KR102214600B1 - Functional composition comprsing low molecular collagen peptide extract from skate skin and Functional food comprising the same - Google Patents
Functional composition comprsing low molecular collagen peptide extract from skate skin and Functional food comprising the same Download PDFInfo
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- KR102214600B1 KR102214600B1 KR1020190000460A KR20190000460A KR102214600B1 KR 102214600 B1 KR102214600 B1 KR 102214600B1 KR 1020190000460 A KR1020190000460 A KR 1020190000460A KR 20190000460 A KR20190000460 A KR 20190000460A KR 102214600 B1 KR102214600 B1 KR 102214600B1
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/20—Reducing nutritive value; Dietetic products with reduced nutritive value
- A23L33/21—Addition of substantially indigestible substances, e.g. dietary fibres
- A23L33/28—Substances of animal origin, e.g. gelatin or collagen
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
- A23V2200/30—Foods, ingredients or supplements having a functional effect on health
- A23V2200/332—Promoters of weight control and weight loss
- A23V2200/3324—Low fat - reduced fat content
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- Zoology (AREA)
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- Chemical & Material Sciences (AREA)
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Abstract
본 발명에 따른 체지방 감소 기능성 원료 조성물은, 홍어껍질을 가수분해하여 얻은 저분자 콜라겐 펩타이드를 유효성분으로 포함하는 체지방 감소 기능성 원료 조성물로서, 단백질 함량이 90 중량% 이상이고, 하기 화학식 1로 표현되는 제1 화합물 58~89 mg/g 및 하기 화학식 2로 표현되는 제2 화합물 190~286 mg/g 을 함유한 저분자 콜라겐 펩타이드를 유효성분으로 포함하되,
상기 저분자 콜라겐 펩타이드는 홍어가공 공정 중에 발생된 홍어 껍질 원재료(홍어 껍질 100%로 이루어짐)를 수집하여 냉동보관하는 단계; 껍질을 고압반응기에 넣고 121℃에서 90분간 고압열수추출하는 단계; 단백질 가수분해 효소인 Alcalase와 Protamax을 투입원료 대비 각각 0.5%를 2시간 동안 투입하여 가수분해하는 단계; 활성탄 및 이온교환수지를 이용하여 탈취하는 단계; 감압여과를 통해 불순물을 제거하는 단계; 및 분자량 1,000Da.를 기준으로 컷오프한 후 농축 및 동결건조하는 단계; 를 거쳐 제조되는 것을 특징으로 한다.
[화학식 1]
[화학식 2]
The body fat reduction functional raw material composition according to the present invention is a body fat reduction functional raw material composition comprising a low molecular weight collagen peptide obtained by hydrolyzing skate skin as an active ingredient, and has a protein content of 90% by weight or more, and is represented by the following formula (1). 1 compound 58-89 mg/g and a low molecular weight collagen peptide containing 190-286 mg/g of the second compound represented by the following formula (2) as an active ingredient,
The low molecular weight collagen peptide is a step of collecting and freezing the raw materials of the skate skin (consisting of 100% skate skin) generated during the skate processing process; Putting the skin in a high-pressure reactor and extracting hot water at 121° C. for 90 minutes; Hydrolyzing the proteolytic enzymes Alcalase and Protamax by adding 0.5% of each of the raw materials for 2 hours; Deodorizing using activated carbon and ion exchange resin; Removing impurities through vacuum filtration; And concentrating and lyophilizing after cutting off based on a molecular weight of 1,000 Da. It characterized in that it is manufactured through.
[Formula 1]
[Formula 2]
Description
본 발명은 홍어껍질을 가수분해하여 얻은 저분자 콜라겐 펩타이드를 유효성분으로 포함하는 체지방 감소 기능성 원료 조성물에 관한 것으로, 홍어껍질 유래 저분자 콜라겐 펩타이드의 전임상 및 인체적용실험을 통한 체지방 감소 기능성 원료 개발 및 산업화에 관한 것이다. The present invention relates to a body fat reduction functional raw material composition comprising a low molecular weight collagen peptide obtained by hydrolyzing skate skin as an active ingredient, and for the development and industrialization of functional raw materials for reducing body fat through preclinical and human application experiments of low molecular weight collagen peptide derived from skate skin. About.
홍어(Skate)는 우리나라에서 즐겨먹는 기호 식품으로 소화기능 및 식욕증진, 면역능력 향상 및 노화방지, 피부탄력 유지 및 관절염 예방 등 다양한 약리적인 효능을 지니고 있으며, 현재까지 홍어의 소비 및 생산 방식은 홍어회, 홍어무침, 홍어 애(간) 등과 같이 특별한 가공 없이 섭취하는 형태이다. Skate is a favorite food in Korea and has various pharmacological effects such as digestive function and appetite enhancement, immune function improvement and aging prevention, skin elasticity maintenance, and arthritis prevention. It is a form that is consumed without special processing, such as, scallop stew, and skatefish (liver).
홍어의 기능적 특성과 특유의 기호성으로 인하여 과거 전남 지방에서만 소량 소비되는 형태에서 벗어나 전국적으로 그 수요가 급증하여 현재에는 대형마트, 홈쇼핑 등에서도 지속적으로 대량 소비되고 있다. Due to the functional characteristics and peculiar palatability of skates, the demand for skates has rapidly increased nationwide, from the form of being consumed only in small quantities in Jeollanam-do.
어류의 껍질과 내장 조직에는 다량의 콜라겐 성분이 함유되어 있으며, 기존에 소나 돼지 등의 껍질, 뼈 등에서 유래하는 콜라겐과 달리 광우병이나 구제역 등과 같은 인체 전이 위험성을 가지지 있지 않기에 안전하다고 보고되고 있다.Fish skin and internal tissues contain a large amount of collagen, and it is reported that it is safe because it does not have the risk of human metastasis such as mad cow disease or foot-and-mouth disease, unlike collagen derived from the skin and bones of cattle and pigs.
홍어껍질에는 다량의 콜라겐이 함유되어 있어, 이를 고부가가치의 기능성 식품소재로서 이용이 가능하다. 그러나, 콜라겐 자체로는 분자량이 크고(분자량 30kDa. 이상인 것으로 알려짐), 물에 녹지 않아 소화 흡수율이 낮기 때문에 체내 흡수력을 극대화를 위해서 저분자화시킨 저분자 펩타이드가 필요하다.Since the skate skin contains a large amount of collagen, it can be used as a functional food material with high added value. However, since collagen itself has a large molecular weight (known to have a molecular weight of 30 kDa or more), and is insoluble in water and has a low digestion and absorption rate, a low-molecular peptide is required to maximize absorption in the body.
