KR20000063783A - Manufacture of functional drink using bovine and porcine blood plasma protein hydrolysates - Google Patents
Manufacture of functional drink using bovine and porcine blood plasma protein hydrolysates Download PDFInfo
- Publication number
- KR20000063783A KR20000063783A KR1020000045023A KR20000045023A KR20000063783A KR 20000063783 A KR20000063783 A KR 20000063783A KR 1020000045023 A KR1020000045023 A KR 1020000045023A KR 20000045023 A KR20000045023 A KR 20000045023A KR 20000063783 A KR20000063783 A KR 20000063783A
- Authority
- KR
- South Korea
- Prior art keywords
- plasma protein
- blood
- blood plasma
- bovine
- preparation
- Prior art date
Links
- 102000004506 Blood Proteins Human genes 0.000 title claims abstract description 19
- 108010017384 Blood Proteins Proteins 0.000 title claims abstract description 19
- 210000002381 plasma Anatomy 0.000 title claims abstract description 6
- 239000003531 protein hydrolysate Substances 0.000 title claims description 14
- 241000283690 Bos taurus Species 0.000 title description 5
- 238000004519 manufacturing process Methods 0.000 title description 3
- 238000000034 method Methods 0.000 claims abstract description 10
- 235000000832 Ayote Nutrition 0.000 claims abstract description 9
- 235000009854 Cucurbita moschata Nutrition 0.000 claims abstract description 9
- 235000009804 Cucurbita pepo subsp pepo Nutrition 0.000 claims abstract description 9
- 235000015136 pumpkin Nutrition 0.000 claims abstract description 9
- 240000001980 Cucurbita pepo Species 0.000 claims abstract description 7
- 239000000284 extract Substances 0.000 claims abstract description 6
- 230000001954 sterilising effect Effects 0.000 claims abstract description 4
- 238000002360 preparation method Methods 0.000 claims abstract 3
- 230000007062 hydrolysis Effects 0.000 claims description 6
- 238000006460 hydrolysis reaction Methods 0.000 claims description 6
- 238000001914 filtration Methods 0.000 claims description 5
- 239000000796 flavoring agent Substances 0.000 claims description 5
- 235000003599 food sweetener Nutrition 0.000 claims description 5
- 239000003755 preservative agent Substances 0.000 claims description 5
- 239000003381 stabilizer Substances 0.000 claims description 5
- 239000003765 sweetening agent Substances 0.000 claims description 5
- -1 acidulants Substances 0.000 claims description 4
- 235000013355 food flavoring agent Nutrition 0.000 claims description 4
- 229930003231 vitamin Natural products 0.000 claims description 4
- 239000011782 vitamin Substances 0.000 claims description 4
- 235000013343 vitamin Nutrition 0.000 claims description 4
- 229940088594 vitamin Drugs 0.000 claims description 4
- 238000004659 sterilization and disinfection Methods 0.000 claims description 3
- 238000000108 ultra-filtration Methods 0.000 claims description 3
- 235000013361 beverage Nutrition 0.000 claims description 2
- 239000012528 membrane Substances 0.000 claims description 2
- 239000004615 ingredient Substances 0.000 claims 1
- 210000004369 blood Anatomy 0.000 abstract description 11
- 239000008280 blood Substances 0.000 abstract description 11
- 206010020772 Hypertension Diseases 0.000 abstract description 9
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 abstract description 9
- 201000010099 disease Diseases 0.000 abstract description 6
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 6
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Natural products OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 abstract description 4
- 229930091371 Fructose Natural products 0.000 abstract description 4
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 abstract description 4
- 239000007788 liquid Substances 0.000 abstract description 3
- 239000000463 material Substances 0.000 abstract description 3
- 238000002156 mixing Methods 0.000 abstract description 3
- 229950008882 polysorbate Drugs 0.000 abstract description 3
