CN113811612A - 用于通过基因沉默的有害生物防治的数个植物细胞中的dsRNA的产生 - Google Patents
用于通过基因沉默的有害生物防治的数个植物细胞中的dsRNA的产生 Download PDFInfo
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- CN113811612A CN113811612A CN202080035482.8A CN202080035482A CN113811612A CN 113811612 A CN113811612 A CN 113811612A CN 202080035482 A CN202080035482 A CN 202080035482A CN 113811612 A CN113811612 A CN 113811612A
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- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
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- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
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- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8283—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for virus resistance
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- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8285—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for nematode resistance
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
- C12N2310/141—MicroRNAs, miRNAs
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- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
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- Medicinal Chemistry (AREA)
- Insects & Arthropods (AREA)
- Pest Control & Pesticides (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
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Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202511990272.5A CN121825969A (zh) | 2019-03-14 | 2020-03-12 | 用于通过基因沉默的有害生物防治的数个植物细胞中的dsRNA的产生 |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB1903521.1A GB201903521D0 (en) | 2019-03-14 | 2019-03-14 | No title |
| GB1903521.1 | 2019-03-14 | ||
| PCT/IB2020/052245 WO2020183416A1 (en) | 2019-03-14 | 2020-03-12 | PRODUCTION OF dsRNA IN PLANT CELLS FOR PEST PROTECTION VIA GENE SILENCING |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202511990272.5A Division CN121825969A (zh) | 2019-03-14 | 2020-03-12 | 用于通过基因沉默的有害生物防治的数个植物细胞中的dsRNA的产生 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113811612A true CN113811612A (zh) | 2021-12-17 |
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Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202080035482.8A Pending CN113811612A (zh) | 2019-03-14 | 2020-03-12 | 用于通过基因沉默的有害生物防治的数个植物细胞中的dsRNA的产生 |
| CN202511990272.5A Pending CN121825969A (zh) | 2019-03-14 | 2020-03-12 | 用于通过基因沉默的有害生物防治的数个植物细胞中的dsRNA的产生 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202511990272.5A Pending CN121825969A (zh) | 2019-03-14 | 2020-03-12 | 用于通过基因沉默的有害生物防治的数个植物细胞中的dsRNA的产生 |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US20220220494A1 (https=) |
| EP (1) | EP3938509A1 (https=) |
| JP (2) | JP2022524864A (https=) |
| KR (1) | KR20210148188A (https=) |
| CN (2) | CN113811612A (https=) |
| BR (1) | BR112021018120A2 (https=) |
| CA (1) | CA3132114A1 (https=) |
| GB (1) | GB201903521D0 (https=) |
| IL (1) | IL286381A (https=) |
| SG (1) | SG11202109507UA (https=) |
| WO (1) | WO2020183416A1 (https=) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113906138A (zh) * | 2019-03-14 | 2022-01-07 | 热带生物科学英国有限公司 | 将沉默活性引入多个功能性失调的rna分子并修饰其对一感兴趣的基因的特异性 |
| CN115678903A (zh) * | 2022-11-03 | 2023-02-03 | 贵州大学 | 一种白背飞虱Ago1基因、合成dsRNA的方法及其应用 |
| CN116144681A (zh) * | 2022-12-29 | 2023-05-23 | 长江大学 | 番茄潜叶蛾Dnmt1基因及其在番茄潜叶蛾胁迫温度响应和发育中的作用 |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SG11202002481RA (en) | 2017-09-19 | 2020-04-29 | Tropic Biosciences Uk Ltd | Modifying the specificity of non-coding rna molecules for silencing gene expression in eukaryotic cells |
| GB202103256D0 (en) * | 2021-03-09 | 2021-04-21 | Tropic Biosciences Uk Ltd | Method for silencing genes |
| CN113699179B (zh) * | 2021-07-30 | 2022-04-05 | 广东省农业科学院植物保护研究所 | osa-miR-162a在制备驱避褐飞虱绿色农药中的应用 |
| US20250280834A1 (en) * | 2021-09-15 | 2025-09-11 | The University Of Queensland | Control of insect pests |
| CN116064644B (zh) * | 2022-10-10 | 2023-10-10 | 中国农业科学院生物技术研究所 | SbAGO1b蛋白及其编码基因在调控植物抗虫性中的应用 |
| CN116082481B (zh) * | 2023-03-15 | 2025-11-04 | 南京农业大学 | 一种影响植物对炭疽病敏感性的转录因子PbbZIP4、蛋白及其应用 |
| GB202305021D0 (en) | 2023-04-04 | 2023-05-17 | Tropic Biosciences Uk Ltd | Methods for generating breaks in a genome |
| CN116732041B (zh) * | 2023-05-23 | 2024-06-14 | 贵州大学 | 一种可用于rna干扰防治桃蚜的致死基因、rna干扰序列及其制备方法 |
| EP4497828A1 (en) * | 2023-07-28 | 2025-01-29 | Consejo Superior De Investigaciones Cientificas (Csic) | Minimal mirna precursors |
| CN120283056A (zh) * | 2023-10-30 | 2025-07-08 | 上海交通大学 | 一种靶向抑制基因表达的方法及应用 |
| CN117587018B (zh) * | 2023-12-22 | 2024-08-27 | 青岛农业大学 | 防止dsRNA菌液被紫外降解的方法及其在烟粉虱防治中的应用 |
| CN118726396B (zh) * | 2024-07-10 | 2025-07-29 | 西部(重庆)科学城种质创制大科学中心 | 过表达CiKPI基因及在提高柑橘炭疽病抗性的应用 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113906138A (zh) * | 2019-03-14 | 2022-01-07 | 热带生物科学英国有限公司 | 将沉默活性引入多个功能性失调的rna分子并修饰其对一感兴趣的基因的特异性 |
| CN115678903A (zh) * | 2022-11-03 | 2023-02-03 | 贵州大学 | 一种白背飞虱Ago1基因、合成dsRNA的方法及其应用 |
| CN115678903B (zh) * | 2022-11-03 | 2024-04-02 | 贵州大学 | 一种白背飞虱Ago1基因、合成dsRNA的方法及其应用 |
| CN116144681A (zh) * | 2022-12-29 | 2023-05-23 | 长江大学 | 番茄潜叶蛾Dnmt1基因及其在番茄潜叶蛾胁迫温度响应和发育中的作用 |
| CN116144681B (zh) * | 2022-12-29 | 2024-07-09 | 中国农业科学院植物保护研究所 | 番茄潜叶蛾Dnmt1基因及其在番茄潜叶蛾胁迫温度响应和发育中的作用 |
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| JP2022524864A (ja) | 2022-05-10 |
| IL286381A (en) | 2021-10-31 |
| CN121825969A (zh) | 2026-04-10 |
| SG11202109507UA (en) | 2021-09-29 |
| AU2020236753A1 (en) | 2021-09-30 |
| GB201903521D0 (en) | 2019-05-01 |
| EP3938509A1 (en) | 2022-01-19 |
| BR112021018120A2 (pt) | 2021-11-16 |
| KR20210148188A (ko) | 2021-12-07 |
| WO2020183416A1 (en) | 2020-09-17 |
| US20220220494A1 (en) | 2022-07-14 |
| JP2025118671A (ja) | 2025-08-13 |
| CA3132114A1 (en) | 2020-09-17 |
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