KR20210156450A - Method for culturing watermelon scion and rootstock in plant factory using artificial lights - Google Patents

Abstract

The present invention relates to a method for cultivating a watermelon scion and rootstock in a plant factory using artificial lights, and provides a method for cultivating a scion and rootstock in a plant factory, which can provide a uniform watermelon scion and rootstock, allow the watermelon scion and rootstock to be used in an automatic grafting device such as a grafting robot to increase grafting production efficiency, and allow a cultivation environment for stable yearly production of standard seedlings of scions and rootstocks to be stably maintained throughout the year.

Description

Plant factory seedling system watermelon reception and rootstock cultivation method {Method for culturing watermelon scion and rootstock in plant factory using artificial lights}

The present invention relates to a plant factory seedling system watermelon receiving and culturing method of stock.

A method of producing grafted seedlings by attaching a plant's eye or eye-attached stem (scion) to a rooted stem or root (rootstock) is called grafting, and such grafting The seedlings produced through this process are called grafted seedlings.

The grafted rootstock and the stem are adhered to each other by the papillary tissue formed in the cambium at the connecting part, and the vascular genus are connected to grow as a complete plant.

Cutting is a vegetative propagation method in which a part of a plant's nutrient organs is separated from the parent body and inserted into soil or sand to root and germinate into an independent plant. There are types such as

In recent years, with the increase in the number of crops in the same arable land, continuous cultivation problems such as salt accumulation and the occurrence of soil diseases are emerging as serious problems. Even when growing fruits and vegetables such as peppers, tomatoes, and cucumbers, the occurrence of soil diseases such as late blight and blight is increasing as the number of crops increases and continuous cropping disturbances due to long-term cultivation.

The grafting cultivation method is a cultivation method that grafts a rootstock and a rootstock, and can be used as a cultivation method for fruits and vegetables including gourds and vegetables such as cucumbers and watermelons, and eggplant vegetables such as peppers and tomatoes.

In fact, in the case of fruits and vegetables such as watermelon, cucumber, and tomato, the use rate of grafted seedlings produced in process nurseries reaches about 70 to 90%, and a significant portion of them is produced by grafting cultivation method.

This grafting cultivation method has a great influence on the survival and acclimatization of grafted seedlings depending on the grafting degree of the rootstock and the seedling. In other words, precise grafting of rootstock and seedlings can greatly influence the improvement of crop productivity. Accordingly, grafting cultivation is carried out by hand as a whole, and skilled technical personnel are required. However, it is having difficulties in popularizing it due to the continuous decline of the rural population and the aging society.

Accordingly, Korean Patent Registration 10-0976555 (Application No. 10-2008-0025571, Applicant of the Republic of Korea Rural Development Administration) discloses a grafting robot for reducing the labor force at the seedling site and stably supplying and supplying grafted seedlings. No. 10-2016-0089040, Applicant Republic of Korea (Director of Rural Development Administration)) discloses a grafting robot and its operation method. Through these grafting robots, it became possible to automate grafting cultivation and through this, high-efficiency and high-reliability grafting cultivation.

However, the reception and rootstock used in these grafting robots have various shapes and sizes, and there has been a limit to the use of the grafting robot due to the characteristic that the uniformity is not high. Moreover, there is a problem in that it is difficult to produce standard seedlings stably throughout the year because the difficulty in the production of standard seedlings is increasing due to the high temperature environment in summer and lack of sunlight in winter due to climate change.

Accordingly, the inventors of the present invention have been studying a method for growing seedlings and seedlings in a plant factory in which the cultivation environment can be stably maintained throughout the year for the production of standard seedlings of seedlings and seedlings that are stable throughout the year. of the cultivation method was studied, and the present invention was completed.

One object of the present invention is (a) germinating watermelon seedlings and (b) the germinated watermelon seedlings under daytime temperature conditions of 27 to 25 ℃ and night temperature conditions of 16 to 20 ℃ and 50 to 250 μmol m ^{It is} to provide a cultivation method of watermelon reception comprising the step of culturing under light conditions of -2 s ^-1.

Another object of the present invention is (a) germinating watermelon stock seeds and (b) the germinated watermelon stock seeds in the daytime temperature condition of 27 to 25 ℃ and night temperature conditions of 16 to 20 ℃ and 50 to 250 μmol It is to provide a method for cultivating a watermelon stock comprising the step of culturing in a light intensity condition of ^{m -2} s ^-1.

