KR20210117859A - Method of manufacture of plant fungicide and plant fungicide thereof - Google Patents

Abstract

The present invention relates to a method for manufacturing a fungicide for plant diseases by combining sulfur, quicklime, biotite, and a stevia fermented strain acquired from a stevia plant. According to the present invention, the method comprises: a mixture preparation step of mixing any one selected from a group consisting of quicklime, biotite, muscovite, phosphinic mica, sericite, paragonite, parasite, illite, margarite, and crintonite in a mixture of water and sulfur; a mixture heating step of adding water to the mixture and heating the mixture; a mixture separation step of storing the mixture in a precipitation tank for a predetermined time and acquiring a pure liquid mixture from which microcrystals mixed in the mixture are separated; a raw fungicide solution acquisition step of purifying the mixture with a filter to acquire a raw fungicide solution; and a commercialization step of administering a stevia fermented strain acquired from a stevia plant to the raw fungicide solution and aging the mixture for a predetermined time to complete and commercialize a fungicide. Accordingly, the fungicide for plant diseases provides an excellent effect on browning resistance against phytopathogenic fungi, especially anthrax, rare powdery mildew, grayish mold, microflora, and filamentous fungi, and blocks penetration of fungi or inhibiting proliferation even by spraying a predetermined amount on plants to promote growth of the plants.

Description

Method of manufacturing a fungicide for plant diseases and a fungicide manufactured by the method {Method of manufacture of plant fungicide and plant fungicide thereof}

The present invention relates to a fungicide for plant diseases that can promote plant growth by blocking or inhibiting the proliferation of pests and diseases occurring in plants.

Among various crops, especially phytopathogenic fungi that occur in horticultural crops directly or indirectly harm horticultural crops, so in order to harvest a lot of high-quality horticultural crops, spray chemical pesticides or partially spray growth promoting or growth inhibitors. are doing

Phytopathogenic fungi are the main causes of reduction in yield and quality by bringing diseases to crops such as various vegetables, fruits, and flowers. cladosporioides), pepper anthracnose (Colletotrichum gloeosporioides), ginseng root blight (Cylindrocarpon destructans), melon wilt blight (Didymella bryoniai), tomato wilting blight (Fusarium oxysporum), tomato leaf fungus (Gibberella fulvum), tomato leaf fungus (Fulvia) fujikuroi), pepper blight (Phytophthora capsici), rice blight blight (Rhizoctonia solani), sweet potato soft blight (Rhizopus nigricans), onion sclerotium sclerotium sclerotium (Sclerotium cepivorum), tomato spot blight (Stemphylium lycopersici), etc. there is

Among them, late blight has strong pathogenicity to host plants and is a primary invader that breaks down and invades the cell wall of the host even without a wound, and most belong to the genus Phytophthora. Since the late blight does not invade the diseased tissue secondarily, the blight isolated from the diseased plant is the main cause of most of the disease.

In particular, pepper blight greatly reduces the production of pepper during pepper cultivation, and pepper blight that causes pepper blight is a semi-aquatic bacteria that evolved from water and settled on land, multiplying in water and propagating along the water. Since red pepper blight is almost impossible to treat once it occurs, it is best to prevent the disease from occurring, and it is known that it is important to prevent the growth of pathogens and prevent the occurrence of diseases by applying registered drugs prophylactically. Also, if the disease has already occurred, it is important to prevent further spread.

Methods such as cultivation and rotation of resistant varieties are also performed to control late blight, but the most effective control method known so far is a chemical control method using an organically synthesized fungicide. The amount of spraying of pesticides due to such chemical control is increasing every year, and due to the excessive use of chemical pesticides, plant pathogens acquire resistance to chemical pesticides, residual toxicity of chemical pesticides, soil acidification, toxicity to livestock, and effects on natural enemies , pollution of drinking water sources, destruction of ecosystems, etc., are becoming more and more serious, and the resulting social problems are also getting serious.

