CN113811612A - 用于通过基因沉默的有害生物防治的数个植物细胞中的dsRNA的产生 - Google Patents

用于通过基因沉默的有害生物防治的数个植物细胞中的dsRNA的产生 Download PDF

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CN113811612A
CN113811612A CN202080035482.8A CN202080035482A CN113811612A CN 113811612 A CN113811612 A CN 113811612A CN 202080035482 A CN202080035482 A CN 202080035482A CN 113811612 A CN113811612 A CN 113811612A
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rna
plant
mir
gene
silencing
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艾尔·毛利
亚伦·盖伦提
克里斯蒂娜·皮诺奇
安吉拉·查帕罗·加西亚
奥菲尔·梅尔
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Tropic Biosciences UK Ltd
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Tropic Biosciences UK Ltd
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CN202080035482.8A 2019-03-14 2020-03-12 用于通过基因沉默的有害生物防治的数个植物细胞中的dsRNA的产生 Pending CN113811612A (zh)

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GBGB1903521.1A GB201903521D0 (en) 2019-03-14 2019-03-14 No title
GB1903521.1 2019-03-14
PCT/IB2020/052245 WO2020183416A1 (en) 2019-03-14 2020-03-12 PRODUCTION OF dsRNA IN PLANT CELLS FOR PEST PROTECTION VIA GENE SILENCING

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CN202511990272.5A Pending CN121825969A (zh) 2019-03-14 2020-03-12 用于通过基因沉默的有害生物防治的数个植物细胞中的dsRNA的产生

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113906138A (zh) * 2019-03-14 2022-01-07 热带生物科学英国有限公司 将沉默活性引入多个功能性失调的rna分子并修饰其对一感兴趣的基因的特异性
CN115678903A (zh) * 2022-11-03 2023-02-03 贵州大学 一种白背飞虱Ago1基因、合成dsRNA的方法及其应用
CN116144681A (zh) * 2022-12-29 2023-05-23 长江大学 番茄潜叶蛾Dnmt1基因及其在番茄潜叶蛾胁迫温度响应和发育中的作用

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG11202002481RA (en) 2017-09-19 2020-04-29 Tropic Biosciences Uk Ltd Modifying the specificity of non-coding rna molecules for silencing gene expression in eukaryotic cells
GB202103256D0 (en) * 2021-03-09 2021-04-21 Tropic Biosciences Uk Ltd Method for silencing genes
CN113699179B (zh) * 2021-07-30 2022-04-05 广东省农业科学院植物保护研究所 osa-miR-162a在制备驱避褐飞虱绿色农药中的应用
US20250280834A1 (en) * 2021-09-15 2025-09-11 The University Of Queensland Control of insect pests
CN116064644B (zh) * 2022-10-10 2023-10-10 中国农业科学院生物技术研究所 SbAGO1b蛋白及其编码基因在调控植物抗虫性中的应用
CN116082481B (zh) * 2023-03-15 2025-11-04 南京农业大学 一种影响植物对炭疽病敏感性的转录因子PbbZIP4、蛋白及其应用
GB202305021D0 (en) 2023-04-04 2023-05-17 Tropic Biosciences Uk Ltd Methods for generating breaks in a genome
CN116732041B (zh) * 2023-05-23 2024-06-14 贵州大学 一种可用于rna干扰防治桃蚜的致死基因、rna干扰序列及其制备方法
EP4497828A1 (en) * 2023-07-28 2025-01-29 Consejo Superior De Investigaciones Cientificas (Csic) Minimal mirna precursors
CN120283056A (zh) * 2023-10-30 2025-07-08 上海交通大学 一种靶向抑制基因表达的方法及应用
CN117587018B (zh) * 2023-12-22 2024-08-27 青岛农业大学 防止dsRNA菌液被紫外降解的方法及其在烟粉虱防治中的应用
CN118726396B (zh) * 2024-07-10 2025-07-29 西部(重庆)科学城种质创制大科学中心 过表达CiKPI基因及在提高柑橘炭疽病抗性的应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009006453A2 (en) * 2007-06-29 2009-01-08 Boston Biomedical, Inc. Enabling the use of long dsrna for gene targeting in mammalian and other selected animal cells
WO2016100333A1 (en) * 2014-12-15 2016-06-23 Syngenta Participations Ag Pesticidal microrna carriers and use thereof

