KR102380290B1 - Composition Comprising supermealworm extract for preventing or treating Obesity - Google Patents

Abstract

The present invention relates to a composition for preventing or treating obesity, comprising an American mealworm larva extract. The American mealworm larva extract according to the present invention has no side effects, has excellent safety, and has anti-obesity efficacy, so it can be used as a preventive or therapeutic agent for obesity or obesity-related diseases, health food supplements and feed compositions.

Description

A composition for preventing or treating obesity comprising an American mealworm larva extract {Composition Comprising supermealworm extract for preventing or treating Obesity}

The present invention relates to a composition for preventing or treating obesity, comprising an American mealworm larva extract.

Obesity is a phenomenon in which the intake of calories exceeds the energy required for physical activity due to the excessive accumulation of fat in the body due to increased fat intake. It is reported to act as an important factor that increases the frequency of chronic diseases such as diabetes and liver cirrhosis.

In addition, although the exact mechanism of obesity is a complex syndrome, weight loss in obesity is very important for effectively reducing the risk of type 2 diabetes in improving insulin resistance, glucose tolerance and lipoprotein disorders.

However, in the case of chronic diseases such as obesity and diabetes, it is not easy to treat because it is often caused by lifestyle and genetic predisposition. Accordingly, there are methods for weight loss, such as exercise, diet, drug administration, and surgery, and the double diet is the most fundamental method for preventing and treating obesity.

Accordingly, many studies are being conducted to find effective therapeutic agents from foods or natural products for the prevention and treatment of obesity.

As related prior art, Korean Patent Application Laid-Open No. 10-2003-0001659 (extract of white-spotted flower radish and its use) and Korean Patent Application Laid-Open No. 10-2012-0069041 (Anti-obesity composition containing fermented radish bark as an active ingredient) are related. is disclosed. Recently, as competition for securing biological resources through the search and conservation of insect resources is getting fiercer, research on their value evaluation and use and development is being conducted very actively.

American Mealworm ( Zophobas) atratus ) is an insect belonging to the class Insects, Coleoptera, Meuraceae, and Melanoptera. The larvae of American Mealworm are known to grow up to 50-60 mm, so they are also called superworms or supermealworms. The larva of the American mealworm is relatively larger than the larva of the brown mealworm (mealworm) and has been mainly used as a protein supply source for pets such as lizards, turtles, frogs, salamanders, birds, and koi.

So far, previous studies related to mealworms have been molitor ), and studies on the larvae of the American mealworm larvae are lacking. The present inventors completed the present invention by confirming that the larvae extract of A. egret larvae have excellent anti-obesity effect while studying various physiological activities using the Melancholic larvae extract.

The problem to be solved by the present invention is to provide a pharmaceutical composition for preventing or treating obesity comprising an American mealworm larva extract.

Another object to be solved by the present invention is to provide a food composition for preventing or improving obesity comprising an American mealworm larva extract.

Another object to be solved by the present invention is to provide a feed composition for preventing or improving obesity comprising an American mealworm larva extract.

Another object to be solved by the present invention is to provide a health functional food for preventing or improving obesity, including an American mealworm larva extract.

In order to achieve the above object, the present invention provides a pharmaceutical composition for preventing or treating obesity, comprising an American mealworm ( Zophobas atratus ) larval extract as an active ingredient.

According to the present invention, the larvae extract of Melancholy larvae is characterized in that _{the larvae of Melancholy of America are extracted using water, C 1-4 alcohol} , or a mixed solution thereof as a solvent.

Water, C _1-4 alcohol, or a mixed solution thereof used in the preparation of the larvae extract of A. kingfisher larvae may be used in an amount of ₁ to 40 times the volume (1 to 40 liters based on 1 kg) based on the weight of the larvae of A. larvae, preferably can use 5 to 40 times the volume. The extraction conditions of the American mealworm larvae extract may be 1 minute to 48 hours at 20 ~ 100 ℃. The above process can be repeated 1 to 4 times.

According to the present invention, the American mealworm larvae extract can be extracted using the American mealworm larvae additionally performed at least one step selected from the group consisting of fasting, freezing, washing, autoclaving and freeze-drying before extraction. .

According to the present invention, the American mealworm larvae can be used after fasting for 12 to 48 hours before use, then frozen, thawed, washed, sterilized, and then dried and pulverized American mealworm larvae. This process is preferable because it can improve the storage stability of the American mealworm larva extract and increase the content of the active ingredient.

