KR20210067378A - Extraction method of functional protein material derived from black soldier fly - Google Patents

Abstract

The present invention relates to a method for extracting functional protein substances derived from black solder fly. In the present invention, functional protein derived from black solder fly, which does not show cytotoxicity as a natural product, can be obtained in high yield in a short period of time at low cost by extracting immune-related substances from dry powder of black solder fly using water. In addition, according to the improved extraction method, immune candidates can be obtained in a powder form through an additional formulation processing step, and is expected to be usefully utilized by being added to the existing feed at the correct concentration in the field.

Description

Extraction method of functional protein material derived from black soldier fly}

The present invention relates to a method for extracting functional protein substances derived from Dongae et al.

Until now, interest or research on the utilization of useful biological resources has been mainly focused on plant groups such as herbal medicines, and the interest in the use and development of useful insects worldwide was very low. Insects, which are highly adaptable to the environment, have the largest number of species on the planet and are distributed all over the world. These insect resources are increasing the value of use in various fields with the development of biotechnology. Insect resources are being applied in many fields, such as the production of useful substances, research tools for human diseases, the development of biological materials, use as medicinal insects, and eco-friendly agriculture.

Black Soldier Fly (Hermetia illucens (Diptera: Stratmyidae)) is native to North America and has a worldwide distribution, and is known to be well bred with organic waste such as food waste due to its wide range of food choices. Dongae, etc. live in places where organic waste is accumulated, such as livestock houses and food waste bins, and one adult on Dongae, etc. lays about 1000 eggs, and the larvae period is 15 to 20 days, ingesting livestock manure and food waste and decomposing it. . Research on the eco-friendly treatment of food waste and livestock waste, which are organic waste resources, and the recycling of organic resources, using the ecological characteristics of Dongae, etc. is being conducted. This organic waste treatment method using Dongae, etc. does not have secondary environmental pollution, and the by-products generated during this process are also highly safe, so it can be the most eco-friendly method among organic waste recycling methods. In addition, Dongae, which boasts excellent eating habits, has a good reproductive ability and is highly likely to be used continuously.

On the other hand, the homeless larvae and pupae that are generated after waste treatment using Dongae, etc. are mostly made of fat and protein, so it is known that the economic value of fish feed additives and livestock feed reaches 8,000 to 40,000 dollars per ton. In the case of pupa of Dongae, etc., it contains more than 50% of crude protein, and in the case of larvae, it is known as a good source of essential amino acids such as arginine, methionine, and lysine. The amino acid content of pupae and larvae of Dongae, etc. is higher than that of soybean meal and has a value comparable to that of fish meal, so various application methods have been reported for use as fish feed, poultry feed, and special feed for various animals.

As such, although the value of feed for Dongae has been proven in the previous studies, the use of larval feed for Dongae, etc. in the field is sluggish due to the lack of price competitiveness and the absence of measures to enhance added value. In particular, existing studies have only conducted general nutrient supply as a substitute for soybean meal or fish meal for larvae of Dongae, etc., so price competitiveness must be secured through improvement of manufacturing processes and systems. In addition, in order to enhance the added value of larvae in larvae, it is necessary to develop technology to use it as a supplementary feed by discovering functional substances in larvae and verifying the effect of feeding them. No, there are few studies on it.

Kim Seong-hyeon et al., 2013, “Nutritional Value of Dongae, etc. as Fish Feed,” Journal of the Korean Silkworm Society (former Journal of the Korean Silkworm Society), 51(2), 95-98.

Accordingly, the present inventors were studying the optimal extraction and separation method of a protein extract having functionality from larvae of Dongae et al., using water as a solvent and using a continuous centrifuge to separate candidate substances, and then efficiently using a spread drying method. By drying the material, it was confirmed that immune-related substances can be economically and efficiently obtained from dry powder of larvae such as Dongae, etc., and the present invention was completed.