Family Cites Families (56)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL154600B (nl) 1971-02-10 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van specifiek bindende eiwitten en hun corresponderende bindbare stoffen.
NL154598B (nl) 1970-11-10 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van laagmoleculire verbindingen en van eiwitten die deze verbindingen specifiek kunnen binden, alsmede testverpakking.
NL154599B (nl) 1970-12-28 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van specifiek bindende eiwitten en hun corresponderende bindbare stoffen, alsmede testverpakking.
US3901654A (en) 1971-06-21 1975-08-26 Biological Developments Receptor assays of biologically active compounds employing biologically specific receptors
US3853987A (en) 1971-09-01 1974-12-10 W Dreyer Immunological reagent and radioimmuno assay
US3867517A (en) 1971-12-21 1975-02-18 Abbott Lab Direct radioimmunoassay for antigens and their antibodies
NL171930C (nl) 1972-05-11 1983-06-01 Akzo Nv Werkwijze voor het aantonen en bepalen van haptenen, alsmede testverpakkingen.
US3850578A (en) 1973-03-12 1974-11-26 H Mcconnell Process for assaying for biologically active molecules
US3935074A (en) 1973-12-17 1976-01-27 Syva Company Antibody steric hindrance immunoassay with two antibodies
US3996345A (en) 1974-08-12 1976-12-07 Syva Company Fluorescence quenching with immunological pairs in immunoassays
US4034074A (en) 1974-09-19 1977-07-05 The Board Of Trustees Of Leland Stanford Junior University Universal reagent 2-site immunoradiometric assay using labelled anti (IgG)
US3984533A (en) 1975-11-13 1976-10-05 General Electric Company Electrophoretic method of detecting antigen-antibody reaction
US4098876A (en) 1976-10-26 1978-07-04 Corning Glass Works Reverse sandwich immunoassay
US4879219A (en) 1980-09-19 1989-11-07 General Hospital Corporation Immunoassay utilizing monoclonal high affinity IgM antibodies
CA1192510A (en) 1981-05-27 1985-08-27 Lawrence E. Pelcher Rna plant virus vector or portion thereof, a method of construction thereof, and a method of producing a gene derived product therefrom
JPS6054684A (ja) 1983-09-05 1985-03-29 Teijin Ltd 新規dνa及びハイブリツドdνa
US5011771A (en) 1984-04-12 1991-04-30 The General Hospital Corporation Multiepitopic immunometric assay
US4666828A (en) 1984-08-15 1987-05-19 The General Hospital Corporation Test for Huntington's disease
US4945050A (en) 1984-11-13 1990-07-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
CA1288073C (en) 1985-03-07 1991-08-27 Paul G. Ahlquist Rna transformation vector
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4801531A (en) 1985-04-17 1989-01-31 Biotechnology Research Partners, Ltd. Apo AI/CIII genomic polymorphisms predictive of atherosclerosis
GB8608850D0 (en) 1986-04-11 1986-05-14 Diatech Ltd Packaging system
JPS6314693A (ja) 1986-07-04 1988-01-21 Sumitomo Chem Co Ltd 植物ウイルスrnaベクタ−
US5015580A (en) 1987-07-29 1991-05-14 Agracetus Particle-mediated transformation of soybean plants and lines
ATE87032T1 (de) 1986-12-05 1993-04-15 Ciba Geigy Ag Verbessertes verfahren zur transformation von pflanzlichen protoplasten.
DE3850683T2 (de) 1987-02-09 1994-10-27 Lubrizol Genetics Inc Hybrides RNS-Virus.
US5316931A (en) 1988-02-26 1994-05-31 Biosource Genetics Corp. Plant viral vectors having heterologous subgenomic promoters for systemic expression of foreign genes
US5614395A (en) 1988-03-08 1997-03-25 Ciba-Geigy Corporation Chemically regulatable and anti-pathogenic DNA sequences and uses thereof
US5416011A (en) 1988-07-22 1995-05-16 Monsanto Company Method for soybean transformation and regeneration
US5693507A (en) 1988-09-26 1997-12-02 Auburn University Genetic engineering of plant chloroplasts
US5272057A (en) 1988-10-14 1993-12-21 Georgetown University Method of detecting a predisposition to cancer by the use of restriction fragment length polymorphism of the gene for human poly (ADP-ribose) polymerase
US5302523A (en) 1989-06-21 1994-04-12 Zeneca Limited Transformation of plant cells
US7705215B1 (en) 1990-04-17 2010-04-27 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US5550318A (en) 1990-04-17 1996-08-27 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US5192659A (en) 1989-08-25 1993-03-09 Genetype Ag Intron sequence analysis method for detection of adjacent and remote locus alleles as haplotypes
WO1991010725A1 (en) 1990-01-22 1991-07-25 Dekalb Plant Genetics Fertile transgenic corn plants
US5484956A (en) 1990-01-22 1996-01-16 Dekalb Genetics Corporation Fertile transgenic Zea mays plant comprising heterologous DNA encoding Bacillus thuringiensis endotoxin
US6403865B1 (en) 1990-08-24 2002-06-11 Syngenta Investment Corp. Method of producing transgenic maize using direct transformation of commercially important genotypes
US5384253A (en) 1990-12-28 1995-01-24 Dekalb Genetics Corporation Genetic transformation of maize cells by electroporation of cells pretreated with pectin degrading enzymes
ATE398679T1 (de) 1992-07-07 2008-07-15 Japan Tobacco Inc Verfahren zur transformation einer monokotyledon pflanze
US5281521A (en) 1992-07-20 1994-01-25 The Trustees Of The University Of Pennsylvania Modified avidin-biotin technique
JP2952041B2 (ja) 1992-07-27 1999-09-20 パイオニア ハイ−ブレッド インターナショナル,インコーポレイテッド 培養ダイズ細胞のagrobacterium媒介形質転換の改良法
US5693512A (en) 1996-03-01 1997-12-02 The Ohio State Research Foundation Method for transforming plant tissue by sonication
US5981840A (en) 1997-01-24 1999-11-09 Pioneer Hi-Bred International, Inc. Methods for agrobacterium-mediated transformation
WO2000042207A2 (en) 1999-01-14 2000-07-20 Monsanto Technology Llc Soybean transformation method
ATE548459T1 (de) 2005-09-16 2012-03-15 Monsanto Technology Llc Verfahren zur genetischen kontrolle von insektenbefall bei pflanzen und zusammensetzungen
ES2582091T3 (es) 2005-10-18 2016-09-09 Precision Biosciences Meganucleasas diseñadas racionalmente con especificidad de secuencia y afinidad de unión a ADN alteradas
RU2485180C2 (ru) 2007-06-07 2013-06-20 Эгрикалча Энд Эгри-Фуд Кэнэда Способ трансфекции и трансдукции растительных клеток
EP2195438B1 (en) 2007-10-05 2013-01-23 Dow AgroSciences LLC Methods for transferring molecular substances into plant cells
EP2292176B1 (en) 2009-09-07 2019-01-09 Nobel Biocare Services AG Implantation set
WO2013126963A1 (en) 2012-02-29 2013-09-06 Benitec Biopharma Limited Pain treatment
KR20150103280A (ko) 2013-01-08 2015-09-09 베니텍 바이오파마 리미티드 연령-관련 황반 변성 치료
CN120574876A (zh) 2013-08-22 2025-09-02 纳幕尔杜邦公司 使用向导rna/cas内切核酸酶系统的植物基因组修饰及其使用方法
CA2969619A1 (en) 2014-12-03 2016-06-09 Agilent Technologies, Inc. Guide rna with chemical modifications
SG11202002481RA (en) 2017-09-19 2020-04-29 Tropic Biosciences Uk Ltd Modifying the specificity of non-coding rna molecules for silencing gene expression in eukaryotic cells