According to the present invention, the C _1-4 alcohol may be selected from the group consisting of methanol, ethanol, _propanol , isopropanol, butanol and isobutanol, preferably ethanol.

According to the present invention, the American mealworm larva extract may be extracted using 60 to 80% by volume aqueous ethanol solution as a solvent, and more preferably, extracted using 70% by volume aqueous ethanol solution as a solvent.

According to the present invention, the American mealworm larva extract can be used as it is filtered, sterilized or concentrated, and in order to increase activity, n-hexane, methylene chloride, acetone, chloroform, ethyl acetate and n-butanol from the group consisting of It may be further fractionated using one or more selected solvents to prepare fractions, or the active ingredient may be purified and used using various purification methods such as silica gel column chromatography or high performance liquid chromatography.

The American mealworm larva extract according to the present invention can inhibit adipogenesis in vivo, and can inhibit protein signals related to adipogenesis. In this regard, diseases related to adipogenesis, for example, obesity including complications of obesity, for example, hyperlipidemia, fatty liver, visceral obesity, abdominal obesity, systemic obesity, partial obesity, and prevention of diseases selected from the group consisting of giant lipoma or It can effectively act as a therapeutic pharmaceutical composition.

The American mealworm larva extract may be added to the pharmaceutical composition of the present invention in an amount of 0.001 to 95% by weight. The pharmaceutical composition may be formulated in the form of powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, etc., external preparations, suppositories and sterile injection solutions according to conventional methods, respectively. Carriers, excipients and diluents that may be included in the pharmaceutical composition include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia gum, alginate, gelatin, calcium phosphate, calcium silicate, cellulose , methylcellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. In the case of formulation, it is prepared using commonly used diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrants, and surfactants. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, etc., and such solid preparations include at least one excipient in the extract of the present invention, for example, starch, calcium carbonate, sucrose or lactose, It is prepared by mixing gelatin, etc. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Liquid formulations for oral use include suspensions, solutions, emulsions, syrups, etc. In addition to water and liquid paraffin, which are commonly used simple diluents, various excipients, for example, wetting agents, sweeteners, fragrances, preservatives, etc. may be included. . Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Non-aqueous solvents and suspending agents include propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable esters such as ethyl oleate. As the base of the suppository, witepsol, macrogol, tween 61, cacao butter, laurin, glycerogelatin, etc. can be used.

The dosage of the pharmaceutical composition of the present invention will vary depending on the age, sex, and weight of the subject to be treated, the specific disease or pathological condition to be treated, the severity of the disease or pathological condition, the route of administration, and the judgment of the prescriber. Dosage determination based on these factors is within the level of one of ordinary skill in the art, and generally dosages range from 0.01 mg/kg/day to approximately 2000 mg/kg/day. A more preferred dosage is 1 mg/kg/day to 500 mg/kg/day. Administration may be administered once a day, or may be administered in several divided doses. The above dosage does not limit the scope of the present invention in any way.

The pharmaceutical composition of the present invention may be administered to mammals such as mice, livestock, and humans by various routes. Any mode of administration can be envisaged, for example, by oral, rectal or intravenous, intramuscular, subcutaneous, intrauterine dural or intracerebrovascular injection. Since the extract of the present invention has almost no toxicity and side effects, it is a drug that can be safely used even when taken for a long period of time for the purpose of prevention.

The present invention provides an extract of Melancholy larvae for the treatment of obesity.

The present invention provides for the use of an extract of Melancholy larvae in the manufacture of a medicament for the treatment of obesity.

The present invention provides a method for treating obesity, comprising the step of administering to a subject in need thereof, a therapeutically effective amount of the American mealworm larvae extract.

In addition, the present invention is American Meal ( Zophobas) atratus ) Provides a food composition for preventing or improving obesity, including the larvae extract.

According to the present invention, the American mealworm larva extract may be an extract of the American mealworm larvae using water, C _1-4 alcohol, or a mixed solution thereof as a solvent, _{and preferably 60 to 80% by volume C 1-4 alcohol aqueous} solution , more preferably 60 to 80% by volume ethanol aqueous solution, more preferably 70% by volume ethanol aqueous solution.

According to the present invention, the American mealworm ( Zophobas atratus ) Provides a health functional food for preventing or improving obesity, including larvae extract.

According to the present invention, the health functional food may be for preventing or improving any one symptom selected from the group consisting of obesity, such as hyperlipidemia, fatty liver, visceral obesity, abdominal obesity, systemic obesity, partial obesity, and giant lipoma. .

