KR20210057458A - Compositions for antioxidant and anti-aging comprising extracts of Chionanthus retusus - Google Patents
Compositions for antioxidant and anti-aging comprising extracts of Chionanthus retusus Download PDFInfo
- Publication number
- KR20210057458A KR20210057458A KR1020190144228A KR20190144228A KR20210057458A KR 20210057458 A KR20210057458 A KR 20210057458A KR 1020190144228 A KR1020190144228 A KR 1020190144228A KR 20190144228 A KR20190144228 A KR 20190144228A KR 20210057458 A KR20210057458 A KR 20210057458A
- Authority
- KR
- South Korea
- Prior art keywords
- extract
- activity
- antioxidant
- extracts
- flower
- Prior art date
Links
- 239000000284 extract Substances 0.000 title claims abstract description 94
- 239000000203 mixture Substances 0.000 title claims abstract description 37
- 230000003078 antioxidant effect Effects 0.000 title claims abstract description 23
- 239000003963 antioxidant agent Substances 0.000 title claims abstract description 19
- 241000576839 Chionanthus retusus Species 0.000 title abstract description 12
- 230000003712 anti-aging effect Effects 0.000 title description 5
- 239000002537 cosmetic Substances 0.000 claims abstract description 16
- 230000002087 whitening effect Effects 0.000 claims abstract description 15
- 235000013305 food Nutrition 0.000 claims abstract description 12
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 53
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 45
- 238000000034 method Methods 0.000 claims description 14
- 230000037303 wrinkles Effects 0.000 claims description 10
- 230000006872 improvement Effects 0.000 claims description 7
- 230000000694 effects Effects 0.000 abstract description 32
- 230000002401 inhibitory effect Effects 0.000 abstract description 15
- 230000002292 Radical scavenging effect Effects 0.000 abstract description 14
- 102000029816 Collagenase Human genes 0.000 abstract description 13
- 108060005980 Collagenase Proteins 0.000 abstract description 13
- OHDRQQURAXLVGJ-HLVWOLMTSA-N azane;(2e)-3-ethyl-2-[(e)-(3-ethyl-6-sulfo-1,3-benzothiazol-2-ylidene)hydrazinylidene]-1,3-benzothiazole-6-sulfonic acid Chemical compound [NH4+].[NH4+].S/1C2=CC(S([O-])(=O)=O)=CC=C2N(CC)C\1=N/N=C1/SC2=CC(S([O-])(=O)=O)=CC=C2N1CC OHDRQQURAXLVGJ-HLVWOLMTSA-N 0.000 abstract description 13
- 229960002424 collagenase Drugs 0.000 abstract description 13
- 102000003425 Tyrosinase Human genes 0.000 abstract description 9
- 108060008724 Tyrosinase Proteins 0.000 abstract description 9
- 102000016387 Pancreatic elastase Human genes 0.000 abstract description 8
- 108010067372 Pancreatic elastase Proteins 0.000 abstract description 8
- HHEAADYXPMHMCT-UHFFFAOYSA-N dpph Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1[N]N(C=1C=CC=CC=1)C1=CC=CC=C1 HHEAADYXPMHMCT-UHFFFAOYSA-N 0.000 abstract description 7
- 230000001153 anti-wrinkle effect Effects 0.000 abstract description 5
- 230000007760 free radical scavenging Effects 0.000 abstract description 2
- 230000003013 cytotoxicity Effects 0.000 abstract 1
- 231100000135 cytotoxicity Toxicity 0.000 abstract 1
- 102100020720 Calcium channel flower homolog Human genes 0.000 description 36
- 101000932468 Homo sapiens Calcium channel flower homolog Proteins 0.000 description 36
- -1 polyphenol compound Chemical class 0.000 description 19
- 238000000605 extraction Methods 0.000 description 17
- 210000003491 skin Anatomy 0.000 description 17
- 239000002904 solvent Substances 0.000 description 17
- 235000006708 antioxidants Nutrition 0.000 description 15
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 14
- 229930003935 flavonoid Natural products 0.000 description 14
- 235000017173 flavonoids Nutrition 0.000 description 14
- 238000002835 absorbance Methods 0.000 description 13
- 239000000469 ethanolic extract Substances 0.000 description 12
- 101100118981 Mus musculus Epop gene Proteins 0.000 description 11
- 150000002215 flavonoids Chemical class 0.000 description 11
- 235000013824 polyphenols Nutrition 0.000 description 11
- 238000006243 chemical reaction Methods 0.000 description 10
- 239000012153 distilled water Substances 0.000 description 9
- 230000005764 inhibitory process Effects 0.000 description 9
- 150000008442 polyphenolic compounds Chemical class 0.000 description 9
- 238000009472 formulation Methods 0.000 description 8
- 239000000243 solution Substances 0.000 description 8
- PLSAJKYPRJGMHO-UHFFFAOYSA-N ursolic acid Natural products CC1CCC2(CCC3(C)C(C=CC4C5(C)CCC(O)C(C)(C)C5CCC34C)C2C1C)C(=O)O PLSAJKYPRJGMHO-UHFFFAOYSA-N 0.000 description 8
- WCGUUGGRBIKTOS-GPOJBZKASA-N (3beta)-3-hydroxyurs-12-en-28-oic acid Chemical compound C1C[C@H](O)C(C)(C)[C@@H]2CC[C@@]3(C)[C@]4(C)CC[C@@]5(C(O)=O)CC[C@@H](C)[C@H](C)[C@H]5C4=CC[C@@H]3[C@]21C WCGUUGGRBIKTOS-GPOJBZKASA-N 0.000 description 7
- 102000019197 Superoxide Dismutase Human genes 0.000 description 7
- 108010012715 Superoxide dismutase Proteins 0.000 description 7
- 229960005070 ascorbic acid Drugs 0.000 description 7
- MGJZITXUQXWAKY-UHFFFAOYSA-N diphenyl-(2,4,6-trinitrophenyl)iminoazanium Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1N=[N+](C=1C=CC=CC=1)C1=CC=CC=C1 MGJZITXUQXWAKY-UHFFFAOYSA-N 0.000 description 7
- 229940096998 ursolic acid Drugs 0.000 description 7
- 244000020518 Carthamus tinctorius Species 0.000 description 6
- 235000003255 Carthamus tinctorius Nutrition 0.000 description 6
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 6
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 6
- REFJWTPEDVJJIY-UHFFFAOYSA-N Quercetin Chemical compound C=1C(O)=CC(O)=C(C(C=2O)=O)C=1OC=2C1=CC=C(O)C(O)=C1 REFJWTPEDVJJIY-UHFFFAOYSA-N 0.000 description 6
- 239000004480 active ingredient Substances 0.000 description 6
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 6
- WQGWDDDVZFFDIG-UHFFFAOYSA-N pyrogallol Chemical compound OC1=CC=CC(O)=C1O WQGWDDDVZFFDIG-UHFFFAOYSA-N 0.000 description 6
- 230000002000 scavenging effect Effects 0.000 description 6
- 230000007423 decrease Effects 0.000 description 5
- 235000014113 dietary fatty acids Nutrition 0.000 description 5
- 230000002849 elastaseinhibitory effect Effects 0.000 description 5
- 239000000194 fatty acid Substances 0.000 description 5
- 229930195729 fatty acid Natural products 0.000 description 5
- 239000000796 flavoring agent Substances 0.000 description 5
- 235000013355 food flavoring agent Nutrition 0.000 description 5
- 239000000843 powder Substances 0.000 description 5
- 150000003254 radicals Chemical class 0.000 description 5
- 238000010992 reflux Methods 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- 239000000758 substrate Substances 0.000 description 5
- WMBWREPUVVBILR-WIYYLYMNSA-N (-)-Epigallocatechin-3-o-gallate Chemical compound O([C@@H]1CC2=C(O)C=C(C=C2O[C@@H]1C=1C=C(O)C(O)=C(O)C=1)O)C(=O)C1=CC(O)=C(O)C(O)=C1 WMBWREPUVVBILR-WIYYLYMNSA-N 0.000 description 4
- WMBWREPUVVBILR-UHFFFAOYSA-N GCG Natural products C=1C(O)=C(O)C(O)=CC=1C1OC2=CC(O)=CC(O)=C2CC1OC(=O)C1=CC(O)=C(O)C(O)=C1 WMBWREPUVVBILR-UHFFFAOYSA-N 0.000 description 4
- 239000002211 L-ascorbic acid Substances 0.000 description 4
- 235000000069 L-ascorbic acid Nutrition 0.000 description 4
- XUMBMVFBXHLACL-UHFFFAOYSA-N Melanin Chemical compound O=C1C(=O)C(C2=CNC3=C(C(C(=O)C4=C32)=O)C)=C2C4=CNC2=C1C XUMBMVFBXHLACL-UHFFFAOYSA-N 0.000 description 4
- 235000008753 Papaver somniferum Nutrition 0.000 description 4
- 241000218180 Papaveraceae Species 0.000 description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 4
- 239000000872 buffer Substances 0.000 description 4
- 239000006071 cream Substances 0.000 description 4
- 239000006210 lotion Substances 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 239000001301 oxygen Substances 0.000 description 4
- 229910052760 oxygen Inorganic materials 0.000 description 4
- 230000009467 reduction Effects 0.000 description 4
- 238000006722 reduction reaction Methods 0.000 description 4
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 3
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 102000008186 Collagen Human genes 0.000 description 3
- 108010035532 Collagen Proteins 0.000 description 3
- 241000207834 Oleaceae Species 0.000 description 3
- ZVOLCUVKHLEPEV-UHFFFAOYSA-N Quercetagetin Natural products C1=C(O)C(O)=CC=C1C1=C(O)C(=O)C2=C(O)C(O)=C(O)C=C2O1 ZVOLCUVKHLEPEV-UHFFFAOYSA-N 0.000 description 3
- HWTZYBCRDDUBJY-UHFFFAOYSA-N Rhynchosin Natural products C1=C(O)C(O)=CC=C1C1=C(O)C(=O)C2=CC(O)=C(O)C=C2O1 HWTZYBCRDDUBJY-UHFFFAOYSA-N 0.000 description 3
- 241000245665 Taraxacum Species 0.000 description 3
- 235000005187 Taraxacum officinale ssp. officinale Nutrition 0.000 description 3
- 230000032683 aging Effects 0.000 description 3
- 235000010323 ascorbic acid Nutrition 0.000 description 3
- 239000011668 ascorbic acid Substances 0.000 description 3
- 229920001436 collagen Polymers 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 239000003602 elastase inhibitor Substances 0.000 description 3
- 239000000839 emulsion Substances 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 239000000499 gel Substances 0.000 description 3
- 239000004615 ingredient Substances 0.000 description 3
- MWDZOUNAPSSOEL-UHFFFAOYSA-N kaempferol Natural products OC1=C(C(=O)c2cc(O)cc(O)c2O1)c3ccc(O)cc3 MWDZOUNAPSSOEL-UHFFFAOYSA-N 0.000 description 3
- 239000005445 natural material Substances 0.000 description 3
- 229940079877 pyrogallol Drugs 0.000 description 3
- 229960001285 quercetin Drugs 0.000 description 3
- 235000005875 quercetin Nutrition 0.000 description 3
- 239000003642 reactive oxygen metabolite Substances 0.000 description 3
- 239000007921 spray Substances 0.000 description 3
- 238000003809 water extraction Methods 0.000 description 3
- DNIAPMSPPWPWGF-GSVOUGTGSA-N (R)-(-)-Propylene glycol Chemical compound C[C@@H](O)CO DNIAPMSPPWPWGF-GSVOUGTGSA-N 0.000 description 2
- TUSDEZXZIZRFGC-UHFFFAOYSA-N 1-O-galloyl-3,6-(R)-HHDP-beta-D-glucose Natural products OC1C(O2)COC(=O)C3=CC(O)=C(O)C(O)=C3C3=C(O)C(O)=C(O)C=C3C(=O)OC1C(O)C2OC(=O)C1=CC(O)=C(O)C(O)=C1 TUSDEZXZIZRFGC-UHFFFAOYSA-N 0.000 description 2
- 241000132536 Cirsium Species 0.000 description 2
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 2
- LCGLNKUTAGEVQW-UHFFFAOYSA-N Dimethyl ether Chemical compound COC LCGLNKUTAGEVQW-UHFFFAOYSA-N 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 206010014970 Ephelides Diseases 0.000 description 2
- 239000001263 FEMA 3042 Substances 0.000 description 2
- 229930091371 Fructose Natural products 0.000 description 2
- 239000005715 Fructose Substances 0.000 description 2
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerol Natural products OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- 240000008669 Hedera helix Species 0.000 description 2
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- 206010020772 Hypertension Diseases 0.000 description 2
- 240000002448 Ipomoea quamoclit Species 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- WTDRDQBEARUVNC-LURJTMIESA-N L-DOPA Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C(O)=C1 WTDRDQBEARUVNC-LURJTMIESA-N 0.000 description 2
- WTDRDQBEARUVNC-UHFFFAOYSA-N L-Dopa Natural products OC(=O)C(N)CC1=CC=C(O)C(O)=C1 WTDRDQBEARUVNC-UHFFFAOYSA-N 0.000 description 2
- 208000003351 Melanosis Diseases 0.000 description 2
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 2
- 240000007594 Oryza sativa Species 0.000 description 2
- 235000007164 Oryza sativa Nutrition 0.000 description 2
- LRBQNJMCXXYXIU-PPKXGCFTSA-N Penta-digallate-beta-D-glucose Natural products OC1=C(O)C(O)=CC(C(=O)OC=2C(=C(O)C=C(C=2)C(=O)OC[C@@H]2[C@H]([C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)O2)OC(=O)C=2C=C(OC(=O)C=3C=C(O)C(O)=C(O)C=3)C(O)=C(O)C=2)O)=C1 LRBQNJMCXXYXIU-PPKXGCFTSA-N 0.000 description 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
- 206010051246 Photodermatosis Diseases 0.000 description 2
- 208000012641 Pigmentation disease Diseases 0.000 description 2
- 239000002202 Polyethylene glycol Substances 0.000 description 2
- ATUOYWHBWRKTHZ-UHFFFAOYSA-N Propane Chemical compound CCC ATUOYWHBWRKTHZ-UHFFFAOYSA-N 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 2
- OUUQCZGPVNCOIJ-UHFFFAOYSA-M Superoxide Chemical compound [O-][O] OUUQCZGPVNCOIJ-UHFFFAOYSA-M 0.000 description 2
- XLOMVQKBTHCTTD-UHFFFAOYSA-N Zinc monoxide Chemical compound [Zn]=O XLOMVQKBTHCTTD-UHFFFAOYSA-N 0.000 description 2
- 230000001093 anti-cancer Effects 0.000 description 2
- 239000000440 bentonite Substances 0.000 description 2
- 229910000278 bentonite Inorganic materials 0.000 description 2
- SVPXDRXYRYOSEX-UHFFFAOYSA-N bentoquatam Chemical compound O.O=[Si]=O.O=[Al]O[Al]=O SVPXDRXYRYOSEX-UHFFFAOYSA-N 0.000 description 2
- SESFRYSPDFLNCH-UHFFFAOYSA-N benzyl benzoate Chemical compound C=1C=CC=CC=1C(=O)OCC1=CC=CC=C1 SESFRYSPDFLNCH-UHFFFAOYSA-N 0.000 description 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 2
- 150000001720 carbohydrates Chemical class 0.000 description 2
- 235000014633 carbohydrates Nutrition 0.000 description 2
- 239000001913 cellulose Substances 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 230000008030 elimination Effects 0.000 description 2
- 238000003379 elimination reaction Methods 0.000 description 2
