcttaca acaactaact gataagaatc tgcccccagt 92100 actccactag gcacagcttt tacttgtttt gagttaccat accatgcaac ttctgccgta 92160 ggtactgtag ctgctaatac acccactggt tttgtttgga aactatttga tgtacttggc 92220 atcttgaaga tatttactac cccatcagta ggagtggttt gaataccccc actaaaatag 92280 cgtataccag caaagtagtg tggtttagtt ttatctacta caatacttac aggttgatca 92340 attggagtaa ctgctgattt agttactgta cttcctaaac cactctcagt tagtgggtct 92400 aatgtagcag ctctgcttaa ataacattta ccttgataca ctcgtgtagc tgaccccgta 92460 ggacttatca gtaacacat gtagtaacca agctcataaa ccctatgaga atctacttct 92520 aaataacgtc tatctgtaaa cttacctagt gctgctgttt gttctttagt tagaccaagg 92580 taggctaacc cgtctttacc agaagctatc gttgcctcaa agtttactat taacttagag 92640 tcttttgaga aagatgttct aagctgagct acgatagtgt atccagtaag gtctatcgga 92700 gctttttgtt ttataggaac ttgtttgtta gtaagcgggt tcagttgaga acccgttact 92760 tcctgcttag taaactttag tacgtgagca aaagtagctc cttcttctat ttttaaatta 92820 actatcttac tcataattat cttatatgta tagacccgcg agacaagtct atcttcattt 92880 tataacgaga ttcagggttt gatgattgtg ctgctgcatt atcatatata acattaacac 92940 ctatttgttg taaaaaggca ttgctagcaa tgattttctg agcagtgata gaaccattaa 93000 caatcatatt accatgtact aacatagcgg cactttgcca actaccttta atccattgac 93060 gagtctctgc cgaggcagga ttgctaggat taaattgagt tagtacatcg tactctactg 93120 ggcctttacc aaaagtatca gtaaagaact tagttgcaga cgtagtatta aatctagaga 93180 aacgacttat tggctgagca tagaagcctg ggcccctact accatcgcta cccggcctac 93240 catctctacc cggtctacca tctttaccat ctctacctgg acgaccatct tgtccatctt 93300 caccatcttg tccgtctcta ccgggacgtc catcatcacc tgcaaactta gaccacacgt 93360 agtcttgagg tctactactt tctgtaggtg tggttttatt atatgcaaaa cctatatact 93420 tcttaccgct agggctatta gacataccac taccactagc attatcggca tatttaatcc 93480 aagtataata tgttttaccg tctttacctg gcttgccagg gacaccttga tcaccctcta 93540 acttactcca agaatagtca gtaggattac tgctttcagt aggtgttgtt ttattaacag 93600 caatacctat atacttctta cctgcagaag tattagacat tccaccaccg gtagcagtat 93660 cagcgtatcg aatccaagtg taagtagtta caccgtcttt accaggagca ccaggaatac 93720 catcacgacc aggatctccc ttatcccctt tgtcaccttt aatgcgactc cacgtgtagt 93780 ccgcagggtt actactctct acagcagaag tcttattata tgcgaatccg atatactctt 93840 tcccgctagg gctattagag ataccgcttc cgtctgcact atcagcatac ttaatccaag 93900 tgtagtaagt aactccatct gccccaggac gtcctggaac cccttgatca ccttcaaact 93960 tactccatgt atacacgtta gggttagtac tttctgttgg tgacagttgg ttcacagcta 94020 taccgatata tttcttaccg tcagggctat ctgacatacc tataccttga gcagtatccg 94080 cgtagcgcac ccaagtataa tatgttttac catctttacc gttacgacca ggaactccag 94140 tatcaccttt ctcacccttg atcttgctcc aattatagtc gcgccagtcg gtactttctt 94200 gttgtgtagc cttattagta gctataccta ggtaagcttt accggtagga tcatctgtga 94260 aacctcttcc tgagctatca tcagcatact taatccaagt ataagtagtt ttaccatctt 94320 taccgttagt acctggaata ccctgatcac ctttcataag tgcccaagta taatcagaag 94380 gatcattact ttcagtagaa ctagccatgt tactagcaat acctatataa cgttttccag 94440 tcggtgagtt actaatcccc gaacctgtta cagtatcggc gtatttaatc caagtataga 94500 atgtacgccc gtctgagccg tctttacctg ggataccttg atcaccctga tctccactaa 94560 acttagcagg ctctgaccag ttgcctgtta tgttaggctc tatacctttt ctctctgcgc 94620 tacataccca aattgattgg ccaactgatc tagctggagg ctcagttaac caacctgctg 94680 gaggtattga tttattagta ggagtagtag gttgattagt actggctcgg tatattaata 94740 ctgtagctac accatcttca cctgcttttg cagtaaactg tattggagta gaccatgcac 94800 tgttagatgc tactttgtta cttttagagt ctataacagc agtagaaatc cataccaagt 94860 cctgtgggtt agttattgga gtgtcactcc aacctgctgg cggataatcg ttacctgaag 94920 gggcactagg ttgtgtggcc gcatctttat atatattagc aacgaagtta ccttgaatac 94980 cttggtctat atctacgtta ccacctggtg taccgatctg tactttacca gatataagaa 95040 gcttctcccc gtcccatcta ataaacttgg tagaatcgcc taagtcgaac ttaaacttct 95100 ggtcattgtt agtgtaaccc atccacatac cttgtgcgga ttgtccatac cctgtcttag 95160 atgctgttct tatactagga ttatctttac cagataaatt agataataca aaagaagctg 95220 agtacattgt cttagatatt acagcaccat caacgttgac gttaccactt aaggggtcaa 95280 cagaaatagg cgctttacct tgggcatcta acttaccaat agctaatgaa cccgtttttg 95340 catctaatct aaatgtttgc tcctttctac ccgctaggtc tctatatgcc ttgatatggt 95400 cataagtaac ttctatacca gtctcattag caaaagaaga gtctagaggg gttgagcttg 95460 taattttaaa ggatgcggta ttagagtcgg tcagtgcttg atcagcaggc ccccatgcta 95520 tagcagctac cttaaattta tattctttat taaaaggaaa gcttgtaatt actgatgctt 95580 tagcagcacc actgttaatt ttgctcgcct ttctccattg agttttatta tactcgctag 95640 tctctacaaa gtaaagaaca aattctctga cagaactgcc agtaccctta tcccagtccc 95700 aacttacttg taagtcgtaa cgttctatcc catccattat ttttgctact ttaaaattaa 95760 tattctctgg agcactaggt gggcggaaca tgttctctac tttaacattt tttattgtac 95820 tataaggtga ggattctgta gtaaccccat caggcatata aatgaaacct ataaccctaa 