KR20210145915A - Cultivating method for crops with high mineral contents - Google Patents

Abstract

The present invention relates to a method for cultivating crops containing inorganic materials to efficiently produce crops containing a large amount of inorganic materials. According to the present invention, the method comprises a step of spraying an aqueous inorganic solution to crops competing a rooting period and spraying the same once each of an effective tillering period, a booting period, and a heading and flowering period. The aqueous inorganic solution is manufactured by including the following steps: separating plasma proteins from livestock blood; hydrolyzing the separated plasma proteins to prepare a plasma protein hydrolysate; ultra-filtering the prepared plasma protein hydrolysate so that the hydrolysate of 100 to 2,000 Daltons is 40 to 60%; and lyophilizing the ultra-filtered plasma protein hydrolysate, adding and dissolving the lyophilized plasma protein hydrolysate in distilled water, and adding a water-soluble inorganic material to the mixture to prepare an aqueous inorganic solution.

Description

Cultivation method of crops containing inorganic substances. {CULTIVATING METHOD FOR CROPS WITH HIGH MINERAL CONTENTS}

The present invention relates to a method for cultivating a crop containing inorganic substances, and more particularly, to a cultivation method capable of producing a crop containing a large amount of minerals by spraying an aqueous mineral solution during the cultivation process of the crop.

Recently, with the development of new varieties of rice and the development of cultivation technology, the annual rice production has been steadily maintained at more than 5 million tons, while the annual rice consumption per capita is rapidly decreasing as the dietary patterns are gradually westernized and instantiated. continues to grow

In original rice, most of the total nutrients are in the rice germ and inner hull (more than 95% of minerals, vitamins and micronutrients), so brown rice with the germ and inner hull intact is the best nutritionally, but brown rice is There are problems in that it is rough to eat, not good in appearance, and the fiber is poorly digested, so most commercial rice except brown rice is shaved off when milling the germ and inner hull, and is polished to a transparent white luster and sold.

Although white rice contains nutrients such as carbohydrates, proteins, minerals, and vitamins, it lacks fat, calcium, and iron, so immunity to various diseases caused by malnutrition is lowered. It is converted into an acidic constitution, which directly or indirectly causes adult diseases such as constipation, arteriosclerosis, and high blood pressure.

In addition, when the peel of a fruit or grain is peeled, various trace mineral elements such as potassium, zinc, boron, copper, and selenium, which prevent oxidation in the peel, are lost in polished rice as soon as oxidation occurs, that is, decay proceeds. After a long period of time, because oxidation has already progressed a lot, microscopic harmful mold is formed in the polished rice, which is inconvenient to wash with water right before cooking.

In order to compensate for these problems, recently, research on functional rice with increased taste and nutrition has been actively conducted.

For example, in the case of germanium rice, it provides sufficient oxygen to each organ of the human body to activate cell functions, facilitates metabolism, and normalizes the functions of each organ to maintain health. Also, harmful oxygen and hydrogen ions in the body Germanium rice is produced by grafting germanium, which is known to enhance immunity by removing cellular waste, smoothing cell functions, and activating immune cells of the human body. As a method of producing such germanium rice, a method of producing germanium rice by spraying inorganic germanium ore powder on rice fields has been tried. However, in the case of spraying germanium in the form of ore powder to the rice fields, there is a problem that the germanium absorption rate is low and the effect is inferior compared to the expensive production cost. There is a problem with throwing away.

On the other hand, the applicant, through Korean Patent No. 10-1687208, freeze-dried plasma protein hydrolyzate to produce germanium-containing eggs, dissolved by adding water-soluble organic germanium, We have developed a technology that allows a large amount of germanium to be contained in the produced eggs, and if this technology is applied, it is expected that various crops including rice containing a large amount of various minerals including germanium can be produced.

Republic of Korea Patent Publication No. 10-1687208 Republic of Korea Patent Publication No. 10-1315262 Republic of Korea Patent Publication No. 10-1261355

The present invention has been devised in view of the problems of the prior art as described above, and the purpose of the present invention is to provide a method for growing crops that can efficiently produce crops containing a large amount of minerals by spraying an aqueous mineral solution during the cultivation process of crops. do.