- 229940030275 epigallocatechin gallate Drugs 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N ether Substances CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- 150000004665 fatty acids Chemical class 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 230000002503 metabolic effect Effects 0.000 description 2
- 239000000401 methanolic extract Substances 0.000 description 2
- 231100001083 no cytotoxicity Toxicity 0.000 description 2
- 239000003921 oil Substances 0.000 description 2
- 235000019198 oils Nutrition 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 230000004792 oxidative damage Effects 0.000 description 2
- 239000006072 paste Substances 0.000 description 2
- 230000008845 photoaging Effects 0.000 description 2
- 230000001766 physiological effect Effects 0.000 description 2
- 230000019612 pigmentation Effects 0.000 description 2
- 229920001223 polyethylene glycol Polymers 0.000 description 2
- SCVFZCLFOSHCOH-UHFFFAOYSA-M potassium acetate Chemical compound [K+].CC([O-])=O SCVFZCLFOSHCOH-UHFFFAOYSA-M 0.000 description 2
- USHAGKDGDHPEEY-UHFFFAOYSA-L potassium persulfate Chemical compound [K+].[K+].[O-]S(=O)(=O)OOS([O-])(=O)=O USHAGKDGDHPEEY-UHFFFAOYSA-L 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- HELXLJCILKEWJH-NCGAPWICSA-N rebaudioside A Chemical compound O([C@H]1[C@H](O)[C@@H](CO)O[C@H]([C@@H]1O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)O[C@]12C(=C)C[C@@]3(C1)CC[C@@H]1[C@@](C)(CCC[C@]1([C@@H]3CC2)C)C(=O)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O HELXLJCILKEWJH-NCGAPWICSA-N 0.000 description 2
- 235000009566 rice Nutrition 0.000 description 2
- 239000000377 silicon dioxide Substances 0.000 description 2
- 230000009759 skin aging Effects 0.000 description 2
- 230000037394 skin elasticity Effects 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 239000000600 sorbitol Substances 0.000 description 2
- 235000010356 sorbitol Nutrition 0.000 description 2
- 239000010421 standard material Substances 0.000 description 2
- 235000000346 sugar Nutrition 0.000 description 2
- 239000004094 surface-active agent Substances 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 239000000454 talc Substances 0.000 description 2
- 229910052623 talc Inorganic materials 0.000 description 2
- 229920002258 tannic acid Polymers 0.000 description 2
- LRBQNJMCXXYXIU-NRMVVENXSA-N tannic acid Chemical compound OC1=C(O)C(O)=CC(C(=O)OC=2C(=C(O)C=C(C=2)C(=O)OC[C@@H]2[C@H]([C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)O2)OC(=O)C=2C=C(OC(=O)C=3C=C(O)C(O)=C(O)C=3)C(O)=C(O)C=2)O)=C1 LRBQNJMCXXYXIU-NRMVVENXSA-N 0.000 description 2
- 229940033123 tannic acid Drugs 0.000 description 2
- 235000015523 tannic acid Nutrition 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 235000015112 vegetable and seed oil Nutrition 0.000 description 2
- 239000008158 vegetable oil Substances 0.000 description 2
- 239000001993 wax Substances 0.000 description 2
- JNYAEWCLZODPBN-JGWLITMVSA-N (2r,3r,4s)-2-[(1r)-1,2-dihydroxyethyl]oxolane-3,4-diol Chemical compound OC[C@@H](O)[C@H]1OC[C@H](O)[C@H]1O JNYAEWCLZODPBN-JGWLITMVSA-N 0.000 description 1
- BNGXYYYYKUGPPF-UHFFFAOYSA-M (3-methylphenyl)methyl-triphenylphosphanium;chloride Chemical compound [Cl-].CC1=CC=CC(C[P+](C=2C=CC=CC=2)(C=2C=CC=CC=2)C=2C=CC=CC=2)=C1 BNGXYYYYKUGPPF-UHFFFAOYSA-M 0.000 description 1
- BJEPYKJPYRNKOW-REOHCLBHSA-N (S)-malic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O BJEPYKJPYRNKOW-REOHCLBHSA-N 0.000 description 1
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
- WNWHHMBRJJOGFJ-UHFFFAOYSA-N 16-methylheptadecan-1-ol Chemical class CC(C)CCCCCCCCCCCCCCCO WNWHHMBRJJOGFJ-UHFFFAOYSA-N 0.000 description 1
- HIXDQWDOVZUNNA-UHFFFAOYSA-N 2-(3,4-dimethoxyphenyl)-5-hydroxy-7-methoxychromen-4-one Chemical compound C=1C(OC)=CC(O)=C(C(C=2)=O)C=1OC=2C1=CC=C(OC)C(OC)=C1 HIXDQWDOVZUNNA-UHFFFAOYSA-N 0.000 description 1
- HQFLTUZKIRYQSP-UHFFFAOYSA-N 3-ethyl-2h-1,3-benzothiazole-6-sulfonic acid Chemical compound OS(=O)(=O)C1=CC=C2N(CC)CSC2=C1 HQFLTUZKIRYQSP-UHFFFAOYSA-N 0.000 description 1
- 208000030507 AIDS Diseases 0.000 description 1
- 241000332371 Abutilon x hybridum Species 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
- 206010003210 Arteriosclerosis Diseases 0.000 description 1
- 108010011485 Aspartame Proteins 0.000 description 1
- 102100035882 Catalase Human genes 0.000 description 1
- 108010053835 Catalase Proteins 0.000 description 1
- 208000017667 Chronic Disease Diseases 0.000 description 1
- 241000212948 Cnidium Species 0.000 description 1
- 229920000858 Cyclodextrin Polymers 0.000 description 1
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 description 1
- 239000004375 Dextrin Substances 0.000 description 1
- 229920001353 Dextrin Polymers 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 229940122858 Elastase inhibitor Drugs 0.000 description 1
- 102000016942 Elastin Human genes 0.000 description 1
- 108010014258 Elastin Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 239000004386 Erythritol Substances 0.000 description 1
- UNXHWFMMPAWVPI-UHFFFAOYSA-N Erythritol Natural products OCC(O)C(O)CO UNXHWFMMPAWVPI-UHFFFAOYSA-N 0.000 description 1
- 239000001512 FEMA 4601 Substances 0.000 description 1
- 240000004670 Glycyrrhiza echinata Species 0.000 description 1
- 235000001453 Glycyrrhiza echinata Nutrition 0.000 description 1
- 235000006200 Glycyrrhiza glabra Nutrition 0.000 description 1
- 235000017382 Glycyrrhiza lepidota Nutrition 0.000 description 1
- 239000004378 Glycyrrhizin Substances 0.000 description 1
- 239000004166 Lanolin Substances 0.000 description 1
- 240000007472 Leucaena leucocephala Species 0.000 description 1
- 235000010643 Leucaena leucocephala Nutrition 0.000 description 1
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 108010067035 Pancrelipase Proteins 0.000 description 1
- 241000282376 Panthera tigris Species 0.000 description 1
- 206010033799 Paralysis Diseases 0.000 description 1
- 229920003171 Poly (ethylene oxide) Polymers 0.000 description 1
- 239000004952 Polyamide Substances 0.000 description 1
- 241000219000 Populus Species 0.000 description 1
- HELXLJCILKEWJH-SEAGSNCFSA-N Rebaudioside A Natural products O=C(O[C@H]1[C@@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1)[C@@]1(C)[C@@H]2[C@](C)([C@H]3[C@@]4(CC(=C)[C@@](O[C@H]5[C@H](O[C@H]6[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O6)[C@@H](O[C@H]6[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O6)[C@H](O)[C@@H](CO)O5)(C4)CC3)CC2)CCC1 HELXLJCILKEWJH-SEAGSNCFSA-N 0.000 description 1
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 1
- 208000000453 Skin Neoplasms Diseases 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 244000228451 Stevia rebaudiana Species 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- ULUAUXLGCMPNKK-UHFFFAOYSA-N Sulfobutanedioic acid Chemical compound OC(=O)CC(C(O)=O)S(O)(=O)=O ULUAUXLGCMPNKK-UHFFFAOYSA-N 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- MUCRYNWJQNHDJH-OADIDDRXSA-N Ursonic acid Chemical compound C1CC(=O)C(C)(C)[C@@H]2CC[C@@]3(C)[C@]4(C)CC[C@@]5(C(O)=O)CC[C@@H](C)[C@H](C)[C@H]5C4=CC[C@@H]3[C@]21C MUCRYNWJQNHDJH-OADIDDRXSA-N 0.000 description 1
- MUCRYNWJQNHDJH-UHFFFAOYSA-N Ursonic acid Natural products C1CC(=O)C(C)(C)C2CCC3(C)C4(C)CCC5(C(O)=O)CCC(C)C(C)C5C4=CCC3C21C MUCRYNWJQNHDJH-UHFFFAOYSA-N 0.000 description 1
- TVXBFESIOXBWNM-UHFFFAOYSA-N Xylitol Natural products OCCC(O)C(O)C(O)CCO TVXBFESIOXBWNM-UHFFFAOYSA-N 0.000 description 1
- 244000126002 Ziziphus vulgaris Species 0.000 description 1
- 235000011054 acetic acid Nutrition 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- BJEPYKJPYRNKOW-UHFFFAOYSA-N alpha-hydroxysuccinic acid Natural products OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- WNROFYMDJYEPJX-UHFFFAOYSA-K aluminium hydroxide Chemical compound [OH-].[OH-].[OH-].[Al+3] WNROFYMDJYEPJX-UHFFFAOYSA-K 0.000 description 1
- 238000000540 analysis of variance Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000010775 animal oil Substances 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 230000006851 antioxidant defense Effects 0.000 description 1
- 150000001491 aromatic compounds Chemical class 0.000 description 1
- 208000011775 arteriosclerosis disease Diseases 0.000 description 1
- 239000000605 aspartame Substances 0.000 description 1
- 235000010357 aspartame Nutrition 0.000 description 1
- IAOZJIPTCAWIRG-QWRGUYRKSA-N aspartame Chemical compound OC(=O)C[C@H](N)C(=O)N[C@H](C(=O)OC)CC1=CC=CC=C1 IAOZJIPTCAWIRG-QWRGUYRKSA-N 0.000 description 1
- 229960003438 aspartame Drugs 0.000 description 1
- 230000003796 beauty Effects 0.000 description 1
- 229960002903 benzyl benzoate Drugs 0.000 description 1
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 1
- 235000013361 beverage Nutrition 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 239000001273 butane Substances 0.000 description 1
- 239000000378 calcium silicate Substances 0.000 description 1
- 229910052918 calcium silicate Inorganic materials 0.000 description 1
- OYACROKNLOSFPA-UHFFFAOYSA-N calcium;dioxido(oxo)silane Chemical compound [Ca+2].[O-][Si]([O-])=O OYACROKNLOSFPA-UHFFFAOYSA-N 0.000 description 1
- 238000011088 calibration curve Methods 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 230000036952 cancer formation Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 125000004432 carbon atom Chemical group C* 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 150000005827 chlorofluoro hydrocarbons Chemical class 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 210000002808 connective tissue Anatomy 0.000 description 1
- 150000001893 coumarin derivatives Chemical class 0.000 description 1
- 239000012043 crude product Substances 0.000 description 1
- 238000004042 decolorization Methods 0.000 description 1
- 208000002925 dental caries Diseases 0.000 description 1
- 235000019425 dextrin Nutrition 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 150000002016 disaccharides Chemical class 0.000 description 1
- VJNCICVKUHKIIV-UHFFFAOYSA-N dopachrome Chemical compound O=C1C(=O)C=C2NC(C(=O)O)CC2=C1 VJNCICVKUHKIIV-UHFFFAOYSA-N 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 239000003995 emulsifying agent Substances 0.000 description 1
- HELXLJCILKEWJH-UHFFFAOYSA-N entered according to Sigma 01432 Natural products C1CC2C3(C)CCCC(C)(C(=O)OC4C(C(O)C(O)C(CO)O4)O)C3CCC2(C2)CC(=C)C21OC(C1OC2C(C(O)C(O)C(CO)O2)O)OC(CO)C(O)C1OC1OC(CO)C(O)C(O)C1O HELXLJCILKEWJH-UHFFFAOYSA-N 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 229940009714 erythritol Drugs 0.000 description 1
- 235000019414 erythritol Nutrition 0.000 description 1
- UNXHWFMMPAWVPI-ZXZARUISSA-N erythritol Chemical compound OC[C@H](O)[C@H](O)CO UNXHWFMMPAWVPI-ZXZARUISSA-N 0.000 description 1
- 239000000686 essence Substances 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 239000003925 fat Substances 0.000 description 1
- 235000019197 fats Nutrition 0.000 description 1
- 150000002191 fatty alcohols Chemical class 0.000 description 1
- 235000015203 fruit juice Nutrition 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 235000013376 functional food Nutrition 0.000 description 1
- 235000001727 glucose Nutrition 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- LPLVUJXQOOQHMX-UHFFFAOYSA-N glycyrrhetinic acid glycoside Natural products C1CC(C2C(C3(CCC4(C)CCC(C)(CC4C3=CC2=O)C(O)=O)C)(C)CC2)(C)C2C(C)(C)C1OC1OC(C(O)=O)C(O)C(O)C1OC1OC(C(O)=O)C(O)C(O)C1O LPLVUJXQOOQHMX-UHFFFAOYSA-N 0.000 description 1
- 229960004949 glycyrrhizic acid Drugs 0.000 description 1
- UYRUBYNTXSDKQT-UHFFFAOYSA-N glycyrrhizic acid Natural products CC1(C)C(CCC2(C)C1CCC3(C)C2C(=O)C=C4C5CC(C)(CCC5(C)CCC34C)C(=O)O)OC6OC(C(O)C(O)C6OC7OC(O)C(O)C(O)C7C(=O)O)C(=O)O UYRUBYNTXSDKQT-UHFFFAOYSA-N 0.000 description 1
- 235000019410 glycyrrhizin Nutrition 0.000 description 1
- LPLVUJXQOOQHMX-QWBHMCJMSA-N glycyrrhizinic acid Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@H](O[C@@H]1O[C@@H]1C([C@H]2[C@]([C@@H]3[C@@]([C@@]4(CC[C@@]5(C)CC[C@@](C)(C[C@H]5C4=CC3=O)C(O)=O)C)(C)CC2)(C)CC1)(C)C)C(O)=O)[C@@H]1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]1O LPLVUJXQOOQHMX-QWBHMCJMSA-N 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 208000019622 heart disease Diseases 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 235000008216 herbs Nutrition 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- MTNDZQHUAFNZQY-UHFFFAOYSA-N imidazoline Chemical class C1CN=CN1 MTNDZQHUAFNZQY-UHFFFAOYSA-N 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- SUMDYPCJJOFFON-UHFFFAOYSA-N isethionic acid Chemical compound OCCS(O)(=O)=O SUMDYPCJJOFFON-UHFFFAOYSA-N 0.000 description 1
- 229940039717 lanolin Drugs 0.000 description 1
- 235000019388 lanolin Nutrition 0.000 description 1
- 229940010454 licorice Drugs 0.000 description 1
- 229940069445 licorice extract Drugs 0.000 description 1
- 229930013686 lignan Natural products 0.000 description 1
- 235000009408 lignans Nutrition 0.000 description 1
- 150000005692 lignans Chemical class 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 239000001630 malic acid Substances 0.000 description 1
- 235000011090 malic acid Nutrition 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 230000007721 medicinal effect Effects 0.000 description 1
- 210000002752 melanocyte Anatomy 0.000 description 1
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 description 1
- 239000012046 mixed solvent Substances 0.000 description 1