Therefore, an object of the present invention is to extract (a) adding water to the powder of Dongae, etc.; (b) centrifuging the extracted protein extract of step (a); and (c) recovering and drying the supernatant from the resultant of step (b); to provide a method for extracting functional protein material derived from Dongae, characterized in that it comprises a.

Another object of the present invention is to provide a functional protein material derived from Dongae, characterized in that it is extracted by the above method, and uses thereof.

In order to achieve the above object, the present invention comprises the steps of (a) extracting by adding water to the powder such as Dongae; (b) centrifuging the extracted protein extract of step (a); and (c) recovering and drying the supernatant from the resultant of step (b); provides a method for extracting functional protein material derived from Dongae, characterized in that it comprises a.

In addition, the present invention provides a functional protein material derived from Dongae, characterized in that it is extracted by the above method.

In addition, the present invention provides an antibacterial composition comprising a functional protein material derived from Dongae, etc.

In addition, the present invention provides a feed composition comprising a functional protein material derived from Dongae, etc.

According to the present invention, functional protein derived from Dongae, etc., which does not show cytotoxicity as a natural product, can be obtained in high yield at low cost and in a short period of time by extracting immune-related substances from dry powder of Dongae, etc. using water. In addition, according to the improved extraction method, the immune candidate can be obtained in powder form through an additional formulation processing step, and it is expected that it will be usefully utilized by adding it to the existing feed at the correct concentration in the field.

1 is 100% EtOH (ethanol) + 1% AcOH (acetic acid) used for extraction using a mixed solvent of brown mealworm, slug and snail, and the amount of the extracted candidate material (% of the dried biomass, g / 100 g) is a diagram showing the results of measuring the yield of each immune candidate material.
Figure 2 is 100% EtOH, 50% EtOH and distilled water, respectively, used for extraction in Dongae, etc., and the amount of the extracted candidate material (% of the dried biomass, g / 100 g) to measure the yield according to the extraction solvent A diagram showing a result.
Figure 3 shows the results of the antimicrobial activity assay of the immune candidates derived from Dongae, etc. by solvent extract using Paper Disc. (A) 1g / 1mL distilled water 20 ul, (B) 1g / 1mL distilled water 1/10 dilution 20 ul, (C) Control (distilled water), (D) 1g / 1mL distilled water 20 ul 1/100 dilution, (E) 1 g / 1 mL distilled water 1/1000 dilution 20 ul.
Figure 4 is 100% distilled water, 100% distilled water + acetic acid mixed solvent, 50% ethanol, 50% ethanol + acetic acid mixed solvent, 100% ethanol, 100% ethanol + acetic acid mixed solvent, respectively, extracting the immune candidate substances derived from Dongae, etc. And, it is a diagram showing the cytotoxicity measurement result of the immune candidate material derived from Dongae et al.

Hereinafter, the present invention will be described in detail.

Terms not otherwise defined herein have meanings commonly used in the art to which the present invention pertains.

The present invention comprises the steps of (a) extracting by adding water to the powder such as Dongae; (b) centrifuging the extracted protein extract of step (a); and (c) recovering and drying the supernatant from the resultant of step (b); provides a method for extracting functional protein material derived from Dongae, characterized in that it comprises a.

In the present invention, the term “extract” refers to a portion extracted by the extraction solvent during the extraction process. That is, when it is separated into a precipitate and a supernatant by extraction, it is a part corresponding to the “supernatant”. In addition, the extract includes a fraction obtained by additionally fractionating the extract in a broad sense. That is, the extract according to the present invention includes not only those obtained by using an extraction solvent such as water (distilled water) or ethanol, but also those obtained by additionally applying a purification process here.

In the present invention, the Dongae and the like powder may be characterized in that the larva powder is the same. In one embodiment of the present invention, the larvae powder prepared by microwave drying is used, but as long as it is a larvae powder that can be extracted with water to extract a functional protein material, it can be used without limitation.

In the present invention, the step (a) may be to add 5 to 15 weight times of water to the powder in Dongae, etc., and extract under reflux at 50 to 70 ° C., preferably by adding 8 to 12 weight times of water to 55 to It may be to reflux extraction at 65 ℃, more preferably, it may be characterized in that the reflux extraction at 60 ℃ by adding 10 times by weight of water.