본 발명은 상기와 같은 문제점을 해결하기 위해 안출한 것으로, 본 발명의 목적은 홍어껍질을 가수분해하여 얻은 저분자 콜라겐 펩타이드를 유효성분으로 포함하는 체지방 감소 기능성 원료 조성물을 제공함에 있다. The present invention has been conceived to solve the above problems, and an object of the present invention is to provide a functional raw material composition for reducing body fat comprising a low molecular weight collagen peptide obtained by hydrolyzing skate skin as an active ingredient.
또한 본 발명은 저분자 콜라겐 펩타이드 제조를 위하여 홍어 가공시 발생되는 부산물인 껍질을 이용하여 분자량 별로 콜라겐 펩타이드를 추출하고, 추출물을 이용한 산업적 활용 가능성을 확인하기 위한 실험으로 일반성분 및 분자량, 아미노산 조성, 탈취 등의 실험을 통해 산업적 적용 가능성을 검토함에 있다. In addition, the present invention is an experiment to extract collagen peptides by molecular weight by using the skin, a by-product generated during processing of skates, and to confirm the industrial applicability using the extract for the production of low molecular weight collagen peptides.General ingredients and molecular weight, amino acid composition, and deodorization It is to examine the possibility of industrial application through experiments such as.
본 발명이 이루고자 하는 기술적 과제들은 이상에서 언급한 기술적 과제들로 제한되지 않는다.The technical problems to be achieved by the present invention are not limited to the technical problems mentioned above.
이와 같은 목적을 달성하기 위하여, 본 발명의 일 실시예에 따른 체지방 감소 기능성 원료 조성물은, 홍어껍질을 가수분해하여 얻은 저분자 콜라겐 펩타이드를 유효성분으로 포함하는 체지방 감소 기능성 원료 조성물로서, 단백질 함량이 90 중량% 이상이고, 하기 화학식 1로 표현되는 제1 화합물 58~89 mg/g 및 하기 화학식 2로 표현되는 제2 화합물 190~286 mg/g 을 함유한 저분자 콜라겐 펩타이드를 유효성분으로 포함하되,
상기 저분자 콜라겐 펩타이드는 홍어가공 공정 중에 발생된 홍어 껍질 원재료(홍어 껍질 100%로 이루어짐)를 수집하여 냉동보관하는 단계; 껍질을 고압반응기에 넣고 121℃에서 90분간 고압열수추출하는 단계; 단백질 가수분해 효소인 Alcalase와 Protamax을 투입원료 대비 각각 0.5%를 2시간 동안 투입하여 가수분해하는 단계; 활성탄 및 이온교환수지를 이용하여 탈취하는 단계; 감압여과를 통해 불순물을 제거하는 단계; 및 분자량 1,000Da.를 기준으로 컷오프한 후 농축 및 동결건조하는 단계; 를 거쳐 제조되는 것을 특징으로 한다. In order to achieve this object, the body fat reduction functional raw material composition according to an embodiment of the present invention is a body fat reduction functional raw material composition comprising a low molecular weight collagen peptide obtained by hydrolysis of skate skin as an active ingredient, and has a protein content of 90 A low molecular weight collagen peptide containing 58 to 89 mg/g of the first compound represented by the following formula (1) and 190 to 286 mg/g of the second compound represented by the following formula (2) as an active ingredient,
The low molecular weight collagen peptide is a step of collecting and freezing the raw materials of the skate skin (consisting of 100% skate skin) generated during the skate processing process; Putting the skin in a high-pressure reactor and extracting hot water at 121° C. for 90 minutes; Hydrolyzing the proteolytic enzymes Alcalase and Protamax by adding 0.5% of each of the raw materials for 2 hours; Deodorizing using activated carbon and ion exchange resin; Removing impurities through vacuum filtration; And concentrating and lyophilizing after cutting off based on a molecular weight of 1,000 Da. It characterized in that it is manufactured through.
삭제delete
[화학식 1][Formula 1]
[화학식 2][Formula 2]
본 발명의 일 실시예에 따른 체지방 감소 기능성 원료 조성물에 있어, 상기 저분자 콜라겐 펩타이드의 유리 아미노산은 상기 유리 아미노산 전체 100 중량%을 기준으로 하여 상기 제1 화합물 6~8 중량% 및 상기 제2 화합물 19~23 중량%를 포함할 수 있다. In the body fat reduction functional raw material composition according to an embodiment of the present invention, the free amino acid of the low molecular weight collagen peptide is 6 to 8% by weight of the first compound and the second compound 19 based on 100% by weight of the total free amino acids. It may contain ~23% by weight.
일 예로서, 상기 제1 화합물은 Hydroxy proline일 수 있고, 상기 제2 화합물은 Glycine일 수 있다. As an example, the first compound may be Hydroxy proline, and the second compound may be Glycine.
본 발명의 일 실시예에 따른 체지방 감소 기능성 원료 조성물에 있어, 상기 저분자 콜라겐 펩타이드의 구성 아미노산은 상기 구성 아미노산 전체 100 중량%를 기준으로 Aaspartic acid 5.36~5.90 중량%, Threonine 3.38~3.72 중량%, Serine 9.77~10.71 중량%, Glutamic acid 9.77~10.71 중량%, Glycine 21.27~23.64 중량%, Alanine 7.65~8.51 중량%, Valine 2.88~3.17 중량%, Methionine 1.72~2.16 중량%, Isoleucine 2.34~2.58 중량%, Leucine 3.50~3.87 중량%, Tyrosine 0.73~0.86 중량%, Phenylalanine 2.07~2.28 중량%, Lysine 3.54~3.88 중량%, Ammonia 0.84~0.92 중량%, Histidine 1.49~1.62 중량% 및 Arginine 7.11~7.93 중량%을 포함할 수 있다. In the body fat reduction functional raw material composition according to an embodiment of the present invention, the constituent amino acids of the low-molecular collagen peptide are 5.36 to 5.90 wt% of Aaspartic acid, 3.38 to 3.72 wt% of Threonine, and Serine based on 100 wt% of the total of the constituent amino acids. 9.77~10.71 wt%, Glutamic acid 9.77~10.71 wt%, Glycine 21.27~23.64 wt%, Alanine 7.65~8.51 wt%, Valine 2.88~3.17 wt%, Methionine 1.72~2.16 wt%, Isoleucine 2.34~2.58 wt%, Leucine 3.50 to 3.87 wt%, Tyrosine 0.73 to 0.86 wt%, Phenylalanine 2.07 to 2.28 wt%, Lysine 3.54 to 3.88 wt%, Ammonia 0.84 to 0.92 wt%, Histidine 1.49 to 1.62 wt% and Arginine 7.11 to 7.93 wt% I can.
