KR20210135046A - Cosmetic composition for skin improvement containing aloe vera adventitious root extract - Google Patents

Abstract

The present invention relates to a cosmetic composition for skin improvement which contains an Aloe vera adventitious root extract as an active ingredient of a cosmetic composition having skin wrinkle improvement and whitening effects to ensure excellent anti-inflammatory and antioxidant activity, has an excellent effect of promoting the production of procollagen, and can inhibit production of melanin pigment and matrix metalloproteinase; and a method for preparing the cosmetic composition.

Description

Cosmetic composition for skin improvement containing aloe vera adventitious root extract}

The present invention relates to a cosmetic composition for improving skin containing an Aloe vera root extract and a method for preparing the cosmetic composition.

As the aging population increases, skin beauty, which can be a measure of aging, and antioxidants that can prevent aging are emerging as major concerns. Such aging is continuously exposed to oxidative stress, which increases free radicals in the body, increases the activity of elastase that decomposes elastin, the connective tissue of the dermis, and hyaluronic acid ( This can be explained by the increased activity of hyaluronidase that degrades hyaluronic acid. In addition, free radicals in the body are involved in the formation of melanin and activate tyrosinase, which may cause spots and freckles.

Therefore, mechanistic studies on skin health and antioxidants have been actively conducted, and with the development of the research level and increase in technology, a number of studies to develop materials with biochemical efficacy and effects are in progress. In particular, plant-derived materials have been used for a long time in recognition of their excellent safety in terms of safety.

Among plant-derived materials, Aloe vera is native to the Mediterranean region and Africa. It is a bushy perennial herb that is used for various purposes such as cosmetics, raw materials for pharmaceuticals, food, etc. It has been reported to be effective against fever, constipation and ulcers.

On the other hand, Korean Patent No. 10-1822092 discloses 'a cosmetic composition for preventing heat aging containing a complex extract of aloe vera leaf', and Korean Patent No. 10-1477959 discloses 'a skin containing aloe extract as an active ingredient'. Although the 'anti-irritant cosmetic composition' has been disclosed, there has been no disclosure of a cosmetic composition for skin wrinkle improvement and whitening containing an Aloe vera root extract as an active ingredient.

An object of the present invention is to provide a cosmetic composition for skin wrinkle improvement and whitening containing an Aloe vera root extract and a method for preparing the cosmetic composition.

In particular, the present invention relates to the 'mass propagation method of aloe disclosed in Korean Patent No. 0133344' and the 'method for producing aloe irregular root with increased anthraquinone compound content' disclosed in Korean Patent No. 10-1576787 and the method. using the aloe irregular root produced by plant tissue culture technology based on the 'method for producing aloe irregular root with increased pharmacologically active substances' disclosed in Korean Patent No. 10-1294742, It was confirmed that the extract of Aloe vera root was significantly superior to the Aloe vera extract in inhibiting nitric oxide (NO) production and scavenging free radicals, as well as inhibiting the production of melanin and matrix metalloprotease (MMP). want to

In order to solve the above problem,

In one embodiment, the present invention contains an Aloe vera root extract as an active ingredient of a cosmetic composition having skin wrinkle improvement and whitening effects, and the Aloe vera root extract is 1 to 5% by weight based on the total weight of the cosmetic composition. It provides a cosmetic composition for skin improvement comprising a.

In addition, in one embodiment, the present invention comprises the steps of: preparing a mixture by supporting Aloe vera root in an organic solvent; leaving the mixture at room temperature to prepare an Aloe vera root extract; and preparing a cosmetic composition comprising the Aloe vera root extract.

The cosmetic composition according to the present invention contains an Aloe vera root extract, which has no cytotoxicity and has excellent anti-inflammatory and antioxidant activity. In addition, the Aloe vera root extract inhibits the production of melanin pigment and has the effect of enhancing the production of procollagen, a precursor of collagen.