In addition, the step (a) may be characterized in that the extraction is performed after adjusting the pH to 9 by additionally adding caustic soda. The caustic soda may be caustic soda for food additives, but is not limited thereto.

In one embodiment of the present invention, the larvae powder prepared through microwave drying is refluxed in an extraction tank while the pH is adjusted to 9 using water and caustic soda for food additives in an amount of 10 times 60°C, and then the primary extraction is performed. did.

In the present invention, the centrifugation of step (b) may be performed under the conditions of 6000 to 7000 rpm, preferably about 6,400 rpm (about 10,000 g), but after the first extraction using water, the centrifugation is performed to immunize As long as it is a condition capable of efficiently separating the candidate material, it may be applied without being limited to the above condition.

In the present invention, the drying of step (c) may be performed at 30 to 50 cmHg, 80 to 90 ° C. conditions with a spread dryer, preferably 35 to 45 cmHg, 83 to 87 ° C. conditions, and more Preferably it can be carried out at 40 cmHg, 85 ℃ conditions.

In one embodiment of the present invention, the firstly extracted protein extract is heat-treated for 2 hours to remove tyrosinase activity, centrifuged at 6,400 rpm, and the supernatant is recovered, using a spread dryer at 40 cmHg, 85 ° C. It was dried at 48 hours/kg to obtain a functional protein material derived from Dongae, etc. according to the present invention.

In addition, the present invention provides a functional protein material derived from Dongae, characterized in that it is extracted by the above method.

In addition, the present invention provides an antibacterial composition comprising a functional protein material derived from Dongae, etc.

The antimicrobial composition preferably has antimicrobial activity against Gram-positive bacteria or Gram-negative bacteria.

The antibacterial composition is preferably selected from the group consisting of pharmaceutical compositions, health functional foods, cosmetic compositions, food additives, additives for animal feed, detergents and cleaners.

The functional protein material derived from Dongae, etc., obtained according to the extraction method of the present invention, exhibits strong antibacterial activity, and is a material for pharmaceutical compositions, health functional foods, foods, additives for animal feed, and various cleaning agents related to the prevention or treatment of pathogenic bacterial infections. can be used as

In a preferred embodiment, the antimicrobial composition of the present invention can be applied as a pharmaceutical composition.

The pharmaceutical composition is formulated in various forms, such as oral formulations such as powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, and injections of sterile injection solutions, according to conventional methods for each purpose of use. It can be used, and it can be administered through various routes including oral administration or intravenous, intraperitoneal, subcutaneous, rectal, topical administration, and the like. The pharmaceutical composition may further include a carrier, excipient, or diluent, and examples of suitable carriers, excipients or diluents that may be included include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, Starch, gum acacia, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, amorphous cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil and the like. In addition, the pharmaceutical composition of the present invention may further include a filler, an anti-agglomeration agent, a lubricant, a wetting agent, a flavoring agent, an emulsifier, a preservative, and the like.

The pharmaceutical composition of the present invention is administered in a pharmaceutically effective amount. In the present invention, "pharmaceutically effective amount" means an amount sufficient to treat a disease with a reasonable benefit/risk ratio applicable to medical treatment, and the effective dose level is determined by the type, severity, activity of the drug, and the type of disease in the patient; Sensitivity to the drug, time of administration, route of administration and rate of excretion, duration of treatment, factors including concomitant drugs, and other factors well known in the medical field. The pharmaceutical composition of the present invention may be administered as an individual therapeutic agent or may be administered in combination with other therapeutic agents, may be administered sequentially or simultaneously with conventional therapeutic agents, and may be administered single or multiple. In consideration of all of the above factors, it is important to administer an amount that can obtain the maximum effect with a minimum amount without side effects, which can be easily determined by those skilled in the art.