- 229920000136 polysorbate Polymers 0.000 abstract description 3
- WXMKPNITSTVMEF-UHFFFAOYSA-M sodium benzoate Chemical compound [Na+].[O-]C(=O)C1=CC=CC=C1 WXMKPNITSTVMEF-UHFFFAOYSA-M 0.000 abstract description 3
- AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 abstract description 2
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 abstract description 2
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 abstract description 2
- 102000005158 Subtilisins Human genes 0.000 abstract description 2
- 108010056079 Subtilisins Proteins 0.000 abstract description 2
- 229930003471 Vitamin B2 Natural products 0.000 abstract description 2
- 229930003268 Vitamin C Natural products 0.000 abstract description 2
- 239000008369 fruit flavor Substances 0.000 abstract description 2
- 229960002477 riboflavin Drugs 0.000 abstract description 2
- 235000019164 vitamin B2 Nutrition 0.000 abstract description 2
- 239000011716 vitamin B2 Substances 0.000 abstract description 2
- 235000019154 vitamin C Nutrition 0.000 abstract description 2
- 239000011718 vitamin C Substances 0.000 abstract description 2
- LXNHXLLTXMVWPM-UHFFFAOYSA-N pyridoxine Chemical compound CC1=NC=C(CO)C(CO)=C1O LXNHXLLTXMVWPM-UHFFFAOYSA-N 0.000 abstract 2
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- DFPAKSUCGFBDDF-UHFFFAOYSA-N Nicotinamide Chemical compound NC(=O)C1=CC=CN=C1 DFPAKSUCGFBDDF-UHFFFAOYSA-N 0.000 abstract 1
- 230000008512 biological response Effects 0.000 abstract 1
- 229960004106 citric acid Drugs 0.000 abstract 1
- 235000015165 citric acid Nutrition 0.000 abstract 1
- 229960002737 fructose Drugs 0.000 abstract 1
- 239000003607 modifier Substances 0.000 abstract 1
- 235000015277 pork Nutrition 0.000 abstract 1
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 1
- RADKZDMFGJYCBB-UHFFFAOYSA-N pyridoxal hydrochloride Natural products CC1=NC=C(CO)C(C=O)=C1O RADKZDMFGJYCBB-UHFFFAOYSA-N 0.000 abstract 1
- 238000003756 stirring Methods 0.000 abstract 1
- 235000019158 vitamin B6 Nutrition 0.000 abstract 1
- 239000011726 vitamin B6 Substances 0.000 abstract 1
- 229940011671 vitamin b6 Drugs 0.000 abstract 1
- 101710178392 Angiotensin-converting enzyme inhibitory peptide Proteins 0.000 description 8
- 235000020510 functional beverage Nutrition 0.000 description 6
- 238000003307 slaughter Methods 0.000 description 6
- 206010010774 Constipation Diseases 0.000 description 3
- 239000005715 Fructose Substances 0.000 description 3
- 208000008589 Obesity Diseases 0.000 description 3
- 235000020824 obesity Nutrition 0.000 description 3
- 230000002265 prevention Effects 0.000 description 3
- 239000008213 purified water Substances 0.000 description 3
- 238000011282 treatment Methods 0.000 description 3
- NHJVRSWLHSJWIN-UHFFFAOYSA-N 2,4,6-trinitrobenzenesulfonic acid Chemical compound OS(=O)(=O)C1=C([N+]([O-])=O)C=C([N+]([O-])=O)C=C1[N+]([O-])=O NHJVRSWLHSJWIN-UHFFFAOYSA-N 0.000 description 2
- 241000219122 Cucurbita Species 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 description 2
- 206010030113 Oedema Diseases 0.000 description 2
- 102000035195 Peptidases Human genes 0.000 description 2
- 108091005804 Peptidases Proteins 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 108010046377 Whey Proteins Proteins 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000000367 immunologic factor Substances 0.000 description 2
- 239000003112 inhibitor Substances 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 235000010234 sodium benzoate Nutrition 0.000 description 2
- 239000004299 sodium benzoate Substances 0.000 description 2
- 235000021119 whey protein Nutrition 0.000 description 2