1 is a photograph showing Aloe vera irregular root induced and propagated by tissue culture technology in an embodiment of the present invention.
2 is a result of confirming the cytotoxicity of Aloe vera or water or ethanol extract of Aloe vera root to mouse macrophages in an Example of the present invention: nor (untreated normal cells); Lipopolysaccharde (LPS); Mono-methyl-L-arginine (NMMA); indo (indomethacin).
3 is a result of analyzing the production level of nitric oxide (NO) by treating LPS-treated mouse macrophages with water or ethanol extract of Aloe vera or Aloe vera irregular root in an Example of the present invention. : nor (untreated normal cells); Lipopolysaccharde (LPS); Mono-methyl-L-arginine (NMMA); indo (indomethacin).
4 is an analysis of the change in the amount of matrix metalloprotease (MMP) production after treatment with water or ethanol extract of Aloe vera or Aloe vera irregular root to human fibroblasts irradiated with ultraviolet light in an embodiment of the present invention. As a result, A is the result for MMP-1 and B is the result for MMP-3.
5 is a result of analyzing the amount of procollagen produced after treating human fibroblasts irradiated with ultraviolet light with water or ethanol extract of Aloe vera or Aloe vera irregular root in an embodiment of the present invention.
6 is a result of confirming the cytotoxicity of Aloe vera or water or ethanol extract of Aloe vera root to mouse melanoma cells in an Example of the present invention: nor (untreated normal cells); KA (Kojic acid).
7 is a result of measuring the melanin level after treatment of Aloe vera or Aloe vera irregular root with water or ethanol extract to mouse melanoma cells in an Example of the present invention: nor (untreated normal cells); KA (Kojic acid).

Since the present invention can have various changes and can have various embodiments, specific embodiments are illustrated in the drawings and will be described in detail in the detailed description.

However, this is not intended to limit the present invention to specific embodiments, and it should be understood to include all modifications, equivalents and substitutes included in the spirit and scope of the present invention.

In the present invention, terms such as "comprises" or "have" are intended to designate that the features, numbers, steps, operations, components, parts, or combinations thereof described in the specification exist, but one or more other features It should be understood that this does not preclude the existence or addition of numbers, steps, operations, components, parts, or combinations thereof.

In the present invention, "Aloe vera" is native to the Mediterranean region and Africa, and means a bushy perennial. Stems are short, 30 to 60 cm high, and grow up to 100 cm tall. From this stem, many long, hard leaves come out in the form of rosettes. The leaves are 15-40 cm long and 2-7 cm wide, light green, thick, fleshy, and juicy. It is used for various purposes such as cosmetics and pharmaceutical raw materials, food, etc. It has been found to be effective in sterilization and toxin neutralization, fatigue recovery and skin neutralization, fever, constipation and ulcers. Vitamins A, C, D, E, B12, fatty acids, amino acids, selenium, calcium, and about 300 anthraquinones such as aloin, aloesin, and emodine contains ingredients. The distribution area is very wide, such as India, North Africa, and Manila.

In the present invention, the term " in vitro culture" is also referred to as in vitro culture, and refers to in vitro culture for reproducing a material taken out of an organism in vitro. In general, the types of plant hormones used for in-flight culture of plants include BAP (6-benzyloaminopurine), kinetin (Kinetin, N-2-furanylmethyl-1H-purine-6-amine), and TDZ (Thiazuron-N-phenyl-N). Cytokinins such as -1,2,3 thiadiazol-5ylurea); Auxins such as IAA (Indole acetic acid), IBA (Indole butyric acid), NAA (Naphthalene-acetic acid) and 2,4-D (2,4-dichlorophenoxy-acetic acid); and Gibberellins such as GA3. Plant hormones have different effective concentrations depending on the type of tissue to which they are applied, and it is often uncertain whether they will be transported to a place other than the place where they are synthesized and exhibit the same function. Therefore, it is important to appropriately select the type and concentration of the plant hormone according to the purpose.