The effective amount of the component having antibacterial activity in the pharmaceutical composition of the present invention may vary depending on the age, sex, and weight of the patient, and generally 1 to 5,000 mg per body weight, preferably 100 to 3,000 mg per body weight, is administered daily or every other day Or it can be administered in divided doses 1 to 3 times a day. However, since it may increase or decrease depending on the route of administration, the severity of the disease, sex, weight, age, etc., the dosage is not intended to limit the scope of the present invention in any way. The pharmaceutical composition of the present invention may be administered to a subject through various routes. Any mode of administration can be envisaged, for example, by oral, rectal or intravenous, intramuscular, subcutaneous, intrauterine dural or intra-cerebroventricular injection. In the present invention, "administration" means providing a predetermined substance to a patient by any suitable method, and the administration route of the pharmaceutical composition of the present invention is oral or parenteral through all general routes as long as it can reach the target tissue. It can be administered orally. In addition, the composition of the present invention may be administered using any device capable of delivering an active ingredient to a target cell. In the present invention, "subject" includes, but is not particularly limited to, for example, humans, monkeys, cattle, horses, sheep, pigs, chickens, turkeys, quails, cats, dogs, mice, rats, rabbits or guinea pigs. .

In a preferred embodiment, the antimicrobial composition of the present invention can be applied as a dietary supplement.

The health functional food of the present invention can be used in various ways, such as effective food and beverages that can lower the possibility of infection from a subject with a high risk of infection with pathogenic microorganisms. Foods containing the active ingredient having excellent antibacterial activity of the present invention include, for example, various foods, beverages, gum, tea, vitamin complexes, health supplements, etc., in the form of powders, granules, tablets, capsules or beverages. can be used as The active ingredient of the present invention can generally be added in 0.01 to 15% by weight of the total food weight, and the health beverage composition can be added in a ratio of 0.02 to 10 g, preferably 0.3 to 1 g, based on 100 ml. In addition to containing the above compound as an essential ingredient in the proportion indicated, the health functional food of the present invention may contain food pharmaceutically acceptable food supplement additives, such as natural carbohydrates and various flavoring agents, as additional ingredients. Examples of the natural carbohydrate include monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, and common sugars such as polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As the flavoring agent, thaumatin, rebaudioside A, glycyrrhizin, saccharin, aspartame, and the like may be used. The ratio of the flavoring agent is generally about 1 to 20 g, preferably about 5 to 12 g per 100 ml of the health functional food of the present invention. In addition to the above, the health functional food of the present invention includes various nutrients, vitamins, minerals, flavoring agents such as synthetic and natural flavoring agents, colorants and thickeners, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloids It may contain thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonation agents used in carbonated beverages, and the like. In addition, the health functional food of the present invention may contain natural fruit juice, fruit juice for the production of fruit juice drinks, vegetable drinks, and the like. These components may be used independently or in combination. The proportion of these additives is generally selected in the range of 0.01 to about 20 parts by weight per 100 parts by weight of the active fraction of the present invention.

In a preferred embodiment, the antimicrobial composition of the present invention can be applied as an additive for food or animal feed.

The additive is added to impart preservation to the food or animal feed used, and since the antibacterial composition is not harmful as a natural ingredient, it can be safely applied to food or animal feed.

The additive may further include additional other additives appropriately selected according to the purpose and purpose of use, and may be used by mixing with other active ingredients such as commonly used additives or additives such as pigments, surfactants, and preservatives. . The additive may be manufactured by powdering, granulating, tableting, or liquefying according to the purpose and use thereof, and a conventional method for packaging may be used for commercialization.

In addition, the amount of the antimicrobial composition used among the additives may be appropriately used depending on the purpose of use, application form, application site, application target article, etc., for example, may include 0.01 parts by weight to 50 parts by weight based on the total composition, but the antibacterial composition The content of the composition is not limited thereto. Since the antibacterial composition of the present invention is a natural component and is not harmful to the human body, it can be used in various amounts as long as the application purpose can be achieved due to the characteristics of the product.

In a preferred embodiment, the present invention can be applied as an antibacterial composition for use as a cleaning agent.

