KR20210091250A - Car-t 세포를 생성하기 위한 이중유전자 벡터 및 이의 용도 - Google Patents

Car-t 세포를 생성하기 위한 이중유전자 벡터 및 이의 용도 Download PDF

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KR20210091250A
KR20210091250A KR1020217017844A KR20217017844A KR20210091250A KR 20210091250 A KR20210091250 A KR 20210091250A KR 1020217017844 A KR1020217017844 A KR 1020217017844A KR 20217017844 A KR20217017844 A KR 20217017844A KR 20210091250 A KR20210091250 A KR 20210091250A
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seq
cells
car
promoter
sequence identity
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리잘 이즈마일
윈친 이
머레이 로빈슨
잉 심 탄
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메디식스 테라퓨틱스 피티이 엘티디.
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KR1020217017844A 2018-11-14 2019-11-14 Car-t 세포를 생성하기 위한 이중유전자 벡터 및 이의 용도 Ceased KR20210091250A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862767069P 2018-11-14 2018-11-14
US62/767,069 2018-11-14
PCT/US2019/061549 WO2020102589A1 (en) 2018-11-14 2019-11-14 Two-gene vectors for generating car-t cells and uses thereof

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KR20210091250A true KR20210091250A (ko) 2021-07-21

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KR1020217017844A Ceased KR20210091250A (ko) 2018-11-14 2019-11-14 Car-t 세포를 생성하기 위한 이중유전자 벡터 및 이의 용도

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US (1) US20210395779A1 (https=)
EP (1) EP3864052A4 (https=)
JP (2) JP2022512994A (https=)
KR (1) KR20210091250A (https=)
CN (2) CN113227144B (https=)
AU (1) AU2019378039A1 (https=)
CA (1) CA3119296A1 (https=)
MA (1) MA53877A (https=)
SG (1) SG11202104334SA (https=)
WO (1) WO2020102589A1 (https=)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113713091A (zh) 2015-02-06 2021-11-30 新加坡国立大学 工程免疫细胞及其用途和生产方法
SG11201903830TA (en) 2016-11-22 2019-05-30 Nat Univ Singapore Blockade of cd7 expression and chimeric antigen receptors for immunotherapy of t-cell malignancies
GB201719646D0 (en) 2017-11-27 2018-01-10 Bivictrix Therapeutics Ltd Therapy
CN112300282A (zh) * 2020-11-03 2021-02-02 南京北恒生物科技有限公司 靶向cd7的人源化抗体及其用途
CN114525259B (zh) * 2020-11-03 2025-02-14 南京北恒生物科技有限公司 靶向cd7的嵌合抗原受体及其用途
CN114525306B (zh) * 2020-11-23 2025-01-28 博生吉医药科技(苏州)有限公司 一种cd7-car-t细胞的制备方法及应用
GB202020572D0 (en) * 2020-12-23 2021-02-03 Bivictrix Therapeutics Ltd Novel methods of therapy
GB202020573D0 (en) * 2020-12-23 2021-02-03 Bivictrix Therapeutics Ltd Novel methods of therapy
CN115786271B (zh) * 2021-01-12 2025-07-22 上海雅科生物科技有限公司 靶向cd7的工程化免疫细胞、嵌合抗原受体、cd7阻断分子及应用
US20250099504A1 (en) * 2022-03-29 2025-03-27 Institute Of Hematology And Blood Diseases Hospital , Chinese Academy Of Medical Sciences Antibody that specifically binds to cd7 and use thereof in preparing chimeric antigen receptor
CN120025438A (zh) * 2023-11-21 2025-05-23 合源生物科技(天津)有限公司 一种靶向cd7的人源化抗体、嵌合抗原受体及其应用
CN118221810A (zh) * 2024-03-05 2024-06-21 武汉生物制品研究所有限责任公司 一种能够结合ev-a71的单克隆抗体及其应用
CN120865430A (zh) * 2024-04-30 2025-10-31 合源生物科技(天津)有限公司 一种增强型嵌合抗原受体及其应用

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5260203A (en) 1986-09-02 1993-11-09 Enzon, Inc. Single polypeptide chain binding molecules
US4946778A (en) 1987-09-21 1990-08-07 Genex Corporation Single polypeptide chain binding molecules
ATE87659T1 (de) 1986-09-02 1993-04-15 Enzon Lab Inc Bindungsmolekuele mit einzelpolypeptidkette.
CA2386270A1 (en) 1999-10-15 2001-04-26 University Of Massachusetts Rna interference pathway genes as tools for targeted genetic interference
US6326193B1 (en) 1999-11-05 2001-12-04 Cambria Biosciences, Llc Insect control agent
AU2001275474A1 (en) 2000-06-12 2001-12-24 Akkadix Corporation Materials and methods for the control of nematodes
DK2958943T3 (da) * 2013-02-20 2019-12-09 Univ Pennsylvania Behandling af cancer ved anvendelse af humaniseret anti-EGFRvIII kimær antigenreceptor
PT2961831T (pt) * 2013-02-26 2020-10-12 Memorial Sloan Kettering Cancer Center Composições e métodos para imunoterapêutica
WO2016049024A2 (en) * 2014-09-24 2016-03-31 The Broad Institute Inc. Delivery, use and therapeutic applications of the crispr-cas systems and compositions for modeling competition of multiple cancer mutations in vivo
CN113713091A (zh) 2015-02-06 2021-11-30 新加坡国立大学 工程免疫细胞及其用途和生产方法
AU2017225733A1 (en) * 2016-03-04 2018-09-27 Novartis Ag Cells expressing multiple chimeric antigen receptor (CAR) molecules and uses therefore
SG11201903830TA (en) 2016-11-22 2019-05-30 Nat Univ Singapore Blockade of cd7 expression and chimeric antigen receptors for immunotherapy of t-cell malignancies
US20190038733A1 (en) * 2017-08-10 2019-02-07 National University Of Singapore T cell receptor-deficient chimeric antigen receptor t-cells and methods of use thereof
WO2019226946A1 (en) * 2018-05-23 2019-11-28 National University Of Singapore Chimeric antigen receptors and gene editing of cd2 for immunotherapy of t-cell malignancies
KR20210008408A (ko) * 2018-05-23 2021-01-21 싱가포르국립대학교 T-세포 악성 종양의 면역요법을 위한 cd2 표면 발현 및 키메라 항원 수용체의 발현에 대한 차단

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JP2025072367A (ja) 2025-05-09
EP3864052A4 (en) 2022-07-27
MA53877A (fr) 2022-01-19
JP2022512994A (ja) 2022-02-07
CN120519520A (zh) 2025-08-22
SG11202104334SA (en) 2021-05-28
AU2019378039A1 (en) 2021-05-27
US20210395779A1 (en) 2021-12-23
CA3119296A1 (en) 2020-05-22

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