- CQDGTJPVBWZJAZ-UHFFFAOYSA-N monoethyl carbonate Chemical compound CCOC(O)=O CQDGTJPVBWZJAZ-UHFFFAOYSA-N 0.000 description 1
- 150000002772 monosaccharides Chemical class 0.000 description 1
- IJDNQMDRQITEOD-UHFFFAOYSA-N n-butane Chemical compound CCCC IJDNQMDRQITEOD-UHFFFAOYSA-N 0.000 description 1
- OFBQJSOFQDEBGM-UHFFFAOYSA-N n-pentane Natural products CCCCC OFBQJSOFQDEBGM-UHFFFAOYSA-N 0.000 description 1
- 208000015122 neurodegenerative disease Diseases 0.000 description 1
- 231100000956 nontoxicity Toxicity 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 229920001542 oligosaccharide Polymers 0.000 description 1
- 150000002482 oligosaccharides Chemical class 0.000 description 1
- 238000001543 one-way ANOVA Methods 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 239000012188 paraffin wax Substances 0.000 description 1
- WVDDGKGOMKODPV-ZQBYOMGUSA-N phenyl(114C)methanol Chemical compound O[14CH2]C1=CC=CC=C1 WVDDGKGOMKODPV-ZQBYOMGUSA-N 0.000 description 1
- 235000017807 phytochemicals Nutrition 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 229930000223 plant secondary metabolite Natural products 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 229920002647 polyamide Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 229920000136 polysorbate Polymers 0.000 description 1
- 235000011056 potassium acetate Nutrition 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 239000001294 propane Substances 0.000 description 1
- 239000003380 propellant Substances 0.000 description 1
- 230000004224 protection Effects 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000012264 purified product Substances 0.000 description 1
- 235000019203 rebaudioside A Nutrition 0.000 description 1
- 238000006479 redox reaction Methods 0.000 description 1
- 239000013558 reference substance Substances 0.000 description 1
- CVHZOJJKTDOEJC-UHFFFAOYSA-N saccharin Chemical compound C1=CC=C2C(=O)NS(=O)(=O)C2=C1 CVHZOJJKTDOEJC-UHFFFAOYSA-N 0.000 description 1
- 235000019204 saccharin Nutrition 0.000 description 1
- 229940081974 saccharin Drugs 0.000 description 1
- 239000000901 saccharin and its Na,K and Ca salt Substances 0.000 description 1
- 238000007665 sagging Methods 0.000 description 1
- 229940071089 sarcosinate Drugs 0.000 description 1
- FSYKKLYZXJSNPZ-UHFFFAOYSA-N sarcosine Chemical compound C[NH2+]CC([O-])=O FSYKKLYZXJSNPZ-UHFFFAOYSA-N 0.000 description 1
- HFHDHCJBZVLPGP-UHFFFAOYSA-N schardinger α-dextrin Chemical compound O1C(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(O)C2O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC2C(O)C(O)C1OC2CO HFHDHCJBZVLPGP-UHFFFAOYSA-N 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- 201000000849 skin cancer Diseases 0.000 description 1
- 210000004927 skin cell Anatomy 0.000 description 1
- 239000000344 soap Substances 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 239000012064 sodium phosphate buffer Substances 0.000 description 1
- 229960002920 sorbitol Drugs 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 210000000434 stratum corneum Anatomy 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 150000005846 sugar alcohols Chemical class 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 239000000375 suspending agent Substances 0.000 description 1
- 229920001864 tannin Polymers 0.000 description 1
- 235000018553 tannin Nutrition 0.000 description 1
- 239000001648 tannin Substances 0.000 description 1
- 210000002435 tendon Anatomy 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 210000000515 tooth Anatomy 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 238000002137 ultrasound extraction Methods 0.000 description 1
- 238000009281 ultraviolet germicidal irradiation Methods 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 239000003021 water soluble solvent Substances 0.000 description 1
- 230000037373 wrinkle formation Effects 0.000 description 1
- 239000000811 xylitol Substances 0.000 description 1
- 229960002675 xylitol Drugs 0.000 description 1
- 235000010447 xylitol Nutrition 0.000 description 1
- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 description 1
- 239000011787 zinc oxide Substances 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
- A23V2200/30—Foods, ingredients or supplements having a functional effect on health
- A23V2200/318—Foods, ingredients or supplements having a functional effect on health having an effect on skin health and hair or coat
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/52—Stabilizers
- A61K2800/522—Antioxidants; Radical scavengers
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Mycology (AREA)
- Engineering & Computer Science (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Botany (AREA)
- Nutrition Science (AREA)
- Dermatology (AREA)
- Chemical & Material Sciences (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Cosmetics (AREA)
- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
Description
본 발명은 이팝나무(Chionanthus retusus) 추출물을 포함하는 조성물에 관한 것으로, 보다 구체적으로는 이팝나무(Chionanthus retusus) 꽃 추출물을 유효성분으로 포함하는 항산화 및 항노화용 조성물에 관한 것이다. 상기 조성물은 항산화, 주름 개선 및 피부 미백 효과가 우수하여 항산화 및 항노화를 위한 화장료 및 식품 조성물로 유용하게 사용할 수 있다.The present invention relates to a composition comprising an extract of Ipop tree ( Chionanthus retusus ), and more specifically to a composition for antioxidant and anti-aging comprising a flower extract of Ipop tree (Chionanthus retusus) as an active ingredient. The composition has excellent antioxidant, anti-wrinkle and skin whitening effects, and thus can be usefully used as a cosmetic and food composition for anti-oxidation and anti-aging.
의학의 발전과 생활수준의 향상으로 평균수명이 높아졌으며, 질병 없는 내적 건강함 이외에도 외적 아름다움과 노화에 대한 관심이 증가하고 있다. 다양한 외적 원인과 복잡한 생체 대사기전으로 인하여 서서히 진행되는 노화는 체내의 대사활성을 점진적으로 저하시키며, 각 조직과 기관의 활성 또한 떨어뜨린다. 특히 온도, 습도, 바람 등 각종 오염물질을 포함한 다양한 외부적 환경에 영향을 받는 피부는 자외선에 의한 광노화와 더불어 생물학적 산화작용으로 생성된 활성산소(reactive oxygen species, ROS)에 의해 체내 호르몬 감소와 각질층의 구조적 변화 등 피부의 탄력감소와 주름이 증가하게 되고, 기미, 주근깨 검버섯 등의 색소 침착이 일어나는 등 급격한 피부노화가 일어나며, 활성산소에 의해 각종 퇴행성 질환과 동맥경화, 심장병, 당뇨병, 고혈압, 암 등 만성질환이 가속화 된다(Halliwell & Gutteridge, 1992; Fisher et al., 1997.; Rittie & Fisher, 2002).With the advancement of medicine and the improvement of living standards, life expectancy has increased, and interest in external beauty and aging in addition to disease-free internal health is increasing. The aging that progresses gradually due to various external causes and complex metabolic mechanisms in the body gradually decreases the metabolic activity in the body, and also decreases the activity of each tissue and organ. In particular, skin that is affected by various external environments including various pollutants such as temperature, humidity, wind, etc. is reduced in body hormones and stratum corneum by photoaging caused by ultraviolet rays and reactive oxygen species (ROS) generated by biological oxidation. Skin elasticity decreases and wrinkles increase, such as structural changes in the skin, and rapid skin aging occurs, such as pigmentation such as spots and freckles, etc., and various degenerative diseases, arteriosclerosis, heart disease, diabetes, high blood pressure, cancer due to free radicals. And other chronic diseases are accelerated (Halliwell & Gutteridge, 1992; Fisher et al., 1997.; Rittie & Fisher, 2002).
최근에는 화장품과 의료의 중간 개념으로 의약품과 같은 치료효과를 기대할 수 있는 화장품을 의미하는 코스메슈티컬(cosmeceuticals)과 질병을 치료하거나 예방하는 의학적 효과를 기대할 수 있는 식품이나 음료 또는 보충제를 의미하는 뉴트리코스메틱(nutricosmetics)의 융합적인 개념을 적용하여 천연식물에서 부작용이 적고 안전한 기능성 건강소재 및 제품 개발에 대한 연구가 활발히 진행되고 있다(Surveswaran et al., 2007; Shim et al., 2014).Recently, as an intermediate concept between cosmetics and medical care, cosmeceuticals, which means cosmetics that can expect therapeutic effects such as pharmaceuticals, and nutricosmetics, which mean foods, beverages, or supplements that can expect medical effects to treat or prevent diseases. (Surveswaran et al., 2007; Shim et al., 2014).
이팝나무(Chionanthus retusus)는 물푸레나무과(Oleaceae)에 속하는 다년생 낙엽교목으로 5월-6월경 개화하는 꽃이 쌀밥과 같다 하여 이팝나무라고 부르게 되었다고 하며, 민간에서는 이팝나무 성숙 열매를 지사제와 건위제, 해열제 및 중풍 치료제로 사용하였으며, 잎은 나물로 먹거나 덖어서 차로 마시기도 하였으며(Kim, 1996; Lee 1999), 최근에는 가로수 및 조경용으로도 많이 식재되고 있다. Chionanthus retusus is a perennial deciduous tree belonging to the Oleaceae family, and it is said that the flowers blooming around May-June are the same as rice, so it is said to be called the Ipop tree. It was used as a remedy for paralysis, and the leaves were eaten or crushed as herbs and drunk as tea (Kim, 1996; Lee 1999), and recently, many plants have been planted for street trees and landscaping.
이팝나무 잎과 줄기에서는 리그난(lignan)과 쿠마린(coumarin) 화합물 등 다양한 유용성분을 함유하며(Harborne & Green, 1980; Iwagawa et al., 1985; Chien et al., 2004), 항암(Lee et al., 2004), 항산화(Kim et al., 2015) 효과가 있다고 보고된 바 있으며, 성숙 열매 과육에 대한 항산화 및 항노화 효과(Choi et al., 2017)가 있다고 알려지면서 관상용뿐만 아니라 상업적 소재로서도 관심이 증가하고 있으나 이팝나무 꽃에 대한 생리활성 및 항노화에 관한 연구는 미흡하다.The leaves and stems of the poppies contain various useful ingredients such as lignan and coumarin compounds (Harborne & Green, 1980; Iwagawa et al., 1985; Chien et al., 2004), and anticancer (Lee et al., 2004). ., 2004) and antioxidant (Kim et al., 2015), and it is known that it has antioxidant and anti-aging effects on mature fruit flesh (Choi et al., 2017). Interest is increasing, but studies on the physiological activity and anti-aging of Epop Tree flowers are insufficient.
이에 본 발명자들은 이팝나무 꽃을 코스메슈티컬과 뉴트리코스메틱 소재로서 활용 가능성을 확인하고자 상이한 용매와 온도조건에서 이팝나무 꽃을 추출하여 각 추출물에 함유된 플라보노이드와 폴리페놀 총 화합물 함량 등을 측정한 결과, 이팝나무 꽃 추출물이 천연 소재로서 충분히 활용 가능함을 발견하고, 본 발명을 완성하였다.Accordingly, the inventors of the present invention extracted the Ipop tree flowers under different solvents and temperature conditions to confirm the possibility of utilizing the Ipop tree flower as a cosmetic material and a nutricosmetic material, and measured the total flavonoid and polyphenol compound contents contained in each extract. It was found that the flower extract of Ipopaceae was sufficiently utilized as a natural material, and the present invention was completed.
본 명세서에서 인용된 문헌은 다음과 같다.The documents cited in this specification are as follows.
AOAC: Official method of analysis. 18th ed. Method 965.31. Association of official analytical chemists. Washington DC, p. 19, 2005.AOAC: Official method of analysis. 18th ed. Method 965.31. Association of official analytical chemists. Washington DC, p. 19, 2005.
Arnao MB, Cano A, Acosta M: The hydrophilic and lipophilic contribution to total antioxidant activity. Food Chem 73 : 239-244, 2001.Arnao MB, Cano A, Acosta M: The hydrophilic and lipophilic contribution to total antioxidant activity. Food Chem 73: 239-244, 2001.
Blois MS: Antioxidant determination by the use of a stable free radical. Nature 181 : 1199-1200, 1958.Blois MS: Antioxidant determination by the use of a stable free radical. Nature 181: 1199-1200, 1958.
Boligon AA, Machado MM, Athayde ML: Technical evaluation of antioxidant activity. Med Chem 4 : 517-522, 2014.Boligon AA, Machado MM, Athayde ML: Technical evaluation of antioxidant activity. Med Chem 4: 517-522, 2014.
Bravo L: Chemistry, dietary sources, metabolism, and nutritional significance. Nutr Rev 56 : 3173-3183, 1998.Bravo L: Chemistry, dietary sources, metabolism, and nutritional significance. Nutr Rev 56: 3173-3183, 1998.
Cannell RJP, Kellam SJ, Owsianka AM, Walker JM: Results of a large scale screen of microalgae for the production of protease inhibitors. Planta Med 54 : 10-14, 1988.Cannell RJP, Kellam SJ, Owsianka AM, Walker JM: Results of a large scale screen of microalgae for the production of protease inhibitors. Planta Med 54: 10-14, 1988.
Chien CT, Kuo-Huang LL, Shen YC, Zhang R, Chen SY, Yang JC, Pharis RP: Storage behavior of Chionanthus retusus seed and asynchronous development of the radicle and shoot apex during germination in relation to germination inhibitors, including abscisic acid and four phenolic glucosides. Plant Cell Physiol 45 : 1158-1167, 2004.Chien CT, Kuo-Huang LL, Shen YC, Zhang R, Chen SY, Yang JC, Pharis RP: Storage behavior of Chionanthus retusus seed and asynchronous development of the radicle and shoot apex during germination in relation to germination inhibitors, including abscisic acid and four phenolic glucosides. Plant Cell Physiol 45: 1158-1167, 2004.
Choi JH, Oh SK: Studies on the anti-aging action of Korean ginseng. J Food Sci 17 : 506-510, 1985. (Korean)Choi JH, Oh SK: Studies on the anti-aging action of Korean ginseng. J Food Sci 17: 506-510, 1985. (Korean)
Chung JH, Kang S, Varani J, Lin J, Fisher GJ, Voorhees JJ: Decreased extracellular signal-regulated kinase and increased stress-activated MAP kinase activities in aged human skin in vivo. J Invest Dermatol 115 : 117-182, 2000.Chung JH, Kang S, Varani J, Lin J, Fisher GJ, Voorhees JJ: Decreased extracellular signal-regulated kinase and increased stress-activated MAP kinase activities in aged human skin in vivo. J Invest Dermatol 115: 117-182, 2000.
DeWitt DL, Rollins TE, Day JS, Gauger JA, Smith WK: Orientation of the active site, and antigenic determinants of prostaglandin endoperoxide of synthase in the endoplasmic reticulum. J Biol Chem 256 : 10375-10382, 1981.DeWitt DL, Rollins TE, Day JS, Gauger JA, Smith WK: Orientation of the active site, and antigenic determinants of prostaglandin endoperoxide of synthase in the endoplasmic reticulum. J Biol Chem 256: 10375-10382, 1981.