Korean Patent Application No. 10-2012-0080467

The present invention sprays chemical pesticides to remove and block pests such as phytopathogenic fungi occurring in various plants, and acquires resistance to chemical pesticides of plant pathogens due to overused chemical pesticides, residual toxicity of chemical pesticides , to solve problems such as acidification of soil, toxicity to livestock, effects on natural enemies, contamination of drinking water sources, and destruction of ecosystems. The main purpose of the present invention is to provide a fungicide for plant diseases that control diseases and pests.

However, the problems to be solved by the present invention are not limited to the problems mentioned above, and other problems not mentioned will be clearly understood by those skilled in the art from the following description.

Therefore, the present invention for solving the above problems, in the method for producing a fungicide for plant diseases, in a mixture of water and sulfur, quicklime, biotite, muscovite, phlogopite, sericite, paragonite, seaweed, illite, a mixture generating step of mixing any one selected from the group consisting of margarite and crintonite;

adding water to the mixture and heating the mixture;

a mixture separation step of storing the mixture in a precipitation tank for a predetermined time, and obtaining a pure liquid mixture in which the microcrystals mixed in the mixture are separated;

Purifying the mixture with a filter to obtain a disinfectant stock solution to produce a disinfectant stock solution; and

Administering a stevia fermented strain obtained from a stevia plant to the sterilant stock solution, aging for a predetermined time to complete the sterilizing agent, and commercializing;

The stevia fermentation strain is,

semi-drying the stevia plant;

After placing the stevia plant in a blackout bag, inoculating any one of Camembertti (Penicillium camemberti), Penicillium roqueforti or Penicillium glaucum;

culturing and propagating the strain by storing the stevia plant at a predetermined temperature; and

Forming a stevia extract by putting the stevia plant in water and heating it; characterized in that it is formed including.

The present invention is a fungicide for plant diseases prepared by blending a stevia fermented strain obtained from a sulfur family and a stevia plant. It has an excellent effect, and even by spraying a predetermined amount on the plant, there is a remarkable effect of blocking the penetration of fungi or inhibiting the growth of the plant to promote the growth of the plant.

In describing a preferred embodiment of the present invention in detail, if it is determined that a detailed description of a related well-known configuration or function may obscure the gist of the present invention, the detailed description will be omitted.

The fungicide for plant parallel use of the present invention is a mixture of water and sulfur, with quicklime, biotite, muscovite, phlogopite, sericite, paragonite, parasite, illite, margarite, crintonite, any one selected from the group is mixed to form a sterilant stock solution, and is formed by further mixing the stevia fermentation strain with the sterilant stock solution.

In addition, the method for producing the fungicide for plant diseases of the present invention is selected from the group consisting of quicklime, biotite, muscovite, phlogopite, sericite, paragonite, parasite, illite, margarite, and crintonite in a mixture of water and sulfur. A mixture generating step (S100) of mixing any one to produce a liquid mixture; Adding more water to the mixture, heating the mixture to a predetermined temperature to brew the mixture (S200); A mixture separation step of storing the mixture in a precipitation tank for a predetermined time, precipitating the microcrystals and foreign substances mixed in the mixture into the lower part of the settling tank, and separating a liquid mixture of pure water from which the microcrystals and foreign substances are separated in the settling tank (S300); Purifying the mixture separated in the settling tank with a filter to obtain a sterilant stock solution to produce a sterilant stock solution (S400); and administering a stevia fermented strain obtained from a stevia plant to the sterilant stock solution, aging for a predetermined time to complete the sterilizing agent, and commercializing (S500); characterized in that it comprises a.

The present invention can inhibit or prevent diseases caused by fungi such as anthrax, powdery mildew, red mold, eczema mold, gray mold, dandruff disease, filamentous fungi, etc. It relates to a fungicide for plant diseases that can be safely and reliably grown from

A method for producing a disinfectant for plant diseases and a method for producing a disinfectant for plant diseases according to the present invention for achieving the above object will be described in detail through examples.

The fungicide for plant diseases of the present invention is first mixed with water, sulfur, quicklime and biotite in a predetermined ratio.

Preferably, 15 kg of sulfur, 7 to 10 kg of quicklime and 1 to 2 kg of biotite are prepared with respect to 60 L of water, and these are mixed.

The sulfur is preferably prepared in a powder form without lumps, but is not limited to either powder or liquid.