In the present invention, the term "wrinkle improvement" refers to suppressing or inhibiting the generation of wrinkles on the skin, or alleviating the already generated wrinkles. In the present invention, wrinkle generation occurs due to a decrease in collagen, and the decrease in collagen may be caused by excessive oxidative stress or ultraviolet irradiation.

In the present invention, the term "whitening" means inhibiting, inhibiting or alleviating hyperpigmentation of the skin.

Hereinafter, the present invention will be described in detail.

The present invention contains an Aloe vera root extract as an active ingredient of a cosmetic composition having skin wrinkle improvement and whitening effects, and the Aloe vera root extract contains 1 to 5% by weight based on the total weight of the cosmetic composition. A cosmetic composition is provided.

The Aloe vera root extract according to the present invention is 1 to 4 wt%, 1 to 3 wt%, 1 to 2 wt%, 2 to 5 wt%, 3 to 5 wt%, or 4 to 5 wt% based on the total weight of the cosmetic composition % may be included.

The Aloe vera extract according to the present invention may be a root extract obtained from an Aloe vera tissue culture technique.

The Aloe vera irregular root may be an irregular root formed by the totipotency of a plant by removing a part of Aloe vera tissue and introducing it into an in vitro sterile state. The Aloe vera root is pre-cultured for a certain period of time and then mass-produced by trying the main culture in a bioreactor. Since harmful components such as diazinon, DDT, Metalaxyl) and heavy metals (eg, lead, cadmium) are fundamentally excluded, it has the advantage of being safe from pests and virus infection that may occur during the natural growth process.

The Aloe vera root extract according to the present invention is characterized in that it has an activity to promote the production of procollagen, a precursor of collagen, and to inhibit melanin and matrix metalloprotease (MMP). can The cosmetic composition for improving skin contains an extract of Aloe vera root, which has the effect of promoting the production of procollagen and inhibiting the production of melanin and matrix metalloproteinase (MMP) as an active ingredient, thereby improving skin wrinkles and whitening functions. have.

The Aloe vera root extract according to the present invention may be characterized as having anti-inflammatory and antioxidant activity. Specifically, the Aloe vera root extract may have an anti-inflammatory effect by inhibiting the production of nitric oxide (NO), and may have an antioxidant effect due to its free radical scavenging ability.

The cosmetic composition for improving skin according to the present invention includes skin, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nourishing lotion, massage cream, nourishing cream, eye cream, moisture cream, hand cream, essence, It may be characterized in that it is one or more formulations selected from the group consisting of nutritional essence, pack, cleansing foam, cleansing water, cleansing lotion, cleansing cream, body lotion, body cleanser, soap and powder. The cosmetic composition for skin improvement may contain various bases and additives necessary and appropriate for the formulation of the formulation, and these compositions and composition ratios may be appropriately selected according to the formulation.

When the formulation of the cosmetic composition according to the present invention is a paste, cream or gel, animal fiber, vegetable fiber, wax, paraffin, starch, tragacanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, It may include at least one selected from the group consisting of zinc oxide and combinations thereof.

When the formulation of the cosmetic composition according to the present invention is a solution or emulsion, a solvent, solvating agent or emulsifying agent may be included as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzo It may include one or more selected from the group consisting of ate, propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol, sorbitan fatty acid ester, and combinations thereof.

When the formulation of the cosmetic composition according to the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, such as suspension agent, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar, tragacanth, and may include one or more selected from the group consisting of combinations thereof.

When the formulation of the cosmetic composition according to the present invention is surfactant-containing cleansing, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, acetionate, imidazolinium derivative, methyl taurate, sarcocy nate, fatty acid amide ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, linolin derivative, ethoxylated glycerol fatty acid ester, and combinations thereof can

When the formulation of the cosmetic composition according to the present invention is a powder or a spray, one or more selected from the group consisting of lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder, and combinations thereof may be included as a carrier component. and, particularly in the case of a spray, may additionally contain a propellant such as chlorofluorohydrocarbon, propane-butane or dimethyl ether.