본 발명의 일 실시예에 따른 체지방 감소 기능성 원료 조성물에 있어, 상기 저분자 콜라겐 펩타이드의 유리 아미노산은 상기 유리 아미노산 전체 100 중량%를 기준으로 Phosphoserine 0.03~0.05 중량%, Taurine 0.01~0.02 중량%, Aspartic acid 6.46~7.11 중량%, Hydroxy proline 6.10~6.85 중량%, Threonine 3.29~3.42 중량%, Serine 5.38~5.71 중량%, Glutamic acid 9.93~10.78 중량%, Proline 8.28~9.02 중량%, Glycine 19.97~22.09 중량%, Alanine 6.92~7.66 중량%, Valine 2.93~3.34 중량%, Methionine 1.67~2.15 중량%, Isoleucine 2.20~2.45 중량%, Leucine 3.32~3.67 중량%, Tyrosine 0.81~0.91 중량%, Phenylalanine 2.02~2.23 중량%, Ammonia 0.71~0.74 중량%, Hyroxylysine 0.09~1.05 중량%, Ornithine 0.03~0.04 중량%, Lysine 3.22~3.49 중량%, Histidine 1.26~1.38 중량% 및 Arginine 7.05~7.84 중량%를 포함할 수 있다. In the functional raw material composition for reducing body fat according to an embodiment of the present invention, the free amino acid of the low molecular weight collagen peptide is 0.03 to 0.05% by weight of Phosphoserine, 0.01 to 0.02% by weight of Taurine, and Aspartic acid based on 100% by weight of the total free amino acid. 6.46~7.11 wt%, Hydroxy proline 6.10~6.85 wt%, Threonine 3.29~3.42 wt%, Serine 5.38~5.71 wt%, Glutamic acid 9.93~10.78 wt%, Proline 8.28~9.02 wt%, Glycine 19.97~22.09 wt%, Alanine 6.92~7.66 wt%, Valine 2.93~3.34 wt%, Methionine 1.67~2.15 wt%, Isoleucine 2.20~2.45 wt%, Leucine 3.32~3.67 wt%, Tyrosine 0.81~0.91 wt%, Phenylalanine 2.02~2.23 wt%, Ammonia 0.71 to 0.74 wt%, Hyroxylysine 0.09 to 1.05 wt%, Ornithine 0.03 to 0.04 wt%, Lysine 3.22 to 3.49 wt%, Histidine 1.26 to 1.38 wt%, and Arginine 7.05 to 7.84 wt%.
또한 본 발명은 상술한 체지방 감소 기능성 원료 조성물을 포함하는 기능성 식품을 제공한다. In addition, the present invention provides a functional food comprising the above-described body fat reduction functional raw material composition.
본 발명에 따른 체지방 감소 기능성 원료 조성물은 체내 흡수력을 극대화시킨 체지방 감소 효과를 향상시킬 수 있다. The functional raw material composition for reducing body fat according to the present invention can improve the effect of reducing body fat by maximizing absorption in the body.
또한 본 발명에 따른 기능성 식품을 중/장기적으로 복용하였을 때 체지방 감소에 효과를 줄 수 있고, 성인 기준 2,000mg/day을 중·장기간 동안 섭취하여도 안전성이 있다. In addition, when the functional food according to the present invention is taken for a medium/long term, it may have an effect on reducing body fat, and it is safe even if 2,000 mg/day for adults is consumed for a medium/long term.
또한 본 발명에 따른 체지방 감소 기능성 원료 조성물은 대량생산에도 불구하고, 제품의 수율은 약 17% 정도로 나타났는데, 홍어 껍질 원료의 수분 함량을 고려하였을 때 상당히 높은 수율을 나타낸다. In addition, despite the mass production of the functional raw material composition for reducing body fat according to the present invention, the yield of the product was about 17%, which shows a considerably high yield when considering the moisture content of the raw material of skate skin.
또한, 본 발명은 이용도가 극히 낮아 폐기 처리 되던 홍어의 부산물을 이용하여 콜라겐 펩타이드를 추출하기 때문에 자원의 낭비를 막는 경제성과 환경오염의 원인을 줄일 수 있는 효과가 있다.In addition, since the present invention extracts collagen peptides using by-products of skateboards that have been disposed of due to their extremely low availability, there is an effect of preventing waste of resources and reducing the cause of environmental pollution.
도 1은 본 발명의 일 실시예에 따른 체지방 감소 기능성 원료 조성물의 제조공정을 도시한 공정 순서도이다.
도 2는 본 발명의 일 실시예에 따른 체지방 감소 기능성 원료 조성물의 제조공정에 따라 나타난 함량변화, 수율을 도시한 도면이다. 1 is a process flow chart showing a manufacturing process of a functional raw material composition for reducing body fat according to an embodiment of the present invention.
2 is a view showing a change in content and yield according to a manufacturing process of a functional raw material composition for reducing body fat according to an embodiment of the present invention.
이하 본 발명에 관하여 상세히 설명한다. 다음에 소개되는 실시예 및 도면들은 당업자에게 본 발명의 사상이 충분히 전달될 수 있도록 하기 위해 예로서 제공되는 것이다. 또한, 본 발명의 사용되는 기술 용어 및 과학 용어에 있어서 다른 정의가 없다면, 이 발명이 속하는 기술 분야에서 통상의 지식을 가진 자가 통상적으로 이해하고 있는 의미를 가지며, 하기의 설명 및 첨부 도면에서 본 발명의 요지를 불필요하게 흐릴 수 있는 공지 기능 및 구성에 대한 설명은 생략한다.Hereinafter, the present invention will be described in detail. The following embodiments and drawings are provided as examples in order to sufficiently convey the spirit of the present invention to those skilled in the art. In addition, unless there are other definitions in the technical and scientific terms used in the present invention, the present invention has the meanings commonly understood by those of ordinary skill in the art to which this invention belongs, and the present invention in the following description and accompanying drawings Descriptions of known functions and configurations that may unnecessarily obscure the gist of are omitted.
본 발명을 서술함에 있어, 저분자 콜라겐 펩타이드는 약 500 내지 2,000 Da. 분자량을 가지는 펩타이드를 의미한다. In describing the present invention, the low molecular weight collagen peptide is about 500 to 2,000 Da. It means a peptide having a molecular weight.
또한, 본 명세서에서 특별히 언급하지 않는 한 %는 중량%를 의미한다. In addition, unless otherwise specified in the specification,% means% by weight.
1. 저분자 콜라겐 펩타이드의 제조 및 산업화1. Manufacturing and industrialization of small molecule collagen peptides
저분자 콜라겐 펩타이드 제조를 위하여 홍어 가공시 발생되는 부산물인 껍질을 이용하여 분자량 별로 콜라겐 펩타이드를 추출하였으며, 이를 이용한 산업적 활용 가능성을 확인하기 위한 실험으로 일반성분 및 분자량, 아미노산 조성, 탈취 등의 실험을 통해 산업적 적용 가능성을 검토하였다.For the production of low molecular weight collagen peptides, collagen peptides were extracted by molecular weight by using the skin, a by-product generated during processing of skates, and this is an experiment to confirm the industrial applicability. Through experiments such as general ingredients, molecular weight, amino acid composition, and deodorization. The industrial applicability was reviewed.