The biotite (黑雲母, biotite) is, muscovite, phlogopite, sericite, paragonite, sea rock, glauconite , illite (illite), margarite (margarite), may be replaced with any one selected from the clintonite (clintonite) group.

Add sulfur and water to a mixing tank of a predetermined size, boil over high heat, and stir to dissolve the sulfur in the water.

At a time when sulfur boils and air bubbles rise in the mixing tank, quicklime and biotite are added little by little to the mixing tank. At this time, a chemical reaction between sulfur and quicklime and/or biotite may occur intensely in the mixing tank, which may cause the contents inside the mixing tank to overflow from the mixing tank. Put it in the jaw and stir it.

According to the present invention, quicklime and biotite may be further mixed sequentially in a mixing tank containing water and sulfur to produce a liquid mixture, or the quicklime and biotite may be mixed, and these mixtures may be added to a mixing tank containing sulfur and water.

Since sulfur has a melting point of 112.8° C. and cannot be completely dissolved in water to dissolve sulfur, the sulfur can be completely melted by adding the quicklime and generating high-temperature heat through a chemical reaction.

In addition, by mixing in a ratio of 15 kg of sulfur and 7 to 10 kg of quicklime with respect to 60 L of water, sulfur is safely dissolved in water in the fastest time. When the sulfur is mixed with more or less than the set amount, there is a problem that it is not completely soluble in water or overflows frequently in the mixing tank due to high heat.

The biotite is to provide a large amount of minerals while melting due to high heat, and may be replaced with any one selected from the group consisting of muscovite, phosphite, sericite, paragonite, parasite, illite, margarite and clintonite. .

When an intense chemical reaction occurs in the mixing tank, it is preferable to prevent the mixture in the mixing tank from boiling over by controlling the amount of quicklime added or by controlling the heating temperature.

In the mixing tank, water, sulfur, quicklime and biotite are mixed to form a liquid mixture, and the mixture is boiled until about 60 L remains in the mixing tank (mixture generation step (S100)).

Next, as water, sulfur, quicklime and biotite are mixed in the mixing tank, a chemical reaction occurs, a mixture is formed, and then, when the chemical reaction stops, the mixture is transferred to the calcination tank. Then, a calcination step of heating to a predetermined temperature by adding more water to the calcination tank containing the mixture is performed (mixture calcination step (S200)).

When the chemical reaction of the mixture is finished, about 20 to 30 L of water is further added to the calcination tank, and heated over high heat for a predetermined time. At this time, boil the mixture in the calcination tank until about 60L remains. Then, with about 60L of the mixture remaining in the calcination tank, 20 ~ 30L of water is additionally filled, and the mixture is heated again so that only about 70L of the mixture inside the calcining tank remains.

And the mixture obtained through the calcination step (S200) is transferred to a clean settling tank and stored for a predetermined time (about 1 to 2 days), and fine crystals or foreign substances mixed in the mixture are precipitated in the lower part of the settling tank. By separating the fine crystals or foreign substances and the pure liquid mixture from which the precipitate is removed into upper and lower layers, only the upper pure liquid mixture can be easily separated in the settling tank (mixture separation step (S300)).

And the liquid mixture separated in the settling tank is moved to the refining tank and purified through a filter provided in the refining tank to obtain a sterilant stock solution (sterilant stock solution obtaining step (S400)).

The filter used in the present invention includes a charcoal filter, a biotite filter, a serpentine filter, a quartz filter, and a cell culture filter in which elvan and mud are mixed.

The pure mixture separated from the settling tank and transferred to the refining tank is first passed through a charcoal filter, and then passed through a biotite filter, a serpentine filter, a quartz filter, and a cell culture filter in which elvan stone and mud are mixed. will be refined by

The order in which the mixture passes through the filter is not limited to the above order and may be changed.

The filter forms a ball with a smooth flow of water in a heating furnace at 1,200° C., and uses it by firing it.

The cell culture filter in which the elvan stone and the mud are mixed is formed by mixing the elvan stone and the mud in a ratio of 5:5, and then the filter manufacturing method is used, and the work of applying the prepared strain is carried out. It is preferable to use the cultured strain after soaking it in water for about 1 week.