One aspect of the present invention is (a) germinating the watermelon receiving seed and (b) the germinated watermelon receiving seed under daytime temperature conditions of 27 to 25 ℃ and night temperature conditions of 16 to 20 ℃ and 50 to 250 μmol m ^{- It} provides a cultivation method of watermelon reception comprising the step of cultivating in light conditions of 2 s ^-1.

The term “watermelon” used in the present invention is an annual plant of the dicotyledonous gourd family, which has the scientific name of Citrullus vulgaris. Watermelons have spherical fruit types: Taegeuk (early spherical species), Saetbyeol (semi-tactile regenerating species), Mt. Baekdu (large-fruited open-field medium cultivars), Hwanhoseong (large fruit type semi-contacting medium-sized species), and oval-shaped small species with oval shaped giraffe (medium) and medium-sized species. · Late species), etc., and in many cases it is cultivated by grafting it with the same or other species for the purpose of avoiding soil diseases and pests and promoting growth.

The step (a) is a step of germinating the watermelon receiving seed. The watermelon receiving seed may be made by a known method, for example, planting the watermelon receiving seed in a plug tray and filling it with a growth medium (artificial medium for seedling) Watermelon reception seeds can be planted with this method, and may be different from the rootstock seeds used for the method of growing watermelon stocks to be described later.

The step (b) is a step of cultivating the germinated watermelon receiving seed under a daytime temperature condition of 27 to 25°C, a night temperature condition of 16 to 20°C, and a light intensity condition of ^{50 to 250μmol m -2} s ^-1. The temperature condition of 27 to 25° C. corresponds to the daytime temperature condition, that is, the temperature condition during the light irradiation period. And the temperature condition of 16 to 20 ℃ corresponds to the night temperature condition, that is, the temperature condition in the dark period. The luminous intensity condition of 50 to 250 μmol m ^-2 s ^-1 corresponds to the daytime condition in natural cultivation as the luminous intensity condition during the light irradiation period. In step (b), specifically, the germinated watermelon receiving seed may be a daytime temperature condition of 26 to 25°C, a night temperature condition of 18 to 20°C, and a light intensity condition of ^{100 to 200μmol m -2} s ^{-1, more} Specifically, it may be cultivated under a day/night temperature condition of 25/20° C. and ^{a light intensity condition of 150 μmol m -2} s ^-1. When watermelon seed is grown under conditions outside of the above temperature and luminance conditions, watermelon seedlings not only overgrow or undergrow, but also receive watermelons of uniform quality enough to increase or utilize the production efficiency of the automatic grafting device. is not cultivated

In the step (b), in addition to the temperature condition and light intensity condition, the temperature and light intensity of the day temperature condition for 16 hours and the temperature of the night temperature condition for 8 hours are performed under 70% RH and 85% under night conditions under daylight conditions and daytime conditions. It may further include a humidity condition of RH.

The watermelon receiver grown by the cultivation method of the watermelon receiver may have a length of 5.4 to 10.4 cm, and a stem diameter of 1.55 to 1.82 mm. Specifically, the watermelon receiver may have a uniform size with a length of 7.1 cm and a stem diameter of 1.79 mm (Example 1).

As described above, the method for cultivating a watermelon receiver of the present invention is effective in providing watermelon receivers of uniform quality within the range of the length and stem diameter of the watermelon receiver.

And, the watermelon receiver cultivated by the cultivation method of the watermelon receiver may be for an automatic grafting device, and specifically may be for an automatic grafting robot. The watermelon seedlings grown by the above cultivation method are grown under the above-described temperature and light conditions to have a uniform quality within the range of the length and stem diameter of the watermelon base, rather than providing optimal watermelon seedlings for natural cultivation. In addition to having the effect of increasing the production efficiency of the grafting device, it is possible to provide a method of growing a plant in a plant factory in which the growing environment can be maintained stably throughout the year for the production of standard seedlings that are stable throughout the year.

Another aspect of the present invention is (a) germinating the watermelon stock seed and (b) the germinated watermelon stock seed under daytime temperature conditions of 27 to 25 ℃ and night temperature conditions of 16 to 20 ℃ and 50 to 250 μmol m ^It provides a method of cultivating a watermelon stock, comprising the step of culturing under a light intensity condition of -2 s ^-1.