The food composition of the present invention may contain conventional food additives, and the suitability as the "food additive" is determined according to the general rules and general test methods of food additives approved by the Ministry of Food and Drug Safety, unless otherwise specified. It is judged according to the standards and standards related to the item.

The term "health functional food" means a food manufactured and processed using raw materials or ingredients useful for the human body in accordance with the Health Functional Food Act, and "functionality" refers to the structure and function of the human body. It refers to ingestion for the purpose of obtaining useful effects for health purposes such as regulating nutrients or physiological effects.

The items listed in the "Food Additives Code" include, for example, chemical compounds such as ketones, glycine, potassium citrate, nicotinic acid, and cinnamic acid; Mixed preparations such as sodium L-glutamate preparation, noodle-added alkali agent, preservative agent, and tar color agent can be mentioned.

The food composition of the present invention may contain 0.01 to 95% by weight, preferably 1 to 80% by weight of the American mealworm larvae extract based on the total weight of the composition.

In addition, the food composition of the present invention can be manufactured and processed in the form of tablets, capsules, powders, granules, liquids, pills, and the like.

For example, the health functional food in the form of tablets is granulated by a conventional method by granulating a mixture with an American mealworm larva extract, excipients, binders, disintegrants, and other additives, and then compression molding by putting a lubricant, etc.; The mixture can be directly compression molded. In addition, the health functional food in the form of tablets may contain a mating agent, etc., if necessary, and may be coated with a suitable skinning agent if necessary.

Among the health functional foods in the form of capsules, hard capsules can be prepared by filling conventional hard capsules with a mixture with additives, such as American mealworm larvae extract and excipients, or their granules or coated granules. It can be prepared by filling a mixture with an excipient such as an American mealworm larva extract and excipients into a capsule base such as gelatin. The soft capsule formulation may contain a plasticizer such as glycerin or sorbitol, a colorant, a preservative, and the like, if necessary.

A health functional food in the form of a pill can be prepared by molding a mixture of Mealworm larvae extract, excipients, binders, disintegrants, etc. in an appropriate way, and if necessary, the skin is coated with sucrose or other suitable skinning agent, or starch, talc, or You can also dress the gown with a suitable material.

The health functional food in the form of granules can be prepared in a granular form by an appropriate method by mixing a mixture of Mealworm larvae extract, excipients, binders, disintegrants, etc. For health functional foods in granular form, use No. 12 (1680 μm), No. 14 (1410 μm), and No. 45 (350 μm) sieves to pass all the sieves in the next particle size test, and the remaining amount in No. 14 sieves 5.0% or less and passing through No. 45 may be less than 15.0% of the total amount.

The term definitions for the excipients, binders, disintegrants, lubricants, flavoring agents, flavoring agents, etc. are described in documents known in the art and include those having the same or similar functions (Explanation of the Korean Pharmacopoeia, Moonseongsa, Korea) Association of Colleges of Pharmacy, 5th ed., p33-48, 1989).

There is no particular limitation on the type of the food. Examples of the food to which the American mealworm larva extract of the present invention can be added include beverages, gums, vitamin complexes, drinks, and the like, and include all health functional foods in a conventional sense.

The food composition may contain food supplement additives, and the food products include, for example, beverages, gum, tea, vitamin complexes, health supplements, etc., capsules, tablets, powders, granules, liquids, pills, slices, pastes. It can be used in the form of phases, syrups, gels, beverages, jellies or vines.

The content of the American mealworm larva extract may be contained in an amount of 0.01 to 15% by weight based on the total weight of the health functional food, and in the case of a health drink composition, 0.02 to 10 g, preferably 0.3 to 1, based on a total of 100 g g may contain.

The food supplement additives include food additives conventional in the art, for example, flavoring agents, flavoring agents, coloring agents, fillers, stabilizers, and the like. In addition, the health drink composition is an essential ingredient in the indicated ratio, and there is no particular limitation on the ingredients added other than the American mealworm larvae extract. there is. Examples of the natural carbohydrate include monosaccharides such as glucose, fructose and the like; disaccharides such as maltose, sucrose and the like; polysaccharides such as dextrins, cyclodextrins; and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those described above, natural flavoring agents (taumatine, stevia extract (eg rebaudioside A, glycyrrhizin, etc.)) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. there is.

In addition to the above, the food composition or health functional food containing the American mealworm larva extract is a flavoring agent such as various nutrients, vitamins, minerals (electrolytes), synthetic flavoring agents and natural flavoring agents, coloring agents and thickening agents (cheese, chocolate, etc.) ), pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like.