On the other hand, the sterilant stock solution obtained through the filter in the purification tank is transferred to a maturation tank set at 40 ~ 45 ° C. Here, a stevia fermentation strain obtained using a stevia plant is administered to the disinfectant stock solution, and it is aged for about 3 to 5 days. By doing so, the plant fungicide according to the present invention is completed and commercialized. (Production step (S500)).

The temperature of the aging tank is most preferably a temperature of 40 ~ 45 ℃, at a temperature below 40 ℃, the fermentation and ripening of the strain is slow due to a delay, and at a temperature of 45 ℃ or more, most of the strains die due to the high temperature. There is this.

The Stevia (Stevia) plant is a perennial rooted plant of the Asteraceae family, and grows around a river or wetland. In the root, the development of the original root is not clear and there are many side roots and membrane roots. In the later stages of growth, thick roots develop, resulting in a storage function. A new branch grows from the hidden eye close to the root, forming a new stem every year. Stems stand upright and the stem loses its function during wintering. Leaves hang on the stem nodes and branches sprout from the leaf axils. The leaves are opposite, lanceolate, 4-10cm long and about 2.5cm wide. It has fine sawtooths and curves, and there are 3 leaf veins. There is no petiole, and there are fine hairs all over the stem. A flower blooms in clusters of 5-6 ornamental flowers per cluster, and has the property of self-incompatibility. As it is a single plant, it blooms quickly under single conditions. The seeds have a low fruiting rate and are very small. At the front end of the seed, there is a scallop-shaped tube hair. It is native to the mountainous regions of South America, such as Paraguay, Argentina, and Brazil. In Paraguay, stevia leaves have been used as a sweetener since ancient times. Leaves contain stevioside, a sweetener, about 6-7% by weight, and the content varies greatly depending on the individual. The sweetening ingredient is 300 times that of sugar, and it is used as a sweetener for soft drinks, such as making tea or as a substitute for chewing gum. It was introduced to Korea in 1973 and has been cultivating new varieties. Propagation is by seeds or cuttings. Harvesting can be done 1-2 times a year, and early and mid-September is the best time. Stevia contains potassium, sodium, beta-carotene, vitamin A, vitamin B2, vitamin B6, vitamin E, inorganic salts and nutrients necessary for the human body, and has strong antioxidant, sterilizing, and detoxifying effects. In particular, it is known that it can be used for the purpose of restoring soil or plants contaminated with dioxin because it has a dioxin decomposition ability.

Stevia fermentation strain using a stevia plant having the above characteristics is cultured in the following way.

First, the stevia plant is harvested, and it is semi-dried (D100).

The stevia plant is most preferably grown to a size of about 30-50 cm.

Place the semi-dried stevia plants in a black plastic bag or blackout bag, and mix any one or at least two of Penicillium camemberti, Penicillium roqueforti and Penicillium glaucum Inoculate one stevia plant (D200).

The penicillin camemberti (Penicillium camemberti), penicillium roqueforti (Penicillium roqueforti) and penicillin glow foam (Penicillium glaucum) is a strain that produces polysaccharides, stevioside, It has a remarkable effect, helping to become a prey active polysaccharide component.

In addition, the penicillin camemberti and penicillin glow foam have a remarkable effect of inducing them to have an allergen and blocking severe mold allergy.

Stevia plants that have undergone the "D200" step are stored at a temperature of 35 to 40° C. for 7 to 8 days, and the strain is allowed to propagate in Stevia (D300).

And the strain cultured stevia and water are mixed in a ratio of 1:2, for example, about 5 kg of strain is put in cultured stevia and 10L of water, and this is heated for about 3 to 4 days. Therefore, the components of stevia and the cells cultured in stevia seep out in the water, thereby obtaining stevia extract (D400).

And finally, the obtained Stevia extract is an archaea strain (Archaea), Deinococcus radiodurans (Deinococcus radiodurans) strain or Sulfolobus solataricus (Sulfolobus solfataricus) strain is blended (D500).

The Deinococcus radiodurans strain is a representative strain belonging to the genus Deinococcus, and when administered to a plant in combination with the Stevia strain, the cell membrane extract of the Deinococcus radiodurans strain is the ultraviolet light of the plant. It improves the protective ability against UV rays and the ability to recover from UV rays, and has excellent effects such as lipid and oxidation prevention, whitening effect, especially anti-allergy.