95880 agtcatatgt accttgccct gtagtaaaag ataccgcatc tggagcacca ctataacaag 95940 gttgccagtg agtatcgtcc tcagaagaca gcttcttatc tattcttata gattgagcgt 96000 ctccttgtat atccatagtt ataatagggt caccaacgcc aatatctact tcttgggctc 96060 ctactataac attatgaatg ctagggtact ccaacgtttt tgctgaggat gcttctgaca 96120 tattaatacc tagcttgctg tccagaagct ctgagtctac catagcatca tagtaagacc 96180 cttcgatgat tttatattct gtattgtcct ttaagttatc aactatagta tataaaacat 96240 ctatatttgt atctataaat ctagtcccat ctatccaaaa tgctttacca actacgtctt 96300 tgtcctcgta gctagaatga gggatataca gttttgtata tcccggagcg atagtaccta 96360 gaactagttt tcttggtagt gtattctcaa tcatagtatc gtccaatcaa ttgaagttat 96420 attgttatca gcttctcctc gaatatatat tataatattt ctacatatac cgagagttcc 96480 gtgtgttcgt ttataatctt ccttattcat atcataagta tagatgaaag aagaagaggt 96540 agtagtggtg tctcgtaaga cctcaccagt atcaccatct aaaaatctaa gtctgtatct 96600 aaagcctgaa accgaggcac cttccagtag ctcgtcccac ataagctcta catctttacc 96660 tataaatact tttgcagatg tatctgttct atttataagt ctaagatttt cgattttagg 96720 tagtatagcg ctagggttaa cattaactac caatttagct ggtaagctag tgtcaccgtc 96780 tggggatact gctctaacct cgaaggtata ctccccttct gctaaattat aaagtggcat 96840 agtaactctt gagctcagag gagccgtgcc cgatacaggt acagtaatta aatccactag 96900 ccctgtatat ttaatagtat agtatgccac ccttttagtt aagcttggca accatgaaag 96960 agtaccgttt aaccctacct cgggctcacc taacttaggg tcatactgta ggtctcgtgg 97020 gggtaataca cttacgacta catcaggtat ctgatcatca ctattatctg tttgtgcaga 97080 gtttataaac acatctggag cgtattctct agcagttacc ataatgtgcc cattcgcttt 97140 ccaagtcata tcttggatta agaaatgctt cttgtcccac ccatatctct ttacacttag 97200 agatatatta gcatttgggt atagccataa gaaagtaaaa ggtagactaa atgaaacagt 97260 tctattatat ctagattttc ttaaataata ttcggctctt gtacgggcag tatagtagtt 97320 agttatgaaa gggaaggtta cgtttgcttt tctatccttc ccattatctt ctatcttata 97380 atctttattg aagaaggtaa tagtattact accccatgca ctagcaggat caattatact 97440 tgcttggatg gagttatact tgttctccat agagttgtca gagactttga tactaccatc 97500 aattatatct ccttcattta tatcatatac tactggctta tctgcctcca tagttagagt 97560 atacttacca ctaactatat tcaaggaagc atcgtattga gcaatcaaag attgcacatt 97620 cttaaacaca gtatcactag tagataggat agcggatcct tgcagtattt gtctattttc 97680 tgctgtataa tcctcccaac ctaggtatcg ccaataagga acccagtcag ggtcgtatga 97740 agtatctact gcatccatat acttagcaca ttctcttaca gaagccaagt caatctcagc 97800 taaagtaagt cctgccccgt aagttgtact acgtaaatag tctagtgttt gccacgctag 97860 attcaaggag gtcttatccg cggttactgt cccatcttca tggtatactg gtatcttctt 97920 accttggatt tctgcggaca cttcaggtat tgaggttcta ttttcattaa gttcgaactt 97980 aacaactagg taagcagtgt ccgttagctt aaatgagtca tcccagtact ctgccccata 98040 gttaccatcg ttttgtaact tgaacgaccc tgctgccgct ttgtccacaa gtacctttgc 98100 agcatcctgg gttagtgacc cgtggaacgt ccagaagtct ataggcccgt taccatcatc 98160 atagatatag tgctccccat gcttactagg tacggttctt ccactagcag gggccagagc 98220 tcctatggtg tcaccgttat tacgcttcat acctatgcag gcgcgttttg attggtcagc 98280 ctcagatata cagatcattg gattatctcc aaaaaatata tctaggaacc catctatctc 98340 gccctcacag aatgcatata cagcccatat ctcttccggg ttattaatat ccgtatcaaa 98400 gaaaataggt ataccggcaa ccttctgtac cccgtatact acaggtaaat atttggcagt 98460 aaggtttata tccatatcta cttcttttac tacatttgcc cagtattctt ccatgtcata 98520 atgtttttgt cccataagac cacggaagcc tccagcacgt cttttcttta gacgataacg 98580 tttttctttt gtctggtatt cagctagcag cttgatggac ttattggcgt ggaagaagcc 98640 tttatctaat tgatattctg gctttttagc gctgcttgag ggttgcatga ttccgtttac 98700 attgatcaat cctcggtgag acgcatcgtc cgtaagtctg ccgttaatag cgtcaagtgc 98760 atataactta ctagcacagt tccatgtaat tacagaacgc cctttacctt tattagtatt 98820 tgactcgtct aatgcactac ttactataac cccttcaaag taggtaacag tactgccatc 98880 cgcatatatt tgaataatct caccatcttc atctaagaat actctatcta tttttagttt 98940 tctattcaaa aagctcttag aatttagcac tctgtctatc tcttcatcta tagccccact 99000 tacttgtact gggactctat agattgttag ctctttggtc tgttttacat cgcctactct 99060 tttgattact cccgatttat atacttgacc tttataagta atatctctaa aataatctgt 99120 taagtatata aaaacacctg aactgccagg tagctctagg gagactagat gtgctaaatt 99180 tatttcttct gtttcttcta tatacttctt ggctgagtct aatatattac tcataagctc 99240 tccctaaatg aataactgat attttcgtac ataccatctg catttacgtc ttcggagata 99300 gactctctac ccattagttt gacttgcaga ggtacagtat taaacacggg cttttcattt 99360 cccgtggtct ctataaataa atctggatat atattaagaa taaatttatt atcttctttc 99420 ttaaatcctg ttatcttata tactttagga tgtgtactaa gtttaaatag atcacctagg 99480 taaggtgctc cttgtagaga gttggtacca gtaatttcta tagtactacc cttctgacct 99540 gcagcaatag agcatgcagt cagatctcct agtatcctaa aattaacata ctgaggtaga 99600 atcaactcaa tagagtcccc agtccttttc gcctcgttta tcgcagaaga aaccaaacga 99660 tactctaatt cgtataactc tgggtatgta