Fisher GJ, Wang ZQ, Datta SC, Varani J, Kang S, Voorhees JJ: Pathophysiology of premature skin aging induced by ultraviolet light. N Engl J Med 337 : 1419-1428, 1997.Fisher GJ, Wang ZQ, Datta SC, Varani J, Kang S, Voorhees JJ: Pathophysiology of premature skin aging induced by ultraviolet light. N Engl J Med 337: 1419-1428, 1997.
Giacomoni PU, Rein G: Factors of skin ageing share common mechanism. Biogerontol 2 : 219-229, 2001.Giacomoni PU, Rein G: Factors of skin aging share common mechanism. Biogerontol 2: 219-229, 2001.
Greenstock CL: Radiation and aging: free radical damage, biological response and possible antioxidant intervention. Med Hypotheses 41 : 473-482, 1993.Greenstock CL: Radiation and aging: free radical damage, biological response and possible antioxidant intervention. Med Hypotheses 41:473-482, 1993.
Halliwell B, Gutteridge JM: Free radicals, antioxidants and human disease: Where are we now? J Lab Clinical Medicine 119 : 598-620, 1992.Halliwell B, Gutteridge JM: Free radicals, antioxidants and human disease: Where are we now? J Lab Clinical Medicine 119: 598-620, 1992.
Han DS, Kim SJ: SOD-like compounds and development of functional food. Bull Food Technology 7(4) : 41-49, 1994. (Korean)Han DS, Kim SJ: SOD-like compounds and development of functional food. Bull Food Technology 7(4): 41-49, 1994. (Korean)
Han EK, Jung EJ, Lee JY, Kin YX, Chung CK: Antioxidative activity of ethanol extracts from different parts of Taraxacum officinale. J Korean Soc Food Sci Nutr 40 : 56-62, 2011. (Korena)Han EK, Jung EJ, Lee JY, Kin YX, Chung CK: Antioxidative activity of ethanol extracts from different parts of Taraxacum officinale . J Korean Soc Food Sci Nutr 40: 56-62, 2011. (Korena)
Harborne JB, Green PS: A chemotaxonomic survey of flavonoids in leaves of the Oleaceae. Botanical J Linn Soc 81 : 155-167, 1980.Harborne JB, Green PS: A chemotaxonomic survey of flavonoids in leaves of the Oleaceae. Botanical J Linn Soc 81:155-167, 1980.
Iwagawa T, Takarabe M, Hase T: On the constituents of Chionanthus retusus. Rep Fac Sci Kagoshima Univ 18 : 49-52, 1985.Iwagawa T, Takarabe M, Hase T: On the constituents of Chionanthus retusus . Rep Fac Sci Kagoshima Univ 18: 49-52, 1985.
Jang YS, Seo SJ, Kim NW, Lee YS: Antioxidant and anti-aging Activity of Houttuyniae herba extracts obtained from different solvents. J Invest Cosmetol 15 : 147-157, 2019. (Korean) doi: 10.15810/jic.2019.15.2.004Jang YS, Seo SJ, Kim NW, Lee YS: Antioxidant and anti-aging Activity of Houttuyniae herba extracts obtained from different solvents. J Invest Cosmetol 15: 147-157, 2019. (Korean) doi: 10.15810/jic.2019.15.2.004
Kameyama K, Takemura T, Hamada Y, Sakai C, Kondoh S, Nishiyama S, Urabe K, Hearing VJ: Pigment production in murine melanoma cells is regulated by tyrosinase, tyrosinase-related protein 1(TRP 1), dopachrome tautormerase(TRP 2) and a melanogenic inhibitor. J Invest Dermatol 100 : 126-131. 1993.Kameyama K, Takemura T, Hamada Y, Sakai C, Kondoh S, Nishiyama S, Urabe K, Hearing VJ: Pigment production in murine melanoma cells is regulated by tyrosinase, tyrosinase-related protein 1 (TRP 1), dopachrome tautormerase (TRP 2 ) and a melanogenic inhibitor. J Invest Dermatol 100: 126-131. 1993.
Kim MS, Lee EH, Cho YJ: Anti-oxidative activities of extracts from Chionanthus retusus leaves, fruits and flower. Curr Res Agric Life Sci 33 : 49-56, 2015. (Korean)Kim MS, Lee EH, Cho YJ: Anti-oxidative activities of extracts from Chionanthus retusus leaves, fruits and flower. Curr Res Agric Life Sci 33: 49-56, 2015. (Korean)
Kim JH, Joo EY, Lee YS: Anti-wrinkle and anti-aging activity of Liriope platyphylla flower extract. J Invest Cosmetol 10 : 191-200. 2014. (Korean)Kim JH, Joo EY, Lee YS: Anti-wrinkle and anti-aging activity of Liriope platyphylla flower extract. J Invest Cosmetol 10: 191-200. 2014. (Korean)
Kim TJ: Korean resources plants. Seoul Univ Press Seoul, Korea, p. 255, 1996. (Korean)Kim TJ: Korean resources plants. Seoul Univ Press Seoul, Korea, p. 255, 1996. (Korean)
Kong MR, Seo SJ, Han MR, Lee YS: Anti-oxidation and anti-aging activity of three compositae species at different extraction temperatures. J Invest Cosmetol 12 : 29-37, 2016. (Korean) doi: 10.15810/jic.2016.12.1.005Kong MR, Seo SJ, Han MR, Lee YS: Anti-oxidation and anti-aging activity of three compositae species at different extraction temperatures. J Invest Cosmetol 12: 29-37, 2016. (Korean) doi: 10.15810/jic. 2016.12.1.005
Lee CH: Illustrated flora of Korea. Hyangmoonsa, Seoul, Korea, p. 616, 1999. (Korean)Lee CH: Illustrated flora of Korea. Hyangmoonsa, Seoul, Korea, p. 616, 1999. (Korean)
Lee YN, Jeong CH, Shim KH: Isolation of antioxidant and antibrowning substance from Chionanthus retusus leaves. J Kor Soc Food Sci Nutr 33 : 1419-1425, 2004.Lee YN, Jeong CH, Shim KH: Isolation of antioxidant and antibrowning substance from Chionanthus retusus leaves. J Kor Soc Food Sci Nutr 33: 1419-1425, 2004.
Marklund S, Marklund G: Involvement of superoxide amino radical in the oxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 47 : 468-474, 1975.Marklund S, Marklund G: Involvement of superoxide amino radical in the oxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 47: 468-474, 1975.
Mok JY, Kang HJ, Cho JK, Jeon IH, Kim HS, Park JM, Jeong SI, Shim JS, Jang SI: Antioxidative and anti-inflammatory effects of extracts from different organs of Cirsium japonicum var. ussuriense. Kor J Herbology 26 : 39-47, 2011. (Korean)Mok JY, Kang HJ, Cho JK, Jeon IH, Kim HS, Park JM, Jeong SI, Shim JS, Jang SI: Antioxidative and anti-inflammatory effects of extracts from different organs of Cirsium japonicum var. ussuriense . Kor J Herbology 26: 39-47, 2011. (Korean)
Nieva Moreno MI, Isla MI, Sampietro AR, Vattuone MA: Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. J Ethnopharmacol 71 : 109-114, 2000.Nieva Moreno MI, Isla MI, Sampietro AR, Vattuone MA: Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. J Ethnopharmacol 71: 109-114, 2000.
Ortonne JP: Photoprotective porperites of skin melanin. BR J Dermatal 146 : 7-10, 2002.Ortonne JP: Photoprotective porperites of skin melanin. BR J Dermatal 146: 7-10, 2002.
Pellegrin NRR, Yang M, Rice-Evans C: Screening of dietary carotenoid rich fruit extracts for antioxidant activities applying 2,2'-azinobis(2-ethylbenzothiazoline-6-sulfonic acid) radical cation de-colorization assay. Method Enzymol 299 : 379-389, 1998.Pellegrin NRR, Yang M, Rice-Evans C: Screening of dietary carotenoid rich fruit extracts for antioxidant activities applying 2,2'-azinobis(2-ethylbenzothiazoline-6-sulfonic acid) radical cation de-colorization assay. Method Enzymol 299: 379-389, 1998.
Perlish JS, Lemlich G, Fleischmajer R: Identification of collagen fibrils in scleroderma skin. J Invest Dermatol 90 : 48-54, 1998.Perlish JS, Lemlich G, Fleischmajer R: Identification of collagen fibrils in scleroderma skin. J Invest Dermatol 90: 48-54, 1998.
Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C: Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 26 : 1231-1237, 1999.Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C: Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 26:1231-1237, 1999.
Rittie L, Fisher GJ: UV-light-induced signal cascades and skin aging. Ageing Res Rev 1 : 705-720, 2002.Rittie L, Fisher GJ: UV-light-induced signal cascades and skin aging. Aging Res Rev 1: 705-720, 2002.
Seo SJ, Lee YS, Kim NW: Analysis of antioxidative effects of fruit extracts of Nandina domestica for the development of beauty materials. J Invest Cosmetol 9 : 7-14, 2013. (Korean) Seo SJ, Lee YS, Kim NW: Analysis of antioxidative effects of fruit extracts of Nandina domestica for the development of beauty materials. J Invest Cosmetol 9: 7-14, 2013. (Korean)
Shim SM, Seo SJ, Kim NW: Effects of whitening and anti-wrinkling and antioxidant activities of flower and stem extracts of Tagetes minuta. J Invest Cosmetol 10 : 181-189, 2014. (Korean)Shim SM, Seo SJ, Kim NW: Effects of whitening and anti-wrinkling and antioxidant activities of flower and stem extracts of Tagetes minuta . J Invest Cosmetol 10: 181-189, 2014. (Korean)
Shin YJ, Hwang JM, Lee SC: Antioxidant and xanthine oxidase inhibitory activities of hot water extracts of medicinal herbs. J Korean Soc Food Sci Nutr 42 : 1712-1716, 2013. (Korean)Shin YJ, Hwang JM, Lee SC: Antioxidant and xanthine oxidase inhibitory activities of hot water extracts of medicinal herbs. J Korean Soc Food Sci Nutr 42: 1712-1716, 2013. (Korean)
Surveswaran S, Cai YZ, Corke H, Sun M: Systematic evaluation of natural phenolic antioxidants from 133 Indian medicinal plants. Food Chem 102 : 938-953, 2007.Surveswaran S, Cai YZ, Corke H, Sun M: Systematic evaluation of natural phenolic antioxidants from 133 Indian medicinal plants. Food Chem 102: 938-953, 2007.
Talwar HS, Griffiths CEM, Fisher GJ, Hamilton TA, Voorhees JJ: Reduced type I and type III procollagens in photodamaged adult human skin. J Invest Dermatol 105 : 285-290, 1995.Talwar HS, Griffiths CEM, Fisher GJ, Hamilton TA, Voorhees JJ: Reduced type I and type III procollagens in photodamaged adult human skin. J Invest Dermatol 105: 285-290, 1995.
Tsuji N, Moriwaki S, Suzuki Y, Takema Y, Imokawa G: The role of elastase secreted by fibroblasts in wrinkle formation: implication through selective inhibition of elastase activity. Photochem Photobiol 74 : 283-290, 2001.Tsuji N, Moriwaki S, Suzuki Y, Takema Y, Imokawa G: The role of elastase secreted by fibroblasts in wrinkle formation: implication through selective inhibition of elastase activity. Photochem Photobiol 74: 283-290, 2001.
Wㆌnsch E, Heindrich HG: Zur quantitativen bestimmung der kollagenase. Hoppe-seyler's Zeitschrift Fur Physiologische Chemie 333 : 149-151, 1963.Wnsch E, Heindrich HG: Zur quantitativen bestimmung der kollagenase. Hoppe-seyler's Zeitschrift Fur Physiologische Chemie 333: 149-151, 1963.
Yagi A, Kanbara T, Morinobu N: Inhibition of mushroom-tyrosinase by aloe extract. Planta Med 53 : 515-517, 1987.Yagi A, Kanbara T, Morinobu N: Inhibition of mushroom-tyrosinase by aloe extract. Planta Med 53: 515-517, 1987.
Yildirim A, Mavi A, Kara AA: Determination of antioxidant and antimicrobial activities of Rumex crispus L. extracts. J Agric Food Chem 49 : 4083-4089. 2001. Yildirim A, Mavi A, Kara AA: Determination of antioxidant and antimicrobial activities of Rumex crispus L. extracts. J Agric Food Chem 49: 4083-4089. 2001.
본 발명의 하나의 목적은 이팝나무 꽃 추출물을 포함하는 항산화, 피부 주름 개선, 및/또는 피부 미백용 화장료 조성물을 제공하는 것이다.One object of the present invention is to provide an antioxidant, skin wrinkle improvement, and / or a cosmetic composition for skin whitening, comprising a flower extract of Ipopotamus.
본 발명의 다른 하나의 목적은 이팝나무 꽃 추출물을 포함하는 항산화, 피부 주름 개선, 및/또는 피부 미백용 식품 조성물을 제공하는 것이다.Another object of the present invention is to provide an antioxidant, skin wrinkle improvement, and/or a food composition for skin whitening, comprising an extract of a flower of a poppies tree.
하나의 양태로서, 본 발명은 이팝나무 꽃 추출물을 포함하는 항산화, 주름 개선 및 미백용 화장료 조성물을 제공한다.In one aspect, the present invention provides a cosmetic composition for antioxidant, wrinkle improvement, and whitening comprising an extract of a flower of a poplar tree.
본 발명에 있어서, 이팝나무(Chionanthus retusus)는 이팝나무(Chionanthus retusus)는 물푸레나무과(Oleaceae)에 속하는 다년생 낙엽교목으로 5월-6월경 개화하는 꽃이 쌀밥과 같다 하여 이팝나무라고 부르게 되었다고 하며, 이명으로 육도목(六道木), 다엽수(茶葉樹)라고도 한다.In the present invention, and that is sing that chionanthus retusus (Chionanthus retusus) by the flowers blooming around May -6 rice as a perennial deciduous tall tree that belongs to the Oleaceae (Oleaceae) chionanthus retusus chionanthus retusus (Chionanthus retusus), It is also known as Yukdomok and multi-leaved tree.
본 발명에 있어서, 추출물은 생약을 적절한 침출액으로 짜내고 침출액을 증발시켜 농축한 제제를 의미하는 것으로, 이에 제한되지는 않으나, 추출 처리에 의해 얻어지는 추출액, 추출액의 희석액 또는 농축액, 추출액을 건조하여 얻어지는 건조물, 이들의 조정제물 또는 정제물일 수 있다.In the present invention, the extract refers to a formulation obtained by squeezing a herbal medicine into an appropriate leachate and evaporating the leachate, but is not limited thereto, but is not limited thereto, an extract obtained by an extraction treatment, a dilution or concentrate of the extract, and a dried product obtained by drying the extract. , It may be a crude product or a purified product thereof.
본 발명의 이팝나무 추출물은 당업계에 공지된 일반적인 추출방법, 분리 및 정제방법을 이용하여 제조할 수 있다. 상기 추출방법으로는 열탕 추출, 열수 추출, 냉침 추출, 환류 냉각 추출 또는 초음파 추출 등의 방법을 사용할 수 있으나, 이에 제한되지는 않는다.The extract of Ipopaceae of the present invention can be prepared using a general extraction method, separation and purification method known in the art. As the extraction method, a method such as hot water extraction, hot water extraction, cold needle extraction, reflux cooling extraction, or ultrasonic extraction may be used, but is not limited thereto.
