KR20200080486A - Novel Acinetobacter baumannii specific bacteriophage AB63 and antibacterial composition comprising the same - Google Patents
Novel Acinetobacter baumannii specific bacteriophage AB63 and antibacterial composition comprising the same Download PDFInfo
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Abstract
Description
본 발명은 아시네토박터 바우마니 균에 특이적인 사멸능을 갖는 신규 박테리오파지 AB63, 상기 박테리오파지를 포함하는 항생용 조성물, 사료 첨가용 조성물, 사료, 소독제 또는 세척제 및 상기 박테리오파지를 개체에 투여하는 단계를 포함하는 아시네토박터 바우마니 균에 의한 감염성 질병의 예방 또는 치료 방법에 관한 것이다.The present invention includes the step of administering a novel bacteriophage AB63 having a specific killing ability to Acinetobacter baumani bacteria, an antibiotic composition comprising the bacteriophage, a composition for adding feed, a feed, a disinfectant or detergent, and the bacteriophage to an individual. It relates to a method for preventing or treating an infectious disease caused by Bacillus acinetobacter.
아시네토박터 바우마니(Acinetobacter baumannii) 균은 짧고 둥근 막대 모양의 그람음성균이다. 토양이나 물 등의 자연환경과 병원환경에서 매우 보편적인 기회감염균으로 면역 시스템이 손상된 사람들에게 영향을 미치며 병원 내 감염을 일으키고, 지역 사회 감염균으로도 문제가 되고 있다. 장기간 병원에 입원한 환자의 피부나 인공호흡기, 카테터 같은 장치의 표면과의 접촉으로 병원 내 집단 감염을 유발한다. 아시네토박터 바우마니 균에 의한 감염 증상은 클렙시엘라 뉴모니애(Klebsiella pneumoniae) 또는 스트렙토코커스 뉴모니애(Streptococcus pneumoniae)를 포함한 다른 기회감염균에 의한 증상과 비슷하며 발열, 오한, 발진, 근육 및 가슴 통증, 호흡 곤란 또는 기침 등이 있고, 중환자들에게 폐렴, 패혈증, 수막염, 요로감염 등을 유발하여 임상적으로 위협이 되고 있다. 과거에는 감염증의 치료가 어렵지 않았으나, 현재 항생제 내성을 나타내는 아시네토박터 바우마니 균의 분리가 점점 늘어나고 건조한 환경에서도 1개월 이상 생존할 수 있어 병원 내 감염의 상당부분을 차지하고 있다. Acinetobacter baumannii is a short, round rod-shaped Gram-negative bacterium. It is a very common opportunistic infectious bacterium in natural and hospital environments such as soil and water, affecting people with compromised immune systems, causing infections in hospitals, and is also a problem with community infectious bacteria. Contact with the skin of a patient who has been in the hospital for a long time, or the surface of a device such as a ventilator or catheter, causes infection in the hospital. Symptoms of infection by Acinetobacter baumani are similar to those caused by other opportunistic infections, including Klebsiella pneumoniae or Streptococcus pneumoniae , with fever, chills, rash, muscle and There are chest pain, shortness of breath, or cough, and it is a clinically threatening disease by causing pneumonia, sepsis, meningitis, and urinary tract infection in critically ill patients. In the past, treatment of infectious diseases was not difficult, but the separation of Acinetobacter baumani bacteria, which presently exhibit antibiotic resistance, is increasing and can survive in a dry environment for more than a month, accounting for a large portion of infection in hospitals.
세균 감염증 치료에 항생제가 널리 사용되고 있고 이에 따라 다양한 항생제에 내성을 나타내는 내성세균이 출현하고 있다. 항생제 내성이 특히 문제가 되고 있는 주요 병원균으로는 반코마이신 내성 엔테로코커스(Vancomycin-resistant Enterococci, VRE), 메티실린 내성 스타필로코커스(Methicillin-resistant Staphylococcus aureus, MRSA), Extended spectrum ß-lactamase(ESBL) 생성 그람 음성균, 카바페넴 내성 아시네토박터 바우마니(Carbapenem-resistant Acinetobacter baumannii, CRAB), 카바페넴 내성 슈도모나스 애루지노사(Carbapenem-resistant Pseudomonas aeruginosa)가 알려져 있다. 우리나라의 일부 항생제 내성균의 비율은 세계적으로 높은 수준이다. 이들의 전파를 방지하기 위해서는 철저한 병원 감염 관리가 필요하며 항생제의 사용을 더욱 신중히 해야 할 것이다. 카바페넴(Carbapenem)은 세포막 투과도가 높고 ß-lactamase 대부분에 안정하기 때문에 아시네토박터 바우마니 균 감염으로 인한 치료에는 카바페넴계 항생제인 이미페넴(imipenem) 또는 메로페넴(meropenem)을 사용하고 있다. 그러나 카바페넴계 항생제를 포함한 여러 항생제에 내성을 가진 아시네토박터 바우마니가 지속적으로 증가하고 있어 치료제로 선택할 수 있는 항생제가 제한적이다. 새로운 항생제를 개발하는 데에는 많은 비용과 시간이 소요되고 있어 항생제를 대체할 수 있는 물질의 요구가 큰 실정이다.Antibiotics are widely used to treat bacterial infections, and accordingly, resistant bacteria that are resistant to various antibiotics are emerging. The main pathogens where antibiotic resistance is of particular concern are Vancomycin-resistant Enterococci (VRE) , Methicillin-resistant Staphylococcus aureus (MRSA) , and Extended spectrum ß-lactamase (ESBL) Gram-negative bacteria , Carbapenem-resistant Acinetobacter baumannii (CRAB) , and Carbapenem-resistant Pseudomonas aeruginosa are known. The proportion of antibiotic-resistant bacteria in Korea is high worldwide. In order to prevent the spread of them, thorough hospital infection management is necessary and the use of antibiotics will be more carefully used. Carbapenem has high cell membrane permeability and is stable to most of ß-lactamase. For the treatment of Acinetobacter baumani infection, the carbapenem antibiotics imipenem or meropenem are used. However, since antibiotics that are resistant to various antibiotics, including carbapenem antibiotics, continue to increase, antibiotics that can be selected as therapeutic agents are limited. The development of new antibiotics is expensive and time-consuming, so there is a great demand for a substance that can replace antibiotics.
한편, 박테리오파지(bacteriophage)는 특정 세균을 감염시켜 감염된 세균의 성장을 억제하고 저해하는 세균 특이적 바이러스를 의미한다. 박테리오파지는 박테리아에 감염(infection)한 후 박테리아 세포 내부에서 증식을 하고, 증식 후 자손 박테리오파지들이 박테리아 밖으로 나올 때 숙주인 박테리아의 세포벽을 파괴하는 방식으로 박테리아를 사멸시키는 능력을 갖고 있다. 박테리오파지의 박테리아 감염 방식은 매우 특이성이 높아서 특정 박테리아에 감염할 수 있는 박테리오파지의 종류는 일부로 한정된다. 즉, 특정 박테리오파지는 특정 범주의 박테리아에만 감염할 수 있고 이로 인하여 특정 박테리오파지는 특정 박테리아만을 사멸시키며 다른 박테리아에는 영향을 주지 않는다. 따라서 최근 세균성 질환의 대처 방안으로 박테리오파지의 활용이 크게 주목을 받고 있다. 자연 친화적 방식의 선호로 인하여 박테리오파지에 대한 관심은 어느 때보다 높다고 할 수 있다. 기존 항생제의 대체 물질로의 개발 가능성이 부각되면서 다시 박테리오파지가 항-박테리아제로 주목을 받고 있다.On the other hand, bacteriophage (bacteriophage) refers to a bacteria-specific virus that inhibits and inhibits the growth of infected bacteria by infecting certain bacteria. The bacteriophage has the ability to kill bacteria by destroying the cell wall of the host bacteria when the progeny bacteriophages come out of the bacteria after proliferation inside the bacterial cells after infection. The method of bacterial infection of bacteriophage is very specific, so the type of bacteriophage capable of infecting certain bacteria is limited to a part. That is, a specific bacteriophage can infect only a specific category of bacteria, thereby killing a specific bacteriophage and not affecting other bacteria. Therefore, in recent years, the use of bacteriophage as a countermeasure against bacterial diseases has attracted much attention. Due to the preference of the nature-friendly method, interest in bacteriophage can be said to be higher than ever. Bacteria phage is attracting attention as an anti-bacterial agent again, as the possibility of development of alternative antibiotics as an alternative material emerges.
이에 본 발명자들은 아시네토박터 바우마니(Acinetobacter baumannii) 균에 특이적인 용균활성을 갖는 박테리오파지를 분리하고 분리된 박테리오파지의 형태적 및 유전적 특성을 분석하였으며, 상기 박테리오파지의 아시네토박터 바우마니 균, 특히 이미페넴 내성 아시네토박터 바우마니 균에 대한 탁월한 사멸 능력을 확인하였고, 상기 박테리오파지의 pH 안정성과 열 안정성을 검증함으로써 본 발명을 완성하였다.Accordingly, the present inventors isolated bacteriophage having a lytic activity specific for Acinetobacter baumannii and analyzed the morphological and genetic characteristics of the isolated bacteriophage, in particular, the Acinetobacter baumannii bacteria of the bacteriophage, in particular The excellent killing ability against imipenem-resistant Acinetobacter baumani was confirmed, and the present invention was completed by verifying the pH stability and thermal stability of the bacteriophage.
본 발명의 목적은 아시네토박터 바우마니 균에 특이적인 사멸능을 갖는, 서열번호 1로 표시되는 염기서열로 이루어진 박테리오파지 AB63을 제공하는 것이다.An object of the present invention is to provide a bacteriophage AB63 consisting of a nucleotide sequence represented by SEQ ID NO: 1, which has a specific killing ability against Acinetobacter baumani bacteria.
본 발명의 또 다른 목적은 상기 박테리오파지 AB63을 포함하는, 항생용 조성물, 사료 첨가용 조성물, 사료, 소독제 또는 세척제를 제공하는 것이다.Another object of the present invention is to provide a composition for antibiotics, a composition for adding feed, a feed, a disinfectant, or a detergent containing the bacteriophage AB63.
본 발명의 또 다른 목적은 상기 박테리오파지 AB63을 개체에 투여하는 단계를 포함하는, 아시네토박터 바우마니 균에 의한 감염성 질병의 예방 또는 치료 방법을 제공하는 것이다.Another object of the present invention is to provide a method for preventing or treating infectious diseases caused by Acinetobacter baumani bacteria, comprising administering the bacteriophage AB63 to an individual.
상기 목적을 달성하기 위하여, 본 발명은 아시네토박터 바우마니 균에 특이적인 사멸능을 갖는, 서열번호 1로 표시되는 염기서열로 이루어진 박테리오파지 AB63을 제공한다.In order to achieve the above object, the present invention provides a bacteriophage AB63 consisting of a nucleotide sequence represented by SEQ ID NO: 1, which has a specific killing ability against Acinetobacter baumani bacteria.
또한, 본 발명은 상기 박테리오파지 AB63을 포함하는, 항생용 조성물을 제공한다.In addition, the present invention provides a composition for antibiotics, comprising the bacteriophage AB63.
또한, 본 발명은 상기 박테리오파지 AB63을 포함하는, 사료 첨가용 조성물을 제공한다.In addition, the present invention provides a composition for adding feed, comprising the bacteriophage AB63.
또한, 본 발명은 상기 사료 첨가용 조성물을 포함하는 사료를 제공한다.In addition, the present invention provides a feed comprising the composition for adding the feed.
또한, 본 발명은 상기 박테리오파지 AB63을 포함하는, 소독제를 제공한다.In addition, the present invention provides a disinfectant comprising the bacteriophage AB63.
또한, 본 발명은 상기 박테리오파지 AB63을 포함하는, 세척제를 제공한다.In addition, the present invention provides a cleaning agent comprising the bacteriophage AB63.
또한, 본 발명은 상기 박테리오파지 AB63을 개체에 투여하는 단계를 포함하는, 아시네토박터 바우마니 균에 의한 감염성 질환의 예방 또는 치료 방법을 제공한다.In addition, the present invention provides a method for preventing or treating infectious diseases caused by Acinetobacter baumannii, comprising administering the bacteriophage AB63 to an individual.
본 발명의 박테리오파지 AB63은 기존의 항생제 등의 화학물질에 비해 아시네토박터 바우마니 균에 대한 특이성이 매우 높고, 카바페넴 계열의 항생제, 특히 이미페넴 내성 아시네토박터 바우마니 균에 대해서도 사멸능을 입증하여, 항생제 내성균 문제를 해소하였다. 또한 본 발명의 박테리오파지 AB63은 물리화학적 자극에 강하다는 장점을 가지고 있으며, 사람, 동물, 식물 등 세균 외의 다른 숙주에는 감염되지 않으므로, 항생제의 오남용으로 인한 항생제 내성균의 문제점, 식품 내의 항생제의 잔류 문제, 광범위한 숙주범위의 문제점을 해결할 수 있는 장점이 있다. 따라서, 본 발명의 박테리오파지 AB63은 아시네토박터 바우마니 균에 의한 감염성 질병의 예방 또는 치료, 항생용 조성물, 사료 첨가용 조성물, 사료, 소독제, 또는 세척제 분야에서 다양하게 사용될 수 있다.The bacteriophage AB63 of the present invention has a very high specificity to Acinetobacter baumani bacteria compared to chemicals such as conventional antibiotics, and demonstrates killing ability against carbapenem-based antibiotics, particularly, Imipenem-resistant Acinetobacter baumani bacteria , The antibiotic-resistant bacteria were solved. In addition, the bacteriophage AB63 of the present invention has the advantage of being strong against physicochemical stimulation, and does not infect other hosts other than bacteria, such as humans, animals, plants, and thus, problems of antibiotic-resistant bacteria due to misuse of antibiotics, problems of residual antibiotics in food, It has the advantage of solving a wide range of host problems. Therefore, the bacteriophage AB63 of the present invention can be variously used in the field of prevention or treatment of infectious diseases caused by Acinetobacter baumani, antibiotic composition, composition for adding feed, feed, disinfectant, or detergent.
도 1은 박테리오파지 AB63의 아시네토박터 바우마니 균에 대한 용균반 형성 활성을 확인한 결과를 나타낸 도이다.
도 2는 박테리오파지 AB63의 전체 유전자를 분석한 결과인 유전자 맵을 나타낸 도이다.
도 3은 전자 현미경을 통해 박테리오파지 AB63의 형태학적 특성을 확인한 결과를 나타낸 도이다.
도 4는 박테리오파지 AB63의 단백질 패턴을 SDS-PAGE를 통해 확인한 결과를 나타낸 도이다.
도 5는 박테리오파지 AB63의 아시네토박터 바우마니 균에 대한 사멸능을 확인한 결과를 나타낸 도이다.
도 6은 박테리오파지 AB63의 pH 안정성 실험 결과를 나타낸 도이다.
도 7은 박테리오파지 AB63의 온도 안정성 실험 결과를 나타낸 도이다.1 is a view showing the results of confirming the activity of the bacteriophage AB63 lysine bacterium formation activity against the Acinetobacter baumani bacteria.
2 is a diagram showing a gene map as a result of analyzing the entire gene of bacteriophage AB63.
3 is a view showing the results of confirming the morphological characteristics of bacteriophage AB63 through an electron microscope.
4 is a view showing the results of confirming the protein pattern of bacteriophage AB63 through SDS-PAGE.
5 is a view showing the results of confirming the killing ability of the bacteriophage AB63 against Acinetobacter baumani bacteria.
6 is a diagram showing the pH stability test results of bacteriophage AB63.
7 is a view showing the results of the temperature stability experiment of bacteriophage AB63.
이하, 본 발명에 대해 상세히 설명한다.Hereinafter, the present invention will be described in detail.
본 발명은 아시네토박터 바우마니 균에 특이적인 사멸능을 갖는, 서열번호 1로 표시되는 염기서열로 이루어진 박테리오파지 AB63을 제공한다.The present invention provides a bacteriophage AB63 consisting of a nucleotide sequence represented by SEQ ID NO: 1, which has a specific killing ability against Acinetobacter baumani bacteria.
본 발명에서, 용어 박테리오파지(bacteriophage)는 특정 세균에 감염하여 당해 세균의 성장을 억제하고 저해하는 세균 특이적 바이러스로, 단일 혹은 이중 사슬의 DNA 또는 RNA를 유전 물질로 포함하는 바이러스를 의미한다.In the present invention, the term bacteriophage (bacteriophage) is a bacterial specific virus that inhibits and inhibits the growth of a specific bacteria by infecting a specific bacterium, and refers to a virus comprising single or double chain DNA or RNA as a genetic material.
본 발명의 박테리오파지 AB63은 아시네토박터 바우마니 균에 대해 용균활성이 탁월하다. 상기 박테리오파지 AB63은 190 nm 내지 210 nm의 크기를 가지는 정이십면체의 머리와 수축성의 꼬리가 있는 형태인 마이오비리대(Myoviridae)에 속하며 머리의 크기는 80 nm 내지 90 nm이며, 꼬리의 길이는 110 nm 내지 120 nm로 확인되었다. The bacteriophage AB63 of the present invention has excellent lytic activity against Acinetobacter baumani bacteria. The bacteriophage AB63 belongs to Myoviridae in the form of a icosahedron head having a size of 190 nm to 210 nm and a contractile tail, and the head size is 80 nm to 90 nm, and the length of the tail is 110 nm to 120 nm.
본 발명의 박테리오파지 AB63은 단백질 패턴 분석 결과 87 kDa, 60 kDa, 50 kDa, 37 kDa, 27 kDa 그리고 20 kDa 크기의 주요 단백질을 포함함을 확인하였다. 그 전체 염기서열을 서열번호 1로 표시하였다.Bacteriophage AB63 of the present invention was confirmed that the protein pattern analysis results include 87 kDa, 60 kDa, 50 kDa, 37 kDa, 27 kDa and 20 kDa major proteins. The entire nucleotide sequence was represented by SEQ ID NO: 1.
박테리오파지 AB63은 서열번호 1로 표시되는 염기서열을 전체 유전자의 전체 또는 일부로서 포함할 수 있다. 또한, 본 발명의 박테리오파지 AB63은 서열번호 1로 표시되는 염기서열, 및 상기 염기서열의 기능적 동등물로 이루어질 수 있다. 상기 기능적 동등물이란 염기서열의 변형, 치환의 결과, 상기 서열번호 1로 표시되는 염기서열과 적어도 70% 이상, 바람직하게는 80% 이상, 더욱 바람직하게는 90% 이상, 더더욱 바람직하게는 95% 이상의 서열 상동성을 갖는 것으로, 서열번호 1로 표시되는 염기서열과 실질적으로 동질의 생리활성을 나타내는 서열을 의미한다.The bacteriophage AB63 may include the nucleotide sequence represented by SEQ ID NO: 1 as all or part of the whole gene. In addition, the bacteriophage AB63 of the present invention may be made of a base sequence represented by SEQ ID NO: 1, and a functional equivalent of the base sequence. As a result of modification and substitution of the base sequence, the functional equivalent is at least 70% or more, preferably 80% or more, more preferably 90% or more, and even more preferably 95% with the base sequence represented by SEQ ID NO:1. As having the above sequence homology, it means a sequence showing substantially the same physiological activity as the base sequence represented by SEQ ID NO: 1.
상기와 같은 아시네토박터 바우마니 균 특이적 용균활성 또는 아시네토박터 바우마니 균에 특이적인 사멸능은 본 발명의 박테리오파지 AB63을 아시네토박터 바우마니 균에 의한 감염성 질병의 예방 및 치료용 조성물, 및 상기 박테리오파지 AB63을 유효 성분으로 포함하는 다양한 제품에 적용을 가능하게 한다.The specific lytic activity of Acinetobacter baumani bacteria as described above or the killing ability specific to Acinetobacter baumani bacteria is a composition for the prevention and treatment of infectious diseases caused by the bacteriophage AB63 of the present invention by Acinetobacter baumani bacteria, and The bacteriophage AB63 makes it possible to apply to various products containing the active ingredient.
본 발명의 박테리오파지 AB63은 상기 박테리오파지가 존재할 것으로 예상되는 충북 지역의 하수처리장에서 시료를 채취하여 분리한 박테리오파지로서, 본 발명자는 이를 박테리오파지 AB63으로 명명하고 2018년 08월 14일에 한국미생물보존센터에 수탁번호 KFCC11781P로 기탁하였다. The bacteriophage of the present invention AB63 is a bacteriophage obtained by collecting and separating samples from a sewage treatment plant in the Chungbuk region where the bacteriophage is expected to exist, and the inventor named it bacteriophage AB63 and deposited it with the Korea Microbial Conservation Center on August 14, 2018. It was deposited under the number KFCC11781P.
본 발명에서 pH 안정성은 다양한 pH 범위에서 박테리오파지 AB63의 역가가 안정적으로 유지되는 것을 의미하며, 본 발명의 박테리오파지 AB63은 산성 내지 염기성의 넓은 pH범위에서 장기간 동안 안정한 것을 특징으로 할 수 있으며, 바람직하게는 pH3 내지 pH11범위에서, 더욱 바람직하게는 pH4 내지 pH11범위에서 안정한 것을 특징으로 할 수 있다.In the present invention, pH stability means that the titer of bacteriophage AB63 is stably maintained at various pH ranges, and the bacteriophage AB63 of the present invention can be characterized by being stable for a long period of time in a wide pH range of acidic or basic, preferably It can be characterized in that it is stable in the range of pH3 to pH11, more preferably in the range of pH4 to pH11.
본 발명에서 온도 안정성은 다양한 온도 범위에서 박테리오파지 AB63의 역가가 안정적으로 유지되는 것을 의미하며, 본 발명의 박테리오파지 AB63은 넓은 온도 범위에서 역가가 장기간 동안 안정한 것을 특징으로 할 수 있으며, 바람직하게는 40℃ 내지 60℃범위에서 안정한 것을 특징으로 할 수 있다.In the present invention, the temperature stability means that the titer of the bacteriophage AB63 is stably maintained in various temperature ranges, and the bacteriophage AB63 of the present invention can be characterized in that the titer is stable for a long time in a wide temperature range, preferably 40°C. It can be characterized in that it is stable in the range of to 60 ℃.
본 발명에서 아시네토박터 바우마니 균 특이적 사멸능은 박테리오파지 AB63이 아시네토박터 바우마니 균 이외의 다른 균을 사멸시키지 않으면서, 특이적으로 아시네토박터 바우마니 균만 사멸시키는 능력을 의미하며, 특히 카바페넴 계열 항생제에 내성을 가지는 아시네토박터 바우마니 균을 특이적으로 사멸시키는 것을 특징으로 한다. 상기 카바페넴 계열 항생제는 이미페넴, 메로페넴, 에르타페넴 및 파로페넴으로 이루어진 군에서 선택된 어느 하나일 수 있으며, 바람직하게는 이미페넴인 것을 특징으로 할 수 있다. In the present invention, the specific killing ability of Acinetobacter baumani bacteria means the ability to specifically kill only Acinetobacter baumani bacteria, while bacteriophage AB63 does not kill any other bacteria other than Acinetobacter baumani bacteria, in particular It is characterized by specifically killing Acinetobacter baumani bacteria resistant to carbapenem antibiotics. The carbapenem-based antibiotic may be any one selected from the group consisting of imipenem, meropenem, ertapenem, and paropenem, and may be characterized as being imipenem.
또한, 본 발명은 상기 박테리오파지 AB63을 포함하는, 항생용 조성물을 제공한다. In addition, the present invention provides a composition for antibiotics, comprising the bacteriophage AB63.
본 발명에서, 용어 항생용 조성물은 약제 형태로 동물에게 제공되어 균을 사멸시킬 수 있는 제제를 의미하며, 방부제, 살균제, 항생제 및 항균제를 총칭하는 것이다. In the present invention, the term antibiotic composition refers to an agent that can be provided to animals in the form of a drug to kill bacteria, and is a generic term for preservatives, fungicides, antibiotics, and antibacterial agents.
본 발명의 박테리오파지 AB63은 기존 항생제에 비하여 아시네토박터 바우마니 균에 대한 특이성이 매우 높고, 유익균은 죽이지 않으면서 아시네토박터 바우마니 균만 사멸시킬 수 있다. 아울러, 약물 내성 내지 저항성을 유도하지 않아, 기존의 항생물질에 비하여 제품수명(life cycling)이 긴 신규 항생제로서 이용될 수 있다는 이점이 있다.The bacteriophage AB63 of the present invention has a very high specificity to the Acinetobacter baumani bacteria compared to conventional antibiotics, and can kill only the Acinetobacter baumani bacteria without killing beneficial bacteria. In addition, since it does not induce drug resistance or resistance, there is an advantage that it can be used as a new antibiotic with a long product life (life cycling) compared to an existing antibiotic.
또한, 본 발명은 상기 박테리오파지 AB63을 포함하는, 사료 첨가용 조성물을 제공한다.In addition, the present invention provides a composition for adding feed, comprising the bacteriophage AB63.
축산, 수산업에서 사용되는 사료 첨가용 항생제는 질병의 예방 목적으로 사용되고 있는데, 예방 목적의 항생제 투여는 내성균 발생 가능성을 높이고 가축에 잔류하는 항생제가 사람에게 전달될 수 있어서 문제이다. 항생제가 육류를 통해 인체에 흡수되면 항생제 내성을 유발해 질병의 확산을 부를 수도 있다. 또한, 사료에 섞어 먹이는 항생제의 종류가 많고 이는 다제 내성균 발생 확률이 높아지는 문제점이 있기 때문에 좀 더 자연 친화적이면서도 기존의 항생제의 사용에서 발생한 문제를 해결할 새로운 사료 첨가용 항생물질로서 본 발명의 상기 박테리오파지 AB63을 이용할 수 있다.Antibiotics for feed addition used in animal husbandry and fisheries are used for the purpose of preventing diseases, but the administration of antibiotics for prevention increases the possibility of developing resistant bacteria and antibiotics remaining in livestock can be delivered to humans. When antibiotics are absorbed into the human body through meat, they can cause antibiotic resistance and cause disease spread. In addition, there are many types of antibiotics mixed with feed, which is more natural-friendly and has a problem of increasing the probability of multi-drug-resistant bacteria, but as a new feed additive antibiotic to solve the problems arising from the use of existing antibiotics, the bacteriophage of the present invention AB63 Can be used.
또한, 본 발명은 상기 사료 첨가용 조성물을 포함하는 사료를 제공할 수 있으며, 본 발명의 사료는 박테리오파지를 사료 첨가제 형태로 따로 제조하여 사료에 혼합시키거나, 사료 제조 시 직접 첨가시켜 제조할 수 있다. 본 발명의 사료 내 박테리오파지는 액상 또는 건조상태일 수 있으며, 바람직하게는 건조된 분말형태이다. 건조방법은 통풍건조, 자연건조, 분무건조 및 동결건조가 가능하지만, 이에 제한되는 것은 아니다. 본 발명의 박테리오파지는 분말형태로 사료 중량의 0.05 중량% 내지 10 중량%, 바람직하게는 0.1 중량% 내지 2 중량%의 성분비로 혼합될 수 있다. 또한, 상기 사료는 본 발명의 박테리오파지 외에 사료의 보존성을 높일 수 있는 통상의 첨가제들을 추가로 포함할 수 있다.In addition, the present invention can provide a feed containing the composition for adding the feed, and the feed of the present invention can be prepared by separately preparing bacteriophage in the form of a feed additive and mixing it with feed, or by directly adding it when preparing the feed. . The bacteriophage in the feed of the present invention may be in a liquid or dry state, preferably in the form of a dried powder. The drying method may include ventilation drying, natural drying, spray drying, and freeze drying, but is not limited thereto. The bacteriophage of the present invention may be mixed in a powder form in an ingredient ratio of 0.05% to 10% by weight, preferably 0.1% to 2% by weight of the feed weight. In addition, the feed may further include conventional additives that can increase the preservability of the feed in addition to the bacteriophage of the present invention.
본 발명의 사료 첨가용 조성물에는 비병원성의 다른 미생물이 추가로 첨가될 수 있다. 첨가될 수 있는 미생물로는 단백질 분해 효소, 지질 분해효소 및 당 전환 효소를 생산할 수 있는 바실러스 서브틸리스(Bacillus subtilis)와 같은 고초균, 소의 위와 같은 혐기적 조건에서 생리적 활성 및 유기물 분해능이 있는 락토바실러스 균주(Lactobacillus sp.), 가축의 체중을 증가시키며 우유의 산유량을 늘리고 사료의 소화 흡수율을 높이는 효과를 보여주는 아스퍼질러스 오리자에(Aspergillus oryzae)와 같은 사상균 및 사카로미세스 세레비지에(Saccharomyces cerevisiae)와 같은 효모로 구성된 군으로부터 선택될 수 있다.In the composition for adding feed of the present invention, other non-pathogenic microorganisms may be further added. Microorganisms that can be added include proteases such as Bacillus subtilis , which can produce proteolytic enzymes, lipid-degrading enzymes, and sugar-converting enzymes, and Lactobacillus with physiological activity and organic matter-decomposing ability in anaerobic conditions such as stomach. Strains ( Lactobacillus sp.), filamentous fungi such as Aspergillus oryzae and Saccharomyces cerevisiae, which show the effect of increasing the weight of livestock, increasing the amount of milk produced and increasing the digestion and absorption rate of feed. ) May be selected from the group consisting of yeast.
본 발명의 박테리오파지 AB63을 포함하는 사료에는 식물성으로는 곡물류, 근괴류, 식품가공 부산물류, 조류, 섬유질류, 제약 부산물류, 유지류, 전분류, 박류, 곡물부산물류 등이 있으며, 동물성으로는 단백질류, 무기물류, 유지류, 광물성류, 단세포 단백질, 동물성 플랑크톤류, 남은 음식물 등이 있으며 이에 제한되는 것은 아니다.The feed containing the bacteriophage AB63 of the present invention includes grains, root masses, food processing by-products, algae, fiber, pharmaceutical by-products, fats and oils, starch, peels, grain by-products, etc. Species, minerals, oils, minerals, unicellular proteins, animal plankton, leftovers, and the like, but are not limited thereto.
본 발명의 사료 첨가용 조성물에는 품질 저하를 방지하기 위하여 첨가하는 결착제, 유화제, 보존제 등이 포함될 수 있고, 효용 증대를 위하여 사료에 첨가하는 아미노산제, 비타민제, 효소제, 생균제, 향미제, 비단백태질소화합물, 규산염제, 완충제, 착색제, 추출제, 올리고당 등이 있으며, 그 외에도 사료 혼합제 등을 추가로 포함할 수 있다.The composition for feed addition of the present invention may include a binder, an emulsifier, a preservative, etc., added to prevent quality degradation, and an amino acid agent, vitamin agent, enzyme agent, probiotic agent, flavoring agent, non-proteinaceous agent added to the feed for increased utility Nitrogen compounds, silicates, buffers, colorants, extractants, oligosaccharides, etc. In addition, it may further include a feed mixture.
또한, 본 발명은 박테리오파지 AB63을 포함하는, 음용수 첨가제를 제공한다.In addition, the present invention provides a drinking water additive, including bacteriophage AB63.
본 발명의 음용수 첨가제는 상기 박테리오파지 AB63 또는 이를 포함하는 조성물을 음용수 첨가제 형태로 따로 제조하여 사료 또는 음용수에 혼합하는 방식으로 사용되거나, 음용수 제조 시 직접 첨가하는 방식으로 사용할 수 있다. 상기와 같이 음용수에 혼합하여 공급함으로써 지속적으로 아시네토박터 바우마니 균의 숫자를 감소시킬 수 있는 효과가 있다.Drinking water additives of the present invention can be used as a method of separately preparing the bacteriophage AB63 or a composition containing the same in the form of drinking water additives to be mixed with feed or drinking water, or can be used as a method of adding directly when preparing drinking water. As described above, there is an effect of continuously reducing the number of Acinetobacter baumani bacteria by mixing and supplying it with drinking water.
본 발명에서 음용수는 특별히 제한되지 아니하며, 당해 기술 분야에서 통상적으로 사용되는 음용수를 사용할 수 있다.In the present invention, drinking water is not particularly limited, and drinking water commonly used in the art may be used.
또한, 본 발명은 상기 박테리오파지 AB63을 포함하는, 소독제를 제공한다.In addition, the present invention provides a disinfectant comprising the bacteriophage AB63.
아시네토박터 바우마니 균에 특이적 사멸능을 갖는 본 발명의 박테리오파지 AB63을 포함하는 소독제는 병원감염을 막기 위한 병원 및 보건용의 소독제로 유용하게 사용될 수 있고 일반 생활 소독제, 식품 및 조리 장소 및 설비의 소독제, 양계장, 축사 등의 건물, 축체, 음수, 깔짚, 난좌, 운반차량, 식기 등의 각종 생육 용품의 소독 등에 사용될 수 있다.Disinfectants containing bacteriophage AB63 of the present invention having a specific killing ability against Acinetobacter baumani bacteria can be usefully used as disinfectants for hospitals and health to prevent hospital infections, and general life disinfectants, food and cooking places and facilities It can be used for disinfection of various growth products, such as disinfectants, poultry farms, houses, buildings, livestock, drinking water, litter, seats, transport vehicles, tableware.
또한, 본 발명은 상기 박테리오파지 AB63을 포함하는, 세척제를 제공한다.In addition, the present invention provides a cleaning agent comprising the bacteriophage AB63.
본 발명의 박테리오파지 AB63은 아시네토박터 바우마니 균에 특이적 사멸능을 가지므로, 아시네토박터 바우마니 균에 노출되었거나 노출될 가능성이 있는 개체의 피부 표면 또는 신체 각 부위 등을 세척하는 용도로도 사용될 수 있다.Since the bacteriophage AB63 of the present invention has a specific killing ability against Acinetobacter baumani, it is also used for washing the skin surface or parts of the body of an individual who is or is likely to be exposed to Acinetobacter baumani. Can be used.
또한, 본 발명은 상기 박테리오파지 AB63을 개체에 투여하는 단계를 포함하는 아시네토박터 바우마니 균에 의한 감염성 질병의 예방 또는 치료 방법을 제공한다.In addition, the present invention provides a method for preventing or treating infectious diseases caused by Acinetobacter baumani bacteria, comprising administering the bacteriophage AB63 to an individual.
본 발명에서 아시네토박터 바우마니 균에 의한 감염성 질병은 아시네토박터 바우마니 감염성 폐렴, 아시네토박터 바우마니 감염성 패혈증, 아시네토박터 바우마니 감염성 수막염 및 아시네토박터 바우마니 감염성 요로감염으로 이루어진 군에서 선택된 1개 이상의 질병이며, 발열, 오한, 발진, 근육 및 가슴 통증, 호흡 곤란 또는 기침 등의 증상을 유발할 수 있으나, 이에 제한되는 것은 아니다.In the present invention, the infectious disease caused by Acinetobacter baumani bacteria is from the group consisting of Acinetobacter baumani infectious pneumonia, Acinetobacter baumani infectious sepsis, Acinetobacter baumani infectious meningitis and Acinetobacter baumani infectious urinary tract infection. It is one or more selected diseases, and may cause symptoms such as fever, chills, rash, muscle and chest pain, difficulty breathing, or coughing, but is not limited thereto.
아시네토박터 바우마니 균에 의한 감염성 질병은 통상 카바페넴계 항생제, 특히 이미페넴으로 치료하고 있다.Infectious diseases caused by Acinetobacter baumani bacteria are usually treated with carbapenem antibiotics, especially imipenem.
이미페넴은 세포막 투과도가 높고, β-lactamase에 안정하므로 아시네토박터 바우마니 균에 의한 감염성 질병을 치료하는 주요 항생제로 사용되고 있다. 다만, 카바페넴계 항생제에 내성을 나타내는 아시네토박터 바우마니 균이 기존의 항생요법으로는 사멸이 되지 않아 문제가 되나, 본 발명의 박테리오파지 AB63은 카바페넴계 항생제 내성 아시네토박터 바우마니 균 사멸능이 있음을 입증한 바, 카바페넴계 항생제에 내성을 지니는 아시네토박터 바우마니 균을 사멸시키는 박테리오파지 AB63을 제공한다. Imipenem has high cell membrane permeability and is stable to β-lactamase, so it is used as a major antibiotic to treat infectious diseases caused by Acinetobacter baumani. However, the bacteriophage AB63 of the present invention has a bacteriophages AB63 that is resistant to carbapenem-based antibiotics, but the bacteriophage AB63 of the present invention has the ability to kill the bacteriophage-based antibiotics. It has been proven that it provides a bacteriophage AB63 that kills Acinetobacter baumani bacteria resistant to carbapenem antibiotics.
본 발명에서 아시네토박터 바우마니 균, 특히 이미페넴 내성 아시네토박터 바우마니 균에 의한 감염성 질병은 박테리오파지 AB63에 의해 아시네토박터 바우마니균 또는 이미페넴 내성 아시네토박터 바우마니 균이 사멸됨으로써 예방 또는 치료되는 것을 특징으로 한다. In the present invention, the infectious disease caused by Acinetobacter baumani bacteria, in particular, Imipenem-resistant Acinetobacter baumani bacteria, is prevented or treated by the killing of Acinetobacter baumani bacteria or imipenem-resistant Acinetobacter baumani bacteria by bacteriophage AB63. It is characterized by.
본 발명에서, 용어 "개체"는 아시네토박터 바우마니 균에 의한 감염성 질병이 발병될 수 있는 모든 개체를 의미하는 것으로 인간을 포함한 포유동물, 가축류 또는 가금류를 포함하나, 이에 제한되는 것은 아니다. In the present invention, the term "individual" refers to any individual capable of developing an infectious disease caused by the Acinetobacter baumani bacteria, including but not limited to mammals, livestock or poultry, including humans.
상기 용어 "가축류"는 인간에 의하여 순화, 개량되어 사람과 함께 공동생활을 하는 유용한 동물을 지칭하는 개념으로, 예를 들어, 돼지, 소, 닭, 말, 오리 또는 개 등을 포함하나, 이에 제한되는 것은 아니다.The term "livestock" is a concept that refers to a useful animal that has been purified and improved by humans and has a common life with humans, including, but not limited to, pigs, cows, chickens, horses, ducks or dogs, etc. It does not work.
상기 용어 "가금류"는, 가축 중 조류에 속하는 동물을 총칭하여 이르는 개념으로, 예를 들어, 닭, 오리, 꿩, 메추리, 타조, 거위 또는 칠면조 등을 포함하나, 이에 제한되는 것은 아니다.The term "poultry" is a concept that collectively refers to animals belonging to birds among livestock, including, but not limited to, chickens, ducks, pheasants, quails, ostrich, goose or turkey, and the like.
본 발명의 방법에서 상기 박테리오파지 AB63 또는 조성물은 약학적 제제의 형태로 동물에게 투여되거나, 가축의 사료 또는 음용수에 혼합하여 이를 섭식시키는 방법을 통해 투여될 수 있다.In the method of the present invention, the bacteriophage AB63 or the composition may be administered to an animal in the form of a pharmaceutical preparation, or may be administered through a method of feeding it by mixing it with feed or drinking water of a livestock.
본 발명의 방법에서 상기 박테리오파지 AB63 또는 조성물의 투여 경로는 목적 조직에 도달할 수 있는 한 경구 또는 비경구의 다양한 경로를 통하여 투여될 수 있으며, 구체적으로, 구강, 직장, 국소, 정맥 내, 복강 내, 근육 내, 동맥 내, 경피, 비측 내, 흡입 등을 통해 통상적인 방식으로 투여될 수 있다.In the method of the present invention, the route of administration of the bacteriophage AB63 or the composition can be administered through various routes of oral or parenteral as long as it can reach the target tissue, specifically, oral, rectal, topical, intravenous, intraperitoneal, Intramuscular, intraarterial, transdermal, intranasal, inhalation, and the like can be administered in a conventional manner.
본 발명의 방법에 투여되는 상기 박테리오파지 AB63의 적합한 총 1일 사용량은 올바른 의학적 판단범위 내에서 처치의에 의해 결정될 수 있다는 것은 당업자에게 자명한 일이다. 특정 개체에 대한 구체적인 치료적 유효량은 달성하고자 하는 반응의 종류와 정도, 환자의 연령, 체중, 일반 건강 상태, 성별 및 식이, 투여시간, 투여 경로 및 조성물의 분비율, 치료기간, 구체적 조성물과 함께 사용되거나 동시 사용되는 약물을 비롯한 다양한 인자와 의약 분야에 잘 알려진 유사 인자에 따라 다르게 적용하는 것이 바람직하다.It is obvious to those skilled in the art that the appropriate total daily use amount of the bacteriophage AB63 administered to the method of the present invention can be determined by the treating physician within the proper medical judgment. The specific therapeutically effective amount for a particular individual is accompanied by the type and extent of the response to be achieved, the patient's age, weight, general health status, sex and diet, time of administration, route of administration and composition secretion rate, duration of treatment, and specific composition It is desirable to apply differently according to various factors including drugs used or co-used and similar factors well known in the pharmaceutical field.
중복되는 내용은 본 명세서의 복잡성을 고려하여 생락하며, 본 명세서에서 달리 정의되지 않은 용어들은 본 발명이 속하는 기술분야에서 통상적으로 사용되는 의미를 갖는 것이다.Duplicate contents are omitted in consideration of the complexity of the present specification, and terms that are not otherwise defined herein have meanings commonly used in the technical field to which the present invention pertains.
이하, 본 발명을 실시예, 제제예에 의해 상세히 설명한다. 단, 하기 실시예, 제제예는 본 발명을 예시하는 것일 뿐 본 발명의 내용이 하기 실시예, 제제예에 한정되는 것은 아니다.Hereinafter, the present invention will be described in detail by examples and formulation examples. However, the following examples and formulation examples are only illustrative of the present invention, and the contents of the present invention are not limited to the following examples and formulation examples.
실시예 1. 신규한 박테리오파지의 분리 및 배양 Example 1. Isolation and culture of novel bacteriophage
1.1 박테리오 파지의 스크리닝 및 분리1.1 Screening and separation of bacteriophage
대상세균인 이미페넴 내성 아시네토박터 바우마니 균은 당사에서 아시아태평양 감염연구소에서 분양 받은 것을 사용하였다. 충북 지역의 하수처리장에서 채취한 시료를 대상세균과 함께 35℃에서 하룻밤 동안 진탕배양 하였다. 배양액을 4,000 rpm으로 20분간 원심분리하여 상등액을 공극 크기가 0.2 μm인 필터를 이용하여 여과한 후, 이렇게 얻어진 여과액을 이용하여 용균반 분석을 통해 아시네토박터 바우마니 균에 특이적인 박테리오파지를 선별하였다. The target bacterium, Imipenem-resistant Acinetobacter baumani, was used by the company from the Asia Pacific Infection Research Institute. Samples collected from the sewage treatment plant in Chungbuk area were cultured with the target bacteria at 35°C overnight. The culture solution was centrifuged at 4,000 rpm for 20 minutes, and the supernatant was filtered using a filter having a pore size of 0.2 μm, and then the bacteriophage specific to Acinetobacter baumannii was selected through lysis analysis using the obtained filtrate. Did.
박테리오파지를 스크리닝 하기 위해서 구체적으로 전배양된 대상세균 150 μL를 취해서 멸균된 튜브에 넣고 3 mL의 BHI 탑 아가[뇌심장침출액(뇌 침출 솔리드(solids) 12.5 g, 소 심장 침출 솔리드 5.0 g, 프로테오스펩톤 10.0 g, 글루코스 2.0 g, 소듐클로라이드 5 g, 인산나트륨 2.5 g/L) + 0.6% 아가]를 첨가하여 잘 혼합한 후 미리 만들어 둔 2% BHI 아가 플레이트에 중층하였다. 실온에서 30분간 방치한 후 준비된 샘플 여과액을 각각 10 μL씩 점적한 후 실온에서 30분간 방치 후 35℃에서 24시간 배양하여 박테리오파지의 유무를 확인하였다. 박테리오파지의 존재를 확인한 샘플로부터 파지를 순수 분리하기 위해서 소프트 아가 오버레이법을 이용하였다. 박테리오파지가 있다고 확인된 샘플을 식염수로 적절히 희석하여 각 희석단의 샘플 희석액 100 μL를 취하여 대상 세균 배양액 150 μL에 혼합한 후 0.6% BHI 탑 아가를 3 mL 첨가하여 잘 혼합한 뒤, 미리 만들어 둔 2% BHI 아가 플레이트에 중층하여 방치한 후 35℃에서 24시간 배양하였다. 배양한 각 플레이트에서 형성된 용균반을 확인한 후 선택하여 400 μL의 SM 완충액(NaCl 5.8 g/L; MgSO47H2O 2 g/L; 1M Tris-Cl(pH7.5) 50 mL)에 현탁하고, 실온에서 4시간동안 방치하였다. 이 박테리오파지 액을 100 μL를 0.6% BHI 탑 아가와 세균배양액 500 μL와 혼합한 뒤 150 mm 지름의 BHI 플레이트에 분주하여 중층한 후 완전히 용균이 일어날 때까지 35℃에서 배양하였다. 배양이 종료된 후, BHI 플레이트에 15 mL의 SM 완충액을 가하고 실온에서 4시간동안 천천히 교반하면서 박테리오파지 용액을 회수한 후 4,000 rpm으로 20분간 원심분리하여 상등액만 취하고 이를 0.2 μm 필터로 여과하여 최종 박테리오파지 용액을 회수하였다. 이렇게 얻어진 최종 박테리오파지의 용균반을 확인한 결과를 도 1에 나타내었다. To screen for bacteriophage, take 150 μL of pre-cultured target bacteria and place them in sterilized tubes. 3 mL of BHI top agar (brain heart leach (12.5 g of brain leach solids, 5.0 g of bovine heart leach solids, proteo) 10.0 g of peptone, 2.0 g of glucose, 5 g of sodium chloride, 2.5 g/L of sodium phosphate + 0.6% agar] were added and mixed well, and then layered on a 2% BHI agar plate prepared in advance. After standing at room temperature for 30 minutes, 10 μL of the prepared sample filtrate was added dropwise, and then left at room temperature for 30 minutes, and then cultured at 35° C. for 24 hours to confirm the presence or absence of bacteriophage. A soft agar overlay method was used to purely separate phages from samples confirming the presence of bacteriophage. Samples identified as having bacteriophage are appropriately diluted with saline, 100 μL of the sample dilution of each dilution stage is taken, mixed with 150 μL of the target bacterial culture, 3 mL of 0.6% BHI top agar is added, mixed well, and prepared in advance. % BHI Agar Plate was layered and left standing, followed by incubation at 35°C for 24 hours. After confirming the lysate formed on each incubated plate, select and suspend in 400 μL of SM buffer (NaCl 5.8 g/L; MgSO47H2O 2 g/L; 1M Tris-Cl (pH7.5) 50 mL), and at room temperature. It was left for an hour. The bacteriophage solution was mixed with 100 μL of 0.6% BHI top agar and 500 μL of a bacterial culture medium, and then dispensed on a 150 mm diameter BHI plate, layered, and incubated at 35° C. until complete lysis. After the incubation was completed, 15 mL of SM buffer was added to the BHI plate, and the bacteriophage solution was recovered while slowly stirring at room temperature for 4 hours, and then centrifuged at 4,000 rpm for 20 minutes to take only the supernatant and filter it with a 0.2 μm filter to obtain the final bacteriophage. The solution was recovered. The results of confirming the lysis plate of the final bacteriophage thus obtained are shown in FIG. 1.
도 1에 나타난 바와 같이, 1 mm 내지 2 mm의 크기를 가지는 용균반을 형성하는 박테리오파지를 확인하였고, 상기 박테리오파지는 아시네토박터 바우마니 균에 특이적인 용균능을 갖는 박테리오파지임을 확인하였다.As shown in Figure 1, it was confirmed that the bacteriophage forming a bacterial plaque having a size of 1 mm to 2 mm, it was confirmed that the bacteriophage is a bacteriophage having a specific solubility ability to the Acinetobacter baumani bacteria.