The step (a) is a step of germinating watermelon stock seeds, and the watermelon stock seeds may be formed by a known method, for example, planting watermelon stock seeds in a plug tray and filling with a growth medium (artificial medium for seedlings). Watermelon root seeds can be planted with this method, and it may be different from the seedling seeds used in the above-described method for growing watermelon seedlings.

The step (b) is a step of cultivating the germinated watermelon stock seed under a daytime temperature condition of 27 to 25° C., a night temperature condition of 16 to 20° C., and a light intensity condition of ^{50 to 250 μmol m −2} s ^{−1 .} The temperature condition of 27 to 25° C. corresponds to the daytime temperature condition, that is, the temperature condition during the light irradiation period. And the night temperature condition of 16 to 20 ℃ corresponds to the temperature condition of the dark period. The luminous intensity condition of 50 to 250 μmol m ^-2 s ^-1 corresponds to the daytime condition in natural cultivation as the luminous intensity condition during the light irradiation period. Specifically, in step (b), the watermelon stock seed may be a daytime temperature condition of 26 to 25° C., a night temperature condition of 18 to 20, and a luminous intensity condition of ^{100 to 200 μmol m -2} s ^{-1, more specifically} It may be cultivated in a day/night temperature condition of 25/20° C. and ^{a light intensity condition of 150 μmol m -2} s ^-1. When the watermelon stock seed is grown under conditions outside the above temperature condition range and luminance condition range, watermelon stock not only overgrowth or undergrowth, but also watermelon stocks of uniform quality enough to increase or utilize the production efficiency of the automatic grafting device. It is not cultivated.

In the step (b), in addition to the temperature condition and light intensity condition, the temperature and light intensity of the day temperature condition for 16 hours and the temperature of the night temperature condition for 8 hours are performed under 70% RH and 85% under night conditions under daylight conditions and daytime conditions. It may further include a humidity condition of RH.

The watermelon stock grown by the cultivation method of the watermelon stock may have a length of 4.1 to 9.2 cm and a stem diameter of 2.01 to 2.47 mm. Specifically, the watermelon stock may have a uniform size with a length of 5.2 cm and a stem diameter of 2.40 mm (Example 2).

As described above, the method for cultivating a watermelon stock of the present invention is effective in providing a watermelon stock of uniform quality within the range of the length and stem diameter of the watermelon stock.

And, the watermelon stock grown by the cultivation method of the watermelon stock may be for an automatic grafting device, and specifically may be for an automatic grafting robot. The watermelon stock grown by the above cultivation method is grown under the above-mentioned temperature and light conditions to have a uniform quality within the range of the length and stem diameter of the watermelon stock, rather than providing optimal watermelon seedlings for natural cultivation. In addition to having the effect of increasing the production efficiency of the grafting device, it is possible to provide a method for growing stock in a plant factory in which the growing environment can be maintained stably throughout the year for the production of standard seedlings of stable stock throughout the year.

The cultivation method of the watermelon reception and the cultivation method of the watermelon root of the present invention can provide a uniform watermelon reception and rootstock, and the watermelon reception and the root can be utilized in an automatic grafting device such as a grafting robot to increase the grafting production efficiency. In addition, it is possible to provide a method for growing seedlings and stocks in a plant factory in which the cultivation environment can be maintained stably throughout the year for stable production of standard seedlings of seedlings and stocks throughout the year.

1 is a photograph confirming the reception of a watermelon grown under various temperature conditions and light intensity conditions according to Example 1.
FIG. 2 is a photograph confirming watermelon stocks grown under various temperature conditions and light intensity conditions according to Example 2. FIG.

Hereinafter, one or more specific examples will be described in more detail through examples. However, these examples are for illustrative purposes of one or more embodiments, and the scope of the present invention is not limited to these examples.

Example 1: Cultivation of watermelon reception

Plant the receiving seeds of the watermelon in a cell plug tray and fill with a growing medium (artificial medium for seedlings). This plug tray was placed in a germination chamber maintained at 28° C. for 2-3 days to germinate.

And, the germinated watermelon seedlings were subjected to a daytime temperature condition of 27 to 25 ℃, a night temperature condition of 16 to 20 ℃, and ^{a light intensity condition of 50 to 250 μmol m -2} s ^-1 , the temperature and light intensity of the daytime temperature condition for 16 hours and It was cultivated in a humidity condition of 70%RH under daylight conditions and daytime conditions, and 85%RH under night conditions, in which the temperature of the night temperature condition was performed for 8 hours.