- UUUHXMGGBIUAPW-UHFFFAOYSA-N 1-[1-[2-[[5-amino-2-[[1-[5-(diaminomethylideneamino)-2-[[1-[3-(1h-indol-3-yl)-2-[(5-oxopyrrolidine-2-carbonyl)amino]propanoyl]pyrrolidine-2-carbonyl]amino]pentanoyl]pyrrolidine-2-carbonyl]amino]-5-oxopentanoyl]amino]-3-methylpentanoyl]pyrrolidine-2-carbon Chemical compound C1CCC(C(=O)N2C(CCC2)C(O)=O)N1C(=O)C(C(C)CC)NC(=O)C(CCC(N)=O)NC(=O)C1CCCN1C(=O)C(CCCN=C(N)N)NC(=O)C1CCCN1C(=O)C(CC=1C2=CC=CC=C2NC=1)NC(=O)C1CCC(=O)N1 UUUHXMGGBIUAPW-UHFFFAOYSA-N 0.000 description 1
- 241000251468 Actinopterygii Species 0.000 description 1
- 101710086378 Bradykinin-potentiating and C-type natriuretic peptides Proteins 0.000 description 1
- 241000555825 Clupeidae Species 0.000 description 1
- 208000007530 Essential hypertension Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 108010011756 Milk Proteins Proteins 0.000 description 1
- 102000014171 Milk Proteins Human genes 0.000 description 1
- 102000004270 Peptidyl-Dipeptidase A Human genes 0.000 description 1
- 108090000882 Peptidyl-Dipeptidase A Proteins 0.000 description 1
- 208000004880 Polyuria Diseases 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 241000269821 Scombridae Species 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
- 102000007544 Whey Proteins Human genes 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000001164 bioregulatory effect Effects 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 230000001684 chronic effect Effects 0.000 description 1
- 230000001882 diuretic effect Effects 0.000 description 1
- 238000002651 drug therapy Methods 0.000 description 1
- 235000006694 eating habits Nutrition 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 235000019688 fish Nutrition 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000001641 gel filtration chromatography Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 238000000265 homogenisation Methods 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 235000020640 mackerel Nutrition 0.000 description 1
- 235000021239 milk protein Nutrition 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 208000015122 neurodegenerative disease Diseases 0.000 description 1
- 229960003512 nicotinic acid Drugs 0.000 description 1
- 235000001968 nicotinic acid Nutrition 0.000 description 1
- 239000011664 nicotinic acid Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 238000011321 prophylaxis Methods 0.000 description 1
- 235000019833 protease Nutrition 0.000 description 1
- 235000019419 proteases Nutrition 0.000 description 1
- 235000019512 sardine Nutrition 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 1
- 229940033282 vitamin b6 2 mg Drugs 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
- A23L2/60—Sweeteners
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2250/00—Food ingredients
- A23V2250/70—Vitamins
Landscapes
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Non-Alcoholic Beverages (AREA)
Abstract
Description
본 발명은 현재 기능성 음료 및 식품이 많이 개발되고 있는 추세에 따라 보다 저렴하고도 효과적인 생체조절 물질을 함유하는 성인병 예방 목적 기능성 음료를 개발하고자 기 취득한 특허 (등록번호: 10-0215090, 특허권자: 송경빈)의 소, 돼지 혈장단백질 가수분해물이 고혈압 억제 관련 엔지오텐신전환효소저해 펩타이드를 다량 함유하고 있는 바 최적의 가수분해도를 갖는 혈장단백질 가수분해물에 변비예방, 이뇨작용, 부종의 치료, 비만 방지 등의 효과가 알려져 있는 늙은 호박 엑스를 첨가하고 감미제, 비타민류, 안정화제, 착향제, 산미제, 보존제 등을 배합하여 균질화, 여과, 멸균하여 성인병 예방 생체조절물질 함유 기능성음료를 제조하는데 그 주요 목적이 있다.The present invention is a patent that has already been acquired to develop a functional drink for the purpose of preventing adult diseases containing a cheaper and more effective bioregulatory material in accordance with the trend of the development of many functional drinks and foods (registration number: 10-0215090, patent holder: Kyung-bin Song) Bovine and porcine plasma protein hydrolysates contain large amounts of angiotensin-converting enzyme inhibitory peptides related to hypertension, and thus, constipation prevention, diuretic effect, treatment of edema, prevention of obesity, etc. Its main purpose is to make functional beverages containing biomodulators to prevent adult diseases by adding homogenous pumpkin extract and known as a sweetener, vitamins, stabilizers, flavoring agents, acidulants and preservatives. .