atattcacac tccagaattg cgctgaagtt 99720 tttgtttgaa gcacttttcc gtttgggagc tcatcttgta gaataggctc attatctacc 99780 aacggagctt ttaagaatcc tggtgcttca tcaggatgtg tgaatggatc aggtaatcta 99840 atagccatta ggtgctccaa atgccattca acgcgttgac tggcctaggt cttttaaagg 99900 aataatgaat ctcttcatct cgttatatag gtaattatac acgtaaataa tctaaagtca 99960 actcaaattt tataaaataa aaatgggcta ccattacagt agcccattat acttaatata 100020 acttgtcagg attcatgccg ttttctttgtg cggaatctct ccatgcagcc ataatatcgt 100080 ctttcctttc taagaatgat ctagcatcca tagtatcaat attaaattga ggggcatagg 100140 tgtaaccacc cttacctccc gcagctgatt cctcagcact gtatgctctt actggctcta 100200 ggggtgtgat tacctctggc ccatcttcac ctactacaat gctattacca ggagtaccta 100260 taccaccatt tgctctagga gtaaatgatt gtaagctgcc agtacctcct tcatttctta 100320 tgtaagaaag ttcgccacga gtagctgatt gagacacatc cacctgatta gacctatcac 100380 caagagttag tgaagcctta gtatctcctg cagccccaga aactaagtta gcacttgcac 100440 tagatgcttg ttggtatgcc agaccacctg ctaatgctgc tgaagccatt aacgggatag 100500 ctggaacagg ccaggggtta gcagccgcat tcattaccgc taccgcagta gagataagga 100560 tttgttgttt agctactttc ttctgttgtt ctactttctt cttctcaaga gctgctattt 100620 tagctacaga ctctttagac ttaccgtcac gtttttgctc agcagctatc tgttgatcaa 100680 tagctccaat agcttgactt gacgtgtatg acaacatacc ttggaaggca gttagtcctg 100740 aagataccat ttgggtagca gtcatactag actggcccat attgttaaat gacattgcta 100800 gcccattcaa tgagcttgtc atttgatcaa taccaggagc aaaggatgct agattactaa 100860 gagcggactc catcatttga tttgattgag ctgtctgagt agctaggatc tcattttcga 100920 gctgttttat tctattaaga gattgttctt gttctaagtg agccccttgg atcatctgta 100980 gattggctaa tctagcacgc tcattagtta gtaaatcttt cttaattaat agttctggct 101040 gcccagccat actcaatcta gtttgagtat caaggcgctt attatgctca tctactaaag 101100 ctacctcagt attgagttct tgcatagata ttttagctat ctctgcttct gtcttagcta 101160 gtgcagtagc atttgcacct agatttctct ggtctactaa taaaccggtg tatagctccc 101220 tttgtcttct tagtaattca agcttaacag cattaggatc cttacctgag taacgtccct 101280 gaatctctgc ttctgcctga gttttagcaa agatggctaa ttctctatta gtatctctgt 101340 atctcttagc taatctttct gctttgacta gtgcgttttc agactcaaaa ccaagacgtt 101400 ttagtgcttc tgctgcatca aatgcagggt ctgctgcttt aagttcgaag taggcattct 101460 tcatgctctc tatatcagaa tataatttct cgtaagatgc agactgacca cggacactag 101520 caatactaga attatatgat tgtaccgtgc cttttacagt tgaactaaga tttttatatg 101580 ccgtatttgc tctgtctagg tcttccacag tacctacaga gtaatcttgc gtcattattt 101640 ttagtcttgt caaagcactt cgtaaataac ctgctcgctt agtcaagtca tccacactat 101700 caccagcttt agggtcacca agagtgtcta acactgtctc tagttctttc tgatactgtt 101760 ttgtcaggtc acgtacttgt actagaccac caatcatacc tgatctggtg tcctgttcaa 101820 acgctttagc tagtgtttcg ctaagtagct gtgctttggt attggcttct gataatactt 101880 tatccccaaa catccaacta ttaaagaagt tagccatacc cgaagtagct tcagccaagt 101940 tctttaagaa tgtttggaaa gctgtgccca tgttagcacc gaaagtttcc cacggagcac 102000 cattacgcag catttcatcc aagtcaccaa acttctctac tgattgcgca ttaatctcat 102060 taaccaacgc agcacgttgc tggtaagagt ttagggagtt agcactaaca cctatttttt 102120 cagcataacg ctcataggct gtggtaagtt tagttgtaat accaagttcg tctagtaatt 102180 cgatttctag ttttgaagta ccacgaatta cacggttcat agcgtcctgc atatctacac 102240 ctagtgcaat agatgcgcga cgtgcagcca tagttagctg ctctatttgc tcactatcaa 102300 aaccataaga tgcagccgca gcagcgtttc ttagagcctc accatagttt agtgtatgcc 102360 ctgttagctc ttgcattttt actgcggtaa attggactgg ttgtccaatt tgagagccca 102420 ctatcttacc tacttcttct agacgattta attgttcgcc ttcagttatt aaacggaaag 102480 cttcgctaag tgcgaatacg ttagcagcaa tattagcata tagtagtggg agtgggccag 102540 caaacttagc tatatctgca aatgtacgtg cttggtttct accttggcgg ttctggttac 102600 ccataccgcg cccagctttt ttagcccctt tacctacacc tactaacgcg tcgttagttt 102660 tacctgcctg ttgacgtacg tctataaggc catcttctac tctttctaag tcagcaccta 102720 ggctattagc tagattatcg aaagagtgta tcatgttatt gttcatctta ccaatagtag 102780 tatctacatc ctgtatttct gataacatag aatgcagtgt ctctactaca tcgtctaatg 102840 aacgttgtat agcattagaa tcaaacccaa agtttatctt actagtagat ttagctgccc 102900 tgttagctgc ctcttctacc tttttgaaca tctttggaga acgttcaagt gaagcattaa 102960 aagcattatt agcagttttt gcttcagtaa gggcgtcacc gacaccctta atagcttttt 103020 ctgtaggttt agcaccttgc tgcttcacct tgatgattag ttcttctatt agcttattag 103080 ccatataaac tcccgtttat tagcttagtt ttacgtgttt gatgtagttt ctatcgtatc 103140 caaagcctgc tgcaacttgt acaattaaat cgtccttacc tttacttgct tcacgatctt 103200 tttctagact ataaatagta aatcctaagg ctactccctg tgtgtgtagt actactagtg 103260 aattatcatc cataccgtaa agaggtaata gtttgataat accttctttt accatttcac 103320 ggtgcgcatt atccatagta cctagaatag taaagtatgt cattggggat accactgcta 103380 ggaactgccc ttcgatagca agcatttgag aagggaactt cttaatagta tctacaatag 103440 tagcaaaagt