1.2 박테리오파지의 대량 배양 및 정제1.2 Mass culture and purification of bacteriophage
박테리오파지를 대량 배양하기 위하여, 1.1에서 회수한 최종 박테리오파지 배양액을 1.0x108 PFU/mL로 희석한 후 전배양된 세균배양액(1.0x109 CFU/mL)과 혼합하여 MOI (Multiplicity of infection)를 0.1이 되도록 하여 35℃에서 100 rpm으로 18시간 배양하였다. 배양이 종료된 후 전량의 배양액을 6,000 rpm으로 30분간 원심분리하여 상등액을 수득하였고, 이를 0.2 μm 필터로 여과하여 최종 박테리오파지 배양액을 회수하였다.In order to mass culture the bacteriophage, the final bacteriophage culture collected in 1.1 was diluted with 1.0x10 8 PFU/mL, mixed with pre-cultured bacterial culture solution (1.0x10 9 CFU/mL), and the MOI (Multiplicity of infection) was 0.1. The mixture was incubated at 35°C at 100 rpm for 18 hours. After the cultivation was completed, the whole amount of the culture solution was centrifuged at 6,000 rpm for 30 minutes to obtain a supernatant, and the final bacteriophage culture solution was collected by filtration through a 0.2 μm filter.
분리된 파지를 박테리오파지 AB63으로 명명하고, 2018년 8월 14일, 한국미생물보존센터 (Korean Culture Center of Microorganisms, 서울, 한국)에 기탁하여 기탁번호 KFCC11781P를 부여 받았다.The separated phage was named bacteriophage AB63 and deposited on the 14th of August 2018 at the Korean Culture Center of Microorganisms (Seoul, Korea) to receive a deposit number KFCC11781P.
실시예 2. 박테리오파지 AB63의 전체 유전자 분석 Example 2. Whole gene analysis of bacteriophage AB63
파지 배양액에 20 mM EDTA, 50 μg/mL의 Proteinase K, 0.5%(w/v) SDS 가한 후, 50℃에서 1시간 동안 정치하였다. 동량의 Phenol-Chloroform-Isopropyl alcohol(25:24:1)과 혼합한 후 실온에서 12,000 rpm으로 10분간 원심분리하여 상등액을 회수하였다. 회수된 상등액에 동일 부피의 Phenol-Chloroform-Isopropyl alcohol(25:24:1)과 혼합하여 실온에서 12,000 rpm으로 10분간 원심분리한 후 상등액을 회수하고, 상등액에 3M sodium acetate를 전체 부피의 10%(v/v)가 되도록 가한 후 혼합하였다. 여기에 2배 부피의 차가운 95% 에탄올을 가하여 혼합한 뒤 -20℃에서 1시간 동안 정치하였고, 반응이 끝난 후 0℃에서 10분간 12,000 rpm으로 원심분리한 후 침전물 획득하였고, 차가운 70% 에탄올로 2회 세척한 뒤 에탄올을 완전히 제거하여 DNA pellet을 회수하였고 이를 최종 50 μL TE buffer(Tris-EDTA, pH8.0)에 용해시킨 후 DNA 농도를 측정하였다. 전체 유전자 분석을 위해 마크로젠사의 PacBioRSII 를 이용하여 유전자 시퀀싱을 시행하였다. After 20 mM EDTA, 50 μg/mL Proteinase K, and 0.5% (w/v) SDS were added to the phage culture, the mixture was left standing at 50° C. for 1 hour. After mixing with the same amount of Phenol-Chloroform-Isopropyl alcohol (25:24:1), the supernatant was recovered by centrifugation at 12,000 rpm for 10 minutes at room temperature. The supernatant was mixed with the same volume of Phenol-Chloroform-Isopropyl alcohol (25:24:1) in the recovered supernatant and centrifuged at 12,000 rpm for 10 minutes at room temperature to recover the supernatant, and 3M sodium acetate in the supernatant was 10% of the total volume. (v/v) was added and mixed. After adding and mixing 2 times the volume of cold 95% ethanol, the mixture was allowed to stand at -20°C for 1 hour, and after the reaction was completed, centrifugation was performed at 0°C for 10 minutes at 12,000 rpm to obtain a precipitate, and cold 70% ethanol was used. After washing twice, the ethanol was completely removed to recover the DNA pellet, which was dissolved in the final 50 μL TE buffer (Tris-EDTA, pH8.0), and then the DNA concentration was measured. Gene sequencing was performed using Macrogen's PacBioRSII for overall gene analysis.
도 2에 나타낸 바와 같이, 유전자 시퀀싱 분석을 수행한 결과, 박테리오파지 AB63의 전체 유전자의 크기는 87,957 bp 이며, GC함량이 39.2%, 전체 ORFs의 수는 136개임을 확인하였다. 상기 분석한 박테리오파지 AB63의 전체 유전자 서열을 서열번호 1로 나타내었다.As shown in FIG. 2, as a result of performing the gene sequencing analysis, it was confirmed that the total gene size of bacteriophage AB63 was 87,957 bp, the GC content was 39.2%, and the total number of ORFs was 136. The entire gene sequence of the analyzed bacteriophage AB63 is shown in SEQ ID NO: 1.
실시예 3. 박테리오파지 AB63의 형태학적 특성 Example 3. Morphological properties of bacteriophage AB63
분리된 박테리오파지의 형태학적 특성을 알아보기 위해, 박테리오파지 AB63을 0.01% 젤라틴 용액에 희석하여 2.5% 글루타르알데하이드(glutaraldehyde) 용액으로 고정하였다. 이를 carbon-coated mica plate(ca. 2.5 x 2.5 mm)에 적가한 후, 10분간 적응시켜 멸균증류수로 세척하였다. 이를 구리 격자(copper grid)에 끼워 2% 우라닐 아세테이트(Uranyl acetate)에서 30-60초간 염색하고 건조시킨 후, 투과전자현미경(Tecnai G2 Spirit Twin, Bio-Transmission electron microscope, 120 kV, 120,000~400,000)으로 검경하여 박테리오파지 AB63의 전자현미경 사진을 도 3에 나타내었다. To investigate the morphological properties of the separated bacteriophage, bacteriophage AB63 was diluted in a 0.01% gelatin solution and fixed with a 2.5% glutaraldehyde solution. This was added dropwise to a carbon-coated mica plate (ca. 2.5 x 2.5 mm), then adapted for 10 minutes and washed with sterile distilled water. This was put on a copper grid, dyed for 30-60 seconds in 2% uranyl acetate, dried, and then transferred to a Tecnai G2 Spirit Twin, Bio-Transmission electron microscope, 120 kV, 120,000 to 400,000 3) shows an electron microscope photograph of bacteriophage AB63.
도 3에 나타난 바와 같이, 박테리오파지 AB63은 190 nm 내지 210 nm의 크기를 가지는 정이십면체의 머리와 수축성의 꼬리가 있는 형태인 마이오비리대(Myoviridae)에 속함을 알 수 있었다. 머리의 크기는 80 nm 내지 90 nm이며, 꼬리의 길이는 110 nm 내지 120 nm로 확인하였다.As shown in FIG. 3, it was found that the bacteriophage AB63 belongs to Myoviridae , which is a icosahedral head having a size of 190 nm to 210 nm and a contractile tail. The size of the head was 80 nm to 90 nm, and the length of the tail was confirmed to be 110 nm to 120 nm.
실시예 4. 박테리오파지 AB63의 단백질 패턴 분석 Example 4. Protein pattern analysis of bacteriophage AB63
1.0x1010 PFU/mL로 준비된 박테리오파지 AB63 40 μL와 10 μL의 5X loading buffer를 혼합하고, 10분간 끓인 다음 12.5% SDS-PAGE를 수행한 결과를 도 4에 나타내었다. The results of mixing 10 μL of 5X loading buffer with 10 μL of bacteriophage AB63 prepared at 1.0 × 10 10 PFU/mL, boiling for 10 minutes, and then performing 12.5% SDS-PAGE are shown in FIG. 4.
도 4에 나타난 바와 같이, 박테리오파지 AB63의 단백질을 전기영동한 결과, 87 kDa, 60 kDa, 50 kDa, 37 kDa, 27 kDa 그리고 20 kDa 크기의 주요 단백질을 확인하였다.As shown in FIG. 4, as a result of electrophoresis of the protein of bacteriophage AB63, major proteins of 87 kDa, 60 kDa, 50 kDa, 37 kDa, 27 kDa and 20 kDa were identified.
실시예 5. 박테리오파지 AB63의 아시네토박터 바우마니 균에 대한 사멸능의 확인 Example 5. Confirmation of killing ability of bacteriophage AB63 against Acinetobacter baumani bacteria
박테리오파지 AB63의 아시네토박터 바우마니 균에 대한 사멸 능력을 분석하기 위하여, 600 nm에서 흡광도가 0.35 되는 세균 배양액을 10 mL 준비하였다. 세균 배양액에 농도가 1.0x108 PFU/mL 되는 박테리오파지액을 1 mL 넣어 MOI 1로 접종하고, 1.0x107 PFU/mL 농도의 박테리오파지액을 1 mL 넣어 MOI 0.1로 접종하여 두 가지 조건으로 실험하였다. 박테리오파지액을 넣지 않은 시료를 대조군으로 하였다. 4시간 동안 배양하며 1시간 단위로 흡광도 600 nm에서 세균의 배양 상태를 관찰한 결과를 표 1과 도 5에 나타내었다. In order to analyze the killing ability of bacteriophage AB63 against Acinetobacter baumani bacteria, 10 mL of a bacterial culture solution having an absorbance of 0.35 at 600 nm was prepared. The bacteriophage solution having a concentration of 1.0x10 8 PFU/mL was inoculated with
[표 1][Table 1]
표 1과 도 5에 나타난 바와 같이, 박테리오파지 AB63을 첨가하지 않은 대조군의 경우 4시간 후 600 nm에서 흡광도가 4.68로 나타나 세균이 잘 성장한 것을 확인할 수 있다. 반면, 박테리오파지 AB63을 첨가한 실험군의 경우 4시간 경과했을 때 600 nm에서 흡광도가 0.86과 1.16으로 나타나 박테리오파지 AB63이 아시네토박터 바우마니 균의 사멸에 탁월한 효과를 나타내는 것을 확인하였다.As shown in Table 1 and Fig. 5, in the case of the control group without addition of bacteriophage AB63, the absorbance was 4.68 at 600 nm after 4 hours, indicating that the bacteria had grown well. On the other hand, in the case of the experimental group in which the bacteriophage AB63 was added, the absorbances were 0.86 and 1.16 at 600 nm after 4 hours, and it was confirmed that the bacteriophage AB63 exhibited an excellent effect on the killing of Acinetobacter baumani bacteria.
실시예 6. 박테리오파지 AB63의 이미페넴 내성 아시네토박터 바우마니 균에 대한 용균활성의 확인 Example 6. Confirmation of lytic activity of bacteriophage AB63 against imipenem-resistant Acinetobacter baumani bacteria
분리된 박테리오파지 AB63이 여러 이미페넴 내성 아시네토박터 바우마니 균에 대해서 용균활성을 나타내는지 확인하기 위하여 아시아태평양 감염연구소에서 분양 받은 균주를 대상으로 감수성을 확인하였다. 소프트 아가 오버레이법을 통해 확인한 결과를 표 2에 나타내었다. In order to confirm whether the isolated bacteriophage AB63 exhibits lytic activity against several imipenem-resistant Acinetobacter baumani bacteria, the sensitivity was confirmed with respect to the strains delivered from the Asia Pacific Infection Research Center. Table 2 shows the results confirmed by the soft agar overlay method.
[표 2][Table 2]
표 2에 나타난 바와 같이 박테리오파지 AB63은 15종의 아시네토박터 바우마니 중 12종에 용균활성을 보여 이미페넴 내성 아시네토박터 바우마니 균(IMI-R Acinetobacter baumannii)에 대해서 용균활성이 탁월함을 확인하였다.As shown in Table 2, bacteriophage AB63 showed lytic activity in 12 of 15 acinetobacter baumanis, confirming that lytic activity was excellent for imipenem-resistant Acinetobacter baumannii (IMI-R Acinetobacter baumannii ).
실시예 7. 박테리오파지 AB63의 아시네토박터 바우마니 균에 대한 특이적 사멸능의 확인 Example 7. Confirmation of specific killing ability of bacteriophage AB63 against Acinetobacter baumani bacteria
다양한 세균에 대해서 용균활성을 나타내는지 확인하기 위하여 ㈜옵티팜에서 분리하여 보유하고 있는 균주와 미생물 자원센터 등에서 분양 받은 균주를 대상으로 소프트 아가 오버레이법을 이용하여 감수성을 확인한 결과를 표 3에 나타내었다.Table 3 shows the results of sensitization using a soft agar overlay method for strains that have been segregated and held by Optipal Co., Ltd., and those that have been distributed at a microbial resource center to confirm that they show lytic activity for various bacteria. .
[표 3][Table 3]
표 3에 나타난 바와 같이, 다른 미생물을 대상으로 감수성 검사를 진행한 결과, 박테리오파지 AB63이 대장균이나 Salmonella 종, Bacillus 종 등 다른 세균에 대해서는 용균활성이 없으며, 오직 아시네토박터 바우마니에 대해서만 용균 활성을 나타내는 것을 확인하였다. 이를 통해 박테리오파지 AB63은 아시네토박터 바우마니에 대하여 특이적이고 선택적인 항균제로 사용할 수 있음을 확인하였다. As shown in Table 3, as a result of conducting susceptibility tests on other microorganisms, bacteriophage AB63 has no lytic activity against other bacteria such as E. coli, Salmonella species, Bacillus species, and lytic activity only against Acinetobacter baumani. It confirmed that it showed. Through this, it was confirmed that bacteriophage AB63 can be used as a specific and selective antibacterial agent against acinetobacter baumani.
실시예 8. 박테리오파지 AB63의 pH 안정성 조사 Example 8. pH stability investigation of bacteriophage AB63
박테리오파지 AB63의 pH 안정성을 확인하기 위하여 다양한 pH 범위(pH2, 3, 4, 5, 6, 7, 8, 9, 10, 11)에서 시험을 진행하였다. Sodium acetate buffer, sodium phosphate buffer, Tris-HCl을 이용하여 각각의 pH에 맞게 조제한 후 990 μL로 분주하여 준비한 후, 5.0x108 PFU/mL로 준비한 박테리오파지 AB63 배양액 10 μL을 첨가하여 2시간동안 상온에서 방치하였다. 방치 후 각 반응액을 단계희석하여 소프트 아가 오버레이법에 의한 역가 측정을 시행한 결과를 도 6에 나타내었다.In order to confirm the pH stability of bacteriophage AB63, tests were conducted at various pH ranges (pH2, 3, 4, 5, 6, 7, 8, 9, 10, 11). Prepared according to each pH using sodium acetate buffer, sodium phosphate buffer, and Tris-HCl, prepared by dispensing with 990 μL, and then added 10 μL of bacteriophage AB63 culture medium prepared with 5.0x10 8 PFU/mL at room temperature for 2 hours. Left unattended. After standing, each reaction solution was diluted in steps, and the result of titer measurement by soft agar overlay was shown in FIG. 6.
도 6에 나타난 바와 같이, 박테리오파지 AB63은 pH3 내지 pH11의 범위에서 2시간동안 안정한 것을 확인하였으며, 산성 내지 염기성의 넓은 pH범위, 특히 pH4 내지 11의 범위에서 2시간 동안 안정한 것을 확인하였다.As shown in Figure 6, it was confirmed that the bacteriophage AB63 was stable for 2 hours in the range of pH3 to pH11, and was stable for 2 hours in the wide pH range of acid to basic, especially in the range of pH4 to 11.
실시예 9. 박테리오파지 AB63의 온도 안정성 조사 Example 9. Temperature stability investigation of bacteriophage AB63
박테리오파지 AB63의 온도 안정성을 확인하기 위하여 다양한 온도 범위(40℃, 50℃, 60℃ 그리고 70℃)에서 시험하였다. 박테리오파지 AB63 배양액(5.0x107 PFU/mL) 1 mL를 멸균된 튜브에 분주한 후 10분, 30분 및 60분 동안 각 온도에서 방치하였다. 방치 후 각 반응액을 단계희석하여 소프트 아가 오버레이법으로 역가 측정을 시행한 결과를 도 7에 나타내었다.To check the temperature stability of bacteriophage AB63, it was tested in various temperature ranges (40℃, 50℃, 60℃ and 70℃). 1 mL of bacteriophage AB63 culture (5.0x10 7 PFU/mL) was dispensed into sterile tubes and then left at each temperature for 10 minutes, 30 minutes and 60 minutes. After standing, each reaction solution was diluted in steps, and the result of titer measurement by soft agar overlay was shown in FIG. 7.
도 7에 나타난 바와 같이, 박테리오파지 AB63은 40℃ 내지 60℃의 범위에서 60분 동안 모두 안정한 것을 확인하였다.As shown in FIG. 7, it was confirmed that the bacteriophage AB63 was stable for 60 minutes in the range of 40°C to 60°C.
비록 본 발명이 상기에 언급된 바람직한 실시예로서 설명되었으나, 발명의 요지와 범위로부터 벗어남이 없이 다양한 수정이나 변형을 하는 것이 가능하다. 또한 첨부된 청구 범위는 본 발명의 요지에 속하는 이러한 수정이나 변형을 포함한다.Although the present invention has been described as a preferred embodiment mentioned above, it is possible to make various modifications or variations without departing from the spirit and scope of the invention. Also, the appended claims include such modifications or variations that fall within the spirit of the present invention.
이하 본 발명의 박테리오파지 AB63을 포함하는 감염성 질병의 예방 또는 치료용 약학적 조성물의 제제예를 설명하나, 본 발명을 한정하고자 함이 아니고 단지 이를 구체적으로 설명하고자 함이다.Hereinafter, a preparation example of a pharmaceutical composition for preventing or treating an infectious disease comprising the bacteriophage AB63 of the present invention will be described, but the present invention is not intended to be limited, but only to be specifically described.
제제예 1. 사료 첨가용 조성물Formulation Example 1. Composition for adding feed
박테리오파지 AB63 1x109 PFU/mLBacteriophage AB63 1x10 9 PFU/mL
비타민 믹스 100 mg
미네랄 믹스 100 mg
스테아린산 마그네슘 2 mg
통상의 사료 첨가제 제조방법에 따라 상기의 성분들을 혼합하여 제조한다.It is prepared by mixing the above ingredients according to a conventional feed additive manufacturing method.
제제예 2. 소독제 조성물Formulation Example 2. Disinfectant composition
박테리오파지 AB63 1x109 PFU/mLBacteriophage AB63 1x10 9 PFU/mL
에탄올 적량Ethanol
멸균 증류수 적량Sterile distilled water
pH 조절제 적량pH Adjuster
통상의 소독제 제조방법에 따라 상기의 성분들을 혼합하여 제조한다.It is prepared by mixing the above components according to a conventional disinfectant manufacturing method.
제제예 3. 세척제 조성물Formulation Example 3. Cleaning agent composition
박테리오파지 AB63 1x109 PFU/mLBacteriophage AB63 1x10 9 PFU/mL
에탄올 적량Ethanol
효소 복합물 적량Enzyme Complex
멸균 증류수 적량Sterile distilled water
통상의 세척제 제조방법에 따라 상기의 성분들을 혼합하여 제조한다.It is prepared by mixing the above components according to a conventional cleaning method.
이상, 본 발명내용의 특정한 부분을 상세히 기술하였는 바, 당업계의 통상의 지식을 가진 자에게 있어서, 이러한 구체적인 기술은 단지 바람직한 실시양태일 뿐이며, 이에 의해 본 발명의 범위가 제한되는 것이 아닌 점은 명백할 것이다. 따라서 본 발명의 실질적인 범위는 첨부된 청구항들과 그것들의 등가물에 의해 정의된다고 할 것이다.As described above, a specific part of the present invention has been described in detail. For those skilled in the art, it is obvious that this specific technique is only a preferred embodiment, and the scope of the present invention is not limited thereby. something to do. Therefore, the substantial scope of the present invention will be defined by the appended claims and their equivalents.
SEQUENCE LISTING <110> Optipharm.CO.,LTD <120> Novel Acinetobacter baumannii specific bacteriophage AB63 and antibacterial composition comprising the same <130> optipharm1-80 <160> 1 <170> PatentIn version 3.2 <210> 1 <211> 87957 <212> DNA <213> Artificial <220> <223> bacteriophage AB63 <400> 1 tgatctttag cttgagtaat caggtcagca agaaagtagg gtgtgcttta agatcaggta 60 cccatacttc accggtttca cccctaaagc acctgagttt tcgcatgatg tgtaggcgaa 120 ggtttgaaat taataacgcg gcatttttac cttcaccagt agtaacagtt gttagataac 180 ccatgacatc tgcgatacgg taaagctcgt tacggttttt accacctaga tctggcggta 240 aataatttga tcaccgtttt gatcttctga tgcgtgtgca atgaaaacaa catctttacc 300 taaactgatc aaagtatgat gtattgcttg aacgtttggt tcgctaaacc ttgagccttt 360 aactttaaag agccatcttt ttgacggtta ttagcagtta gcaatagatg ggttttaatg 420 cattcaagca ttgcacccac ggtatcaatg actacggttt tatatggtgc taagtcctgc 480 ggagtaaggt ttgcaacatc actccattgt tgaacctgta caaccgcacc acgacgtaat 540 tcaccagtac ggtgagcacc acggtcaaag tcaaaagaaa ttgctttttc cgcagtaaag 600 cccatcgatg atttacctaa acccggatca gcgtataggt acacaataat tgcttgaacc 660 aataaagttt ggtcagcagt aataatcggt aacgccattt ttcttatcct tatcttgagc 720 cagtgaagcc gcgcttagtt ttataagcct tgcggtcata agtagggatg tttgtttcac 780 gcagttttat tgcgagctgc tttctgcgtt ggaaatcaat ttcttgtgtg agttcattcc 840 aaacttttgg atagtcagtt tggaacctga acacatttaa aggcgtctta aatccgtctt 900 taactttgta aagaactgag ccattagcat tagatgcgta cacttgccag ccgatgcgga 960 cagagtagag gcccttatca tcacggccta aaaatgactt gtagccgtca gggtgttttt 1020 tgaaattagt catctttaag cctgcaccaa cttgttacgt tcgatgaagc cttttagaag 1080 gccattgatg tttcggatgt cttcaaattc ggtgaaatcg ttatatgact taccattaac 1140 atcagtgatt tcatttactg tgagttgtgt aatatcaaca gcagtgaatt cagaacccgg 1200 aacgccgtag ctgtctggat gagcttcaaa atcaaagcta acgtttaaac ggaagctatc 1260 taatttaatt acagcaacgc cagaatgttt acctgtgatt ttcgcggtta acacaccgta 1320 agtacttggt tgagttttag gtgtaaaaag agtaggtgct tcttttgttt ggaaagctgg 1380 ctgcaattgg caagcaacta aagaacctcc agagattgca agagcagcca tgctgacaaa 1440 tgcaaatgag ttgaaagggg tagcttttac gttcataatt gatctcgcag tttgcaaaag 1500 cacatcagat ttagcggtcg gtgtgctttt ttgttgtcta cgagacaaat atagttttat 1560 ctattattag tgtcaatagg tataactata aaatgatagg tgtaaatttc taaattgata 1620 ggtaaatcta taagtaaaaa gcccacctta taggtgggca aatgtataaa attgtttcta 1680 taggtgaatt tattaaccag ctcgccaaaa ttgacgaccc attaccttaa aattttttcc 1740 attctcttca gtaacttttc gatctctaaa cttttcattg agactatgaa gaattagaga 1800 cccatcagcc tctttaaaaa tttgcttcaa cattccttca ccagcgaagt aaacagcata 1860 tatttcaccg tcaataactt cagtttgaga aatatcaata ccaactaagt caccatcttt 1920 gatgtaatcc atcatgctat cacctttagc tttgatgata cgcatgcaat cctcagagac 1980 acgtttttct ttaaaaaatg atggtggaaa agggattgct ccattgattg catcaaaatg 2040 aaattcaata gattcacctg gtccacatga aaaattagct tccactacgt caatccaaat 2100 gtaattgtct attcctttat gaaccacttt aggttttaca aaatcatctt cggaaaaacc 2160 atcttcagtt tttccagtag ttagccaata cgcatctaca cctaagtatt ttgcaatcag 2220 tggaaggaaa gcagacttca agctcttacc actttctaag gcttgatatg tgggttgttt 2280 aataccaact gcttcagcta cttcatactg acttttacca gctttagtac gagcttcttt 2340 gagtcgagtt gccaaatcag acataacaaa aaccttaatt tgtttggaat tggattttat 2400 aggctcaact ataaaacttc aaaaaggcat acctattgac atatgatagg tataactata 2460 aattaactat caaaatagga gtttgatagt tatgagccaa aatctcatcg caatctatga 2520 aaaattagtt gagttcttag gtggccaaac aaagacagct caagtcctac ttataaaaca 2580 accctctgta aatgcatggc ttaaaggaaa aaaccaaatg tccgaaaaga ttgcattacg 2640 cgctgagcgt gtaactaaag gtcgttttaa agcttatgag ctttgtccat cactttcaga 2700 attcgaaaca ttaaccgctt aggaccttac ccatgagcaa agtatcaaat gaattgcctg 2760 caagcgctag caataacgaa tcgctcatat tgcaagcact taacgctagc aaccaaagac 2820 aagtagcaga gaaggtggga atagatgcaa gcaccttgtc gagaatgaaa aatgacaaga 2880 aaaacaatgg attgacagag attgaattta ttagcttttt gttgacagcc attggtttga 2940 aggttgtgca agagagtgat gtgtattgct cacctgaaat tgcagaagca acgcgagttt 3000 atttagcaca tgcattcact tcacctgaat acatgcggat tttattcaaa taaaaaacca 3060 ctacctgcgg gaacaggagt ggttaggcat tcaaatgagg tggatcaaat gaacacaaac 3120 aatttatcag aacaaccaat cgaactcaac tcaccagatt tttaataggt gacgttgtag 3180 tgcttactaa agagtgccgt actttcaaat caaatgattt gtttgaagtt aaaaataaaa 3240 ctttgactag tttatggact atcaaatcgg agaatcattt gattctggtt tcatcaaaag 3300 aaatccgtac agcaacagta gcagagctca acgctaaacg ccgcctaaca aaagctgagc 3360 aagcattagc ggaggtgtca tgaatagtca atttaagtat aaacctgagt acaaacagac 3420 tcaggaaatt cagtccttct ttgatccagc gttagtgatt ctcaatgagc tacatgatcg 3480 taaccgtaaa aatctaagag ccaaaggtta tgacgaaaat aatgctgcaa taacgcgtga 3540 agaattttca caaactatgg cacagcgttt tcgcattaat cagtggttag cagggcagat 3600 cgttaatagt ttggctaatg ctgacttggt tcaaaaattt ggtgggtatg taaagcctaa 3660 ggtcggtgta catgagtaat tttgtgccta attcctttca agtgcctaat gcatttgttg 3720 acgaggtttt aaataaaatc tctgatgctg catgcaaaat ttatttagtt atttgccgta 3780 aaactcgtgg ctggaataag gagatggatt ccatctcttt aactcaattt gaagagatta 3840 cagggaagag tagaccgaca gttgttaaat gccttaatga attaattaaa gttggtttag 3900 tcgtggaaca accaagcaca attcatggaa atacattcaa attaggtaac gatactagcg 3960 ttggtttaat gcttaaattc cctagtaaaa attttttact acctgaaatt tatggccaaa 4020 ctagtaaaaa ttctttacca ctgctagtta aaaattttaa ctacactagt aaaaattttt 4080 taccgctact agtaaaaatt tttaacacac aaagtatcac tatcaaaaac aactctcaaa 4140 gtaataaaaa ataaataaaa aaagagagcc tgtttctgaa aaacctaaat cagaaaaacc 4200 aactgaattt aatccacgtt tagttgaact accagcatgt gtagatccag agctgtggaa 4260 caattttgtt gatatgcgta tcagcatcaa aaaaccactt tctgaaaatg cagtaaagct 4320 aatccttaaa aaacttatct cgtttggccc tttggctaac caatcactgg aaaactcaat 4380 tatcggaaat tatcagggtg tatttgagcc tcgccaaaat caaattcagg aaaacccaca 4440 atctcataac gttcctgaag aaccgggtta tttcactcaa atgtacgctg agagcaaccg 4500 ttcaaacgtg attgacgtta cgccagtgtc acaagatttt ggaggctatt aatcatgaat 4560 gaattagcac catttgaaag ttttttaaaa gaactaattg cggcttacag aactaaatac 4620 gctgttcagt tcaataagaa ttttccagta gaggggaaaa atgccgttcc aatgcaaatc 4680 gttgaacagc agcttgctaa agcattggtt ggggttacac ctaaccaact tcaaagaggc 4740 ttagcgctat tttacgcaag tacaaataca tacatgccta acttcgctga attccgtgct 4800 atgtgcatgg gtgatgattg gtggagcgct gagaaggctt gggttaaggc ttgtgaatac 4860 actcagatct ctcaacacaa aaaagtgaca ttgccagacg gaagagagca gaaccaagaa 4920 attacaacct tgaccaaatt tgttttagac caagtttatt cactaatcca agatggtgaa 4980 atgtacaaag ccaaaatgga atttatcaaa atctatgatg aatacaaggc tgaagcacaa 5040 ctgaagggta aaacccaagc ttggtaccaa gaaccaattt tattagctca gaaaaatgag 5100 caaaaggtgc ataagcctgt ttcaaatgac gaagcgcaaa agcatctcca atcattgatg 5160 gaacgtttaa aaatcaatgg tcgtaaacct gtaccagtac aaaagcttaa ggctaaggaa 5220 aaagagccag aactcaaaca agagctaggt ccagatcctt ttgacaatcc gcacgaatac 5280 gcagagatgt gccgccgtga aggtatgcca attcctagaa atattcttaa gttaattgat 5340 ggggcgaata tatgaataaa ttcgagattt tagcgtgggg tttactcatt tcatttttta 5400 cagcagctat tagcggtgcg gtggtttggt ggtggttggc gcgtaaagag cttgatgaga 5460 aaggagccag ccatgaaagc aactaaattg attagagata aaggactgca atacgcgaag 5520 gaaatcgtag attcagcacc cgataacgca actgaatgga acgagggtta tgagttccaa 5580 tgtggtcaaa gtgtagaaat cagcccagca gatcgtgaga agtattttgt agatttggtt 5640 gagcttaaac gtctggtgga gtctttgaaa atcatcaacg atttaggtgg agttgagaag 5700 ctaacgcctg cattcattac gacagataag catgttggtt acacgcatgt tcgcatggtg 5760 ggaaatggga gattgagctt tcttgatgat ttttgcgact tcattccaga tggttccatt 5820 tcaattaagc gtgtgatgac tgctatccgc gaccacgaat caatatacgg aggcggtgaa 5880 tctcatgcca actagatata acacaggcga gtatagctac gatcttgaat atcactatgg 5940 agatatgtca gcaagcatgg agatgcttag agcacgttta attgaattgt tgactcctca 6000 tctgtctggc cgttatgtga aatggagaga agcatatttc acatggttta caaagtgcgg 6060 cggggattcg gggtggatgt tttgtgtagg tccacacgaa tttcatattg atggggcgtt 6120 aaggcgctat tactcaggtt ctattgatat tacctacaac cagaaagatc gatgtttctt 6180 ggtgggtgag aaaaagaaag tcaaatgtaa ggcttgtaag gggtttggct tcattcgaga 6240 tgatgggtgg gggcatatag ataaatgtga aatgtgtgat gcagaaaaag gagccagcca 6300 tgagtgagtt tgagggtaaa tctggaaagt gggcttggga gattcaaaaa gaacaacaag 6360 cgaaagtgga ggagctgcaa aagcgtttag atggggcatt aaaagagact caatatgctt 6420 tgcagtatgt tgaagaagac atgcgcggca atcatgaatt tctacaaatg gcaatgattc 6480 gaacccttaa agctatagag caagtgctca aaggtggtgc ttgatgtcat cagtcagcat 6540 tgctgaatac cgcaagttat ttcccataaa gaaaaataaa aagcgccgtt cagcaaagca 6600 agttgccaga caaccaagtg tgggtgaagt ggttctggca acgcatttaa gagcatgcaa 6660 gattggattt gaacaggaat ataagttcca tcctgaacgc aaatggagag cagatttttt 6720 aataaagggt tcaaagattt tgattgaggt agaaggcggg atctggagcg gaggccgtca 6780 cacaagaggc aagggctatt taggggatat ggagaaatac aactccgcag caatgatggg 6840 ttttacagtt ttacggttca gtacagagca agttaagtcc ggtatggcat taaagcaaat 6900 tgaattatta attaagggta aataggaagg cgattatgtt ggttgaaaag tttgatttta 6960 ttgagttact tcgccttgct attgctcaaa gcgaaggtaa agggaaaatt actaagcatg 7020 ttgttttggg agaaattgcc ttattgcctg caggtgcaaa aaaatgggca gaattactgc 7080 ttgaacgtat tgattttgag cgcattgcag aaatcacaga aacaaagaaa atttatgaga 7140 ccaggataat taatggtaag gaatcaaaaa agcgtattgg tgaaataccg ggtaaagttg 7200 aaataaaaaa aggggagatt aactcagctg attttttccg cgttagaaac gtactagcgg 7260 gtaagatcca tcgtgaaatg atcaaaaaga actttaagcc aaataattgt cagggcgatt 7320 tatcaaatgt ggccaaaggt attgctgagg ttgttttgcg tgggcgatta tttacaaagg 7380 caatgtgtgg ccattgccag ggattaggca aattggagtt atttaatgaa aagggatatc 7440 caagcggatc taagttttgt gataaatgca gtggtacggg gaaacgccca tatacattgc 7500 atgaaaaaat tacgatcgca aaattaaaag tgtctaagtc tggttattct gagcgatatg 7560 aaccatatga attaattgct gaagcatgta tcgaaaattg ggaaaacacc attagaacta 7620 gcctggctag atcgtttcat tttgaacctg aagaaatatc attagcttga cttaaacaga 7680 acggttgagt ataagtattt ctaaaatggg cgctttatac atggatcgcc tgaaaaactt 7740 aataaaagct cactaatttt agtgggcttt ttgcgtatct agagcattga aaatggaaaa 7800 cacctggcat gctgaccaag aaaaaccaga attacggcca gatgaaaaac ctttgaattg 7860 cccattttgt ggatctgatt caatttgtac ggattcttca cattatggaa aaccagatga 7920 agacggctct atagcgtggg atgctttcac atggtgtcat gattgtggat caaaaggccc 7980 tagtgcttgg gcgatgatcg cttgggatga aaattttcat tacgacactg tttatgaaga 8040 aagatcaatt gttaattatg ctattcgcca gtggaataca cgcaaataag atttttaatc 8100 tcgtgagggg tgttttataa gcacacctct cttttagccg gacggattac ggcgcaaacg 8160 gcccgctaca tactagttat tggcggggct ttttcttttt ggagtatgta tgactgaatt 8220 tcaaaaaatt acgcatgaga ttagacagct ccaagtagag ttaaatcatt taggaagctg 8280 caatacaaaa gacttaacag cagaacagat cgctcacata gatgagcgat tttttttggc 8340 cattgaaaag caaagtaaat taattgctcg tctcaacaat aagccagagg gcttcttata 8400 aatattggtg gtgcgatgga tcctaaaaag tactatatgc tcaccaggaa aaaagaaaga 8460 aaacccaagc caaagagcac accattacca aaagccaagc aaaactattt agaggccgaa 8520 gcaactctaa aagaagaatt aatcgagttg gccattggtt ttgaaagtaa gtttcagccg 8580 atccatacca aacactggcg ctttgatttt catattgtga aattgcgttt gctcattgaa 8640 attgagggtg ggccctggtc tggtggacgt ggtggaaagc tggcaaataa agcatggagt 8700 cttgatcgat atgatcaagc tgaagagatg ggttataaaa tagagcgctt tcatccagat 8760 tctattttgt cgggatatgt catcaactgg ataaaaagtg aattagcgag aattgaagat 8820 ggagcaaata agaccatttc caccgactga ttttattgat caagcagatg aagaagaagc 8880 aattagacta acaccagcac cagatctaaa aaaatgggtt gttgctaatt acttaactat 8940 tggtggacct ctttataacc ccgatcatga tcacataggt gaactgcttc acgataatga 9000 agaattttta gcattcgcgt gggcctcttc tgcatataaa agcaagcaag ctatggtgtt 9060 gggccagtgc gaaaaagtca tgttcaatgt cggtggctgg cgtaaagctc gacaagagca 9120 acagatgcga gactggttcg gctttgtgcc aacttactta ataactgtcg acgcttcttt 9180 ttgtgagcgt gcaaatgata cagagttctg ttacttgctt gaacatgagc tttatcacat 9240 tggagtgatg agagacgagg acggagaaat tgtttatagc gatagttctg gtcttcctaa 9300 gcactatctt gcaggtcatg acgttgaaga gtttattggc gtagttaaac gttatggccc 9360 aagcaaaaat gttaagcgac ttattgaagt cgcaaagaat ccgccgtttg tttcgaatct 9420 tgatatttca aaatgctgcg gaaactgtgt aatcaattga gcctaatggc tctttttttt 9480 gcccattttg ttatacgtag ttatacgatg aggaagttat ggcgacacta aaagagcctg 9540 tgaaaatctt tatagttcag tctcttgctt gtcgtgatac acctcaagaa gtggctgaac 9600 tcgtaaaaca agagtttggc gttgatatag atcgtgttca agttgcaact tatgacccta 9660 caaaggttgc tggtaagaac ttaagcaaaa agtatgtcga actatttgaa aaaaccagag 9720 atgagtttga taaaggctta attgatattc caattgctaa taagtactac cgattgaagc 9780 aataccaaag acaacttgag aagactagaa acgtcaaaac agccttaaaa attcttgagc 9840 aagccgctaa agacattggt ggtcaattta ctaatcgcca agaaattaca ggcaaagacg 9900 gcggaccagt ccaaacagtt aattcagaaa ttccagttcc aatggaagat tacttaaaag 9960 cgcggaggga agtcttagat gagtactgat gcggctcggg ataaagccat ccggatcgag 10020 gcgcaagaag atttatattt cttcacaagg tacatgttta aggagcgccg tggttataaa 10080 tggatgcaaa attggcacca cttagaaatc tgcgaagctt taatgaaagt ttatcgcgga 10140 gagataaagc ggttaattat taacgttcca ccacgatatt ctaaaactga aattgctgta 10200 attaatttca tggcttggtg ttttggtaag aatccagact gtgagtttat tcatatcagt 10260 tactcggcaa tgcttgccgc aaataatgcc ttccaaatac gaactcttgt acaagaagag 10320 gcgtatagaa aagtctttcc tgagcttaca ttgcgtgatg atagtaaggc taaagacttc 10380 tggagaactt ctcaaggcgg tgtctgctat gcgacaggta caggcggtac gattactggt 10440 tttggtgcag gaaaacttcg taaaggcttt ggtggctgca ttattattga tgacccacat 10500 aaagcacatg aagcttcatc aaaaactatt cgagaagggg taattgattg gtttcagaac 10560 acactcgaat cgcgtactaa ctcgccagat acgccgatca ttgtgattat gcagcgactt 10620 catgaagatg atttagctgg atggttgcta ggtgatagaa aagacggcgt tcctgtagct 10680 ggtggtaacg gtgaagtgtg ggagcatcta tgtctttcag ctattcagga agacggatcg 10740 gcactatggc cagcaaaaca caatattcaa aaattgagac taatggagca agcggcaccg 10800 tatgtatttg ccgggcagta ccgacaaatg ccatcaccgc cagcaggcgg tttttttaag 10860 cctgacaata ttcaaattgt tgaggctttg cctgcagatg tattgaaaca agttagggct 10920 tgggactttg gggcaaccga aaatgaaggc gactttacag taggtgtgcg agaagctcta 10980 ggcgcagatg gttttactta cattgtcgat gttacaagag gacagcttgg tccagacaat 11040 gtgaataagc gtttagaaca aacagcaaag ctagatggga aaaaagtttc tgtgcgttta 11100 ccacaagacc ccggtcaagc aggcaaatcg caagctagtt catttgtgaa gcttcttgcg 11160 ggttatagcg tgatagctaa gccaatttca ggtgacaagc ttacacgtgc acaaccattt 11220 gcggcccaag ttaacgtagg aaatgtacga atgctcaaag gtgaatggaa taaggatttt 11280 attgatgagc ttcgtcattt tcctaatggc acacatgacg accaagtgga tgcagcttca 11340 gatgcgttta atgaattaca tgaaggtttt gaagccttct ttgctgatat gggatttgct 11400 cgatgagtga tgtaactttt caacatgctg aatatgttaa gaacttgcca tactggcaaa 11460 aacttgatga tgtttgtgaa ggtgaagatg cagttaaggc taaaggtgaa aaatatttgc 11520 cgatgccaaa tgcacatgat aaatcacctg caaataaaag cgcttatgag gcttatctta 11580 cccgtgcagt cttttatgaa gtaacaggga ctacattaaa tagtttagtt ggtgcagctt 11640 ttgcaaccga tccaagtttt aaatttcctc cggaacttgc tcatttagaa cgtaatgcaa 11700 atggtgctgg tttaagtact tatcaattgg ctcaaaatgg aattcgccat ttattgaagc 11760 attatcgttg tgctttatat gtagattatc ctgatgtgcc gccagctcgt aatctagcgg 11820 aatttaaagc acaaaaagcc tatccgatga ttcatttact aaatgccctt gatgtagtga 11880 attgggattc agtaatgatc gataaccaga aaaagctttg cttagtggtt atacgtgaat 11940 ttaagtctga gcgcggtgct gatggattta gtaaaaccga acaagagcaa tatcgtgtac 12000 ttcgtttaga gcaagaggga aatggggaat atatttattc cgttcaggtg tacacaaagg 12060 gtgaaaaggg taactgggtt ggcggagaga agaagtttcc aacagattac aacgggaatt 12120 tctggaccta tatacctttt acatttgtag gtgcaattga taattcagaa gagattaaaa 12180 agccaccatt acttcctttg gctaatctca atttagccca ttacagagac agtgcggact 12240 ttcaagagtc cgttttttat atggggcaac ctcaatatta tgcgaagggt gttaattggg 12300 agtggtatga ccaagccaag aaacgtggca tctacattgg agcgaaagta cttttgcctt 12360 tacctgaaaa tggtggttta ggaattgtac aagccgaccc taatactctt gcccgggaag 12420 cgatgaaaga taagtgggaa aaaatgaagg agatgggggc gcgtttaatt gagaagggct 12480 cgggaagtaa aaagaccgct accgaagcga atagtgatga cgccgttcag cattcagttc 12540 tttcgctctg tgtcgttaat atgaatgaag ccttgtcagc agcattacga tgggctgcta 12600 agtttgtaac gcctaatgtg gatgttctaa ctaaagatga tttgatgttc gaaatcagtc 12660 aagaatttaa caaacagggt tatttagctg agttagctcg acagttattt gaagcagctc 12720 tacaaggccg atcttcattt aaatcatggt gggaatacaa ccaaacaggt atgttcccta 12780 aacaaaaata tgaagaagag cttcagaatg ttgaagcaga gcaagatggg actttaaatc 12840 aaaaggtaga gtgagatggc aacagatatc aaaaaactat ttgaagtact cactcagcac 12900 caggcctatc tttatcgtgc ttcatcaaaa acggtaaatg agttattggc tttattcaat 12960 gatgatacga gcaagatgct atctaagctt cgggatttat tggatgagct taatgagtcg 13020 gagaaagttg ctttagctgg tggtaaatat acaacttcaa atttaaggga aattagggat 13080 ttgattgccc aatggtttgc cagtgttaat ttagcattac ctgaagcttt tgccgtttct 13140 gctacggcgc tggctgttta tgaggccaat tacgtagcta agctctatgg agcaaaaatt 13200 aataagcctg atggggaaaa actattctta tccgctaaaa aagttccgtt ggcaggtggc 13260 gctcttgtcg atgatctgct ttcaagaatt gctgaaagtg cccgtcaaaa ggttgagtat 13320 gcaattcgag atggtattaa ttcaggcaaa actaaccaag aaattgttca gcgtattcgt 13380 ggtaccaaac ggcttaacta tgaagatggg atcttaaatg gtaccaaaac tgatattgag 13440 cgaacggtaa gaactgtgcg aagtcatgta gctaatcaag cctatctaaa tagcttcaac 13500 caaattggct ttgaatatgt ccgatttgtt agcgttttag atggacgaac ttctaagctt 13560 tgcgcttcat tagatggttc agtgtgggaa ataaatgatc cggcaaagcg agtgccgccg 13620 ttacatccta actgtcgcag tatcttggtt ccggtcgaga aggacggtca acttgttggc 13680 gaacggccat ttgtaatgga cgaacgtaga gttaaagaca tccccaaaga agagcgaagc 13740 cagttaatag gacagttaga tgcaaacacc acattcaaag agttctttaa gaaaacagat 13800 gatttctttc aaagggagtg gctagggcca aagcgcttta agctctataa agatgggaaa 13860 tttgattttg ataagttctt tgatcctgaa ggccgtttct atagcttaga tgatttgaga 13920 aagttggatg aaaaagcttt taaaaagttg ggtctgtaat ttttcttatg ttatattttt 13980 taaaacatca gaatttatac aatatgaaaa caatagcttt tgtatgtcta accctaattt 14040 ccatcacttg tttagctgaa ccaagtcaaa aatatcttaa agaatatgat cgattgtctg 14100 aagctttgga gtcagcaatg gcaaatgcat attcttttga tcctgcaact ggtcaagtaa 14160 aacaggctac tcaaggttta gaagctaaaa ataatttatg tagagctgcc caggcgaaac 14220 taaacctcac cacgttttta aaagacaatt tagaggaatc taaagagctt tataaatcta 14280 ttgatggtgc agagactcta gataaaaatt atcttagtgg acaacagcag gaacaacaaa 14340 atctcgtttc aaatttgaaa aaagaccttg ttggaactgg atttaactgt gagtaattat 14400 tgccgattac aggtaattct aaactcactt aagacacaat tttcacctat ataagcgccc 14460 aaatggcgct tttgtcattt atggagtttg gcttatgagt gaatcaaaag ttagacattt 14520 ggtacttaaa agagtttcag ataaatcttc tcatcttgct ctttgtgacg aggaaacagg 14580 tattccatta gctggattaa ccgctgtaaa aatgaattgt agtgtttttg agggtccagc 14640 gactatcacg gcaacatttg atgtaggtgg tcctcaaggc atccgcttag ttggtgatga 14700 acctagacaa aaggtttgga gtgcaaagga aacgtagcga aaggcactac aaatgcctga 14760 aaagcaaatc aatatgtcag atgctcaata tattctgagc acaaaatgaa ttctggtgcc 14820 atttcttcaa attaaggttt caagccatgg caatttatgg ttttactttt gaaagattaa 14880 aagcaattgc actcatcaaa tagaacttaa tttttaacca tagcaccttc gggtgctttt 14940 tttgcgagaa gaaaatgcca agccctatta tccaatattt ccaatatgaa catttacctg 15000 aacatttgca gcaagttagt aagccaattg gtgatttagc tcggcaaatg gatgagcaac 15060 ttcctgacgg gcctgaaaaa tccacaggat taagaaagct acttgaagca aaagatgcat 15120 ttgtacgcca agctttaagt aaataatcat ttatagaaat gaagcgtcct aaagggcgct 15180 tttttattgc ctgccgaaag cggatgctaa cggcgaatcc gggcggatgc ccattttgta 