본 발명의 실시양태에 따르면, 이팝나무 추출물은 이팝나무를 분쇄한 후 추출용매, 예컨대 물, 탄소수 1~3개의 무수 또는 함수 저급 알콜 및 이들의 혼합용매로 이루어진 군에서 선택된 용매를 추출용매로 사용하여 추출할 수 있으며, 상기 추출 용매의 양은 분쇄된 이납나무 건조 중량의 2 내지 40 중량배, 바람직하게는 5 내지 35 중량배, 보다 바람직하게는 10 내지 30배로 할 수 있다.According to an embodiment of the present invention, after crushing the Ipop tree, a solvent selected from the group consisting of an extraction solvent such as water, an anhydrous or aqueous lower alcohol having 1 to 3 carbon atoms, and a mixed solvent thereof is used as the extraction solvent. It can be extracted by, and the amount of the extraction solvent may be 2 to 40 times by weight, preferably 5 to 35 times by weight, more preferably 10 to 30 times the dry weight of the pulverized lead tree.
본 발명의 하나의 구체적인 실시 태양에서는, 이팝나무 추출물은 70% 에탄올 또는 증류수를 사용하여 50 내지 90℃, 바람직하게는 60 내지 80℃의 온도에서 2 내지 5시간, 바람직하게는 2 내지 4시간 동안 환류 추출한 다음, 여과지로 여과하여 이팝나무 추출물을 수득할 수 있다.In one specific embodiment of the present invention, the extract of Ipopaceae is used for 2 to 5 hours, preferably 2 to 4 hours at a temperature of 50 to 90°C, preferably 60 to 80°C using 70% ethanol or distilled water. After reflux extraction, it can be filtered through a filter paper to obtain an Epop tree extract.
본 발명의 하나의 구체적인 실시 태양에서는, 이팝나무 추출물은 증류수를 사용하여 100 내지 120℃, 바람직하게는 100 내지 110℃의 온도에서 2 내지 5시간, 바람직하게는 2 내지 4시간 동안 압력 추출한 다음, 여과지로 여과하여 이팝나무 추출물을 수득할 수 있다.In one specific embodiment of the present invention, the extract is extracted by pressure using distilled water at a temperature of 100 to 120° C., preferably 100 to 110° C. for 2 to 5 hours, preferably 2 to 4 hours, and then, It can be filtered through a filter paper to obtain an extract of Ipopaceae.
본 발명에서 이팝나무의 사용부위는 잎, 꽃, 뿌리, 종자 모두 사용가능하나, 바람직하게는 이팝나무의 꽃을 사용하는 것을 특징으로 한다.In the present invention, the part of the Ipop tree can be used as leaves, flowers, roots, and seeds, but preferably, the flower of the Ipop tree is used.
본 발명의 조성물에는 이팝나무 추출물이 조성물의 총 중량을 기준으로 하여 0.005 내지 50 중량%, 보다 바람직하게는 0.01 내지 30 중량%, 가장 바람직하게는 0.1 내지 10 중량%로 포함할 수 있다. 이때 이팝나무 추출물의 함량이 0.005 중량% 미만일 경우 본 발명의 목적효과인 항산화, 미백 및 주름 개선효과를 수득할 수 없으며, 50 중량%를 초과할 경우 함량의 증가에 따라 효과가 비례적이지 않아 비효율적일 수 있으며 제형상의 안정성이 확보되지 않는 문제점이 있다.In the composition of the present invention, the extract of Ipopaceae may be included in an amount of 0.005 to 50% by weight, more preferably 0.01 to 30% by weight, and most preferably 0.1 to 10% by weight, based on the total weight of the composition. At this time, if the content of the extract of Ipopaceae is less than 0.005% by weight, the antioxidant, whitening, and wrinkle improvement effects, which are the objective effects of the present invention, cannot be obtained, and if it exceeds 50% by weight, the effect is not proportional to the increase in the content, so it is inefficient. It may be, and there is a problem that the stability of the formulation is not secured.
본 발명의 이팝나무 추출물을 함유하는 조성물은 항산화 효과, 주름 개선 효과 및 미 백 효과를 나타내며, 천연물질로서 세포독성이 거의 없다.The composition containing the extract of Ipopaceae of the present invention exhibits an antioxidant effect, an anti-wrinkle effect, and a whitening effect, and as a natural substance, there is almost no cytotoxicity.
본 발명의 하나의 실시양태에 따르면, 이팝나무 추출물을 유효성분으로 포함하는 화장료 조성물을 제공한다.According to one embodiment of the present invention, there is provided a cosmetic composition comprising an extract of Ipopaceae trees as an active ingredient.
본 발명의 하나의 실시양태에 따른 화장료 조성물은 유효성분으로서의 이팝나무 추출물 이외에 화장품 조성물에 통상적으로 첨가되는 성분, 예컨대 항산화제, 안정화제, 가용화제, 비타민, 안료 및 향료와 같은 통상적인 보조제, 및 담체를 추가로 첨가할 수 있다.The cosmetic composition according to an embodiment of the present invention includes ingredients commonly added to the cosmetic composition, such as antioxidants, stabilizers, solubilizers, vitamins, pigments, and flavorings, in addition to the extract of Ipop tree as an active ingredient, and Additional carriers can be added.
본 발명의 화장료 조성물은 당업계에서 통상적으로 제조되는 어떠한 제형으로도 제조될 수 있으며, 예를 들어, 용액, 현탁액, 유탁액, 페이스트, 겔, 크림, 로션, 파우더, 비누, 계면활성제-함유 클린싱, 오일, 분말 파운데이션, 유탁액 파운데이션, 왁스 파운데이션 및 스프레이 등으로 제형화될 수 있으나, 이에 한정되는 것은 아니다. 보다 상세하게는, 영양 크림, 수렴 화장수, 유연 화장수, 로션, 에센스, 영양젤 또는 마사지 크림의 제형으로 제조될 수 있다.The cosmetic composition of the present invention may be prepared in any formulation conventionally prepared in the art, for example, solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing , Oil, powder foundation, emulsion foundation, wax foundation, and may be formulated as a spray, but is not limited thereto. In more detail, it may be prepared in the form of a nourishing cream, astringent lotion, a soft lotion, lotion, essence, a nourishing gel or a massage cream.
본 발명의 제형이 페이스트, 크림 또는 겔인 경우에는 담체 성분으로서 동물성유, 식물성유, 왁스, 파라핀, 전분, 트라칸트 검, 셀룰로오스 유도체, 폴리에틸렌 글리콜, 실리콘, 벤토나이트, 실리카, 탈크 또는 산화아연 등이 이용될 수 있다.When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth gum, cellulose derivatives, polyethylene glycol, silicone, bentonite, silica, talc, or zinc oxide are used as carrier components. Can be.
본 발명의 제형이 파우더 또는 스프레이인 경우에는 담체 성분으로서 토스, 탈크, 실리카, 알루미늄 히드록시드, 칼슘 실리케이트 또는 폴리아미드 파우더가 이용될 수 있고, 특히 스프레이인 경우에는 추가적으로 클로로플루오로히드로카본, 프로판/부탄 또는 디메틸 에테르와 같은 추진체를 포함할 수 있다.When the formulation of the present invention is a powder or a spray, toss, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In particular, in the case of a spray, additional chlorofluorohydrocarbon, propane / May contain propellants such as butane or dimethyl ether.
본 발명의 제형이 용액 또는 유탁액인 경우에는 담체 성분으로서 용매, 가용화제 또는 유탁화제가 이용되고, 예컨대 물, 에탄올, 이소프로판올, 에틸 카보네이트, 에틸 아세테이트, 벤질 알코올, 벤질 벤조에이트, 프로필렌글리콜, 1,3-부틸글리콜 오일, 글리세롤 지방족 에스테르, 폴리에틸렌 글리콜 또는 소르비탄의 지방산 에스테르가 있다.When the formulation of the present invention is a solution or emulsion, a solvent, a solubilizing agent or an emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan.
본 발명의 제형이 현탁액인 경우에는 담체 성분으로서 물, 에탄올 또는 프로필렌 글리콜과 같은 액상의 희석제, 에톡실화 이소스테아릴 알코올, 폴리옥시에틸렌 소르비톨 에스테르 및 폴리옥시에틸렌 소르비탄 에스테르와 같은 현탁제, 미소결정성 셀룰로오스, 알루미늄 메타히드록시드, 벤토나이트, 아가 또는 트라칸트 검등이 이용될 수 있다.When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, and crystallites Sex cellulose, aluminum metahydroxide, bentonite, agar or tracanth gum and the like can be used.
본 발명의 제형이 계면-활성제 함유 클린징인 경우에는 담체 성분으로서 지방족 알코올 설페이트, 지방족 알코올 에테르 설페이트, 설포숙신산 모노에스테르, 이세티오네이트, 이미다졸리늄 유도체, 메틸타우레이트, 사르코시네이트, 지방산 아미드 에테르 설페이트, 알킬아미도베타인, 지방족 알코올, 지방산 글리세리드, 지방산 디에탄올아미드, 식물성 유, 라놀린 유도체 또는 에톡실화 글리세롤 지방산 에스테르 등이 이용될 수 있다.When the formulation of the present invention is a surfactant containing cleansing, as a carrier component, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, fatty alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters may be used.
또한, 본 발명의 하나의 실시양태에 따르면, 이팝나무 추출물을 유효성분으로 포함하는 식품 조성물을 제공한다.In addition, according to one embodiment of the present invention, there is provided a food composition comprising an extract of Ipopotamus as an active ingredient.
본 발명의 하나의 실시양태에 따른 식품 조성물은 유효성분으로서 이팝나무 추출물뿐만 아니라 식품 제조 시에 통상적으로 첨가되는 성분, 예를 들어, 단백질, 탄수화물, 지방, 영양소, 조미제 및 향미제를 추가로 포함할 수 있다.The food composition according to one embodiment of the present invention further comprises, as an active ingredient, not only the extract of Ipopaceae trees, but also ingredients commonly added during food production, such as proteins, carbohydrates, fats, nutrients, flavoring agents, and flavoring agents. Can include.
상기 탄수화물의 예는 모노사카라이드, 예를 들어, 포도당, 과당 등; 디사카라이드, 예를 들어 말토스, 슈크로스, 올리고당 등; 및 폴리사카라이드, 예를 들어 덱스트린, 사이클로덱스트린 등과 같은 통상적인 당 및 자일리톨, 소르비톨, 에리트리톨 등의 당알콜이다. 향미제로서 천연 향미제 [타우마틴, 스테비아 추출물 (예를 들어 레바우디오시드 A, 글리시르히진 등]) 및 합성 향미제(사카린, 아스파르탐 등)를 사용할 수 있다.Examples of the carbohydrate include monosaccharides such as glucose, fructose, and the like; Disaccharides such as maltose, sucrose, oligosaccharides, and the like; And polysaccharides, for example, common sugars such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents, natural flavoring agents [taumatin, stevia extract (eg, rebaudioside A, glycyrrhizin, etc.]) and synthetic flavoring agents (saccharin, aspartame, etc.) can be used.
예컨대, 본 발명의 식품 조성물이 드링크제로 제조되는 경우에는 본 발명의 이팝나무 추출물 이외에 구연산, 액상과당, 설탕, 포도당, 초산, 사과산, 과즙, 두충 추출액, 대추 추출액, 감초 추출액 등을 추가로 포함시킬 수 있다.For example, when the food composition of the present invention is prepared as a drink, it may further include citric acid, liquid fructose, sugar, glucose, acetic acid, malic acid, fruit juice, cephalic extract, jujube extract, licorice extract, etc. I can.
한편, 본 발명의 이팝나무 추출물은 천연물질로서 인체에 무해하며, 독성 및 부작용이 거의 없으므로 장기간 사용시에도 안심하고 사용할 수 있으며, 특히 상기한 바와 같은 화장료 및 식품 조성물에 안전하게 적용할 수 있다.On the other hand, the extract of the present invention is harmless to the human body as a natural substance, and has almost no toxicity and side effects, so it can be safely used even when used for a long period of time, and can be particularly safely applied to cosmetics and food compositions as described above.
본 발명의 이팝나무 추출물을 유효성분으로 포함하는 조성물은 SOD 유사활성, ABTS 라디칼 소거 활성, 및 DPPH 자유 라디칼 소거 활성이 우수하여 항산화 효과가 있으며, 티로시나아제 저해효과를 가져 미백효과를 가질 뿐만 아니라 콜라게나제 및 엘라스타아제 활성을 현저히 저해시킴으로써 주름 개선효과를 가진다. 또한 본 발명의 이팝나무 추출물은 세포 독성 및 피부 부작용이 없어 화장료 및 식품 조성물에 안전하게 사용할 수 있다.The composition comprising the extract of Ipopaceae of the present invention as an active ingredient has excellent SOD-like activity, ABTS radical scavenging activity, and DPPH free radical scavenging activity, so that it has an antioxidant effect, and has a tyrosinase inhibitory effect to have a whitening effect. It has a wrinkle improvement effect by remarkably inhibiting collagenase and elastase activity. In addition, the extract of the present invention has no cytotoxicity and skin side effects, so it can be safely used in cosmetics and food compositions.
도 1은 본 발명의 일 실시예에 따른 이팝나무 꽃의 에탄올 추출물(FEE), 물 추출물(FWE), 및 열수 추출물(FHWE)의 항산화(SOD) 활성을 나타낸 그림이다 (AsA는 아스코르빈산임).
도 2는 본 발명의 일 실시예에 따른 이팝나무 꽃의 에탄올 추출물(FEE), 물 추출물(FWE), 및 열수 추출물(FHWE)의 티로시나제 저해 활성을 나타낸 그림이다 (AsA는 아스코르빈산임).Figure 1 is a diagram showing the antioxidant (SOD) activity of the ethanol extract (FEE), water extract (FWE), and hot water extract (FHWE) of Ipopaceae flowers according to an embodiment of the present invention (AsA is ascorbic acid ).
Figure 2 is a diagram showing the tyrosinase inhibitory activity of the ethanol extract (FEE), the water extract (FWE), and the hot water extract (FHWE) of the flower of the Japanese poppies according to an embodiment of the present invention (AsA is ascorbic acid).
이하, 본 발명의 이해를 돕기 위하여 실시예 등을 들어 상세하게 설명하기로 한다. 그러나, 본 발명에 따른 실시예들은 여러 가지 다른 형태로 변형될 수 있으며, 본 발명의 범위가 하기 실시예들에 한정되는 것으로 해석되어서는 안 된다. 본 발명의 실시예들은 당업계에서 평균적인 지식을 가진 자에게 본 발명을 보다 완전하게 설명하기 위해 제공되는 것이다.Hereinafter, examples, etc. will be described in detail to aid understanding of the present invention. However, the embodiments according to the present invention may be modified in various forms, and the scope of the present invention should not be construed as being limited to the following examples. The embodiments of the present invention are provided to more completely describe the present invention to those of ordinary skill in the art.
실시예 1. 이팝나무 꽃 추출물의 제조 및 통계처리Example 1. Preparation and Statistical Treatment of Epop Tree Flower Extract
이팝나무(C. retusus) 꽃은 2018년 5월 20일∼6월 10일에 경북 경산시 일대에서 동정 후 채집하여 잡질 및 이물질을 제거한 후 흐르는 물에 수세하여 물기를 털어내고 그늘진 곳에서 약 6시간 정도 건조시켜 물기를 제거하고 -35℃에서 냉동 보관하면서 추출을 위한 시료로 사용하였다. C. retusus flowers are collected from May 20 to June 10, 2018 in Gyeongsan-si, Gyeongsangbuk-do. It was dried to remove moisture, and stored frozen at -35°C and used as a sample for extraction.