In addition, the present invention provides a feed composition for the prevention or improvement of obesity, comprising an American mealworm larva extract.

The American mealworm larva extract may be an extract of the American mealworm larvae using water, C _1-4 alcohol, or a mixed solution thereof as a solvent, preferably 60 to 80% by volume _{C 1-4 alcohol} aqueous solution, more preferably Preferably, it may be 60 to 80% by volume ethanol aqueous solution, more preferably 70% by volume ethanol aqueous solution.

In the present invention, the feed may be a feed for reptiles, fish, birds or mammals, and preferably, it is defined in Article 2 No. 1 of the Livestock Act and Article 2 of the Enforcement Rule of the Act, and the wild habits are acclimatized to breeding. It can be feed for livestock or aquatic organisms that can contribute to the increase of farm household income. The livestock may include cattle, horses, mules, donkeys, goats, goats, sheep, deer, pigs, rabbits, poultry, etc., and the poultry includes chickens, turkeys, ducks, ostriches, geese, quails, etc., preferably chickens, If it is suitable for breeding and obtaining livestock products, it is not limited thereto. The above "livestock products" are meat, milk, eggs, honey and their processed products, raw hides (including raw fur), raw wool, and other livestock products, which are defined by Article 2, 3 of the Livestock Act, as determined by the Ordinance of the Ministry of Agriculture and Forestry. means that In addition, it may be a dog, a cat, etc., including companion animals, but is not limited thereto.

The term "feed" in the present invention means any natural or artificial diet, meal, etc., or a component of said meal, intended for or suitable for being eaten, consumed, and digested by an animal. In one aspect, the composition for feed containing the American mealworm larva extract of the present invention may include a concentrated feed, roughage and / or special feed.

In the enriched feed, seed fruits containing grains such as wheat, oats, and corn, bran containing rice bran, bran, barley bran, etc. Fish-soluble, meat powder, which is a concentrated product of fish meal, fish waste, and fresh liquids obtained from fish meal, fish waste, and residual starch, which is the main component of starch residue, which is the remainder of starch residue obtained by subtracting starch from sweet potatoes and potatoes. , animal feed such as dried whey, yeast, chlorella, seaweed, etc., which are dried whey, which is the remainder of the production of casein from milk, cheese, and casein from skim milk.

For roughage, raw grass feed such as wild grasses, grasses, and green cuttings, turnips for feed, beets for feed, root vegetables such as ruther beargers, a type of turnip, raw herbs, green crops, grains, etc. are placed in a silo. There are silage, which is stored feed fermented with lactic acid, wild grass, hay dried after cutting grass, straw for breeding crops, and leaves from legumes. Special feed includes mineral feed such as oyster shells and rock salt, urea feed such as urea or its derivative diureide isobutane, and supplementation of ingredients that are likely to be lacking when only natural feed ingredients are mixed, or added to formulated feed to increase feed storage. There are feed additives, which are substances added in trace amounts.

The anti-obesity composition comprising the American mealworm larvae extract according to the present invention as an active ingredient has no side effects, has excellent safety, and has anti-obesity efficacy, thereby preventing or treating obesity or obesity-related diseases, health food supplements and It can be used as a feed composition.

Figure 1 is a result of assaying the cytotoxicity of the larvae extract of American Mealworm larvae according to an embodiment of the present invention.
Figure 2 is the result of confirming the effect of inhibiting adipocyte differentiation of the American mealworm larva extract according to an embodiment of the present invention by oil red O staining method.
3 is a result of confirming the effect of inhibiting the expression of adipocyte differentiation-related protein of the American mealworm larva extract according to an embodiment of the present invention.

Hereinafter, preferred examples are presented to help the understanding of the present invention, but the following examples are merely illustrative of the present invention, and it will be apparent to those skilled in the art that various changes and modifications are possible within the scope and spirit of the present invention, It goes without saying that such variations and modifications fall within the scope of the appended claims.

<Example 1. Preparation of American Mealworm Larvae Extract>

American Mealworm ( Zophobas) atratus ) larvae were separated from feces using a sieve for 2 days and washed with running water. After spreading the non-woven fabric in the container of the high-temperature/high-pressure sterilizer, the American mealworm larvae were put, and sterilized at 115° C. and 1 kgf/cm 2 for 10 to 15 minutes. It was stored in a cryogenic freezer for 24 hours, and then freeze-dried within 60-72 hours and then pulverized with a multifunctional grinder and used in the experiment. 1 g of the powder of American king mealworm larvae collected in this way was put in 10 ml 70% ethanol aqueous solution, and after vortexing, cultured for 24 hours in a shaking incubator at 37°C, centrifuged at 4°C at 15,000 rpm for 20 minutes, and the supernatant was It was collected and filtered and sterilized with a 0.45 μm syringe filter. Thereafter, by drying with a rotary vacuum concentrator (speedvac), the larvae extract of the American mealworm of the present invention was prepared.