가. 재료 및 방법end. Materials and methods
(1) 홍어 껍질 원료의 가수분해(1) Hydrolysis of raw materials of skate skin
도 1 및 도 2에 보는 바와 같이, 홍어 껍질 원료의 가수분해를 위해, 우선적으로 자사(영산홍어(주))의 홍어가공 공정 중 껍질 탈피 공정 중에 발생된 홍어껍질 원재료(홍어 껍질 100%로 이루어짐)를 수집하였다(S10). As shown in Figs. 1 and 2, for hydrolysis of the raw materials of skate skin, the raw material of the skate skin generated during the peeling process during the process of processing the skates of our company (Youngsan Skate Co., Ltd.) (consists of 100% skate skin) ) Was collected (S10).
이후, 흐르는 물에 3회 수세 후 -25℃ 이하에서 냉동보관하였다(S20). Then, after washing with running water three times, it was stored frozen at -25 ℃ or less (S20).
저분자 콜라겐 펩타이드 제조를 위하여 껍질을 고압반응기(autoclave)에 넣고 121℃에서 90분간 동안 고압열수추출하였고(S30), 그 다음으로 상업적 단백질 가수분해 효소 Alcalase, Flavourzyme 및 Protamex(Novozyme, Denmark)를 이용하여 조건별로 가수분해 하였다(S40).To prepare the low molecular weight collagen peptide, the skin was put in a high-pressure reactor (autoclave) and extracted with high-pressure hot water at 121°C for 90 minutes (S30), and then commercial proteolytic enzymes Alcalase, Flavourzyme, and Protamex (Novozyme, Denmark) were used. Hydrolyzed for each condition (S40).
(2) 분자량별 콜라겐 펩타이드 제조(2) Preparation of collagen peptides by molecular weight
가수분해가 완료된 후, 활성탄 및 이온교환수지를 이용하여 어취 제거를 위한 탈취 공정을 거친 후(S50), 감압여과를 통해 불순물을 제거하였다(S60). 여과를 거친 액은 한외여과장치를 이용하여 분자량별로 분획하거나, 효소 종류 및 가수분해 시간에 따라 분자량별로 분획하는 두 가지 방법을 통해 분자량 1,000Da.을 기준으로 cut-off를 실시하였다. 분자량 cut-off를 거친 액은 농축을 거쳐(S70) 동결건조(S70)(SFDSM06, Samwon Co,. Korea)하여 실험에 사용하였으며, 산업적 생산을 분무건조기를 이용하여 제품화하였다.After the hydrolysis was completed, after going through a deodorizing process for removing fish odor using activated carbon and an ion exchange resin (S50), impurities were removed through vacuum filtration (S60). The filtered liquid was cut-off based on a molecular weight of 1,000 Da. by fractionating by molecular weight using an ultrafiltration device or by fractionating by molecular weight according to the enzyme type and hydrolysis time. The liquid that passed the molecular weight cut-off was concentrated (S70), lyophilized (S70) (SFDSM06, Samwon Co,. Korea), and used in the experiment, and industrial production was commercialized using a spray dryer.
나. 이화학적 특성 측정I. Measurement of physicochemical properties
(1) 일반성분 측정(1) Measurement of general ingredients
일반성분은 식품공전(식품공전, 2013)의 일반시험법에 준하여 측정하였다. 수분함량은 상압가열건조법, 조단백함량은 micro kjeldahl법, 조지방 함량은 Soxhlet법, 회분 함량은 직접회화법으로 측정하였으며, 각 측정은 세 번 반복하여 그 평균을 취하였다. General ingredients were measured according to the general test method of the Food Code (Food Code, 2013). Moisture content was measured by normal pressure drying method, crude protein content was measured by micro kjeldahl method, crude fat content was measured by Soxhlet method, and ash content was measured by direct painting method. Each measurement was repeated three times to obtain the average.
(2) 아미노산 분석(2) amino acid analysis
건조된 시료 일정량에 염산을 가하고 가수분해한 다음에 질소가스를 치환하여, 110℃의 오븐에서 22시간 정치 후, 감압 증발 하여 건조하였다. 여기에 0.02N 염산으로 희석한 후, 0.45μm filter로 여과하여 일정량을 취하여 아미노산 자동분석기(L-8800 Hitachi Co,. Japan)를 이용하여 분석하였다. Hydrochloric acid was added to a certain amount of the dried sample, followed by hydrolysis, nitrogen gas was substituted, and left to stand in an oven at 110° C. for 22 hours, followed by evaporation under reduced pressure and dried. It was diluted with 0.02N hydrochloric acid, filtered through a 0.45 μm filter, and a certain amount was taken and analyzed using an automatic amino acid analyzer (L-8800 Hitachi Co,. Japan).
다. 분자량 측정All. Molecular weight measurement
콜라겐 펩타이드의 분자량은 GPC(Gel Permeation Chromatography, Japan)를 이용하여 측정하였으며, 표준품은 PEG calibration kit(agilent technologies, United Kingdom)를 사용하였다. 이때 컬럼은 TSKgel SuperMultipore®PW-H(6.0 mm I.D. Х 15 cm, Distilled Water, Japan)을 이용하였으며, 시료 2㎕를 주입하여 1mL/min의 속도로 pH7.0의 buffer (0.1M KH2PO4 + 10% NaOH)를 흘려주면서 25℃에서 측정하였다.The molecular weight of the collagen peptide was measured using GPC (Gel Permeation Chromatography, Japan), and the standard product was a PEG calibration kit (agilent technologies, United Kingdom). At this time, TSKgel SuperMultipore®PW-H (6.0 mm ID Х 15 cm, Distilled Water, Japan) was used as the column, and 2 µl of the sample was injected into a buffer of pH 7.0 at a rate of 1 mL/min (0.1M KH2PO4 + 10%). NaOH) was measured at 25°C while flowing.
라. 한외여과la. Ultrafiltration
홍어껍질로부터 추출한 추출액을 한외여과기(EMP Series, EMP Tech, Korea)를 이용하여 Flow20으로 1K Da. 여과막(Pall corporation, USA)을 통과시켰다. 더 이상 여과액이 나오지 않을 때 까지 여과를 시행하였으며, 여과가 끝난 후 여과된 액과 여과되지 않은 액을 구분하여 시료를 제조하였다.Using an ultrafilter (EMP Series, EMP Tech, Korea), the extract extracted from the skate shell was used as Flow20 to 1K Da. It was passed through a filtration membrane (Pall Corporation, USA). Filtration was performed until no more filtrate came out, and a sample was prepared by separating the filtered liquid and the unfiltered liquid after the filtration was finished.