cttagatgaa gcacctgatg ttggtgtagg cgtagtcatt ctatctgcca 103500 cataattctc taacttaatt ctagcctgag aggtaagaat cttatctaga gctacttcca 103560 tattagggtt agcatcttct gtggtctcgt ctactagagt tgcagatgct gctagtaatt 103620 ctttttgtac ctggataccg cctacagaga agataacgtc agtatcgtta ggtaagtgaa 103680 ggaaaggaat atcagtgtac tttcctggta gggttacttg aggtagtagt ggtaaaactt 103740 tactaccttt taaagtagct tgtcgtaatg actctacttt agtgtcttct gcgcctgaca 103800 cagaagatgt tgctgatttg ttcacggttt cgatatatgt ttttaggtct ttaatcattg 103860 ttttctccat acgaaaaaag ctctgggcat tacacccaga gcaagctcgt ggaatgcccg 103920 tgaggactct gttaagtagt tagcggtgtt ttgctttagc ttttattttc ttagcctcag 103980 tgttccattt gctaactgct tttttaacca ttttactgtc tacgtgttgt attacttcca 104040 tactaattt tctatcctcc atatcattaa tttgatatac atcataaaat aaatcgagcg 104100 tattaaatc tttcccgata tatagaggac ccaaatcagt actagtaaat ctgtcaccta 104160 gtctgttata aatttctata gcatccttaa ccagacttgg aaagtcagag aagtctactg 104220 gaattctttt aggatcaggg gtctctccta gttgagcaca catttttagg tacttacttt 104280 ttgagaaacc attctgttta tcgaagtatt tgtctagagc ttcgaatagc aactttcgtc 104340 gtttagtctc gaaagttgtc tatcaacgcc agcttactag taacccagtt gtcaaaagac 104400 tcagatagtt tactagttg gatagctgtc tccacatcga attcaatttc tgcatcaggg 104460 tcttcgacct ctttggtatc aattaagata agttttgcca ctttatttaa ggttagccct 104520 gaccatccat taatagcaga cttaacatac tcttgagtat acatgtctac gtctaagtcc 104580 atataaggta gccctgactg atcgtcaaac ttctgtacct tacacttatc ctgaagtttt 104640 cgtctagctt ctcttgatag gtaagtaagt tttaccttaa agcccggaat tcctgggaac 104700 tcaaactctg atgtttttga gtctaatgct agtgctgcta aatttagctt catactaaat 104760 atcctccata ttccacaatt attttatttg tttgatctgt tacccttata tcatgtgctt 104820 cagtaaaaac gtcagcaggg gcaactctat gggttactaa tgcatccctc atagagcata 104880 gtaaacctga tttatataac tctaaattag aatagtgaga tttgtcagga gttgggtgta 104940 tggtattaa gtatacgtta gtattaatac caaaacttct gtctgttagt acggggtgtt 105000 tagggatata taaactacct gtatcatgaa gtgacctatc ttctcgccaa cttatagttt 105060 gctgtatcga gattcctgca tttataatag aatttcttat ctcattatct aatttatatt 105120 gtataggtgt aggttttagg ggtgtacctt gctctaccat tcctgcactt aaatcaatac 105180 taggaacctt atctatagag ctagcagtga acgaggtttc aaaagtaagt gcttcactta 105240 acgacagtga tatacttaaa gactctagtg ctgctgtttt tactttgtat accccttcag 105300 tagtaactaa gaatatgtca catatctctg gagatatatt taaattatcc tcataactat 105360 acacgctatt atgtattttg gttaatcctg ctagttcaaa caaaacacct tctgcaaaac 105420 tttgagtgcc taaaatgctt agactgaacg tagccgaatt tttagaatta gcaaaagtat 105480 aaggttttgc tcgtcttgag tgcaatgttt ttcttgaacc agaggtttta ttaaatgtct 105540 gactaaaatc aagttgggtt agggcatcga aatgataccc tacccctttg taaactaaaa 105600 caacggtgca ctcttttagt agagtgtatg ccatatttat taactaattg cgactgtaac 105660 tacattacta actttcttgt cgccgtcact attatatact tcaacgtagt agttaccagc 105720 atctgcagat tgtgcattat ccttagtata ggtagctgag gtagctccac taataacagc 105780 accatcttta taccactgat aacgagtacc accactagcc gctactgtta attgtagttg 105840 ttcgccacta ccaagtacct gattagtagg ctgtgttgtg attgtaggta gcggtgctat 105900 attctctcca gcatccccaa acctaactag tgctctaatc tcatcctcag taaaagtatt 105960 actcatactt aagtaaactt cattacctgc atcaagcgct gttgggatac ctttaaactc 106020 gatactagta cctaatacgt ccgctgtttc tgtagtaggc acagaaagat gggctgtagg 106080 tagcactact actacccctt cagcaggagc agcataaacc ccgcctaggc agatacctat 106140 ttggaagcta ttggttatat cgcgagccga gatcatttta tttaacaact cagcagctct 106200 atctttttct ctaggatcat ctccgtctct attacgtaag tatgcttcta aagtaccaga 106260 aacttcaaat gtaccagtaa acgaaccaat aggcaggtct aatctagata gagtattagg 106320 ggttaggtaa ctgatattgt tattgatagt aatagtacca ccagtaattg gaatatcgta 106380 ctccttacca tctttgttat ctttaattaa tagagtggta agtttattct tgatgtattt 106440 acctttacct gcaccgaaca ctttatcgtc atcaaatatg atagtacttg gatcgaaagg 106500 ctgcgcagta ccaagaggct ctactttagt accttgacct gaccatgcag tcataccaat 106560 accttcgata tctacactaa tctctgcttg gttaatctgc acatcgtgaa tcttatacca 106620 tacattatct acttggtagt atagggtaag agtattaagt tggtggtatt ggttatctgt 106680 aaacttaacc aacatatttt tggcagtagc tttaactcca ttatcatctg taatatctaa 106740 aggagagcca ctagatagac agtgccacaa tgcaaagtct ggtgtaatct taactgaatt 106800 ctcctcataa ggaatcaagt atgttgagaa gttccagtca gcagggttta gattatcatt 106860 gaatctctta gaaccacgag ttggacgtgg gcctgcttca tctaagttaa tgtcggtaga 106920 gttaccgtct tggttaaaag caaagtcttc ctgaataagt acttcccaag tgttagtatt 106980 atcaatggta tctccaggct ttgctacact agcccatagt ctagtgttac gtaataattg 107040 gactgacatt tatagtagac tccttatctc ttaaatctaa catttctgtc cgggtatctg 107100 atttcgatag ctacttcacc tatacccatt gggtgcagta taccttcatc tgtatttata 107160 ctgattacgg tcatttgagt agcttcgtat ttcttttcag ctccctgagg tgtagttata 