<Test Example 1. Cell viability test>

Cell culture and differentiation induction

To confirm the anti-obesity effect, 3T3-L1 preadipocytes (Korea Cell Line Bank) were incubated at 37 ° C., 5% CO ₂ with antibiotics (100 units/mL penicillin-streptomycin) and 10% calf serum (CS). ) containing Dulbecco's Modified Eagle Medium (DMEM) was used for incubation. For cell differentiation, 1×10 ⁶ cells/well were aliquoted in a 6-well plate, and the cells were cultured to be 100% dense and maintained for 2 more days. Preadipocytes were treated with MDI (0.5 mM, 3-isobutyl-1-methylxanthine (IBMX), 1 μM dexamethasone, 1 μg/mL insulin) in 10% fetal bovine serum (FBS) DMEM medium for 2 days. to induce differentiation, and after 2 days, the medium was replaced with 10% FBS DMEM containing 1 μg/mL insulin and cultured for 2 days. After that, differentiation was induced for 8 days by replacing the culture medium with 10% FBS DMEM every 2 days. During the induction of adipocyte differentiation, each culture medium was treated with each concentration of the test substance.

Cell viability measurement (MTS assay)

After dispensing 100 μl of 1 × 10 ⁴ cell/ml 3T3-L1 progenitor cells in a 96-well culture vessel, the American kingfisher larvae extract was administered at 24 concentrations (1, 10, 50, 100 and 200 μg/ml). The reaction was carried out in an incubator for hours. After culturing for 24 hours, process the American mealworm larvae extract by concentration (the culture medium is replaced every 24 hours), add MTS reagent, and react for 3 to 4 hours to check the color change, The degree of inhibition of cell viability was confirmed by measuring the absorbance at a wavelength of 490 nm.

As shown in FIG. 1 , in all treatment groups (1, 10, 50, 100 and 200 μg/ml) treated with the American mealworm larvae extract, compared to the control group, 100% or more cell viability was exhibited. This suggests that the larvae extract according to the present invention does not show toxicity to 3T3-L1 cells and does not affect cell growth.

<Test Example 2. Anti-obesity activity assay>

Oil Red O staining method

Preadipocytes play an important role in maintaining energy homeostasis and lipid metabolism, and 3T3-L1, which are preadipocytes, are differentiated into adipocytes by various hormones and transcription factors, creating and accumulating intracellular fat.

To investigate the effect of test substances on lipid accumulation in 3T3-L1 preadipocytes, the ability to inhibit adipogenesis by Oil Red O staining was confirmed. 3T3-L1 preadipocytes were aliquoted in a 6-well plate at 1×10 ⁵ cells/well, differentiated with MDI, and then the American Mecca larvae extract was administered at each concentration (10, 50, 100 and 200 μg/ml). processed.

Intracellular lipid accumulation was visualized by Oil Red O staining on day 8. Cells were washed with phosphate buffer saline (PBS), fixed with 10% formalin, washed with distilled water, stained with Oil Red O solution (60% isopropanol aqueous solution), and the plate was washed with distilled water 3 times and dried. . The stained lipid droplets were observed under an optical microscope.

As shown in FIG. 2 , intracellular fat biosynthesis by the American mealworm larva extract was not confirmed in the cells of the MDI-untreated group that were not differentiated into adipocytes, but in the cell group in which adipocyte differentiation was induced by MDI, intracellular adipocytes Accumulation by formation and biosynthesis was observed. On the other hand, intracellular adipocytes and fat biosynthesis induced into adipocytes by MDI were In all treatment groups (10, 50, 100 and 200 μg/ml) of the extract, it was significantly inhibited in a concentration-dependent manner, and it was confirmed that the formation of lipid droplets formed in the early stage of differentiation was inhibited.

western blot test

Adipocyte differentiation is an essential process in intracellular adipocyte formation and lipid biosynthesis, and includes confluence, hormone induction, clonal expansion, growth arrest, and terminal differentiation. ) proceeds step by step. The differentiation process of adipocytes is caused by transcription factors such as CCATT/amplifier binding protein-α (C/EBPa), peroxisome proliferator-activating receptor-γ (PPARγ) and sterol regulator binding protein (SREBP-1). is regulated In particular, PPARγ and C/EBPa are involved in the formation of adipose tissue by differentiation of adipocytes, and SREBP-1 is known to play a decisive role in the regulation of body fat mass by regulating adipocyte differentiation and adipogenesis as well as adipocyte differentiation.