마. 인체적용시험hemp. Human body application test
인체적용시험은 임상시험 모니터링 계획 수립 후, 임상심의 위원회의 IRB 승인을 거쳐 수행하였으며, 헬싱키 선언을 준수하였고, 의약품 임상시험 관리 기준(KGCP)을 준용하여 수행하였다. Human application trials were conducted after establishing a clinical trial monitoring plan, approved by the Institutional Review Board, and approved by the Institutional Review Board, followed the Declaration of Helsinki, and followed the Pharmaceutical Clinical Trial Management Standard (KGCP).
대상자를 선정하기 위한 Screening 시행 전에 모든 지원자를 대상으로 본 시험에 대한 설명문을 배포하였고, 충분히 설명한 후 지원자 본인의 자발적 의사에 의하여 동의서를 자필 서명하였고, 그 사본을 각 대상자들에게 제공하였다.Before screening to select subjects, explanations for this test were distributed to all applicants, and after sufficient explanations, consent was signed by the applicant's own voluntary intention, and a copy was provided to each subject.
바. 인체 적용 시험을 위한 캡슐 제조bar. Capsule manufacturing for human application testing
인체 적용 시험을 위한 캡슐의 제조는 건강기능식품 전문 제조회사에서 표 1의 배합비로 제조하였다. 홍어 콜라겐 펩타이드 99%와 캡슐 충진을 원활하게 하기 위하여 체내 흡수 시 대사 작용을 일으키지 않는 안전한 식품 첨가물인 이산화규소를 사용하였다. 또한, 플라세보는 100% 전분을 원료로 제조하였다. The preparation of capsules for human application test was prepared in the blending ratio of Table 1 by a health functional food manufacturer. In order to smoothly fill the capsule with 99% of skate collagen peptide, silicon dioxide, a safe food additive that does not cause metabolism during absorption in the body, was used. In addition, placebo was prepared from 100% starch as a raw material.
[표 1] 캡슐 원료 배합비[Table 1] Blending ratio of raw materials for capsules
[표 2] 인체 적용 시험용 식품의 정보[Table 2] Information on food for human application test
2. 결과 및 고찰2. Results and Discussion
가. 이화학적 특성end. Physicochemical properties
(1) 일반성분(1) General ingredients
홍어껍질 콜라겐 펩타이드의 일반성분 함량은 표 3에 나타내었다. 콜라겐 펩타이드는 단백질의 함량이 약 95%, 지방함량은 약 0.1%, 회분은 약 1.2%, 탄수화물은 0%로 나타났다. 이러한 결과는 숙성홍어회 제조 과정 중 탈피과정에서 껍질과 홍어 육이 완전히 분리되었으며, 홍어 껍질 중에 대부분의 성분이 단백질로 구성되어 있기 때문으로 판단되었다. The content of general components of the skate skin collagen peptide is shown in Table 3. Collagen peptides were found to have about 95% protein, about 0.1% fat, about 1.2% ash, and 0% carbohydrates. This result was judged to be because the skin and skate meat were completely separated during the peeling process during the manufacturing process of aged skate sashimi, and most of the ingredients in the skate skin were composed of protein.
[표 3] The result of proximate analysis in collagen peptide of skate skin[Table 3] The result of proximate analysis in collagen peptide of skate skin
(2) 구성 아미노산(2) constituent amino acids
표 4에는 홍어 껍질로부터 제조한 저분자 콜라겐 펩타이드의 구성 아미노산 함량 분석결과를 나타낸 것이다. S40 공정시, 가수분해 효소로서 Alcalae 0.1~0.5 중량%와 Flavourzyme 0.1~0.5 중량%를 동시에 첨가하여 가수분해하거나, Alcalae 0.1~0.5 중량%와 Protamex 0.1~0.5 중량%를 동시에 첨가하여 가수분해함으로써 저분자 콜라겐 펩타이드를 제조한 결과이다. 표 4에 수록된 바와 같이, 구성 아미노산의 측정 결과 분자량에 관계없이 glycine 함량이 가장 높게 나타났으며, 다음으로 glutamic acid, alanine, arginine 순으로 함량이 높게 나타났다. 분자량과 아미노산 조성은 큰 차이를 나타나지 않았으나, 분자량이 낮을수록 아미노산 함량이 높게 측정되었다.Table 4 shows the results of analysis of the amino acid content of the low-molecular collagen peptide prepared from skate skin. In the S40 process, as a hydrolytic enzyme, 0.1-0.5% by weight of Alcalae and 0.1-0.5% by weight of Flavourzyme are added at the same time to hydrolyze, or 0.1-0.5% by weight of Alcalae and 0.1-0.5% by weight of Protamex are simultaneously added and hydrolyzed. This is the result of preparing a collagen peptide. As shown in Table 4, as a result of measuring the constituent amino acids, the glycine content was the highest regardless of the molecular weight, followed by glutamic acid, alanine, and arginine in order. The molecular weight and amino acid composition did not show a significant difference, but the lower the molecular weight, the higher the amino acid content was measured.
[표 4] Analysis of constituent amino acid with molecular weights of collagen peptide[Table 4] Analysis of constituent amino acid with molecular weights of collagen peptide
(3) 유리 아미노산(3) free amino acids
표 5에는 홍어 껍질로부터 제조한 저분자 콜라겐 펩타이드의 유리 아미노산 함량 분석결과를 나타낸 것이다. 유리 아미노산의 결과 glycine, hydroxy proline의 함량이 약 22.0%, 7.0%로 전형적인 콜라겐 단백질의 특성을 나타내었으며, 분자량에 따른 아미노산 조성은 큰 차이를 나타내지 않았으며, 구성아미노산과 마찬가지로 분자량이 작을수록 아미노산의 함량이 높게 나타났다.Table 5 shows the results of analysis of free amino acid content of low molecular weight collagen peptides prepared from skate skin. As a result of free amino acids, the contents of glycine and hydroxy proline were about 22.0% and 7.0%, indicating the characteristics of a typical collagen protein, and the amino acid composition according to the molecular weight did not show a big difference. The content was high.
[표 5] Analysis of free amino acids in skate skin collagen peptide[Table 5] Analysis of free amino acids in skate skin collagen peptide
(4) 지표성분(또는 기능성분) 규격 및 시험방법에 관한 자료(4) Data on the standards and test methods for indicator components (or functional components)
- 지표성분(또는 기능성분)의 규격 및 근거 -Standards and grounds for indicator components (or functional components)
○ 홍어 껍질 콜라겐펩타이드의 지표성분은 단백질 함량 90% 이상, Hydroxy proline 7% 이상, Glycin 20% 이상의 3종으로 설정하였다. ○ The index components of skate skin collagen peptide were set to be 90% or more of protein content, 7% or more of hydroxy proline, and 20% or more of Glycin.