The cultivation method of the inorganic material-containing crop of the present invention for achieving the above object is a method of planting an aqueous solution of one or more inorganic materials selected from germanium, selenium, calcium, iron, vanadium, copper, nickel, zinc, magnesium, chromium, and gold in a paddy field. A method of growing a crop comprising spraying the rice that has gone through the ripening stage, and spraying it once each during the effective tillering period, the seeding period, and the seedling and flowering period, wherein the inorganic aqueous solution is a step of separating plasma proteins from livestock blood, the separated plasma Preparing a plasma protein hydrolyzate by hydrolyzing the protein, ultra-filtering the prepared plasma protein hydrolyzate so that the hydrolyzate of 100 to 2,000 Daltons is 40 to 60% Freezing the ultrafiltered plasma protein hydrolyzate It is characterized in that it is prepared including the step of dissolving by adding it to distilled water after drying, and adding a water-soluble inorganic raw material to prepare an inorganic aqueous solution.

At this time, the crops are rice, pine nuts, chestnuts, barley, wheat, corn, cucumber, eggplant, peas, green beans, perilla, sesame, goji berries, cherry, sorghum, grapes, raspberries, marigold, jujube, apple, pear, persimmon, plum , walnut, plum, peach, tomato, strawberry, melon, watermelon, melon, red pepper, green pepper, hazelnut, cucurbita, mulberry, dried crab, pomegranate, durian, mango, acai berry, granberry, raspberry, aronia, maki Berry, blackcurrant, elderberry, lingonberry, cranberry, bilberry, huckleberry, loganberry, snowberry, bearberry, papaya, avocado, grapefruit, lime, cherry, dragon fruit, cherry, banana, kiwi, coconut, pineapple, pomelo, Maca, Longan, Red Hyang, Cheon Hye Hyang, Hallabong, Tangerine, Eurem, Yeoju, Griviola, Aronia, Black locust, Brown flower, Schisandra, Yulmu, Spiny hornwort, Sancho, Cheonnyanggeum, Calamansi, Donga, Cloth tree, Peanut tree, Beetle It may be any one of chrysanthemum, serrata, oleifera, chrysanthemum, cornflower, vitamin tree, noni, fig tree, coffee tree, actinidia, and mulberry.

In addition, the inorganic aqueous solution may be additionally sprayed during the ripening period of crops.

The method for growing crops according to the present invention exhibits the effect of efficiently producing crops containing a large amount of minerals by spraying an aqueous mineral solution during the cultivation of rice.

1 is a result of measuring the germanium content of rice harvested according to an embodiment.
2 is a result of measuring the selenium content of rice harvested according to another embodiment.
3 is a result of measuring the germanium content of perilla, cucumber, melon, and grape grown according to another embodiment.

Hereinafter, the present invention will be described in more detail. The terms or words used in the present specification and claims should not be construed as being limited to their ordinary or dictionary meanings, and the inventor may properly define the concept of the term in order to best describe his invention. Based on the principle that there is, it should be interpreted as meaning and concept consistent with the technical idea of the present invention.

The cultivation method of a crop of the present invention is a crop grown by spraying an aqueous solution of one or more inorganic substances selected from germanium, selenium, calcium, iron, vanadium, copper, nickel, zinc, magnesium, chromium, and gold in the normal cultivation process of crops. It has a technical feature to contain a large amount of inorganic matter. The inorganic aqueous solution may be sprayed on crops that have undergone the rooting season, but may be sprayed once each during the effective tillering period, suing period, and outgoing flowering period. In addition, if necessary, the inorganic aqueous solution can be additionally sprayed during the ripening period, the period from flowering to completion of fertilization and the growth and hypertrophy of the ovaries to maturity. This can further increase the mineral content.

The crops to which the aqueous mineral solution of the present invention can be applied include rice, pine nuts, chestnuts, barley, wheat, corn, cucumber, eggplant, peas, green beans, perilla, sesame, goji berries, cherries, sorghum, grapes, raspberries, mulberry, Jujube, apple, pear, persimmon, plum, walnut, plum, peach, tomato, strawberry, melon, watermelon, melon, red pepper, green pepper, hazelnut, cucumber, mulberry, pomegranate, durian, mango, acai berry, Granberry, raspberry, aronia, maquiberry, blackcurrant, elderberry, lingonberry, cranberry, bilberry, huckleberry, loganberry, snowberry, bearberry, papaya, avocado, grapefruit, lime, cherry, dragon fruit, cherry, banana , Kiwi, Coconut, Pineapple, Pomelo, Maca, Longan, Red Hyang, Cheonhyehyang, Hallabong, Tangerine, Eureum, Yeoju, Griviola, Aronia, Black ragweed, Brown flower, Omija, Yulmu, Spiny cyst, Sancho, Cheonnyangeum, Calamansi, Donga, clothes tree, pheasant tree, bepung lantern, serrata tree, oleifera, jeongnara, cornus tree, vitamin tree, noni, fig tree, coffee tree, actinidia, and mulberry can be mentioned.