상기 수집되어 보관된 이팝나무 꽃은 환류냉각관을 부착시킨 둥근 플라스크에 무게의 10배(w/w)에 해당하는 70% 에탄올과 증류수를 용매로 각각 60℃와 80℃의 수욕 상에서 3시간 동안 3회 반복 환류 추출하여 이들 각각을 FEE(이팝나무 꽃의 에탄올 추출물) 및 FWE(이팝나무 꽃의 물 추출물)로 명명하고 하기 실험을 진행하였다. 또한 압력추출기(DM-701, Seoul, Korea)를 이용하여 110℃의 온도에서 시료 무게의 30배에 해당하는 증류수를 용매로 3시간 동안 추출하였으며, 이를 FHWE(이팝나무 꽃의 열수 추출물)로 명명하고 하기 실험을 진행하였다.The collected and stored Epop tree flowers are in a water bath at 60° C. and 80° C. for 3 hours using 70% ethanol and distilled water equivalent to 10 times the weight (w/w) in a round flask attached with a reflux cooling tube. Repeated reflux extraction three times, each of these was named as FEE (ethanol extract of ivy tree flower) and FWE (water extract of ivy tree flower), and the following experiment was carried out. In addition, distilled water equivalent to 30 times the weight of the sample was extracted for 3 hours with a solvent using a pressure extractor (DM-701, Seoul, Korea) at a temperature of 110°C, which was named FHWE (hot water extract of a flower of a poppies). And the following experiment was carried out.
상기 각 추출물은 여과지(Advantec No 2, Tokyo Roshi Kaisha Ltd., Tokyo, Japan)로 여과하여 회전 진공증발기(rotatory vacuum evaporator; Eyela 400 series, Tokyo, Japan)로 1/50으로 감압 농축한 후 -45℃ 이하 조건으로 동결건조기(FD 5510 SPT, Ilsin, Yangju, Korea)를 이용하여 분말로 제조하였으며, 이를 일정 농도로 증류수나 80%에 희석하여 실험을 위한 시료로 사용하였다. 항산화 및 미백 활성을 위한 대조군으로는 아스코르브산(Sigma-Aldrich Co., St Louis, MO, USA)를 동일 농도로 측정하였으며, 콜라게나제(collagenase) 저해 활성의 대조군은 에피갈로카테킨 갈레이트(epigallocatechin gallate; Sigma-Aldrich Co., St Louis, MO, USA), 엘라스타제(elastase) 저해 활성은 우르솔릭산(ursolic acid; Sigma-Aldrich Co., UK)를 대조군으로 사용하였다.Each extract was filtered with filter paper (Advantec No 2, Tokyo Roshi Kaisha Ltd., Tokyo, Japan) and concentrated under reduced pressure to 1/50 with a rotary vacuum evaporator (Eyela 400 series, Tokyo, Japan), and then -45 It was prepared as a powder using a freeze dryer (FD 5510 SPT, Ilsin, Yangju, Korea) under conditions below °C, and diluted with distilled water or 80% at a certain concentration and used as a sample for the experiment. As a control for antioxidant and whitening activity, ascorbic acid (Sigma-Aldrich Co., St Louis, MO, USA) was measured at the same concentration, and the control for collagenase inhibitory activity was epigallocatechin gallate ( epigallocatechin gallate; Sigma-Aldrich Co., St Louis, MO, USA), elastase inhibitory activity was used as a control ursolic acid (ursolic acid; Sigma-Aldrich Co., UK).
한편, 3가지 이팝나무 꽃 추출물의 실험결과는 평균(mean)±표준편차(standard deviation; SD)로 나타내었으며, SPSS 25.0 for windows program(Package for Social Science, SPSS Inc., Chicago IL, USA)을 이용하여 통계 분석하였다. 결과에 대한 유의성 검증은 one-way analysis of variance(ANOVA) test를 실시한 후, Duncan's multiple range test법에 따라 p<0.05 수준에서 사후 검정하였다.On the other hand, the experimental results of the three extracts of Epop tree flower were expressed as mean ± standard deviation (SD), and SPSS 25.0 for windows program (Package for Social Science, SPSS Inc., Chicago IL, USA) was used. Statistical analysis was performed using. To verify the significance of the results, a one-way analysis of variance (ANOVA) test was performed, followed by a post-test at p<0.05 level according to Duncan's multiple range test method.
실시예 2: 추출 방법에 따른 이팝나무 꽃 추출물의 추출 수율Example 2: Extraction Yield of Ipop Tree Flower Extract According to the Extraction Method
상이한 온도와 용매로 추출된 3가지 이팝나무 꽃 추출물의 고형분 수율은 하기 표 1에 나타낸 바와 같이 60℃의 조건에서 에탄올을 용매로 환류 추출한 이팝나무 꽃 에탄올 추출물(FEE)이 22.75%로 가장 많은 고형분이 추출되었으며, 110℃의 고압에서 물을 용매로 추출한 열수 추출물(FHWE)은 12.80%, 그리고 80℃의 조건에서 물을 용매로 추출한 물 추출물(FWE)은 10.25%로 에탄올을 용매로 추출한 FEE가 물을 용매로 추출한 FWE보다 약 2.2배 높았으며, FHWE와 비교하여도 1.7배 이상 많은 고형분이 추출된 것으로 분석되었다.As shown in Table 1 below, the yield of solids of the three extracts of Esopaceae flowers extracted with different temperatures and solvents is 22.75% of Ethanol extract (FEE) from Essopsiae flowers obtained by reflux extraction of ethanol with a solvent at 60°C, as shown in Table 1 below. Was extracted, and the hot water extract (FHWE) obtained by extracting water as a solvent at a high pressure of 110°C was 12.80%, and the water extract (FWE) obtained by extracting water as a solvent at 80°C was 10.25%. It was about 2.2 times higher than that of FWE extracted with water as a solvent, and it was analyzed that more than 1.7 times more solids were extracted compared to FHWE.
실시예 3: 총 플라보노이드와 총 폴리페놀 화합물 함량Example 3: Total Flavonoids and Total Polyphenolic Compound Content
3-1. 총 플라보노이드 함량 측정3-1. Determination of total flavonoid content
플라보노이드 총 화합물은 80% 에탄올에 분말화된 이팝나무 꽃 추출물을 일정 농도로 희석하여 Nieva et al.(2000)의 변형된 방법에 따라 측정하였다. 시료 0.1 mL에 80% 에탄올 0.4 mL과 10% 질산알루미늄(aluminum nitrate) 0.1 mL, 그리고 1 M 아세트산칼륨(potassium acetate) 0.1 mL를 혼합하고 여기에 80% 에탄올 4.3 mL를 가하여 25℃에서 40분간 반응 후, UV/VIS spectrophotometer(Optizen POP, Mecasys, Daejeon, Korea)를 사용하여 415 nm에서 흡광도를 측정하였다. 퀘르세틴(Quercetin; Sigma-Aldrich Co., Darmstadt, Germany)을 표준물질로 검량선을 측정하여 이팝나무 꽃의 3가지 추출물에 함유된 총 플라보노이드 화합물 함량을 산출하였다.The total flavonoid compound was measured according to a modified method of Nieva et al. (2000) by diluting the powdered Epop tree flower extract in 80% ethanol to a certain concentration. To 0.1 mL of sample, 0.4 mL of 80% ethanol, 0.1 mL of 10% aluminum nitrate, and 0.1 mL of 1 M potassium acetate were mixed, and 4.3 mL of 80% ethanol was added thereto, followed by reaction at 25°C for 40 minutes. Then, the absorbance was measured at 415 nm using a UV/VIS spectrophotometer (Optizen POP, Mecasys, Daejeon, Korea). Quercetin (Sigma-Aldrich Co., Darmstadt, Germany) was used as a standard to measure a calibration curve to calculate the total flavonoid compound content contained in the three extracts of Ipopaceae flowers.
3-2. 총 폴리페놀 함량 측정3-2. Determination of total polyphenol content
총 폴리페놀 화합물은 분말화된 이팝나무 꽃 추출물을 증류수에 일정 농도로 희석하여 Folin-Denis법(AOAC, 2005)으로 각 추출물 0.2 mL에 증류수 1.8 mL를 혼합하고, folin-ciocalteu's phenol reagent(Junsei Chemical Co. Tokyo, Japan) 0.2 mL를 첨가하여 5분간 실온에서 반응시켰다. 여기에 2 M Na2CO3 0.4 mL를 혼합하고 증류수를 1.4 mL 첨가한 뒤 실온의 암소에서 1시간 동안 반응 후 725 nm에서 흡광도를 측정하였다. 탄닌산(Tannic acid; Sigma-Aldrich Co., Overijse, Belgium)를 표준물질로 측정한 검량선으로 이팝나무 꽃의 각 추출물에 함유된 폴리페놀 화합물 총 함량을 산출하였다.For the total polyphenolic compounds, powdered Ipopaceae flower extract was diluted in distilled water to a certain concentration, and 1.8 mL of distilled water was mixed with 0.2 mL of each extract by the Folin-Denis method (AOAC, 2005), and folin-ciocalteu's phenol reagent (Junsei Chemical). Co. Tokyo, Japan) 0.2 mL was added and reacted at room temperature for 5 minutes. 0.4 mL of 2 M Na 2 CO 3 was mixed thereto, and 1.4 mL of distilled water was added thereto, followed by reaction in the dark at room temperature for 1 hour, and the absorbance was measured at 725 nm. Tannic acid (Sigma-Aldrich Co., Overijse, Belgium) was measured as a standard to calculate the total amount of polyphenol compounds contained in each extract of the Ipopaceae flower.
3-3. 결과3-3. result
플라보노이드를 포함한 폴리페놀계 화합물은 2개 이상의 phenolic hydroxyl(-OH)기를 가진 방향족 화합물들로 식물의 모든 부위에 함유되어 있으며, 산화-환원 반응에서 기질로 작용하여 생물학적 산화 작용의 산물로 생성된 활성산소를 효과적으로 제거하는 항산화 물질이다. 면역증강, 항염증, 항암, 항균, 고혈압 억제, 항에이즈 및 충치예방 등 다양한 생리활성을 나타내며, 자외선 차단효과도 있어 의약품을 포함한 기능성 식품과 천연 화장료 소재로도 많이 사용되고 있다(Bravo, 1998; Yildirim et al., 2001; Surveswaran et al., 2007).Polyphenolic compounds, including flavonoids, are aromatic compounds with two or more phenolic hydroxyl (-OH) groups, which are contained in all parts of plants, and act as substrates in oxidation-reduction reactions and are produced as products of biological oxidation. It is an antioxidant that effectively removes oxygen. It exhibits various physiological activities such as immunity enhancement, anti-inflammatory, anti-cancer, antibacterial, high blood pressure suppression, anti-AIDS and tooth decay prevention, and is also widely used as a functional food including pharmaceuticals and natural cosmetic materials due to its UV-blocking effect (Bravo, 1998; Yildirim. et al., 2001; Surveswaran et al., 2007).
이에 퀘르세틴(quercetin)을 표준물질로 이팝나무 꽃 추출물의 총 플라보노이드 화합물의 함량을 측정한 결과, FEE 36.02 mg/g > FWE 23.14 mg/g > FHWE 19.38 mg/g을 함유하였다. 탄닌산(Tannin acid)을 표준물질로 측정한 폴리페놀 화합물 총 함량은 FHWE 56.25 mg/g > FWE 42.44 mg/g > FEE 33.41 mg/g의 순으로 함유된 것으로 분석되었다(상기 표 1 참조). 플라보노이드는 수용성보다는 유기용매로 추출시 수율이 증가한다는 Seo 등과 Jang 등의 결과와 일치하였으며, 폴리페놀은 추출온도가 높을수록 추출수율이 증가된다는 기존의 보고(Choi et al, 2017; Kong et al, 2017)와도 일치하였다.Accordingly, as a result of measuring the content of the total flavonoid compound in the flower extract of Ipopaceae using quercetin as a standard material, FEE 36.02 mg/g> FWE 23.14 mg/g> FHWE 19.38 mg/g. The total content of the polyphenol compound measured as a standard material of tannin acid was analyzed to be contained in the order of FHWE 56.25 mg/g> FWE 42.44 mg/g> FEE 33.41 mg/g (see Table 1 above). Flavonoids are consistent with the results of Seo et al. Jang that the yield of flavonoids increases when extracted with an organic solvent rather than water-soluble, and the previous report that the extraction yield of polyphenols increases as the extraction temperature increases (Choi et al, 2017; Kong et al, 2017).
퀘르세틴과 탄닌산을 기준물질로 플라보노이드와 폴리페놀 화합물 함량을 측정한 Shim 등(2014)은 청하향초 꽃의 에탄올과 물 추출물을 61.11 mg/g과 57.70 mg/g의 플라보노이드와 426.42 mg/g과 209.60 mg/g의 폴리페놀을 함유하며, 선복화, 감국, 홍화의 25℃ 물 추출물은 플라보노이드 6.62∼13.58 mg/g, 폴리페놀 27.71∼57.02 mg/g 함유하며, 80℃ 추출물은 각각 17.57∼42.70 mg/g와 71.49∼123.96 mg/g을 함유한다는 Kong 등(2016)의 보고와 비교하면, 이팝나무 꽃이 25℃ 조건의 물 추출물보다는 많은 플라보노이드와 폴리페놀을 함유하였으나 80℃ 물 추출보다는 낮았다.Shim et al. (2014), which measured the content of flavonoids and polyphenol compounds using quercetin and tannic acid as reference substances, obtained 61.11 mg/g and 57.70 mg/g of flavonoids and 426.42 mg/g and 209.60 mg of ethanol and water extracts from Cheongha herb flowers. /g of polyphenol, and 25℃ water extracts of Seonbokhwa, Gamguk, and safflower contain 6.62~13.58 mg/g of flavonoids and 27.71~57.02 mg/g of polyphenols, and 17.57~42.70 mg/g of extracts at 80℃, respectively. Compared with the report of Kong et al. (2016) that contain g and 71.49-123.96 mg/g, the flowers of Ipopaceae trees contained more flavonoids and polyphenols than the water extract at 25°C, but lower than the water extraction at 80°C.
실시예 4: 이팝나무 꽃 추출물의 항산화 활성Example 4: Antioxidant Activity of Flower Extract of Ipopii
4-1. SOD 유사활성 측정4-1. Measurement of SOD-like activity
생체내 효소적 항산화 방어체계의 하나인 SOD(superoxide dismutase)는 생물체에 생물체의 산화적 손상을 야기시킬 수 있는 환원된 활성산소종을 과산화수소(H2O2)로 전환시키는 반응을 촉매하며(Greenstock, 1993), 카탈라아제(catalase)는 SOD에 의해 생성된 H2O2를 무해한 물 분자와 산소분자로 전환시키는 작용을 한다. 이에 SOD와 같이 슈퍼옥사이드(superoxide)를 무해한 산소로 전환시킬수는 없으나 슈퍼옥사이드(superoxide)의 반응을 억제하여 생체내 산화적 손상으로부터 보호할 수 있는 저분자의 피토케미컬(phytochemical) 물질을 SOD 유사물질이라 한다(Han & Kim, 1994).SOD (superoxide dismutase), one of the enzymatic antioxidant defense systems in vivo, catalyzes a reaction that converts reduced reactive oxygen species into hydrogen peroxide (H 2 O 2) that can cause oxidative damage to organisms (Greenstock). , 1993), catalase acts to convert H 2 O 2 produced by SOD into harmless water molecules and oxygen molecules. Therefore, it is not possible to convert superoxide into harmless oxygen like SOD, but a low-molecular phytochemical substance that can protect from oxidative damage in vivo by inhibiting the reaction of superoxide is called an SOD-like substance. (Han & Kim, 1994).