성인병 중 대표적인 고혈압은 순환기계통의 만성 퇴행성질환으로서 뇌졸중과 관련하여 우리나라에서 사망원인의 1위를 차지하고 있다. 고혈압의 90%는 본태성 고혈압으로서 그 발병원인이 밝혀져 있지 않으나 유전적인 요인과 비만, 스트레스, 식이 습관 등 환경 요인에 의하여 발병하는 것으로 알려져 있다. 고혈압의 치료로 사용되는 약물요법은 부작용을 수반하는 이유로 보다 안전하고 예방적인 치료가 필요하다. 근래 식품으로부터 혈압상승과 관련되는 엔지오텐신전환효소 (angiotensin converting enzyme) 저해 펩타이드 관련 연구가 정어리, 고등어 등 생선류를 포함하여 우유단백질, 유청단백질 등에서 엔지오텐신전환효소 저해 펩타이드를 분리하는 연구가 이루어져 미국 등에서는 이미 엔지오텐신전환효소 저해 펩타이드를 함유하는 유청단백질 가수분해물을 사용하여 기능성 음료를 개발하여 판매하고 있다. 그러나 현재 우리나라에서는 이와같은 엔지오텐신전환효소 저해 펩타이드를 함유하는 기능성 음료가 개발되지 않고 있다.Representative hypertension among adult diseases is chronic degenerative disease of circulatory system and is the leading cause of death in Korea in relation to stroke. Although 90% of hypertension is essential hypertension, its cause is not known, but it is known to be caused by environmental factors such as genetic factors, obesity, stress, and dietary habits. Drug therapy, which is used to treat high blood pressure, requires safer and more prophylactic treatment for its side effects. In recent years, studies related to angiotensin converting enzyme inhibitory peptides related to blood pressure rise have been conducted to isolate angiotensin converting enzyme inhibitory peptides from milk proteins and whey proteins including fish such as sardines and mackerel. Has already developed and marketed functional beverages using whey protein hydrolysates containing angiotensin converting enzyme inhibitory peptides. However, at present, functional beverages containing such angiotensin converting enzyme inhibitory peptide have not been developed.
본 발명은 현재 도축장에서 도축 후 그 처리 문제에 있어 폐수 방류 등 환경오염 문제가 심각한 소, 돼지 도축 혈액의 효율적인 이용 방안으로서 기존 연구되었던 도축혈액으로부터 고혈압 관련 엔지오텐신전환효소 저해 펩타이드 분리 정제 방법 (등록번호: 10-0215090, 특허권자: 송경빈)을 응용한 기술이다. 국내 도축장에서 발생되는 소, 돼지 도축혈액은 현재 선지, 순대 등 일부만이 이용되고 대부분이 폐기되고 있다. 외국의 경우에도 도축혈액을 건조하여 동물사료, 비료 등에 대부분 이용될 뿐 혈장 단백질의 그 영양적 가치와 관련하여 고부가가치 창출은 이루어지지 않고 있으며 따라서 현재 세계적으로 혈장단백질 가수분해물을 이용한 음료는 아직 없다. 그러므로 본 발명의 기술은 국내외적으로 최초로 개발되는 기술로서의 그 의의가 있다.The present invention is a method for separating and purifying high blood pressure-related angiotensin converting enzyme inhibitor peptide from slaughter blood, which has been studied previously as an efficient method for the use of slaughtered blood from cattle and pigs that have serious environmental pollution problems such as wastewater discharge in the slaughterhouse. No .: 10-0215090, Patent holder: Song Kyung-bin). Cattle and pig slaughter blood from domestic slaughterhouses is currently only partially used, including seonji and sundae, and most of them are discarded. Even in foreign countries, slaughter blood is dried and used mostly in animal feed and fertilizers. However, high value-added products have not been created in relation to the nutritional value of plasma proteins. . Therefore, the technology of the present invention is meaningful as a technology first developed at home and abroad.