15240 tatataggtt ggatgaccaa tgaaacttaa aacagtaaca atcgacggta aagtttatgc 15300 ggaagtagac ggtgataagc cgatctatat tcatgatgac ggcaaagaaa tgccacatga 15360 tgcaccacac tcggtagcaa caattgcacg cttaaacaat gaagctaaaa cacatcgtga 15420 agccaaagaa gcagccgaaa aagcattaaa agcttttgaa ggaattgaag acccagcggc 15480 agctaaaaag gcattacaaa caatccaaaa tctcgatgat aaaaagctgg tggatgccgg 15540 tgaagttgag aaagttaaag ctgaagctat caaagcagtt gaggaaaaat atgccccgat 15600 tgttgcgcaa cgtgatgctc tagaagcctc tttacataaa gaacttatcg gcggtggttt 15660 tgctcgttct aagtacattc aagacaacat tgcagtacct gtggacatgg ttcaggcaac 15720 ctttggtcat cacttcaaaa tcgaagaagg caaggtggtt gcatatgatc cgaacggcga 15780 aaagatttat tcacgtgtcc gcccgggtga acttgcaaat gttgatgaag ctttagagtc 15840 attggttggt ggataccagc ataaagactt aattcttaaa ggtggtaaag gaactggtgg 15900 cggttttcaa ggtgggggca aaggtggagc acctactgga atgaaacgca gtgaaatgtc 15960 tgtttctcag aaagcagatt acatcaaaga acatggcaat gatgccttcc taaaactacc 16020 gaactaatca ttaaatattt ggagataagt agttatgact acgacagtta attcagacat 16080 gatcatctat aatcaattgg ctcaaactgc ttatttagag cgtttgcaag ataatttgaa 16140 tgtatttaac caagcctcta atggtgcaat tgtttatcgc aatgagatca ttgaaggtga 16200 tttcaataaa gaagcattct acaaagtggg cggtagcatc aaacatcgtg atgtgaattc 16260 aaccgccaaa gtagttccag agaaaattgg ttctggtgag tctgtaggcg taaaagtccc 16320 atataaatat ggtccttatg catcaactga agaggcattt aagcgccgtg ctcgtacacc 16380 agaagaattt gctatggttg ttggttacga tcttgcagat gcattggttg caggccgatt 16440 agagtacagt ttagcttctt taaaagctgc tatttctagc aatccagaca tggttgcaaa 16500 aggtagtatc gttgttgatg gccgcaaagc attgactcgt ggtatgcgaa agtttggtga 16560 taagtttggc cgcattggct tatgggtgat gaactcagat acatatttcg atattgtcga 16620 tgatgcaatc actaagcaaa tttatggtga atctgaaatc gttatctacg gtggtttacc 16680 gggaacctta ggaaagccgg tcttggtgac ggacgctgta ggtgataacg atgcttttgg 16740 tttgcagtat ggcgctgtca ctgtaactga atcacaagta ccgggcttcc gagcttatga 16800 catcaatgat gaagaaaact tagcaatcgg tatgcgtgct gaaggtgcat ttaacttaga 16860 tattcttggt tatagttggg atacatcgaa aggtgaaaat cctgacctta cattacttgg 16920 ttcaagcgct aactggatca aatatgcaac cagcaacaaa atgacagcag gtaccttact 16980 tgatttatcg ggtacagcga caactggtta aaacctaaaa attaaaacct aaggggctaa 17040 taagccctct tttttattat taagagaaaa gcgccatgaa gattatctat acacgcattg 17100 cagcagcggc tgcattagag acaggcatta ttgctaaccc tgactattat gaaaacccaa 17160 atttgaaagc aaaagaggta attatttacg gtaattatcc aaagattcaa aaggattatg 17220 aatctttgga agttccagtt gaagttcgta agttggaagt gccacaaaaa acgactttgg 17280 ccacagtaaa tgtcgcagtg ggaattaccc ctgaacttca agctgtgatg gatgatgcaa 17340 aagctgaatg tgaaaaggta gttgaagaaa acactcagct taagcagaaa attgccatct 17400 tagagcaggc cggtggtaac cagtcagagt tgttatctga aaattcacga ttaaaagatg 17460 cagcagtctt agcagataaa gctctcaaag atgctgaagc tcaagtggtc ggtataaaaa 17520 ctgaatttga agcttttaaa aatgatattc ctgcaatgca ggcacgtatt gctgaattgg 17580 aagctggaaa agcggcagaa aacccagcta cagaaacggc agctaatgat tttgaaaact 17640 ggtcaaatga tcaattaaaa gagtatttgg ctagtaaaaa cattggttac aagccgtctg 17700 caacaaaagc agaacttctt aaattaatcc cgaaggaata atgcaatgag ctttattact 17760 gtagatgacg caaattcaat tttgggcagc gattttgcac cagacagtga taaggctcgt 17820 ctggtgaagc tggcaaatgt gtggatgaaa aacagaattg gttttgtacc agatccaatt 17880 gatccacttc ttaaggacgc ggcttgtgaa attatcaaag gaattctggc caaagtaatt 17940 tataacggca aagagcagca gttgaagcgt aagaaagtta aggctgattc tgttgagtca 18000 gaaaaagaat ttcaagacgg atctgaagca atctctagct ttgaacagat agcaattgat 18060 tttattgatt cacttgattt gaaagatcca aatgcaagtt ttaatggctt tggcatacca 18120 ctttacaggg catgatatgg gcttacgtga cgaaattcag gcagacattg ctgaagcatt 18180 taatgatgat ttagcagatg ccattcatac ctttacatgt gagcggatct ctaaaacgaa 18240 ttgggatcct aaaactgaaa cttatgttga agttaaagaa aactattctg gccgtggcgt 18300 tctgtttggc tcatacagtc aatatgagat ccaaacactt ggagtactgg ccacggataa 18360 aaaggcaacc gtgcttcaaa atgaagtaac tatgactcca aaaattgaag atgaatggtt 18420 aactgcctta ggctcattcc gggtaattaa tattcaacag gatccagcta atactatttg 18480 gaaatgtcag cttcgaaaag tgtaggggct aaaatggtta atcctgatta tgttcctgaa 18540 tggtatatct cgccttttca acatgtgcag tacacgcttg ctcgaaatca actacacatg 18600 gatttgttat ttgaagatat ggataaggcc gatcaatttt tggatatggg agcggatgcg 18660 caagttagta ctttttctga tggtgcatat gcaatcgtcc aaattggtga tacggcggat 18720 aaagaccgaa ttcaagttta tggattgctt ttacatgaag ctgttcatgt ctggcaaaag 18780 attaaaaagc tcatgggtga acgagaaccg agctctgagt ttgaagctta ttcaattcag 18840 gcgatcgctc aagacctttt taaaatgtat gaagaaagcg aggtgaatga tgggatggaa 18900 ggggaaaaag ccaactgaat ttagttttga tgtgtctaaa gcagcagaag accaggtgaa 18960 gcatattgtt atggatactg tgcaatcctt agttaattta agtcctgttg atactggagc 19020 ataccgtgct tcacatattg tttcggttgg atctgctgat ttcggcgtgc gtgaacctga 19080 aacaaaccct attaacgacg cagcgattca ggcaatgaag attaagttag gtaatttggt 19140 ttatatccag aacaataaag cttatggacc gcgcttagaa aacggctggt ctgatcaagc 19200 accacaaggt atttatggcc tcacgtttaa ctttatttct caaaagtacg gtggctaaaa 19260 tggcaatgac tttagagcag acaaggcaag ctattattga tcgcatgcaa agctttacgg 19320 gtattgctca agaacggatt cagtatccaa atgcacctga ctttactgtt ccaacaaaag 19380 gtgtatggtg ccgtttaacg attgcaggtg gcccgagttt tacctcaggc attgcagata 19440 agccatgtac acgccgtacc ggtaatatca tgatccaatg ctttgaccga ttacatactg 19500 gagagaaggc cgtaacggtt ctaagcgatg cattgctggc tcattttgaa tatttcacaa 19560 tcgaacactt agaatgtttg aatggccaat ctatttatgc gggtaaagat gctgatttca 19620 ttcagtataa tgtgagcatt gggtacaagg tgaattgata tgtcatgtat gctgacttta 19680 gaagaaatcg aaattaaacg gcaagaactg gaacggcatc ttgaagatgt tatgtctgtt 19740 gagttgagca aatggcaatc tgaaaacaag ctatgtgttt ctgatgtgaa tatacgcttg 19800 gctaatgttg ttagtctcgg agggcctaaa cataacgttg ttactggagt aagtgtcgat 19860 ttagataatg agctttgagt tcaagaaaaa gctactgcaa ggcgattatt tttaatgacc 19920 tcagcatatt atcatttgtg attacattct gttacagtaa tggaaattta taacaaatgg 19980 taaaacatga aaaaatcaac tttaggctgg ggtgccgcag gattagtagc tttagggatt 20040 tttggttcag gcaatgataa ctctccaaaa caaacttcag actcagaaaa tgcgcagagt 20100 gcagtagagg aagttatcga atcaaaatat atcaacacta attctttaaa tattagagat 20160 aaaccaaacg gtcaagtagt aggaaagtta ggacgtgggg aaaaagttga tatttatgag 20220 atgaaaggaa actgggcacg tatttcctta aattcctcat cacctcagtg gttatcaaca 20280 aagctattat gtgaaacgga tggctgtttt aaacaaaagt ctcgatcaac cacgtcaaat 20340 aattatcagg ccttaaaatc tcatcctcat cattctgaaa gaaaacagaa aaaaacctac 20400 tacgatagtg attgttcatg tgctgtggtg gattattgcg tgggtcctag aggtgggcac 20460 tactgtatta cgagtggagg aaacaagaga tataaaccta gatattaatt aatttgaatt 20520 atgagacctc cattttgaga ggtactttat gtcttaatca ctaccacctc atcggtggtt 20580 tttttatgtc tataggaatc acttatgagc aattttgtat ttaagcgtgg tgacactttc 20640 aacttaaatc ttcagctagt tgatatggat gaagccctgc aatatccacc agatgatgtg 20700 cgccgtgcaa ttgatcttac aggttatacc ttcacttcac aggttaaagc tctggctgat 20760 ggtgctactg tggccacatt aacttgtact gcattaagcc aaagcacaca gaaagggtgg 20820 ctgaacatta aatctagtgc aagcactgca acttggcctt tagggctgtg tcagatggat 20880 attaaagctg tagttagtgg cactacacag cacactgaaa ctttgacttt ccaagtgatt 20940 gacggagtaa cagcataatg gcaaatcttg tttttaaatt tagttgggat catcggccat 21000 tcccatataa cgcctcacag ggcaagcggc agtttatgtt gccatttgcg tcaggtattc 21060 ccaatctggc acccaacttt tctcaagtag ttggtactgc agctatctct caaggtggaa 21120 cgggggcaac tacagcggct ggtgcacgag ctaacctagg cgcagctgca agtggggtaa 21180 atagtgatat tagtgagctt aagggactta caaccccttt atcaatttct cagggaggat 21240 taggagctga taatgcacag acagctagaa tgaatttggg gttaggaact gctgctgtac 21300 tagcgtcaac aacaagtcaa tatgatccta cgccgggacg agcactaaga gtcggtgatt 21360 gggggatagg ggctgaaggt tctcgtgtat ctgatatggt tgctcctctt aataatggtt 21420 tttttcgaac agatgacact ttaacaaatg atactggtaa tagtattggt ccttatggtt 21480 tctttttaca ctgtacccga cgctcaatgg gtttatatac aaatggaagt cattcatttc 21540 agcttgggaa agctgcctca tattctgccc tgaagtatcg atttaataat agtggtactt 21600 ggtctaattg gtttaattta ttgactgcac aaaatactac aactgatgga aatggtttta 21660 ttaaagccgc ttcaccagtc gttaagcttt tccaaagtca tattgagcta aataacgatg 21720 ctgccaagca accgatcact tttgacaaat taggcactgg tgactatctg attaagggct 21780 ctttaggctt tgcacaggaa ggttggtata tcgaagtacc taaggatgcc aacggtaata 21840 cggtagtagc agttgaatat tcaaccttag aaaatggtga tctttctatt aaaacttata 21900 aacgtaagtt tgatgtggaa aaggcagcca ttgtagctga tctcgaaaat ccacttgata 21960 ttccagaagg ccgctggatt gatatccgtc tgcatgaaga acctgaacca gagcctgaag 22020 agccgttgag tgaaacacca gtggatttcc agccgactaa cttatctcag gcagttgctg 22080 cagccatgaa tggcgtggaa ccgccagaaa tctcagaaac agacgaaaca ctttaataac 22140 ccgcttaaac agcgggtttt ttattgccta aattttggag aaccataaat gagttcaggc 22200 gcaaaaattc gattatatgc ttgtgaggaa gcagttttag gaacaactcc tgcaaatcca 22260 gtctggtaca ccgttcgccg tgttactgat agtttgactg aaaatgttac tactgaagat 22320 agcagtgaag tagttgattc acgttttcgc caaggcgctg ttgtaacgga agccgaagta 22380 actggtcaac tagagtttga attatcacta ggtacctttg acttattctt aaatgttctc 22440 gctttcaata actgggctgc aaatgcttta agttttggtg gtggagtacg taagtctctt 22500 accttggtaa aagtctttga agatattggt caagtcttta tttatcgtgg tattcaagtg 22560 aatacaggtg aaatgacgat ccagaccaca ggcaaaatca ctggtaactt tggtttagta 22620 ggtagctcat ttacgcgaca gcaggttaat cctgttacaa atcctattcc agcatcgact 22680 cgccctctgg tgagtatgcc aaatgttgaa aagctactta ttaatggtca atcaattcag 22740 ggtaaagctt gtctgcagac acttaccatc aactttagta ataatctgga agcgatccgt 22800 tgtatcggtt ctggtaagta cacgcctgag ttttacttag agaaaatgat ggatattggc 22860 gtaaatgcta atttcatgtt ttcagcaaca tctgcttctt ggattgatgc tattaaaacc 22920 cgtgatgtat ttacattgac cttcgatatt acagatacca aaggcagtaa gtactcgttt 22980 aacttcccgc aacttgaagt taaggaagca aatcaccctg atggtggtgg cgatgacatt 23040 attacaatag atatcaattt tgcccaagtg cgtaccagtc caacgattgt acgtgctctt 23100 gtgtaatcaa cttattcagt aacaaagcct atggaaaccc atgggctttt ttatttctaa 23160 aaattagagg ttgttatggc tttaaaagtc ggaattatta aaagctcaga cgtatcaaaa 23220 tggtgtgaat acaagggtgc tgatggcgat gtacaggctg agttcaaagt ccgtggtatc 23280 gcttataagc cttttcaggt agctattgaa cgggcaggaa accagatctc gtctaaaggc 23340 tatgatgtga tggtcaaaga tgaagatgcc aagctttacc acgagctttt aatggatgca 23400 tgcgcggccc acttaatcga agactggaaa ggtgtggtat ttgccgaaat cgtagacggt 23460 aaaactgttg agtccgaaaa gccatataca cctgagaatg cctcaaagct tcttaatctt 23520 ggtgatattg gtatttcaat ctggctattc attaaagaac aggcccagaa gattcaggaa 23580 gacgcagaca aggacaaggc tttaattctg ggaaagtcat ggagctctac aaataccaaa 23640 aaacgtatgc gtcgaaaacg ccgcacgaaa tcgagcaaat caagttctta ggcggccgta 23700 ttccggatcc gccagaatat tcgtatgcgg ctgattcaat tctttcggca tttagcacta 23760 tatgtcgatc cagacgttat gagcaaagca taccgttatc tttagatcag caggctatca 23820 atgtctatgc tgagcataat gatttgccag tggctgctca tatttttaat gactgtattt 23880 ttgcgttgga taatttgttt ttggaggagt gccataagaa gatatcaacc aaaagcaaag 23940 gtaagtgacc aaattaggta ttgccaggga ctgaaaagcc taatttggtc aaaacgtcaa 24000 acaattaagc agttgctctt aaacgcgact caaaataacg cagtcgatgt tacaaaatac 24060 ttgatctgga ttgacagaaa attaccttta aggtgttgcg cgtgattatc aaatgatgaa 24120 taatcacctt accgtcaata tttgacggtt cagcattctt ttactctttc caagaacctt 24180 ggtgtttgct tgtatgtgtt taacattaac tgaagctaaa caaaaactta gagcatttgc 24240 tagagatact agcaaaatca agttaactgc acatgcaaaa gaaagaatga aagaacgctg 24300 tatctctatg aagcaaatta tttgctgttt tgagcatgga gatattactg agggccgtac 24360 ccaaatactc gtggtgattg ccagttaaat gtttctgttc gcactgcagg cgaatacata 24420 acaacagctg ttgcaatcaa gcagagcgag aacggtgaat tctcagtagt agtcactaca 24480 tttagagagt aggctaaatt atgtatcact atgaagaatg cggtctgagc aatatttggc 24540 tgcgcaatgg atttacaatt gaaaatgatg aagactatgg tgaactcgta tctattgaat 24600 ctgttcatga gcttcataat gccattgggt tgttcttaat tacgcaaaag cctgacttga 24660 atggtgagga aattcgtttt ttacgtaaag aactaaactt gtcacagaag aatcttgctg 24720 ggcttttagg agtcagtgag actagtatta gacattggga agctgatcgc ggtttaattg 24780 gtaaacctac tgagctatta cttcgtgcat tatataaaga gcatgttcaa ggtgatggca 24840 aactaagaag tatgattgag tcattaaatc atcaggaacg aactttagta ccaagtgaaa 24900 ttagtttttc atatggaaat aaccattcat ggcatcaaac caattgtgaa atagcttagt 24960 tagttttatt tgatagaaac caccttcggg tggttttcct ttatgtgaca tttagtaacc 25020 agtttgttaa agttagtaca ctttataaca aacggtgaaa ttcatgaaaa aaatattggc 25080 tgcgggttta attggtcttg ggttggtggg gtgcgctact ccagcctata attatcaagc 25140 tatacctaaa aatataagca aaccgccaat tggatcagtt aataaagcat ttgtagggga 25200 tcaaatgctt gaacagggaa tggtggttga tcgtgaagtt ctaaacgtcc ctgaaaatat 25260 taaaattagt tttgcttatt cacttacttc aggcatttac ttaaaaacag gcaaaaatga 25320 aaaagggcaa tattttcagc cattcaacac tgtcagtggt gggggatggt tcagaaaaac 25380 cctttagctg acccatttaa agtagttatg ttagatactg aaggtaagct ctgtgtagta 25440 acagtattta atgcaaaaaa ctgtactgat aaacatcaag ctactatgaa gacagtagca 25500 attgcatcag ataattcctt ccaacaaaca ttaatttata gtggaaaatt tggaaataaa 25560 attaatgtcg ggtaccgtga attctcaagt aatcaagcac gtcctgcatt caataatgat 25620 gttgaatatg atttaagcca atctaagcaa ataggttata aaggtgcttt attggaagta 25680 attgatgcca ctaatcaaga tattacttac aaagttttga agaactttaa caaggtagat 25740 taagatgagt gcaccacaat ataaaccaat gagagaaagt gaagtttgta atgctatcgg 25800 gtgggtgtta atagctcttg gctttatcgc aggtttttta tttattcttg catttggtcg 25860 aattgaagta gcttcttact atggtaaaga aacggtttgg tctggagtta tgatagcaac 25920 aggaatcgga attatattta atggattcct tgcaggctac ttatttcaaa aagtagctag 25980 tattcttcgt taccatgaga ataaataata tcttgcataa gcaccctagg atgcttttta 26040 aaattggttt aactaccctg cttggtaatt atatttaact taaaaagaac tacccactca 26100 ttgagtgggt tttttattgc ctagaggaaa gtaaaatggc acaagaatcc cgtttggtca 26160 ttgttattga ttcgcaaaat gctgaacgta atgcgcgtaa tctaggcaat gaacttgtta 26220 gcattgaacg taaaggtgaa tttgcatcta agtctatgga cagcttgtct gtagccacca 26280 gagctttagc tggacacatg gctggtttat taacagtagg ttcagccatt tcaaagatgg 26340 atacatatac tggattacaa aatcgcctta agttagtcac taacaatcaa gttgaactaa 26400 ataaagcaac ggaagacact ttccgaattg ctcaaaaaac ctattcagct tgggattctg 26460 tgttacaggt ttaccagcgt tttagtgata atgccaaaac tttaaacctc acaatggatg 26520 acacagcacg tttaactgaa acagtttcta aagctgtagc aattagtggt gcaagcgcag 26580 aagctgctga tgcagcttta gttcagttcg ggcaggcctt ggctagtgga acgttgcgtg 26640 gagaagaact taattctgta atggagcaaa ccccagcact agcaaaggct attgctaaag 26700 gtatgggtat tactgtaggt gaattacgtt cagtagcagc tgaaggaaaa attacttcac 26760 aagaaattgt aaaagcgctt agaaatgtag aatctgatgt tgatgctctt tttgctaaaa 26820 cagatatcac aatcgggcag tctctcacac tcctaaacaa cgagatcaca aaatttgttg 26880 gcgaagcagg taagggaagt ggtgcggcac aggtattagc tggatcagtt caaactcttg 26940 caagtaattt agatttaatt gctgatgggg ctttagtagt tggtattgga tatatcactc 27000 gtgcaatttt gatgaagagc gctgctatta aagagggaat ggcttcaact ttagcgagcc 27060 gccaagcatc tgtattaaat gctcaagcag aatatgcaga agctaccgct gctttgaatg 27120 cagcaaaagc tcatctcgcg aatgtgcgag caacaaatgc agaaacccaa gctaaatttg 27180 gcgcaacagc ggcagcaact cgatacgcac aagcacaggc agcagtaact gctgctacaa 27240 atgcacaaac agcagctcaa attaagctaa atactgcaac ttcaattgca gggagactag 27300 ctaaaggggc gtttggatta attggtgggt gggctggagt tgcaacatta ggagtaatgg 27360 gattagcggc agcctattct tattttaata ataaggcaga ggaggcaaag caaaagcttg 27420 ctgaacaagc taaagttgct gagaaagctg atgaggagtt aaaaaaatta actggcaatg 27480 ataaggctaa agcagttaat gatttaacta ctgcttttaa tgcacaaaat aaagcattag 27540 agaaatcatc gcgtgctgta gggtctgcat taattgatat cgagaactat gcacgaggaa 27600 atagggaggt tgaaaaaatt tcccaagaag cgagaactgg aactatcagc tatacagaag 27660 ccattgaacg tctaaataaa attaagttgc ctacagatct atatgaaaat ctgaaaaaac 27720 aggctgcgca gtatgatgac aatgcatcta aagcaagttt atcagctgag aaacttaaat 27780 tattaagagt tgaagtgaaa cttggaggta atgaagcaca aaatgcggca attcagcatc 27840 aaaaacaagc ggatgcttta ggaaatactg ctactgaagc agaaaaggca actaaggctt 27900 tgcaagatta tcaagccaag caaaaagata gcgttattga ttcaatctat aaatcaggtt 27960 ggcttgataa aggttacact gttgctcaag ctaatgccat tttagaactg caaaaagcta 28020 aaggaatgag tgcaattttg tctaaagatg aaattgatag cgcacttaga aatctcaaga 28080 tcatcgaaga acaacaggag cgagaagata aattaactga agctaaaaga aagcagacgc 28140 aggaaattga aaaacaagca aaacttacta aacgcttggt cggtatttcc ggtcaatccg 28200 gtattggtac tggtccacat cttgacgtcc gatatggtgg ctcattgtca ggtcagaaag 28260 tttctaatga acatctggct cgattacagg cgggaggaaa acctttaact tcctacaaga 28320 tcagttctaa ttatggtcca cgaaaagccc caactaaagg ggcttcttca tttcataaag 28380 gtattgattt ttcaatgcct gaaggaacac caatcacgac caatgttgct gtgaaagata 28440 tcaagacatg gtatgacagc aagggaggtg gttatgtcag tgaagtgatc tttgaggatg 28500 gagtgtctct taagcttcta catcaatctc ccaagatgca gagcaaggtg aaaggtggtg 28560 caagtaaagg aagtgataaa gcagctggtg atattcaatc tcaacttgaa cgtcaacagg 28620 atttgcaacg gtcacttgaa aatgaggtgg ctagtgaagt cggacggatt aacaataata 28680 gaaaggcaag actggaggat gttgataaag caaactttag cccggaacgt actgcagaaa 28740 taaaggcgga aataaatcgt cgtgcagata atgatattgc tatagccaaa caagccctta 28800 gaacgaaatt ggaagactat aaggagttcc agaaaaccga ggaacagtta ctagaagagt 28860 cctttaaccg taaaaagttc aatgcagctc atgaccttga attaagtaag tttgagcaga 28920 agcaagctgt tgaattgctg gaacagcaaa aacagcaaga gttagggtta ttaaaactag 28980 ctcaggaaca gcggttgttt caagcccgtt tatctctgct ttctgaaacg caagccatgc 29040 aggaacgtta cagactcgaa cgggaggaaa ttcttaagaa taccaagctt tctatagaag 29100 agcggcaaaa gctaatcgca ttatctaaag ccaatcagga taaagagaca cgcgataaag 29160 tgaataatgc tgttcaaaac tggggtggta tccaagcgga tatgaatggt accggagaat 29220 ttttcagaca ggatcaggaa cgatttagcc gtttaaatgc tgcaaatgat ttagcagata 29280 gtcaatttgc tgctactgat cttgatgaaa aaaatggttt agatgttcta aatgcacaca 29340 tggaagcagg actcatcaag caacaggact tcgaaaaccg gaaaacagct atcattcaag 29400 ctgctcagga ccaacgcaat cagatcgctg ccgaatatgc tcagaatgct caggatattg 29460 aagataagta tcaccaagat cgattgaatg ctcaaattgc tcttggtggc caaatgatgg 29520 gttcactcac atcgatgttt ggttcaatgt ttggcgagca atcaaaagca tacaagatca 29580 tgtttgctgc tgataaagct tatgccattg cagctgcagg tatttctatt cagcaaagta 29640 ttgcaaaggc ggctagtgtt ggttttccag caaatatccc attaattgca agtgctattg 29700 cacaaggtgc aagcatcatt gcaaacatcc gggcaattaa agatcaaggc tttgctgacg 29760 gtggttacac tggatcaggt gggaaatatc agcctgctgg tattgtccat aaaggagagg 29820 tggtctggtc ccaagaagat attaaacgct ggggcggtgt tggcttagtc gagaaaatgc 29880 gtaagagtgc aaaccctgaa gcttttctca ataacaatgc cttggcagat agtgtcatgc 29940 gccgtgcaat gatgagttct aatgccttta tagaaagcca aaagcaggct gacatcttta 30000 atcaaccagt tcaagatact cagattatct ataagggtaa tagagacaca cctaagttag 30060 cttcttcggc aaattctgac ctgttccatg atggcaaggt ctacttctca tccaatggta 30120 tagttcagga tcgctcaaat ctggatgatg ttcaggactt tactttagga cgtacttcac 30180 gccctcaagc tgagattatg ccttcaattg agcaatcctc tccaactatc aatttcaaga 30240 ttgaagtcgt gaatcaagtc agtggtgcaa ctgttgaagc tgaacaattg gatgagaaaa 30300 ctgtccggat cattgttaca gatgaactgg ataagcagct tccaagaaag gtaccgaaac 30360 ttgtaagtga ccaaatcgca aatccaaact caaccattag tcggtctttg actgagaata 30420 cgacagcaag acggaatcgt taatttaaaa gctaccttta gaggtagctt ttttaaataa 30480 attaggacaa aatttcaaaa aattggtgaa tattcttatg cttcctccag ttcctaaaac 30540 taagtcatca gaagtaaccg atattattaa ctctgctgtt cttactggat cgataagtga 30600 atttcagtat tttagatgca aacggttgct taatgatatt aaagaaactg agccactaga 30660 ttggttttta ttaagcaaca gtattattga aatgtatttt gataatccta ttcttgcgca 30720 tcaatacgct cgagaagtac tgaaaattag caatagtgta tcaattttat cgaatcttta 30780 ttttgttttt cttagctcag tagatttttc tagtgctaat gaaaatattg ataaaattat 30840 aagtttgtgt agtaaacaaa atttaccctt agaaagtttt attcctattg acttcaaacc 30900 tataacttat tttctagatg gaattttaaa tgatgattta aattattata aaagatttaa 30960 aaaggaagac tttaatgaat ttattcagtt ttttgaaatt aaaaataaac tagaaattga 31020 ttctagagtc ttgaaacata tcggttcaat tctttttaaa tgttttaact caaggaatgt 31080 tcggtgtcga aaatatgaat atagttttat tgatgatgaa tttttaatat tgctttatgt 31140 cgatagaagt tttgatgaga ttgacgctat gaattcagaa atatttagta aatgctatga 31200 tgagggttta attgatgaac tgaataaact ttcatatttt attattcctt atgaagtggg 31260 cgtggattga aaaatggcta ctacagatac actaaattac tgttatgagc tgttaggtaa 31320 ttctacaaaa tatgatgaat gtcacaaaag gaatattata gggcgtgctt attaccatgc 31380 tttttatgaa gtccgacatc atttagaaca acgactatta tggccagtaa caaagacaaa 31440 atgtggagct catgaaaaag tctatagcag acttagtggg taccctgcgg gttcaacgtc 31500 tgaaatgatt cagaaaagag ctgcggaaat caaaaatcga atacaaaaat taaagaggtt 31560 tagaacaaca gctgactatc atcttcatct aacgatttca aatcaattaa taaactatat 31620 tttacatgaa tctagtcaga tatctgaaga aatatcaaga ctttagttgt taaagatact 31680 tttataccga cccattatga ggtcggtttt ttattacctg aaggaaagtt atgtacaagt 31740 taaagctaaa tcctcagacc agcggctatg gcgtaacacc aggtgatgat gtgaaacgtc 31800 agcagatgga tggcggacgt ggtcgctatt acatcgatgt aaaacgtaat agtcatattg 31860 tcgatgtgaa ctggaattta agtaaaaccg atttcaataa aatgatggct ttctggcgga 31920 tctatcagaa taagccagct tcattctatg cggatttggt catagaccaa ggaacacgtc 31980 agcaatacca atgcaatttc attccgaact cgttcaagac caatgaagtg aatggcaacc 32040 tttaccgggt aaatgcacag ctcgaagttg ttcaaaacca gcctaacctt acggccgata 32100 tagctttgat taaggattgg gaggtctaat ggataacgaa tacgccaagt tctttttcaa 32160 tcgtaaagtc gatgtttatc aactggaatg tattgagctt tctcatcctt cctttatgaa 32220 catataccga atagttcgta atgatgatcg tggggtgtat gtacaacata aggaaggatc 32280 cggtcaggtc tattatgaat ttttgccagc atctattcaa agatccggaa tgctgggtga 32340 tctggaccag acattaaccg tttctatctc tggtctaggt gatgtgatgc cagatgagtt 32400 tgaacgggta atcgaagggc aatatccaga tgtaaagcca accgtaaatt accggattta 32460 cagttcagac aatctgaact ctccaatgtt ttatttactt ggactgcaac tctccagtgt 32520 tgccatgaac cataaagctg tgacgttcaa agctgaatct ccacgattaa ataccgctaa 32580 aactggagat atctttgcac tagaccgctt tactggtctc aagggggcta tatgaaaagt 32640 catgatcatt tgcttgatag acaatatgac gaggaaaact acaactgtgt tcattttgct 32700 catgaagctg cattggatct atatggaata gaccgggtgg aagcacttga attttttatg 32760 aagcctatta aagaaaaggt atttctacca tcaaggttaa aacttttaaa tccactgccc 32820 atgcccaagg aaggctgcat agtcgccttt cactcgagat accgaaacaa gcccccacat 32880 gtggggcttt ttcgtttggg gcgtattttg catttgcagg aatcaggcgt ttcatggatg 32940 ccaattcaag tcgttcaagc atttggattt aatcgtgtga gtttctatga ttaagattat 33000 ttataaacaa gaccctttat ccgaagacaa aacaattgaa cacgccgaaa ctttgggtca 33060 atggcttact tcaaaatatg attatatgcc tgaacatgtc cgtattttcc atacaacaag 33120 taatatggat catgccgaaa tttcatttgc gaatgaagtc acaccgaaga atgcatatga 33180 attaaagcag ctcgatttct tgccaggcac tttcattgta attgagaatc ccaagggtat 33240 agaccccata actctagctt ggatagcggt tgcttctata gttatgggtg tggctgttgc 33300 attattaatg cctgtgccct caattaccca aaccaaccag aataacaatc aatcctcgtc 33360 tgcaaataac gaattatcaa accgtgaaaa taaaactcgc gtaaatggtc gtatcgcaga 33420 tatttatggt gccgctcacg atacccctga tctgattact gtgccttaca aggtatatga 33480 aaacaatgtc gaagtagagc atgttgttgg ttgtattggt cgtggtcact ataaaattaa 33540 cggtgcatat gatggtgaaa ccaacattgt tgatattgcc ggcgcatcgg tagaagtctt 33600 tcgaccaggt gtagatattg tttcaggtga gccatatttt tcgcttggta ccgaaattac 33660 cacgccgcca ctaacggttc agcatcaaac ttctgttaat ggccaagttc tccgtccagc 33720 agatacacag tctttagaag gtacgaacta ccttcatttt gcatatccaa acgagatcct 33780 tcgggcatct gcaaacaata cggatttaac cactaagttt gtaagtaatg accgcgtaga 33840 aatcaccaat gcctcattca cgtttaacgg ccagacttat gatttaaacg gcacttacag 33900 cgttctatcg gtagcagatg atcgaatgac gttatcaaat ccggcggccg ttaatgctaa 33960 ctggttaaag cttaaagagt taaataacca acaaactgca gctttgtcac caaagatcag 34020 ttcaataggt gaaaaatgga ttggtccatt cattctggac aatgttgaac gtagccgggt 34080 gctgtgtaat tttgtggcca caaatggact ttataccgtt tcttcaggtg ggaatcaggc 34140 cgctgttaac gtcacgattg aagttgaagt aacaccggta aatgaatctg gtgcagcgat 34200 tggtaatccg atgctgaagc agatcatttt gaaaggttcg gcaaagtcgc gtcaaaccgt 34260 tggcgcaacg ctggatatgg tgacatttca aggtcgctgt agtgtccgtg cacgtcgttt 34320 aacaccaaca ccggcggtta caacggtagt agatgaagta aagtggcagg cgctttatgg 34380 tgcttatcct ttgcaaagca cagtgtatga acatgaaacg gtttttcgtg cgcgcactta 34440 tgcaaccact ggagctttat ctgttaagtc ccgcaagatc aattttgatc ttcagcggat 34500 gttaccgact tttaaaaacg gcgcaatgac gacagagcta tttccaacat caagctttgc 34560 tgatgcattg gtttcaatgg cactggatga caagataggc cgccgtacga tcgacgaaat 34620 agatctggaa aatatctatc ggacttataa cgatgtagtt gattattttg gtacaccact 34680 tgcggctgag ttctgtacta cgattgatga tacaaacctg tcttttgaag agctggtcac 34740 caatctttgt gatgccgtgt tttgtactgc atatcgtcaa aataataagc tcaagcttta 34800 ttttgaacgt ccaactgata actcggtaat gctatttaac ttcaggaata ttattcctga 34860 tagttacaag catgatctta cctttggcgt gatggatgac tacgatggac tgatctatga 34920 atacacggat ccggccgacg atagtcgtat caatatctat ctaccggata aaggggccaa 34980 gaaccccaaa gaggtgaaat ctgtaggtgt gcgtaacaag tggcaagctc attttaatgc 35040 gtaccggctt tggaacaagc ttcgcttcca gcgcaaatcc attacctttg atgcggcacc 35100 tgagtcagaa ttactggttt tacgtgaccg gatcgctgta gctgattatc gcaatggtat 35160 tcatcaaagc ggtgaggtgg tacagcaaga aggtttaatt ctcaccctaa gccatgatgt 35220 cgatttcatt gcaggcaaga gttatgtgat ttatttgcaa atgggggatg gtaccgtgga 35280 cctgattccc gttacgccgg gttcagccaa gaacaaagta gttttagggc gtttaccgaa 35340 cggggcctta aagcttagtc ccgatgactt tgtgaatact atctacaccg tagttaatga 35400 cgataccaaa ggctcactgc cttatctggt tgcaaaaaga gaaccggctg accagttctc 35460 taataccatt actgcaatta attacgatga acgttattac ctcaatgaca aggactttat 35520 tgatgtgccg gttgatgatt caccgattta cattcgatat gaccagctgg atattaatct 35580 ggcacgttta tatcagatgc aaagagggga tttgccaacg actggagaaa tcagttttgt 35640 agttgaagca ggtgcactag tttcaagttc aagttcttat cgaccggaaa ccagatttgt 35700 ctataaattc gactataact ctagtcctgc aaaacgagag tatatcgttc cagctgcatc 35760 agaattacct gctattgata ctggtgagtt cccacctgat ctcgtggtaa atttgactat 35820 taaaggtgct gttgttggac gtggtggaga tggcgggttg ccacatttgg catttggtgc 35880 atggtctacc gatccggatt ataactttac taaaacccgc cgtgacggtt ttcagggagc 35940 acccggttta ttaaaccggc acagtaaact aaacctgatt attgatggtg gaactctggc 36000 tcgaggcggc tcaggtggtg gagcaacacc aagcggtatt tatacaggat tatcgtatgg 36060 agttcagggt attcccggtg gagctggagc accttttggt cgggttatga ccggacaacc 36120 tattactaac gattcacaag actggcgttg gtacttaaat ggtgacttta tggttgtcaa 36180 agtaaccgat gccgaagctt cggtacccgg taaaggttac cgaacccaaa atgatcgata 36240 tggatctcca ttgtctggtg atggtggagg ttggggccag cgcggtacca agtccaccaa 36300 tgatggaaca tggaactggc aataccatgg cacaactgaa ggccagccgg ggccgggtgg 36360 acctgcaatt gttggggtgg caccacttac aactcaattg atcaatggag ggaaaattct 36420 acaaacactt taaatcttaa aagaactttg agcacccaat tcgggtgctt ttttattgcc 36480 taaattttct ggagatataa atggaaccag tttcaacaag cggtttaaca gcaattttaa 36540 aattttatgg tgcagcaatt atggtgacgt tagcggttgc tttagttgca gcagttgtat 36600 tgatgacacg tatgccacga tcacctcaag aatgggctgt aggacttatt tgtacggttg 36660 tatcaagttt ggctggcggc tcattcatta ttgtgaagtg ggggcttcat gaatgggtta 36720 ctgatgtatg gggaatgatt gcacttggtg ggttcttctt tgtttgtggt ttacctggtt 36780 gggctttagt ccgttggatc tttaatttca tagataaaca ggaagggaaa acgattgttg 36840 aagtgattaa agagtttaag aaagccagaa aagacattga aaacagctaa tgccgccttc 36900 gggcggtctt gtttagaagt acacgtataa gagagaaatt acctgttgac actgcaagcc 36960 gctgactact acgaaaacct attgacgacc aatattatga aacgaccacc ttcgggtggt 37020 tttcctttat gtgacattta gtaaccagtt tgttaaagtt attatattta taacaattgg 37080 tgaaattcat gaaaaagata attttaggga gcatgttagt ggctgttttt tccacatcat 37140 tttcacatgc tttagctccc aaaaatggag atgagccaac ttattgtgag cagattgttt 37200 cggtccatgg tttattaact agagcacaat ttgaatgtgg atatagtgaa tataacaatg 37260 agttaatctc agattcagcc aagtgttttc agcatgaact tggcgaagaa tatggaaaaa 37320 aagtccttat atttggcatg aaagaatttg accgaaatgt aaagaaagac gggaagaata 37380 agatttgtaa tagtttatta aaagaatttc cagagtatgt aaggaagtaa ctgatgaaaa 37440 agctactacc aattgcattt ttactcacag catcatttgt aactcactca gccgatacta 37500 atgataaaca ctgtagagat gtgaataaac ttgctgaaaa tgtcatgctc tttaggcagg 37560 aaggggtttc tgtggttaga caaatggaga tgatagagag tatcaaacca agcagggatt 37620 tcaaaaggtt aatggagatg atggtcgagg aagcctataa agaaccaaag tttggatcag 37680 aagagtataa ggcggaagca ataactgaat ttgcaaacaa ttggtacatt cagtgcaagc 37740 aagcaaatcg aaataaatag agcactttaa ggtgctctaa ttattgaaat tgagaaaagt 37800 ttataagtag gtttttatga gaaagattat tttattgggt cttatttgcc ttcctgtatt 37860 cgcatatgca aatagttgcg aggtggcaaa aagtaaaatt aatataagcg gtttagcatt 37920 gggtaaatcc attttatcac tgaaagcaga acatcctaaa aatttgagca tagatcatga 37980 gactaataag gcaaatatta actatgttca ctctaatgaa tttgaggatg cttttagtgg 38040 aacaccagct accaatgcgg gattcatttc ttttgatggg aatacaaagt taattaatgc 38100 gtttagcgtt agttttggtc acttagataa ttttagtgct aataattata aaaatgggtt 38160 agtggcgttg tattcactac caaaaacagg gtggatagaa tcaaagagta atggggttaa 38220 agtttttaaa tatgaatgta cggattattc tttagagatt aattacaatc cagaaagaag 38280 tagttttatg atttttaagg aaatttagtt ttatgttctt aagcacccta gggtgctttt 38340 ttaatgtctg atttttctga aacagtaatg gtgtaacctt tcatacggct agctaactcc 38400 atcattagag tttcggtagg gatcagttct acagtttttt cataattttg attttcaaag 38460 cttttttcaa gacgggcaac aatgtcggca ttcattgatc gactgtttaa ctttgctgat 38520 tcaagtattt tttcttttag ttcttgcgtc atacgcattt tgtattcaac gtctgagctt 38580 ctagccatgg tcctatactc gaataaattt tatttataat aatatcccca atggggattg 38640 acaagaagtt tttaaagtct taaattgtaa aagtccccat tggggatgta aaaagccccc 38700 aactttctga cggcaagggg cttttatcaa caaccatagg aaaggatatt gatatgtcta 38760 gtttagcatt aagttttaat gaagtgaaat tcaatcccgt gccacggcaa gatggccaga 38820 tttggctttc ttcaggtgaa ttggcacaag cattaggata taaacaagag aacgcggtca 38880 gtaaaatttt taatcgtaat tctgatgaat ttacggaaaa tatgacacaa attattgata 38940 atcctcggct acccaatttg ggtatgcgga tcttctcact acgtggctgc cacctaatag 39000 caatatttgc tcgtactgct gtagcgaagc aattccgcaa gtgggtactt gatgttttag 39060 ataaagaagt tggcacacca gttgccaaaa cccacaaatc cgaacgtgaa cccctaacca 39120 atgctgtaaa tcttcttgta gctaaaacta agcatttgaa ttacagcgat gcttataaat 39180 tagttcatca gcgtttcaat gttcagcata ttgatgaaat cccatatgac atgattcctg 39240 ttgcagtgga atatgttcat catctgattg cgatgtacag tagtgcagag aagaaggctc 39300 aaggttcttt atttgataat gaaacattgg gtttggttaa ggatctggta gatgcaatta 39360 tttcccaaaa ctttgtgaca agcaaaatct atcgtgcaat acacatgctt agtaatgaac 39420 aaggtcacta cttagctgaa tatgcgttta aaaccaatat tgcagttcta aaactcactc 39480 gaacaatgga tttaagagga cctcttaata gagaaatcat tagtgatgat ttaaaaacca 39540 taagctacac aacaggtaat caacattatg gcgaccgttg gtttcaccca ctgatggagt 39600 caagtcgatt gatgggagta cttgaaattt caggtagtct gattcgtcac taataaaatc 39660 aacttaacaa aacccactca tcgagtgggt tttttaatac ccaaaacaaa accccagtag 39720 cgctaactac cggggttttt cattccaccc accgacgaaa gtaagaggaa agtaaatcta 39780 tatggagcat tttaaaccaa tagtggagct tataaaagtg tctattgaaa agtatggctt 39840 atggcaaaca atagttgcat ttattctttt gttttccgtg ccaatcttaa tgtggaagtt 39900 ggatgtaatt attgcttcta taaaagcatg aaccaacttg aaaaaactgc gccaccttcg 39960 ggtggctttt ttacgtctaa aggaaagtga aatgaacatc gaacaatatc ttgatgagtt 40020 gatcaaacga gaaggcgggt acgtaaataa cccagcagac cgtggtggtg caactaagta 40080 tggaattact gaagcagttg ctcgagcaaa tggattcaaa ggtaatatgc gagatttacc 40140 tctggatgtg gccaaagcaa tttaccgcaa aaactattgg acagctccgc gatttgacca 40200 agtaaataca atcagctcag cagtggccga agagcttcta gacactggtg tgaattgcgg 40260 taccggcttt gcaaaacctc ttttacaacg agctttgaat ctcctaaata acaatggtaa 40320 agcagggtgg ccagatttat cagtagatgg gatatatggt ccggcaactc ttaatgcact 40380 caaaacttat ttggtcaaac gcgggaaaga aggagaaaaa gttttagttc gagttctgaa 40440 tattatgcaa ggtcagcgtt acattgaaat ctgtgagcgc aatccaagcc aagaacaatt 40500 tttctatggc tggattgcta atcgagtatc aatgtgaagt acctaatttt actgtgcatt 40560 ctactcaaga ctgcacagtt acttcgacgt atagtgaggt agttgtaaaa gtttataggt 40620 aagttatagg attgattggt aataatcttt aaattttagg gggggattgt tcagatgtag 40680 tgtattctgt aaaataaaac ttaattatat tttgctttca atacaatgaa cgatcaagtt 40740 ttccaattac aaattgttat aaatggaggt ttaaccccca ttcaatctaa gccagaaaca 40800 cttgataaat tagtaaaaga atttgctata aatcatttgt tgtttccaaa agaaataact 40860 gaacaattaa ttgagattaa ttctcaggat ggttctcaaa caaaaaagat aactaaattt 40920 attgatttgg ttagtagcaa tcaaaaatgt acctaccaaa ttagaaatga ttcacttgta 40980 tttttaaatt cctttgagaa aatagaggaa ttagaaagta tatttgaaaa gttttttaaa 41040 tcgttttcgg atctgacccc atatataaac tataaacaat caaaaagatt ggggctagtt 41100 cttattaggg aggattataa tgaggttaca ttacgcgagt attgtacttc agaagaatta 41160 gatcgaaatg ttattgagaa tagatcgaga aaagttactc gttttgctat ggcggaacta 41220 aatgaaatgg taaatttatc tgtttcaaaa gattatgtaa ctcatgaatc aggagtttct 41280 cgaaatactc tagcaagtgt ttatgatgtt aatacgttat ctactaagga tgtttttaga 41340 tttacaagta aggatgtagt aaagtttata aatgcttcaa agaaatttat tttagaatca 41400 atgtaagatt ttttatatga actattcaaa taatttatct aataatacta tgtatgaaag 41460 tggtaatcat aaaaagataa tcactaagta cgagattgaa acaataaaaa cggtttggca 41520 aaccgataag atacagtctt ttttaaaaga ttacttaaat acagacgtag ttagtattac 41580 tgaccccact gttatagaac aaaagatagg ggaagagagc ttaaagcaaa ttaaaagaga 41640 atttgatatt tttaagaata aatttgataa ttttctgaga tatgaagatg tgccagtcga 41700 ttatgtttca cctattgaaa atgaattaat caacttttat aaacatagta aagttgaagt 41760 tcaagaacaa attagccaat ggattattga ttcttttgat aacacaaagg ttcttcttaa 41820 tattttaaaa attttaggta atattgctcc tgattttatt gatcatcaat ttttaactaa 41880 ttttcttatc gttcttaatc ataaagatac tgaaatcaaa gaatatgcat taagaattca 41940 agagaaatta atgcttccat catataataa tgtactgaag cactctaagt taactccaaa 42000 atggattgat gactatagaa aagaattggt tgaattgtat gaagaagata ataaaggtag 42060 ttaatttaat atgactattt ttgtaagaaa gataagtaaa gcaaaatggc cttctgaaga 42120 ggaaattgca gaaaaagcac tggattcaga aattatacct tttgtcagag ccgatgcctt 42180 aactacttgt ttaaaaactt ctcaaaatac tttatctgtt tgggcagaaa gattaaaaga 42240 aataaaggcg gtttaaccgc cttaaactgt tgcaagctgt gctgtattaa gcacagtctt 42300 gctttgctca tatttcaaaa cgtctttctt tttatatgaa acacgtctcc caattttcga 42360 gaaaggcagt gatgattgat cacaacgcat tctagctaat gtccaaggcg