KR20210148188A - 유전자 사일런싱을 통한 식물 세포에서 해충 보호용 dsRNA 생산 - Google Patents
유전자 사일런싱을 통한 식물 세포에서 해충 보호용 dsRNA 생산 Download PDFInfo
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- KR20210148188A KR20210148188A KR1020217033295A KR20217033295A KR20210148188A KR 20210148188 A KR20210148188 A KR 20210148188A KR 1020217033295 A KR1020217033295 A KR 1020217033295A KR 20217033295 A KR20217033295 A KR 20217033295A KR 20210148188 A KR20210148188 A KR 20210148188A
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- rna
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- silencing
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- C—CHEMISTRY; METALLURGY
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/111—General methods applicable to biologically active non-coding nucleic acids
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8283—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for virus resistance
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8285—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for nematode resistance
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
- C12N2310/141—MicroRNAs, miRNAs
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
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- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- Insects & Arthropods (AREA)
- Pest Control & Pesticides (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB1903521.1A GB201903521D0 (en) | 2019-03-14 | 2019-03-14 | No title |
| GB1903521.1 | 2019-03-14 | ||
| PCT/IB2020/052245 WO2020183416A1 (en) | 2019-03-14 | 2020-03-12 | PRODUCTION OF dsRNA IN PLANT CELLS FOR PEST PROTECTION VIA GENE SILENCING |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| KR20210148188A true KR20210148188A (ko) | 2021-12-07 |
Family
ID=66381211
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020217033295A Ceased KR20210148188A (ko) | 2019-03-14 | 2020-03-12 | 유전자 사일런싱을 통한 식물 세포에서 해충 보호용 dsRNA 생산 |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US20220220494A1 (https=) |
| EP (1) | EP3938509A1 (https=) |
| JP (2) | JP2022524864A (https=) |
| KR (1) | KR20210148188A (https=) |
| CN (2) | CN113811612A (https=) |
| BR (1) | BR112021018120A2 (https=) |
| CA (1) | CA3132114A1 (https=) |
| GB (1) | GB201903521D0 (https=) |
| IL (1) | IL286381A (https=) |
| SG (1) | SG11202109507UA (https=) |
| WO (1) | WO2020183416A1 (https=) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SG11202002481RA (en) | 2017-09-19 | 2020-04-29 | Tropic Biosciences Uk Ltd | Modifying the specificity of non-coding rna molecules for silencing gene expression in eukaryotic cells |
| GB201903519D0 (en) * | 2019-03-14 | 2019-05-01 | Tropic Biosciences Uk Ltd | Introducing silencing activity to dysfunctional rna molecules and modifying their specificity against a gene of interest |
| GB202103256D0 (en) * | 2021-03-09 | 2021-04-21 | Tropic Biosciences Uk Ltd | Method for silencing genes |
| CN113699179B (zh) * | 2021-07-30 | 2022-04-05 | 广东省农业科学院植物保护研究所 | osa-miR-162a在制备驱避褐飞虱绿色农药中的应用 |
| US20250280834A1 (en) * | 2021-09-15 | 2025-09-11 | The University Of Queensland | Control of insect pests |
| CN116064644B (zh) * | 2022-10-10 | 2023-10-10 | 中国农业科学院生物技术研究所 | SbAGO1b蛋白及其编码基因在调控植物抗虫性中的应用 |
| CN115678903B (zh) * | 2022-11-03 | 2024-04-02 | 贵州大学 | 一种白背飞虱Ago1基因、合成dsRNA的方法及其应用 |
| CN116144681B (zh) * | 2022-12-29 | 2024-07-09 | 中国农业科学院植物保护研究所 | 番茄潜叶蛾Dnmt1基因及其在番茄潜叶蛾胁迫温度响应和发育中的作用 |
| CN116082481B (zh) * | 2023-03-15 | 2025-11-04 | 南京农业大学 | 一种影响植物对炭疽病敏感性的转录因子PbbZIP4、蛋白及其应用 |
| GB202305021D0 (en) | 2023-04-04 | 2023-05-17 | Tropic Biosciences Uk Ltd | Methods for generating breaks in a genome |
| CN116732041B (zh) * | 2023-05-23 | 2024-06-14 | 贵州大学 | 一种可用于rna干扰防治桃蚜的致死基因、rna干扰序列及其制备方法 |
| EP4497828A1 (en) * | 2023-07-28 | 2025-01-29 | Consejo Superior De Investigaciones Cientificas (Csic) | Minimal mirna precursors |
| CN120283056A (zh) * | 2023-10-30 | 2025-07-08 | 上海交通大学 | 一种靶向抑制基因表达的方法及应用 |
| CN117587018B (zh) * | 2023-12-22 | 2024-08-27 | 青岛农业大学 | 防止dsRNA菌液被紫外降解的方法及其在烟粉虱防治中的应用 |
| CN118726396B (zh) * | 2024-07-10 | 2025-07-29 | 西部(重庆)科学城种质创制大科学中心 | 过表达CiKPI基因及在提高柑橘炭疽病抗性的应用 |
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| CN120574876A (zh) | 2013-08-22 | 2025-09-02 | 纳幕尔杜邦公司 | 使用向导rna/cas内切核酸酶系统的植物基因组修饰及其使用方法 |
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2019
- 2019-03-14 GB GBGB1903521.1A patent/GB201903521D0/en not_active Ceased
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2020
- 2020-03-12 CN CN202080035482.8A patent/CN113811612A/zh active Pending
- 2020-03-12 CN CN202511990272.5A patent/CN121825969A/zh active Pending
- 2020-03-12 BR BR112021018120A patent/BR112021018120A2/pt unknown
- 2020-03-12 EP EP20715958.3A patent/EP3938509A1/en active Pending
- 2020-03-12 JP JP2021555366A patent/JP2022524864A/ja active Pending
- 2020-03-12 SG SG11202109507UA patent/SG11202109507UA/en unknown
- 2020-03-12 KR KR1020217033295A patent/KR20210148188A/ko not_active Ceased
- 2020-03-12 WO PCT/IB2020/052245 patent/WO2020183416A1/en not_active Ceased
- 2020-03-12 CA CA3132114A patent/CA3132114A1/en active Pending
- 2020-03-14 US US17/439,344 patent/US20220220494A1/en active Pending
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2021
- 2021-09-13 IL IL286381A patent/IL286381A/en unknown
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2025
- 2025-04-16 JP JP2025067723A patent/JP2025118671A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| JP2022524864A (ja) | 2022-05-10 |
| IL286381A (en) | 2021-10-31 |
| CN121825969A (zh) | 2026-04-10 |
| SG11202109507UA (en) | 2021-09-29 |
| AU2020236753A1 (en) | 2021-09-30 |
| GB201903521D0 (en) | 2019-05-01 |
| EP3938509A1 (en) | 2022-01-19 |
| BR112021018120A2 (pt) | 2021-11-16 |
| CN113811612A (zh) | 2021-12-17 |
| WO2020183416A1 (en) | 2020-09-17 |
| US20220220494A1 (en) | 2022-07-14 |
| JP2025118671A (ja) | 2025-08-13 |
| CA3132114A1 (en) | 2020-09-17 |
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