[표 6] 홍어 껍질 콜라겐 펩타이드 추출물에 대한 지표성분 분석결과[Table 6] Analysis results of index components for collagen peptide extract from skate skin
○ 지표성분 함량의 규격은 공인 기관에서 분석된 결과에 대한 80~120% 범위로 설정하는 것이 바람직하다. ○ It is desirable to set the standard for the content of indicator components in the range of 80 to 120% of the results analyzed by an accredited institution.
[표 7] 홍어 껍질 콜라겐 펩타이드 추출물에 대한 지표성분 분석결과[Table 7] Analysis results of index components for the collagen peptide extract of skate skin
- 지표성분(또는 기능성분) 표준품 정보-Indicator component (or functional component) standard product information
○ Hydroxy proline○ Hydroxy proline
○ Glycine○ Glycine
- 지표성분 설정근거-Grounds for setting indicator components
○ Glycine : 공인 시험기관 성적서 요약표○ Glycine: Summary table of the report of an accredited testing institution
○ Hydroxy proline : 공인 시험기관 성적서 요약표○ Hydroxy proline: Summary table of official test institute report
■ 시험성적서 요약표■ Summary table of test report
나. 홍어껍질(Skate Skin)유래 저분자 콜라겐 펩타이드의 경구 섭취가 체지방 감소에 미치는 영향I. The Effect of Oral Intake of Skate Skin-derived Low Molecular Collagen Peptide on Body Fat Reduction
홍어껍질로부터 추출하여 제조한 콜라겐 펩타이드의 경구 섭취가 체지방 감소에 미치는 영향을 알아보기 위해 본 인체시험 대상자에 대한 시험용/대조군 식품 투여 전의 기저 특성에 군 간 차이가 있는지 검정하였다. 표 8에 수록된 바와 같이, 그 결과 ITT군 및 PP군 모두 성별, 나이, 체중, 신장 등에 통계적으로 유의한 차이는 관측되지 않아 두 군의 사전 동질성이 인정된다고 볼 수 있고, 무작위 배정은 통계학적으로 적절하다고 판단된다.In order to investigate the effect of oral intake of collagen peptides extracted from skate shells on body fat reduction, we tested whether there was a difference between groups in the basal characteristics before administration of the test/control food for this human test subject. As shown in Table 8, as a result, there was no statistically significant difference in sex, age, weight, height, etc. in both the ITT group and the PP group, so it can be seen that prior homogeneity of the two groups is recognized, and randomization is statistically appropriate. I think it is.
[표 8] Study patients baseline characteristics[Table 8] Study patients baseline characteristics
표 9 및 표 10에 수록한 바와 같이, 1차 유효성 평가변수인 DEXA를 통한 체지방률(%)의 변화와 2차 유효성 평가변수에 대하여 시험용/대조군 식품의 투여 전(Visit2, week0)과 투여 후인 Visit3(week6) 및 Visit4(week12)에서 측정하였다. 각 측정값과 baseline(Visit2) 대비 변화정도에 대하여 군 간과 군 내(방문시기별)의 값도 함께 비교하여 표로 나타내었다.As listed in Tables 9 and 10, for the change in body fat percentage (%) through DEXA, the primary efficacy endpoint, and Visit3 before and after administration of the test/control food (Visit2, week0) for the secondary efficacy endpoint. It was measured at (week6) and Visit4 (week12). For each measurement value and the degree of change compared to the baseline (Visit2), the values between groups and within groups (by visit time) were also compared and shown in a table.
[표 9] Comparison between and within each groups (ITT population)[Table 9] Comparison between and within each groups (ITT population)
[표 10] Comparison between and within each groups (PP population)[Table 10] Comparison between and within each groups (PP population)
표 9 및 표 10에 보는 바와 같이, 1차 유효성 평가변수 분석 결과 ITT 분석군과 PP 분석군 모두 DEXA로 측정한 체지방률(%) 변화량에 대한 군 내 비교에서 시험군에서는 유의한 감소를 보였고, 시험군의 변화량을 보였다. 따라서 시험식품의 일상적인 복용이 체지방 감소 개선에 도움을 주는 것으로 사료된다. As shown in Table 9 and Table 10, the results of the primary efficacy evaluation variable analysis showed a significant decrease in the test group in the comparison of the change in body fat percentage (%) measured by DEXA in both the ITT and PP analysis groups. It showed the amount of change in the group. Therefore, it is believed that the daily use of test foods helps improve body fat reduction.
2차 유효성 평가변수 분석 결과에서도 DEXA로 측정한 체지방량(g) 변화량에 대한 군 간 비교에서 시험군이 대조군보다 유의하게 감소하였다. In the results of the secondary efficacy endpoint analysis, the test group significantly decreased compared to the control group in the comparison of the change in body fat mass (g) measured by DEXA.
또한 각 군 내 전/후 비교에서 시험군에서 감소하는 경향을 보였고 시험군에서의 변화량을 나타내어 임상적으로 유의한 것으로 사료된다. In addition, in the before/after comparison within each group, there was a tendency to decrease in the test group and the amount of change in the test group was considered to be clinically significant.
체지방 감소의 평가지표로 설정한 DEXA를 통해 체지방량(g)분석 결과 시험군에서 대조군에 비해 유의하게 감소하였고, 그 감소폭도 임상적으로 유의하다고 판단되므로 시험 식품(홍어껍질 유래의 콜라겐 펩타이드)은 체지방 감소에 도움을 준다고 사료된다. As a result of analysis of body fat mass (g) through DEXA, which was set as an evaluation index for body fat reduction, the test group significantly decreased compared to the control group, and the extent of the reduction was also judged to be clinically significant, so the test food (collagen peptide derived from skate skin) was It is thought that it helps to reduce.
따라서 본 시험 식품은 중·장기적으로 복용하였을 때 체지방 감소에 효과를 줄 수 있을 것이라고 판단된다. Therefore, it is judged that this test food can have an effect on reducing body fat when taken in the medium and long term.
한편, 안전성 평가로 시험용 식품을 1회 이상 복용한 시험군 45명과 대조군 45명의 대상자에 대해 시험 기간 중에 생체징후 평가, 임상실험실검사 등으로 안전성을 평가하였다. On the other hand, as a safety evaluation, 45 subjects in the test group and 45 subjects in the control group who took the test food at least once were evaluated for safety by evaluating vital signs and clinical laboratory tests during the test period.