본 발명의 목적은 소, 돼지 도축혈액의 혈장단백질 가수분해물로부터 고혈압 관련 엔지오텐신전환효소 저해 펩타이드를 함유하는 기능성 음료를 개발하는데 있다. 따라서 상기 목적을 달성하기 위하여 본 발명의 특징은 도축혈액의 혈장단백질을 수거하는 방법, 상업용 단백분해효소를 이용하여 가장 이상적인 가수분해도 즉 엔지오텐신전환효소 저해도가 가장 높은 최적 조건을 갖게 하는 방법, 부재료로 사용된 늙은 호박 엑스를 첨가하여 풍미 및 기능성을 보다 첨가한 음료로서의 제조 방법에 있다.An object of the present invention is to develop a functional beverage containing high blood pressure related angiotensin converting enzyme inhibitory peptide from plasma protein hydrolysates of bovine and porcine slaughtered blood. Therefore, in order to achieve the above object, a feature of the present invention is a method of collecting plasma proteins of slaughtered blood, a method of having an optimal condition having the highest ideal degree of hydrolysis, ie, angiotensin converting enzyme inhibition using commercial protease, It is in the manufacturing method as a beverage which added the flavor and functionality further by adding the old pumpkin extract used as a submaterial.
본 발명은 도축장에서 도축혈액의 수거, 혈장의 분리, 고혈압 저해물질인 엔지오텐신전환효소저해 펩타이드를 다량 함유하고 있는 최적의 가수분해도를 갖는 혈장단백질 가수분해물의 제조, 늙은 호박 엑스, 감미제, 비타민류, 안정화제, 착향제, 산미제, 보존제 등을 첨가하여 균질화, 여과, 멸균, 포장하여 성인병 예방 생체조절물질 함유 기능성음료를 개발하는 내용으로 이루어져 있다.The present invention is a plasma protein hydrolyzate having an optimal degree of hydrolysis containing a large amount of angiotensin-converting enzyme inhibitory peptide, a high blood pressure inhibitor, the collection of slaughtered blood in the slaughterhouse, old pumpkin x, sweeteners, vitamins , Stabilizer, flavoring agent, acidulant, preservative, etc. by adding homogenization, filtration, sterilization, packaging to develop a functional beverage containing biomodulators to prevent adult diseases.
본 발명을 실시예를 통해서 설명하고자 한다.The present invention will be described through examples.