The inorganic aqueous solution is a peptide pool in which minerals are bound to a hydrolyzate of livestock plasma protein, and the applicant applied a peptide pool in which germanium is bound to a hydrolyzate of livestock plasma protein through Korean Patent Publication No. 10-1687208 to egg production, as well as bean sprouts. It has also been used in the cultivation method (Korean Patent Publication No. 10-1315262) and strawberry cultivation method (Korean Patent Publication No. 10-1261355), and is known as an effective method to contain minerals in crops.

The inorganic aqueous solution is prepared by separating plasma protein from livestock blood, hydrolyzing it, ultra-filtering it to 3,000 daltons or less, and then freeze-drying and dissolving it or obtaining a fraction. The reason for filtration to 3,000 daltons or less is It is also for the purpose of improving the absorption rate of inorganic substances, but it is also because ultrafiltration is employed in the established manufacturing process, and the molecular weight can be obtained with a conventional ultrafiltration membrane having a molecular weight limit of 5,000.

In the present invention, in order to increase the absorption efficiency of inorganic substances absorbed by crops when spraying in the process of growing crops, separating plasma proteins from livestock blood, hydrolyzing the separated plasma proteins to prepare a hydrolyzate of plasma proteins , ultra-filtering the prepared plasma protein hydrolyzate so that the hydrolyzate of 100 to 2,000 Daltons is 40 to 60%, freeze-drying the ultra-filtered plasma protein hydrolyzate, dissolve it in distilled water, and add a water-soluble inorganic material To prepare the inorganic aqueous solution, including the step of preparing the inorganic aqueous solution.

This will be described in detail as follows.

After slaughtering livestock such as cattle, pigs, chickens, geese, and ducks, the blood obtained from livestock is treated with a continuous centrifuge to separate plasma proteins, and then 2 to 10% trichloroacetic acid, 0.6 to 3 N hydrochloric acid, The plasma protein is precipitated with 50 to 70% ammonium sulfate. After centrifugation, the precipitated plasma proteins are heat-treated at 95°C for 20 minutes, and then hydrolyzed at 50°C and pH 8.2±0.2 for 9 hours by adding 2.5 wt% of a commercial proteolytic enzyme, flavourzyme. The degree of hydrolysis was measured by GPC in TNBS medium. By calculating the peak area of the molecular weight distribution of the hydrolyzate, it was found that 40 to 60% of molecular weights greater than or equal to 100 Daltons and less than or equal to 2,000 Daltons were obtained.

The hydrolyzate is subjected to primary ultrafiltration using an ultrafiltration membrane having a molecular weight limit of 5,000 and second ultrafiltration using an ultrafiltration membrane having a molecular weight limit of 1,000 to obtain an ultrafiltered plasma protein hydrolyzate.

The plasma protein hydrolyzate is loaded on gel filtration chromatography (Sephadex G15) and fractionated by flowing a phosphate buffer solution at a flow rate of 0.3 ml/min. After separating the F-2 (Fraction-2) fraction to obtain a fraction, it can be lyophilized. The reason for separating the F-2 fraction is that it shows the highest binding force with inorganic substances among the fractions. In addition, the ultra-filtered plasma protein hydrolyzate is freeze-dried without fractionation, and then the dried powder is added to distilled water to dissolve, and a water-soluble inorganic material is added to prepare an inorganic aqueous solution.

The F-2 fraction separated from the plasma protein hydrolyzate was freeze-dried and dissolved in distilled water at a concentration of 1 g/L, and in the case of germanium, water-soluble organic Ge (Ge-132) was added at a level of 2000 mg/L. This solution was stirred at room temperature for 1 hour to complete the reaction when the solution became transparent, and it was confirmed by using IPC-MS that 2,000 ppm of water-soluble peptide-germanium was formed. In addition to the germanium, a water-soluble organometallic compound may be used in the case of selenium, calcium, iron, vanadium, copper, nickel, zinc, magnesium, chromium, gold, and the like.

The inorganic aqueous solution prepared in this way is diluted to a predetermined concentration or less in distilled water, adjusted to a desired concentration, and then the process of spraying the crops is performed as described above. In the case of rice, the spraying is preferably side-side spraying, and the inorganic aqueous solution is sprayed according to the effective tillering period, which is the period from the beginning of tillering to the effective tillering period.