이에 Marklund & Marklund(1975)의 방법에 따라 유해 환원 산소종을 과산화수소(H2O2)로 전환시키는 반응을 촉매하는 피로갈롤(pyrogallol)의 생성량을 측정하여 SOD(superoxide dismutase) 유사활성으로 나타내었다. 이팝나무 꽃의 3가지 추출물을 증류수에 일정 농도로 희석한 시료 0.2 mL에 pH 8.5로 보정한 tris-HCl buffer(50 mM tris [hydroxymethyl] amino-methane＋10mM EDTA) 2.6mL와 7.2mM pyrogallol(Sigma-Aldrich Co., St Louis, MO, USA) 0.2mL를 첨가하여 25℃에서 10분간 반응 후, 1N HCl 0.1 mL를 가하여 반응을 정지시켜 반응액 중 산화된 pyrogallol의 양을 420nm에서 흡광도를 측정하였다. 이를 시료 첨가구와 무첨가구의 흡광도의 차이를 백분율(%)로 하여 SOD 유사활성으로 나타내었다.Accordingly, according to the method of Marklund & Marklund (1975) , the amount of pyrogallol, which catalyzes the conversion of harmful reducing oxygen species to hydrogen peroxide (H 2 O 2 ), was measured and expressed as superoxide dismutase (SOD)-like activity. . Tris-HCl buffer (50 mM tris [hydroxymethyl] amino-methane+10mM EDTA) 2.6 mL and 7.2 mM pyrogallol (Sigma-Aldrich) calibrated to pH 8.5 in 0.2 mL of a sample diluted with distilled water to a certain concentration. Co., St Louis, MO, USA) 0.2 mL was added and reacted at 25° C. for 10 minutes, and then 0.1 mL of 1N HCl was added to stop the reaction, and the amount of oxidized pyrogallol in the reaction solution was measured at 420 nm. This was expressed as SOD-like activity by using the difference in absorbance of the sample-added group and the non-added group as a percentage (%).
그 결과, 대조군인 아스코르빈산(L-ascorbic acid)보다는 낮았으나, 2,000 ug/mL 농도에서 FHWE 53.27%, FWE 48.22%, FEE 34.57%로 에탄올보다는 물을 용매로 추출한 이팝나무 꽃 추출물이 좀더 우수한 SOD 유사활성을 나타내었다(도 1 참조).As a result, it was lower than that of the control group, L-ascorbic acid, but at a concentration of 2,000 ug/mL, FHWE 53.27%, FWE 48.22%, and FEE 34.57% were extracted with water as a solvent rather than ethanol. It showed SOD-like activity (see Fig. 1).
Han 등(2011)은 1,000 ug/mL에서 민들레 꽃 에탄올 추출물은 11.20%의 SOD 유사활성을 나타낸다고 하였으며, Kong 등(2016)은 2,000 ug/mL에서 물을 용매로 추출한 선복화 31.54%, 감국 22.05%, 홍화 23.55%의 활성을 나타낸다는 결과와 비교하면 이팝나무 꽃 추출물은 우수한 SOD 유사활성을 보였으며, 특히 열수 추출물인 FHWE는 감국과 홍화보다 2배 이상 우수한 활성을 나타내었다.Han et al. (2011) reported that the ethanol extract of dandelion flowers exhibited 11.20% of SOD-like activity at 1,000 ug/mL, and Kong et al. (2016) reported 31.54% of sunbokhwa extracted with water as a solvent at 2,000 ug/mL and 22.05% of Gamguk. , Compared with the results that showed the activity of 23.55% of safflower, the flower extract of Ipopaceae showed excellent SOD-like activity. In particular, FHWE, a hot water extract, showed more than two times superior activity than that of Gamguk and safflower.
4-2. ABTS 라디칼 소거 활성 4-2. ABTS radical scavenging activity
ABTS (2,2-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical 소거능은 ABTS 용액과 과황산칼륨과의 반응에 의해 생성된 ABTS 양이온이 시료의 항산화력에 의해 제거되어 라디칼 특유의 청록색이 탈색되는 것을 이용한 측정방법이다. 친수성 및 소수성 화합물의 항산화 측정이 가능하며, 734 nm의 비교적 높은 파장에서 최대의 흡광도를 나타내어 측정하고자 하는 추출물 고유의 색소에 의한 영향이 최소한으로 작용하여 DPPH보다 정확하게 항산화 활성을 측정할 수 있는 방법으로 알려져 있다(Pellegrin et al., 1998; Arnao et al., 2001; Boligon et al., 2014).ABTS (2,2-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical scavenging ability is the characteristic blue-green color of radicals as ABTS cations generated by the reaction between ABTS solution and potassium persulfate are removed by the antioxidant power of the sample. It is a measurement method using this decolorization, it is possible to measure the antioxidant of hydrophilic and hydrophobic compounds, and it shows the maximum absorbance at a relatively high wavelength of 734 nm, so that the effect of the dye inherent to the extract to be measured is minimal, so that it is more accurate than DPPH. It is known as a method for measuring antioxidant activity (Pellegrin et al., 1998; Arnao et al., 2001; Boligon et al., 2014).
이에 Re 등(1999.)의 방법을 변형하여 (ABTS) radical에 대한 소거 활성을 측정하였다. 7 mM ABTS와 2.45 mM 과황화칼륨(potassium persulfate)를 첨가하여 상온에서 12 내지 16시간 반응시켜 ABTS 양이온을 생성시킨 후, 734 nm에서 흡광도 값이 0.70±0.02가 되도록 99.9% 에탄올로 제조하였다. 이를 ABTS 용액 1 : 시료 1의 비율로 혼합하여 7분간 실온에서 반응 후 734 nm에서 측정, 시료 무첨가구에 대한 시료 첨가구의 흡광도비로 ABTS radical 소거활성(%)을 산출하였으며, 각 추출물의 ABTS radical 소거 활성을 정확하게 파악하기 위하여 흡광도를 50% 소거하는 SC50(μg/mL)을 구하였다.Accordingly, the method of Re et al. (1999.) was modified to measure the scavenging activity against (ABTS) radicals. 7 mM ABTS and 2.45 mM potassium persulfate were added and reacted at room temperature for 12 to 16 hours to generate ABTS cations, and then prepared with 99.9% ethanol so that the absorbance value was 0.70±0.02 at 734 nm. The mixture was mixed in a ratio of ABTS solution 1:
그 결과, 이팝나무 꽃 추출물 3종은 1,000 ug/mL 이상의 농도에서 92% 이상의 우수한 소거율을 나타내었다(하기 표 2 참조). 각 추출물에 대한 SC50은 FEE 37.42 ug/mL, FWE 40.92 ug/mL, 그리고 FHWE 53.98 ug/mL으로 천연 항산화제인 L-ascorbic acid(10.65 ug/mL) 보다는 낮았으나 이팝나무 꽃 에탄올 추출물이 가장 높은 소거율을 나타내었다.As a result, three kinds of Epop tree flower extracts exhibited an excellent removal rate of 92% or more at a concentration of 1,000 ug/mL or more (see Table 2 below). SC 50 for each extract was FEE 37.42 ug/mL, FWE 40.92 ug/mL, and FHWE 53.98 ug/mL, which was lower than that of L-ascorbic acid (10.65 ug/mL), which is a natural antioxidant, but the ethanol extract of Epopaceae flowers was the highest. It shows the erasure rate.
Shin 등(2013)은 135 g/4L의 비율로 추출한 후 이를 10배로 희석한 일부 상용 한약재 추출물의 ABTS radial 소거능을 측정한 결과, 금은화 93.93%, 홍화 78.76%, 민들레 76.90%, 도라지 40.57%, 천궁 33.36%, 감초 26.12%, 현호색 8.11% 등의 소거효과가 있다고 보고하였으며, Mok 등(2011)은 엉겅퀴 꽃의 에탄올과 열수 추출물이 각각 1,000 ug/mL의 농도에서 77.5%와 88.7%의 소거율을 나타낸다는 결과와 비교하면 이팝나무 꽃 추출물이 매우 우수한 ABTS radial 소거활성을 나타내었다.Shin et al. (2013) measured the ABTS radial scavenging ability of some commercial herbal medicinal extracts extracted at a ratio of 135 g/4L and diluted 10 times. Gold and silver flower 93.93%, safflower 78.76%, dandelion 76.90%, bellflower 40.57%, cnidium 33.36%, licorice 26.12%, and brown tiger color were reported to have scavenging effects such as 8.11%, and Mok et al. (2011) reported that ethanol and hot water extracts of thistle flower produced 77.5% and 88.7% elimination rates at a concentration of 1,000 ug/mL, respectively Compared with the results shown, the flower extract of Ipopaceae showed very excellent ABTS radial scavenging activity.
(μg/mL)Con.
(μg/mL)
4-3. DPPH radical 소거 활성 4-3. DPPH radical scavenging activity
보라색을 띠는 DPPH는 안정된 radical로 항산화 물질과 반응 시 노란색으로 환원되며, 이를 517 nm 파장에서 측정하는 항산화 활성 측정에 가장 많이 사용하는 방법이다(Blois et al., 2013; Choi & Oh, 1985).DPPH, which has a purple color, is a stable radical that is reduced to yellow when reacted with antioxidants, and is the most commonly used method for measuring antioxidant activity by measuring it at a wavelength of 517 nm (Blois et al., 2013; Choi & Oh, 1985). .
상이한 조건에서 추출한 이팝나무 꽃 추출물에 대한 2,2-diphenyl-1-picryl hydrazyl(DPPH) radical에 소거능력은 Blois(1958) 방법으로 측정하였다. 일정 농도로 희석한 시료 2 mL에 0.2 mM DPPH용액(dissolved in 99% ethanol) 1 mL를 첨가하여 37℃에서 30분간 반응 후 517 nm에서 흡광도를 측정하여 시료 무첨가구에 대한 시료 첨가구의 흡광도비로 이팝나무 꽃 추출물에 함유된 DPPH radical 소거 활성을 백분율(%)로 나타내었으며, 이팝나무 꽃 추출물의 DPPH radical 소거 활성을 정확하게 파악하기 위하여 흡광도를 50% 소거하는 SC50(μg/mL)을 산출하였다.The scavenging ability of 2,2-diphenyl-1-picryl hydrazyl (DPPH) radicals of the flower extracts of Ipopaceae trees extracted under different conditions was measured by the Blois (1958) method. Add 1 mL of 0.2 mM DPPH solution (dissolved in 99% ethanol) to 2 mL of the sample diluted to a certain concentration, react at 37°C for 30 minutes, and measure the absorbance at 517 nm. The DPPH radical scavenging activity contained in the tree flower extract was expressed as a percentage (%), and in order to accurately grasp the DPPH radical scavenging activity of the tree flower extract, SC 50 (μg/mL) was calculated to remove 50% of the absorbance.
그 결과로서, 이팝나무 꽃의 3가지 추출물에 대한 DPPH radical 소거율을 31.25∼2,000 ug/mL의 농도에서 측정한 결과, FEE와 FHWE는 250 ug/mL에서 90% 이상의 소거활성을 보였으며, 125 ug/mL에서도 70% 이상으로 대조군인 L-ascorbic acid(60.66%)보다 높은 DPPH radical 소거 활성을 나타내었다(상기 표 2 참조). 500 ug/mL에서는 이팝나무 꽃의 모든 추출물이 대조군보다 높은 활성을 보였으나, 1,000 ug/mL 이상의 농도에서는 대조군과 유의적 차이가 없거나 대조군이 좀 더 높은 소거율을 나타내었다. 이팝나무 꽃 추출물은 500 ug/mL 이상의 농도에서는 추출물간의 유의적 차이가 없었으며(p<0.05), 250 ug/mL 이상의 농도에서 FEE와 FHWE는 추출물의 농도 증가에 따른 차이가 없었다. 각 추출물에 대한 SC50은 L-ascorbic acid 35.27 ug/mL > FHWE 55.49 ug/mL > FEE 63.52 ug/mL > FWE 183.72 ug/mL의 순으로 분석되었다(상기 표 2 참조). As a result, the DPPH radical scavenging rate of the three extracts of Ipopaceae flowers was measured at a concentration of 31.25 to 2,000 ug/mL. As a result, FEE and FHWE showed more than 90% scavenging activity at 250 ug/mL, 125 The DPPH radical scavenging activity was higher than that of the control group L-ascorbic acid (60.66%) at 70% or more in ug/mL (see Table 2 above). At 500 ug/mL, all extracts of Ipopaceae flowers showed higher activity than the control group, but at concentrations of 1,000 ug/mL or more, there was no significant difference from the control group, or the control group showed a higher elimination rate. There was no significant difference between the extracts at concentrations of 500 ug/mL or higher (p<0.05), and there was no difference between FEE and FHWE at concentrations of 250 ug/mL or higher. SC 50 for each extract was analyzed in the order of L-ascorbic acid 35.27 ug/mL> FHWE 55.49 ug/mL> FEE 63.52 ug/mL> FWE 183.72 ug/mL (see Table 2 above).
이러한 실험결과는 청하향초 꽃의 에탄올과 물 추출물이 1,000 ug/mL에서 각각 89.94%와 93.26%이며(Shim et al., 2014), 1,000 ug/mL의 농도의 엉겅퀴 꽃의 에탄올 추출물은 80.6%이며 열수 추출물 75.3%라는 Mok 등(2011)의 결과와 비교하면 이팝나무 꽃의 DPPH radical 소거 활성이 매우 우수하였다.These experimental results show that ethanol and water extracts of Cheongha Hyangcho flower were 89.94% and 93.26% at 1,000 ug/mL, respectively (Shim et al., 2014), and ethanol extract of thistle flower at a concentration of 1,000 ug/mL was 80.6%. Compared with the results of Mok et al. (2011) of 75.3% of hot water extract, the DPPH radical scavenging activity of Epop tree flowers was very good.
실시예 5: 이팝나무 꽃 추출물의 항주름 활성Example 5: Anti-wrinkle activity of Ipopotamus flower extract
5-1. 콜라게나제(Collagenase) 저해 활성5-1. Collagenase inhibitory activity
콜라겐(Collagen)은 피부, 힘줄, 뼈, 그리고 치아의 유기물질 대부분을 형성하며, 피부의 기계적 견고성, 결합조직의 저항성과 조직력, 피부세포의 분화 유도 및 보호와 유지 작용을 한다(Perlish et al., 1998.). 이러한 콜라겐은 자연노화에 따른 세포활성 감소와 자외선 조사에 의한 광노화와 유해환경의 노출 등으로 인하여 감소되며, 이는 피부의 탄력 감소와 피부처짐 및 주름형성의 원인이 된다(Talwar et al., 1995.; Giacomoni & Rein, 2001). 그러므로 콜라겐을 분해하는 다양한 효소 중 콜라게나제(collagenase)의 활성을 감소시키는 것은 피부의 주름억제에 매우 중요하다(Chung et al., 2000.).Collagen forms most of the organic substances in skin, tendons, bones, and teeth, and acts on mechanical firmness of the skin, resistance and tissue strength of connective tissues, and induction, protection and maintenance of skin cells differentiation (Perlish et al. , 1998.). This collagen is reduced due to decrease in cellular activity due to natural aging, photoaging due to UV irradiation, exposure to harmful environments, etc., and this is the cause of skin elasticity reduction and skin sagging and wrinkle formation (Talwar et al., 1995. ; Giacomoni & Rein, 2001). Therefore, among the various enzymes that degrade collagen, reducing the activity of collagenase is very important in suppressing skin wrinkles (Chung et al., 2000.).