The cleaning agent may be preferably a detergent for clothes, a dishwashing agent, a food cleaner, and a household appliance cleaner. Since the antibacterial composition is a natural component, it is not harmful to the human body, and more preferably, it can be used for the purpose of washing various household items including kitchenware or food washing or for imparting antibacterial properties.

The detergent may further include an additive selected appropriately according to the purpose and purpose of its use, and may be used by mixing with other active ingredients such as commonly used detergents or additives such as pigments, surfactants, and preservatives. The detergent may be manufactured by powdering, granulating, tableting or liquefying according to the purpose and use thereof, and a conventional method for packaging may be used for productization.

In addition, the amount of the antibacterial composition used in the cleaning agent may be appropriately used depending on the purpose of use, application form, application site, application target article, etc., for example, it may include 0.01 parts by weight to 50 parts by weight based on the total composition, but the antibacterial composition The content of the composition is not limited thereto. Since the antibacterial composition of the present invention is a natural component and is not harmful to the human body, it can be used in various amounts as long as the application purpose can be achieved due to the characteristics of the product.

In a preferred embodiment, the present invention can be applied as an antibacterial composition for use as a cleaning agent.

The cleaning agent may further include additional additives appropriately selected according to the purpose and purpose of use, and may be used by mixing with other active ingredients such as commonly used additives or additives such as pigments, surfactants, and preservatives. . The cleaning agent may be manufactured by powdering, granulating, tableting, or liquefying depending on the purpose and use thereof, but in the case of a liquid type, convenient use will be possible.

In addition, the amount of the antibacterial composition used in the cleaning agent may be appropriately used depending on the purpose of use, application form, application site, etc., for example, it may include 0.01 parts by weight to 50 parts by weight based on the total composition, but the content of the antibacterial composition is The present invention is not limited thereto. Since the antibacterial composition of the present invention is a natural component and is not harmful to the human body, it can be used in various amounts as long as the application purpose can be achieved due to the characteristics of the product.

In addition, the present invention provides a feed composition comprising a functional protein material derived from Dongae, etc.

The feed composition includes echinoderms, crustaceans, mollusks, fish, amphibians, reptiles, birds, mammals, preferably echinoderms such as sea urchins or sea cucumbers, arthropods including crustaceans such as crabs, shrimps and lobsters, cephalopods , molluscs such as gastropods or bivalves, fish such as red sea bream, sea bream, cod, flounder, halibut, birds including poultry such as pheasants or chickens, or animals including mammals such as pigs, cattle, goats, etc. .

The feed composition may further include grains, vegetable protein feed, animal protein feed, sugar or dairy products in the functional protein material derived from Dongae, etc. of the present invention. The grain may be specifically pulverized or crushed wheat, oats, barley, corn and rice, and the vegetable protein feed may specifically include rapeseed, soybean and sunflower as main components, and the animal protein feed is specifically may be blood meal, meat meal, bone meal, and fish meal, and the sugar or dairy product may specifically be a dry component composed of various types of powdered milk and whey powder.

The feed composition may further be used together with ingredients such as nutritional supplements, digestion and absorption enhancers, vocal tract promoters or disease prevention agents.

The functional protein material derived from Dongae, etc. included in the feed composition of the present invention may vary depending on the purpose and conditions of use of the feed, for example, 0.1 g to 100 g of the functional protein material derived from Dongae, etc. based on 1 kg of the final feed. may be included.

In addition, the feed composition may be prepared as a hard viscous granulated or granular material depending on the degree of pulverization of the ingredients, and the composition may be supplied as a mesh or formed into a desired separated shape for further processing and packaging, and storage For ease of storage, it may be subjected to pelletization, expansion or extrusion process, and preferably excess water may be removed by drying.

In one embodiment of the present invention, the functional protein material derived from Dongae, etc. obtained by the extraction method of the present invention by confirming the antibacterial activity of the functional protein material derived from Dongae, etc. and further confirming that there is no cytotoxicity of the material is used for antibacterial use. It was confirmed that it can be usefully used as a composition or a feed composition. In addition, the extraction method according to the present invention has the advantage that it is very easy to use by adding it to an existing feed at an accurate concentration because the functional protein material derived from Dongae, etc. is powdered through an additional formulation processing step.