The cosmetic composition for improving skin containing the Aloe vera root extract according to the present invention is relatively inexpensive when commercialized as a wash-off type cosmetic such as a makeup remover, a detergent, etc., in which the active ingredient stays on the skin within a short period of time. High concentrations of Aloe vera root extract may be included. On the other hand, when the active ingredient is commercialized as a leave-on type cosmetic such as a lotion, emulsion, cream, or essence that stays on the skin for a long time, a lower concentration of aloe vera root extract compared to wash-off type cosmetics. may be included.

For example, when the cosmetic composition is a flexible lotion (skin) formulation, it may have a composition as shown in Table 1 below. In the table below, the trace amount may be 0.1 wt% or less, specifically 0.001 to 0.1 wt%.

ingredient content (wt%) Aloe Vera Root Ethanol Extract 1.0~3.0 glycerin 4.0~6.0 1.3-Butylene Glycol 2.0~3.0 PG 1500 1.0~0.2 allantoin 0.1~0.2 DL-Panthenol 0.2~0.4 EDTA-2Na 0.01~0.03 Benzophenone-9 0.03~0.05 Sodium Hyaluronate 4.0~6.0 ethanol 8.0~9.0 Octic dodeces-16 0.1~0.2 Polysorbate 20 0.1~0.2 preservatives, fragrances, colors a very small amount Distilled water remaining amount Sum 100

For example, when the cosmetic composition is a nutritional lotion (lotion) formulation, it may have a composition as shown in Table 2 below.

ingredient content (wt%) Aloe Vera Root Ethanol Extract 1.0~3.0 Glyceryl Stearate SE 1.0~2.0 stearyl alcohol 1.0~2.0 lanolin 1.0~2.0 Polysorbate 60 1.0~2.0 Sorbitan Stearate 0.4~0.6 hydrogenated vegetable oil 1.0~2.0 mineral oil 4.0~6.0 squalane 3.0~4.0 trioctanoin 1.0~2.0 dimethicone 0.8~1.2 tocopherol acetate 0.5~1.0 Carboxyvinyl Polymer 0.15 to 0.20 glycerin 4.0~6.0 1,3-butylene glycol 2.0~3.0 Sodium Hyaluronate 4.0~6.0 triethanolamine 0.15 to 0.20 preservatives, fragrances, colors a very small amount Distilled water remaining amount Sum 100

For example, when the cosmetic composition is an essence formulation, it may have a composition as shown in Table 3 below.

ingredient content (wt%) Aloe Vera Root Ethanol Extract 1.0~3.0 glycerin 9.0 to 11.0 betaine 4.0~6.0 Fiji 1500 1.0~3.0 allantoin 0.1~0.2 DL-Panthenol 0.2~0.4 EDTA-2Na 0.02~0.03 Benzophenone-9 0.03~0.04 Hydroxyethyl Cellulose 0.1~0.2 Sodium Hyaluronate 8.0 to 10.0 Carboxyvinyl Polymer 0.2~0.3 triethanolamine 0.10 to 0.30 Octyldodecanol 0.2~0.4 Octyldodeces-16 0.3~0.5 ethanol 5.0~7.0 preservatives, fragrances, colors a very small amount Distilled water remaining amount Sum 100

For example, when the cosmetic composition is a cream formulation, it may have a composition as shown in Table 4 below.

ingredient content content (wt%) Aloe Vera Root Ethanol Extract 1.0~3.0 lipophilic monostearate glycerin 1.0~3.0 cetearyl alcohol 1.0~3.0 stearic acid 1.2~1.5 beeswax 1.0~1.5 Polysorbate 60 0.1~0.2 Sorbitan Stearate 0.4~0.6 hydrogenated vegetable oil 1.0~2.0 squalane 3.0~4.0 mineral oil 4.0~6.0 trioctanoin 4.0~6.0 dimethicone 0.8~1.2 Sodium Magnesium Silicate 0.8~1.2 glycerin 4.0~6.0 betaine 2.0~4.0 triethanolamine 1.0~1.5 Sodium Hyaluronate 4.0~6.0 preservatives, fragrances, colors a very small amount Distilled water remaining amount Sum 100

In addition, the present invention comprises the steps of preparing a mixture by supporting Aloe vera root in an organic solvent; leaving the mixture at room temperature to prepare an Aloe vera root extract; and preparing a cosmetic composition comprising the Aloe vera root extract.