107220 gtatattcta tatcctcgtg tgtgtcaata aaggttttta tatcttgaat tagtaactct 107280 aattcttcgt ctgctctgtc ctcggactta acgtaggctc ggatggttag agtaaggaac 107340 atccacctaa acccaccagg ttgatactca ggtctttccg gtccgaggcc tactgttata 107400 gcaggaaaag tttctacctg atctagatat agatttttat tgtgtacatt catatctata 107460 tcagtgtagt acttatctaa gttttggtca gaaagctctt gcttcataac ctcaacaatt 107520 ttgtctctta tactagctct atgtgtcata gtaacgcctc cctgacgtct atattatatc 107580 tagtatggat tatatctcta gcagcttttg ctaacgcctc tccgatatgc gtttgagggt 107640 ttctagcacc gaaggacggc ctcatgtaca tagcaggtct agtagacttc atagggtcaa 107700 atgtagcata tgggtatgtc atataagaat aaaagataga tagttgtggc ttcctacctc 107760 tatcattgtc ctgtatacgg acagacctaa catctagaga gttggcaaac ctaccagtac 107820 gatattttaa gggagcgttt ggcttcttca tatctctaat caagtaatct ttagctacta 107880 tttctagtag gccttttaga ttattctcag aaataagttt acccctgatg gtatttatac 107940 ctacagttct accttcttcg aggtagtctc ctaaccttac ttgaactgag cctttcgaac 108000 gttttctacc gacactgtct gctaagccgt attgagctag tattgcatct accacgccta 108060 actgtctggc gtctaaatta tcagctactc ttcttagtac gtgcccacct ctttcacgtg 108120 cctcattttc cgctagaggc tactaaatt taaaagatat agtagctacg ttattatcag 108180 agcgagaatc atcagtcgtt acatcataac gtacctgtct cttattacca cgcttggccc 108240 tatcatcgag ctctttatgg attatttgct ctaacatatt agacattgcc attagaacat 108300 cctgtgcatt aatagtatgg ctctgacatg agggggggaag tttttacctg tctggtaaga 108360 tactgtctga ccacctgctc caacagatgt tttattaaat tcttctttat aataatactt 108420 gactagcaac atcgtagcta attgaatagc ctctagctct tttggagcta cctgatagga 108480 tactagtaaa gtgtcaccat ctttaggttt tacagtatat agctctagta gtcctgtctc 108540 agatacatta taatctgcaa gagtaagttc tttaccatct gctgaatcat aacctctagg 108600 gatatacttt agttgctgaa catccatatc aatagctggc aacatgtacg aggtggtatt 108660 attacgtgtt ctgattgtag tctcaatagc tccagtagat gttaatgggt agccgatata 108720 atcctgaact atttcagaac aagatttgat aagagcctct atttgtgggt cgttcttctt 108780 accttcaata ctagcaaaag ctttgtattg atctattgta attatttctg ccatcttgtt 108840 ctccataaac aaaaaggagt ggagcaaatg ccccactcct cctaaagttt gctattaagc 108900 gtatgtacct gcaactacgt tcttatcgaa gtaacgttgt aggttcaagc gttgtgtaac 108960 gtagtaagcg tcacgttgtt cgcttgcacg acgttcgtat tcaactgtta ctcgacgttg 109020 acgaggaact acgaactcgt tacgatatac aatcgcacag aaggctgctt tagctgcttt 109080 agctgggaag tagctagaaa caactactgg cataccgtag attcgaccta cttgaccttg 109140 tagtttaagt gatgcgttag cacctacttg gttaacatct tgccattcgt catcttctag 109200 aagatcgtag tacgcgtcca tagacacgat tagagcaaga gcgtctagac gtagaccttt 109260 aaggcctagg tacgacgta gttggtgaat cattttagcg gttactttag tagcaccgtc 109320 atgagtagct gtagtagcga ttagtttgct gtcgatttta gcaaagtcta gtagacctgt 109380 tggtttacca gttttagcac ctgtaccatc acccatgaat gctttttcga tagcttgtac 109440 gtgagcttcg attaggtgac gacggatgat tggtagaagt gatgtgattg catcttcttc 109500 tgtttcatca gtcatgtaag ctttagctgc tagtttgaac gtactgaatg taatatcagt 109560 aagtgcaact ttaacttcgt tacctacagt agtgtcttta ccgtaagttg atgcatctac 109620 ccatgttgca tcttgtgcat caggttcgat ttgcattgta agtttcttac ttgacattgg 109680 tagttcttgg aacattgcac caacaactag aagtttctga atgtcacgta ggatacggtt 109740 tgagaatact gtttcgtagt tttcgctaga aacttggata gaagagctat cgtttaccgc 109800 tttctctagt ttagcaccaa attctgtttc gaatacacct ttcttagtca tgaaagatag 109860 aagtacagct ttttcagctt cttcttcgaa atcagtttga gtatttagat taaatgcctt 109920 accaactgca actgctacag aatttttacc ttcgcgtgct gccattagtt gagcgatttc 109980 gtcacgtact gacttaattt cgttttgtgt tttttctact gatttagcga aaccatcaac 110040 atcaccggac tctagcttct ctgatagagc tttaactagt tgtagtgctg tttctagttt 110100 ttctttttgt tcaccagtag cttcatctac aagagcctta acgcgtgctt cttcagcttt 110160 tcgttgctct gctagagctt tttcttttgc ttcctgctct tctttcgcag ttagcttatt 110220 agctgcttca attactgttt tcagacctag tttttcttgt agttctgcaa tttgttctgc 110280 tgtcattggc ataataaatt tatcctttaa taactcctag ctcgatggct agcttttcta 110340 acacgtcaac ttcgttagtt tcgctcggct tgctagcctt tgcttgaagt tgttttctaa 110400 aatcattata atcagcagag ttcaagctct tggagacttc gaacactgag tcctgattac 110460 atggaactga aactactgat acctcaaata gttctagttc tttaattaag taaatgtctt 110520 cttctttctt ccattcagca tctaggcagc ggaaacctac actaaatgtt ttcaaaacac 110580 catctttgat aagctggtat actgggcctg ctgctttgct aatctctgct tcaatctgta 110640 agccttcgtc agttacatca taactaacca tcttaccgat aggcatagcg tgattgtgga 110700 atgcaagaat gattgggttc ttttcatagt taggtagggc atttgggcct aaccatgcat 110760 ctttagggat tacatcacca gcacgatctt tcgttactgt attagcataa cccttaatta 110820 tcaacggaga atcttcttcg tccgaagctg ctttagctac gaaactagca gatagttcca 110880 tatcaatagg cttatttaac ttcatccttc ttctccttgg ttggttcagt cttcgctgcc 110940 tttagactag ctacaaattc agcatattcc ttttggaata