Hereinafter, the present invention will be described in more detail through examples. These examples are only for illustrating the present invention in more detail, and it will be apparent to those skilled in the art that the scope of the present invention is not limited by these examples according to the gist of the present invention. .

Example One. in a fraternity Confirmation of extractability of larval powder

The extraction yield of immune candidates for feeding larvae to brown mealworms, slugs and larvae was measured. As a solvent, 100% EtOH (ethanol) + 1% acetic acid was used, and the yield of the immune candidate material from each insect larva was determined from the amount of the extracted candidate material (% of the dried biomass, g/100 g). The results are shown in Table 1 and FIG. 1 below.

As shown in Table 1 and FIG. 1, the amount of the candidate substance extracted per 100 g is 12.93 g, which is less than 23.13 g of brown mealworm that is used for food, but higher than 10.34 g of white spot larvae (slug). It was confirmed that the yield was shown. Through the above results, it was confirmed the possibility of extracting candidate substances of larvae powder such as Dongae.

Example 2. in a fraternity Measurement of extraction yield for each solvent of the derived immune candidate material

Water (distilled water), 50% EtOH (ethanol), and 100% EtOH (ethanol) were used to measure the extraction yield of the immune candidates for Dongae et al. by solvent. The results are shown in Table 2 and FIG. 2 below.

As shown in Table 2 and Figure 2, when the candidate material was extracted using water (distilled water), 100 g to 21.9 g of the immune candidate material could be extracted, and when extracted using 50% EtOH and 100% EtOH, It was confirmed that the extraction efficiencies of 20.6 and 23.9 g, respectively, were shown. Through the above results, it was confirmed that even if the candidate material was extracted using water instead of ethanol, the difference in yield did not appear much, so there was no problem for industrial use.

Example 3. in a fraternity Measurement of antimicrobial activity by solvent of derived immune candidates

3-1. Antibacterial activity by solvent

Antibacterial activity of immune candidates derived from Dongae, etc. extracted using water (distilled water), 50% EtOH and 100% EtOH was measured, and the results are shown in Table 3 below. The figures in the table indicate the diameter (cm) of the inhibition zone (clear zone) measured according to the Paper disc test, and the following test strains were used for the antimicrobial activity assay: Bacillus subtilis subsp. Spizizenii, KACC 14741 (Gram-positive); Escherichia coli , KCCM 11234 (Gram-negative); Salmonella gallinarum (Gram-negative); Listeria monocytogenes , KCCM 4315 (Gram-positive)

As shown in Table 3, it was confirmed that 50% EtOH extraction showed antibacterial activity against Bacillus subtilis , Listeria monocytogenes and Salmonella gallinarum. Listeria for water (distilled water) extraction Monocytogenes showed antibacterial activity, and it was confirmed that 100% EtOH extraction showed antibacterial activity against Bacillus subtilis.

3-2. Antimicrobial activity by concentration according to dilution of solvent extract

Next, the antibacterial activity according to the dilution of the solvent extract of the immune candidate substance was confirmed. The results are shown in Table 4 and FIG. 3 below.

As shown in Table 4 and Figure 3, in a 1/2-fold dilution of 50% EtOH extract also Bacillus subtilis , Listeria It was confirmed that the antibacterial effect appeared in monocytogenes and Salmonella gallinarum.

Example 4. in a fraternity Confirmation of cytotoxicity of derived immune candidates

Cytotoxicity was measured to confirm the possibility of feed application of the immune candidate material derived from Dongae, etc., whose antimicrobial activity was confirmed. For cytotoxicity analysis, cytotoxicity in cultured cells was analyzed using NO/MTS assay. The results are shown in FIG. 4 .