The Aloe vera irregular root may be characterized in that it is produced by in-flight culture. Aloe vera root produced by the above in-flight culture method is fundamentally free from harmful ingredients such as residual pesticides (eg, Diazinon, DDT, Metalaxyl) and heavy metals (eg, lead, cadmium) and virus infection.

The Aloe vera root and the organic solvent may be mixed in a weight ratio of 1:8 to 1:12. For example, the Aloe vera root and organic solvent may be mixed in a weight ratio of 1:8 to 1:10, 1:8 to 1:9, 1:9 to 1:12, or 1:10 to 1:12. have. In this case, the Aloe vera root may be prepared by drying the Aloe vera root extract.

The organic solvent may be characterized in that it comprises at least one selected from the group consisting of methanol, ethanol, propanol, butanol, and combinations thereof. Specifically, the organic solvent may be ethanol.

The Aloe vera root extract may be characterized in that it is prepared by leaving the mixture at room temperature for 22 to 26 hours.

The cosmetic composition for improving skin according to the present invention may further include, in the step of preparing the Aloe vera root extract, the mixture is left at room temperature, filtered using a filter, and then concentrated under reduced pressure and dried with hot air.

The Aloe vera root extract prepared according to the present invention has a higher nitric oxide (NO) production inhibitory ability and free radicals compared to the Aloe vera root extract and Aloe vera (leaf) organic solvent or water extract extracted using water rather than an organic solvent. It can exhibit the ability to remove. In addition, the Aloe vera root extract prepared according to the present invention differs from the Aloe vera root extract and Aloe vera (leaf) organic solvent or water extract extracted using water, not an organic solvent, a matrix metalloprotein that causes wrinkles. It may be effective in preventing and improving skin wrinkles by effectively inhibiting the production of degrading enzyme (MMP).

Hereinafter, the present invention will be described in more detail through Examples and the like according to the present invention, but the scope of the present invention is not limited by the Examples presented below.

<Preparation Example: Preparation of Aloe vera root extract>

(1) Aloe vera root culture

The culture of Aloe vera irregular root was prepared according to the present inventor's invention, Korean Patent Registration No. 10-1576787.

Specifically, the leaves of young aloe vera seedlings were washed cleanly on the flowing leaves, and the tissue was cut, leaving a pale yellow part, and then immersed in 4% lacquer to sterilize the surface without agitation for 5 minutes. After the surface sterilization was completed, the surface was washed several times with sterile water. The washed tissue was cultured in MS medium containing 0.5 mg/L NAA solidified with 0.7 g/L plate agar at 28° C. in the dark state for 3 weeks. The induced irregular root was transferred to a liquid medium containing 0.3 mg/L IBA in MS medium and cultured.

1 is a photograph showing Aloe vera irregular root induced and proliferated by tissue culture technique: FIG. 1 (A) is a fixed root induced in MS solid medium with 0.5 mg/L NAA added after 4-5 weeks, FIG. 1 (B) of (B) is an irregular root proliferated in MS broth supplemented with 0.5 mg/L NAA and 0.2 mg/L BA after 8-10 weeks.

(2) Aloe vera root 70% ethanol extract preparation

100 ml of 70% (v/v) ethanol aqueous solution was added to 10 g of lyophilized dried Aloe vera root, and extracted at room temperature for 24 hours, filtered through a 0.45 μm filter, and concentrated under reduced pressure and dried with hot air. .

(3) Aloe vera root water extract preparation

After adding 100 ml of purified water to 10 g of dried aloe vera root, extraction was performed at room temperature for 24 hours, filtered through a 0.45 μm filter, and concentrated under reduced pressure and dried with hot air.