실시예 1 (도축혈액 혈장단백질 가수분해물의 제조 방법)Example 1 (Method of Producing Slaughtered Plasma Protein Hydrolyzate)
도축혈액은 도축 후 즉시 위생적으로 수거된 후 연속식 원심분리기로 혈장만을 분리한 후 2%-10% 트리클로로아세트산, 0.6N-3N 염산, 50%-70% 황산암모늄 등으로 혈장단백질을 침전시킨다. 원심분리 후 침전된 혈장단백질은 95 0C에서 20분간 열처리한 후 상업용 단백분해효소인 플라보자임 (Flavorzyme)의 경우 1%, 50℃, pH 7.0, 알칼레이즈 (Alcalase)의 경우 1%, 55℃, pH 8.0 조건에서 2-6시간 동안 가수분해를 하여 트리니트로벤젠설포닉에시드 (TNBS) 에세이와 겔여과크로마토그라피 (GPC)로 측정하여 가수분해도 (degree of hydrolysis)가 가수분해물의 분자량 분포에 있어 분자량 1000-2000 Da 이하가 20-40%가 되게 한다. 가수분해물은 멤브레인 (분자량 한계 5000)으로 1차만 한외여과하거나 또는 분자량 한계 1000으로 2차 한외여과까지 한 후 여과액을 분무건조 또는 동결건조하여 도축혈액 혈장단백질 가수분해물을 제조한다.Slaughtered blood is collected hygienically immediately after slaughter, and then plasma is separated by a continuous centrifuge. Precipitate plasma proteins with 2% -10% trichloroacetic acid, 0.6N-3N hydrochloric acid, and 50% -70% ammonium sulfate. . Plasma proteins precipitated after centrifugation were heat-treated at 95 0 C for 20 minutes, and then 1% for commercial proteinase Flavorzyme, 50 ° C, pH 7.0, 1% for Alcalase and 55%. Hydrolysis was carried out for 2-6 hours at pH 8.0 and measured by trinitrobenzenesulfonic acid (TNBS) assay and gel filtration chromatography (GPC) to determine the degree of hydrolysis in the molecular weight distribution of the hydrolyzate. The molecular weight is 1000-2000 Da or less to 20-40%. The hydrolyzate is ultrafiltration only with the membrane (molecular weight limit 5000) or the second ultrafiltration with a molecular weight limit of 1000, and then the filtrate is spray dried or lyophilized to prepare the slaughtered plasma protein hydrolyzate.
실시예 2 (기능성음료의 제조 방법)Example 2 (Manufacturing Method of Functional Drink)
일정 정제수에 도축혈액 혈장단백질 가수분해물을 가하고 늙은 호박을 세척, 탈피, 절단, 씨 제거한 후 90-100℃에서 4시간 열수 추출하여 100-200 메쉬 (mesh)에서 여과하여 얻어진 늙은 호박 엑스를 넣고 60℃에서 교반하여 혼화한 후 액상과당 등 감미제 1-10%, 구연산 등 산미제 0.1-0.05%, 강화제로서 비타민 B6, B2, C, 니콘틴산아미드를 0.001-0.1%, 혼합과일향 등 착향제 0.1%, 안식향산나트륨 등 보존제 0.05-0.1% 가하고 용해시킨 후 폴리솔베이트 등 안정화제를 첨가하여 균질기로 균질화하고 100 -200 메쉬에서 1차 여과, 1-2μ에서 2차 여과한 후 정제수를 넣어 전체량을 맞춘 후 순간 멸균기에서 90-100℃에서 45-60초간 멸균 후 자동충전기에서 병 또는 통조림에 충전하여 제조한다.Add slaughter blood plasma protein hydrolyzate to purified water, wash, peel, cut, and remove old pumpkins, extract hot water at 90-100 ° C for 4 hours, and add old pumpkin extracts obtained by filtration at 100-200 mesh. After mixing at room temperature by mixing at 1 ° C, sweeteners such as liquid fructose, sweeteners such as fructose, 0.1-0.05% of citric acid, etc. %, Preservatives such as sodium benzoate, 0.05-0.1% were added and dissolved, homogenized with a homogenizer by adding stabilizers such as polysorbate, primary filtration at 100-200 mesh, secondary filtration at 1-2μ, and then purified water After the sterilization for 45-60 seconds at 90-100 ℃ in the instant sterilizer after filling the bottle or canned in an automatic charger is prepared.
본 제품의 대표적인 구성비는 다음과 같다.Typical composition ratio of this product is as follows.