As shown in FIG. 4 , cytotoxicity was not observed in the 100% water (DW) extract, the water (DW)+3% AcOH (acetic acid) extract, and the 50% EtOH (ethanol)+3% AcOH (acetic acid) extract. On the other hand, extracts of 50% EtOH (ethanol), 100% EtOH (ethanol) and 100% EtOH (ethanol) + 3% AcOH (acetic acid) showed cytotoxicity.

Through the above results, it was confirmed that when 100% of water (distilled water) was used to extract the immune candidate material derived from Dongae, etc., it could be extracted at a low cost without showing cytotoxicity.

Example 5. in a fraternity of derived immune candidates formulation processing technology development

The existing processing method used a method of dissolving dongae powder in water, collecting the supernatant using a centrifugal separator, filtering it, and then drying the candidate material by freeze-drying or hot air drying. On the other hand, in order to extract functional protein extracts from larvae of Dongae, etc. in an optimized method, the present inventors separated candidate substances using a continuous centrifuge and then spread drying method to efficiently dry candidate substances. processing technology was developed.

Specifically, the larvae powder prepared through microwave drying was refluxed in an extraction tank and the pH was adjusted to 9 using 10 times the amount of water at 60°C and caustic soda for food additives, and then the primary extraction was performed. After that, the first extracted protein extract was heat treated for 2 hours to remove tyrosinase activity, centrifuged at 6,400 rpm, and the supernatant was recovered, using a spread dryer at 40 cmHg and 85° C. for 48 hours/kg dried with Table 5 below shows the difference in effectiveness of the existing formulation processing technology and the improvement process according to the present invention.

division conventional processing improvement process yield 15% 32% Purification period 7 days 2 days shape gel powdery Characteristic Dissolve and use for use in existing feed Can be used immediately by adding it to existing livestock feed

As shown in Table 5, when the immune candidate material derived from Dongae, etc. was isolated and formulated by the extraction method according to the present invention, the yield was more than doubled to 32%, and it was confirmed that the purification period was also shortened from 7 days to 2 days. . The immune candidate substances in Dongae et al. are in powder form, so it is expected that they can be calculated and utilized at an accurate concentration in existing feeds.

Through the above results, the extraction method of the present invention can obtain an extract derived from larvae of Dongae, etc. having excellent antibacterial activity, and the extract can be formulated in various forms, which is expected to reduce production costs. Big. In addition, it was confirmed that the yield is improved and the purification period shortened, so that it can be used economically in terms of time and cost when it is formulated after isolating and formulating an immune candidate material derived from Dongae, etc. according to the optimized extraction method. .

As described above in detail a specific part of the present invention, for those of ordinary skill in the art, this specific description is only a preferred embodiment, and it is clear that the scope of the present invention is not limited thereto. Accordingly, the substantial scope of the present invention will be defined by the appended claims and their equivalents.

Claims (10)

(a) extracting by adding water to the powder such as Dongae;
(b) centrifuging the extracted protein extract of step (a); and
(c) recovering the supernatant from the resultant of step (b) and drying the method; The method for extracting functional protein material derived from Dongae, according to claim 1, wherein the powder of Dongae, etc. is a larva powder of Dongae, etc. The method for extracting functional protein material derived from Dongae, according to claim 1, wherein the powder of Dongae, etc. is prepared through microwave drying. The method of claim 1, wherein in step (a), 5 to 15 times by weight of water is added to the powder of Dongae, etc., and the extraction is performed under reflux at 50 to 70°C. According to claim 1, wherein step (a) is characterized in that the extraction is performed after adjusting the pH to 9 by adding caustic soda. The method of claim 1, wherein the centrifugation of step (b) is performed under the conditions of 6000 to 7000 rpm. The method of claim 1, wherein the drying in step (c) is performed at 30 to 50 cmHg and 80 to 90° C. with a spread dryer. A functional protein material derived from Dongae, characterized in that it is extracted by the method of any one of claims 1 to 7. An antibacterial composition comprising a functional protein material derived from Dongae of claim 8. A feed composition comprising a functional protein material derived from Dongae and the like of claim 8.

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