(4) Aloe vera 70% ethanol extract preparation

After adding 100 ml of 70% (v/v) aqueous ethanol solution to 10 g of dried Aloe vera leaves, extraction was performed at room temperature for 24 hours, filtered through a 0.45 μm filter, concentrated under reduced pressure, and dried with hot air.

(5) Aloe vera water extract preparation

After adding 100 ml of purified water to 10 g of dried aloe vera leaves, extraction was performed at room temperature for 24 hours, filtered through a 0.45 μm filter, concentrated under reduced pressure, and dried with hot air.

<Example 1: Measurement of cytotoxicity>

Cytotoxicity was measured for water or ethanol extracts of Aloe vera root and Aloe vera (leaf) prepared in Preparation Example.

Mouse macrophages (RAW264.7) induced with an inflammatory response with 0.5 μg/ml LPS (lipopolysaccharide) were seeded at 2×105 cells/well in a 96-well plate and cultured for 24 hours, followed by NMMA 50 μg/ ㎖, indomethacin (Indomethacin) 2 ng / ㎖ and each extract sample was treated with 100 or 200 ㎍ / ㎖ and incubated for 48 hours, the cell viability was measured by performing the MTT assay (assay).

As a result, as shown in FIG. 2 , it was found that the Aloe vera or Aloe vera irregular root extract did not show cytotoxicity.

<Example 2: Anti-inflammatory activity assay>

In the same manner as in Example 1, RAW264.7 cells induced by LPS were aliquoted into a 96-well plate and cultured for 24 hours, treated with Aloe vera or Aloe vera root extract samples, respectively, and cultured for 48 hours. Then, the amount of nitric oxide (NO) produced was measured using a Griess reagent.

As a result, as shown in FIG. 3, NO production was not inhibited in the NMMA (Mono-methyl-L-arginine) treatment group, but in the indomethacin treatment group (indo), it was confirmed that the NO production increased by the LPS treatment was reduced. could In addition, it was confirmed that NO production was suppressed in the group treated with 70% ethanol extract of Aloe vera root. It was found that both the water extract of Aloe vera root, the water extract of Aloe vera, and the ethanol extract using the same sample had no NO production inhibitory activity.

<Example 3: Antioxidant activity analysis>

The antioxidant activity of 70% ethanol extract of Aloe vera or Aloe vera root root prepared in Preparation Example was analyzed.

Briefly, 190 μl of 0.1 mM DPPH (2,2-diphenyl-1-picrylhydrazyl) and 10 μl of the extract were mixed, reacted in the dark for 30 minutes, and absorbance was measured at 517 nm to evaluate radical scavenging ability.

As a result, as shown in Table 5 below (comparison of DPPH free radical scavenging activity), it was confirmed that the 70% ethanol extract of Aloe vera root had a stronger DPPH radical scavenging activity than the 70% ethanol extract of Aloe vera.

division aloe vera root
ethanol extract aloe vera
ethanol extract IC50 (μg/ml) 15.2 ± 3.7 162.5 ± 4.7

<Example 4: Wrinkle improvement efficacy evaluation>

In order to confirm the anti-wrinkle effect of the extract prepared in Preparation Example, the effect of inhibiting the synthesis of matrix metalloprotease-1 (MMP-1) and MMP-3 protein and the facilitation of procollagen protein synthesis was analyzed.

(1) Analysis of the effect of inhibiting the synthesis of MMP-1 and MMP-3 proteins

2 ㎖ of DMEM culture solution was put in a 40 mm cell culture dish, and human fibroblast cells were inoculated at a concentration of about 1.2×10 5 cells/dish, and then cultured at 37° C. and 5% carbon dioxide environment for 24 hours. Then, after irradiating UVB under the condition of 144 mJ/cm ² , the medium containing each extract sample was replaced and cultured for 3 days. Thereafter, the cell culture medium was recovered and centrifuged at 4° C., 7,500 rpm for 5 minutes to recover the supernatant, and MMP-1 using a Human total MMP-1/3 kit (R&D Systems, Inc., Minneapolis, MN, USA). and MMP-3 protein expression levels were confirmed.