100ml 중In 100 ml
도축혈액 혈장단백질 가수분해물 100mgSlaughter Blood Plasma Protein Hydrolyzate 100mg
늙은 호박 엑스 50mgOld Pumpkin X 50mg
비타민 B6 2mgVitamin B6 2mg
비타민 B2 2mgVitamin B2 2mg
비타민 C 10mgVitamin C 10mg
니콘틴산아미드 2mg2 mg of nicotinic acid
구연산 100mgCitric acid 100mg
액상과당 10g10g of liquid fructose
안식향산나트륨 50mg50 mg sodium benzoate
혼합과일향 100mgMixed Fruit Flavor 100mg
NaCl 10mgNaCl 10mg
폴리솔베이트 25mgPolysorbate 25mg
정제수 적량Purified water
최종 제품: pH 3.25, 비중 1.02Final product: pH 3.25, specific gravity 1.02
본 발명의 효과는 도축 혈액의 효율적인 처리 방안 제시와 더불어 혈장단백질 가수분해물이 고혈압 저해물질인 엔지오텐신전환효소 저해 펩타이드를 다량 함유하고 있는 바 최적의 가수분해도를 갖는 혈장단백질 가수분해물에 변비예방, 이뇨작용, 부종의 치료, 비만 방지 등의 효과가 알려져 있는 늙은 호박 엑스 등 부재룔를 첨가하고 감미제, 비타민류, 안정화제, 착향제, 산미제, 보존제 등을 배합하여 균질화, 여과, 멸균하여 성인병 예방 생체조절물질 함유 기능성음료를 개발하는데 있다.The present invention provides an efficient treatment method for slaughtered blood and prevents constipation and prevents constipation in plasma protein hydrolyzate having optimal hydrolysis, since the plasma protein hydrolyzate contains a large amount of angiotensin converting enzyme inhibitory peptide, which is a hypertension inhibitor. Prevents adult diseases by adding homogenizers, vitamins, stabilizers, flavoring agents, acidulants, preservatives, etc. by adding absent pumpkins, etc., which are known to have effects, treatment of edema, and prevention of obesity. To develop functional beverages containing modulators.
Claims (3)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR10-2000-0045023A KR100370272B1 (en) | 2000-08-03 | 2000-08-03 | Manufacture of functional drink using bovine and porcine blood plasma protein hydrolysates |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR10-2000-0045023A KR100370272B1 (en) | 2000-08-03 | 2000-08-03 | Manufacture of functional drink using bovine and porcine blood plasma protein hydrolysates |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| KR20000063783A true KR20000063783A (en) | 2000-11-06 |
| KR100370272B1 KR100370272B1 (en) | 2003-01-30 |
Family
ID=19681568
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR10-2000-0045023A KR100370272B1 (en) | 2000-08-03 | 2000-08-03 | Manufacture of functional drink using bovine and porcine blood plasma protein hydrolysates |
Country Status (1)
| Country | Link |
|---|---|
| KR (1) | KR100370272B1 (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20210093620A (en) * | 2020-01-20 | 2021-07-28 | 조선대학교산학협력단 | Feed additive composition for eel containing probiotics and pig plasma hydrolyzate as an active ingredient |
| KR20210145915A (en) * | 2020-05-26 | 2021-12-03 | 유석원 | Cultivating method for crops with high mineral contents |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104432325A (en) * | 2014-12-09 | 2015-03-25 | 山东龙力生物科技股份有限公司 | Pumpkin juice with hpyerglycemic function and preparation method of pumpkin juice |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH01273560A (en) * | 1988-04-23 | 1989-11-01 | Ichimaru Pharcos Co Ltd | Hem iron solution or beverage containing the same solution |
| KR910005622B1 (en) * | 1989-11-22 | 1991-08-01 | 한국 식품개발 연구원 | Product of food usable blood of stock |
| KR100215090B1 (en) * | 1996-10-14 | 1999-08-16 | 송경빈 | Process for preparing angiotensin converting enzyme inhibitor from plasma protein hydrolysate |
| KR19980065955A (en) * | 1997-01-17 | 1998-10-15 | 강효동 | In-vehicle safety equipment |
| KR101090478B1 (en) * | 2005-12-22 | 2011-12-08 | 엘지전자 주식회사 | Indoor unit of picture frame type air-conditioner |