gcttgaatgc acggcagtca 111000 ctatcaacct tataacgata tcgaagcatt actctagaga cctcattatt agaggcatta 111060 aagaatgtac ttttatctgg tagtgtatct ccaaaatcat tatatacagt attaaaatta 111120 acttttttag ttgtcatctg agtccgagtt tgaaggtttt ccaccctcct gtcccgatac 111180 cccagtacca gaacctgcaa tattctgtgg tatacgaatc ttatccatat cctggccttc 111240 tagctcttca taacgaagtt ctttacgacc ctcattaggg gtgataagac cattgtttac 111300 tttagaagtt actgcttcgg cttctgcctt agcatctggt gctagtgcag taatatcatc 111360 tgtggtaagt ttaacatcgt aaccaaagaa tgtctcgaat actgactcga acttacgcat 111420 cataggtaga atcgtcatat aaaagaatag ttctatatta ggacgaatat tagcattgtt 111480 accagaatct agtagcaatg gtgggatacc tagtgcgata cacgccttac gctcaaaatc 111540 atcaatgtct gctttaacat ctaaatcctt agagctagta ggtgtaagtg tcttagcttt 111600 catacctgca tctagtactt taacactaga gcgaccagta cgagggttat aatctaaaga 111660 gatttcttcc tcgaaacgtt tcttcaattt cttgttaaga acagcttcag tctctaatat 111720 taagctaaac acagcgccgt tatcaaagaa tttctccttg aactgtagca gtttgtctct 111780 tctcaccaag ccttctaagc tagataagat tctagagaat ccttcaatct gagttgactg 111840 acctctatta taagcattat ctttgataaa gataatctca tttggttgga attctacctg 111900 cgcgccataa atatatctat tgacatagtt tttcttgtca gcgtatactt ccatattagc 111960 tgcgggtaga tgatataatg agtaaccatc ccaatgaatg aacgcccaac cttccatgat 112020 aaagtccatt ataactaaac gccagaacgt actagcatcc ataaacggat taggtctaac 112080 attaagtaac tcttttactt tctcttgacg aataccaccc ttggctttac cagcccctag 112140 tggagtaaaa gaatatgcat ccttaatatc atatctaact tgacaagcat tatcaactaa 112200 aaggttaaca caacggttta caacctcaac gtcctcaaaa gcacgagttg ttgttaaagg 112260 cttcctatta ctagttgtag cacttgggtc ttccctagca ataagttctt gggctgggtt 112320 caatttattg actaaccaat ctcttaatcc catgtttaca ccttgaatct gtctaaagat 112380 actttctcaa ccatcaggtt acataatgaa gtcatatcat aacctgaact agttggtagc 112440 tcttcaacag tgccaccttg cctatctcgt tgtatcttaa cccatcttct ctgcttgcta 112500 gcagttgata ggtcaggctc ttgagaatat atctggtgta gttttacgtg atgaccgttg 112560 caaagggtta cggtatcctc gactagttca tgccagtgtg ctttatagaa gtcgtcccgc 112620 atcgcgagaa cttgttcgtc agttgagatg ggaataccat tctctttggc atactttttc 112680 aatagtagag ataccgtatg gtagtggtgt agctccaagt tgtcactagt accacaaatt 112740 tcacaatgat ctttcttgga atagttagac ttaataccat cgcgaatatg ttttgttgct 112800 actcgtttgt tggtattagt tgccataact tcttctcctt aacttatgtg attagtatat 112860 atgatatgaa tgaaattgta aagtctgttt tataatttct actcggctta tctacagtat 112920 gagtagattg catatctcac agcatccgca atatgtgagt actggttatg aagtgggcgt 112980 tctttcaaga gcccttcttt aggatcccat ctgtagttaa taaacatctc acgagttaga 113040 ggacaattaa tatctactat gattttatct tgctctatta gagctgcgat ataattaata 113100 ccatcattct gggatttctt actaggcata ctagcaatat cgtattcata ggcaaggtca 113160 gcacggaact gagctgctgc cgagtcacag aatatcatat ctacatcata atcatcaatc 113220 tgttgttgga acttctctgc atgtacacga gtagtcttag actgtttctc atactcatct 113280 ataatataga atttatcttc ttctaaatca tacttaataa caacactagc tgttgggtct 113340 ctgtacccat ggtcgacacc catgatagtt tcgtattgat tctcatcatc aaagtccatc 113400 tcactaaggt ctttaacatg tctgtcaaat acaaaccctt catagatacg tccttcgaat 113460 accgagaagt cagcttcgta ttcttgacgg aagtaagcat ccgagttagt gatacgagct 113520 tgttcaatat cctctgggct agcacgagga ttatcacgcc aggtaccgtg gattgcaacc 113580 cagttagata atgcagggtc tgttgattta ccttgctcga agaatgtctt aaagtagtta 113640 gaaccacgag gtgtgctgat aaagattgcc ttagagttag gtttatccag tgtaggacgt 113700 agagcaacct ggaacgcttc tccaccttta tctgataggg cagcctcatc aaagataatc 113760 aagtcatatg aacgacctac tgctgagtca gcacgttcag cagatgctag cttgattagt 113820 gtgttgttag atagaagaat ttctctatct ttagcattct ctttaactgt cttcaatcca 113880 aattgtttaa tatatttctt aatctcaccc caaccgattt cggctagtga gtagttaggt 113940 gcaacgacta gaatcttatt attaggttct aatgccttta agaatgctag ggcgaacgat 114000 ataaatgact tacctacacg acgtgataca catgctacca cgaaacgatg cctagggtca 114060 tttacagcat tgataatagc tatttgtggt ctattcggta cgattggact accattatct 114120 gctcttactt gctgtataaa attatttaca tcaaatgcaa agaatctttg atctaatgga 114180 aattcttcta tataatcaca atttatataa tctctagaac aatacattat cccaccaatt 114240 tattgataag agccatgtag ttctcatcct caccgccagg tatgttaata ttattttgaa 114300 tgttggtttg attagttgga ccacccgatg ctgcagtagc tttagcacgt tctatatcca 114360 tcttcatttc atccatcttc atcttatgat atactttagc tagctctagg atatcagcac 114420 tagtaccaac acctgtttct tgtatttctt caagcttcat attcataagt tgatccataa 114480 caccaaagat tctatgtcta tttctaaatc cttgttcatg gaattgtcta tctatatatg 114540 ctttaacttc tctattcttt aattgggcat ccacttctgc cacaggaagg tttaattctt 114600 gggcaacctt cttagagtca ccaccgaatg taaggtatgc ttcagcaacc tgtagccctt 114660 ccggttgtat aatatctggt agtagtttat ctgtcatact cctctccttt tccaactaat 114720 ataacatcaa