agcaatctaa 42420 ataaagtgct acaacctctt gaggaaactt ctgttcttca ttagccatta tgaagcgatc 42480 caaatattct tgttgctctg catcagatag atttctcaga tcttttaaca tttactcctc 42540 cttactttcc gctttaactt ctcgatcaag acgctcaatc tccgcaatta ataaagcggc 42600 tgcacgaacc aaatcttgtc ttgggctttt tggtttccag aaagtatcag caaaaggcca 42660 ccatcctgca gcatcctcat ccatatacac ttcggggccg aaatctttgc tgttataaac 42720 ccaacctcta cttactacat gatcaacgta acaagatgca gctcgtggca attcatttgc 42780 ttcatagaga ttatcaaatt caggtaaata cccttctttt ataatttggc gttctctttc 42840 attaagaaca tctttgacag cttgactgct atataattca ctcatccctc agctcccgat 42900 tcgcttgcca cttcaaccat caaagaataa ataggcgtgt aatgatcacg agtcccatcg 42960 ccccaatgga atccactatt ttctaaacag ctaacaacgc tgtctggaat ctctttcggt 43020 accaaacagt aaccctctgg caccgcctga gctttggctt tttctagctc tgcatcacga 43080 tgctttgcac atctaagcca agcatcccaa cgtctattca tgttgcttat ttctttctga 43140 gcaatttcag aaggattgtt tgatctagtc ataaacagtt catgctcatt actaaaaata 43200 atgtctcttc ttcctttgta atattggaag gtattcagaa aagcctctct ttccttattc 43260 aaatcaaaca tcattaggcc ctcaaatatt cttctttagt ccactcaaca aactctttat 43320 aaagctgctg cgcgggttta tttaaccggt tgtgatagtc gatcgttatg cggcgccaag 43380 cgactggtac cgcataatgt ttggttagaa acattgcttg atccatgcct tgccggacta 43440 ttacgtagcc cagcaattgc aagtagtaca taaaaccaag catgtgtttt tggctcactt 43500 tcttgtactg atctttcatg ttagaaaccg tccactaata aataatcagg gggagattct 43560 tgttgagtag gtgtaggatt ctctaattca tagcggcgtt ttctcacata ccccattagc 43620 ttcggttgaa tctgcggatc tcgtgcagcc acgtctattt ccaaagcatc tagcgttgta 43680 aggtctggtg cagtttggat ttgaaccatt aaagagggtg gctcattagc agatgccttt 43740 tctttttcta gctcttcaag acgtttgtga gtggcgagaa ggataggctt catttgttcg 43800 tcatcccatg tgcgggtata acgataaacc gcatttactt ctgcaggtgt ttttgactct 43860 tttacacgct gtagaagagc atctaatgcc ttctgatatt caggatctac tttaggctcg 43920 ttagtttctg gaactaacag atcctcagat gtggtgacat ttgtttgttc ggtaataaca 43980 attgttggtt gagtttctgc agaaataacg tcgctaggct tttctgcttt tgattttttg 44040 cctcgctgtt ttttgggctc ttcaccaagg cgaacaacgc taagatcatt attaatttca 44100 aaaccgagtg cttttgaaaa tgcttttaat tgaagcttgg cattttctgc atcacgttga 44160 acaaatcctc ttttaatagc ttcaatgagt gcagcagttt caaatcctat gatataaatt 44220 gaagggagat atgtattgat aacaaaaaca tcctgacccg ctttatactc atcaatagtt 44280 aatggctttg tgaatgtaat gccagccagc tcaatagttt cgattttgat gcagaattca 44340 aaacccggtt taccaaaaac agaagcgggg aattgatcta agtcagaaaa gtccaacatt 44400 tctccaatag gacgacatag aacagtttta cctttttgaa gtgctgcaaa tgcttcttga 44460 gcagttaaaa tatttttcat gctgtcatcc ccgttttcgc taaggtttca atttcttgtt 44520 taactgcctt aagttttgcc gcttcaattt ggataagggc atcgatacct aagtgctcac 44580 aaactgtttt tacatcgagg ccacgttcag caataaagtt ttgaagttca tctctttgtt 44640 gatctgagat accgttaaat tcaggtggac taatccaagt gccacgttgt ttatcaaacg 44700 tgcaattcaa tgctttagct ctcattaaca ttgcttgtcg catgttctgg taatacatgt 44760 gttctttatc aagcgactca gttaattgat taaggtcacc tgcatgctct gcttcctcac 44820 agctttgttt ccagttttct agctcttctt gggctttagc tgctgcaagt tgtgcaggcg 44880 ttaaggtgtt aatgtgatct ttagcttgag taatcaggtc agccaagaaa gtagggtgtg 44940 ctttaagatc aggtacccat acttcaccgg tttcaccgcc taaagcacct gagtttttcg 45000 catgatgtgt aggcgaaggt ttgaaattaa taacgcgggc atttttacct tcaccagtag 45060 taacagttgt tagataaccc atgacatctg cgatacggta aagctcgtta cggtttttac 45120 cacctagatc tgggcggtaa ataatttgat caccgttttg atcttctgat gcgtgtgcaa 45180 tgaaaacaac atctttacct aaactgatca aagtattgat gtattgcttg aacgtttggt 45240 tcgctaaacc ttgagccttt aactttaaag agccatcttt ttgacggtta ttagcagtta 45300 gcaatagatg ggttttaatg cattcaagca ttgcacccac ggtatcaatg actacggttt 45360 tatatggtgc taagtcctgc ggagtaaggt ttgcaacatc actccattgt tgaacctgta 45420 caaccgcacc acgacgtaat tcaccagtac ggtgagcacc acggtcaaag tcaaaagaaa 45480 ttgctttttc cgcagtaaag cccatcgatg atttacctaa acccggatca gcgtataggt 45540 acacaataat tgcttgaacc aataaagttt ggtcagcagt aataatcggt aacgccattt 45600 ttcttatcct tatcttgagc cagtgaagcc gcgcttagtt ttataagcct tgcggtcata 45660 agtagggatg tttgtttcac gcagttttat tgcgagctgc tttctgcgtt ggaaatcaat 45720 ttcttgtgtg agttcattcc aaacttttgg atagtcagtt tggaacctga acacatttaa 45780 aggcgtctta aatccgtctt taactttgta aagaactgag ccattagcat tagatgcgta 45840 cacttgccag ccgatgcgga cagagtagag gcccttatca tcacggccta aaaatgactt 45900 gtagccgtca gggtgttttt tgaaattagt catctttaag cctgcaccaa cttgttacgt 45960 tcgatgaagc cttttagaag gccattgatg tttcggatgt cttcaaattc ggtgaaatcg 46020 ttatatgact taccattaac atcagtgatt tcatttactg tgagttgtgt aatatcaaca 46080 gcagtgaatt cagaacccgg aacgccgtag ctgtctggat gagcttcaaa atcaaagcta 46140 acgtttaaac ggaagctatc taatttaatt acagcaacgc cagaatgttt acctgtgatt 46200 ttcgcggtta acacaccgta agtacttggt tgagttttag gtgtaaaaag agtaggtgct 46260 tcttttgttt ggaaagctgg ctgcaattgg caagcaacta aagaacctcc agagattgca 46320 agagcagcca tgctgacaaa tgcaaatgag ttgaaagggg tagcttttac gttcataatt 46380 gatctcgcag tttgcaaaag cacatcagat ttagcggtcg gtgtgctttt ttgttgtcta 46440 cgagacaaat atagttttat ctattattag tgtcaatagg tataactata aaatgatagg 46500 tgtaaatttc taaattgata ggtaaatcta taagtaaaaa gcccacctta taggtgggca 46560 aatgtataaa attgtttcta taggtgaatt tattaaccag ctcgccaaaa ttgacgaccc 46620 attaccttaa aattttttcc attctcttca gtaacttttc gatctctaaa cttttcattg 46680 agactatgaa gaattagaga cccatcagcc tctttaaaaa tttgcttcaa cattccttca 46740 ccagcgaagt aaacagcata tatttcaccg tcaataactt cagtttgaga aatatcaata 46800 ccaactaagt caccatcttt gatgtaatcc atcatgctat cacctttagc tttgatgata 46860 cgcatgcaat cctcagagac acgtttttct ttaaaaaatg atggtggaaa agggattgct 46920 ccattgattg catcaaaatg aaattcaata gattcacctg gtccacatga aaaattagct 46980 tccactacgt caatccaaat gtaattgtct attcctttat gaaccacttt aggttttaca 47040 aaatcatctt cggaaaaacc atcttcagtt tttccagtag ttagccaata cgcatctaca 47100 cctaagtatt ttgcaatcag tggaaggaaa gcagacttca agctcttacc actttctaag 47160 gcttgatatg tgggttgttt aataccaact gcttcagcta cttcatactg acttttacca 47220 gctttagtac gagcttcttt gagtcgagtt gccaaatcag acataacaaa aaccttaatt 47280 tgtttggaat tggattttat aggctcaact ataaaacttc aaaaaggcat acctattgac 47340 atatgatagg tataactata aattaactat caaaatagga gtttgatagt tatgagccaa 47400 aatctcatcg caatctatga aaaattagtt gagttcttag gtggccaaac aaagacagct 47460 caagtcctac ttataaaaca accctctgta aatgcatggc ttaaaggaaa aaaccaaatg 47520 tccgaaaaga ttgcattacg cgctgagcgt gtaactaaag gtcgttttaa agcttatgag 47580 ctttgtccat cactttcaga attcgaaaca ttaaccgctt aggaccttac ccatgagcaa 47640 agtatcaaat gaattgcctg caagcgctag caataacgaa tcgctcatat tgcaagcact 47700 taacgctagc aaccaaagac aagtagcaga gaaggtggga atagatgcaa gcaccttgtc 47760 gagaatgaaa aatgacaaga aaaacaatgg attgacagag attgaattta ttagcttttt 47820 gttgacagcc attggtttga aggttgtgca agagagtgat gtgtattgct cacctgaaat 47880 tgcagaagca acgcgagttt atttagcaca tgcattcact tcacctgaat acatgcggat 47940 tttattcaaa taaaaaacca ctacctgcgg gaacaggagt ggttaggcat tcaaatgagg 48000 tggatcaaat gaacacaaac aatttatcag aacaaccaat cgaactcaac tcaccagatt 48060 ttttaatagg tgacgttgta gtgcttacta aagagtgccg tactttcaaa tcaaatgatt 48120 tgtttgaagt taaaaataaa actttgacta gtttatggac tatcaaatcg gagaatcatt 48180 tgattctggt ttcatcaaaa gaaatccgta cagcaacagt agcagagctc aacgctaaac 48240 gccgcctaac aaaagctgag caagcattag cggaggtgtc atgaatagtc aatttaagta 48300 taaacctgag tacaaacaga ctcaggaaat tcagtccttc tttgatccag cgttagtgat 48360 tctcaatgag ctacatgatc gtaaccgtaa aaatctaaga gccaaaggtt atgacgaaaa 48420 taatgctgca ataacgcgtg aagaattttc acaaactatg gcacagcgtt ttcgcattaa 48480 tcagtggtta gcagggcaga tcgttaatag tttggctaat gctgacttgg ttcaaaaatt 48540 tggtgggtat gtaaagccta aggtcggtgt acatgagtaa ttttgtgcct aattcctttc 48600 aagtgcctaa tgcatttgtt gacgaggttt taaataaaat ctctgatgct gcatgcaaaa 48660 tttatttagt tatttgccgt aaaactcgtg gctggaataa ggagatggat tccatctctt 48720 taactcaatt tgaagagatt acagggaaga gtagaccgac agttgttaaa tgccttaatg 48780 aattaattaa agttggttta gtcgtggaac aaccaagcac aattcatgga aatacattca 48840 aattaggtaa cgatactagc gttggtttaa tgcttaaatt ccctagtaaa aattttttac 48900 tacctgaaat ttatggccaa actagtaaaa attctttacc actgctagtt aaaaatttta 48960 actacactag taaaaatttt ttaccgctac tagtaaaaat ttttaacaca caaagtatca 49020 ctatcaaaaa caactctcaa agtaataaaa aaataaataa aaaaagagag cctgtttctg 49080 aaaaacctaa atcagaaaaa ccaactgaat ttaatccacg tttagttgaa ctaccagcat 49140 gtgtagatcc agagctgtgg aacaattttg ttgatatgcg tatcagcatc aaaaaaccac 49200 tttctgaaaa tgcagtaaag ctaatcctta aaaaacttat ctcgtttggc cctttggcta 49260 accaatcact ggaaaactca attatcggaa attatcaggg tgtatttgag cctcgccaaa 49320 atcaaattca ggaaaaccca caatctcata acgttcctga agaaccgggt tatttcactc 49380 aaatgtacgc tgagagcaac cgttcaaacg tgattgacgt tacgccagtg tcacaagatt 49440 ttggaggcta ttaatcatga atgaattagc accatttgaa agttttttaa aagaactaat 49500 tgcggcttac agaactaaat acgctgttca gttcaataag aattttccag tagaggggaa 49560 aaatgccgtt ccaatgcaaa tcgttgaaca gcagcttgct aaagcattgg ttggggttac 49620 acctaaccaa cttcaaagag gcttagcgct attttacgca agtacaaata catacatgcc 49680 taacttcgct gaattccgtg ctatgtgcat gggtgatgat tggtggagcg ctgagaaggc 49740 ttgggttaag gcttgtgaat acactcagat ctctcaacac aaaaaagtga cattgccaga 49800 cggaagagag cagaaccaag aaattacaac cttgaccaaa tttgttttag accaagttta 49860 ttcactaatc caagatggtg aaatgtacaa agccaaaatg gaatttatca aaatctatga 49920 tgaatacaag gctgaagcac aactgaaggg taaaacccaa gcttggtacc aagaaccaat 49980 tttattagct cagaaaaatg agcaaaaggt gcataagcct gtttcaaatg acgaagcgca 50040 aaagcatctc caatcattga tggaacgttt aaaaatcaat ggtcgtaaac ctgtaccagt 50100 acaaaagctt aaggctaagg aaaaagagcc agaactcaaa caagagctag gtccagatcc 50160 ttttgacaat ccgcacgaat acgcagagat gtgccgccgt gaaggtatgc caattcctag 50220 aaatattctt aagttaattg atggggcgaa tatatgaata aattcgagat tttagcgtgg 50280 ggtttactca tttcattttt tacagcagct attagcggtg cggtggtttg gtggtggttg 50340 gcgcgtaaag agcttgatga gaaaggagcc agccatgaaa gcaactaaat tgattagaga 50400 taaaggactg caatacgcga aggaaatcgt agattcagca cccgataacg caactgaatg 50460 gaacgagggt tatgagttcc aatgtggtca aagtgtagaa atcagcccag cagatcgtga 50520 gaagtatttt gtagatttgg ttgagcttaa acgtctggtg gagtctttga aaatcatcaa 50580 cgatttaggt ggagttgaga agctaacgcc tgcattcatt acgacagata agcatgttgg 50640 ttacacgcat gttcgcatgg tgggaaatgg gagattgagc tttcttgatg atttttgcga 50700 cttcattcca gatggttcca tttcaattaa gcgtgtgatg actgctatcc gcgaccacga 50760 atcaatatac ggaggcggtg aatctcatgc caactagata taacacaggc gagtatagct 50820 acgatcttga atatcactat ggagatatgt cagcaagcat ggagatgctt agagcacgtt 50880 taattgaatt gttgactcct catctgtctg gccgttatgt gaaatggaga gaagcatatt 50940 tcacatggtt tacaaagtgc ggcggggatt cggggtggat gttttgtgta ggtccacacg 51000 aatttcatat tgatggggcg ttaaggcgct attactcagg ttctattgat attacctaca 51060 accagaaaga tcgatgtttc ttggtgggtg agaaaaagaa agtcaaatgt aaggcttgta 51120 aggggtttgg cttcattcga gatgatgggt gggggcatat agataaatgt gaaatgtgtg 51180 atgcagaaaa aggagccagc catgagtgag tttgagggta aatctggaaa gtgggcttgg 51240 gagattcaaa aagaacaaca agcgaaagtg gaggagctgc aaaagcgttt agatggggca 51300 ttaaaagaga ctcaatatgc tttgcagtat gttgaagaag acatgcgcgg caatcatgaa 51360 tttctacaaa tggcaatgat tcgaaccctt aaagctatag agcaagtgct caaaggtggt 51420 gcttgatgtc atcagtcagc attgctgaat accgcaagtt atttcccata aagaaaaata 51480 aaaagcgccg ttcagcaaag caagttgcca gacaaccaag tgtgggtgaa gtggttctgg 51540 caacgcattt aagagcatgc aagattggat ttgaacagga atataagttc catcctgaac 51600 gcaaatggag agcagatttt ttaataaagg gttcaaagat tttgattgag gtagaaggcg 51660 ggatctggag cggaggccgt cacacaagag gcaagggcta tttaggggat atggagaaat 51720 acaactccgc agcaatgatg ggttttacag ttttacggtt cagtacagag caagttaagt 51780 ccggtatggc attaaagcaa attgaattat taattaaggg taaataggaa ggcgattatg 51840 ttggttgaaa agtttgattt tattgagtta cttcgccttg ctattgctca aagcgaaggt 51900 aaagggaaaa ttactaagca tgttgttttg ggagaaattg ccttattgcc tgcaggtgca 51960 aaaaaatggg cagaattact gcttgaacgt attgattttg agcgcattgc agaaatcaca 52020 gaaacaaaga aaatttatga gaccaggata attaatggta aggaatcaaa aaagcgtatt 52080 ggtgaaatac cgggtaaagt tgaaataaaa aaaggggaga ttaactcagc tgattttttc 52140 cgcgttagaa acgtactagc gggtaagatc catcgtgaaa tgatcaaaaa gaactttaag 52200 ccaaataatt gtcagggcga tttatcaaat gtggccaaag gtattgctga ggttgttttg 52260 cgtgggcgat tatttacaaa ggcaatgtgt ggccattgcc agggattagg caaattggag 52320 ttatttaatg aaaagggata tccaagcgga tctaagtttt gtgataaatg cagtggtacg 52380 gggaaacgcc catatacatt gcatgaaaaa attacgatcg caaaattaaa agtgtctaag 52440 tctggttatt ctgagcgata tgaaccatat gaattaattg ctgaagcatg tatcgaaaat 52500 tgggaaaaca ccattagaac tagcctggct agatcgtttc attttgaacc tgaagaaata 52560 tcattagctt gacttaaaca gaacggttga gtataagtat ttctaaaatg ggcgctttat 52620 acatggatcg cctgaaaaac ttaataaaag ctcactaatt ttagtgggct ttttgcgtat 52680 ctagagcatt gaaaatggaa aacacctggc atgctgacca agaaaaacca gaattacggc 52740 cagatgaaaa acctttgaat tgcccatttt gtggatctga ttcaatttgt acggattctt 52800 cacattatgg aaaaccagat gaagacggct ctatagcgtg ggatgctttc acatggtgtc 52860 atgattgtgg atcaaaaggc cctagtgctt gggcgatgat cgcttgggat gaaaattttc 52920 attacgacac tgtttatgaa gaaagatcaa ttgttaatta tgctattcgc cagtggaata 52980 cacgcaaata agatttttaa tctcgtgagg ggtgttttat aagcacacct ctcttttagc 53040 cggacggatt acggcgcaaa cggccccgct acatactagt tattggcggg gctttttctt 53100 tttggagtat gtatgactga atttcaaaaa attacgcatg agattagaca gctccaagta 53160 gagttaaatc atttaggaag ctgcaataca aaagacttaa cagcagaaca gatcgctcac 53220 atagatgagc gatttttttt ggccattgaa aagcaaagta aattaattgc tcgtctcaac 53280 aataagccag agggcttctt ataaatattg gtggtgcgat ggatcctaaa aagtactata 53340 tgctcaccag gaaaaaagaa agaaaaccca agccaaagag cacaccatta ccaaaagcca 53400 agcaaaacta tttagaggcc gaagcaactc taaaagaaga attaatcgag ttggccattg 53460 gttttgaaag taagtttcag ccgatccata ccaaacactg gcgctttgat tttcatattg 53520 tgaaattgcg tttgctcatt gaaattgagg gtgggccctg gtctggtgga cgtggtggaa 53580 agctggcaaa taaagcatgg agtcttgatc gatatgatca agctgaagag atgggttata 53640 aaatagagcg ctttcatcca gattctattt tgtcgggata tgtcatcaac tggataaaaa 53700 gtgaattagc gagaattgaa gatggagcaa ataagaccat ttccaccgac tgattttatt 53760 gatcaagcag atgaagaaga agcaattaga ctaacaccag caccagatct aaaaaaatgg 53820 gttgttgcta attacttaac tattggtgga cctctttata accccgatca tgatcacata 53880 ggtgaactgc ttcacgataa tgaagaattt ttagcattcg cgtgggcctc ttctgcatat 53940 aaaagcaagc aagctatggt gttgggccag tgcgaaaaag tcatgttcaa tgtcggtggc 54000 tggcgtaaag ctcgacaaga gcaacagatg cgagactggt tcggctttgt gccaacttac 54060 ttaataactg tcgacgcttc tttttgtgag cgtgcaaatg atacagagtt ctgttacttg 54120 cttgaacatg agctttatca cattggagtg atgagagacg aggacggaga aattgtttat 54180 agcgatagtt ctggtcttcc taagcactat cttgcaggtc atgacgttga agagtttatt 54240 ggcgtagtta aacgttatgg cccaagcaaa aatgttaagc gacttattga agtcgcaaag 54300 aatccgccgt ttgtttcgaa tcttgatatt tcaaaatgct gcggaaactg tgtaatcaat 54360 tgagcctaat ggctcttttt tttgcccatt ttgttatacg tagttatacg atgaggaagt 54420 tatggcgaca ctaaaagagc ctgtgaaaat ctttatagtt cagtctcttg cttgtcgtga 54480 tacacctcaa gaagtggctg aactcgtaaa acaagagttt ggcgttgata tagatcgtgt 54540 tcaagttgca acttatgacc ctacaaaggt tgctggtaag aacttaagca aaaagtatgt 54600 cgaactattt gaaaaaacca gagatgagtt tgataaaggc ttaattgata ttccaattgc 54660 taataagtac taccgattga agcaatacca aagacaactt gagaagacta gaaacgtcaa 54720 aacagcctta aaaattcttg agcaagccgc taaagacatt ggtggtcaat ttactaatcg 54780 ccaagaaatt acaggcaaag acggcggacc agtccaaaca gttaattcag aaattccagt 54840 tccaatggaa gattacttaa aagcgcggag ggaagtctta gatgagtact gatgcggctc 54900 gggataaagc catccggatc gaggcgcaag aagatttata tttcttcaca aggtacatgt 54960 ttaaggagcg ccgtggttat aaatggatgc aaaattggca ccacttagaa atctgcgaag 55020 ctttaatgaa agtttatcgc ggagagataa agcggttaat tattaacgtt ccaccacgat 55080 attctaaaac tgaaattgct gtaattaatt tcatggcttg gtgttttggt aagaatccag 55140 actgtgagtt tattcatatc agttactcgg caatgcttgc cgcaaataat gccttccaaa 55200 tacgaactct tgtacaagaa gaggcgtata gaaaagtctt tcctgagctt acattgcgtg 55260 atgatagtaa ggctaaagac ttctggagaa cttctcaagg cggtgtctgc tatgcgacag 55320 gtacaggcgg tacgattact ggttttggtg caggaaaact tcgtaaaggc tttggtggct 55380 gcattattat tgatgaccca cataaagcac atgaagcttc atcaaaaact attcgagaag 55440 gggtaattga ttggtttcag aacacactcg aatcgcgtac taactcgcca gatacgccga 55500 tcattgtgat tatgcagcga cttcatgaag atgatttagc tggatggttg ctaggtgata 55560 gaaaagacgg cgttcctgta gctggtggta acggtgaagt gtgggagcat ctatgtcttt 55620 cagctattca ggaagacgga tcggcactat ggccagcaaa acacaatatt caaaaattga 55680 gactaatgga gcaagcggca ccgtatgtat ttgccgggca gtaccgacaa atgccatcac 55740 cgccagcagg cggttttttt aagcctgaca atattcaaat tgttgaggct ttgcctgcag 55800 atgtattgaa acaagttagg gcttgggact ttggggcaac cgaaaatgaa ggcgacttta 55860 cagtaggtgt gcgagaagct ctaggcgcag atggttttac ttacattgtc gatgttacaa 55920 gaggacagct tggtccagac aatgtgaata agcgtttaga acaaacagca aagctagatg 55980 ggaaaaaagt ttctgtgcgt ttaccacaag accccggtca agcaggcaaa tcgcaagcta 56040 gttcatttgt gaagcttctt gcgggttata gcgtgatagc taagccaatt tcaggtgaca 56100 agcttacacg tgcacaacca tttgcggccc aagttaacgt aggaaatgta cgaatgctca 56160 aaggtgaatg gaataaggat tttattgatg agcttcgtca ttttcctaat ggcacacatg 56220 acgaccaagt ggatgcagct tcagatgcgt ttaatgaatt acatgaaggt tttgaagcct 56280 tctttgctga tatgggattt gctcgatgag tgatgtaact tttcaacatg ctgaatatgt 56340 taagaacttg ccatactggc aaaaacttga tgatgtttgt gaaggtgaag atgcagttaa 56400 ggctaaaggt gaaaaatatt tgccgatgcc aaatgcacat gataaatcac ctgcaaataa 56460 aagcgcttat gaggcttatc ttacccgtgc agtcttttat gaagtaacag ggactacatt 56520 aaatagttta gttggtgcag cttttgcaac cgatccaagt tttaaatttc ctccggaact 56580 tgctcattta gaacgtaatg caaatggtgc tggtttaagt acttatcaat tggctcaaaa 56640 tggaattcgc catttattga agcattatcg ttgtgcttta tatgtagatt atcctgatgt 56700 gccgccagct cgtaatctag cggaatttaa agcacaaaaa gcctatccga tgattcattt 56760 actaaatgcc cttgatgtag tgaattggga ttcagtaatg atcgataacc agaaaaagct 56820 ttgcttagtg gttatacgtg aatttaagtc tgagcgcggt gctgatggat ttagtaaaac 56880 cgaacaagag caatatcgtg tacttcgttt agagcaagag ggaaatgggg aatatattta 56940 ttccgttcag gtgtacacaa agggtgaaaa gggtaactgg gttggcggag agaagaagtt 57000 tccaacagat tacaacggga atttctggac ctatatacct tttacatttg taggtgcaat 57060 tgataattca gaagagatta aaaagccacc attacttcct ttggctaatc tcaatttagc 57120 ccattacaga gacagtgcgg actttcaaga gtccgttttt tatatggggc aacctcaata 57180 ttatgcgaag ggtgttaatt gggagtggta tgaccaagcc aagaaacgtg gcatctacat 57240 tggagcgaaa gtacttttgc ctttacctga aaatggtggt ttaggaattg tacaagccga 57300 ccctaatact cttgcccggg aagcgatgaa agataagtgg gaaaaaatga aggagatggg 57360 ggcgcgttta attgagaagg gctcgggaag taaaaagacc gctaccgaag cgaatagtga 57420 tgacgccgtt cagcattcag ttctttcgct ctgtgtcgtt aatatgaatg aagccttgtc 57480 agcagcatta cgatgggctg ctaagtttgt aacgcctaat gtggatgttc taactaaaga 57540 tgatttgatg ttcgaaatca gtcaagaatt taacaaacag ggttatttag ctgagttagc 57600 tcgacagtta tttgaagcag ctctacaagg ccgatcttca tttaaatcat ggtgggaata 57660 caaccaaaca ggtatgttcc ctaaacaaaa atatgaagaa gagcttcaga atgttgaagc 57720 agagcaagat gggactttaa atcaaaaggt agagtgagat ggcaacagat atcaaaaaac 57780 tatttgaagt actcactcag caccaggcct atctttatcg tgcttcatca aaaacggtaa 57840 atgagttatt ggctttattc aatgatgata cgagcaagat gctatctaag cttcgggatt 57900 tattggatga gcttaatgag tcggagaaag ttgctttagc tggtggtaaa tatacaactt 57960 caaatttaag ggaaattagg gatttgattg cccaatggtt tgccagtgtt aatttagcat 58020 tacctgaagc ttttgccgtt tctgctacgg cgctggctgt ttatgaggcc aattacgtag 58080 ctaagctcta tggagcaaaa attaataagc ctgatgggga aaaactattc ttatccgcta 58140 aaaaagttcc gttggcaggt ggcgctcttg tcgatgatct gctttcaaga attgctgaaa 58200 gtgcccgtca aaaggttgag tatgcaattc gagatggtat taattcaggc aaaactaacc 58260 aagaaattgt tcagcgtatt cgtggtacca aacggcttaa ctatgaagat gggatcttaa 58320 atggtaccaa aactgatatt gagcgaacgg taagaactgt gcgaagtcat gtagctaatc 58380 aagcctatct aaatagcttc aaccaaattg gctttgaata tgtccgattt gttagcgttt 58440 tagatggacg aacttctaag ctttgcgctt cattagatgg ttcagtgtgg gaaataaatg 58500 atccggcaaa gcgagtgccg ccgttacatc ctaactgtcg cagtatcttg gttccggtcg 58560 agaaggacgg tcaacttgtt ggcgaacggc catttgtaat ggacgaacgt agagttaaag 58620 acatccccaa agaagagcga agccagttaa taggacagtt agatgcaaac accacattca 58680 aagagttctt taagaaaaca gatgatttct ttcaaaggga gtggctaggg ccaaagcgct 58740 ttaagctcta taaagatggg aaatttgatt ttgataagtt ctttgatcct gaaggccgtt 58800 tctatagctt agatgatttg agaaagttgg atgaaaaagc ttttaaaaag ttgggtctgt 58860 aatttttctt atgttatatt ttttaaaaca tcagaattta tacaatatga aaacaatagc 58920 ttttgtatgt ctaaccctaa tttccatcac ttgtttagct gaaccaagtc aaaaatatct 58980 taaagaatat gatcgattgt ctgaagcttt ggagtcagca atggcaaatg catattcttt 59040 tgatcctgca actggtcaag taaaacaggc tactcaaggt ttagaagcta aaaataattt 59100 atgtagagct gcccaggcga aactaaacct caccacgttt ttaaaagaca atttagagga 59160 atctaaagag ctttataaat ctattgatgg tgcagagact ctagataaaa attatcttag 59220 tggacaacag caggaacaac aaaatctcgt ttcaaatttg aaaaaagacc ttgttggaac 59280 tggatttaac tgtgagtaat tattgccgat tacaggtaat tctaaactca cttaagacac 59340 aattttcacc tatataagcg cccaaatggc gcttttgtca tttatggagt ttggcttatg 59400 agtgaatcaa aagttagaca tttggtactt aaaagagttt cagataaatc ttctcatctt 59460 gctctttgtg acgaggaaac aggtattcca ttagctggat taaccgctgt aaaaatgaat 59520 tgtagtgttt ttgagggtcc agcgactatc acggcaacat ttgatgtagg tggtcctcaa 59580 ggcatccgct tagttggtga tgaacctaga caaaaggttt ggagtgcaaa ggaaacgtag 59640 cgaaaggcac tacaaatgcc tgaaaagcaa atcaatatgt cagatgctca atatattctg 59700 agcacaaaat gaattctggt gccatttctt caaattaagg tttcaagcca tggcaattta 59760 tggttttact tttgaaagat taaaagcaat tgcactcatc aaatagaact taatttttaa 59820 ccatagcacc ttcgggtgct ttttttgcga gaagaaaatg ccaagcccta ttatccaata 59880 tttccaatat gaacatttac ctgaacattt gcagcaagtt agtaagccaa ttggtgattt 59940 agctcggcaa atggatgagc aacttcctga cgggcctgaa aaatccacag gattaagaaa 60000 gctacttgaa gcaaaagatg catttgtacg ccaagcttta agtaaataat catttataga 60060 aatgaagcgt cctaaagggc gcttttttat tgcctgccga aagcggatgc taacggcgaa 60120 tccgggcgga tgcccatttt gtatatatag gttggatgac caatgaaact taaaacagta 60180 acaatcgacg gtaaagttta tgcggaagta gacggtgata agccgatcta tattcatgat 60240 gacggcaaag aaatgccaca tgatgcacca cactcggtag caacaattgc acgcttaaac 60300 aatgaagcta aaacacatcg tgaagccaaa gaagcagccg aaaaagcatt aaaagctttt 60360 gaaggaattg aagacccagc ggcagctaaa aaggcattac aaacaatcca aaatctcgat 60420 gataaaaagc tggtggatgc cggtgaagtt gagaaagtta aagctgaagc tatcaaagca 60480 gttgaggaaa aatatgcccc gattgttgcg caacgtgatg ctctagaagc ctctttacat 60540 aaagaactta tcggcggtgg ttttgctcgt tctaagtaca ttcaagacaa cattgcagta 60600 cctgtggaca tggttcaggc aacctttggt catcacttca aaatcgaaga aggcaaggtg 60660 gttgcatatg atccgaacgg cgaaaagatt tattcacgtg tccgcccggg tgaacttgca 60720 aatgttgatg aagctttaga gtcattggtt ggtggatacc agcataaaga cttaattctt 60780 aaaggtggta aaggaactgg tggcggtttt caaggtgggg gcaaaggtgg agcacctact 60840 ggaatgaaac gcagtgaaat gtctgtttct cagaaagcag attacatcaa agaacatggc 60900 aatgatgcct tcctaaaact accgaactaa tcattaaata tttggagata agtagttatg 60960 actacgacag ttaattcaga catgatcatc tataatcaat tggctcaaac tgcttattta 61020 gagcgtttgc aagataattt gaatgtattt aaccaagcct ctaatggtgc aattgtttat 61080 cgcaatgaga tcattgaagg tgatttcaat aaagaagcat tctacaaagt gggcggtagc 61140 atcaaacatc gtgatgtgaa ttcaaccgcc aaagtagttc cagagaaaat tggttctggt 61200 gagtctgtag gcgtaaaagt cccatataaa tatggtcctt atgcatcaac tgaagaggca 61260 tttaagcgcc gtgctcgtac accagaagaa tttgctatgg ttgttggtta cgatcttgca 61320 gatgcattgg ttgcaggccg attagagtac agtttagctt ctttaaaagc tgctatttct 61380 agcaatccag acatggttgc aaaaggtagt atcgttgttg atggccgcaa agcattgact 61440 cgtggtatgc gaaagtttgg tgataagttt ggccgcattg gcttatgggt gatgaactca 61500 gatacatatt tcgatattgt cgatgatgca atcactaagc aaatttatgg tgaatctgaa 61560 atcgttatct acggtggttt accgggaacc ttaggaaagc cggtcttggt gacggacgct 61620 gtaggtgata acgatgcttt tggtttgcag tatggcgctg tcactgtaac tgaatcacaa 61680 gtaccgggct tccgagctta tgacatcaat gatgaagaaa acttagcaat cggtatgcgt 61740 gctgaaggtg catttaactt agatattctt ggttatagtt gggatacatc gaaaggtgaa 61800 aatcctgacc ttacattact tggttcaagc gctaactgga tcaaatatgc aaccagcaac 61860 aaaatgacag caggtacctt acttgattta tcgggtacag cgacaactgg ttaaaaccta 61920 aaaattaaaa cctaaggggg ctaataagcc ctctttttta ttattaagag aaaagcgcca 61980 tgaagattat ctatacacgc attgcagcag cggctgcatt agagacaggc attattgcta 62040 accctgacta ttatgaaaac ccaaatttga aagcaaaaga ggtaattatt tacggtaatt 62100 atccaaagat tcaaaaggat tatgaatctt tggaagttcc agttgaagtt cgtaagttgg 62160 aagtgccaca aaaaacgact ttggccacag taaatgtcgc agtgggaatt acccctgaac 62220 ttcaagctgt gatggatgat gcaaaagctg aatgtgaaaa ggtagttgaa gaaaacactc 62280 agcttaagca gaaaattgcc atcttagagc aggccggtgg taaccagtca gagttgttat 62340 ctgaaaattc acgattaaaa gatgcagcag tcttagcaga taaagctctc aaagatgctg 62400 aagctcaagt ggtcggtata aaaactgaat ttgaagcttt taaaaatgat attcctgcaa 62460 tgcaggcacg tattgctgaa ttggaagctg gaaaagcggc agaaaaccca gctacagaaa 62520 cggcagctaa tgattttgaa aactggtcaa atgatcaatt aaaagagtat ttggctagta 62580 aaaacattgg ttacaagccg tctgcaacaa aagcagaact tcttaaatta atcccgaagg 62640 aataatgcaa tgagctttat tactgtagat gacgcaaatt caattttggg cagcgatttt 62700 gcaccagaca gtgataaggc tcgtctggtg aagctggcaa atgtgtggat gaaaaacaga 62760 attggttttg taccagatcc aattgatcca cttcttaagg acgcggcttg tgaaattatc 62820 aaaggaattc tggccaaagt aatttataac ggcaaagagc agcagttgaa gcgtaagaaa 62880 gttaaggctg attctgttga gtcagaaaaa gaatttcaag acggatctga agcaatctct 62940 agctttgaac agatagcaat tgattttatt gattcacttg atttgaaaga tccaaatgca 63000 agttttaatg gctttggcat accactttac agggcatgat atgggcttac gtgacgaaat 63060 tcaggcagac attgctgaag catttaatga tgatttagca gatgccattc atacctttac 63120 atgtgagcgg atctctaaaa cgaattggga tcctaaaact gaaacttatg ttgaagttaa 63180 agaaaactat tctggccgtg gcgttctgtt tggctcatac agtcaatatg agatccaaac 63240 acttggagta ctggccacgg ataaaaaggc aaccgtgctt caaaatgaag taactatgac 63300 tccaaaaatt gaagatgaat ggttaactgc cttaggctca ttccgggtaa ttaatattca 63360 acaggatcca gctaatacta tttggaaatg tcagcttcga aaagtgtagg ggctaaaatg 63420 gttaatcctg attatgttcc tgaatggtat atctcgcctt ttcaacatgt gcagtacacg 63480 cttgctcgaa atcaactaca catggatttg ttatttgaag atatggataa ggccgatcaa 63540 tttttggata tgggagcgga tgcgcaagtt agtacttttt ctgatggtgc atatgcaatc 63600 gtccaaattg gtgatacggc ggataaagac cgaattcaag tttatggatt gcttttacat 63660 gaagctgttc atgtctggca aaagattaaa aagctcatgg gtgaacgaga accgagctct 63720 gagtttgaag cttattcaat tcaggcgatc gctcaagacc tttttaaaat gtatgaagaa 63780 agcgaggtga atgatgggat ggaaggggaa aaagccaact gaatttagtt ttgatgtgtc 63840 taaagcagca gaagaccagg tgaagcatat tgttatggat actgtgcaat ccttagttaa 63900 tttaagtcct gttgatactg gagcataccg tgcttcacat attgtttcgg ttggatctgc 63960 tgatttcggc gtgcgtgaac ctgaaacaaa ccctattaac gacgcagcga ttcaggcaat 64020 gaagattaag ttaggtaatt tggtttatat ccagaacaat aaagcttatg gaccgcgctt 64080 agaaaacggc tggtctgatc aagcaccaca aggtatttat ggcctcacgt ttaactttat 64140 ttctcaaaag tacggtggct aaaatggcaa tgactttaga gcagacaagg caagctatta 64200 ttgatcgcat gcaaagcttt acgggtattg ctcaagaacg gattcagtat ccaaatgcac 64260 ctgactttac tgttccaaca aaaggtgtat ggtgccgttt aacgattgca ggtggcccga 64320 gttttacctc aggcattgca gataagccat gtacacgccg taccggtaat atcatgatcc 64380 aatgctttga ccgattacat actggagaga aggccgtaac ggttctaagc gatgcattgc 64440 tggctcattt tgaatatttc acaatcgaac acttagaatg tttgaatggc caatctattt 64500 atgcgggtaa agatgctgat ttcattcagt ataatgtgag cattgggtac aaggtgaatt 64560 gatatgtcat gtatgctgac tttagaagaa atcgaaatta aacggcaaga actggaacgg 64620 catcttgaag atgttatgtc tgttgagttg agcaaatggc aatctgaaaa caagctatgt 64680 gtttctgatg tgaatatacg cttggctaat gttgttagtc tcggagggcc taaacataac 64740 gttgttactg gagtaagtgt cgatttagat aatgagcttt gagttcaaga aaaagctact 64800 gcaaggcgat tatttttaat gacctcagca tattatcatt tgtgattaca ttctgttaca 64860 gtaatggaaa tttataacaa atggtaaaac atgaaaaaat caactttagg ctggggtgcc 64920 gcaggattag tagctttagg gatttttggt tcaggcaatg ataactctcc aaaacaaact 64980 tcagactcag aaaatgcgca gagtgcagta gaggaagtta tcgaatcaaa atatatcaac 65040 actaattctt taaatattag agataaacca aacggtcaag tagtaggaaa gttaggacgt 65100 ggggaaaaag ttgatattta tgagatgaaa ggaaactggg cacgtatttc cttaaattcc 65160 tcatcacctc agtggttatc aacaaagcta ttatgtgaaa cggatggctg ttttaaacaa 65220 aagtctcgat caaccacgtc aaataattat caggccttaa aatctcatcc tcatcattct 65280 gaaagaaaac agaaaaaaac ctactacgat agtgattgtt catgtgctgt ggtggattat 65340 tgcgtgggtc ctagaggtgg gcactactgt attacgagtg gaggaaacaa gagatataaa 65400 cctagatatt aattaatttg aattatgaga cctccatttt gagaggtact ttatgtctta 65460 atcactacca cctcatcggt ggttttttta tgtctatagg aatcacttat gagcaatttt 65520 gtatttaagc gtggtgacac tttcaactta aatcttcagc tagttgatat ggatgaagcc 65580 ctgcaatatc caccagatga tgtgcgccgt gcaattgatc ttacaggtta taccttcact 65640 tcacaggtta aagctctggc tgatggtgct actgtggcca cattaacttg tactgcatta 65700 agccaaagca cacagaaagg gtggctgaac attaaatcta gtgcaagcac tgcaacttgg 65760 cctttagggc tgtgtcagat ggatattaaa gctgtagtta gtggcactac acagcacact 65820 gaaactttga ctttccaagt gattgacgga gtaacagcat aatggcaaat cttgttttta 65880 aatttagttg ggatcatcgg ccattcccat ataacgcctc acagggcaag cggcagttta 65940 tgttgccatt tgcgtcaggt attcccaatc tggcacccaa cttttctcaa gtagttggta 66000 ctgcagctat ctctcaaggt ggaacggggg caactacagc ggctggtgca cgagctaacc 66060 taggcgcagc tgcaagtggg gtaaatagtg atattagtga gcttaaggga cttacaaccc 66120 ctttatcaat ttctcaggga ggattaggag ctgataatgc acagacagct agaatgaatt 66180 tggggttagg aactgctgct gtactagcgt caacaacaag tcaatatgat cctacgccgg 66240 gacgagcact aagagtcggt gattggggga taggggctga aggttctcgt gtatctgata 66300 tggttgctcc tcttaataat ggtttttttc gaacagatga cactttaaca aatgatactg 66360 gtaatagtat tggtccttat ggtttctttt tacactgtac ccgacgctca atgggtttat 66420 atacaaatgg aagtcattca tttcagcttg ggaaagctgc ctcatattct gccctgaagt 66480 atcgatttaa taatagtggt acttggtcta attggtttaa tttattgact gcacaaaata 66540 ctacaactga tggaaatggt tttattaaag ccgcttcacc agtcgttaag cttttccaaa 66600 gtcatattga gctaaataac gatgctgcca agcaaccgat cacttttgac aaattaggca 66660 ctggtgacta tctgattaag ggctctttag gctttgcaca ggaaggttgg tatatcgaag 66720 tacctaagga tgccaacggt aatacggtag tagcagttga atattcaacc ttagaaaatg 66780 gtgatctttc tattaaaact tataaacgta agtttgatgt ggaaaaggca gccattgtag 66840 ctgatctcga aaatccactt gatattccag aaggccgctg gattgatatc cgtctgcatg 66900 aagaacctga accagagcct gaagagccgt tgagtgaaac accagtggat ttccagccga 66960 ctaacttatc tcaggcagtt gctgcagcca tgaatggcgt ggaaccgcca gaaatctcag 67020 aaacagacga aacactttaa taacccgctt aaacagcggg ttttttattg cctaaatttt 67080 ggagaaccat aaatgagttc aggcgcaaaa attcgattat atgcttgtga ggaagcagtt 67140 ttaggaacaa ctcctgcaaa tccagtctgg tacaccgttc gccgtgttac tgatagtttg 67200 actgaaaatg ttactactga agatagcagt gaagtagttg attcacgttt tcgccaaggc 67260 gctgttgtaa cggaagccga agtaactggt caactagagt ttgaattatc actaggtacc 67320 tttgacttat tcttaaatgt tctcgctttc aataactggg ctgcaaatgc tttaagtttt 67380 ggtggtggag tacgtaagtc tcttaccttg gtaaaagtct ttgaagatat tggtcaagtc 67440 tttatttatc gtggtattca agtgaataca ggtgaaatga cgatccagac cacaggcaaa 67500 atcactggta actttggttt agtaggtagc tcatttacgc gacagcaggt taatcctgtt 67560 acaaatccta ttccagcatc gactcgccct ctggtgagta tgccaaatgt tgaaaagcta 67620 cttattaatg gtcaatcaat tcagggtaaa gcttgtctgc agacacttac catcaacttt 67680 agtaataatc tggaagcgat ccgttgtatc ggttctggta agtacacgcc tgagttttac 67740 ttagagaaaa tgatggatat tggcgtaaat gctaatttca tgttttcagc aacatctgct 67800 tcttggattg atgctattaa aacccgtgat gtatttacat tgaccttcga tattacagat 67860 accaaaggca gtaagtactc gtttaacttc ccgcaacttg aagttaagga agcaaatcac 67920 cctgatggtg gtggcgatga cattattaca atagatatca attttgccca agtgcgtacc 67980 agtccaacga ttgtacgtgc tcttgtgtaa tcaacttatt cagtaacaaa gcctatggaa 68040 acccatgggc ttttttattt ctaaaaatta gaggttgtta tggctttaaa agtcggaatt 68100 attaaaagct cagacgtatc aaaatggtgt gaatacaagg gtgctgatgg cgatgtacag 68160 gctgagttca aagtccgtgg tatcgcttat aagccttttc aggtagctat tgaacgggca 68220 ggaaaccaga tctcgtctaa aggctatgat gtgatggtca aagatgaaga tgccaagctt 68280 taccacgagc ttttaatgga tgcatgcgcg gcccacttaa tcgaagactg gaaaggtgtg 68340 gtatttgccg aaatcgtaga cggtaaaact gttgagtccg aaaagccata tacacctgag 68400 aatgcctcaa agcttcttaa tcttggtgat attggtattt caatctggct attcattaaa 68460 gaacaggccc agaagattca ggaagacgca gacaaggaca aggctttaat tctgggaaag 68520 tcatggagct ctacaaatac caaaaaacgt atgcgtcgaa aacgccgcac gaaatcgagc 68580 aaatcaagtt cttaggcggc cgtattccgg atccgccaga atattcgtat gcggctgatt 68640 caattctttc ggcatttagc actatatgtc gatccagacg ttatgagcaa agcataccgt 68700 tatctttaga tcagcaggct atcaatgtct atgctgagca taatgatttg ccagtggctg 68760 ctcatatttt taatgactgt atttttgcgt tggataattt gtttttggag gagtgccata 68820 agaagatatc aaccaaaagc aaaggtaagt gaccaaatta ggtattgcca gggactgaaa 68880 agcctaattt ggtcaaaacg tcaaacaatt aagcagttgc tcttaaacgc gactcaaaat 68940 aacgcagtcg atgttacaaa atacttgatc tggattgaca gaaaattacc tttaaggtgt 69000 tgcgcgtgat tatcaaatga tgaataatca ccttaccgtc aatatttgac ggttcagcat 69060 tcttttactc tttccaagaa ccttggtgtt tgcttgtatg tgtttaacat taactgaagc 69120 taaacaaaaa cttagagcat ttgctagaga tactagcaaa atcaagttaa ctgcacatgc 69180 aaaagaaaga atgaaagaac gctgtatctc tatgaagcaa attatttgct gttttgagca 69240 tggagatatt actgaggggc cgtacccaaa tactcgtggt gattgccagt taaatgtttc 69300 tgttcgcact gcaggcgaat acataacaac agctgttgca atcaagcaga gcgagaacgg 69360 tgaattctca gtagtagtca ctacatttag agagtaggct aaattatgta tcactatgaa 69420 gaatgcggtc tgagcaatat ttggctgcgc aatggattta caattgaaaa tgatgaagac 69480 tatggtgaac tcgtatctat tgaatctgtt catgagcttc ataatgccat tgggttgttc 69540 ttaattacgc aaaagcctga cttgaatggt gaggaaattc