Next, from the time the young ear is differentiated to just before the ear is harvested, it goes through the elongation period (伸long期). The elongation stage is the period in which the length between the nodes at the base of the stem is elongated and completed, and it can be divided into a running water forming period and a watering period. The oil-watering period refers to the period from about 30 days after seeding, when the young ears start to differentiate, the shells of the rice grains are formed, and the ears grow to 3 to 5 cm. This is the period from 10 to 12 days before seeding to just before the ears are harvested, during which time the ears grow rapidly. Secondary spraying of the inorganic aqueous solution in the suinggi.

The ears go through the harvesting season, and in the case of rice, the ears are extracted from the branches to the outside and go through the seeding and flowering season, which is the period until the rice flowers bloom. Depending on the degree of sprouting of the ears, the flowering period is at the time of seeding (when about 20% of the total number of stems have sprouted), and the season of seeding (when about 40-50% of the number of stems have sprouted). It is divided into , sugijeon (穗jeon 期) (the period when 80-90% of the total number of stems are watered out). The inorganic aqueous solution is sprayed tertiarily once during the flowering period.

Next, it undergoes flowering and fertilization, and after fertilization, the endosperm tissue is rapidly formed inside the ovary of the pistil, and at the same time, carbohydrates start to move from the stem and leaves to the ear, and are accumulated as starch grains in the endosperm cells. The endosperm cell division and proliferation ends on the 9th day after flowering, and on the 15th day, the appearance resembles brown rice, and the storage of starch continues until the 35th day. Rice starch is a complex starch grain, in which many microscopic polygonal crystals are gathered to form a single grain. When the rice is in an immature state, when it is pressed, endosperm starch is released. This period is called the ripening period (乳熟期). If the starch increases further, the endosperm becomes more transparent from the center, and the rice becomes heavy and the ears droop downward. The time when the rice hulls turn yellow is called the yellow ripening period, and the time when the endosperm is completely enriched after more than 40 days after flowering is called the ripening period. Also, the embryo develops at the base of the endosperm, and by the 15th day after flowering, the vigor of young eyes or young roots is differentiated, and the form of rice is almost completed.

Usually, 40 to 45 days after the ear is harvested in August, the maturity period is in mid-to-late September, and functional rice containing inorganic substances according to the present invention can be harvested.

A test for producing germanium-containing rice and selenium-containing rice was conducted by applying the crop cultivation method according to the present invention. For the test, the rice cultivation method according to the present invention as described above was performed by selecting Chucheong rice as a rice variety.

It was sown on April 21, and transplanted to the main paddy field around May 23-25. About 90kg was put into the paddy field of about 8,000 pyeong, and other management was produced according to farmhouse practices.

In addition, the germanium aqueous solution and the selenium aqueous solution were diluted in water to a concentration of 26.7 mg/L and sprayed to the rice at a level of 45L/10a. Effective tillering season (August 4), which is the period from the beginning of tillering shoots to the effective tillering period, and the period in which the sheath of the uppermost branch bulges, the number of periods from 10 to 12 days before seeding to just before the ears are cut It was sprayed 4 times in accordance with the growing season (August 18), the period from when the ears of rice were extracted from the leaves to the outside of the leaf and before the rice flowers bloomed (August 27) and ripening (September 27). .

The spraying method was carried out as a side spraying method of rice using a high-pressure sprayer for a vehicle, and the germanium content and selenium content of the harvested rice were measured. As a result, as shown in FIG. 1 , the germanium content of brown rice was 11.9 μg/kg, and the germanium content was 7.9 μg/kg in the case of 7 minutes, confirming that brown rice had a higher germanium content than that of 7 minutes. In addition, as in FIG. 2 , the selenium content of brown rice was 12.1 μg/kg, and the selenium content was 8.1 μg/kg in the case of 7 minutes, confirming that brown rice had a higher selenium content than that of 7 minutes. It was judged that this was because the rice buds and inner hull were scraped off during the milling process at 7 minutes.

In addition, as a control, when the hydrolyzate of plasma protein was ultra-filtered with a molecular weight of 3,000 Dalton or less according to the prior art to obtain a fraction to prepare a water-soluble peptide-germanium and sprayed in the same manner, as in FIG. 1 , the germanium content in brown rice This is 8.2 μg/kg, and it was found that the germanium content was 2.8 μg/kg for 7 minutes, and in FIG. 2, the selenium content was 8.2 μg/kg for brown rice, and the selenium content was 2.9 μg/kg for 7 minutes. It was found that the content of minerals was relatively low. In particular, it was estimated that the sprayed inorganic component did not sufficiently penetrate to the inside of the rice because the difference between the germanium and selenium content of 7 minutes and brown rice was larger. These results suggest that the spraying of the inorganic aqueous solution according to the present invention can further increase the absorption power for rice.