As a result, as shown in FIG. 1 and Table 1, it was confirmed that the watermelon watermelon was cultivated in a uniform size with a length of 5.4 to 10.4 cm and a stem diameter of 1.55 to 1.82 mm. In addition, the watermelon stems grown under the temperature conditions of 25℃/20℃ (light irradiation period (daytime)/dark period (night)) and ^{luminous intensity conditions of 150μmol m -2} s ^-1 were 7.1 cm long and 1.79 mm in stem diameter. It was confirmed that it was the most suitable size for use in grafting, specifically, automatic grafting equipment, as it could provide a standard seedling reception of a stable size.

Temperature condition (℃)
(Day/Night) Luminance condition (mmol m ^-2 s ^-1 ) Length (cm) Stem Diameter (mm) 25/20 50 10.4 1.82 100 9.1 1.78 150 7.1 1.79 200 6.2 1.79 250 5.4 1.67 26/18 50 10.0 1.66 100 8.2 1.67 150 7.7 1.65 200 5.9 1.72 250 5.3 1.63 27/16 50 8.5 1.55 100 6.7 1.55 150 7.9 1.66 200 6.6 1.64 250 5.4 1.60

Example 2: Cultivation of watermelon stock

Watermelon stock seeds are planted in a plug tray and filled with growth medium (artificial medium for seedlings). This plug tray was placed in a germination chamber maintained at 28° C. for 2-3 days to germinate.

Then, the germinated watermelon stock seeds were subjected to a daytime temperature condition of 27 to 25 ℃ and a night temperature condition of 16 to 20 ℃ and ^{a light intensity condition of 50 to 250 μmol m -2} s ^-1 , the temperature and luminosity of the daytime condition for 16 hours and at night It was cultivated in a humidity condition of 70%RH under daylight conditions and daytime conditions, and 85%RH under night conditions in which the temperature and luminosity of the conditions were performed for 8 hours.

As a result, it was confirmed that the watermelon stock was grown in a uniform size with a length of 4.1 to 9.2 cm and a stem diameter of 2.01 to 2.47 mm as shown in FIGS. 2 and 2 . And, the watermelon root grown under the temperature condition of 25℃/20℃ (light irradiation period (daytime)/dark period (night)) and ^{the luminous intensity condition of 150μmol m -2} s ^-1 was 5.2cm long and 2.40mm in stem diameter. It was confirmed that it was the most suitable size for grafting, specifically, automatic grafting equipment, since it was possible to provide standard seedlings of a stable size.

Temperature condition (℃)
(Day/Night) Luminance condition (mmol m ^-2 s ^-1 ) Length (cm) Stem Diameter (mm) 25/20 50 7.1 2.30 100 5.2 2.37 150 5.2 2.40 200 5.2 2.47 250 4.5 2.44 26/18 50 9.2 2.47 100 6.1 2.14 150 5.8 2.26 200 4.6 2.18 250 5.7 2.29 27/16 50 5.0 2.08 100 4.1 2.01 150 6.0 2.49 200 5.3 2.35 250 5.2 2.22

So far, with respect to the present invention, the preferred embodiments have been looked at. Those of ordinary skill in the art to which the present invention pertains will understand that the present invention can be implemented in a modified form without departing from the essential characteristics of the present invention. Therefore, the disclosed embodiments are to be considered in an illustrative rather than a restrictive sense. The scope of the present invention is indicated in the claims rather than the foregoing description, and all differences within the scope equivalent thereto should be construed as being included in the present invention.

Claims (4)

(a) germinating the watermelon receiving seed; and
(b) cultivating the germinated watermelon seedlings under a daytime temperature condition of 27 to 25°C, a night temperature condition of 16 to 20°C, and a light intensity condition of ^{50 to 250μmol m -2} s ^-1;
Cultivation method of watermelon reception comprising a.
According to claim 1,
The cultivated watermelon reception is 5.4 to 10.4 cm in length, and the stem diameter is 1.55 to 1.82 mm. The cultivation method of the watermelon reception.
(a) germinating watermelon stock seeds; and
(b) cultivating the germinated watermelon stock seeds at a daytime temperature condition of 27 to 25°C, a night temperature condition of 16 to 20°C, and a light intensity condition of ^{50 to 250μmol m -2} s ^-1;
A method of growing a watermelon root comprising a.
4. The method of claim 3,
The cultivated watermelon stock has a length of 4.1 to 9.2cm, and a stem diameter of 2.01 to 2.47mm.

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