gttttttacg taaagaacta 69600 aacttgtcac agaagaatct tgctgggctt ttaggagtca gtgagactag tattagacat 69660 tgggaagctg atcgcggttt aattggtaaa cctactgagc tattacttcg tgcattatat 69720 aaagagcatg ttcaaggtga tggcaaacta agaagtatga ttgagtcatt aaatcatcag 69780 gaacgaactt tagtaccaag tgaaattagt ttttcatatg gaaataacca ttcatggcat 69840 caaaccaatt gtgaaatagc ttagttagtt ttatttgata gaaaccacct tcgggtggtt 69900 ttcctttatg tgacatttag taaccagttt gttaaagtta gtacacttta taacaaacgg 69960 tgaaattcat gaaaaaaata ttggctgcgg gtttaattgg tcttgggttg gtggggtgcg 70020 ctactccagc ctataattat caagctatac ctaaaaatat aagcaaaccg ccaattggat 70080 cagttaataa agcatttgta ggggatcaaa tgcttgaaca gggaatggtg gttgatcgtg 70140 aagttctaaa cgtccctgaa aatattaaaa ttagttttgc ttattcactt acttcaggca 70200 tttacttaaa aacaggcaaa aatgaaaaag ggcaatattt tcagccattc aacactgtca 70260 gtggtggggg gatggttcag aaaaaccctt tagctgaccc atttaaagta gttatgttag 70320 atactgaagg taagctctgt gtagtaacag tatttaatgc aaaaaactgt actgataaac 70380 atcaagctac tatgaagaca gtagcaattg catcagataa ttccttccaa caaacattaa 70440 tttatagtgg aaaatttgga aataaaatta atgtcgggta ccgtgaattc tcaagtaatc 70500 aagcacgtcc tgcattcaat aatgatgttg aatatgattt aagccaatct aagcaaatag 70560 gttataaagg tgctttattg gaagtaattg atgccactaa tcaagatatt acttacaaag 70620 ttttgaagaa ctttaacaag gtagattaag atgagtgcac cacaatataa accaatgaga 70680 gaaagtgaag tttgtaatgc tatcgggtgg gtgttaatag ctcttggctt tatcgcaggt 70740 tttttattta ttcttgcatt tggtcgaatt gaagtagctt cttactatgg taaagaaacg 70800 gtttggtctg gagttatgat agcaacagga atcggaatta tatttaatgg attccttgca 70860 ggctacttat ttcaaaaagt agctagtatt cttcgttacc atgagaataa ataatatctt 70920 gcataagcac cctaggatgc tttttaaaat tggtttaact accctgcttg gtaattatat 70980 ttaacttaaa aagaactacc cactcattga gtgggttttt tattgcctag aggaaagtaa 71040 aatggcacaa gaatcccgtt tggtcattgt tattgattcg caaaatgctg aacgtaatgc 71100 gcgtaatcta ggcaatgaac ttgttagcat tgaacgtaaa ggtgaatttg catctaagtc 71160 tatggacagc ttgtctgtag ccaccagagc tttagctgga cacatggctg gtttattaac 71220 agtaggttca gccatttcaa agatggatac atatactgga ttacaaaatc gccttaagtt 71280 agtcactaac aatcaagttg aactaaataa agcaacggaa gacactttcc gaattgctca 71340 aaaaacctat tcagcttggg attctgtgtt acaggtttac cagcgtttta gtgataatgc 71400 caaaacttta aacctcacaa tggatgacac agcacgttta actgaaacag tttctaaagc 71460 tgtagcaatt agtggtgcaa gcgcagaagc tgctgatgca gctttagttc agttcgggca 71520 ggccttggct agtggaacgt tgcgtggaga agaacttaat tctgtaatgg agcaaacccc 71580 agcactagca aaggctattg ctaaaggtat gggtattact gtaggtgaat tacgttcagt 71640 agcagctgaa ggaaaaatta cttcacaaga aattgtaaaa gcgcttagaa atgtagaatc 71700 tgatgttgat gctctttttg ctaaaacaga tatcacaatc gggcagtctc tcacactcct 71760 aaacaacgag atcacaaaat ttgttggcga agcaggtaag ggaagtggtg cggcacaggt 71820 attagctgga tcagttcaaa ctcttgcaag taatttagat ttaattgctg atggggcttt 71880 agtagttggt attggatata tcactcgtgc aattttgatg aagagcgctg ctattaaaga 71940 gggaatggct tcaactttag cgagccgcca agcatctgta ttaaatgctc aagcagaata 72000 tgcagaagct accgctgctt tgaatgcagc aaaagctcat ctcgcgaatg tgcgagcaac 72060 aaatgcagaa acccaagcta aatttggcgc aacagcggca gcaactcgat acgcacaagc 72120 acaggcagca gtaactgctg ctacaaatgc acaaacagca gctcaaatta agctaaatac 72180 tgcaacttca attgcaggga gactagctaa aggggcgttt ggattaattg gtgggtgggc 72240 tggagttgca acattaggag taatgggatt agcggcagcc tattcttatt ttaataataa 72300 ggcagaggag gcaaagcaaa agcttgctga acaagctaaa gttgctgaga aagctgatga 72360 ggagttaaaa aaattaactg gcaatgataa ggctaaagca gttaatgatt taactactgc 72420 ttttaatgca caaaataaag cattagagaa atcatcgcgt gctgtagggt ctgcattaat 72480 tgatatcgag aactatgcac gaggaaatag ggaggttgaa aaaatttccc aagaagcgag 72540 aactggaact atcagctata cagaagccat tgaacgtcta aataaaatta agttgcctac 72600 agatctatat gaaaatctga aaaaacaggc tgcgcagtat gatgacaatg catctaaagc 72660 aagtttatca gctgagaaac ttaaattatt aagagttgaa gtgaaacttg gaggtaatga 72720 agcacaaaat gcggcaattc agcatcaaaa acaagcggat gctttaggaa atactgctac 72780 tgaagcagaa aaggcaacta aggctttgca agattatcaa gccaagcaaa aagatagcgt 72840 tattgattca atctataaat caggttggct tgataaaggt tacactgttg ctcaagctaa 72900 tgccatttta gaactgcaaa aagctaaagg aatgagtgca attttgtcta aagatgaaat 72960 tgatagcgca cttagaaatc tcaagatcat cgaagaacaa caggagcgag aagataaatt 73020 aactgaagct aaaagaaagc agacgcagga aattgaaaaa caagcaaaac ttactaaacg 73080 cttggtcggt atttccggtc aatccggtat tggtactggt ccacatcttg acgtccgata 73140 tggtggctca ttgtcaggtc agaaagtttc taatgaacat ctggctcgat tacaggcggg 73200 aggaaaacct ttaacttcct acaagatcag ttctaattat ggtccacgaa aagccccaac 73260 taaaggggct tcttcatttc ataaaggtat tgatttttca atgcctgaag gaacaccaat 73320 cacgaccaat gttgctgtga aagatatcaa gacatggtat gacagcaagg gaggtggtta 73380 tgtcagtgaa gtgatctttg aggatggagt gtctcttaag cttctacatc aatctcccaa 73440 gatgcagagc aaggtgaaag gtggtgcaag taaaggaagt gataaagcag ctggtgatat 73500 tcaatctcaa cttgaacgtc aacaggattt gcaacggtca cttgaaaatg aggtggctag 73560 tgaagtcgga cggattaaca ataatagaaa ggcaagactg gaggatgttg ataaagcaaa 73620 ctttagcccg gaacgtactg cagaaataaa ggcggaaata aatcgtcgtg cagataatga 73680 tattgctata gccaaacaag cccttagaac gaaattggaa gactataagg agttccagaa 73740 aaccgaggaa cagttactag aagagtcctt taaccgtaaa aagttcaatg cagctcatga 73800 ccttgaatta agtaagtttg agcagaagca agctgttgaa ttgctggaac agcaaaaaca 73860 gcaagagtta gggttattaa aactagctca ggaacagcgg ttgtttcaag cccgtttatc 73920 tctgctttct gaaacgcaag ccatgcagga acgttacaga ctcgaacggg aggaaattct 73980 taagaatacc aagctttcta tagaagagcg gcaaaagcta atcgcattat ctaaagccaa 74040 tcaggataaa gagacacgcg ataaagtgaa taatgctgtt caaaactggg gtggtatcca 74100 agcggatatg aatggtaccg gagaattttt cagacaggat caggaacgat ttagccgttt 74160 aaatgctgca aatgatttag cagatagtca atttgctgct actgatcttg atgaaaaaaa 74220 tggtttagat gttctaaatg cacacatgga agcaggactc atcaagcaac aggacttcga 74280 aaaccggaaa acagctatca ttcaagctgc tcaggaccaa cgcaatcaga tcgctgccga 74340 atatgctcag aatgctcagg atattgaaga taagtatcac caagatcgat tgaatgctca 74400 aattgctctt ggtggccaaa tgatgggttc actcacatcg atgtttggtt caatgtttgg 74460 cgagcaatca aaagcataca agatcatgtt tgctgctgat aaagcttatg ccattgcagc 74520 tgcaggtatt tctattcagc aaagtattgc aaaggcggct agtgttggtt ttccagcaaa 74580 tatcccatta attgcaagtg ctattgcaca aggtgcaagc atcattgcaa acatccgggc 74640 aattaaagat caaggctttg ctgacggtgg ttacactgga tcaggtggga aatatcagcc 74700 tgctggtatt gtccataaag gagaggtggt ctggtcccaa gaagatatta aacgctgggg 74760 cggtgttggc ttagtcgaga aaatgcgtaa gagtgcaaac cctgaagctt ttctcaataa 74820 caatgccttg gcagatagtg tcatgcgccg tgcaatgatg agctctagtg cctttataga 74880 aagccaaaag caggctgaca tctttaatca accggttcaa gatactcaga ttatctataa 74940 gggtaataga gacacaccta aattagcttc ttctggaaat ttagacttat tccatgatgg 75000 caaggtctac ttctcatcca atggtttagt tcaggatcgt tcaaatctgg atgatgttca 75060 ggactttact ttaggaagta cttcacgccc tcaagctgag atgatgcctt caattgagcc 75120 agcttcaccg acaatcaatt tcaaaattga agtgattaat caggtgagtg gggcgacagt 75180 tgaagccgaa caactggacg agcaaacagt ccggatcatt gttaaagatg aactggataa 75240 gcagcttcca agaacggtac ctaagcttgt aagtgatcaa atcgcaaatc caaactcaac 75300 cattagtcgg tctttgactg agaatacgac agcgagaaga aatcgtactt aataatttga 75360 acccttttcg gagggttcat tttcataata tttaaatttc aaggtgatag agtctgttgg 75420 cattaaaatt gatggttaag acatgaaaaa aataattgta atttctacaa cacttttagg 75480 ccttacggga tgtgccattc ctgcggtaaa taatctcgta agatccacaa atatgtatca 75540 ggatgatgtt tcgggaaata ctgcaaattt aagggtttat agaagtaata ttcccatggt 75600 gcagttctat attacttatc aaaataatga gggtgaaaaa atttcaaaaa acctaataac 75660 taagcagatt tcaaataatt taacaaagta tggctctatg catgagccca aaaaattaaa 75720 tatgcctaaa cccacaatca gtttaaataa tggtgaagag ttttttgagt ttaaagtacc 75780 cgcaaataag aagttaactt tcaggcttac ttctgttatt gggtcaacta ctatgtatag 75840 ttgtgatgta aaaatggact atcagttgga aagaaatgga aattatgaat tgatccgttt 75900 aaaacagatc aaagattttg tgaatccagc tttactgact gaaccatctc aagatgaagc 75960 ctactgcaag tttgtagtaa aagagatttt tgaagatggt aaagaaacta ttattaaatc 76020 gatttcttaa tgttaaatcg tttttgtaat taatttaaat atctaaacct tatttcatca 76080 aaccaccttt cggggtggtt ttttattacc tgaaggaaag ttatgtacaa gttaaagcta 76140 aatcctcaga ccagcggcta tggcgtaaca ccgggtgatg atgtgaaacg tcagcagatg 76200 gatggcggtc gtggtcgcta ttacatcgat gtgaaacgta acagccacat tgttgatgtg 76260 aactggaact taagtaaaac cgatttcaat aaaatgatgg ctttctggcg ggtctaccag 76320 aacaaaccag cctcatttta tgcggatttg gtgattgatc agggaacacg tcagcaatat 76380 ctatgcaatt tcattccaaa ctcgttcaag accaatgaag tcaacggcaa cttatatcgt 76440 gtgaacgcgc agctcgaagt tgttcaaaac cagcctaacc tgaatgccga tatagcatta 76500 attaaagatt gggaggtcta atggataacg aatatgccaa gttctttttc aatcggaaag 76560 ttgatgtcta tcaactggag tgtattgagc tatcacatcc ttcttttatg aatacttatc 76620 gggtagtccg taatgatgac cgaggtgtct atgtacaaca taaggaagga tccggtcagg 76680 tctattatga gttcttgcca gtctctatac aaagatccgg aatgcttggt gatctggacc 76740 agacattaac cgtttctatc tctggtctag gtgatgtgat gcctgatgag tttgaacggg 76800 taatcgaagg gcaatatcca gatgtaaagc caaccgtaaa ttaccggatt tacagttcag 76860 acaatctgaa ctctccaatg ttttatttac ttggactgca actctcaagt gtcgccatga 76920 accataaagc tgtgacattc aaggctgaat caccacgatt aaataccact aaaactgggg 76980 acatttttgc actggatcgc tttagtggtt tgaagggggc tatatgaaaa gtcatgatca 77040 tttgctcgat aggcaatatg acgaggatca ctacaactgt gttcactttg ttcatgaagc 77100 tgcaatggac ctatatggca tagatcgggc tgaagcgctt gaactcttta tgcagcctaa 77160 gggcaaaatt acttttttat cttcacggtt aaaactttta aatccgctac ccatgcccaa 77220 ggaaggctgc atagtcgcct tccatccgag acaaagaaat aagcccccgc atgtggggct 77280 ttttcgtggg caaagattct tcacctcatg gaaagcggag tcacttattt gcctgaagag 77340 gttgtgatgg aaatggggtt taatcgggtc agttattatg attaaagtta tttataaaaa 77400 agacgctttg tctgaagaaa agacaattga gcaggctcaa accattgggc aatggctcac 77460 ttcaaaatat gaacatatgc ctgagcatgt ccgtatcttt catactacaa gcaatatgga 77520 tcatgccgaa atttcatttg cgaatgaagt cacaccaaag aatgcatatg acttaaagca 77580 gcttgatttc ttaccgggca cttttatcgt agttgagaac cctaaatggg tcgcggctat 77640 tgtttcgatt gtgattagta ttgcgatcgc atttttaatg ccaacgccat caatagcaca 77700 aacgactcaa aatactaacc agtcttcttc agcaaacaat gaactttcta accgggaaaa 77760 caagatccgg gtgaatggtc gtattgctga taactatgga gctgggtgga atactcccga 77820 cctaatcgca gtaccttaca aggtatatga aaacaacgtt gaagttgagc atgtagtggg 77880 ctgtattggg cgtggacact ataaaatcaa tggagcttat gacggtgaaa ccaatattgt 77940 cgatattgct ggcgcatcgg tagaagtctt tcgaccaggt gtagatattg tttcaggtga 78000 gccatatttc tcgcttggta ccgaaattac cacgccgcca ctaacggttc agcatcaaac 78060 ttctgttaat ggccaagttt tacgtcctgc tgatacacaa tctttagaag gtacgaacta 78120 ccttcatttt gcatatccaa acgagattct tcgggcaacg gcaaacaaca cagatttaac 78180 cactaagttt gtaagtaatg accgcgtaga aatcaccaat gcctcattca cgtttaatgg 78240 ccagactttt gatttaaatg gtacttatag cgttctatcg gtagcagatg atcgaatgac 78300 gttatcaaat ccggcggccg ttaatgctaa ctggttaaag cttaaagagt taaataacca 78360 acaaactgca gctttgtcac caaagatcag ttcaataggt gaaaaatgga ttggtccatt 78420 cattctggac aatgttgaac gtagccgggt gctgtgtaat tttgtggcca caaatggact 78480 ttataccgtt tcttcaggtg ggaatcaggc cgctgttaac gtcacgattg aagttgaagt 78540 aacaccggta aatgaatctg gtgcagcgat tggtaatccg atgctgaagc agatcatttt 78600 gaaaggttcg gcaaagtcgc gtcaaaccgt tggcgcaacg ctggatatgg tgacatttca 78660 aggtcgctgt agtgtccgtg cacgtcgttt aacaccaaca ccggcggtta caacggtagt 78720 agatgaagta aagtggcagg cgctttatgg tgcttatcct ttgcaaagca cagtgtatga 78780 acatgaaacg gtttttcgtg cgcgcactta tgcaaccact ggagctttat ctgttaagtc 78840 ccgcaagatc aattttgatc ttcagcggat gttaccgact tttaaaaacg gcgcaatgac 78900 gacagagcta tttccaacat caagctttgc tgatgcattg gtttcaatgg cactggatga 78960 caagataggc cgccgtacga tcgacgaaat agatctggaa aatatctatc ggacttataa 79020 cgatgtagtt gattattttg gtacaccact tgcggctgag ttctgtacta cgattgatga 79080 tacaaacctg tcttttgaag agctggtcac caatctttgt gatgccgtgt tttgtactgc 79140 atatcgtcaa aataataagc tcaagcttta ttttgaacgt ccaactgata actcggtaat 79200 gctatttaac ttcaggaata ttattcctga tagttacaag catgatctta cctttggcgt 79260 gatggatgac tacgatggac tgatctatga atacacggat ccggccgacg atagtcgtat 79320 caatatctat ctaccggata aaggggccaa gaaccccaaa gaggtgaaat ctgtaggtgt 79380 gcgtaacaag tggcaagctc attttaatgc gtaccggctt tggaacaagc ttcgcttcca 79440 gcgcaaatcc attacctttg atgcggcacc tgagtcagaa ttactggttt tacgtgaccg 79500 gatcgctgta gctgattatc gcaatggtat tcatcaaagc ggtgaggtgg tacagcaaga 79560 aggtttaatt ctcaccctaa gccatgatgt cgatttcatt gcaggcaaga gttatgtgat 79620 ttatttgcaa atgggggatg gtaccgtgga cctgattccc gttacgccgg gttcagccaa 79680 gaacaaagta gttttagggc gtttaccgaa cggggcctta aagcttagtc ccgatgactt 79740 tgtgaatact atctacaccg tagttaatga cgataccaaa ggctcactgc cttatctggt 79800 tgcaaaaaga gaaccggctg accagttctc taataccatt actgcaatta attacgatga 79860 acgttattac ctcaatgaca aggactttat tgatgtgccg gttgatgatt caccgattta 79920 cattcgatat gaccagctgg atattaatct ggcacgttta tatcagatgc aaagagggga 79980 tttgccaacg actggagaaa tcagttttgt agttgaagca ggtgcactag tttcaagttc 80040 aagttcttat cgaccggaaa ccagatttgt ctataaattc gactataact ctagtcctgc 80100 aaaacgagag tatatcgttc cagctgcatc agaattacct gctattgata ctggtgagtt 80160 cccacctgat ctcgtggtaa atttgactat taaaggtgct gttgttggac gtggtggaga 80220 tggcgggttg ccacatttgg catttggtgc atggtctacc gatccggatt ataactttac 80280 taaaacccgc cgtgacggtt ttcagggagc acccggttta ttaaaccggc acagtaaact 80340 aaacctgatt attgatggtg gaactctggc tcgaggcggc tcaggtggtg gagcaacacc 80400 aagcggtatt tatacaggat tatcgtatgg agttcagggt attcccggtg gagctggagc 80460 accttttggt cgggttatga ccggacaacc tattactaac gattcacaag actggcgttg 80520 gtacttaaat ggtgacttta tggttgtcaa agtaaccgat gccgaagctt cggtacccgg 80580 taaaggttac cgaacccaaa atgatcgata tggatctcca ttgtctggtg atggtggagg 80640 ttggggccag cgcggtacca agtccaccaa tgatggaaca tggaactggc aataccatgg 80700 cacaactgaa ggccagccgg ggccgggtgg acctgcaatt gttggggtgg caccacttac 80760 aactcaattg atcaatggag ggaaaattct acaaacactt taaatcttaa aagaactttg 80820 agcacccaat tcgggtgctt ttttattgcc taaattttct ggagatataa atggaaccag 80880 tttcaacaag cggtttaaca gcaattttaa aattttatgg tgcagcaatt atggtgacgt 80940 tagcggttgc tttagttgca gcagttgtat tgatgacacg tatgccacga tcacctcaag 81000 aatgggctgt aggacttatt tgtacggttg tatcaagttt ggctggcggc tcattcatta 81060 ttgtgaagtg ggggcttcat gaatgggtta ctgatgtatg gggaatgatt gcacttggtg 81120 ggttcttctt tgtttgtggt ttacctggtt gggctttagt ccgttggatc tttaatttca 81180 tagataaaca ggaagggaaa acgattgttg aagtgattaa agagtttaag aaagccagaa 81240 aagacattga aaacagctaa tgccgccttc gggcggtctt gtttagaagt acacgtataa 81300 gagagaaatt acctgttgac actgcaagcc gctgactact acgaaaacct attgacgacc 81360 aatattatga aacgaccacc ttcgggtggt tttcctttat gtgacattta gtaaccagtt 81420 tgttaaagtt attatattta taacaattgg tgaaattcat gaaaaagata attttaggga 81480 gcatgttagt ggctgttttt tccacatcat tttcacatgc tttagctccc aaaaatggag 81540 atgagccaac ttattgtgag cagattgttt cggtccatgg tttattaact agagcacaat 81600 ttgaatgtgg atatagtgaa tataacaatg agttaatctc agattcagcc aagtgttttc 81660 agcatgaact tggcgaagaa tatggaaaaa aagtccttat atttggcatg aaagaatttg 81720 accgaaatgt aaagaaagac gggaagaata agatttgtaa tagtttatta aaagaatttc 81780 cagagtatgt aaggaagtaa ctgatgaaaa agctactacc aattgcattt ttactcacag 81840 catcatttgt aactcactca gccgatacta atgataaaca ctgtagagat gtgaataaac 81900 ttgctgaaaa tgtcatgctc tttaggcagg aaggggtttc tgtggttaga caaatggaga 81960 tgatagagag tatcaaacca agcagggatt tcaaaaggtt aatggagatg atggtcgagg 82020 aagcctataa agaaccaaag tttggatcag aagagtataa ggcggaagca ataactgaat 82080 ttgcaaacaa ttggtacatt cagtgcaagc aagcaaatcg aaataaatag agcactttaa 82140 ggtgctctaa ttattgaaat tgagaaaagt ttataagtag gtttttatga gaaagattat 82200 tttattgggt cttatttgcc ttcctgtatt cgcatatgca aatagttgcg aggtggcaaa 82260 aagtaaaatt aatataagcg gtttagcatt gggtaaatcc attttatcac tgaaagcaga 82320 acatcctaaa aatttgagca tagatcatga gactaataag gcaaatatta actatgttca 82380 ctctaatgaa tttgaggatg cttttagtgg aacaccagct accaatgcgg gattcatttc 82440 ttttgatggg aatacaaagt taattaatgc gtttagcgtt agttttggtc acttagataa 82500 ttttagtgct aataattata aaaatgggtt agtggcgttg tattcactac caaaaacagg 82560 gtggatagaa tcaaagagta atggggttaa agtttttaaa tatgaatgta cggattattc 82620 tttagagatt aattacaatc cagaaagaag tagttttatg atttttaagg aaatttagtt 82680 ttatgttctt aagcacccta gggtgctttt ttaatgtctg atttttctga aacagtaatg 82740 gtgtaacctt tcatacggct agctaactcc atcattagag tttcggtagg gatcagttct 82800 acagtttttt cataattttg attttcaaag cttttttcaa gacgggcaac aatgtcggca 82860 ttcattgatc gactgtttaa ctttgctgat tcaagtattt tttcttttag ttcttgcgtc 82920 atacgcattt tgtattcaac gtctgagctt ctagccatgg tcctatactc gaataaattt 82980 tatttataat aatatcccca atggggattg acaagaagtt tttaaagtct taaattgtaa 83040 aagtccccat tggggatgta aaaagccccc aactttctga cggcaagggg cttttatcaa 83100 caaccatagg aaaggatatt gatatgtcta gtttagcatt aagttttaat gaagtgaaat 83160 tcaatcccgt gccacggcaa gatggccaga tttggctttc ttcaggtgaa ttggcacaag 83220 cattaggata taaacaagag aacgcggtca gtaaaatttt taatcgtaat tctgatgaat 83280 ttacggaaaa tatgacacaa attattgata atcctcggct acccaatttg ggtatgcgga 83340 tcttctcact acgtggctgc cacctaatag caatatttgc tcgtactgct gtagcgaagc 83400 aattccgcaa gtgggtactt gatgttttag ataaagaagt tggcacacca gttgccaaaa 83460 cccacaaatc cgaacgtgaa cccctaacca atgctgtaaa tcttcttgta gctaaaacta 83520 agcatttgaa ttacagcgat gcttataaat tagttcatca gcgtttcaat gttcagcata 83580 ttgatgaaat cccatatgac atgattcctg ttgcagtgga atatgttcat catctgattg 83640 cgatgtacag tagtgcagag aagaaggctc aaggttcttt atttgataat gaaacattgg 83700 gtttggttaa ggatctggta gatgcaatta tttcccaaaa ctttgtgaca agcaaaatct 83760 atcgtgcaat acacatgctt agtaatgaac aaggtcacta cttagctgaa tatgcgttta 83820 aaaccaatat tgcagttcta aaactcactc gaacaatgga tttaagagga cctcttaata 83880 gagaaatcat tagtgatgat ttaaaaacca taagctacac aacaggtaat caacattatg 83940 gcgaccgttg gtttcaccca ctgatggagt caagtcgatt gatgggagta cttgaaattt 84000 caggtagtct gattcgtcac taataaaatc aacttaacaa aacccactca tcgagtgggt 84060 tttttaatac ccaaaacaaa accccagtag cgctaactac cggggttttt cattccaccc 84120 accgacgaaa gtaagaggaa agtaaatcta tatggagcat tttaaaccaa tagtggagct 84180 tataaaagtg tctattgaaa agtatggctt atggcaaaca atagttgcat ttattctttt 84240 gttttccgtg ccaatcttaa tgtggaagtt ggatgtaatt attgcttcta taaaagcatg 84300 aaccaacttg aaaaaactgc gccaccttcg ggtggctttt ttacgtctaa aggaaagtga 84360 aatgaacatc gaacaatatc ttgatgagtt gatcaaacga gaaggcgggt acgtaaataa 84420 cccagcagac cgtggtggtg caactaagta tggaattact gaagcagttg ctcgagcaaa 84480 tggattcaaa ggtaatatgc gagatttacc tctggatgtg gccaaagcaa tttaccgcaa 84540 aaactattgg acagctccgc gatttgacca agtaaataca atcagctcag cagtggccga 84600 agagcttcta gacactggtg tgaattgcgg taccggcttt gcaaaacctc ttttacaacg 84660 agctttgaat ctcctaaata acaatggtaa agcagggtgg ccagatttat cagtagatgg 84720 gatatatggt ccggcaactc ttaatgcact caaaacttat ttggtcaaac gcgggaaaga 84780 aggagaaaaa gttttagttc gagttctgaa tattatgcaa ggtcagcgtt acattgaaat 84840 ctgtgagcgc aatccaagcc aagaacaatt tttctatggc tggattgcta atcgagtatc 84900 aatgtgaagt acctaatttt actgtgcatt ctactcaaga ctgcacagtt acttcgacgt 84960 atagtgaggt agttgtaaaa gtttataggt aagttatagg attgattggt aataatcttt 85020 aaattttagg gggggattgt tcagatgtag tgtattctgt aaaataaaac ttaattatat 85080 tttgctttca atacaatgaa cgatcaagtt ttccaattac aaattgttat aaatggaggt 85140 ttaaccccca ttcaatctaa gccagaaaca cttgataaat tagtaaaaga atttgctata 85200 aatcatttgt tgtttccaaa agaaataact gaacaattaa ttgagattaa ttctcaggat 85260 ggttctcaaa caaaaaagat aactaaattt attgatttgg ttagtagcaa tcaaaaatgt 85320 acctaccaaa ttagaaatga ttcacttgta tttttaaatt cctttgagaa aatagaggaa 85380 ttagaaagta tatttgaaaa gttttttaaa tcgttttcgg atctgacccc atatataaac 85440 tataaacaat caaaaagatt ggggctagtt cttattaggg aggattataa tgaggttaca 85500 ttacgcgagt attgtacttc agaagaatta gatcgaaatg ttattgagaa tagatcgaga 85560 aaagttactc gttttgctat ggcggaacta aatgaaatgg taaatttatc tgtttcaaaa 85620 gattatgtaa ctcatgaatc aggagtttct cgaaatactc tagcaagtgt ttatgatgtt 85680 aatacgttat ctactaagga tgtttttaga tttacaagta aggatgtagt aaagtttata 85740 aatgcttcaa agaaatttat tttagaatca atgtaagatt ttttatatga actattcaaa 85800 taatttatct aataatacta tgtatgaaag tggtaatcat aaaaagataa tcactaagta 85860 cgagattgaa acaataaaaa cggtttggca aaccgataag atacagtctt ttttaaaaga 85920 ttacttaaat acagacgtag ttagtattac tgaccccact gttatagaac aaaagatagg 85980 ggaagagagc ttaaagcaaa ttaaaagaga atttgatatt tttaagaata aatttgataa 86040 ttttctgaga tatgaagatg tgccagtcga ttatgtttca cctattgaaa atgaattaat 86100 caacttttat aaacatagta aagttgaagt tcaagaacaa attagccaat ggattattga 86160 ttcttttgat aacacaaagg ttcttcttaa tattttaaaa attttaggta atattgctcc 86220 tgattttatt gatcatcaat ttttaactaa ttttcttatc gttcttaatc ataaagatac 86280 tgaaatcaaa gaatatgcat taagaattca agagaaatta atgcttccat catataataa 86340 tgtactgaag cactctaagt taactccaaa atggattgat gactatagaa aagaattggt 86400 tgaattgtat gaagaagata ataaaggtag ttaatttaat atgactattt ttgtaagaaa 86460 gataagtaaa gcaaaatggc cttctgaaga ggaaattgca gaaaaagcac tggattcaga 86520 aattatacct tttgtcagag ccgatgcctt aactacttgt ttaaaaactt ctcaaaatac 86580 tttatctgtt tgggcagaaa gattaaaaga aataaaggcg gtttaaccgc cttaaactgt 86640 tgcaagctgt gctgtattaa gcacagtctt gctttgctca tatttcaaaa cgtctttctt 86700 tttatatgaa acacgtctcc caattttcga gaaaggcagt gatgattgat cacaacgcat 86760 tctagctaat gtccaaggcg agcaatctaa ataaagtgct acaacctctt gaggaaactt 86820 ctgttcttca ttagccatta tgaagcgatc caaatattct tgttgctctg catcagatag 86880 atttctcaga tcttttaaca tttactcctc cttactttcc gctttaactt ctcgatcaag 86940 acgctcaatc tccgcaatta ataaagcggc tgcacgaacc aaatcttgtc ttgggctttt 87000 tggtttccag aaagtatcag caaaaggcca ccatcctgca gcatcctcat ccatatacac 87060 ttcggggccg aaatctttgc tgttataaac ccaacctcta cttactacat gatcaacgta 87120 acaagatgca gctcgtggca attcatttgc ttcatagaga ttatcaaatt caggtaaata 87180 cccttctttt ataatttggc gttctctttc attaagaaca tctttgacag cttgactgct 87240 atataattca ctcatccctc agctcccgat tcgcttgcca cttcaaccat caaagaataa 87300 ataggcgtgt aatgatcacg agtcccatcg ccccaatgga atccactatt ttctaaacag 87360 ctaacaacgc tgtctggaat ctctttcggt accaaacagt aaccctctgg caccgcctga 87420 gctttggctt tttctagctc tgcatcacga tgctttgcac atctaagcca agcatcccaa 87480 cgtctattca tgttgcttat ttctttctga gcaatttcag aaggattgtt tgatctagtc 87540 ataaacagtt catgctcatt actaaaaata atgtctcttc ttcctttgta atattggaag 87600 gtattcagaa aagcctctct ttccttattc aaatcaaaca tcattaggcc ctcaaatatt 87660 cttctttagt ccactcaaca aactctttat aaagctgctg cgcgggttta tttaaccggt 87720 tgtgatagtc gatcgttatg cggcgccaag cgactggtac cgcataatgt ttggttagaa 87780 acattgcttg atccatgcct tgccggacta ttacgtagcc cagcaattgc aagtagtaca 87840 taaaaccaag catgtgtttt tggctcactt tcttgtactg atctttcatg ttagaaaccg 87900 tccactaata aataatcagg gggagattct tgttgagtag gtgtaggatt ctctaat 87957 SEQUENCE LISTING <110> Optipharm.CO.,LTD <120> Novel Acinetobacter baumannii specific bacteriophage AB63 and antibacterial composition comprising the same <130> optipharm1-80 <160> 1 <170> PatentIn version 3.2 <210> 1 <211> 87957 <212> DNA <213> Artificial <220> <223> bacteriophage AB63 <400> 1 tgatctttag cttgagtaat caggtcagca agaaagtagg gtgtgcttta agatcaggta 60 cccatacttc accggtttca cccctaaagc acctgagttt tcgcatgatg tgtaggcgaa 120 ggtttgaaat taataacgcg gcatttttac cttcaccagt agtaacagtt gttagataac 180 ccatgacatc tgcgatacgg taaagctcgt tacggttttt accacctaga tctggcggta 240 aataatttga tcaccgtttt gatcttctga tgcgtgtgca atgaaaacaa catctttacc 300 taaactgatc aaagtatgat gtattgcttg aacgtttggt tcgctaaacc ttgagccttt 360 aactttaaag agccatcttt ttgacggtta ttagcagtta gcaatagatg ggttttaatg 420 cattcaagca ttgcacccac ggtatcaatg actacggttt tatatggtgc taagtcctgc 480 ggagtaaggt ttgcaacatc actccattgt tgaacctgta caaccgcacc acgacgtaat 540 tcaccagtac ggtgagcacc acggtcaaag tcaaaagaaa ttgctttttc cgcagtaaag 600 cccatcgatg atttacctaa acccggatca gcgtataggt acacaataat tgcttgaacc 660 aataaagttt ggtcagcagt aataatcggt aacgccattt ttcttatcct tatcttgagc 720 cagtgaagcc gcgcttagtt ttataagcct tgcggtcata agtagggatg tttgtttcac 780 gcagttttat tgcgagctgc tttctgcgtt ggaaatcaat ttcttgtgtg agttcattcc 840 aaacttttgg atagtcagtt tggaacctga acacatttaa aggcgtctta aatccgtctt 900 taactttgta aagaactgag ccattagcat tagatgcgta cacttgccag ccgatgcgga 960 cagagtagag gcccttatca tcacggccta aaaatgactt gtagccgtca gggtgttttt 1020 tgaaattagt catctttaag cctgcaccaa cttgttacgt tcgatgaagc cttttagaag 1080 gccattgatg tttcggatgt cttcaaattc ggtgaaatcg ttatatgact taccattaac 1140 atcagtgatt tcatttactg tgagttgtgt aatatcaaca gcagtgaatt cagaacccgg 1200 aacgccgtag ctgtctggat gagcttcaaa atcaaagcta acgtttaaac ggaagctatc 1260 taatttaatt acagcaacgc cagaatgttt acctgtgatt ttcgcggtta acacaccgta 1320 agtacttggt tgagttttag gtgtaaaaag agtaggtgct tcttttgttt ggaaagctgg 1380 ctgcaattgg caagcaacta aagaacctcc agagattgca agagcagcca tgctgacaaa 1440 tgcaaatgag ttgaaagggg tagcttttac gttcataatt gatctcgcag tttgcaaaag 1500 cacatcagat ttagcggtcg gtgtgctttt ttgttgtcta cgagacaaat atagttttat 1560 ctattattag tgtcaatagg tataactata aaatgatagg tgtaaatttc taaattgata 1620 ggtaaatcta taagtaaaaa gcccacctta taggtgggca aatgtataaa attgtttcta 1680 taggtgaatt tattaaccag ctcgccaaaa ttgacgaccc attaccttaa aattttttcc 1740 attctcttca gtaacttttc gatctctaaa cttttcattg agactatgaa gaattagaga 1800 cccatcagcc tctttaaaaa tttgcttcaa cattccttca ccagcgaagt aaacagcata 1860 tatttcaccg tcaataactt cagtttgaga aatatcaata ccaactaagt caccatcttt 1920 gatgtaatcc atcatgctat cacctttagc tttgatgata cgcatgcaat cctcagagac 1980 acgtttttct ttaaaaaatg atggtggaaa agggattgct ccattgattg catcaaaatg 2040 aaattcaata gattcacctg gtccacatga aaaattagct tccactacgt caatccaaat 2100 gtaattgtct attcctttat gaaccacttt aggttttaca aaatcatctt cggaaaaacc 2160 atcttcagtt tttccagtag ttagccaata cgcatctaca cctaagtatt ttgcaatcag 2220 tggaaggaaa gcagacttca agctcttacc actttctaag gcttgatatg tgggttgttt 2280 aataccaact gcttcagcta cttcatactg acttttacca gctttagtac gagcttcttt 2340 gagtcgagtt gccaaatcag acataacaaa aaccttaatt tgtttggaat tggattttat 2400 aggctcaact ataaaacttc aaaaaggcat acctattgac atatgatagg tataactata 2460 aattaactat caaaatagga gtttgatagt tatgagccaa aatctcatcg caatctatga 2520 aaaattagtt gagttcttag gtggccaaac aaagacagct caagtcctac ttataaaaca 2580 accctctgta aatgcatggc ttaaaggaaa aaaccaaatg tccgaaaaga ttgcattacg 2640 cgctgagcgt gtaactaaag gtcgttttaa agcttatgag ctttgtccat cactttcaga 2700 attcgaaaca ttaaccgctt aggaccttac ccatgagcaa agtatcaaat gaattgcctg 2760 caagcgctag caataacgaa tcgctcatat tgcaagcact taacgctagc aaccaaagac 2820 aagtagcaga gaaggtggga atagatgcaa gcaccttgtc gagaatgaaa aatgacaaga 2880 aaaacaatgg attgacagag attgaattta ttagcttttt gttgacagcc attggtttga 2940 aggttgtgca agagagtgat gtgtattgct cacctgaaat tgcagaagca acgcgagttt 3000 atttagcaca tgcattcact tcacctgaat acatgcggat tttattcaaa taaaaaacca 3060 ctacctgcgg gaacaggagt ggttaggcat tcaaatgagg tggatcaaat gaacacaaac 3120 aatttatcag aacaaccaat cgaactcaac tcaccagatt tttaataggt gacgttgtag 3180 tgcttactaa agagtgccgt actttcaaat caaatgattt gtttgaagtt aaaaataaaa 3240 ctttgactag tttatggact atcaaatcgg agaatcattt gattctggtt tcatcaaaag 3300 aaatccgtac agcaacagta gcagagctca acgctaaacg ccgcctaaca aaagctgagc 3360 aagcattagc ggaggtgtca tgaatagtca atttaagtat aaacctgagt acaaacagac 3420 tcaggaaatt cagtccttct ttgatccagc gttagtgatt ctcaatgagc tacatgatcg 3480 taaccgtaaa aatctaagag ccaaaggtta tgacgaaaat aatgctgcaa taacgcgtga 3540 agaattttca caaactatgg cacagcgttt tcgcattaat cagtggttag cagggcagat 3600 cgttaatagt ttggctaatg ctgacttggt tcaaaaattt ggtgggtatg taaagcctaa 3660 ggtcggtgta catgagtaat tttgtgccta attcctttca agtgcctaat gcatttgttg 3720 acgaggtttt aaataaaatc tctgatgctg catgcaaaat ttatttagtt atttgccgta 3780 aaactcgtgg ctggaataag gagatggatt ccatctcttt aactcaattt gaagagatta 3840 cagggaagag tagaccgaca gttgttaaat gccttaatga attaattaaa gttggtttag 3900 tcgtggaaca accaagcaca attcatggaa atacattcaa attaggtaac gatactagcg 3960 ttggtttaat gcttaaattc cctagtaaaa attttttact acctgaaatt tatggccaaa 4020 ctagtaaaaa ttctttacca ctgctagtta aaaattttaa ctacactagt aaaaattttt 4080 taccgctact agtaaaaatt tttaacacac aaagtatcac tatcaaaaac aactctcaaa 4140 gtaataaaaa ataaataaaa aaagagagcc tgtttctgaa aaacctaaat cagaaaaacc 4200 aactgaattt aatccacgtt tagttgaact accagcatgt gtagatccag agctgtggaa 4260 caattttgtt gatatgcgta tcagcatcaa aaaaccactt tctgaaaatg cagtaaagct 4320 aatccttaaa aaacttatct cgtttggccc tttggctaac caatcactgg aaaactcaat 4380 tatcggaaat tatcagggtg tatttgagcc tcgccaaaat caaattcagg aaaacccaca 4440 atctcataac gttcctgaag aaccgggtta tttcactcaa atgtacgctg agagcaaccg 4500 ttcaaacgtg attgacgtta cgccagtgtc acaagatttt ggaggctatt aatcatgaat 4560 gaattagcac catttgaaag ttttttaaaa gaactaattg cggcttacag aactaaatac 4620 gctgttcagt tcaataagaa ttttccagta gaggggaaaa atgccgttcc aatgcaaatc 4680 gttgaacagc agcttgctaa agcattggtt ggggttacac ctaaccaact tcaaagaggc 4740 ttagcgctat tttacgcaag tacaaataca tacatgccta acttcgctga attccgtgct 4800 atgtgcatgg gtgatgattg gtggagcgct gagaaggctt gggttaaggc ttgtgaatac 4860 actcagatct ctcaacacaa aaaagtgaca ttgccagacg gaagagagca gaaccaagaa 4920 attacaacct tgaccaaatt tgttttagac caagtttatt cactaatcca agatggtgaa 4980 atgtacaaag ccaaaatgga atttatcaaa atctatgatg aatacaaggc tgaagcacaa 5040 ctgaagggta aaacccaagc ttggtaccaa gaaccaattt tattagctca gaaaaatgag 5100 caaaaggtgc ataagcctgt ttcaaatgac gaagcgcaaa agcatctcca atcattgatg 5160 gaacgtttaa aaatcaatgg tcgtaaacct gtaccagtac aaaagcttaa ggctaaggaa 5220 aaagagccag aactcaaaca agagctaggt ccagatcctt ttgacaatcc gcacgaatac 5280 gcagagatgt gccgccgtga aggtatgcca attcctagaa atattcttaa gttaattgat 5340 ggggcgaata tatgaataaa ttcgagattt tagcgtgggg tttactcatt tcatttttta 5400 cagcagctat tagcggtgcg gtggtttggt ggtggttggc gcgtaaagag cttgatgaga 5460 aaggagccag ccatgaaagc aactaaattg attagagata aaggactgca atacgcgaag 5520 gaaatcgtag attcagcacc cgataacgca actgaatgga acgagggtta tgagttccaa 5580 tgtggtcaaa gtgtagaaat cagcccagca gatcgtgaga agtattttgt agatttggtt 5640 gagcttaaac gtctggtgga gtctttgaaa atcatcaacg atttaggtgg agttgagaag 5700 ctaacgcctg cattcattac gacagataag catgttggtt acacgcatgt tcgcatggtg 5760 ggaaatggga gattgagctt tcttgatgat ttttgcgact tcattccaga tggttccatt 5820 tcaattaagc gtgtgatgac tgctatccgc gaccacgaat caatatacgg aggcggtgaa 5880 tctcatgcca actagatata acacaggcga gtatagctac gatcttgaat atcactatgg 5940 agatatgtca gcaagcatgg agatgcttag agcacgttta attgaattgt tgactcctca 6000 tctgtctggc cgttatgtga aatggagaga agcatatttc acatggttta caaagtgcgg 6060 cggggattcg gggtggatgt tttgtgtagg tccacacgaa tttcatattg atggggcgtt 6120 aaggcgctat tactcaggtt ctattgatat tacctacaac cagaaagatc gatgtttctt 6180 ggtgggtgag aaaaagaaag tcaaatgtaa ggcttgtaag gggtttggct tcattcgaga 6240 tgatgggtgg gggcatatag ataaatgtga aatgtgtgat gcagaaaaag gagccagcca 6300 tgagtgagtt tgagggtaaa tctggaaagt gggcttggga gattcaaaaa gaacaacaag 6360 cgaaagtgga ggagctgcaa aagcgtttag atggggcatt aaaagagact caatatgctt 6420 tgcagtatgt tgaagaagac atgcgcggca atcatgaatt tctacaaatg gcaatgattc 6480 gaacccttaa agctatagag caagtgctca aaggtggtgc ttgatgtcat cagtcagcat 6540 tgctgaatac cgcaagttat ttcccataaa gaaaaataaa aagcgccgtt cagcaaagca 6600 agttgccaga caaccaagtg tgggtgaagt ggttctggca acgcatttaa gagcatgcaa 6660 gattggattt gaacaggaat ataagttcca tcctgaacgc aaatggagag cagatttttt 6720 aataaagggt tcaaagattt tgattgaggt agaaggcggg atctggagcg gaggccgtca 6780 cacaagaggc aagggctatt taggggatat ggagaaatac aactccgcag caatgatggg 6840 ttttacagtt ttacggttca gtacagagca agttaagtcc ggtatggcat taaagcaaat 6900 tgaattatta attaagggta aataggaagg cgattatgtt ggttgaaaag tttgatttta 6960 ttgagttact tcgccttgct attgctcaaa gcgaaggtaa agggaaaatt actaagcatg 7020 ttgttttggg agaaattgcc ttattgcctg caggtgcaaa aaaatgggca gaattactgc 7080 ttgaacgtat tgattttgag cgcattgcag aaatcacaga aacaaagaaa atttatgaga 7140 ccaggataat taatggtaag gaatcaaaaa agcgtattgg tgaaataccg ggtaaagttg 7200 aaataaaaaa aggggagatt aactcagctg attttttccg cgttagaaac gtactagcgg 7260 gtaagatcca tcgtgaaatg atcaaaaaga actttaagcc aaataattgt cagggcgatt 7320 tatcaaatgt ggccaaaggt attgctgagg ttgttttgcg tgggcgatta tttacaaagg 7380 caatgtgtgg ccattgccag ggattaggca aattggagtt atttaatgaa aagggatatc 7440 caagcggatc taagttttgt gataaatgca gtggtacggg gaaacgccca tatacattgc 7500 atgaaaaaat tacgatcgca aaattaaaag tgtctaagtc tggttattct gagcgatatg 7560 aaccatatga attaattgct gaagcatgta tcgaaaattg ggaaaacacc attagaacta 7620 gcctggctag atcgtttcat tttgaacctg aagaaatatc attagcttga cttaaacaga 7680 acggttgagt ataagtattt ctaaaatggg cgctttatac atggatcgcc tgaaaaactt 7740 aataaaagct cactaatttt agtgggcttt ttgcgtatct agagcattga aaatggaaaa 7800 cacctggcat gctgaccaag aaaaaccaga attacggcca gatgaaaaac ctttgaattg 7860 cccattttgt ggatctgatt caatttgtac ggattcttca cattatggaa aaccagatga 7920 agacggctct atagcgtggg atgctttcac atggtgtcat gattgtggat caaaaggccc 7980 tagtgcttgg gcgatgatcg cttgggatga aaattttcat tacgacactg tttatgaaga 8040 aagatcaatt gttaattatg ctattcgcca gtggaataca cgcaaataag atttttaatc 8100 tcgtgagggg tgttttataa gcacacctct cttttagccg gacggattac ggcgcaaacg 8160 gcccgctaca tactagttat tggcggggct ttttcttttt ggagtatgta tgactgaatt 8220 tcaaaaaatt acgcatgaga ttagacagct ccaagtagag ttaaatcatt taggaagctg 8280 caatacaaaa gacttaacag cagaacagat cgctcacata gatgagcgat tttttttggc 8340 cattgaaaag caaagtaaat taattgctcg tctcaacaat aagccagagg gcttcttata 8400 aatattggtg gtgcgatgga tcctaaaaag tactatatgc tcaccaggaa aaaagaaaga 8460 aaacccaagc caaagagcac accattacca aaagccaagc aaaactattt agaggccgaa 8520 gcaactctaa aagaagaatt aatcgagttg gccattggtt ttgaaagtaa gtttcagccg 8580 atccatacca aacactggcg ctttgatttt catattgtga aattgcgttt gctcattgaa 8640 attgagggtg ggccctggtc tggtggacgt ggtggaaagc