As a result, the amount of MMP-1 protein production in the human fibroblasts treated with UV irradiation increased about 2 times compared to the UV-untreated group. Alternatively, it was confirmed that the levels were reduced by 65.1% and 21.2%, respectively, after treatment with the Aloe vera ethanol extract at 200 μg/ml concentration. . Through this, it was confirmed that the ethanol extract of Aloe vera irregular root very effectively inhibited the production of MMP-1 (FIG. 4(A)).

In terms of the amount of MMP-3 protein production, it was observed that the ethanol extract of Aloe vera root or the ethanol extract of Aloe vera had an inhibitory effect of 37.2% and 21.4%, respectively, of 37.2% and 21.4%, respectively, under 200 μg/ml treatment conditions (Fig. 4 (B)). In particular, although the same Aloe vera root was used, it was observed that such an effect of inhibiting the production of MMP protein was not confirmed when extracted with water.

Through these results, it can be confirmed that the ethanol extract of Aloe vera root of the present invention can be effectively used to prevent and improve skin wrinkles by effectively inhibiting the production of MMP-1 and MMP-3 proteins, which are the cause of wrinkles. there was.

(2) Analysis of the effect of promoting the synthesis of procollagen protein

In order to confirm the effect of each extract of the present invention on collagen production, cells were prepared in the same manner as the experimental conditions for confirming the synthesis inhibitory effect of the MMP protein, and the protein expression level of type I procollagen in the culture medium of UVB-irradiated cells Changes were confirmed using the Procollagen Type IC Peptide EIA kit (Takara, Shiga, Japan).

As a result, the UV-treated human fibroblasts significantly reduced the production of type I procollagen protein compared to the UV-untreated group, and the reduced production of type I procollagen protein was the ethanol extract of Aloe vera root or Aloe vera. It was confirmed that the ethanol extract was increased by 379.4% and 261.5%, respectively, under the condition of 200 μg/ml ( FIG. 5 ). As in the MMP protein production inhibition test, the water extract of Aloe vera root was not confirmed to have a procollagen production enhancing effect.

Based on the results of (1) analysis of the effect of MMP-1 and MMP-3 protein synthesis inhibition and (2) analysis of the procollagen protein synthesis promoting effect, the 70% ethanol extract of Aloe vera root of the present invention was prepared as Type I Pro It was confirmed that it has the effect of promoting the production of collagen and inhibiting the expression of collagen-degrading enzymes MMP-1 and MMP-3, thereby effectively preventing the generation of wrinkles and improving the generated wrinkles.

<Example 5: Evaluation of Whitening Efficacy>

In order to evaluate the melanin synthesis inhibitory effect of the extract prepared in Preparation Example, mouse melanoma cells (B-16 melanoma cells) were cultured using DMEM medium containing 10% FBS, and 3 × 105 in a 6-well plate. After dividing into cells/well and culturing for 24 hours, each extract at a concentration of 50 μg/ml kojic acid or 50, 100 and 200 μg/ml was treated, and cultured for 3 days. After incubation, the cells were washed twice with PBS and trypsinized to remove the cells from the culture vessel and centrifuged to obtain a cell precipitate. 1 ㎖ of 1N sodium hydroxide solution was added to the extracted melanin, boiled for about 10 minutes to dissolve the melanin, and the absorbance was measured at 400 nm. The amount of melanin was obtained by a standard straight line prepared using synthetic melanin, and the amount of melanin in the experimental group was calculated as a percentage of the amount of melanin in the control group. In addition, the toxicity of the extract to B-16 cells was analyzed through MTT assay.