표 11에 보는 바와 같이, 시험 기간 중 이상반응이 발생한 사례가 1건(소화불량) 있었으나 보고된 이상반응은 해소되었으며, 시험용 식품과의 연관성은 없는 것으로 판단되었다. 임상실험실 검사치 및 신체검사 등을 통한 안전성 평가결과 임상적으로 위해하다고 판단할 근거는 없었다. 따라서 본 홍어껍질 유래의 콜라겐 펩타이드를 성인 기준 2,000mg/day을 중·장기간 동안 섭취하여도 안전하다고 판단된다.As shown in Table 11, there was one case (dyspepsia) in which an adverse reaction occurred during the test period, but the reported adverse reaction was resolved, and it was judged that there was no association with the test food. As a result of safety evaluation through clinical laboratory test values and physical examination, there was no basis for judging that it was clinically hazardous. Therefore, it is judged that it is safe to ingest 2,000mg/day of the collagen peptide derived from the skate skin for an adult standard for a medium or long period.
[표 11] Atypical response report[Table 11] Atypical response report
다. 콜라겐 펩타이드의 대량 생산 및 관련 응용제품 생산All. Mass production of collagen peptides and production of related applications
(1) 콜라겐 펩타이드의 대량 생산(1) Mass production of collagen peptides
실험실상에서 최적화 된 조건을 이용하여 대량 생산 설비를 공장 현장에서 대량생산을 실시하였다. 숙성 홍어회 가공 중에 발생한 홍어 껍질 원료를 흐르는 물에서 수세하여 전처리를 하고, 120℃에서 2시간 동안 가압 열처리를 하여 원료를 젤라틴 겔화 시킨 다음, 분자량 1,000Da. 이상의 경우에는 온도에서 50℃에서 Alcalase와 Flavourzyme을 투입원료 대비 각각 0.1%(w/w)를 투입하여 2시간 동안 효소가수분해 하였고, 분자량 1.000Da. 이하의 제품은 Alcalase와 Flavourzyme을 투입원료 대비 각각 0.2%(w/w)를 투입하여 6시간 동안 효소가수분해 하였다. 가수분해 종료 후, 1차 여과를 통하여 잔사를 제거하고, 탈취를 위하여 활성탄을 투입 후, 75℃에서 30분간 교반하였다. 탈취 종료 후, 활성탄 제거를 위하여 필터프레스를 이용하여 정밀여과를 거쳐서 진공농축기에서 30brix까지 농축하여 액중의 고형분 함량의 농도를 높인 후 스프레이 건조하여 제품화 하였다. 제품의 수율은 약 17% 정도로 나타났는데, 홍어 껍질 원료의 수분 함량을 고려하였을 때 상당히 높은 수율을 나타내었다. 이를 토대로 하여 제품 표준서를 작성하였다. Mass production facilities were mass-produced at the factory site using conditions optimized in the laboratory. The raw materials of skate skins generated during the processing of aged skates are pretreated by washing them with running water, and subjected to pressure heat treatment at 120°C for 2 hours to gelatinize the raw materials, and then have a molecular weight of 1,000 Da. In the above case, 0.1% (w/w) of Alcalase and Flavourzyme were added to each of the raw materials at 50° C., and the enzyme was hydrolyzed for 2 hours, and the molecular weight was 1.000 Da. The following products were enzymatically hydrolyzed for 6 hours by adding 0.2% (w/w) of Alcalase and Flavourzyme respectively to the input raw materials. After completion of hydrolysis, the residue was removed through primary filtration, and activated carbon was added for deodorization, followed by stirring at 75° C. for 30 minutes. After the deodorization was finished, it was concentrated to 30 brix in a vacuum concentrator through microfiltration using a filter press to remove activated carbon, and then increased the concentration of the solid content in the liquid, and spray dried to commercialize it. The yield of the product was about 17%, which was considerably high when considering the moisture content of the raw materials of the skate skin. Based on this, a product standard was prepared.
(2) 응용 제품 생산(2) Application product production
가. 저분자 콜라겐 펩타이드 분말 제품end. Low molecular weight collagen peptide powder product
현장에서 콜라겐 펩타이드를 대량생산하였으며, 대량생산된 제품은 100g 단위로 포장한 제품을 출시 및 판매하였다Collagen peptides were mass-produced on-site, and mass-produced products were released and sold in units of 100g.
나. 인체시험용 홍어 콜라겐 펩타이드 캡슐 제품I. Skate Collagen Peptide Capsule Product for Human Test
인체시험을 위하여 홍오 콜라겐 펩타이드 100%를 충진한 캡슐 제품을 제작하였으며, 대조구 시험을 위한 플라세보 제품도 동량 제작하였다.For the human test, a capsule product filled with 100% of Hongo collagen peptide was produced, and the same amount of placebo product for the control test was produced.
다. 홍어 콜라겐 펩타이드(건강기능식품) 캡슐 제품All. Skate Collagen Peptide (Health Functional Food) Capsule Product
홍어 콜라겐 펩타이드를 주원료로 한 건강기능식품을 제작하였다. 비타민 1.7%, 콜라겐 펩타이드 70%의 배합비로 식약청 인허가를 거쳐 캡슐형의 건강기능식품으로 제조하였다.A health functional food was produced using skate collagen peptide as the main ingredient. It was manufactured as a capsule-type health functional food after obtaining approval from the Food and Drug Administration at a blending ratio of 1.7% vitamin and 70% collagen peptide.
본 명세서에서 설명되는 실시예와 첨부된 도면은 본 발명에 포함되는 기술적 사상의 일부를 예시적으로 설명하는 것에 불과하다. 따라서, 본 명세서에 개시된 실시예들은 본 발명의 기술적 사상을 한정하기 위한 것이 아니라 설명하기 위한 것이므로, 이러한 실시예에 의하여 본 발명의 기술 사상의 범위가 한정되는 것이 아님은 자명하다. 본 발명의 명세서 및 도면에 포함된 기술적 사상의 범위 내에서 당해 기술분야에 있어서의 통상의 지식을 가진 자가 용이하게 유추할 수 있는 변형예와 구체적인 실시예는 모두 본 발명의 권리범위에 포함되는 것으로 해석되어야 할 것이다.The embodiments described in the present specification and the accompanying drawings are merely illustrative of some of the technical ideas included in the present invention. Accordingly, it is obvious that the embodiments disclosed in the present specification are not intended to limit the technical idea of the present disclosure, but to explain the technical idea, and thus the scope of the technical idea of the present disclosure is not limited by these embodiments. Modifications and specific embodiments that can be easily inferred by those of ordinary skill in the art within the scope of the technical idea included in the specification and drawings of the present invention are included in the scope of the present invention. It will have to be interpreted.