tggcaaataa agcatggagt 8700 cttgatcgat atgatcaagc tgaagagatg ggttataaaa tagagcgctt tcatccagat 8760 tctattttgt cgggatatgt catcaactgg ataaaaagtg aattagcgag aattgaagat 8820 ggagcaaata agaccatttc caccgactga ttttattgat caagcagatg aagaagaagc 8880 aattagacta acaccagcac cagatctaaa aaaatgggtt gttgctaatt acttaactat 8940 tggtggacct ctttataacc ccgatcatga tcacataggt gaactgcttc acgataatga 9000 agaattttta gcattcgcgt gggcctcttc tgcatataaa agcaagcaag ctatggtgtt 9060 gggccagtgc gaaaaagtca tgttcaatgt cggtggctgg cgtaaagctc gacaagagca 9120 acagatgcga gactggttcg gctttgtgcc aacttactta ataactgtcg acgcttcttt 9180 ttgtgagcgt gcaaatgata cagagttctg ttacttgctt gaacatgagc tttatcacat 9240 tggagtgatg agagacgagg acggagaaat tgtttatagc gatagttctg gtcttcctaa 9300 gcactatctt gcaggtcatg acgttgaaga gtttattggc gtagttaaac gttatggccc 9360 aagcaaaaat gttaagcgac ttattgaagt cgcaaagaat ccgccgtttg tttcgaatct 9420 tgatatttca aaatgctgcg gaaactgtgt aatcaattga gcctaatggc tctttttttt 9480 gcccattttg ttatacgtag ttatacgatg aggaagttat ggcgacacta aaagagcctg 9540 tgaaaatctt tatagttcag tctcttgctt gtcgtgatac acctcaagaa gtggctgaac 9600 tcgtaaaaca agagtttggc gttgatatag atcgtgttca agttgcaact tatgacccta 9660 caaaggttgc tggtaagaac ttaagcaaaa agtatgtcga actatttgaa aaaaccagag 9720 atgagtttga taaaggctta attgatattc caattgctaa taagtactac cgattgaagc 9780 aataccaaag acaacttgag aagactagaa acgtcaaaac agccttaaaa attcttgagc 9840 aagccgctaa agacattggt ggtcaattta ctaatcgcca agaaattaca ggcaaagacg 9900 gcggaccagt ccaaacagtt aattcagaaa ttccagttcc aatggaagat tacttaaaag 9960 cgcggaggga agtcttagat gagtactgat gcggctcggg ataaagccat ccggatcgag 10020 gcgcaagaag atttatattt cttcacaagg tacatgttta aggagcgccg tggttataaa 10080 tggatgcaaa attggcacca cttagaaatc tgcgaagctt taatgaaagt ttatcgcgga 10140 gagataaagc ggttaattat taacgttcca ccacgatatt ctaaaactga aattgctgta 10200 attaatttca tggcttggtg ttttggtaag aatccagact gtgagtttat tcatatcagt 10260 tactcggcaa tgcttgccgc aaataatgcc ttccaaatac gaactcttgt acaagaagag 10320 gcgtatagaa aagtctttcc tgagcttaca ttgcgtgatg atagtaaggc taaagacttc 10380 tggagaactt ctcaaggcgg tgtctgctat gcgacaggta caggcggtac gattactggt 10440 tttggtgcag gaaaacttcg taaaggcttt ggtggctgca ttattattga tgacccacat 10500 aaagcacatg aagcttcatc aaaaactatt cgagaagggg taattgattg gtttcagaac 10560 acactcgaat cgcgtactaa ctcgccagat acgccgatca ttgtgattat gcagcgactt 10620 catgaagatg atttagctgg atggttgcta ggtgatagaa aagacggcgt tcctgtagct 10680 ggtggtaacg gtgaagtgtg ggagcatcta tgtctttcag ctattcagga agacggatcg 10740 gcactatggc cagcaaaaca caatattcaa aaattgagac taatggagca agcggcaccg 10800 tatgtatttg ccgggcagta ccgacaaatg ccatcaccgc cagcaggcgg tttttttaag 10860 cctgacaata ttcaaattgt tgaggctttg cctgcagatg tattgaaaca agttagggct 10920 tgggactttg gggcaaccga aaatgaaggc gactttacag taggtgtgcg agaagctcta 10980 ggcgcagatg gttttactta cattgtcgat gttacaagag gacagcttgg tccagacaat 11040 gtgaataagc gtttagaaca aacagcaaag ctagatggga aaaaagtttc tgtgcgttta 11100 ccacaagacc ccggtcaagc aggcaaatcg caagctagtt catttgtgaa gcttcttgcg 11160 ggttatagcg tgatagctaa gccaatttca ggtgacaagc ttacacgtgc acaaccattt 11220 gcggcccaag ttaacgtagg aaatgtacga atgctcaaag gtgaatggaa taaggatttt 11280 attgatgagc ttcgtcattt tcctaatggc acacatgacg accaagtgga tgcagcttca 11340 gatgcgttta atgaattaca tgaaggtttt gaagccttct ttgctgatat gggatttgct 11400 cgatgagtga tgtaactttt caacatgctg aatatgttaa gaacttgcca tactggcaaa 11460 aacttgatga tgtttgtgaa ggtgaagatg cagttaaggc taaaggtgaa aaatatttgc 11520 cgatgccaaa tgcacatgat aaatcacctg caaataaaag cgcttatgag gcttatctta 11580 cccgtgcagt cttttatgaa gtaacaggga ctacattaaa tagtttagtt ggtgcagctt 11640 ttgcaaccga tccaagtttt aaatttcctc cggaacttgc tcatttagaa cgtaatgcaa 11700 atggtgctgg tttaagtact tatcaattgg ctcaaaatgg aattcgccat ttattgaagc 11760 attatcgttg tgctttatat gtagattatc ctgatgtgcc gccagctcgt aatctagcgg 11820 aatttaaagc acaaaaagcc tatccgatga ttcatttact aaatgccctt gatgtagtga 11880 attgggattc agtaatgatc gataaccaga aaaagctttg cttagtggtt atacgtgaat 11940 ttaagtctga gcgcggtgct gatggattta gtaaaaccga acaagagcaa tatcgtgtac 12000 ttcgtttaga gcaagaggga aatggggaat atatttattc cgttcaggtg tacacaaagg 12060 gtgaaaaggg taactgggtt ggcggagaga agaagtttcc aacagattac aacgggaatt 12120 tctggaccta tatacctttt acatttgtag gtgcaattga taattcagaa gagattaaaa 12180 agccaccatt acttcctttg gctaatctca atttagccca ttacagagac agtgcggact 12240 ttcaagagtc cgttttttat atggggcaac ctcaatatta tgcgaagggt gttaattggg 12300 agtggtatga ccaagccaag aaacgtggca tctacattgg agcgaaagta cttttgcctt 12360 tacctgaaaa tggtggttta ggaattgtac aagccgaccc taatactctt gcccgggaag 12420 cgatgaaaga taagtgggaa aaaatgaagg agatgggggc gcgtttaatt gagaagggct 12480 cgggaagtaa aaagaccgct accgaagcga atagtgatga cgccgttcag cattcagttc 12540 tttcgctctg tgtcgttaat atgaatgaag ccttgtcagc agcattacga tgggctgcta 12600 agtttgtaac gcctaatgtg gatgttctaa ctaaagatga tttgatgttc gaaatcagtc 12660 aagaatttaa caaacagggt tatttagctg agttagctcg acagttattt gaagcagctc 12720 tacaaggccg atcttcattt aaatcatggt gggaatacaa ccaaacaggt atgttcccta 12780 aacaaaaata tgaagaagag cttcagaatg ttgaagcaga gcaagatggg actttaaatc 12840 aaaaggtaga gtgagatggc aacagatatc aaaaaactat ttgaagtact cactcagcac 12900 caggcctatc tttatcgtgc ttcatcaaaa acggtaaatg agttattggc tttattcaat 12960 gatgatacga gcaagatgct atctaagctt cgggatttat tggatgagct taatgagtcg 13020 gagaaagttg ctttagctgg tggtaaatat acaacttcaa atttaaggga aattagggat 13080 ttgattgccc aatggtttgc cagtgttaat ttagcattac ctgaagcttt tgccgtttct 13140 gctacggcgc tggctgttta tgaggccaat tacgtagcta agctctatgg agcaaaaatt 13200 aataagcctg atggggaaaa actattctta tccgctaaaa aagttccgtt ggcaggtggc 13260 gctcttgtcg atgatctgct ttcaagaatt gctgaaagtg cccgtcaaaa ggttgagtat 13320 gcaattcgag atggtattaa ttcaggcaaa actaaccaag aaattgttca gcgtattcgt 13380 ggtaccaaac ggcttaacta tgaagatggg atcttaaatg gtaccaaaac tgatattgag 13440 cgaacggtaa gaactgtgcg aagtcatgta gctaatcaag cctatctaaa tagcttcaac 13500 caaattggct ttgaatatgt ccgatttgtt agcgttttag atggacgaac ttctaagctt 13560 tgcgcttcat tagatggttc agtgtgggaa ataaatgatc cggcaaagcg agtgccgccg 13620 ttacatccta actgtcgcag tatcttggtt ccggtcgaga aggacggtca acttgttggc 13680 gaacggccat ttgtaatgga cgaacgtaga gttaaagaca tccccaaaga agagcgaagc 13740 cagttaatag gacagttaga tgcaaacacc acattcaaag agttctttaa gaaaacagat 13800 gatttctttc aaagggagtg gctagggcca aagcgcttta agctctataa agatgggaaa 13860 tttgattttg ataagttctt tgatcctgaa ggccgtttct atagcttaga tgatttgaga 13920 aagttggatg aaaaagcttt taaaaagttg ggtctgtaat ttttcttatg ttatattttt 13980 taaaacatca gaatttatac aatatgaaaa caatagcttt tgtatgtcta accctaattt 14040 ccatcacttg tttagctgaa ccaagtcaaa aatatcttaa agaatatgat cgattgtctg 14100 aagctttgga gtcagcaatg gcaaatgcat attcttttga tcctgcaact ggtcaagtaa 14160 aacaggctac tcaaggttta gaagctaaaa ataatttatg tagagctgcc caggcgaaac 14220 taaacctcac cacgttttta aaagacaatt tagaggaatc taaagagctt tataaatcta 14280 ttgatggtgc agagactcta gataaaaatt atcttagtgg acaacagcag gaacaacaaa 14340 atctcgtttc aaatttgaaa aaagaccttg ttggaactgg atttaactgt gagtaattat 14400 tgccgattac aggtaattct aaactcactt aagacacaat tttcacctat ataagcgccc 14460 aaatggcgct tttgtcattt atggagtttg gcttatgagt gaatcaaaag ttagacattt 14520 ggtacttaaa agagtttcag ataaatcttc tcatcttgct ctttgtgacg aggaaacagg 14580 tattccatta gctggattaa ccgctgtaaa aatgaattgt agtgtttttg agggtccagc 14640 gactatcacg gcaacatttg atgtaggtgg tcctcaaggc atccgcttag ttggtgatga 14700 acctagacaa aaggtttgga gtgcaaagga aacgtagcga aaggcactac aaatgcctga 14760 aaagcaaatc aatatgtcag atgctcaata tattctgagc acaaaatgaa ttctggtgcc 14820 atttcttcaa attaaggttt caagccatgg caatttatgg ttttactttt gaaagattaa 14880 aagcaattgc actcatcaaa tagaacttaa tttttaacca tagcaccttc gggtgctttt 14940 tttgcgagaa gaaaatgcca agccctatta tccaatattt ccaatatgaa catttacctg 15000 aacatttgca gcaagttagt aagccaattg gtgatttagc tcggcaaatg gatgagcaac 15060 ttcctgacgg gcctgaaaaa tccacaggat taagaaagct acttgaagca aaagatgcat 15120 ttgtacgcca agctttaagt aaataatcat ttatagaaat gaagcgtcct aaagggcgct 15180 tttttattgc ctgccgaaag cggatgctaa cggcgaatcc gggcggatgc ccattttgta 15240 tatataggtt ggatgaccaa tgaaacttaa aacagtaaca atcgacggta aagtttatgc 15300 ggaagtagac ggtgataagc cgatctatat tcatgatgac ggcaaagaaa tgccacatga 15360 tgcaccacac tcggtagcaa caattgcacg cttaaacaat gaagctaaaa cacatcgtga 15420 agccaaagaa gcagccgaaa aagcattaaa agcttttgaa ggaattgaag acccagcggc 15480 agctaaaaag gcattacaaa caatccaaaa tctcgatgat aaaaagctgg tggatgccgg 15540 tgaagttgag aaagttaaag ctgaagctat caaagcagtt gaggaaaaat atgccccgat 15600 tgttgcgcaa cgtgatgctc tagaagcctc tttacataaa gaacttatcg gcggtggttt 15660 tgctcgttct aagtacattc aagacaacat tgcagtacct gtggacatgg ttcaggcaac 15720 ctttggtcat cacttcaaaa tcgaagaagg caaggtggtt gcatatgatc cgaacggcga 15780 aaagatttat tcacgtgtcc gcccgggtga acttgcaaat gttgatgaag ctttagagtc 15840 attggttggt ggataccagc ataaagactt aattcttaaa ggtggtaaag gaactggtgg 15900 cggttttcaa ggtgggggca aaggtggagc acctactgga atgaaacgca gtgaaatgtc 15960 tgtttctcag aaagcagatt acatcaaaga acatggcaat gatgccttcc taaaactacc 16020 gaactaatca ttaaatattt ggagataagt agttatgact acgacagtta attcagacat 16080 gatcatctat aatcaattgg ctcaaactgc ttatttagag cgtttgcaag ataatttgaa 16140 tgtatttaac caagcctcta atggtgcaat tgtttatcgc aatgagatca ttgaaggtga 16200 tttcaataaa gaagcattct acaaagtggg cggtagcatc aaacatcgtg atgtgaattc 16260 aaccgccaaa gtagttccag agaaaattgg ttctggtgag tctgtaggcg taaaagtccc 16320 atataaatat ggtccttatg catcaactga agaggcattt aagcgccgtg ctcgtacacc 16380 agaagaattt gctatggttg ttggttacga tcttgcagat gcattggttg caggccgatt 16440 agagtacagt ttagcttctt taaaagctgc tatttctagc aatccagaca tggttgcaaa 16500 aggtagtatc gttgttgatg gccgcaaagc attgactcgt ggtatgcgaa agtttggtga 16560 taagtttggc cgcattggct tatgggtgat gaactcagat acatatttcg atattgtcga 16620 tgatgcaatc actaagcaaa tttatggtga atctgaaatc gttatctacg gtggtttacc 16680 gggaacctta ggaaagccgg tcttggtgac ggacgctgta ggtgataacg atgcttttgg 16740 tttgcagtat ggcgctgtca ctgtaactga atcacaagta ccgggcttcc gagcttatga 16800 catcaatgat gaagaaaact tagcaatcgg tatgcgtgct gaaggtgcat ttaacttaga 16860 tattcttggt tatagttggg atacatcgaa aggtgaaaat cctgacctta cattacttgg 16920 ttcaagcgct aactggatca aatatgcaac cagcaacaaa atgacagcag gtaccttact 16980 tgatttatcg ggtacagcga caactggtta aaacctaaaa attaaaacct aaggggctaa 17040 taagccctct tttttattat taagagaaaa gcgccatgaa gattatctat acacgcattg 17100 cagcagcggc tgcattagag acaggcatta ttgctaaccc tgactattat gaaaacccaa 17160 atttgaaagc aaaagaggta attatttacg gtaattatcc aaagattcaa aaggattatg 17220 aatctttgga agttccagtt gaagttcgta agttggaagt gccacaaaaa acgactttgg 17280 ccacagtaaa tgtcgcagtg ggaattaccc ctgaacttca agctgtgatg gatgatgcaa 17340 aagctgaatg tgaaaaggta gttgaagaaa acactcagct taagcagaaa attgccatct 17400 tagagcaggc cggtggtaac cagtcagagt tgttatctga aaattcacga ttaaaagatg 17460 cagcagtctt agcagataaa gctctcaaag atgctgaagc tcaagtggtc ggtataaaaa 17520 ctgaatttga agcttttaaa aatgatattc ctgcaatgca ggcacgtatt gctgaattgg 17580 aagctggaaa agcggcagaa aacccagcta cagaaacggc agctaatgat tttgaaaact 17640 ggtcaaatga tcaattaaaa gagtatttgg ctagtaaaaa cattggttac aagccgtctg 17700 caacaaaagc agaacttctt aaattaatcc cgaaggaata atgcaatgag ctttattact 17760 gtagatgacg caaattcaat tttgggcagc gattttgcac cagacagtga taaggctcgt 17820 ctggtgaagc tggcaaatgt gtggatgaaa aacagaattg gttttgtacc agatccaatt 17880 gatccacttc ttaaggacgc ggcttgtgaa attatcaaag gaattctggc caaagtaatt 17940 tataacggca aagagcagca gttgaagcgt aagaaagtta aggctgattc tgttgagtca 18000 gaaaaagaat ttcaagacgg atctgaagca atctctagct ttgaacagat agcaattgat 18060 tttattgatt cacttgattt gaaagatcca aatgcaagtt ttaatggctt tggcatacca 18120 ctttacaggg catgatatgg gcttacgtga cgaaattcag gcagacattg ctgaagcatt 18180 taatgatgat ttagcagatg ccattcatac ctttacatgt gagcggatct ctaaaacgaa 18240 ttgggatcct aaaactgaaa cttatgttga agttaaagaa aactattctg gccgtggcgt 18300 tctgtttggc tcatacagtc aatatgagat ccaaacactt ggagtactgg ccacggataa 18360 aaaggcaacc gtgcttcaaa atgaagtaac tatgactcca aaaattgaag atgaatggtt 18420 aactgcctta ggctcattcc gggtaattaa tattcaacag gatccagcta atactatttg 18480 gaaatgtcag cttcgaaaag tgtaggggct aaaatggtta atcctgatta tgttcctgaa 18540 tggtatatct cgccttttca acatgtgcag tacacgcttg ctcgaaatca actacacatg 18600 gatttgttat ttgaagatat ggataaggcc gatcaatttt tggatatggg agcggatgcg 18660 caagttagta ctttttctga tggtgcatat gcaatcgtcc aaattggtga tacggcggat 18720 aaagaccgaa ttcaagttta tggattgctt ttacatgaag ctgttcatgt ctggcaaaag 18780 attaaaaagc tcatgggtga acgagaaccg agctctgagt ttgaagctta ttcaattcag 18840 gcgatcgctc aagacctttt taaaatgtat gaagaaagcg aggtgaatga tgggatggaa 18900 ggggaaaaag ccaactgaat ttagttttga tgtgtctaaa gcagcagaag accaggtgaa 18960 gcatattgtt atggatactg tgcaatcctt agttaattta agtcctgttg atactggagc 19020 ataccgtgct tcacatattg tttcggttgg atctgctgat ttcggcgtgc gtgaacctga 19080 aacaaaccct attaacgacg cagcgattca ggcaatgaag attaagttag gtaatttggt 19140 ttatatccag aacaataaag cttatggacc gcgcttagaa aacggctggt ctgatcaagc 19200 accacaaggt atttatggcc tcacgtttaa ctttatttct caaaagtacg gtggctaaaa 19260 tggcaatgac tttagagcag acaaggcaag ctattattga tcgcatgcaa agctttacgg 19320 gtattgctca agaacggatt cagtatccaa atgcacctga ctttactgtt ccaacaaaag 19380 gtgtatggtg ccgtttaacg attgcaggtg gcccgagttt tacctcaggc attgcagata 19440 agccatgtac acgccgtacc ggtaatatca tgatccaatg ctttgaccga ttacatactg 19500 gagagaaggc cgtaacggtt ctaagcgatg cattgctggc tcattttgaa tatttcacaa 19560 tcgaacactt agaatgtttg aatggccaat ctatttatgc gggtaaagat gctgatttca 19620 ttcagtataa tgtgagcatt gggtacaagg tgaattgata tgtcatgtat gctgacttta 19680 gaagaaatcg aaattaaacg gcaagaactg gaacggcatc ttgaagatgt tatgtctgtt 19740 gagttgagca aatggcaatc tgaaaacaag ctatgtgttt ctgatgtgaa tatacgcttg 19800 gctaatgttg ttagtctcgg agggcctaaa cataacgttg ttactggagt aagtgtcgat 19860 ttagataatg agctttgagt tcaagaaaaa gctactgcaa ggcgattatt tttaatgacc 19920 tcagcatatt atcatttgtg attacattct gttacagtaa tggaaattta taacaaatgg 19980 taaaacatga aaaaatcaac tttaggctgg ggtgccgcag gattagtagc tttagggatt 20040 tttggttcag gcaatgataa ctctccaaaa caaacttcag actcagaaaa tgcgcagagt 20100 gcagtagagg aagttatcga atcaaaatat atcaacacta attctttaaa tattagagat 20160 aaaccaaacg gtcaagtagt aggaaagtta ggacgtgggg aaaaagttga tatttatgag 20220 atgaaaggaa actgggcacg tatttcctta aattcctcat cacctcagtg gttatcaaca 20280 aagctattat gtgaaacgga tggctgtttt aaacaaaagt ctcgatcaac cacgtcaaat 20340 aattatcagg ccttaaaatc tcatcctcat cattctgaaa gaaaacagaa aaaaacctac 20400 tacgatagtg attgttcatg tgctgtggtg gattattgcg tgggtcctag aggtgggcac 20460 tactgtatta cgagtggagg aaacaagaga tataaaccta gatattaatt aatttgaatt 20520 atgagacctc cattttgaga ggtactttat gtcttaatca ctaccacctc atcggtggtt 20580 tttttatgtc tataggaatc acttatgagc aattttgtat ttaagcgtgg tgacactttc 20640 aacttaaatc ttcagctagt tgatatggat gaagccctgc aatatccacc agatgatgtg 20700 cgccgtgcaa ttgatcttac aggttatacc ttcacttcac aggttaaagc tctggctgat 20760 ggtgctactg tggccacatt aacttgtact gcattaagcc aaagcacaca gaaagggtgg 20820 ctgaacatta aatctagtgc aagcactgca acttggcctt tagggctgtg tcagatggat 20880 attaaagctg tagttagtgg cactacacag cacactgaaa ctttgacttt ccaagtgatt 20940 gacggagtaa cagcataatg gcaaatcttg tttttaaatt tagttgggat catcggccat 21000 tcccatataa cgcctcacag ggcaagcggc agtttatgtt gccatttgcg tcaggtattc 21060 ccaatctggc acccaacttt tctcaagtag ttggtactgc agctatctct caaggtggaa 21120 cgggggcaac tacagcggct ggtgcacgag ctaacctagg cgcagctgca agtggggtaa 21180 atagtgatat tagtgagctt aagggactta caaccccttt atcaatttct cagggaggat 21240 taggagctga taatgcacag acagctagaa tgaatttggg gttaggaact gctgctgtac 21300 tagcgtcaac aacaagtcaa tatgatccta cgccgggacg agcactaaga gtcggtgatt 21360 gggggatagg ggctgaaggt tctcgtgtat ctgatatggt tgctcctctt aataatggtt 21420 tttttcgaac agatgacact ttaacaaatg atactggtaa tagtattggt ccttatggtt 21480 tctttttaca ctgtacccga cgctcaatgg gtttatatac aaatggaagt cattcatttc 21540 agcttgggaa agctgcctca tattctgccc tgaagtatcg atttaataat agtggtactt 21600 ggtctaattg gtttaattta ttgactgcac aaaatactac aactgatgga aatggtttta 21660 ttaaagccgc ttcaccagtc gttaagcttt tccaaagtca tattgagcta aataacgatg 21720 ctgccaagca accgatcact tttgacaaat taggcactgg tgactatctg attaagggct 21780 ctttaggctt tgcacaggaa ggttggtata tcgaagtacc taaggatgcc aacggtaata 21840 cggtagtagc agttgaatat tcaaccttag aaaatggtga tctttctatt aaaacttata 21900 aacgtaagtt tgatgtggaa aaggcagcca ttgtagctga tctcgaaaat ccacttgata 21960 ttccagaagg ccgctggatt gatatccgtc tgcatgaaga acctgaacca gagcctgaag 22020 agccgttgag tgaaacacca gtggatttcc agccgactaa cttatctcag gcagttgctg 22080 cagccatgaa tggcgtggaa ccgccagaaa tctcagaaac agacgaaaca ctttaataac 22140 ccgcttaaac agcgggtttt ttattgccta aattttggag aaccataaat gagttcaggc 22200 gcaaaaattc gattatatgc ttgtgaggaa gcagttttag gaacaactcc tgcaaatcca 22260 gtctggtaca ccgttcgccg tgttactgat agtttgactg aaaatgttac tactgaagat 22320 agcagtgaag tagttgattc acgttttcgc caaggcgctg ttgtaacgga agccgaagta 22380 actggtcaac tagagtttga attatcacta ggtacctttg acttattctt aaatgttctc 22440 gctttcaata actgggctgc aaatgcttta agttttggtg gtggagtacg taagtctctt 22500 accttggtaa aagtctttga agatattggt caagtcttta tttatcgtgg tattcaagtg 22560 aatacaggtg aaatgacgat ccagaccaca ggcaaaatca ctggtaactt tggtttagta 22620 ggtagctcat ttacgcgaca gcaggttaat cctgttacaa atcctattcc agcatcgact 22680 cgccctctgg tgagtatgcc aaatgttgaa aagctactta ttaatggtca atcaattcag 22740 ggtaaagctt gtctgcagac acttaccatc aactttagta ataatctgga agcgatccgt 22800 tgtatcggtt ctggtaagta cacgcctgag ttttacttag agaaaatgat ggatattggc 22860 gtaaatgcta atttcatgtt ttcagcaaca tctgcttctt ggattgatgc tattaaaacc 22920 cgtgatgtat ttacattgac cttcgatatt acagatacca aaggcagtaa gtactcgttt 22980 aacttcccgc aacttgaagt taaggaagca aatcaccctg atggtggtgg cgatgacatt 23040 attacaatag atatcaattt tgcccaagtg cgtaccagtc caacgattgt acgtgctctt 23100 gtgtaatcaa cttattcagt aacaaagcct atggaaaccc atgggctttt ttatttctaa 23160 aaattagagg ttgttatggc tttaaaagtc ggaattatta aaagctcaga cgtatcaaaa 23220 tggtgtgaat acaagggtgc tgatggcgat gtacaggctg agttcaaagt ccgtggtatc 23280 gcttataagc cttttcaggt agctattgaa cgggcaggaa accagatctc gtctaaaggc 23340 tatgatgtga tggtcaaaga tgaagatgcc aagctttacc acgagctttt aatggatgca 23400 tgcgcggccc acttaatcga agactggaaa ggtgtggtat ttgccgaaat cgtagacggt 23460 aaaactgttg agtccgaaaa gccatataca cctgagaatg cctcaaagct tcttaatctt 23520 ggtgatattg gtatttcaat ctggctattc attaaagaac aggcccagaa gattcaggaa 23580 gacgcagaca aggacaaggc tttaattctg ggaaagtcat ggagctctac aaataccaaa 23640 aaacgtatgc gtcgaaaacg ccgcacgaaa tcgagcaaat caagttctta ggcggccgta 23700 ttccggatcc gccagaatat tcgtatgcgg ctgattcaat tctttcggca tttagcacta 23760 tatgtcgatc cagacgttat gagcaaagca taccgttatc tttagatcag caggctatca 23820 atgtctatgc tgagcataat gatttgccag tggctgctca tatttttaat gactgtattt 23880 ttgcgttgga taatttgttt ttggaggagt gccataagaa gatatcaacc aaaagcaaag 23940 gtaagtgacc aaattaggta ttgccaggga ctgaaaagcc taatttggtc aaaacgtcaa 24000 acaattaagc agttgctctt aaacgcgact caaaataacg cagtcgatgt tacaaaatac 24060 ttgatctgga ttgacagaaa attaccttta aggtgttgcg cgtgattatc aaatgatgaa 24120 taatcacctt accgtcaata tttgacggtt cagcattctt ttactctttc caagaacctt 24180 ggtgtttgct tgtatgtgtt taacattaac tgaagctaaa caaaaactta gagcatttgc 24240 tagagatact agcaaaatca agttaactgc acatgcaaaa gaaagaatga aagaacgctg 24300 tatctctatg aagcaaatta tttgctgttt tgagcatgga gatattactg agggccgtac 24360 ccaaatactc gtggtgattg ccagttaaat gtttctgttc gcactgcagg cgaatacata 24420 acaacagctg ttgcaatcaa gcagagcgag aacggtgaat tctcagtagt agtcactaca 24480 tttagagagt aggctaaatt atgtatcact atgaagaatg cggtctgagc aatatttggc 24540 tgcgcaatgg atttacaatt gaaaatgatg aagactatgg tgaactcgta tctattgaat 24600 ctgttcatga gcttcataat gccattgggt tgttcttaat tacgcaaaag cctgacttga 24660 atggtgagga aattcgtttt ttacgtaaag aactaaactt gtcacagaag aatcttgctg 24720 ggcttttagg agtcagtgag actagtatta gacattggga agctgatcgc ggtttaattg 24780 gtaaacctac tgagctatta cttcgtgcat tatataaaga gcatgttcaa ggtgatggca 24840 aactaagaag tatgattgag tcattaaatc atcaggaacg aactttagta ccaagtgaaa 24900 ttagtttttc atatggaaat aaccattcat ggcatcaaac caattgtgaa atagcttagt 24960 tagttttatt tgatagaaac caccttcggg tggttttcct ttatgtgaca tttagtaacc 25020 agtttgttaa agttagtaca ctttataaca aacggtgaaa ttcatgaaaa aaatattggc 25080 tgcgggttta attggtcttg ggttggtggg gtgcgctact ccagcctata attatcaagc 25140 tatacctaaa aatataagca aaccgccaat tggatcagtt aataaagcat ttgtagggga 25200 tcaaatgctt gaacagggaa tggtggttga tcgtgaagtt ctaaacgtcc ctgaaaatat 25260 taaaattagt tttgcttatt cacttacttc aggcatttac ttaaaaacag gcaaaaatga 25320 aaaagggcaa tattttcagc cattcaacac tgtcagtggt gggggatggt tcagaaaaac 25380 cctttagctg acccatttaa agtagttatg ttagatactg aaggtaagct ctgtgtagta 25440 acagtattta atgcaaaaaa ctgtactgat aaacatcaag ctactatgaa gacagtagca 25500 attgcatcag ataattcctt ccaacaaaca ttaatttata gtggaaaatt tggaaataaa 25560 attaatgtcg ggtaccgtga attctcaagt aatcaagcac gtcctgcatt caataatgat 25620 gttgaatatg atttaagcca atctaagcaa ataggttata aaggtgcttt attggaagta 25680 attgatgcca ctaatcaaga tattacttac aaagttttga agaactttaa caaggtagat 25740 taagatgagt gcaccacaat ataaaccaat gagagaaagt gaagtttgta atgctatcgg 25800 gtgggtgtta atagctcttg gctttatcgc aggtttttta tttattcttg catttggtcg 25860 aattgaagta gcttcttact atggtaaaga aacggtttgg tctggagtta tgatagcaac 25920 aggaatcgga attatattta atggattcct tgcaggctac ttatttcaaa aagtagctag 25980 tattcttcgt taccatgaga ataaataata tcttgcataa gcaccctagg atgcttttta 26040 aaattggttt aactaccctg cttggtaatt atatttaact taaaaagaac tacccactca 26100 ttgagtgggt tttttattgc ctagaggaaa gtaaaatggc acaagaatcc cgtttggtca 26160 ttgttattga ttcgcaaaat gctgaacgta atgcgcgtaa tctaggcaat gaacttgtta 26220 gcattgaacg taaaggtgaa tttgcatcta agtctatgga cagcttgtct gtagccacca 26280 gagctttagc tggacacatg gctggtttat taacagtagg ttcagccatt tcaaagatgg 26340 atacatatac tggattacaa aatcgcctta agttagtcac taacaatcaa gttgaactaa 26400 ataaagcaac ggaagacact ttccgaattg ctcaaaaaac ctattcagct tgggattctg 26460 tgttacaggt ttaccagcgt tttagtgata atgccaaaac tttaaacctc acaatggatg 26520 acacagcacg tttaactgaa acagtttcta aagctgtagc aattagtggt gcaagcgcag 26580 aagctgctga tgcagcttta gttcagttcg ggcaggcctt ggctagtgga acgttgcgtg 26640 gagaagaact taattctgta atggagcaaa ccccagcact agcaaaggct attgctaaag 26700 gtatgggtat tactgtaggt gaattacgtt cagtagcagc tgaaggaaaa attacttcac 26760 aagaaattgt aaaagcgctt agaaatgtag aatctgatgt tgatgctctt tttgctaaaa 26820 cagatatcac aatcgggcag tctctcacac tcctaaacaa cgagatcaca aaatttgttg 26880 gcgaagcagg taagggaagt ggtgcggcac aggtattagc tggatcagtt caaactcttg 26940 caagtaattt agatttaatt gctgatgggg ctttagtagt tggtattgga tatatcactc 27000 gtgcaatttt gatgaagagc gctgctatta aagagggaat ggcttcaact ttagcgagcc 27060 gccaagcatc tgtattaaat gctcaagcag aatatgcaga agctaccgct gctttgaatg 27120 cagcaaaagc tcatctcgcg aatgtgcgag caacaaatgc agaaacccaa gctaaatttg 27180 gcgcaacagc ggcagcaact cgatacgcac aagcacaggc agcagtaact gctgctacaa 27240 atgcacaaac agcagctcaa attaagctaa atactgcaac ttcaattgca gggagactag 27300 ctaaaggggc gtttggatta attggtgggt gggctggagt tgcaacatta ggagtaatgg 27360 gattagcggc agcctattct tattttaata ataaggcaga ggaggcaaag caaaagcttg 27420 ctgaacaagc taaagttgct gagaaagctg atgaggagtt aaaaaaatta actggcaatg 27480 ataaggctaa agcagttaat gatttaacta ctgcttttaa tgcacaaaat aaagcattag 27540 agaaatcatc gcgtgctgta gggtctgcat taattgatat cgagaactat gcacgaggaa 27600 atagggaggt tgaaaaaatt tcccaagaag cgagaactgg aactatcagc tatacagaag 27660 ccattgaacg tctaaataaa attaagttgc ctacagatct atatgaaaat ctgaaaaaac 27720 aggctgcgca gtatgatgac aatgcatcta aagcaagttt atcagctgag aaacttaaat 27780 tattaagagt tgaagtgaaa cttggaggta atgaagcaca aaatgcggca attcagcatc 27840 aaaaacaagc ggatgcttta ggaaatactg ctactgaagc agaaaaggca actaaggctt 27900 tgcaagatta tcaagccaag caaaaagata gcgttattga ttcaatctat aaatcaggtt 27960 ggcttgataa aggttacact gttgctcaag ctaatgccat tttagaactg caaaaagcta 28020 aaggaatgag tgcaattttg tctaaagatg aaattgatag cgcacttaga aatctcaaga 28080 tcatcgaaga acaacaggag cgagaagata aattaactga agctaaaaga aagcagacgc 28140 aggaaattga aaaacaagca aaacttacta aacgcttggt cggtatttcc ggtcaatccg 28200 gtattggtac tggtccacat cttgacgtcc gatatggtgg ctcattgtca ggtcagaaag 28260 tttctaatga acatctggct cgattacagg cgggaggaaa acctttaact tcctacaaga 28320 tcagttctaa ttatggtcca cgaaaagccc caactaaagg ggcttcttca tttcataaag 28380 gtattgattt ttcaatgcct gaaggaacac caatcacgac caatgttgct gtgaaagata 28440 tcaagacatg gtatgacagc aagggaggtg gttatgtcag tgaagtgatc tttgaggatg 28500 gagtgtctct taagcttcta catcaatctc ccaagatgca gagcaaggtg aaaggtggtg 28560 caagtaaagg aagtgataaa gcagctggtg atattcaatc tcaacttgaa cgtcaacagg 28620 atttgcaacg gtcacttgaa aatgaggtgg ctagtgaagt cggacggatt aacaataata 28680 gaaaggcaag actggaggat gttgataaag caaactttag cccggaacgt actgcagaaa 28740 taaaggcgga aataaatcgt cgtgcagata atgatattgc tatagccaaa caagccctta 28800 gaacgaaatt ggaagactat aaggagttcc agaaaaccga ggaacagtta ctagaagagt 28860 cctttaaccg taaaaagttc aatgcagctc atgaccttga attaagtaag tttgagcaga 28920 agcaagctgt tgaattgctg gaacagcaaa aacagcaaga gttagggtta ttaaaactag 28980 ctcaggaaca gcggttgttt caagcccgtt tatctctgct ttctgaaacg caagccatgc 29040 aggaacgtta cagactcgaa cgggaggaaa ttcttaagaa taccaagctt tctatagaag 29100 agcggcaaaa gctaatcgca ttatctaaag ccaatcagga taaagagaca cgcgataaag 29160 tgaataatgc tgttcaaaac tggggtggta tccaagcgga tatgaatggt accggagaat 29220 ttttcagaca ggatcaggaa cgatttagcc gtttaaatgc tgcaaatgat ttagcagata 29280 gtcaatttgc tgctactgat cttgatgaaa aaaatggttt agatgttcta aatgcacaca 29340 tggaagcagg actcatcaag caacaggact tcgaaaaccg gaaaacagct atcattcaag 29400 ctgctcagga ccaacgcaat cagatcgctg ccgaatatgc tcagaatgct caggatattg 29460 aagataagta tcaccaagat cgattgaatg ctcaaattgc tcttggtggc caaatgatgg 29520 gttcactcac atcgatgttt ggttcaatgt ttggcgagca atcaaaagca tacaagatca 29580 tgtttgctgc tgataaagct tatgccattg cagctgcagg tatttctatt cagcaaagta 29640 ttgcaaaggc ggctagtgtt ggttttccag caaatatccc attaattgca agtgctattg 29700 cacaaggtgc aagcatcatt gcaaacatcc gggcaattaa agatcaaggc tttgctgacg 29760 gtggttacac tggatcaggt gggaaatatc agcctgctgg tattgtccat aaaggagagg 29820 tggtctggtc ccaagaagat attaaacgct ggggcggtgt tggcttagtc gagaaaatgc 29880 gtaagagtgc aaaccctgaa gcttttctca ataacaatgc cttggcagat agtgtcatgc 29940 gccgtgcaat gatgagttct aatgccttta tagaaagcca aaagcaggct gacatcttta 30000 atcaaccagt tcaagatact cagattatct ataagggtaa tagagacaca cctaagttag 30060 cttcttcggc aaattctgac ctgttccatg atggcaaggt ctacttctca tccaatggta 30120 tagttcagga tcgctcaaat ctggatgatg ttcaggactt tactttagga cgtacttcac 30180 gccctcaagc tgagattatg ccttcaattg agcaatcctc tccaactatc aatttcaaga 30240 ttgaagtcgt gaatcaagtc agtggtgcaa ctgttgaagc tgaacaattg gatgagaaaa 30300 ctgtccggat cattgttaca gatgaactgg ataagcagct tccaagaaag gtaccgaaac 30360 ttgtaagtga ccaaatcgca aatccaaact caaccattag tcggtctttg actgagaata 30420 cgacagcaag acggaatcgt taatttaaaa gctaccttta gaggtagctt ttttaaataa 30480 attaggacaa aatttcaaaa aattggtgaa tattcttatg cttcctccag ttcctaaaac 30540 taagtcatca gaagtaaccg atattattaa ctctgctgtt cttactggat cgataagtga 30600 atttcagtat tttagatgca aacggttgct taatgatatt aaagaaactg agccactaga 30660 ttggttttta ttaagcaaca gtattattga aatgtatttt gataatccta ttcttgcgca 30720 tcaatacgct cgagaagtac tgaaaattag caatagtgta tcaattttat cgaatcttta 30780 ttttgttttt cttagctcag tagatttttc tagtgctaat gaaaatattg ataaaattat 30840 aagtttgtgt agtaaacaaa atttaccctt agaaagtttt attcctattg acttcaaacc 30900 tataacttat tttctagatg gaattttaaa tgatgattta aattattata aaagatttaa 30960 aaaggaagac tttaatgaat ttattcagtt ttttgaaatt aaaaataaac tagaaattga 31020 ttctagagtc ttgaaacata tcggttcaat tctttttaaa tgttttaact caaggaatgt 31080 tcggtgtcga aaatatgaat atagttttat tgatgatgaa tttttaatat tgctttatgt 31140 cgatagaagt tttgatgaga ttgacgctat gaattcagaa atatttagta aatgctatga 31200 tgagggttta attgatgaac tgaataaact ttcatatttt attattcctt atgaagtggg 31260 cgtggattga aaaatggcta ctacagatac actaaattac tgttatgagc tgttaggtaa 31320 ttctacaaaa tatgatgaat gtcacaaaag gaatattata gggcgtgctt attaccatgc 31380 tttttatgaa gtccgacatc atttagaaca acgactatta tggccagtaa caaagacaaa 31440 atgtggagct catgaaaaag tctatagcag acttagtggg taccctgcgg gttcaacgtc 31500 tgaaatgatt cagaaaagag ctgcggaaat caaaaatcga atacaaaaat taaagaggtt 31560 tagaacaaca gctgactatc atcttcatct aacgatttca aatcaattaa taaactatat 31620 tttacatgaa tctagtcaga tatctgaaga aatatcaaga ctttagttgt taaagatact 31680 tttataccga cccattatga ggtcggtttt ttattacctg aaggaaagtt atgtacaagt 31740 taaagctaaa tcctcagacc agcggctatg gcgtaacacc aggtgatgat gtgaaacgtc 31800 agcagatgga tggcggacgt ggtcgctatt acatcgatgt aaaacgtaat agtcatattg 31860 tcgatgtgaa ctggaattta agtaaaaccg atttcaataa aatgatggct ttctggcgga 31920 tctatcagaa taagccagct tcattctatg cggatttggt catagaccaa ggaacacgtc 31980 agcaatacca atgcaatttc attccgaact cgttcaagac caatgaagtg aatggcaacc 32040 tttaccgggt aaatgcacag ctcgaagttg ttcaaaacca gcctaacctt acggccgata 32100 tagctttgat taaggattgg gaggtctaat ggataacgaa tacgccaagt tctttttcaa 32160 tcgtaaagtc gatgtttatc aactggaatg tattgagctt tctcatcctt cctttatgaa 32220 catataccga atagttcgta atgatgatcg tggggtgtat gtacaacata aggaaggatc 32280 cggtcaggtc tattatgaat ttttgccagc atctattcaa agatccggaa tgctgggtga 32340 tctggaccag acattaaccg tttctatctc tggtctaggt gatgtgatgc cagatgagtt 32400 tgaacgggta atcgaagggc aatatccaga tgtaaagcca accgtaaatt accggattta 32460 cagttcagac aatctgaact ctccaatgtt ttatttactt ggactgcaac tctccagtgt 32520 tgccatgaac cataaagctg tgacgttcaa agctgaatct ccacgattaa ataccgctaa 32580 aactggagat atctttgcac tagaccgctt tactggtctc aagggggcta tatgaaaagt 32640 catgatcatt tgcttgatag acaatatgac gaggaaaact acaactgtgt tcattttgct 32700 catgaagctg cattggatct atatggaata gaccgggtgg aagcacttga attttttatg 32760 aagcctatta aagaaaaggt atttctacca tcaaggttaa aacttttaaa tccactgccc 32820 atgcccaagg aaggctgcat agtcgccttt cactcgagat accgaaacaa gcccccacat 32880 gtggggcttt ttcgtttggg gcgtattttg catttgcagg aatcaggcgt ttcatggatg 32940 ccaattcaag tcgttcaagc atttggattt aatcgtgtga gtttctatga ttaagattat 33000 ttataaacaa gaccctttat ccgaagacaa aacaattgaa cacgccgaaa ctttgggtca 33060 atggcttact tcaaaatatg attatatgcc tgaacatgtc cgtattttcc atacaacaag 33120 taatatggat catgccgaaa tttcatttgc gaatgaagtc acaccgaaga atgcatatga 33180 attaaagcag ctcgatttct tgccaggcac tttcattgta attgagaatc ccaagggtat 33240 agaccccata actctagctt ggatagcggt tgcttctata gttatgggtg tggctgttgc 33300 attattaatg cctgtgccct caattaccca aaccaaccag aataacaatc aatcctcgtc 33360 tgcaaataac gaattatcaa accgtgaaaa taaaactcgc gtaaatggtc gtatcgcaga 33420 tatttatggt gccgctcacg atacccctga tctgattact gtgccttaca aggtatatga 33480 aaacaatgtc gaagtagagc atgttgttgg ttgtattggt cgtggtcact ataaaattaa 33540 cggtgcatat gatggtgaaa ccaacattgt tgatattgcc ggcgcatcgg tagaagtctt 33600 tcgaccaggt gtagatattg tttcaggtga gccatatttt tcgcttggta ccgaaattac 33660 cacgccgcca ctaacggttc agcatcaaac ttctgttaat ggccaagttc tccgtccagc 33720 agatacacag tctttagaag gtacgaacta ccttcatttt gcatatccaa acgagatcct 33780 tcgggcatct gcaaacaata cggatttaac cactaagttt gtaagtaatg accgcgtaga 33840 aatcaccaat gcctcattca cgtttaacgg ccagacttat gatttaaacg gcacttacag 33900 cgttctatcg gtagcagatg atcgaatgac gttatcaaat ccggcggccg ttaatgctaa 33960 ctggttaaag cttaaagagt taaataacca acaaactgca gctttgtcac caaagatcag 34020 ttcaataggt gaaaaatgga ttggtccatt cattctggac aatgttgaac gtagccgggt 34080 gctgtgtaat tttgtggcca caaatggact ttataccgtt tcttcaggtg ggaatcaggc 34140 cgctgttaac gtcacgattg aagttgaagt aacaccggta aatgaatctg gtgcagcgat 34200 tggtaatccg atgctgaagc agatcatttt gaaaggttcg gcaaagtcgc gtcaaaccgt 34260 tggcgcaacg ctggatatgg tgacatttca aggtcgctgt agtgtccgtg cacgtcgttt 34320 aacaccaaca ccggcggtta caacggtagt agatgaagta aagtggcagg cgctttatgg 34380 tgcttatcct ttgcaaagca cagtgtatga acatgaaacg gtttttcgtg cgcgcactta 34440 tgcaaccact ggagctttat ctgttaagtc ccgcaagatc aattttgatc ttcagcggat 34500 gttaccgact tttaaaaacg gcgcaatgac gacagagcta tttccaacat caagctttgc 34560 tgatgcattg gtttcaatgg cactggatga caagataggc cgccgtacga tcgacgaaat 34620 agatctggaa aatatctatc ggacttataa cgatgtagtt gattattttg gtacaccact 34680 tgcggctgag ttctgtacta cgattgatga tacaaacctg tcttttgaag agctggtcac 34740 caatctttgt gatgccgtgt tttgtactgc atatcgtcaa aataataagc tcaagcttta 34800 ttttgaacgt ccaactgata actcggtaat gctatttaac ttcaggaata ttattcctga 34860 tagttacaag catgatctta cctttggcgt gatggatgac tacgatggac tgatctatga 34920 atacacggat ccggccgacg atagtcgtat caatatctat ctaccggata aaggggccaa 34980 gaaccccaaa gaggtgaaat ctgtaggtgt gcgtaacaag tggcaagctc attttaatgc 35040 gtaccggctt tggaacaagc ttcgcttcca gcgcaaatcc attacctttg atgcggcacc 35100 tgagtcagaa ttactggttt tacgtgaccg gatcgctgta gctgattatc gcaatggtat 35160 tcatcaaagc ggtgaggtgg tacagcaaga aggtttaatt ctcaccctaa gccatgatgt 35220 cgatttcatt gcaggcaaga gttatgtgat ttatttgcaa atgggggatg gtaccgtgga 35280 cctgattccc gttacgccgg gttcagccaa gaacaaagta gttttagggc gtttaccgaa 35340 cggggcctta aagcttagtc ccgatgactt tgtgaatact atctacaccg tagttaatga 35400 cgataccaaa ggctcactgc cttatctggt tgcaaaaaga gaaccggctg accagttctc 35460 taataccatt actgcaatta attacgatga acgttattac ctcaatgaca aggactttat 35520 tgatgtgccg gttgatgatt caccgattta cattcgatat gaccagctgg atattaatct 35580 ggcacgttta tatcagatgc aaagagggga tttgccaacg actggagaaa tcagttttgt 35640 agttgaagca ggtgcactag tttcaagttc aagttcttat cgaccggaaa ccagatttgt 35700 ctataaattc gactataact ctagtcctgc aaaacgagag tatatcgttc cagctgcatc 35760 agaattacct gctattgata ctggtgagtt cccacctgat ctcgtggtaa atttgactat 35820 taaaggtgct gttgttggac gtggtggaga tggcgggttg ccacatttgg