When the Deinococcus radiodurans strain is not mixed with the Stevia strain and only the Stevia strain is administered to the plant, the antioxidant action, sterilization action, and detoxification action by the Stevia plant can be obtained, but the ability to cope with UV protection of the plant This is remarkably reduced, and plants exposed to UV light have discoloration or curling of leaves due to a decrease in recovery ability, and gradually wither and die.

In addition, the Sulfolobus solfataricus, a sulfur (S)-dependent hyperthermophilic microorganism with an optimum temperature of 89 ° C., is one of the best-studied archae bacteria, and a large amount of biomass can be easily obtained, and when administered to plants in combination with the stevia strain, it grows at a very high temperature and thus provides a source of enzymes having abnormal physicochemical properties.

The present invention is to obtain a stevia fermented strain with excellent anti-allergic efficacy by blending the archaea strain with stevia extract obtained from a stevia plant, and administering about 3 to 5% of the stevia fermented strain to the prepared fungicide stock solution, By aging it for about 3 to 5 days, the fungicide for plant diseases according to the present invention is completed.

And the fungicide of the present invention is regularly sprayed on plants in units of 1 week or 10 days.

Therefore, the present invention is a fungicide for plant diseases prepared by blending the stevia fermented strain obtained from the Sulfuraceae family and Stevia plant, and has a browning resistance to phytopathogenic fungi, particularly anthrax, rare powdery mildew, grayish mold, microflora, filamentous fungi, etc. There is this excellent effect, and even by spraying a predetermined amount on the plant, there is a remarkable effect of blocking the penetration of fungi or inhibiting the growth of the plant to promote the growth of the plant.

The configuration shown in the embodiments and drawings described in the present specification as described above is only the most preferred embodiment of the present invention and does not represent all of the technical spirit of the present invention, so various equivalents and modifications that can be substituted for them It should be understood that there may be examples.

Claims (5)

In the method for producing a fungicide for plant diseases,
Mixture generation step of mixing any one selected from the group consisting of biotite, muscovite, phosphinic mica, sericite, paragonite, parasite, illite, margarite, and crintonite in a mixture of water, sulfur and quicklime;
adding water to the mixture and heating the mixture;
a mixture separation step of storing the mixture in a precipitation tank for a predetermined time, and obtaining a pure liquid mixture in which the microcrystals mixed in the mixture are separated;
Purifying the mixture with a filter to obtain a disinfectant stock solution to produce a disinfectant stock solution; and
Administering a stevia fermented strain obtained from a stevia plant to the sterilant stock solution, aging for a predetermined time to complete the sterilizing agent, and commercializing;
The stevia fermentation strain is,
semi-drying the stevia plant;
After placing the stevia plant in a blackout bag, inoculating any one of Camembertti (Penicillium camemberti), Penicillium roqueforti or Penicillium glaucum;
culturing and propagating the strain by storing the stevia plant at a predetermined temperature; and
A method of producing a fungicide for plant diseases, characterized in that it is formed including; putting the stevia plant in water and heating to form a stevia extract.
According to claim 1,
The stevia fermentation strain further comprises; after forming the stevia extract, blending the archaea strain with the stevia extract;
The archaea strain,
Deinococcus radiodurans (Deinococcus radiodurans) strain or Sulfolobus solatanicus (Sulfolobus solfataricus) Method for producing a fungicide for plant diseases, characterized in that it comprises a strain.
According to claim 1,
The step of forming the stevia extract is,
A method for producing a fungicide for plant diseases, characterized in that the strain is formed by mixing the cultured stevia plant and water in a ratio of 1:2, and heating for 3 to 4 days.
According to claim 1,
In the stage of completing the disinfectant,
A method for producing a disinfectant for plant diseases, characterized in that it is completed by administering a stevia fermented strain to the sterilant stock solution in a maturation tank, and aging at a temperature of 40 to 45° C. for 3 to 5 days.
A fungicide for plant diseases produced by the method according to any one of claims 1 to 4.