caatcacaaa tgtcaacaaa aatttaaaaa tcaaatttga caaagcactc 114780 aaacccagat ataatagtac ataaaaggag gaatattcat ggcattattt atgggcagaa 114840 agccctcaga taacagacca ctactacatc tatctagcgg gaatgagtca gtctcacagc 114900 tagacggaga accgatagca tcaacgctat tccactctga gataccttat ttagcagtat 114960 atgatgtggt aacaataact aaattcagaa aggtaagatc agagaacaca tcttcggggt 115020 ggactcatta ttatgaacct atcatacctc aagaagtttg gaactatata gataatagat 115080 atataatgac agcttcttta gtcacacatc acccagacta tgtcaaacac caagtaatgg 115140 gtagggagac agacaagcag accagaccat acaatttatt cgtaggtaac ttcaggggta 115200 gcttccctaa tggtatacca tacccacaat ctccagtacc aaatactggt actatctgga 115260 acttatggca accgttaagt ctatacaaac tagccgcaac accaaaaaac agagaccaag 115320 gtgtagctgt acactatgat tataatggta agtggtcagt ttcaggacta cagaaagaaa 115380 ctactagtgt atctactatg cgtactatat atgcaacccc taatgtttct aagtcgtcta 115440 gaagtacatc ggctaactat accaatcaaa atagagatat aactaagcta tttggtatta 115500 gaggtgtagg cttacctttc atacgaaata atagctcgtc agcacctagc acggttacat 115560 actcagcatc aaaaccttca tactataact ggaaattgaa agtttatata tttaatgtaa 115620 aatataatgc tcagaataac gtcgagcatg ttagaccaaa tatcaaagat ggtataagaa 115680 tcgacaataa ggatatggtt attggtgacc tatctatgaa gagtggtatg cttatctctg 115740 accctgagcc aactaaaaag aaattcaaaa atacagataa taataagaag aaatcgtttt 115800 ttgataatat agctaagaaa ttaactttag ctcaacttac agactacccc ttcccactag 115860 cagatattac tgttcgtagt aataaaactt ggagtataga atctgactgg gcagtattac 115920 aagtacatgc tccatcacca ttgctaggat ctaatatatc gctggaattc agtgctgacg 115980 agattaagta taaaggaagt aagggggagt tcttaatagc atctaaggct agaaaatata 116040 caccttacat cctcggagga gatagtgcta tcacggcggt attcccggca gaaacaaaaa 116100 catttgctca actaaggcaa ggtacacggt ttgttaagac taatatacct gtacaaggtt 116160 acgtaagggc tgtatataac cccaatgaga ttggtaacta tgagaaggag gtagttaaac 116220 agagaatcaa actaccaccc ggcacgacat ccaagaatac tgtaactgca catatatctc 116280 ctctatactt gcacggggag gcgagcagca cgcttggcag taaaaagtcc aagatgcagg 116340 taagcctaaa cgggtttgca gggttctcta acctaacact agagaccttt gaaaaaagtg 116400 aacctaataa ggaagttata gtaggtaatg tagcatatag tgtaatcaag gatgtatcct 116460 tatcaagaca tagtgaaggt agaataggct cctcatattg gccacagagt tctacggcaa 116520 ataaaatgca ggctataaga atccagtata aggtacgggt aaactgggca agaaacgaac 116580 tagaactagt aggatattac aagctaacta atgcacagat tagggcaaat gaaggaggta 116640 actggaatac ttactacaac aagttgggtg agcgggatgt tacatacaga ataccagaag 116700 ttcgtttcgg gttctatgta acgggtacgg cataatttta aaaaattaaa atttaatttt 116760 cggcaaatca gactatgttt tcggcatagt ctgtttttgt ttatatagca ccttagtcat 116820 tttctgaaat ttttaaaata gttacgtgat agggggtgta cccccatagc caaatgagaa 116880 ctagtctcat taccgccccc tatatgataa tggctctcat ttgagagcca ttcttatttg 116940 tacagctttt ctttgattat tgagctaaaa gctcatcaat ccattccggc ttgcaaatca 117000 cgtcttgagt tgagcattca tctattaggt cttgaatcaa ctcggcggtg tcttgttcac 117060 cttggcggta aatgtctgcc cattgcatct gtaacagctc attcatcaat ttggcatcgg 117120 tagctgtaac accttttaat tgattagcca tttctaatat accgtcgtgg tcgtcaaaga 117180 aacaccactc agtaacttga ggcatactag caaacaagtg attaaaccat tcatgcttgt 117240 atggcgcgtc tgacattgca ctgatacggg ctgcattgcc ttcaccaaat acacgcggca 117300 actggtcacg gctgcaaacg ataggcgcac gtagtttgtt tttgcgtagg taaacataat 117360 catggttaag catgaatcgt gcggtgagaa tgattactaa ttcgccttgc tcaattagtt 117420 gttgcatata tccggcaagt ggtaagagcg tatcatctgc gactttctca ggtgtgcaag 117480 cctctttaat gtatttgtca aggtttaagt tgccttgtga atctaggcaa ggctcaacac 117540 ggtggaatga atcaattgtt gtgccgtcaa ggtcgaatac atatgctttt ttcatgaata 117600 aaatctccaa atatgggtaa gttgtaaagt gtgacgggcg tcacatttgc gcccgtcgag 117660 ccgttaggct aaggattagt attgttgttc gccaatcaag tacataggaa taagttgaca 117720 agttgagttc gggtaaacaa tagcgttcgc tctgtcccaa gttgtaccgc ctttgttgta 117780 acccatttcc atactaccag ttacaccgac aacaatattg ttgttatctt cgaatggtga 117840 gtgtgtatgt cctgtgatgg ttttgattcg cattttctta aagctgacag ccgaaccacg 117900 tgccccacct gtaccgacat ggccgtgcat tgaaatatca taaccatacg ctagcttttg 117960 ttcgtcaatt ttgccgaact cgatattctc gctcagttct ggcaattggt cggctagggc 118020 tgcgaaagct aaatccattt tcgctaggtc gttaaatgtg ccgttgtcca ttgcttcaat 118080 ataagcaagc attagggcat gatatgtacg ggcgtttttc gggtcttttc gtacatcgaa 118140 tttattatca cgtagccacg aatcaagggc taaatcatgg tttgactcaa caataaagac 118200 gggcgcaatg tcagcaagtg cattcaagtg gttgattaca tcggttaaat cttcgattac 118260 tgcacggtct tgcatctgat acaagaatgt ccagtcctca cggttgtggt ggttacggct 118320 cataaagtct agagcatcat gcacaacaat tagtttagga ttttgagcga atgcccaatt 118380 tgtagcacga ctaaatgagt cgtcgcacat tttttcgcaa tgcaaatcgc ctagcacaat 118440 tacgggctga ttgtcaaata catcattgta agctttgatt gtaccgtcgg ggaagtacac 