Claims (5)
단백질 함량이 90 중량% 이상이고, 하기 화학식 1로 표현되는 제1 화합물 58~89 mg/g 및 하기 화학식 2로 표현되는 제2 화합물 190~286 mg/g 을 함유한 저분자 콜라겐 펩타이드를 유효성분으로 포함하되,
상기 저분자 콜라겐 펩타이드는
홍어가공 공정 중에 발생된 홍어 껍질 원재료(홍어 껍질 100%로 이루어짐)를 수집하여 냉동보관하는 단계;
껍질을 고압반응기에 넣고 121℃에서 90분간 고압열수추출하는 단계;
단백질 가수분해 효소인 Alcalase와 Protamax을 투입원료 대비 각각 0.5%를 2시간 동안 투입하여 가수분해하는 단계;
활성탄 및 이온교환수지를 이용하여 탈취하는 단계;
감압여과를 통해 불순물을 제거하는 단계; 및
분자량 1,000Da.를 기준으로 컷오프한 후 농축 및 동결건조하는 단계; 를 거쳐 제조되며,
상기 저분자 콜라겐 펩타이드의 유리 아미노산은, 상기 유리 아미노산 전체 100 중량%을 기준으로 하여 상기 제1 화합물 6~8 중량% 및 상기 제2 화합물 19~23 중량%를 포함하며,
상기 저분자 콜라겐 펩타이드의 구성 아미노산은 상기 구성 아미노산 전체 100 중량%를 기준으로 Aaspartic acid 5.36~5.90 중량%, Threonine 3.38~3.72 중량%, Serine 9.77~10.71 중량%, Glutamic acid 9.77~10.71 중량%, Glycine 21.27~23.64 중량%, Alanine 7.65~8.51 중량%, Valine 2.88~3.17 중량%, Methionine 1.72~2.16 중량%, Isoleucine 2.34~2.58 중량%, Leucine 3.50~3.87 중량%, Tyrosine 0.73~0.86 중량%, Phenylalanine 2.07~2.28 중량%, Lysine 3.54~3.88 중량%, Ammonia 0.84~0.92 중량%, Histidine 1.49~1.62 중량% 및 Arginine 7.11~7.93 중량%을 포함하는 것을 특징으로 하는 체지방 감소 기능성 원료 조성물.
[화학식 1]
[화학식 2]
As a functional raw material composition for reducing body fat comprising a low molecular weight collagen peptide obtained by hydrolyzing skate skin as an active ingredient,
As an active ingredient, a low-molecular collagen peptide containing a protein content of 90% by weight or more and containing 58-89 mg/g of the first compound represented by the following Formula 1 and 190-286 mg/g of the second compound represented by the following Formula 2. Including,
The low molecular weight collagen peptide is
Collecting raw materials of skate skin (consisting of 100% skate skin) generated during the skate processing process and storing them in a frozen manner;
Putting the skin in a high-pressure reactor and extracting hot water at 121° C. for 90 minutes;
Hydrolyzing the proteolytic enzymes Alcalase and Protamax by adding 0.5% of each of the raw materials for 2 hours;
Deodorizing using activated carbon and ion exchange resin;
Removing impurities through vacuum filtration; And
Concentrating and freeze-drying after cut-off based on molecular weight of 1,000 Da.; It is manufactured through
The free amino acids of the low molecular weight collagen peptide include 6 to 8% by weight of the first compound and 19 to 23% by weight of the second compound, based on 100% by weight of the total free amino acids,
The constituent amino acids of the low molecular weight collagen peptide are Aaspartic acid 5.36 to 5.90 wt%, Threonine 3.38 to 3.72 wt%, Serine 9.77 to 10.71 wt%, Glutamic acid 9.77 to 10.71 wt%, Glycine 21.27 based on 100 wt% of the total amino acids. ~23.64 wt%, Alanine 7.65~8.51 wt%, Valine 2.88~3.17 wt%, Methionine 1.72~2.16 wt%, Isoleucine 2.34~2.58 wt%, Leucine 3.50~3.87 wt%, Tyrosine 0.73~0.86 wt%, Phenylalanine 2.07~ 2.28 wt%, Lysine 3.54 to 3.88 wt%, Ammonia 0.84 to 0.92 wt%, Histidine 1.49 to 1.62 wt%, and Arginine 7.11 to 7.93 wt% A functional raw material composition for reducing body fat, characterized in that it comprises.
[Formula 1]
[Formula 2]
상기 저분자 콜라겐 펩타이드의 유리 아미노산은, 상기 유리 아미노산 전체 100 중량%를 기준으로 Phosphoserine 0.03~0.05 중량%, Taurine 0.01~0.02 중량%, Aspartic acid 6.46~7.11 중량%, Hydroxy proline 6.10~6.85 중량%, Threonine 3.29~3.42 중량%, Serine 5.38~5.71 중량%, Glutamic acid 9.93~10.78 중량%, Proline 8.28~9.02 중량%, Glycine 19.97~22.09 중량%, Alanine 6.92~7.66 중량%, Valine 2.93~3.34 중량%, Methionine 1.67~2.15 중량%, Isoleucine 2.20~2.45 중량%, Leucine 3.32~3.67 중량%, Tyrosine 0.81~0.91 중량%, Phenylalanine 2.02~2.23 중량%, Ammonia 0.71~0.74 중량%, Hyroxylysine 0.09~1.05 중량%, Ornithine 0.03~0.04 중량%, Lysine 3.22~3.49 중량%, Histidine 1.26~1.38 중량% 및 Arginine 7.05~7.84 중량%를 포함하는, 체지방 감소 기능성 원료 조성물.
The method of claim 1,
The free amino acid of the low molecular weight collagen peptide is 0.03 to 0.05% by weight of Phosphoserine, 0.01 to 0.02% by weight of Taurine, 6.46 to 7.11% by weight of Aspartic acid, 6.10 to 6.85% by weight of Hydroxy proline, Threonine 3.29~3.42 wt%, Serine 5.38~5.71 wt%, Glutamic acid 9.93~10.78 wt%, Proline 8.28~9.02 wt%, Glycine 19.97~22.09 wt%, Alanine 6.92~7.66 wt%, Valine 2.93~3.34 wt%, Methionine 1.67~2.15 wt%, Isoleucine 2.20~2.45 wt%, Leucine 3.32~3.67 wt%, Tyrosine 0.81~0.91 wt%, Phenylalanine 2.02~2.23 wt%, Ammonia 0.71~0.74 wt%, Hyroxylysine 0.09~1.05 wt%, Ornithine 0.03 -0.04% by weight, Lysine 3.22 to 3.49% by weight, Histidine 1.26 to 1.38% by weight, and Arginine 7.05 to 7.84% by weight, comprising a body fat reduction functional raw material composition.
Functional food comprising the functional raw material composition for reducing body fat according to any one of claims 1 and 4.
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