catttggtgc 35880 atggtctacc gatccggatt ataactttac taaaacccgc cgtgacggtt ttcagggagc 35940 acccggttta ttaaaccggc acagtaaact aaacctgatt attgatggtg gaactctggc 36000 tcgaggcggc tcaggtggtg gagcaacacc aagcggtatt tatacaggat tatcgtatgg 36060 agttcagggt attcccggtg gagctggagc accttttggt cgggttatga ccggacaacc 36120 tattactaac gattcacaag actggcgttg gtacttaaat ggtgacttta tggttgtcaa 36180 agtaaccgat gccgaagctt cggtacccgg taaaggttac cgaacccaaa atgatcgata 36240 tggatctcca ttgtctggtg atggtggagg ttggggccag cgcggtacca agtccaccaa 36300 tgatggaaca tggaactggc aataccatgg cacaactgaa ggccagccgg ggccgggtgg 36360 acctgcaatt gttggggtgg caccacttac aactcaattg atcaatggag ggaaaattct 36420 acaaacactt taaatcttaa aagaactttg agcacccaat tcgggtgctt ttttattgcc 36480 taaattttct ggagatataa atggaaccag tttcaacaag cggtttaaca gcaattttaa 36540 aattttatgg tgcagcaatt atggtgacgt tagcggttgc tttagttgca gcagttgtat 36600 tgatgacacg tatgccacga tcacctcaag aatgggctgt aggacttatt tgtacggttg 36660 tatcaagttt ggctggcggc tcattcatta ttgtgaagtg ggggcttcat gaatgggtta 36720 ctgatgtatg gggaatgatt gcacttggtg ggttcttctt tgtttgtggt ttacctggtt 36780 gggctttagt ccgttggatc tttaatttca tagataaaca ggaagggaaa acgattgttg 36840 aagtgattaa agagtttaag aaagccagaa aagacattga aaacagctaa tgccgccttc 36900 gggcggtctt gtttagaagt acacgtataa gagagaaatt acctgttgac actgcaagcc 36960 gctgactact acgaaaacct attgacgacc aatattatga aacgaccacc ttcgggtggt 37020 tttcctttat gtgacattta gtaaccagtt tgttaaagtt attatattta taacaattgg 37080 tgaaattcat gaaaaagata attttaggga gcatgttagt ggctgttttt tccacatcat 37140 tttcacatgc tttagctccc aaaaatggag atgagccaac ttattgtgag cagattgttt 37200 cggtccatgg tttattaact agagcacaat ttgaatgtgg atatagtgaa tataacaatg 37260 agttaatctc agattcagcc aagtgttttc agcatgaact tggcgaagaa tatggaaaaa 37320 aagtccttat atttggcatg aaagaatttg accgaaatgt aaagaaagac gggaagaata 37380 agatttgtaa tagtttatta aaagaatttc cagagtatgt aaggaagtaa ctgatgaaaa 37440 agctactacc aattgcattt ttactcacag catcatttgt aactcactca gccgatacta 37500 atgataaaca ctgtagagat gtgaataaac ttgctgaaaa tgtcatgctc tttaggcagg 37560 aaggggtttc tgtggttaga caaatggaga tgatagagag tatcaaacca agcagggatt 37620 tcaaaaggtt aatggagatg atggtcgagg aagcctataa agaaccaaag tttggatcag 37680 aagagtataa ggcggaagca ataactgaat ttgcaaacaa ttggtacatt cagtgcaagc 37740 aagcaaatcg aaataaatag agcactttaa ggtgctctaa ttattgaaat tgagaaaagt 37800 ttataagtag gtttttatga gaaagattat tttattgggt cttatttgcc ttcctgtatt 37860 cgcatatgca aatagttgcg aggtggcaaa aagtaaaatt aatataagcg gtttagcatt 37920 gggtaaatcc attttatcac tgaaagcaga acatcctaaa aatttgagca tagatcatga 37980 gactaataag gcaaatatta actatgttca ctctaatgaa tttgaggatg cttttagtgg 38040 aacaccagct accaatgcgg gattcatttc ttttgatggg aatacaaagt taattaatgc 38100 gtttagcgtt agttttggtc acttagataa ttttagtgct aataattata aaaatgggtt 38160 agtggcgttg tattcactac caaaaacagg gtggatagaa tcaaagagta atggggttaa 38220 agtttttaaa tatgaatgta cggattattc tttagagatt aattacaatc cagaaagaag 38280 tagttttatg atttttaagg aaatttagtt ttatgttctt aagcacccta gggtgctttt 38340 ttaatgtctg atttttctga aacagtaatg gtgtaacctt tcatacggct agctaactcc 38400 atcattagag tttcggtagg gatcagttct acagtttttt cataattttg attttcaaag 38460 cttttttcaa gacgggcaac aatgtcggca ttcattgatc gactgtttaa ctttgctgat 38520 tcaagtattt tttcttttag ttcttgcgtc atacgcattt tgtattcaac gtctgagctt 38580 ctagccatgg tcctatactc gaataaattt tatttataat aatatcccca atggggattg 38640 acaagaagtt tttaaagtct taaattgtaa aagtccccat tggggatgta aaaagccccc 38700 aactttctga cggcaagggg cttttatcaa caaccatagg aaaggatatt gatatgtcta 38760 gtttagcatt aagttttaat gaagtgaaat tcaatcccgt gccacggcaa gatggccaga 38820 ttggctttc ttcaggtgaa ttggcacaag cattaggata taaacaagag aacgcggtca 38880 gtaaaatttt taatcgtaat tctgatgaat ttacggaaaa tatgacacaa attattgata 38940 atcctcggct acccaatttg ggtatgcgga tcttctcact acgtggctgc cacctaatag 39000 caatatttgc tcgtactgct gtagcgaagc aattccgcaa gtgggtactt gatgttttag 39060 ataaagaagt tggcacacca gttgccaaaa cccacaaatc cgaacgtgaa cccctaacca 39120 atgctgtaaa tcttcttgta gctaaaacta agcatttgaa ttacagcgat gcttataaat 39180 tagttcatca gcgtttcaat gttcagcata ttgatgaaat cccatatgac atgattcctg 39240 ttgcagtgga atatgttcat catctgattg cgatgtacag tagtgcagag aagaaggctc 39300 aaggttcttt atttgataat gaaacattgg gtttggttaa ggatctggta gatgcaatta 39360 tttcccaaaa ctttgtgaca agcaaaatct atcgtgcaat acacatgctt agtaatgaac 39420 aaggtcacta cttagctgaa tatgcgttta aaaccaatat tgcagttcta aaactcactc 39480 gaacaatgga tttaagagga cctcttaata gagaaatcat tagtgatgat ttaaaaacca 39540 taagctacac aacaggtaat caacattatg gcgaccgttg gtttcaccca ctgatggagt 39600 caagtcgatt gatgggagta cttgaaattt caggtagtct gattcgtcac taataaaatc 39660 aacttaacaa aacccactca tcgagtgggt tttttaatac ccaaaacaaa accccagtag 39720 cgctaactac cggggttttt cattccaccc accgacgaaa gtaagaggaa agtaaatcta 39780 tatggagcat tttaaaccaa tagtggagct tataaaagtg tctattgaaa agtatggctt 39840 atggcaaaca atagttgcat ttattctttt gttttccgtg ccaatcttaa tgtggaagtt 39900 ggatgtaatt attgcttcta taaaagcatg aaccaacttg aaaaaactgc gccaccttcg 39960 ggtggctttt ttacgtctaa aggaaagtga aatgaacatc gaacaatatc ttgatgagtt 40020 gatcaaacga gaaggcgggt acgtaaataa cccagcagac cgtggtggtg caactaagta 40080 tggaattact gaagcagttg ctcgagcaaa tggattcaaa ggtaatatgc gagatttacc 40140 tctggatgtg gccaaagcaa tttaccgcaa aaactattgg acagctccgc gatttgacca 40200 agtaaataca atcagctcag cagtggccga agagcttcta gacactggtg tgaattgcgg 40260 taccggcttt gcaaaacctc ttttacaacg agctttgaat ctcctaaata acaatggtaa 40320 agcagggtgg ccagatttat cagtagatgg gatatatggt ccggcaactc ttaatgcact 40380 caaaacttat ttggtcaaac gcgggaaaga aggagaaaaa gttttagttc gagttctgaa 40440 tattatgcaa ggtcagcgtt acattgaaat ctgtgagcgc aatccaagcc aagaacaatt 40500 tttctatggc tggattgcta atcgagtatc aatgtgaagt acctaatttt actgtgcatt 40560 ctactcaaga ctgcacagtt acttcgacgt atagtgaggt agttgtaaaa gtttataggt 40620 aagttatagg attgattggt aataatcttt aaattttagg gggggattgt tcagatgtag 40680 tgtattctgt aaaataaaac ttaattatat tttgctttca atacaatgaa cgatcaagtt 40740 ttccaattac aaattgttat aaatggaggt ttaaccccca ttcaatctaa gccagaaaca 40800 cttgataaat tagtaaaaga atttgctata aatcatttgt tgtttccaaa agaaataact 40860 gaacaattaa ttgagattaa ttctcaggat ggttctcaaa caaaaaagat aactaaattt 40920 attgatttgg ttagtagcaa tcaaaaatgt acctaccaaa ttagaaatga ttcacttgta 40980 tttttaaatt cctttgagaa aatagaggaa ttagaaagta tatttgaaaa gttttttaaa 41040 tcgttttcgg atctgacccc atatataaac tataaacaat caaaaagatt ggggctagtt 41100 cttattaggg aggattataa tgaggttaca ttacgcgagt attgtacttc agaagaatta 41160 gatcgaaatg ttattgagaa tagatcgaga aaagttactc gttttgctat ggcggaacta 41220 aatgaaatgg taaatttatc tgtttcaaaa gattatgtaa ctcatgaatc aggagtttct 41280 cgaaatactc tagcaagtgt ttatgatgtt aatacgttat ctactaagga tgtttttaga 41340 tttacaagta aggatgtagt aaagtttata aatgcttcaa agaaatttat tttagaatca 41400 atgtaagatt ttttatatga actattcaaa taatttatct aataatacta tgtatgaaag 41460 tggtaatcat aaaaagataa tcactaagta cgagattgaa acaataaaaa cggtttggca 41520 aaccgataag atacagtctt ttttaaaaga ttacttaaat acagacgtag ttagtattac 41580 tgaccccact gttatagaac aaaagatagg ggaagagagc ttaaagcaaa ttaaaagaga 41640 atttgatatt tttaagaata aatttgataa ttttctgaga tatgaagatg tgccagtcga 41700 ttatgtttca cctattgaaa atgaattaat caacttttat aaacatagta aagttgaagt 41760 tcaagaacaa attagccaat ggattattga ttcttttgat aacacaaagg ttcttcttaa 41820 tattttaaaa attttaggta atattgctcc tgattttatt gatcatcaat ttttaactaa 41880 ttttcttatc gttcttaatc ataaagatac tgaaatcaaa gaatatgcat taagaattca 41940 agagaaatta atgcttccat catataataa tgtactgaag cactctaagt taactccaaa 42000 atggattgat gactatagaa aagaattggt tgaattgtat gaagaagata ataaaggtag 42060 ttaatttaat atgactattt ttgtaagaaa gataagtaaa gcaaaatggc cttctgaaga 42120 ggaaattgca gaaaaagcac tggattcaga aattatacct tttgtcagag ccgatgcctt 42180 aactacttgt ttaaaaactt ctcaaaatac tttatctgtt tgggcagaaa gattaaaaga 42240 aataaaggcg gtttaaccgc cttaaactgt tgcaagctgt gctgtattaa gcacagtctt 42300 gctttgctca tatttcaaaa cgtctttctt tttatatgaa acacgtctcc caattttcga 42360 gaaaggcagt gatgattgat cacaacgcat tctagctaat gtccaaggcg agcaatctaa 42420 ataaagtgct acaacctctt gaggaaactt ctgttcttca ttagccatta tgaagcgatc 42480 caaatattct tgttgctctg catcagatag atttctcaga tcttttaaca tttactcctc 42540 cttactttcc gctttaactt ctcgatcaag acgctcaatc tccgcaatta ataaagcggc 42600 tgcacgaacc aaatcttgtc ttgggctttt tggtttccag aaagtatcag caaaaggcca 42660 ccatcctgca gcatcctcat ccatatacac ttcggggccg aaatctttgc tgttataaac 42720 ccaacctcta cttactacat gatcaacgta acaagatgca gctcgtggca attcatttgc 42780 ttcatagaga ttatcaaatt caggtaaata cccttctttt ataatttggc gttctctttc 42840 attaagaaca tctttgacag cttgactgct atataattca ctcatccctc agctcccgat 42900 tcgcttgcca cttcaaccat caaagaataa ataggcgtgt aatgatcacg agtcccatcg 42960 ccccaatgga atccactatt ttctaaacag ctaacaacgc tgtctggaat ctctttcggt 43020 accaaacagt aaccctctgg caccgcctga gctttggctt tttctagctc tgcatcacga 43080 tgctttgcac atctaagcca agcatcccaa cgtctattca tgttgcttat ttctttctga 43140 gcaatttcag aaggattgtt tgatctagtc ataaacagtt catgctcatt actaaaaata 43200 atgtctcttc ttcctttgta atattggaag gtattcagaa aagcctctct ttccttattc 43260 aaatcaaaca tcattaggcc ctcaaatatt cttctttagt ccactcaaca aactctttat 43320 aaagctgctg cgcgggttta tttaaccggt tgtgatagtc gatcgttatg cggcgccaag 43380 cgactggtac cgcataatgt ttggttagaa acattgcttg atccatgcct tgccggacta 43440 ttacgtagcc cagcaattgc aagtagtaca taaaaccaag catgtgtttt tggctcactt 43500 tcttgtactg atctttcatg ttagaaaccg tccactaata aataatcagg gggagattct 43560 tgttgagtag gtgtaggatt ctctaattca tagcggcgtt ttctcacata ccccattagc 43620 ttcggttgaa tctgcggatc tcgtgcagcc acgtctattt ccaaagcatc tagcgttgta 43680 aggtctggtg cagtttggat ttgaaccatt aaagagggtg gctcattagc agatgccttt 43740 tctttttcta gctcttcaag acgtttgtga gtggcgagaa ggataggctt catttgttcg 43800 tcatcccatg tgcgggtata acgataaacc gcatttactt ctgcaggtgt ttttgactct 43860 tttacacgct gtagaagagc atctaatgcc ttctgatatt caggatctac tttaggctcg 43920 ttagtttctg gaactaacag atcctcagat gtggtgacat ttgtttgttc ggtaataaca 43980 attgttggtt gagtttctgc agaaataacg tcgctaggct tttctgcttt tgattttttg 44040 cctcgctgtt ttttgggctc ttcaccaagg cgaacaacgc taagatcatt attaatttca 44100 aaaccgagtg cttttgaaaa tgcttttaat tgaagcttgg cattttctgc atcacgttga 44160 acaaatcctc ttttaatagc ttcaatgagt gcagcagttt caaatcctat gatataaatt 44220 gaagggagat atgtattgat aacaaaaaca tcctgacccg ctttatactc atcaatagtt 44280 aatggctttg tgaatgtaat gccagccagc tcaatagttt cgattttgat gcagaattca 44340 aaacccggtt taccaaaaac agaagcgggg aattgatcta agtcagaaaa gtccaacatt 44400 tctccaatag gacgacatag aacagtttta cctttttgaa gtgctgcaaa tgcttcttga 44460 gcagttaaaa tatttttcat gctgtcatcc ccgttttcgc taaggtttca atttcttgtt 44520 taactgcctt aagttttgcc gcttcaattt ggataagggc atcgatacct aagtgctcac 44580 aaactgtttt tacatcgagg ccacgttcag caataaagtt ttgaagttca tctctttgtt 44640 gatctgagat accgttaaat tcaggtggac taatccaagt gccacgttgt ttatcaaacg 44700 tgcaattcaa tgctttagct ctcattaaca ttgcttgtcg catgttctgg taatacatgt 44760 gttctttatc aagcgactca gttaattgat taaggtcacc tgcatgctct gcttcctcac 44820 agctttgttt ccagttttct agctcttctt gggctttagc tgctgcaagt tgtgcaggcg 44880 ttaaggtgtt aatgtgatct ttagcttgag taatcaggtc agccaagaaa gtagggtgtg 44940 ctttaagatc aggtacccat acttcaccgg tttcaccgcc taaagcacct gagtttttcg 45000 catgatgtgt aggcgaaggt ttgaaattaa taacgcgggc atttttacct tcaccagtag 45060 taacagttgt tagataaccc atgacatctg cgatacggta aagctcgtta cggtttttac 45120 cacctagatc tgggcggtaa ataatttgat caccgttttg atcttctgat gcgtgtgcaa 45180 tgaaaacaac atctttacct aaactgatca aagtattgat gtattgcttg aacgtttggt 45240 tcgctaaacc ttgagccttt aactttaaag agccatcttt ttgacggtta ttagcagtta 45300 gcaatagatg ggttttaatg cattcaagca ttgcacccac ggtatcaatg actacggttt 45360 tatatggtgc taagtcctgc ggagtaaggt ttgcaacatc actccattgt tgaacctgta 45420 caaccgcacc acgacgtaat tcaccagtac ggtgagcacc acggtcaaag tcaaaagaaa 45480 ttgctttttc cgcagtaaag cccatcgatg atttacctaa acccggatca gcgtataggt 45540 acacaataat tgcttgaacc aataaagttt ggtcagcagt aataatcggt aacgccattt 45600 ttcttatcct tatcttgagc cagtgaagcc gcgcttagtt ttataagcct tgcggtcata 45660 agtagggatg tttgtttcac gcagttttat tgcgagctgc tttctgcgtt ggaaatcaat 45720 ttcttgtgtg agttcattcc aaacttttgg atagtcagtt tggaacctga acacatttaa 45780 aggcgtctta aatccgtctt taactttgta aagaactgag ccattagcat tagatgcgta 45840 cacttgccag ccgatgcgga cagagtagag gcccttatca tcacggccta aaaatgactt 45900 gtagccgtca gggtgttttt tgaaattagt catctttaag cctgcaccaa cttgttacgt 45960 tcgatgaagc cttttagaag gccattgatg tttcggatgt cttcaaattc ggtgaaatcg 46020 ttatatgact taccattaac atcagtgatt tcatttactg tgagttgtgt aatatcaaca 46080 gcagtgaatt cagaacccgg aacgccgtag ctgtctggat gagcttcaaa atcaaagcta 46140 acgtttaaac ggaagctatc taatttaatt acagcaacgc cagaatgttt acctgtgatt 46200 ttcgcggtta acacaccgta agtacttggt tgagttttag gtgtaaaaag agtaggtgct 46260 tcttttgttt ggaaagctgg ctgcaattgg caagcaacta aagaacctcc agagattgca 46320 agagcagcca tgctgacaaa tgcaaatgag ttgaaagggg tagcttttac gttcataatt 46380 gatctcgcag tttgcaaaag cacatcagat ttagcggtcg gtgtgctttt ttgttgtcta 46440 cgagacaaat atagttttat ctattattag tgtcaatagg tataactata aaatgatagg 46500 tgtaaatttc taaattgata ggtaaatcta taagtaaaaa gcccacctta taggtgggca 46560 aatgtataaa attgtttcta taggtgaatt tattaaccag ctcgccaaaa ttgacgaccc 46620 attaccttaa aattttttcc attctcttca gtaacttttc gatctctaaa cttttcattg 46680 agactatgaa gaattagaga cccatcagcc tctttaaaaa tttgcttcaa cattccttca 46740 ccagcgaagt aaacagcata tatttcaccg tcaataactt cagtttgaga aatatcaata 46800 ccaactaagt caccatcttt gatgtaatcc atcatgctat cacctttagc tttgatgata 46860 cgcatgcaat cctcagagac acgtttttct ttaaaaaatg atggtggaaa agggattgct 46920 ccattgattg catcaaaatg aaattcaata gattcacctg gtccacatga aaaattagct 46980 tccactacgt caatccaaat gtaattgtct attcctttat gaaccacttt aggttttaca 47040 aaatcatctt cggaaaaacc atcttcagtt tttccagtag ttagccaata cgcatctaca 47100 cctaagtatt ttgcaatcag tggaaggaaa gcagacttca agctcttacc actttctaag 47160 gcttgatatg tgggttgttt aataccaact gcttcagcta cttcatactg acttttacca 47220 gctttagtac gagcttcttt gagtcgagtt gccaaatcag acataacaaa aaccttaatt 47280 tgtttggaat tggattttat aggctcaact ataaaacttc aaaaaggcat acctattgac 47340 atatgatagg tataactata aattaactat caaaatagga gtttgatagt tatgagccaa 47400 aatctcatcg caatctatga aaaattagtt gagttcttag gtggccaaac aaagacagct 47460 caagtcctac ttataaaaca accctctgta aatgcatggc ttaaaggaaa aaaccaaatg 47520 tccgaaaaga ttgcattacg cgctgagcgt gtaactaaag gtcgttttaa agcttatgag 47580 ctttgtccat cactttcaga attcgaaaca ttaaccgctt aggaccttac ccatgagcaa 47640 agtatcaaat gaattgcctg caagcgctag caataacgaa tcgctcatat tgcaagcact 47700 taacgctagc aaccaaagac aagtagcaga gaaggtggga atagatgcaa gcaccttgtc 47760 gagaatgaaa aatgacaaga aaaacaatgg attgacagag attgaattta ttagcttttt 47820 gttgacagcc attggtttga aggttgtgca agagagtgat gtgtattgct cacctgaaat 47880 tgcagaagca acgcgagttt atttagcaca tgcattcact tcacctgaat acatgcggat 47940 tttattcaaa taaaaaacca ctacctgcgg gaacaggagt ggttaggcat tcaaatgagg 48000 tggatcaaat gaacacaaac aatttatcag aacaaccaat cgaactcaac tcaccagatt 48060 ttttaatagg tgacgttgta gtgcttacta aagagtgccg tactttcaaa tcaaatgatt 48120 tgtttgaagt taaaaataaa actttgacta gtttatggac tatcaaatcg gagaatcatt 48180 tgattctggt ttcatcaaaa gaaatccgta cagcaacagt agcagagctc aacgctaaac 48240 gccgcctaac aaaagctgag caagcattag cggaggtgtc atgaatagtc aatttaagta 48300 taaacctgag tacaaacaga ctcaggaaat tcagtccttc tttgatccag cgttagtgat 48360 tctcaatgag ctacatgatc gtaaccgtaa aaatctaaga gccaaaggtt atgacgaaaa 48420 taatgctgca ataacgcgtg aagaattttc acaaactatg gcacagcgtt ttcgcattaa 48480 tcagtggtta gcagggcaga tcgttaatag tttggctaat gctgacttgg ttcaaaaatt 48540 tggtgggtat gtaaagccta aggtcggtgt acatgagtaa ttttgtgcct aattcctttc 48600 aagtgcctaa tgcatttgtt gacgaggttt taaataaaat ctctgatgct gcatgcaaaa 48660 tttatttagt tatttgccgt aaaactcgtg gctggaataa ggagatggat tccatctctt 48720 taactcaatt tgaagagatt acagggaaga gtagaccgac agttgttaaa tgccttaatg 48780 aattaattaa agttggttta gtcgtggaac aaccaagcac aattcatgga aatacattca 48840 aattaggtaa cgatactagc gttggtttaa tgcttaaatt ccctagtaaa aattttttac 48900 tacctgaaat ttatggccaa actagtaaaa attctttacc actgctagtt aaaaatttta 48960 actacactag taaaaatttt ttaccgctac tagtaaaaat ttttaacaca caaagtatca 49020 ctatcaaaaa caactctcaa agtaataaaa aaataaataa aaaaagagag cctgtttctg 49080 aaaaacctaa atcagaaaaa ccaactgaat ttaatccacg tttagttgaa ctaccagcat 49140 gtgtagatcc agagctgtgg aacaattttg ttgatatgcg tatcagcatc aaaaaaccac 49200 tttctgaaaa tgcagtaaag ctaatcctta aaaaacttat ctcgtttggc cctttggcta 49260 accaatcact ggaaaactca attatcggaa attatcaggg tgtatttgag cctcgccaaa 49320 atcaaattca ggaaaaccca caatctcata acgttcctga agaaccgggt tatttcactc 49380 aaatgtacgc tgagagcaac cgttcaaacg tgattgacgt tacgccagtg tcacaagatt 49440 ttggaggcta ttaatcatga atgaattagc accatttgaa agttttttaa aagaactaat 49500 tgcggcttac agaactaaat acgctgttca gttcaataag aattttccag tagaggggaa 49560 aaatgccgtt ccaatgcaaa tcgttgaaca gcagcttgct aaagcattgg ttggggttac 49620 acctaaccaa cttcaaagag gcttagcgct attttacgca agtacaaata catacatgcc 49680 taacttcgct gaattccgtg ctatgtgcat gggtgatgat tggtggagcg ctgagaaggc 49740 ttgggttaag gcttgtgaat acactcagat ctctcaacac aaaaaagtga cattgccaga 49800 cggaagagag cagaaccaag aaattacaac cttgaccaaa tttgttttag accaagttta 49860 ttcactaatc caagatggtg aaatgtacaa agccaaaatg gaatttatca aaatctatga 49920 tgaatacaag gctgaagcac aactgaaggg taaaacccaa gcttggtacc aagaaccaat 49980 tttattagct cagaaaaatg agcaaaaggt gcataagcct gtttcaaatg acgaagcgca 50040 aaagcatctc caatcattga tggaacgttt aaaaatcaat ggtcgtaaac ctgtaccagt 50100 acaaaagctt aaggctaagg aaaaagagcc agaactcaaa caagagctag gtccagatcc 50160 ttttgacaat ccgcacgaat acgcagagat gtgccgccgt gaaggtatgc caattcctag 50220 aaatattctt aagttaattg atggggcgaa tatatgaata aattcgagat tttagcgtgg 50280 ggtttactca tttcattttt tacagcagct attagcggtg cggtggtttg gtggtggttg 50340 gcgcgtaaag agcttgatga gaaaggagcc agccatgaaa gcaactaaat tgattagaga 50400 taaaggactg caatacgcga aggaaatcgt agattcagca cccgataacg caactgaatg 50460 gaacgagggt tatgagttcc aatgtggtca aagtgtagaa atcagcccag cagatcgtga 50520 gaagtatttt gtagatttgg ttgagcttaa acgtctggtg gagtctttga aaatcatcaa 50580 cgatttaggt ggagttgaga agctaacgcc tgcattcatt acgacagata agcatgttgg 50640 ttacacgcat gttcgcatgg tgggaaatgg gagattgagc tttcttgatg atttttgcga 50700 cttcattcca gatggttcca tttcaattaa gcgtgtgatg actgctatcc gcgaccacga 50760 atcaatatac ggaggcggtg aatctcatgc caactagata taacacaggc gagtatagct 50820 acgatcttga atatcactat ggagatatgt cagcaagcat ggagatgctt agagcacgtt 50880 taattgaatt gttgactcct catctgtctg gccgttatgt gaaatggaga gaagcatatt 50940 tcacatggtt tacaaagtgc ggcggggatt cggggtggat gttttgtgta ggtccacacg 51000 aatttcatat tgatggggcg ttaaggcgct attactcagg ttctattgat attacctaca 51060 accagaaaga tcgatgtttc ttggtgggtg agaaaaagaa agtcaaatgt aaggcttgta 51120 aggggtttgg cttcattcga gatgatgggt gggggcatat agataaatgt gaaatgtgtg 51180 atgcagaaaa aggagccagc catgagtgag tttgagggta aatctggaaa gtgggcttgg 51240 gagattcaaa aagaacaaca agcgaaagtg gaggagctgc aaaagcgttt agatggggca 51300 ttaaaagaga ctcaatatgc tttgcagtat gttgaagaag acatgcgcgg caatcatgaa 51360 tttctacaaa tggcaatgat tcgaaccctt aaagctatag agcaagtgct caaaggtggt 51420 gcttgatgtc atcagtcagc attgctgaat accgcaagtt atttcccata aagaaaaata 51480 aaaagcgccg ttcagcaaag caagttgcca gacaaccaag tgtgggtgaa gtggttctgg 51540 caacgcattt aagagcatgc aagattggat ttgaacagga atataagttc catcctgaac 51600 gcaaatggag agcagatttt ttaataaagg gttcaaagat tttgattgag gtagaaggcg 51660 ggatctggag cggaggccgt cacacaagag gcaagggcta tttaggggat atggagaaat 51720 acaactccgc agcaatgatg ggttttacag ttttacggtt cagtacagag caagttaagt 51780 ccggtatggc attaaagcaa attgaattat taattaaggg taaataggaa ggcgattatg 51840 ttggttgaaa agtttgattt tattgagtta cttcgccttg ctattgctca aagcgaaggt 51900 aaagggaaaa ttactaagca tgttgttttg ggagaaattg ccttattgcc tgcaggtgca 51960 aaaaaatggg cagaattact gcttgaacgt attgattttg agcgcattgc agaaatcaca 52020 gaaacaaaga aaatttatga gaccaggata attaatggta aggaatcaaa aaagcgtatt 52080 ggtgaaatac cgggtaaagt tgaaataaaa aaaggggaga ttaactcagc tgattttttc 52140 cgcgttagaa acgtactagc gggtaagatc catcgtgaaa tgatcaaaaa gaactttaag 52200 ccaaataatt gtcagggcga tttatcaaat gtggccaaag gtattgctga ggttgttttg 52260 cgtgggcgat tatttacaaa ggcaatgtgt ggccattgcc agggattagg caaattggag 52320 ttatttaatg aaaagggata tccaagcgga tctaagtttt gtgataaatg cagtggtacg 52380 gggaaacgcc catatacatt gcatgaaaaa attacgatcg caaaattaaa agtgtctaag 52440 tctggttatt ctgagcgata tgaaccatat gaattaattg ctgaagcatg tatcgaaaat 52500 tgggaaaaca ccattagaac tagcctggct agatcgtttc attttgaacc tgaagaaata 52560 tcattagctt gacttaaaca gaacggttga gtataagtat ttctaaaatg ggcgctttat 52620 acatggatcg cctgaaaaac ttaataaaag ctcactaatt ttagtgggct ttttgcgtat 52680 ctagagcatt gaaaatggaa aacacctggc atgctgacca agaaaaacca gaattacggc 52740 cagatgaaaa acctttgaat tgcccatttt gtggatctga ttcaatttgt acggattctt 52800 cacattatgg aaaaccagat gaagacggct ctatagcgtg ggatgctttc acatggtgtc 52860 atgattgtgg atcaaaaggc cctagtgctt gggcgatgat cgcttgggat gaaaattttc 52920 attacgacac tgtttatgaa gaaagatcaa ttgttaatta tgctattcgc cagtggaata 52980 cacgcaaata agatttttaa tctcgtgagg ggtgttttat aagcacacct ctcttttagc 53040 cggacggatt acggcgcaaa cggccccgct acatactagt tattggcggg gctttttctt 53100 tttggagtat gtatgactga atttcaaaaa attacgcatg agattagaca gctccaagta 53160 gagttaaatc atttaggaag ctgcaataca aaagacttaa cagcagaaca gatcgctcac 53220 atagatgagc gatttttttt ggccattgaa aagcaaagta aattaattgc tcgtctcaac 53280 aataagccag agggcttctt ataaatattg gtggtgcgat ggatcctaaa aagtactata 53340 tgctcaccag gaaaaaagaa agaaaaccca agccaaagag cacaccatta ccaaaagcca 53400 agcaaaacta tttagaggcc gaagcaactc taaaagaaga attaatcgag ttggccattg 53460 gttttgaaag taagtttcag ccgatccata ccaaacactg gcgctttgat tttcatattg 53520 tgaaattgcg tttgctcatt gaaattgagg gtgggccctg gtctggtgga cgtggtggaa 53580 agctggcaaa taaagcatgg agtcttgatc gatatgatca agctgaagag atgggttata 53640 aaatagagcg ctttcatcca gattctattt tgtcgggata tgtcatcaac tggataaaaa 53700 gtgaattagc gagaattgaa gatggagcaa ataagaccat ttccaccgac tgattttatt 53760 gatcaagcag atgaagaaga agcaattaga ctaacaccag caccagatct aaaaaaatgg 53820 gttgttgcta attacttaac tattggtgga cctctttata accccgatca tgatcacata 53880 ggtgaactgc ttcacgataa tgaagaattt ttagcattcg cgtgggcctc ttctgcatat 53940 aaaagcaagc aagctatggt gttgggccag tgcgaaaaag tcatgttcaa tgtcggtggc 54000 tggcgtaaag ctcgacaaga gcaacagatg cgagactggt tcggctttgt gccaacttac 54060 ttaataactg tcgacgcttc tttttgtgag cgtgcaaatg atacagagtt ctgttacttg 54120 cttgaacatg agctttatca cattggagtg atgagagacg aggacggaga aattgtttat 54180 agcgatagtt ctggtcttcc taagcactat cttgcaggtc atgacgttga agagtttatt 54240 ggcgtagtta aacgttatgg cccaagcaaa aatgttaagc gacttattga agtcgcaaag 54300 aatccgccgt ttgtttcgaa tcttgatatt tcaaaatgct gcggaaactg tgtaatcaat 54360 tgagcctaat ggctcttttt tttgcccatt ttgttatacg tagttatacg atgaggaagt 54420 tatggcgaca ctaaaagagc ctgtgaaaat ctttatagtt cagtctcttg cttgtcgtga 54480 tacacctcaa gaagtggctg aactcgtaaa acaagagttt ggcgttgata tagatcgtgt 54540 tcaagttgca acttatgacc ctacaaaggt tgctggtaag aacttaagca aaaagtatgt 54600 cgaactattt gaaaaaacca gagatgagtt tgataaaggc ttaattgata ttccaattgc 54660 taataagtac taccgattga agcaatacca aagacaactt gagaagacta gaaacgtcaa 54720 aacagcctta aaaattcttg agcaagccgc taaagacatt ggtggtcaat ttactaatcg 54780 ccaagaaatt acaggcaaag acggcggacc agtccaaaca gttaattcag aaattccagt 54840 tccaatggaa gattacttaa aagcgcggag ggaagtctta gatgagtact gatgcggctc 54900 gggataaagc catccggatc gaggcgcaag aagatttata tttcttcaca aggtacatgt 54960 ttaaggagcg ccgtggttat aaatggatgc aaaattggca ccacttagaa atctgcgaag 55020 ctttaatgaa agtttatcgc ggagagataa agcggttaat tattaacgtt ccaccacgat 55080 attctaaaac tgaaattgct gtaattaatt tcatggcttg gtgttttggt aagaatccag 55140 actgtgagtt tattcatatc agttactcgg caatgcttgc cgcaaataat gccttccaaa 55200 tacgaactct tgtacaagaa gaggcgtata gaaaagtctt tcctgagctt acattgcgtg 55260 atgatagtaa ggctaaagac ttctggagaa cttctcaagg cggtgtctgc tatgcgacag 55320 gtacaggcgg tacgattact ggttttggtg caggaaaact tcgtaaaggc tttggtggct 55380 gcattattat tgatgaccca cataaagcac atgaagcttc atcaaaaact attcgagaag 55440 gggtaattga ttggtttcag aacacactcg aatcgcgtac taactcgcca gatacgccga 55500 tcattgtgat tatgcagcga cttcatgaag atgatttagc tggatggttg ctaggtgata 55560 gaaaagacgg cgttcctgta gctggtggta acggtgaagt gtgggagcat ctatgtcttt 55620 cagctattca ggaagacgga tcggcactat ggccagcaaa acacaatatt caaaaattga 55680 gactaatgga gcaagcggca ccgtatgtat ttgccgggca gtaccgacaa atgccatcac 55740 cgccagcagg cggttttttt aagcctgaca atattcaaat tgttgaggct ttgcctgcag 55800 atgtattgaa acaagttagg gcttgggact ttggggcaac cgaaaatgaa ggcgacttta 55860 cagtaggtgt gcgagaagct ctaggcgcag atggttttac ttacattgtc gatgttacaa 55920 gaggacagct tggtccagac aatgtgaata agcgtttaga acaaacagca aagctagatg 55980 ggaaaaaagt ttctgtgcgt ttaccacaag accccggtca agcaggcaaa tcgcaagcta 56040 gttcatttgt gaagcttctt gcgggttata gcgtgatagc taagccaatt tcaggtgaca 56100 agcttacacg tgcacaacca tttgcggccc aagttaacgt aggaaatgta cgaatgctca 56160 aaggtgaatg gaataaggat tttattgatg agcttcgtca ttttcctaat ggcacacatg 56220 acgaccaagt ggatgcagct tcagatgcgt ttaatgaatt acatgaaggt tttgaagcct 56280 tctttgctga tatgggattt gctcgatgag tgatgtaact tttcaacatg ctgaatatgt 56340 taagaacttg ccatactggc aaaaacttga tgatgtttgt gaaggtgaag atgcagttaa 56400 ggctaaaggt gaaaaatatt tgccgatgcc aaatgcacat gataaatcac ctgcaaataa 56460 aagcgcttat gaggcttatc ttacccgtgc agtcttttat gaagtaacag ggactacatt 56520 aaatagttta gttggtgcag cttttgcaac cgatccaagt tttaaatttc ctccggaact 56580 tgctcattta gaacgtaatg caaatggtgc tggtttaagt acttatcaat tggctcaaaa 56640 tggaattcgc catttattga agcattatcg ttgtgcttta tatgtagatt atcctgatgt 56700 gccgccagct cgtaatctag cggaatttaa agcacaaaaa gcctatccga tgattcattt 56760 actaaatgcc cttgatgtag tgaattggga ttcagtaatg atcgataacc agaaaaagct 56820 ttgcttagtg gttatacgtg aatttaagtc tgagcgcggt gctgatggat ttagtaaaac 56880 cgaacaagag caatatcgtg tacttcgttt agagcaagag ggaaatgggg aatatattta 56940 ttccgttcag gtgtacacaa agggtgaaaa gggtaactgg gttggcggag agaagaagtt 57000 tccaacagat tacaacggga atttctggac ctatatacct tttacatttg taggtgcaat 57060 tgataattca gaagagatta aaaagccacc attacttcct ttggctaatc tcaatttagc 57120 ccattacaga gacagtgcgg actttcaaga gtccgttttt tatatggggc aacctcaata 57180 ttatgcgaag ggtgttaatt gggagtggta tgaccaagcc aagaaacgtg gcatctacat 57240 tggagcgaaa gtacttttgc ctttacctga aaatggtggt ttaggaattg tacaagccga 57300 ccctaatact cttgcccggg aagcgatgaa agataagtgg gaaaaaatga aggagatggg 57360 ggcgcgttta attgagaagg gctcgggaag taaaaagacc gctaccgaag cgaatagtga 57420 tgacgccgtt cagcattcag ttctttcgct ctgtgtcgtt aatatgaatg aagccttgtc 57480 agcagcatta cgatgggctg ctaagtttgt aacgcctaat gtggatgttc taactaaaga 57540 tgatttgatg ttcgaaatca gtcaagaatt taacaaacag ggttatttag ctgagttagc 57600 tcgacagtta tttgaagcag ctctacaagg ccgatcttca tttaaatcat ggtgggaata 57660 caaccaaaca ggtatgttcc ctaaacaaaa atatgaagaa gagcttcaga atgttgaagc 57720 agagcaagat gggactttaa atcaaaaggt agagtgagat ggcaacagat atcaaaaaac 57780 tatttgaagt actcactcag caccaggcct atctttatcg tgcttcatca aaaacggtaa 57840 atgagttatt ggctttattc aatgatgata cgagcaagat gctatctaag cttcgggatt 57900 tattggatga gcttaatgag tcggagaaag ttgctttagc tggtggtaaa tatacaactt 57960 caaatttaag ggaaattagg gatttgattg cccaatggtt tgccagtgtt aatttagcat 58020 tacctgaagc ttttgccgtt tctgctacgg cgctggctgt ttatgaggcc aattacgtag 58080 ctaagctcta tggagcaaaa attaataagc ctgatgggga aaaactattc ttatccgcta 58140 aaaaagttcc gttggcaggt ggcgctcttg tcgatgatct gctttcaaga attgctgaaa 58200 gtgcccgtca aaaggttgag tatgcaattc gagatggtat taattcaggc aaaactaacc 58260 aagaaattgt tcagcgtatt cgtggtacca aacggcttaa ctatgaagat gggatcttaa 58320 atggtaccaa aactgatatt gagcgaacgg taagaactgt gcgaagtcat gtagctaatc 58380 aagcctatct aaatagcttc aaccaaattg gctttgaata tgtccgattt gttagcgttt 58440 tagatggacg aacttctaag ctttgcgctt cattagatgg ttcagtgtgg gaaataaatg 58500 atccggcaaa gcgagtgccg ccgttacatc ctaactgtcg cagtatcttg gttccggtcg 58560 agaaggacgg tcaacttgtt ggcgaacggc catttgtaat ggacgaacgt agagttaaag 58620 acatccccaa agaagagcga agccagttaa taggacagtt agatgcaaac accacattca 58680 aagagttctt taagaaaaca gatgatttct ttcaaaggga gtggctaggg ccaaagcgct 58740 ttaagctcta taaagatggg aaatttgatt ttgataagtt ctttgatcct gaaggccgtt 58800 tctatagctt agatgatttg agaaagttgg atgaaaaagc ttttaaaaag ttgggtctgt 58860 aatttttctt atgttatatt ttttaaaaca tcagaattta tacaatatga aaacaatagc 58920 ttttgtatgt ctaaccctaa tttccatcac ttgtttagct gaaccaagtc aaaaatatct 58980 taaagaatat gatcgattgt ctgaagcttt ggagtcagca atggcaaatg catattcttt 59040 tgatcctgca actggtcaag taaaacaggc tactcaaggt ttagaagcta aaaataattt 59100 atgtagagct gcccaggcga aactaaacct caccacgttt ttaaaagaca atttagagga 59160 atctaaagag ctttataaat ctattgatgg tgcagagact ctagataaaa attatcttag 59220 tggacaacag caggaacaac aaaatctcgt ttcaaatttg aaaaaagacc ttgttggaac 59280 tggatttaac tgtgagtaat tattgccgat tacaggtaat tctaaactca cttaagacac 59340 aattttcacc tatataagcg cccaaatggc gcttttgtca tttatggagt ttggcttatg 59400 agtgaatcaa aagttagaca tttggtactt aaaagagttt cagataaatc ttctcatctt 59460 gctctttgtg acgaggaaac aggtattcca ttagctggat taaccgctgt aaaaatgaat 59520 tgtagtgttt ttgagggtcc agcgactatc acggcaacat ttgatgtagg tggtcctcaa 59580 ggcatccgct tagttggtga tgaacctaga caaaaggttt ggagtgcaaa ggaaacgtag 59640 cgaaaggcac tacaaatgcc tgaaaagcaa atcaatatgt cagatgctca atatattctg 59700 agcacaaaat gaattctggt gccatttctt caaattaagg tttcaagcca tggcaattta 59760 tggttttact tttgaaagat taaaagcaat tgcactcatc aaatagaact taatttttaa 59820 ccatagcacc ttcgggtgct ttttttgcga gaagaaaatg ccaagcccta ttatccaata 59880 tttccaatat gaacatttac ctgaacattt gcagcaagtt agtaagccaa ttggtgattt 59940 agctcggcaa atggatgagc aacttcctga cgggcctgaa aaatccacag gattaagaaa 60000 gctacttgaa gcaaaagatg catttgtacg ccaagcttta agtaaataat catttataga 60060 aatgaagcgt cctaaagggc gcttttttat tgcctgccga aagcggatgc taacggcgaa 60120 tccgggcgga tgcccatttt gtatatatag gttggatgac caatgaaact taaaacagta 60180 acaatcgacg gtaaagttta tgcggaagta gacggtgata agccgatcta tattcatgat 60240 gacggcaaag aaatgccaca tgatgcacca cactcggtag caacaattgc acgcttaaac 60300 aatgaagcta aaacacatcg tgaagccaaa gaagcagccg aaaaagcatt aaaagctttt 60360 gaaggaattg aagacccagc ggcagctaaa aaggcattac aaacaatcca aaatctcgat 60420 gataaaaagc tggtggatgc cggtgaagtt gagaaagtta aagctgaagc tatcaaagca 60480 gttgaggaaa aatatgcccc gattgttgcg caacgtgatg ctctagaagc ctctttacat 60540 aaagaactta tcggcggtgg ttttgctcgt tctaagtaca ttcaagacaa cattgcagta 60600 cctgtggaca tggttcaggc aacctttggt catcacttca aaatcgaaga aggcaaggtg 60660 gttgcatatg atccgaacgg cgaaaagatt tattcacgtg tccgcccggg tgaacttgca 60720 aatgttgatg aagctttaga gtcattggtt ggtggatacc agcataaaga cttaattctt 60780 aaaggtggta aaggaactgg tggcggtttt caaggtgggg gcaaaggtgg agcacctact 60840 ggaatgaaac gcagtgaaat gtctgtttct cagaaagcag attacatcaa agaacatggc 60900 aatgatgcct tcctaaaact accgaactaa tcattaaata tttggagata agtagttatg 60960 actacgacag ttaattcaga catgatcatc tataatcaat tggctcaaac tgcttattta 61020 gagcgtttgc aagataattt gaatgtattt aaccaagcct ctaatggtgc aattgtttat 61080 cgcaatgaga tcattgaagg tgatttcaat aaagaagcat tctacaaagt gggcggtagc 61140 atcaaacatc gtgatgtgaa ttcaaccgcc aaagtagttc cagagaaaat tggttctggt 61200 gagtctgtag gcgtaaaagt cccatataaa tatggtcctt atgcatcaac tgaagaggca 61260 tttaagcgcc gtgctcgtac accagaagaa tttgctatgg ttgttggtta cgatcttgca 61320 gatgcattgg ttgcaggccg attagagtac agtttagctt ctttaaaagc tgctatttct 61380 agcaatccag acatggttgc aaaaggtagt atcgttgttg atggccgcaa agcattgact 61440 cgtggtatgc gaaagtttgg tgataagttt ggccgcattg gcttatgggt gatgaactca 61500 gatacatatt tcgatattgt cgatgatgca atcactaagc aaatttatgg tgaatctgaa 61560 atcgttatct acggtggttt accgggaacc ttaggaaagc cggtcttggt gacggacgct 61620 gtaggtgata acgatgcttt tggtttgcag tatggcgctg tcactgtaac tgaatcacaa 61680 gtaccgggct tccgagctta tgacatcaat gatgaagaaa acttagcaat cggtatgcgt 61740 gctgaaggtg catttaactt agatattctt ggttatagtt gggatacatc gaaaggtgaa 61800 aatcctgacc ttacattact tggttcaagc gctaactgga tcaaatatgc aaccagcaac 61860 aaaatgacag caggtacctt acttgattta tcgggtacag cgacaactgg ttaaaaccta 61920 aaaattaaaa cctaaggggg ctaataagcc ctctttttta ttattaagag aaaagcgcca 61980 tgaagattat ctatacacgc attgcagcag cggctgcatt agagacaggc attattgcta 62040 accctgacta ttatgaaaac ccaaatttga aagcaaaaga ggtaattatt tacggtaatt 62100 atccaaagat tcaaaaggat tatgaatctt tggaagttcc agttgaagtt cgtaagttgg 62160 aagtgccaca aaaaacgact ttggccacag taaatgtcgc agtgggaatt acccctgaac 62220 ttcaagctgt gatggatgat gcaaaagctg aatgtgaaaa ggtagttgaa gaaaacactc 62280 agcttaagca gaaaattgcc atcttagagc aggccggtgg taaccagtca gagttgttat 62340 ctgaaaattc acgattaaaa gatgcagcag tcttagcaga taaagctctc aaagatgctg 62400 aagctcaagt ggtcggtata aaaactgaat ttgaagcttt taaaaatgat attcctgcaa 62460 tgcaggcacg tattgctgaa ttggaagctg gaaaagcggc agaaaaccca gctacagaaa 62520 cggcagctaa tgattttgaa aactggtcaa atgatcaatt aaaagagtat ttggctagta 62580 aaaacattgg ttacaagccg tctgcaacaa aagcagaact tcttaaatta atcccgaagg 62640 aataatgcaa tgagctttat tactgtagat gacgcaaatt caattttggg cagcgatttt 62700 gcaccagaca gtgataaggc tcgtctggtg aagctggcaa atgtgtggat gaaaaacaga 62760 attggttttg taccagatcc aattgatcca cttcttaagg acgcggcttg tgaaattatc 62820 aaaggaattc tggccaaagt aatttataac ggcaaagagc agcagttgaa gcgtaagaaa 62880 gttaaggctg attctgttga gtcagaaaaa gaatttcaag acggatctga agcaatctct 62940 agctttgaac agatagcaat tgattttatt gattcacttg atttgaaaga tccaaatgca 63000 agttttaatg gctttggcat accactttac agggcatgat