118500 cgaaccgtta tcaacaataa cgccgttttc gtctgctact aatcgacggt gattaaccat 118560 gccgttagtg acttgaatca ggataccacc gaaacagtgt tctgcttctg cttctgcccc 118620 tgcacggctg tcagtgtagt gtttaagcgt acaaactgcc gttgtataaa cccaacgatg 118680 tgagccgttt ttctggcgag gtagggtttt tgtttgtcgt ctagggcttg caactactgt 118740 cattgactcg ccagtattta gcttttcggc tgcattaata ggttgttttg cagtcggtag 118800 aatttgagca ctgttagcca ataaaacgtt accttcaccg cccaaccatg tatcagtata 118860 atcaagtaaa tgctttttga tttctgcgtg gaagctaggc tttttacgct ccaaaccttg 118920 caacgttgtt gtgtatttaa ttggcatgat accgaactca gcatccaacg atttagccat 118980 tgacaacaaa gtatttaata ctgggtgtgg gcgtgtattg ttttgggctg ctgtgatgat 119040 atataaacca tcgtctaggt tttcataatc acgcccttgc aagctacctg caaacgttgg 119100 ctcaatatct tctttcgctt gctgactatg tgattttttc tgcgtcttgt ttactggttt 119160 gttatcgtcc agaaactcga ttagcttact agaatgcaca tcaattttaa gagcgtcacg 119220 gattgagatt gcaacctcac gacgttttga atcgaggcgt ttgccccaaa agttctcgcc 119280 ttttgctagt ttaaggattt gcgctttgtt ttctactaat acttttgaca ttgtttatac 119340 tccactatgg ggttggttga aatttacagt gtaaactgtc catcaattta cactgtaaat 119400 ttacgcccta gtttaagggc gtaaacccgt tattagctaa atgcctttag aacttgaacc 119460 ggaaattcac gttcaccgcc actagtgaca attgacataa tatttttacg ctctgttagt 119520 tcagtgattt ttaacgcttc acataacaga ctgaatacag ttagttttgc cgctttatta 119580 gcataaacta atgaatctag ctcgctagga ttgaatagct ttttaccacc gtgattttca 119640 attaagtcaa taacggtttg cagtgtctta ctagaaatac ggtcattttg acgccaacca 119700 aaacgcacgt taattagttt agctactttt tgctgtagct cactttttgc gttacctgct 119760 tctaatagtg aacctagcgc aatgatttgt gtttgatttg acatggtttt atctccaatt 119820 atgggttaaa tttacagtgt aaactgtcca tcaatttaca ctgtaaatta gggctgattt 119880 acagccctaa taattagggg ttaaatgtcc aaaccacggg cattctcgaa tgcttcacaa 119940 accttttcta aatctggttc gtaatctttc gcacttgctg ccataacgtc tgtaataccg 120000 attgcagtcg aaaccgcttc aagggctgac attttggcat ttttgaggct gtctaggtcg 120060 tcagcgtcta aacctaacgt gtgagctaga gcacgaacat agtgttcttt acgcactgtg 120120 ttagttttcg caacacttgc aactgcgtcc ggtttttggt agatacccat aactgttagt 120180 tttgagcgaa ccgctttacc tgacacggct tgaacttcct cgtgtgctgc aattgtgtct 120240 agaaaatcgt tgtgtgctgc tgcttcttta ccgtctgctt ctagtttagc attgtaaagt 120300 tctgctgctt ttgctgctga ttcttctgtc catttcttag ccatgatata ttcctcttaa 120360 taggttagtt aaatgagagt gtttctcaaa taaggcactc cgttaaaaat gccttatcgg 120420 ttaacactca aacaatccaa tcaagtaagc aataggtcaa atcgcatatt ccaaattgtt 120480 aaagagcgtg ttcccttggg aacgttagta gtatctcaaa agccgttagg cttgtcaact 120540 acttttt 120547

Claims (13)

Having a specific killing ability to bacteria Vibrio, deposited with accession number KCTC18823P, isolated bacteriophage VPT02.
The isolated bacteriophage VPT02 of claim 1, wherein the bacteriophage comprises SEQ ID NO: 1.
According to claim 1, wherein the Vibrio bacteria is enteritis Vibrio bacteria (Vibrio parahaemolyticus), Vibrio harveyi (Vibrio harveyi), or Vibrio alginolyticus (Vibrio alginolyticus) will, the isolated bacteriophage VPT02.
A composition for preventing or treating diseases caused by Vibrio, comprising the bacteriophage VPT02 of claim 1 as an active ingredient.
The composition for preventing or treating according to claim 4, wherein the disease caused by Vibrio bacteria is Vibrio disease, acute hepatopancreatic necrosis, food poisoning, necrotizing fasciitis.
A composition for inhibiting or killing Vibrio bacteria, comprising the bacteriophage VPT02 of claim 1 as an active ingredient.
The composition for inhibiting or killing Vibrio bacteria according to claim 6, wherein the composition further comprises an antibacterial agent.
The method according to claim 7, wherein the antimicrobial agent comprises 1-(5-bromothiophene-2-sulfonyl)-1H-pyrazole {1-(5-bromothiophene-2-sulfonyl)-1H-pyrazole} as an active ingredient. The composition for inhibiting or killing the growth of Vibrio bacteria.
An antibacterial agent comprising the bacteriophage VPT02 of claim 1 as an active ingredient.
A feed additive for fish and shellfish or shrimp farming, comprising the bacteriophage VPT02 of claim 1 as an active ingredient.
A disinfectant or cleaning agent comprising the bacteriophage VPT02 of claim 1 as an active ingredient.
A method of preventing or treating a disease caused by bacteria Vibrio, comprising administering the bacteriophage VPT02 of claim 1 to a subject other than a human.
A method of lysing enteritis Vibrio (Vibrio parahaemolyticus), Vibrio harveyi (Vibrio harveyi), or Vibrio alginolyticus comprising the step of treating the bacteriophage of claim 1.

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