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009006453A2 (en) * 2007-06-29 2009-01-08 Boston Biomedical, Inc. Enabling the use of long dsrna for gene targeting in mammalian and other selected animal cells
WO2016100333A1 (en) * 2014-12-15 2016-06-23 Syngenta Participations Ag Pesticidal microrna carriers and use thereof

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
JIANPING ZHOU 等: "CRISPR-Cas9 Based Genome Editing Reveals NewInsights into MicroRNA Function and Regulation in Rice", 《FRONTIERS IN PLANT SCIENCE》 *
唐涛;刘雪源;邱立红;: "RNA干扰及其对昆虫抗药性相关基因的沉默研究", 棉花学报, no. 06, pages 618 - 619 *
陈南颖;何延华;薄新文;王新华;: "RNA干扰及其在寄生线虫研究中的应用", 中国畜牧兽医, no. 02 *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113906138A (zh) * 2019-03-14 2022-01-07 热带生物科学英国有限公司 将沉默活性引入多个功能性失调的rna分子并修饰其对一感兴趣的基因的特异性
CN115678903A (zh) * 2022-11-03 2023-02-03 贵州大学 一种白背飞虱Ago1基因、合成dsRNA的方法及其应用
CN115678903B (zh) * 2022-11-03 2024-04-02 贵州大学 一种白背飞虱Ago1基因、合成dsRNA的方法及其应用
CN116144681A (zh) * 2022-12-29 2023-05-23 长江大学 番茄潜叶蛾Dnmt1基因及其在番茄潜叶蛾胁迫温度响应和发育中的作用
CN116144681B (zh) * 2022-12-29 2024-07-09 中国农业科学院植物保护研究所 番茄潜叶蛾Dnmt1基因及其在番茄潜叶蛾胁迫温度响应和发育中的作用

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