Accordingly, it was confirmed by Western blot test whether the expression of adipocyte differentiation-related proteins was inhibited by the treatment of the American mealworm larvae extract. After adipocyte differentiation was completed, 3T3-L1 cells were washed twice with phosphate buffered saline (PBS), followed by a protease inhibitor cocktail solution (50 μM PMSF, 10 μM Pepstatin A, 20 μM Leupeptin, RIPA lysis buffer (25 mM Tris-Hcl, pH 7.6, 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate with 100 μM Benzamidine, 50 μM Bestatin) ), 0.1% sodium dodecyl sulfate (SDS) dissolved on ice for 30 minutes, and then centrifuged for 30 minutes at 13,000 rpm at 4° C. The supernatant obtained by centrifugation was obtained by using a BCA Protein Assay kit. After quantifying the protein concentration, electrophoresis on 10-12% SDS-PAGE gel was performed, and the obtained protein was transferred to a polyvinylidene difluoride (PVDF) membrane. The membrane was blocked with 5% skim milk, , Anti-C/EBPa, anti-PPARγ, anti-SREBP1 and anti-β actin antibodies were used as primary antibodies, and HRP-conjugated anti-rabbit was used as secondary antibodies After washing 3 times with TBST for 10 minutes The expression level of the protein was analyzed by reacting with the ECL detection kit.

As shown in FIG. 3 below, the larvae extract of American kingfisher according to the present invention inhibits the expression of C/EBPa, PPARγ and SREBP-1, which are proteins related to the promotion of fat biosynthesis and adipocyte differentiation, thereby improving the biosynthesis of fat and lipid droplet. has been shown to inhibit the formation of

Through the above results, it was confirmed that the American mealworm larva extract according to the present invention has an excellent effect in the prevention, improvement and treatment of obesity by inhibiting fat biosynthesis and adipocyte differentiation.

Claims (14)

American Mealworm ( Zophobas) atratus ) A pharmaceutical composition for preventing or treating obesity, comprising the larvae extract as an active ingredient. According to claim 1,
_{The American mealworm larva extract is water, C 1-4 alcohol} , or a mixed solution thereof as a solvent to extract the American mealworm larvae, a pharmaceutical composition for preventing or treating obesity. According to claim 1,
The American king mealworm larva extract is a pharmaceutical composition for preventing or treating obesity, which is extracted using 60 to 80% by volume ethanol aqueous solution as a solvent. According to claim 1,
The American king mealworm larva extract is a pharmaceutical composition for preventing or treating obesity, which is extracted using 70% by volume ethanol aqueous solution as a solvent. According to claim 1,
The American king mealworm larva extract inhibits the in vivo adipogenesis, a pharmaceutical composition for preventing or treating obesity. According to claim 1,
The obesity is any one selected from the group consisting of visceral obesity, abdominal obesity, systemic obesity and partial obesity, obesity prevention or treatment pharmaceutical composition. American Mealworm ( Zophobas) atratus ) A food composition for preventing or improving obesity, including the larvae extract. 8. The method of claim 7,
The American king mealworm larva extract is a food composition for preventing or improving obesity, which is extracted using 60 to 80% by volume ethanol aqueous solution as a solvent. 8. The method of claim 7,
The obesity is any one selected from the group consisting of visceral obesity, abdominal obesity, systemic obesity and partial obesity, obesity prevention or food composition for improvement. American Mealworm ( Zophobas) atratus ) A feed composition for preventing or improving obesity, including the larvae extract. 11. The method of claim 10,
The American king mealworm larvae extract is a feed composition for preventing or improving obesity, which is extracted using 60 to 80% by volume ethanol aqueous solution as a solvent. 11. The method of claim 10,
The obesity is any one selected from the group consisting of visceral obesity, abdominal obesity, systemic obesity and partial obesity, obesity prevention or improvement feed composition. American Mealworm ( Zophobas) atratus ) Containing larvae extract, health functional food for preventing or improving obesity. 14. The method of claim 13,
The American mealworm larva extract is a health functional food for preventing or improving obesity, which is extracted using 60 to 80% by volume ethanol aqueous solution as a solvent.

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