atgggcttac gtgacgaaat 63060 tcaggcagac attgctgaag catttaatga tgatttagca gatgccattc atacctttac 63120 atgtgagcgg atctctaaaa cgaattggga tcctaaaact gaaacttatg ttgaagttaa 63180 agaaaactat tctggccgtg gcgttctgtt tggctcatac agtcaatatg agatccaaac 63240 acttggagta ctggccacgg ataaaaaggc aaccgtgctt caaaatgaag taactatgac 63300 tccaaaaatt gaagatgaat ggttaactgc cttaggctca ttccgggtaa ttaatattca 63360 acaggatcca gctaatacta tttggaaatg tcagcttcga aaagtgtagg ggctaaaatg 63420 gttaatcctg attatgttcc tgaatggtat atctcgcctt ttcaacatgt gcagtacacg 63480 cttgctcgaa atcaactaca catggatttg ttatttgaag atatggataa ggccgatcaa 63540 tttttggata tgggagcgga tgcgcaagtt agtacttttt ctgatggtgc atatgcaatc 63600 gtccaaattg gtgatacggc ggataaagac cgaattcaag tttatggatt gcttttacat 63660 gaagctgttc atgtctggca aaagattaaa aagctcatgg gtgaacgaga accgagctct 63720 gagtttgaag cttattcaat tcaggcgatc gctcaagacc tttttaaaat gtatgaagaa 63780 agcgaggtga atgatgggat ggaaggggaa aaagccaact gaatttagtt ttgatgtgtc 63840 taaagcagca gaagaccagg tgaagcatat tgttatggat actgtgcaat ccttagttaa 63900 tttaagtcct gttgatactg gagcataccg tgcttcacat attgtttcgg ttggatctgc 63960 tgatttcggc gtgcgtgaac ctgaaacaaa ccctattaac gacgcagcga ttcaggcaat 64020 gaagattaag ttaggtaatt tggtttatat ccagaacaat aaagcttatg gaccgcgctt 64080 agaaaacggc tggtctgatc aagcaccaca aggtatttat ggcctcacgt ttaactttat 64140 ttctcaaaag tacggtggct aaaatggcaa tgactttaga gcagacaagg caagctatta 64200 ttgatcgcat gcaaagcttt acgggtattg ctcaagaacg gattcagtat ccaaatgcac 64260 ctgactttac tgttccaaca aaaggtgtat ggtgccgttt aacgattgca ggtggcccga 64320 gttttacctc aggcattgca gataagccat gtacacgccg taccggtaat atcatgatcc 64380 aatgctttga ccgattacat actggagaga aggccgtaac ggttctaagc gatgcattgc 64440 tggctcattt tgaatatttc acaatcgaac acttagaatg tttgaatggc caatctattt 64500 atgcgggtaa agatgctgat ttcattcagt ataatgtgag cattgggtac aaggtgaatt 64560 gatatgtcat gtatgctgac tttagaagaa atcgaaatta aacggcaaga actggaacgg 64620 catcttgaag atgttatgtc tgttgagttg agcaaatggc aatctgaaaa caagctatgt 64680 gtttctgatg tgaatatacg cttggctaat gttgttagtc tcggagggcc taaacataac 64740 gttgttactg gagtaagtgt cgatttagat aatgagcttt gagttcaaga aaaagctact 64800 gcaaggcgat tatttttaat gacctcagca tattatcatt tgtgattaca ttctgttaca 64860 gtaatggaaa tttataacaa atggtaaaac atgaaaaaat caactttagg ctggggtgcc 64920 gcaggattag tagctttagg gatttttggt tcaggcaatg ataactctcc aaaacaaact 64980 tcagactcag aaaatgcgca gagtgcagta gaggaagtta tcgaatcaaa atatatcaac 65040 actaattctt taaatattag agataaacca aacggtcaag tagtaggaaa gttaggacgt 65100 ggggaaaaag ttgatattta tgagatgaaa ggaaactggg cacgtatttc cttaaattcc 65160 tcatcacctc agtggttatc aacaaagcta ttatgtgaaa cggatggctg ttttaaacaa 65220 aagtctcgat caaccacgtc aaataattat caggccttaa aatctcatcc tcatcattct 65280 gaaagaaaac agaaaaaaac ctactacgat agtgattgtt catgtgctgt ggtggattat 65340 tgcgtgggtc ctagaggtgg gcactactgt attacgagtg gaggaaacaa gagatataaa 65400 cctagatatt aattaatttg aattatgaga cctccatttt gagaggtact ttatgtctta 65460 atcactacca cctcatcggt ggttttttta tgtctatagg aatcacttat gagcaatttt 65520 gtatttaagc gtggtgacac tttcaactta aatcttcagc tagttgatat ggatgaagcc 65580 ctgcaatatc caccagatga tgtgcgccgt gcaattgatc ttacaggtta taccttcact 65640 tcacaggtta aagctctggc tgatggtgct actgtggcca cattaacttg tactgcatta 65700 agccaaagca cacagaaagg gtggctgaac attaaatcta gtgcaagcac tgcaacttgg 65760 cctttagggc tgtgtcagat ggatattaaa gctgtagtta gtggcactac acagcacact 65820 gaaactttga ctttccaagt gattgacgga gtaacagcat aatggcaaat cttgttttta 65880 aatttagttg ggatcatcgg ccattcccat ataacgcctc acagggcaag cggcagttta 65940 tgttgccatt tgcgtcaggt attcccaatc tggcacccaa cttttctcaa gtagttggta 66000 ctgcagctat ctctcaaggt ggaacggggg caactacagc ggctggtgca cgagctaacc 66060 taggcgcagc tgcaagtggg gtaaatagtg atattagtga gcttaaggga cttacaaccc 66120 ctttatcaat ttctcaggga ggattaggag ctgataatgc acagacagct agaatgaatt 66180 tggggttagg aactgctgct gtactagcgt caacaacaag tcaatatgat cctacgccgg 66240 gacgagcact aagagtcggt gattggggga taggggctga aggttctcgt gtatctgata 66300 tggttgctcc tcttaataat ggtttttttc gaacagatga cactttaaca aatgatactg 66360 gtaatagtat tggtccttat ggtttctttt tacactgtac ccgacgctca atgggtttat 66420 atacaaatgg aagtcattca tttcagcttg ggaaagctgc ctcatattct gccctgaagt 66480 atcgatttaa taatagtggt acttggtcta attggtttaa tttattgact gcacaaaata 66540 ctacaactga tggaaatggt tttattaaag ccgcttcacc agtcgttaag cttttccaaa 66600 gtcatattga gctaaataac gatgctgcca agcaaccgat cacttttgac aaattaggca 66660 ctggtgacta tctgattaag ggctctttag gctttgcaca ggaaggttgg tatatcgaag 66720 tacctaagga tgccaacggt aatacggtag tagcagttga atattcaacc ttagaaaatg 66780 gtgatctttc tattaaaact tataaacgta agtttgatgt ggaaaaggca gccattgtag 66840 ctgatctcga aaatccactt gatattccag aaggccgctg gattgatatc cgtctgcatg 66900 aagaacctga accagagcct gaagagccgt tgagtgaaac accagtggat ttccagccga 66960 ctaacttatc tcaggcagtt gctgcagcca tgaatggcgt ggaaccgcca gaaatctcag 67020 aaacagacga aacactttaa taacccgctt aaacagcggg ttttttattg cctaaatttt 67080 ggagaaccat aaatgagttc aggcgcaaaa attcgattat atgcttgtga ggaagcagtt 67140 ttaggaacaa ctcctgcaaa tccagtctgg tacaccgttc gccgtgttac tgatagtttg 67200 actgaaaatg ttactactga agatagcagt gaagtagttg attcacgttt tcgccaaggc 67260 gctgttgtaa cggaagccga agtaactggt caactagagt ttgaattatc actaggtacc 67320 tttgacttat tcttaaatgt tctcgctttc aataactggg ctgcaaatgc tttaagtttt 67380 ggtggtggag tacgtaagtc tcttaccttg gtaaaagtct ttgaagatat tggtcaagtc 67440 tttatttatc gtggtattca agtgaataca ggtgaaatga cgatccagac cacaggcaaa 67500 atcactggta actttggttt agtaggtagc tcatttacgc gacagcaggt taatcctgtt 67560 acaaatccta ttccagcatc gactcgccct ctggtgagta tgccaaatgt tgaaaagcta 67620 cttattaatg gtcaatcaat tcagggtaaa gcttgtctgc agacacttac catcaacttt 67680 agtaataatc tggaagcgat ccgttgtatc ggttctggta agtacacgcc tgagttttac 67740 ttagagaaaa tgatggatat tggcgtaaat gctaatttca tgttttcagc aacatctgct 67800 tcttggattg atgctattaa aacccgtgat gtatttacat tgaccttcga tattacagat 67860 accaaaggca gtaagtactc gtttaacttc ccgcaacttg aagttaagga agcaaatcac 67920 cctgatggtg gtggcgatga cattattaca atagatatca attttgccca agtgcgtacc 67980 agtccaacga ttgtacgtgc tcttgtgtaa tcaacttatt cagtaacaaa gcctatggaa 68040 acccatgggc ttttttattt ctaaaaatta gaggttgtta tggctttaaa agtcggaatt 68100 attaaaagct cagacgtatc aaaatggtgt gaatacaagg gtgctgatgg cgatgtacag 68160 gctgagttca aagtccgtgg tatcgcttat aagccttttc aggtagctat tgaacgggca 68220 ggaaaccaga tctcgtctaa aggctatgat gtgatggtca aagatgaaga tgccaagctt 68280 taccacgagc ttttaatgga tgcatgcgcg gcccacttaa tcgaagactg gaaaggtgtg 68340 gtatttgccg aaatcgtaga cggtaaaact gttgagtccg aaaagccata tacacctgag 68400 aatgcctcaa agcttcttaa tcttggtgat attggtattt caatctggct attcattaaa 68460 gaacaggccc agaagattca ggaagacgca gacaaggaca aggctttaat tctgggaaag 68520 tcatggagct ctacaaatac caaaaaacgt atgcgtcgaa aacgccgcac gaaatcgagc 68580 aaatcaagtt cttaggcggc cgtattccgg atccgccaga atattcgtat gcggctgatt 68640 caattctttc ggcatttagc actatatgtc gatccagacg ttatgagcaa agcataccgt 68700 tatctttaga tcagcaggct atcaatgtct atgctgagca taatgatttg ccagtggctg 68760 ctcatatttt taatgactgt atttttgcgt tggataattt gtttttggag gagtgccata 68820 agaagatatc aaccaaaagc aaaggtaagt gaccaaatta ggtattgcca gggactgaaa 68880 agcctaattt ggtcaaaacg tcaaacaatt aagcagttgc tcttaaacgc gactcaaaat 68940 aacgcagtcg atgttacaaa atacttgatc tggattgaca gaaaattacc tttaaggtgt 69000 tgcgcgtgat tatcaaatga tgaataatca ccttaccgtc aatatttgac ggttcagcat 69060 tcttttactc tttccaagaa ccttggtgtt tgcttgtatg tgtttaacat taactgaagc 69120 taaacaaaaa cttagagcat ttgctagaga tactagcaaa atcaagttaa ctgcacatgc 69180 aaaagaaaga atgaaagaac gctgtatctc tatgaagcaa attatttgct gttttgagca 69240 tggagatatt actgaggggc cgtacccaaa tactcgtggt gattgccagt taaatgtttc 69300 tgttcgcact gcaggcgaat acataacaac agctgttgca atcaagcaga gcgagaacgg 69360 tgaattctca gtagtagtca ctacatttag agagtaggct aaattatgta tcactatgaa 69420 gaatgcggtc tgagcaatat ttggctgcgc aatggattta caattgaaaa tgatgaagac 69480 tatggtgaac tcgtatctat tgaatctgtt catgagcttc ataatgccat tgggttgttc 69540 ttaattacgc aaaagcctga cttgaatggt gaggaaattc gttttttacg taaagaacta 69600 aacttgtcac agaagaatct tgctgggctt ttaggagtca gtgagactag tattagacat 69660 tgggaagctg atcgcggttt aattggtaaa cctactgagc tattacttcg tgcattatat 69720 aaagagcatg ttcaaggtga tggcaaacta agaagtatga ttgagtcatt aaatcatcag 69780 gaacgaactt tagtaccaag tgaaattagt ttttcatatg gaaataacca ttcatggcat 69840 caaaccaatt gtgaaatagc ttagttagtt ttatttgata gaaaccacct tcgggtggtt 69900 ttcctttatg tgacatttag taaccagttt gttaaagtta gtacacttta taacaaacgg 69960 tgaaattcat gaaaaaaata ttggctgcgg gtttaattgg tcttgggttg gtggggtgcg 70020 ctactccagc ctataattat caagctatac ctaaaaatat aagcaaaccg ccaattggat 70080 cagttaataa agcatttgta ggggatcaaa tgcttgaaca gggaatggtg gttgatcgtg 70140 aagttctaaa cgtccctgaa aatattaaaa ttagttttgc ttattcactt acttcaggca 70200 tttacttaaa aacaggcaaa aatgaaaaag ggcaatattt tcagccattc aacactgtca 70260 gtggtggggg gatggttcag aaaaaccctt tagctgaccc atttaaagta gttatgttag 70320 atactgaagg taagctctgt gtagtaacag tatttaatgc aaaaaactgt actgataaac 70380 atcaagctac tatgaagaca gtagcaattg catcagataa ttccttccaa caaacattaa 70440 tttatagtgg aaaatttgga aataaaatta atgtcgggta ccgtgaattc tcaagtaatc 70500 aagcacgtcc tgcattcaat aatgatgttg aatatgattt aagccaatct aagcaaatag 70560 gttataaagg tgctttattg gaagtaattg atgccactaa tcaagatatt acttacaaag 70620 ttttgaagaa ctttaacaag gtagattaag atgagtgcac cacaatataa accaatgaga 70680 gaaagtgaag tttgtaatgc tatcgggtgg gtgttaatag ctcttggctt tatcgcaggt 70740 tttttattta ttcttgcatt tggtcgaatt gaagtagctt cttactatgg taaagaaacg 70800 gtttggtctg gagttatgat agcaacagga atcggaatta tatttaatgg attccttgca 70860 ggctacttat ttcaaaaagt agctagtatt cttcgttacc atgagaataa ataatatctt 70920 gcataagcac cctaggatgc tttttaaaat tggtttaact accctgcttg gtaattatat 70980 ttaacttaaa aagaactacc cactcattga gtgggttttt tattgcctag aggaaagtaa 71040 aatggcacaa gaatcccgtt tggtcattgt tattgattcg caaaatgctg aacgtaatgc 71100 gcgtaatcta ggcaatgaac ttgttagcat tgaacgtaaa ggtgaatttg catctaagtc 71160 tatggacagc ttgtctgtag ccaccagagc tttagctgga cacatggctg gtttattaac 71220 agtaggttca gccatttcaa agatggatac atatactgga ttacaaaatc gccttaagtt 71280 agtcactaac aatcaagttg aactaaataa agcaacggaa gacactttcc gaattgctca 71340 aaaaacctat tcagcttggg attctgtgtt acaggtttac cagcgtttta gtgataatgc 71400 caaaacttta aacctcacaa tggatgacac agcacgttta actgaaacag tttctaaagc 71460 tgtagcaatt agtggtgcaa gcgcagaagc tgctgatgca gctttagttc agttcgggca 71520 ggccttggct agtggaacgt tgcgtggaga agaacttaat tctgtaatgg agcaaacccc 71580 agcactagca aaggctattg ctaaaggtat gggtattact gtaggtgaat tacgttcagt 71640 agcagctgaa ggaaaaatta cttcacaaga aattgtaaaa gcgcttagaa atgtagaatc 71700 tgatgttgat gctctttttg ctaaaacaga tatcacaatc gggcagtctc tcacactcct 71760 aaacaacgag atcacaaaat ttgttggcga agcaggtaag ggaagtggtg cggcacaggt 71820 attagctgga tcagttcaaa ctcttgcaag taatttagat ttaattgctg atggggcttt 71880 agtagttggt attggatata tcactcgtgc aattttgatg aagagcgctg ctattaaaga 71940 gggaatggct tcaactttag cgagccgcca agcatctgta ttaaatgctc aagcagaata 72000 tgcagaagct accgctgctt tgaatgcagc aaaagctcat ctcgcgaatg tgcgagcaac 72060 aaatgcagaa acccaagcta aatttggcgc aacagcggca gcaactcgat acgcacaagc 72120 acaggcagca gtaactgctg ctacaaatgc acaaacagca gctcaaatta agctaaatac 72180 tgcaacttca attgcaggga gactagctaa aggggcgttt ggattaattg gtgggtgggc 72240 tggagttgca acattaggag taatgggatt agcggcagcc tattcttatt ttaataataa 72300 ggcagaggag gcaaagcaaa agcttgctga acaagctaaa gttgctgaga aagctgatga 72360 ggagttaaaa aaattaactg gcaatgataa ggctaaagca gttaatgatt taactactgc 72420 ttttaatgca caaaataaag cattagagaa atcatcgcgt gctgtagggt ctgcattaat 72480 tgatatcgag aactatgcac gaggaaatag ggaggttgaa aaaatttccc aagaagcgag 72540 aactggaact atcagctata cagaagccat tgaacgtcta aataaaatta agttgcctac 72600 agatctatat gaaaatctga aaaaacaggc tgcgcagtat gatgacaatg catctaaagc 72660 aagtttatca gctgagaaac ttaaattatt aagagttgaa gtgaaacttg gaggtaatga 72720 agcacaaaat gcggcaattc agcatcaaaa acaagcggat gctttaggaa atactgctac 72780 tgaagcagaa aaggcaacta aggctttgca agattatcaa gccaagcaaa aagatagcgt 72840 tattgattca atctataaat caggttggct tgataaaggt tacactgttg ctcaagctaa 72900 tgccatttta gaactgcaaa aagctaaagg aatgagtgca attttgtcta aagatgaaat 72960 tgatagcgca cttagaaatc tcaagatcat cgaagaacaa caggagcgag aagataaatt 73020 aactgaagct aaaagaaagc agacgcagga aattgaaaaa caagcaaaac ttactaaacg 73080 cttggtcggt atttccggtc aatccggtat tggtactggt ccacatcttg acgtccgata 73140 tggtggctca ttgtcaggtc agaaagtttc taatgaacat ctggctcgat tacaggcggg 73200 aggaaaacct ttaacttcct acaagatcag ttctaattat ggtccacgaa aagccccaac 73260 taaaggggct tcttcatttc ataaaggtat tgatttttca atgcctgaag gaacaccaat 73320 cacgaccaat gttgctgtga aagatatcaa gacatggtat gacagcaagg gaggtggtta 73380 tgtcagtgaa gtgatctttg aggatggagt gtctcttaag cttctacatc aatctcccaa 73440 gatgcagagc aaggtgaaag gtggtgcaag taaaggaagt gataaagcag ctggtgatat 73500 tcaatctcaa cttgaacgtc aacaggattt gcaacggtca cttgaaaatg aggtggctag 73560 tgaagtcgga cggattaaca ataatagaaa ggcaagactg gaggatgttg ataaagcaaa 73620 ctttagcccg gaacgtactg cagaaataaa ggcggaaata aatcgtcgtg cagataatga 73680 tattgctata gccaaacaag cccttagaac gaaattggaa gactataagg agttccagaa 73740 aaccgaggaa cagttactag aagagtcctt taaccgtaaa aagttcaatg cagctcatga 73800 ccttgaatta agtaagtttg agcagaagca agctgttgaa ttgctggaac agcaaaaaca 73860 gcaagagtta gggttattaa aactagctca ggaacagcgg ttgtttcaag cccgtttatc 73920 tctgctttct gaaacgcaag ccatgcagga acgttacaga ctcgaacggg aggaaattct 73980 taagaatacc aagctttcta tagaagagcg gcaaaagcta atcgcattat ctaaagccaa 74040 tcaggataaa gagacacgcg ataaagtgaa taatgctgtt caaaactggg gtggtatcca 74100 agcggatatg aatggtaccg gagaattttt cagacaggat caggaacgat ttagccgttt 74160 aaatgctgca aatgatttag cagatagtca atttgctgct actgatcttg atgaaaaaaa 74220 tggtttagat gttctaaatg cacacatgga agcaggactc atcaagcaac aggacttcga 74280 aaaccggaaa acagctatca ttcaagctgc tcaggaccaa cgcaatcaga tcgctgccga 74340 atatgctcag aatgctcagg atattgaaga taagtatcac caagatcgat tgaatgctca 74400 aattgctctt ggtggccaaa tgatgggttc actcacatcg atgtttggtt caatgtttgg 74460 cgagcaatca aaagcataca agatcatgtt tgctgctgat aaagcttatg ccattgcagc 74520 tgcaggtatt tctattcagc aaagtattgc aaaggcggct agtgttggtt ttccagcaaa 74580 tatcccatta attgcaagtg ctattgcaca aggtgcaagc atcattgcaa acatccgggc 74640 aattaaagat caaggctttg ctgacggtgg ttacactgga tcaggtggga aatatcagcc 74700 tgctggtatt gtccataaag gagaggtggt ctggtcccaa gaagatatta aacgctgggg 74760 cggtgttggc ttagtcgaga aaatgcgtaa gagtgcaaac cctgaagctt ttctcaataa 74820 caatgccttg gcagatagtg tcatgcgccg tgcaatgatg agctctagtg cctttataga 74880 aagccaaaag caggctgaca tctttaatca accggttcaa gatactcaga ttatctataa 74940 gggtaataga gacacaccta aattagcttc ttctggaaat ttagacttat tccatgatgg 75000 caaggtctac ttctcatcca atggtttagt tcaggatcgt tcaaatctgg atgatgttca 75060 ggactttact ttaggaagta cttcacgccc tcaagctgag atgatgcctt caattgagcc 75120 agcttcaccg acaatcaatt tcaaaattga agtgattaat caggtgagtg gggcgacagt 75180 tgaagccgaa caactggacg agcaaacagt ccggatcatt gttaaagatg aactggataa 75240 gcagcttcca agaacggtac ctaagcttgt aagtgatcaa atcgcaaatc caaactcaac 75300 cattagtcgg tctttgactg agaatacgac agcgagaaga aatcgtactt aataatttga 75360 acccttttcg gagggttcat tttcataata tttaaatttc aaggtgatag agtctgttgg 75420 cattaaaatt gatggttaag acatgaaaaa aataattgta atttctacaa cacttttagg 75480 ccttacggga tgtgccattc ctgcggtaaa taatctcgta agatccacaa atatgtatca 75540 ggatgatgtt tcgggaaata ctgcaaattt aagggtttat agaagtaata ttcccatggt 75600 gcagttctat attacttatc aaaataatga gggtgaaaaa atttcaaaaa acctaataac 75660 taagcagatt tcaaataatt taacaaagta tggctctatg catgagccca aaaaattaaa 75720 tatgcctaaa cccacaatca gtttaaataa tggtgaagag ttttttgagt ttaaagtacc 75780 cgcaaataag aagttaactt tcaggcttac ttctgttatt gggtcaacta ctatgtatag 75840 ttgtgatgta aaaatggact atcagttgga aagaaatgga aattatgaat tgatccgttt 75900 aaaacagatc aaagattttg tgaatccagc tttactgact gaaccatctc aagatgaagc 75960 ctactgcaag tttgtagtaa aagagatttt tgaagatggt aaagaaacta ttattaaatc 76020 gatttcttaa tgttaaatcg tttttgtaat taatttaaat atctaaacct tatttcatca 76080 aaccaccttt cggggtggtt ttttattacc tgaaggaaag ttatgtacaa gttaaagcta 76140 aatcctcaga ccagcggcta tggcgtaaca ccgggtgatg atgtgaaacg tcagcagatg 76200 gatggcggtc gtggtcgcta ttacatcgat gtgaaacgta acagccacat tgttgatgtg 76260 aactggaact taagtaaaac cgatttcaat aaaatgatgg ctttctggcg ggtctaccag 76320 aacaaaccag cctcatttta tgcggatttg gtgattgatc agggaacacg tcagcaatat 76380 ctatgcaatt tcattccaaa ctcgttcaag accaatgaag tcaacggcaa cttatatcgt 76440 gtgaacgcgc agctcgaagt tgttcaaaac cagcctaacc tgaatgccga tatagcatta 76500 attaaagatt gggaggtcta atggataacg aatatgccaa gttctttttc aatcggaaag 76560 ttgatgtcta tcaactggag tgtattgagc tatcacatcc ttcttttatg aatacttatc 76620 gggtagtccg taatgatgac cgaggtgtct atgtacaaca taaggaagga tccggtcagg 76680 tctattatga gttcttgcca gtctctatac aaagatccgg aatgcttggt gatctggacc 76740 agacattaac cgtttctatc tctggtctag gtgatgtgat gcctgatgag tttgaacggg 76800 taatcgaagg gcaatatcca gatgtaaagc caaccgtaaa ttaccggatt tacagttcag 76860 acaatctgaa ctctccaatg ttttatttac ttggactgca actctcaagt gtcgccatga 76920 accataaagc tgtgacattc aaggctgaat caccacgatt aaataccact aaaactgggg 76980 acatttttgc actggatcgc tttagtggtt tgaagggggc tatatgaaaa gtcatgatca 77040 tttgctcgat aggcaatatg acgaggatca ctacaactgt gttcactttg ttcatgaagc 77100 tgcaatggac ctatatggca tagatcgggc tgaagcgctt gaactcttta tgcagcctaa 77160 gggcaaaatt acttttttat cttcacggtt aaaactttta aatccgctac ccatgcccaa 77220 ggaaggctgc atagtcgcct tccatccgag acaaagaaat aagcccccgc atgtggggct 77280 ttttcgtggg caaagattct tcacctcatg gaaagcggag tcacttattt gcctgaagag 77340 gttgtgatgg aaatggggtt taatcgggtc agttattatg attaaagtta tttataaaaa 77400 agacgctttg tctgaagaaa agacaattga gcaggctcaa accattgggc aatggctcac 77460 ttcaaaatat gaacatatgc ctgagcatgt ccgtatcttt catactacaa gcaatatgga 77520 tcatgccgaa atttcatttg cgaatgaagt cacaccaaag aatgcatatg acttaaagca 77580 gcttgatttc ttaccgggca cttttatcgt agttgagaac cctaaatggg tcgcggctat 77640 tgtttcgatt gtgattagta ttgcgatcgc atttttaatg ccaacgccat caatagcaca 77700 aacgactcaa aatactaacc agtcttcttc agcaaacaat gaactttcta accgggaaaa 77760 caagatccgg gtgaatggtc gtattgctga taactatgga gctgggtgga atactcccga 77820 cctaatcgca gtaccttaca aggtatatga aaacaacgtt gaagttgagc atgtagtggg 77880 ctgtattggg cgtggacact ataaaatcaa tggagcttat gacggtgaaa ccaatattgt 77940 cgatattgct ggcgcatcgg tagaagtctt tcgaccaggt gtagatattg tttcaggtga 78000 gccatatttc tcgcttggta ccgaaattac cacgccgcca ctaacggttc agcatcaaac 78060 ttctgttaat ggccaagttt tacgtcctgc tgatacacaa tctttagaag gtacgaacta 78120 ccttcatttt gcatatccaa acgagattct tcgggcaacg gcaaacaaca cagatttaac 78180 cactaagttt gtaagtaatg accgcgtaga aatcaccaat gcctcattca cgtttaatgg 78240 ccagactttt gatttaaatg gtacttatag cgttctatcg gtagcagatg atcgaatgac 78300 gttatcaaat ccggcggccg ttaatgctaa ctggttaaag cttaaagagt taaataacca 78360 acaaactgca gctttgtcac caaagatcag ttcaataggt gaaaaatgga ttggtccatt 78420 cattctggac aatgttgaac gtagccgggt gctgtgtaat tttgtggcca caaatggact 78480 ttataccgtt tcttcaggtg ggaatcaggc cgctgttaac gtcacgattg aagttgaagt 78540 aacaccggta aatgaatctg gtgcagcgat tggtaatccg atgctgaagc agatcatttt 78600 gaaaggttcg gcaaagtcgc gtcaaaccgt tggcgcaacg ctggatatgg tgacatttca 78660 aggtcgctgt agtgtccgtg cacgtcgttt aacaccaaca ccggcggtta caacggtagt 78720 agatgaagta aagtggcagg cgctttatgg tgcttatcct ttgcaaagca cagtgtatga 78780 acatgaaacg gtttttcgtg cgcgcactta tgcaaccact ggagctttat ctgttaagtc 78840 ccgcaagatc aattttgatc ttcagcggat gttaccgact tttaaaaacg gcgcaatgac 78900 gacagagcta tttccaacat caagctttgc tgatgcattg gtttcaatgg cactggatga 78960 caagataggc cgccgtacga tcgacgaaat agatctggaa aatatctatc ggacttataa 79020 cgatgtagtt gattattttg gtacaccact tgcggctgag ttctgtacta cgattgatga 79080 tacaaacctg tcttttgaag agctggtcac caatctttgt gatgccgtgt tttgtactgc 79140 atatcgtcaa aataataagc tcaagcttta ttttgaacgt ccaactgata actcggtaat 79200 gctatttaac ttcaggaata ttattcctga tagttacaag catgatctta cctttggcgt 79260 gatggatgac tacgatggac tgatctatga atacacggat ccggccgacg atagtcgtat 79320 caatatctat ctaccggata aaggggccaa gaaccccaaa gaggtgaaat ctgtaggtgt 79380 gcgtaacaag tggcaagctc attttaatgc gtaccggctt tggaacaagc ttcgcttcca 79440 gcgcaaatcc attacctttg atgcggcacc tgagtcagaa ttactggttt tacgtgaccg 79500 gatcgctgta gctgattatc gcaatggtat tcatcaaagc ggtgaggtgg tacagcaaga 79560 aggtttaatt ctcaccctaa gccatgatgt cgatttcatt gcaggcaaga gttatgtgat 79620 ttatttgcaa atgggggatg gtaccgtgga cctgattccc gttacgccgg gttcagccaa 79680 gaacaaagta gttttagggc gtttaccgaa cggggcctta aagcttagtc ccgatgactt 79740 tgtgaatact atctacaccg tagttaatga cgataccaaa ggctcactgc cttatctggt 79800 tgcaaaaaga gaaccggctg accagttctc taataccatt actgcaatta attacgatga 79860 acgttattac ctcaatgaca aggactttat tgatgtgccg gttgatgatt caccgattta 79920 cattcgatat gaccagctgg atattaatct ggcacgttta tatcagatgc aaagagggga 79980 tttgccaacg actggagaaa tcagttttgt agttgaagca ggtgcactag tttcaagttc 80040 aagttcttat cgaccggaaa ccagatttgt ctataaattc gactataact ctagtcctgc 80100 aaaacgagag tatatcgttc cagctgcatc agaattacct gctattgata ctggtgagtt 80160 cccacctgat ctcgtggtaa atttgactat taaaggtgct gttgttggac gtggtggaga 80220 tggcgggttg ccacatttgg catttggtgc atggtctacc gatccggatt ataactttac 80280 taaaacccgc cgtgacggtt ttcagggagc acccggttta ttaaaccggc acagtaaact 80340 aaacctgatt attgatggtg gaactctggc tcgaggcggc tcaggtggtg gagcaacacc 80400 aagcggtatt tatacaggat tatcgtatgg agttcagggt attcccggtg gagctggagc 80460 accttttggt cgggttatga ccggacaacc tattactaac gattcacaag actggcgttg 80520 gtacttaaat ggtgacttta tggttgtcaa agtaaccgat gccgaagctt cggtacccgg 80580 taaaggttac cgaacccaaa atgatcgata tggatctcca ttgtctggtg atggtggagg 80640 ttggggccag cgcggtacca agtccaccaa tgatggaaca tggaactggc aataccatgg 80700 cacaactgaa ggccagccgg ggccgggtgg acctgcaatt gttggggtgg caccacttac 80760 aactcaattg atcaatggag ggaaaattct acaaacactt taaatcttaa aagaactttg 80820 agcacccaat tcgggtgctt ttttattgcc taaattttct ggagatataa atggaaccag 80880 tttcaacaag cggtttaaca gcaattttaa aattttatgg tgcagcaatt atggtgacgt 80940 tagcggttgc tttagttgca gcagttgtat tgatgacacg tatgccacga tcacctcaag 81000 aatgggctgt aggacttatt tgtacggttg tatcaagttt ggctggcggc tcattcatta 81060 ttgtgaagtg ggggcttcat gaatgggtta ctgatgtatg gggaatgatt gcacttggtg 81120 ggttcttctt tgtttgtggt ttacctggtt gggctttagt ccgttggatc tttaatttca 81180 tagataaaca ggaagggaaa acgattgttg aagtgattaa agagtttaag aaagccagaa 81240 aagacattga aaacagctaa tgccgccttc gggcggtctt gtttagaagt acacgtataa 81300 gagagaaatt acctgttgac actgcaagcc gctgactact acgaaaacct attgacgacc 81360 aatattatga aacgaccacc ttcgggtggt tttcctttat gtgacattta gtaaccagtt 81420 tgttaaagtt attatattta taacaattgg tgaaattcat gaaaaagata attttaggga 81480 gcatgttagt ggctgttttt tccacatcat tttcacatgc tttagctccc aaaaatggag 81540 atgagccaac ttattgtgag cagattgttt cggtccatgg tttattaact agagcacaat 81600 ttgaatgtgg atatagtgaa tataacaatg agttaatctc agattcagcc aagtgttttc 81660 agcatgaact tggcgaagaa tatggaaaaa aagtccttat atttggcatg aaagaatttg 81720 accgaaatgt aaagaaagac gggaagaata agatttgtaa tagtttatta aaagaatttc 81780 cagagtatgt aaggaagtaa ctgatgaaaa agctactacc aattgcattt ttactcacag 81840 catcatttgt aactcactca gccgatacta atgataaaca ctgtagagat gtgaataaac 81900 ttgctgaaaa tgtcatgctc tttaggcagg aaggggtttc tgtggttaga caaatggaga 81960 tgatagagag tatcaaacca agcagggatt tcaaaaggtt aatggagatg atggtcgagg 82020 aagcctataa agaaccaaag tttggatcag aagagtataa ggcggaagca ataactgaat 82080 ttgcaaacaa ttggtacatt cagtgcaagc aagcaaatcg aaataaatag agcactttaa 82140 ggtgctctaa ttattgaaat tgagaaaagt ttataagtag gtttttatga gaaagattat 82200 tttattgggt cttatttgcc ttcctgtatt cgcatatgca aatagttgcg aggtggcaaa 82260 aagtaaaatt aatataagcg gtttagcatt gggtaaatcc attttatcac tgaaagcaga 82320 acatcctaaa aatttgagca tagatcatga gactaataag gcaaatatta actatgttca 82380 ctctaatgaa tttgaggatg cttttagtgg aacaccagct accaatgcgg gattcatttc 82440 ttttgatggg aatacaaagt taattaatgc gtttagcgtt agttttggtc acttagataa 82500 ttttagtgct aataattata aaaatgggtt agtggcgttg tattcactac caaaaacagg 82560 gtggatagaa tcaaagagta atggggttaa agtttttaaa tatgaatgta cggattattc 82620 tttagagatt aattacaatc cagaaagaag tagttttatg atttttaagg aaatttagtt 82680 ttatgttctt aagcacccta gggtgctttt ttaatgtctg atttttctga aacagtaatg 82740 gtgtaacctt tcatacggct agctaactcc atcattagag tttcggtagg gatcagttct 82800 acagtttttt cataattttg attttcaaag cttttttcaa gacgggcaac aatgtcggca 82860 ttcattgatc gactgtttaa ctttgctgat tcaagtattt tttcttttag ttcttgcgtc 82920 atacgcattt tgtattcaac gtctgagctt ctagccatgg tcctatactc gaataaattt 82980 tatttataat aatatcccca atggggattg acaagaagtt tttaaagtct taaattgtaa 83040 aagtccccat tggggatgta aaaagccccc aactttctga cggcaagggg cttttatcaa 83100 caaccatagg aaaggatatt gatatgtcta gtttagcatt aagttttaat gaagtgaaat 83160 tcaatcccgt gccacggcaa gatggccaga tttggctttc ttcaggtgaa ttggcacaag 83220 cattaggata taaacaagag aacgcggtca gtaaaatttt taatcgtaat tctgatgaat 83280 ttacggaaaa tatgacacaa attattgata atcctcggct acccaatttg ggtatgcgga 83340 tcttctcact acgtggctgc cacctaatag caatatttgc tcgtactgct gtagcgaagc 83400 aattccgcaa gtgggtactt gatgttttag ataaagaagt tggcacacca gttgccaaaa 83460 cccacaaatc cgaacgtgaa cccctaacca atgctgtaaa tcttcttgta gctaaaacta 83520 agcatttgaa ttacagcgat gcttataaat tagttcatca gcgtttcaat gttcagcata 83580 ttgatgaaat cccatatgac atgattcctg ttgcagtgga atatgttcat catctgattg 83640 cgatgtacag tagtgcagag aagaaggctc aaggttcttt atttgataat gaaacattgg 83700 gtttggttaa ggatctggta gatgcaatta tttcccaaaa ctttgtgaca agcaaaatct 83760 atcgtgcaat acacatgctt agtaatgaac aaggtcacta cttagctgaa tatgcgttta 83820 aaaccaatat tgcagttcta aaactcactc gaacaatgga tttaagagga cctcttaata 83880 gagaaatcat tagtgatgat ttaaaaacca taagctacac aacaggtaat caacattatg 83940 gcgaccgttg gtttcaccca ctgatggagt caagtcgatt gatgggagta cttgaaattt 84000 caggtagtct gattcgtcac taataaaatc aacttaacaa aacccactca tcgagtgggt 84060 tttttaatac ccaaaacaaa accccagtag cgctaactac cggggttttt cattccaccc 84120 accgacgaaa gtaagaggaa agtaaatcta tatggagcat tttaaaccaa tagtggagct 84180 tataaaagtg tctattgaaa agtatggctt atggcaaaca atagttgcat ttattctttt 84240 gttttccgtg ccaatcttaa tgtggaagtt ggatgtaatt attgcttcta taaaagcatg 84300 aaccaacttg aaaaaactgc gccaccttcg ggtggctttt ttacgtctaa aggaaagtga 84360 aatgaacatc gaacaatatc ttgatgagtt gatcaaacga gaaggcgggt acgtaaataa 84420 cccagcagac cgtggtggtg caactaagta tggaattact gaagcagttg ctcgagcaaa 84480 tggattcaaa ggtaatatgc gagatttacc tctggatgtg gccaaagcaa tttaccgcaa 84540 aaactattgg acagctccgc gatttgacca agtaaataca atcagctcag cagtggccga 84600 agagcttcta gacactggtg tgaattgcgg taccggcttt gcaaaacctc ttttacaacg 84660 agctttgaat ctcctaaata acaatggtaa agcagggtgg ccagatttat cagtagatgg 84720 gatatatggt ccggcaactc ttaatgcact caaaacttat ttggtcaaac gcgggaaaga 84780 aggagaaaaa gttttagttc gagttctgaa tattatgcaa ggtcagcgtt acattgaaat 84840 ctgtgagcgc aatccaagcc aagaacaatt tttctatggc tggattgcta atcgagtatc 84900 aatgtgaagt acctaatttt actgtgcatt ctactcaaga ctgcacagtt acttcgacgt 84960 atagtgaggt agttgtaaaa gtttataggt aagttatagg attgattggt aataatcttt 85020 aaattttagg gggggattgt tcagatgtag tgtattctgt aaaataaaac ttaattatat 85080 tttgctttca atacaatgaa cgatcaagtt ttccaattac aaattgttat aaatggaggt 85140 ttaaccccca ttcaatctaa gccagaaaca cttgataaat tagtaaaaga atttgctata 85200 aatcatttgt tgtttccaaa agaaataact gaacaattaa ttgagattaa ttctcaggat 85260 ggttctcaaa caaaaaagat aactaaattt attgatttgg ttagtagcaa tcaaaaatgt 85320 acctaccaaa ttagaaatga ttcacttgta tttttaaatt cctttgagaa aatagaggaa 85380 ttagaaagta tatttgaaaa gttttttaaa tcgttttcgg atctgacccc atatataaac 85440 tataaacaat caaaaagatt ggggctagtt cttattaggg aggattataa tgaggttaca 85500 ttacgcgagt attgtacttc agaagaatta gatcgaaatg ttattgagaa tagatcgaga 85560 aaagttactc gttttgctat ggcggaacta aatgaaatgg taaatttatc tgtttcaaaa 85620 gattatgtaa ctcatgaatc aggagtttct cgaaatactc tagcaagtgt ttatgatgtt 85680 aatacgttat ctactaagga tgtttttaga tttacaagta aggatgtagt aaagtttata 85740 aatgcttcaa agaaatttat tttagaatca atgtaagatt ttttatatga actattcaaa 85800 taatttatct aataatacta tgtatgaaag tggtaatcat aaaaagataa tcactaagta 85860 cgagattgaa acaataaaaa cggtttggca aaccgataag atacagtctt ttttaaaaga 85920 ttacttaaat acagacgtag ttagtattac tgaccccact gttatagaac aaaagatagg 85980 ggaagagagc ttaaagcaaa ttaaaagaga atttgatatt tttaagaata aatttgataa 86040 ttttctgaga tatgaagatg tgccagtcga ttatgtttca cctattgaaa atgaattaat 86100 caacttttat aaacatagta aagttgaagt tcaagaacaa attagccaat ggattattga 86160 ttcttttgat aacacaaagg ttcttcttaa tattttaaaa attttaggta atattgctcc 86220 tgattttatt gatcatcaat ttttaactaa ttttcttatc gttcttaatc ataaagatac 86280 tgaaatcaaa gaatatgcat taagaattca agagaaatta atgcttccat catataataa 86340 tgtactgaag cactctaagt taactccaaa atggattgat gactatagaa aagaattggt 86400 tgaattgtat gaagaagata ataaaggtag ttaatttaat atgactattt ttgtaagaaa 86460 gataagtaaa gcaaaatggc cttctgaaga ggaaattgca gaaaaagcac tggattcaga 86520 aattatacct tttgtcagag ccgatgcctt aactacttgt ttaaaaactt ctcaaaatac 86580 tttatctgtt tgggcagaaa gattaaaaga aataaaggcg gtttaaccgc cttaaactgt 86640 tgcaagctgt gctgtattaa gcacagtctt gctttgctca tatttcaaaa cgtctttctt 86700 tttatatgaa acacgtctcc caattttcga gaaaggcagt gatgattgat cacaacgcat 86760 tctagctaat gtccaaggcg agcaatctaa ataaagtgct acaacctctt gaggaaactt 86820 ctgttcttca ttagccatta tgaagcgatc caaatattct tgttgctctg catcagatag 86880 atttctcaga tcttttaaca tttactcctc cttactttcc gctttaactt ctcgatcaag 86940 acgctcaatc tccgcaatta ataaagcggc tgcacgaacc aaatcttgtc ttgggctttt 87000 tggtttccag aaagtatcag caaaaggcca ccatcctgca gcatcctcat ccatatacac 87060 ttcggggccg aaatctttgc tgttataaac ccaacctcta cttactacat gatcaacgta 87120 acaagatgca gctcgtggca attcatttgc ttcatagaga ttatcaaatt caggtaaata 87180 cccttctttt ataatttggc gttctctttc attaagaaca tctttgacag cttgactgct 87 240 atataattca ctcatccctc agctcccgat tcgcttgcca cttcaaccat caaagaataa 87300 ataggcgtgt aatgatcacg agtcccatcg ccccaatgga atccactatt ttctaaacag 87360 ctaacaacgc tgtctggaat ctctttcggt accaaacagt aaccctctgg caccgcctga 87420 gctttggctt tttctagctc tgcatcacga tgctttgcac atctaagcca agcatcccaa 87480 cgtctattca tgttgcttat ttctttctga gcaatttcag aaggattgtt tgatctagtc 87540 ataaacagtt catgctcatt actaaaaata atgtctcttc ttcctttgta atattggaag 87600 gtattcagaa aagcctctct ttccttattc aaatcaaaca tcattaggcc ctcaaatatt 87660 cttctttagt ccactcaaca aactctttat aaagctgctg cgcgggttta tttaaccggt 87720 tgtgatagtc gatcgttatg cggcgccaag cgactggtac cgcataatgt ttggttagaa 87780 acattgcttg atccatgcct tgccggacta ttacgtagcc cagcaattgc aagtagtaca 87840 taaaaccaag catgtgtttt tggctcactt tcttgtactg atctttcatg ttagaaaccg 87900 tccactaata aataatcagg gggagattct tgttgagtag gtgtaggatt ctctaat 87957
Claims (12)
Bacteriophage AB63 consisting of the nucleotide sequence represented by SEQ ID NO: 1, having a specific killing ability against Acinetobacter baumannii .
According to claim 1, wherein the bacteriophage is accession number KFCC11781P, bacteriophage AB63.
The bacteriophage AB63 according to claim 1, characterized in that the acinetobacter baumani bacterium is a bacterium antibiotic-resistant acinetobacter baumani bacterium.
The bacteriophage AB63 according to claim 3, characterized in that the carbapenem-based antibiotic is imipenem.
The bacteriophage AB63 according to claim 1, wherein the bacteriophage AB63 is stable in the range of pH3 to pH11.
The bacteriophage AB63 according to claim 1, wherein the bacteriophage AB63 is stable in the range of 40°C to 60°C.
A composition for antibiotics, comprising the bacteriophage AB63 of claim 1.
A composition for adding feed, comprising the bacteriophage AB63 of claim 1.
A feed comprising the composition for adding feed of claim 8.
A disinfectant comprising the bacteriophage AB63 of claim 1.
A cleaning agent comprising the bacteriophage AB63 of claim 1.
A method of preventing or treating an infectious disease caused by Acinetobacter baumani bacteria, comprising administering the bacteriophage AB63 of claim 1 to an individual other than a human.
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| WO2022065969A1 (en) * | 2020-09-28 | 2022-03-31 | 경북대학교 산학협력단 | Bacteriophage having potential of killing acinetobacter baumannii and composition comprising same |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| KR20130129326A (en) * | 2012-05-18 | 2013-11-28 | 연세대학교 산학협력단 | Lytic bacteriophage specific for acinetobacter genus resistant to carbarpenem |
| JP2015154771A (en) * | 2009-02-06 | 2015-08-27 | テクノファージ， インベスティガサン エ デセンボルビメント エム ビオテクノロジア，エスエー | Antibacterial phages, phage peptides and use methods thereof |
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| JP2015154771A (en) * | 2009-02-06 | 2015-08-27 | テクノファージ， インベスティガサン エ デセンボルビメント エム ビオテクノロジア，エスエー | Antibacterial phages, phage peptides and use methods thereof |
| KR20130129326A (en) * | 2012-05-18 | 2013-11-28 | 연세대학교 산학협력단 | Lytic bacteriophage specific for acinetobacter genus resistant to carbarpenem |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022065969A1 (en) * | 2020-09-28 | 2022-03-31 | 경북대학교 산학협력단 | Bacteriophage having potential of killing acinetobacter baumannii and composition comprising same |
| KR20220042688A (en) * | 2020-09-28 | 2022-04-05 | 경북대학교 산학협력단 | Bacteriophage having the ability to kill Acinetobacter baumannii and composition comprising the same |
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