Therefore, according to the present invention, by producing functional rice containing inorganic substances by the spraying method of an aqueous solution of inorganic substances in a dissolved form according to the present invention, not only the absorption rate of inorganic substances in rice is improved, but also the absorption rate of the human body is improved even when ingested by humans. As a result, functional rice can be produced by treating a small amount of inorganic matter, which has the advantage of high production efficiency at low cost. In addition, according to the present invention, the concentration of inorganic substances in the aqueous solution can be freely adjusted to a desired concentration, and accordingly, there is an advantage in that even in harvested rice, customized rice having a predetermined concentration can be produced.

In addition, the germanium aqueous solution was sprayed with a side spraying method at a concentration of 26.7 mg/L using a high-pressure sprayer for a vehicle during the effective tillering period, suing period, and flowering period during the cultivation of perilla, cucumber, melon, and grapes (experimental group). The germanium content was compared with the untreated group that did not spray the inorganic aqueous solution, and in order to check the difference in the germanium content according to the type of the germanium aqueous solution, 100 to 2,000 daltons of hydrolyzate in the plasma protein hydrolyzate when preparing the germanium aqueous solution was 20 to The germanium aqueous solution prepared by ultrafiltration to 30% was sprayed in the same way (comparative group).

As a result, it was confirmed that a large amount of germanium content was exhibited in the cultivated crops when the inorganic aqueous solution according to the present invention was sprayed as shown in FIG. 3 . In addition, as compared to the comparative group, the germanium content was significantly increased in the experimental group, so it was confirmed that the inorganic substances contained in the crops could be increased when the inorganic aqueous solution according to the present invention was applied.

The present invention has been described with reference to a preferred embodiment as described above, but it is not limited to the above embodiment, and various modifications and variations by those skilled in the art to which the present invention pertains within the scope not departing from the spirit of the present invention may be made. Changes are possible. Such modifications and variations are intended to fall within the scope of the present invention and the appended claims.

Claims (3)

An aqueous solution of one or more inorganic substances selected from germanium, selenium, calcium, iron, vanadium, copper, nickel, zinc, magnesium, chromium, and gold is sprayed on the crops that have gone through the rooting period, but once each during the effective tillering period, seedling period and flowering period. As a method of growing crops comprising the step of spraying,
The inorganic aqueous solution is
Separating plasma proteins from livestock blood;
preparing a plasma protein hydrolyzate by hydrolyzing the separated plasma protein;
Ultra-filtering the prepared plasma protein hydrolyzate so that the hydrolyzate of 100 to 2,000 Daltons is 40 to 60%;
preparing an inorganic aqueous solution by lyophilizing the ultra-filtered plasma protein hydrolyzate, dissolving it in distilled water, and adding a water-soluble inorganic raw material;
A method of growing an inorganic material-containing crop, characterized in that it is prepared including a.
The method according to claim 1,
The above crops include rice, pine nuts, chestnuts, barley, wheat, corn, cucumber, eggplant, peas, green beans, perilla, sesame, wolfberry, cherry, sorghum, grapes, wild raspberry, marigold, jujube, apple, pear, persimmon, plum, walnut , plum, peach, tomato, strawberry, melon, watermelon, melon, red pepper, green pepper, hazelnut, mulberry, mulberry, pomegranate, durian, mango, acai berry, grandberry, raspberry, aronia, maquiberry, Blackcurrant, elderberry, lingonberry, cranberry, bilberry, huckleberry, loganberry, snowberry, bearberry, papaya, avocado, grapefruit, lime, cherry, dragon fruit, cherry, banana, kiwi, coconut, pineapple, pomelo, maca, Longan, Red Hyanghyang, Cheonhyehyang, Hallabong, Tangerine, Eureum, Yeoju, Griviola, Aronia, Blackberry, Galhwa, Schisandra, Yulmu, Spiny hornwort, Sancho, Cheonnyanggeum, Calamansi, Donga, Cloth tree, Pheasant tree, Bepung, Cultivation method of mineral-containing crops, characterized in that any one of serrata, oleifera, jeongnara, cornflower, vitamin tree, noni, fig tree, coffee tree, actinidia, and mulberry.
The method according to claim 1,
A method of growing an inorganic material-containing crop, characterized in that the inorganic aqueous solution is additionally sprayed during the ripening period of the crop.

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