As a result, neither water nor 70% ethanol extract of Aloe vera or Aloe vera root root showed cytotoxicity (FIG. 6), and it was confirmed that Aloe vera and water extract of Aloe vera root root had no ability to inhibit melanin synthesis. However, 70% ethanol extract of Aloe vera root showed melanin synthesis inhibitory effects of 83.8, 75.7 and 59.5%, respectively, at 200, 100 and 50 μg/ml concentration treatment conditions, and Aloe vera 70% ethanol extract showed 200, 100 and 50 μg/ml concentration, respectively. It was confirmed that 29.1, 22.5, and 0% melanin synthesis inhibitory effects were shown at the 50 μg/ml concentration treatment condition, respectively, so that the ethanol extract of Aloe vera root had significantly superior melanin synthesis inhibitory effect compared to the Aloe vera ethanol extract (Fig. 7). ).

In addition, in order to confirm the activity of the tyrosinase enzyme required for melanin synthesis, the tyrosinase activity inhibitory ability of the ethanol extract of Aloe vera root and the ethanol extract of Aloe vera was analyzed.

Briefly, after mixing 220 μl of 0.1 M sodium phosphate buffer and 20 μl of an extract sample of 200 μg/ml, 40 μl of 1.5 mM L-tyrosine and 20 μl of mushroom tyrosinase prepared at 1,500 units/ml were added After reacting at 37° C. for 15 minutes, absorbance was measured at 490 nm to evaluate the amount of generated dopachrome.

As a result, as shown in Table 6 (Comparison of tyrosinase inhibitory activity), it was confirmed that the tyrosinase activity inhibitory ability of the 70% ethanol extract of Aloe vera root 70% did not show a significant difference from that of the Aloe vera 70% ethanol extract.

division Aloe Vera Root Ethanol Extract Aloe Vera Ethanol Extract Tyrosinase activity inhibition ability (%) 28.5 ± 4.8 30.2 ± 5.5

From the above results, it was possible to infer that the 70% ethanol extract of Aloe vera root root had an excellent effect of inhibiting the synthesis of melanin pigment, but that it was an effect due to a mechanism other than the effect by inhibiting the activity of the tyrosinase enzyme.

Claims (9)

Containing Aloe vera root extract as an active ingredient of a cosmetic composition having skin wrinkle improvement and whitening effects,
A cosmetic composition for improving skin comprising the Aloe vera root extract in an amount of 1 to 5% by weight based on the total weight of the cosmetic composition.
According to claim 1,
The aloe vera root extract is a cosmetic composition for improving skin, characterized in that it has anti-inflammatory and antioxidant activity.
According to claim 1,
The aloe vera root extract promotes the production of procollagen and has an activity of inhibiting melanin and matrix metalloprotease (MMP), a cosmetic composition for improving skin.
According to claim 1,
The composition includes skin, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nourishing lotion, massage cream, nourishing cream, eye cream, moisture cream, hand cream, essence, nourishing essence, pack, cleansing foam, A cosmetic composition, characterized in that at least one formulation selected from the group consisting of cleansing water, cleansing lotion, cleansing cream, body lotion, body cleanser, soap and powder.
preparing a mixture by supporting Aloe vera root in an organic solvent;
leaving the mixture at room temperature to prepare an Aloe vera root extract; and
A method for manufacturing a cosmetic composition for skin improvement, comprising preparing a cosmetic composition comprising the Aloe vera root extract.
6. The method of claim 5,
The method for producing a cosmetic composition for improving skin, characterized in that the Aloe vera irregular root is produced by in-flight culture.
6. The method of claim 5,
The method for producing a cosmetic composition for skin improvement, characterized in that the Aloe vera root and the organic solvent are mixed in a weight ratio of 1:8 to 1:12.
6. The method of claim 5,
The organic solvent is a method for producing a cosmetic composition for skin improvement, characterized in that it comprises at least one selected from the group consisting of methanol, ethanol, propanol, butanol, and combinations thereof.
6. The method of claim 5,
The method for producing a cosmetic composition for improving skin, wherein the Aloe vera root extract is prepared by leaving the mixture at room temperature for 22 to 26 hours.

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