| KR102124853B1 (en) * | 2018-12-14 | 2020-06-19 | 건국대학교 산학협력단 | UV-curable urethane acrylate polymer using mixture of polyester polyols and process for the preparation thereof |
| KR102125390B1 (en) * | 2019-01-30 | 2020-06-22 | 한국과학기술원 | Absorptive optical filter having glass cloth transparent and manufacturing method thereof |
-
2000
- 2000-08-03 KR KR10-2000-0045023A patent/KR100370272B1/en active IP Right Grant
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20210093620A (en) * | 2020-01-20 | 2021-07-28 | 조선대학교산학협력단 | Feed additive composition for eel containing probiotics and pig plasma hydrolyzate as an active ingredient |
| KR20210145915A (en) * | 2020-05-26 | 2021-12-03 | 유석원 | Cultivating method for crops with high mineral contents |
Also Published As
| Publication number | Publication date |
|---|---|
| KR100370272B1 (en) | 2003-01-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| RU2292155C2 (en) | Method for milk proteins hydrolysis | |
| JP3236011B2 (en) | Hypoallergenic dairy product and method for producing the same | |
| US6737076B2 (en) | Hypoallergenic composition containing tolerogenic peptides | |
| US8940685B2 (en) | Method for preparing active peptides from corn germ proteins | |
| US4980450A (en) | Phosphopeptides from casein-based raw material | |
| Mirdhayati et al. | Angiotensin converting enzyme (ACE) inhibitory and antihypertensive activities of protein hydrolysate from meat of Kacang goat (Capra aegagrus hircus) | |
| AU2009332369B2 (en) | Muscle-building agent | |
| CN102076221B (en) | The product for preparing the method for wrapped, the hot preserved aqueous drink comprising casein micelles and the peptide rich in tryptophan and thereby obtaining | |
| NZ245031A (en) | Casein hydrolysate and its enzymatic production. | |
| CN108410938A (en) | A method of preparing low bitter taste lactalbumin antioxidant peptide powder | |
| ES2964808T3 (en) | Palatable Extensively Hydrolyzed Whey Protein Hydrolysates | |
| JP2008511317A (en) | ACE-inhibited whey hydrolyzate | |
| KR101655540B1 (en) | The low sodium fish sauces and method for preparing the same | |
| KR100370272B1 (en) | Manufacture of functional drink using bovine and porcine blood plasma protein hydrolysates | |
| AU2010307691B2 (en) | Fat accumulation suppressor | |
| KR100946997B1 (en) | A composition containings hydrolysates, second hydrolysates or isolated active peptides of Theragra chalcogramma as an active ingredient | |
| US5219735A (en) | Non-phosphorylated peptides from casein-based material | |
| Geirsdóttir | Isolation, purification and investigation of peptides from fish proteins with blood pressure decreasing properties | |
| JP2887302B2 (en) | Casein phospho-oligopeptide mixture, method for producing the same, and health food containing the mixture | |
| KR102214600B1 (en) | Functional composition comprsing low molecular collagen peptide extract from skate skin and Functional food comprising the same | |
| JPH10279595A (en) | Yolk low-molecular peptide | |
| KR19980074162A (en) | Recovery Method of Immune Protein for Food Addition Improved Off-flavor and Color Selection from Livestock Blood |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A201 | Request for examination | ||
| E902 | Notification of reason for refusal | ||
| E701 | Decision to grant or registration of patent right | ||
| GRNT | Written decision to grant | ||
| FPAY | Annual fee payment |
Payment date: 20130102 Year of fee payment: 11 |
|
| FPAY | Annual fee payment |
Payment date: 20130225 Year of fee payment: 18 |