이팝나무 꽃 추출물의 콜라게나제 저해율은 Wㆌnsch & Heindrich(1963)의 방법을 변형하여 4 mM의 CaCl2를 0.1 M의 Tris-HCl buffer(pH 7.5)에 4-phenylazobenzyloxycarbonyl-Pro-Leu-Gly-Pro-D-Arg(0.3 mg/mL)를 녹인 기질액 0.25 mL와 이팝나무 꽃 추출시료 0.1 mL를 혼합하여 collagenase(0.2 mg/mL) 0.15 mL를 첨가하여 실온에서 20분간 반응시켰다. 여기에 6%의 시트르산(citric acid) 0.5 mL를 가하여 반응 정지 후, 에틸아세테이트(ethyl acetate) 1.5 mL를 혼합하여 30초간 정치 후 상등액만을 취해 320 nm에서 흡광도를 측정하여, 추출물 첨가구와 무첨가구의 흡광도 감소율을 백분율(%)로 하여 콜라게나제(collagenase) 저해율로 나타내었다. 그리고 각 추출물 첨가군의 흡광도를 50%를 저해하는 농도인 IC50(μg/mL)을 구하였다.The collagenase inhibition rate of the flower extract of Ipopaceae was modified by the method of WNsch & Heindrich (1963), and 4 mM CaCl 2 was added to 4-phenylazobenzyloxycarbonyl-Pro-Leu-Gly in 0.1 M Tris-HCl buffer (pH 7.5). -Pro-D-Arg (0.3 mg/mL) was mixed with 0.25 mL of the substrate solution and 0.1 mL of the extract sample of Epop tree, and 0.15 mL of collagenase (0.2 mg/mL) was added, followed by reaction at room temperature for 20 minutes. After the reaction was stopped by adding 0.5 mL of 6% citric acid, 1.5 mL of ethyl acetate was mixed and allowed to stand for 30 seconds, and then only the supernatant was taken and the absorbance was measured at 320 nm. The reduction rate was expressed as a percentage (%) of collagenase inhibition. And IC 50 (μg/mL), which is a concentration that inhibits 50% of the absorbance of each extract-added group, was obtained.
그 결과로서, 이팝나무 꽃을 62.5∼2,000 ug/mL의 농도에서 collagenase 저해율을 측정한 결과, 대조군인 EGCG(30.58∼92.78%)보다는 낮았으나 에탄올을 용매로 추출한 FEE는 13.26∼50.76%로 IC50은 1,866.20 ug/mL였으며, FWE는 9.85∼47.73%, 그리고 고온고압에서 추출한 FHWE는 20.61∼60.53%로 IC50은 766.72 ug/mL로 FEE보다 2.4배 이상 낮은 농도에서도 50%의 collagenas 저해 활성을 나타내는 것으로 분석되었다(하기 표 3 참조).As a result, as a result of measuring the collagenase inhibition rate of Epop tree flowers at a concentration of 62.5 to 2,000 ug/mL, it was lower than that of the control EGCG (30.58 to 92.78%), but the FEE extracted with ethanol as a solvent was 13.26 to 50.76% with IC 50. Was 1,866.20 ug/mL, FWE was 9.85 to 47.73%, and FHWE extracted at high temperature and high pressure was 20.61 to 60.53%, and IC 50 was 766.72 ug/mL, showing 50% of collagenas inhibitory activity even at concentrations 2.4 times lower than FEE. Was analyzed (see Table 3 below).
Kim 등(2014)은 1,000 ug/mL 농도에서 맥문동 꽃의 메탄올 추출물 41.71%, 물 추출물 31.11%를 나타낸다고 하였으며, Shim 등(2014)은 1,000 ug/mL에서 청하향초 꽃의 에탄올과 물 추출물이 각각 69%와 23%의 collagenase 저해 활성을 나타낸다고 하였다. 이와 비교하여 이팝나무 꽃은 맥문동과 청하향초의 메탄올과 에탄올 추출물 보다는 낮았으나 물을 용매로 추출한 FWE와 FHWE는 좀더 높은 collagenase 저해율을 보였다. 또한 선복화, 감국, 홍화 물 추출물이 2,000 ug/mL에서 20.77∼31.40%의 저해 효과가 있다는 결과(Kong 등, 2016)와 비교하여도 이팝나무 꽃의 FHWE가 약 2배 이상 우수한 collagenase 저해 활성을 나타내었다.Kim et al. (2014) reported that 41.71% of methanol extract and 31.11% of water extract were represented at 1,000 ug/mL concentration, and Shim et al. (2014) reported that ethanol and water extract of Cheongha Hyangcho flower were 69 at 1,000 ug/mL, respectively. % And 23% of collagenase inhibitory activities were reported. Compared to this, flowers of Ipopaceae trees were lower than methanol and ethanol extracts of Maelungmundong and Cheonghahyangcho, but FWE and FHWE extracted with water showed higher inhibitory rate of collagenase. In addition, compared with the results that the extracts of Seonbokhwa, Gakguk, and safflower water have an inhibitory effect of 20.77~31.40% at 2,000 ug/mL (Kong et al., 2016), FHWE of Ipomoea Quamoclit has more than twice the superior collagenase inhibitory activity. Indicated.
(μg/mL)Con.
(μg/mL)
5-2. 엘라스타제(Elastase) 저해 활성 5-2. Elastase inhibitory activity
피부의 탄력을 유지하는 엘라스틴(elastin) 단백질은 그물망 구조를 형성하며, 엘라스타제(elastase)에 의해 분해되어 엘라스틴(elastain)이 절단되면 피부의 탄력 감소로 주름이 생긴다(DeWitt et al., 1981.). 그러므로 엘라스타제 저해제는 피부주름 개선 효과를 나타내며, 우르소닉산(ursolic acid) 등이 엘라스타제 저해제로 이용되고 있다(Tsuji et al., 2001.).The elastin protein, which maintains the elasticity of the skin, forms a network structure, and is decomposed by elastase, and when elastain is cut, the elasticity of the skin decreases, resulting in wrinkles (DeWitt et al., 1981). .). Therefore, elastase inhibitors have an effect of improving skin wrinkles, and ursonic acid, etc., are used as elastase inhibitors (Tsuji et al., 2001.).
이에 Cannell 등(1988.)의 방법에 따라 3가지의 이팝나무 꽃 추출물 0.5 mL에 50 mM의 Tris-HCl buffer(pH 8.6)에 녹인 porcine pancrease elastase(2.5 U/mL) 0.5 mL와 50 mM의 Tris-HCl buffer에 N-succinyl-(L-Ala)3-p-nitroanilide(0.5 mg/mL)를 녹인 기질액 1 mL를 첨가하여 25℃에서 20분간 반응 후 기질로부터 생성되는 p-nitroanilide의 생성량을 UV/VIS spectrophotometer를 이용하여 405 nm에서 측정하였다. 이를 시료 첨가구와 무첨가구의 흡광도 감소율을 백분율(%)로 표시하여 이팝나무 꽃 추출물의 엘라스타제(elastase) 저해율로 나타내었으며, 엘라스타제(elastase)의 활성을 50% 저해시키는데 필요한 농도인 IC50(μg/mL)을 산출하였다.Accordingly, 0.5 mL of porcine pancrease elastase (2.5 U/mL) dissolved in 50 mM Tris-HCl buffer (pH 8.6) in 0.5 mL of three kinds of e. -
그 결과, 2,000 ug/mL에서 FEE 72.04%, FWE 66.43%, FHWE 67.71%로 대조군인 ursolic acid 65.97% 보다 높았으며, 특히 이팝나무 꽃의 에탄올 추출물인 FEE는 모든 농도에서 대조군인 ursolic acid보다 비슷하거나 높은 elastase 저해효과를 나타내었다(상기 표 3 참조). Elastase의 활성을 50% 저해하는 농도인 IC50은 FEE 641.14 ug/mL > ursolic acid 676.55 ug/mL > FHWE 756.67 ug/mL > FWE 972.75 ug/mL로 elastase 저해제로 사용되는 ursolic acid보다도 낮은 농도에서 50%의 저해율을 나타내는 것으로 분석되었다.As a result, FEE 72.04%, FWE 66.43%, and FHWE 67.71% at 2,000 ug/mL were higher than the control ursolic acid 65.97%. In particular, FEE, the ethanol extract of Epopanthus flowers, was similar to or similar to the control ursolic acid at all concentrations. It exhibited a high elastase inhibitory effect (see Table 3 above). IC 50 , a concentration that inhibits the activity of elastase by 50%, is 50 at a concentration lower than ursolic acid used as an elastase inhibitor with FEE 641.14 ug/mL> ursolic acid 676.55 ug/mL> FHWE 756.67 ug/mL> FWE 972.75 ug/mL. % Inhibition was analyzed.
2,000 ug/mL에서 선복화 감국, 홍화의 물 추출물은 23.21∼34.67%의 elastase 저해효과가 있으며(Kong 등, 2016), 청하향초 꽃은 1,000 ug/mL에서 에탄올 추출물 20.69%, 물 추출물 34.72%라는 보고(Shim 등, 2014)와 비교하면 이팝나무 꽃은 매우 우수한 엘라스타제(elastase) 저해 활성을 나타내었다.At 2,000 ug/mL, the water extract of Seonbokhwa Gamguk and Safflower has an elastase inhibitory effect of 23.21~34.67% (Kong et al., 2016). Cheongha Hyangcho flower is 20.69% ethanol extract and 34.72% water extract at 1,000 ug/mL Compared with the report (Shim et al., 2014), the flowers of the Epopaceae tree showed very good elastase inhibitory activity.
실시예 6: 이팝나무 꽃 추출물의 미백 활성Example 6: Whitening activity of Ipopaceae flower extract
사람의 피부색 결정에 가장 중요한 요인인 멜라닌은 피부 표피 기저층에 존재하는 멜라닌 세포가 자외선에 노출되면 대항하는 기작으로서 멜라닌 소체에서 생합성되며, 피부의 각질화 세포로 이동하여 갈색과 검은색을 나타낸다(Ortonne, 2002.). 외부의 자극이나 자외선 등으로부터 피부를 보호하는 긍정적 기능도 있으나, 색소침착을 일으키는 과도한 생성은 기미, 주근깨, 검버섯 등을 유발하며, 멜라닌 전구물질의 독성으로 피부 노화와 피부암 생성이 촉진되는 부정적인 기능도 있다(Kameyama et al., 2002). 그러므로 티로시나제(tyrosinase)의 활성을 효과적으로 저해할 수 있는 물질은 피부 미백을 위한 기능성 화장료 소재로 활용될 수 있다.Melanin, the most important factor in determining human skin color, is a mechanism that counteracts melanocytes present in the base layer of the skin's epidermis when exposed to ultraviolet rays. 2002.). There is also a positive function that protects the skin from external stimuli or ultraviolet rays, but excessive production that causes pigmentation causes spots, freckles, and age spots, and the toxicity of melanin precursors also promotes skin aging and skin cancer formation. Yes (Kameyama et al., 2002). Therefore, a substance that can effectively inhibit the activity of tyrosinase can be used as a functional cosmetic material for skin whitening.
이에 Yagi 등(1987)의 방법에 따라 각 추출물에 대한 티로시나제(tyrosinase) 저해율을 측정하여 미백 활성으로 나타내었다. 175 mM sodium phosphate buffer(pH 6.8) 0.5 mL에 10 mM의 3,4-dihydroxy-L-phenyl-alanine(L-DOPA)를 녹인 기질 0.2 mL와 추출 시료 0.1 mL를 혼합하고 mushroom tyrosinase(110 U/mL) 0.2 mL 가하여 25℃에서 2분간 반응시켰다. 이를 UV/VIS spectrophotometer를 이용하여 475 nm에서 생성된 DOPA chrome을 측정하여, 이팝나무 꽃 추출시료 첨가구와 무첨가구의 흡광도 감소율을 백분율(%)로 하여 미백 활성으로 나타내었다.Thus, according to the method of Yagi et al. (1987), the inhibition rate of tyrosinase for each extract was measured and expressed as whitening activity. Mix 0.2 mL of the substrate obtained by dissolving 10 mM 3,4-dihydroxy-L-phenyl-alanine (L-DOPA) in 0.5 mL of 175 mM sodium phosphate buffer (pH 6.8) and 0.1 mL of the extracted sample, and mix mushroom tyrosinase (110 U/ mL) 0.2 mL was added and reacted at 25°C for 2 minutes. This was measured by measuring the DOPA chrome generated at 475 nm using a UV/VIS spectrophotometer, and the reduction rate of the absorbance of the group with and without the extract of Epop tree flower was expressed as a whitening activity as a percentage (%).
그 결과, 2,000 ug/mL의 농도에서 대조군(94.07%)보다는 낮았으나 FHWE 28.85%, FWE 23.11% 그리고 FEE 14.31%의 순으로 나타났다. 특히 고온 고압에서 추출한 FHWE는 62.5 ug/mL에서도 18.45%로 FEE보다 높은 티로시나제(tyrosinase) 저해효과를 나타내었다(도 2 참조).As a result, at a concentration of 2,000 ug/mL, it was lower than that of the control group (94.07%), but in the order of FHWE 28.85%, FWE 23.11% and FEE 14.31%. In particular, FHWE extracted at high temperature and high pressure showed a higher tyrosinase inhibitory effect than FEE at 18.45% even at 62.5 ug/mL (see FIG. 2).
청하향초 꽃은 1,000 ug/mL에서 에탄올 추출물 15.01%, 물 추출물 5.32%의 tyrosinase 저해율을 나타낸다고 보고하였으며(Shim 등., 2014), 민들레 에탄올 추출물은 15.06%라는 보고(Han 등, 2011)와 비교하면 이팝나무 꽃을 수용성 용매로 추출한 FWE와 FHWE가 좀더 우수한 티로시나제(tyrosinase) 저해효과가 있었다.Cheongha Hyangcho flower was reported to exhibit tyrosinase inhibition rates of 15.01% of ethanol extract and 5.32% of water extract at 1,000 ug/mL (Shim et al., 2014), compared with a report of 15.06% of dandelion ethanol extract (Han et al. FWE and FHWE obtained by extracting the flowers of Ipomoea Quamoclit with a water-soluble solvent had a more excellent tyrosinase inhibitory effect.
Claims (4)
A cosmetic composition for antioxidant, skin wrinkle improvement, or skin whitening comprising an extract of Ipopaceae flowers.
상기 이팝나무 꽃 추출물은 이팝나무 꽃의 에탄올, 물, 또는 열수 추출물인 것을 특징으로 하는 화장료 조성물.
The method of claim 1,
The cosmetic composition, characterized in that the ethanol, water, or hot water extract of the Ipop tree flower extract.
A food composition for antioxidant, skin wrinkle improvement, or skin whitening comprising an extract of Ipopaceae flowers.
상기 이팝나무 꽃 추출물은 이팝나무 꽃의 에탄올, 물, 또는 열수 추출물인 것을 특징으로 하는 식품 조성물.The method of claim 1,
The food composition, characterized in that the ethanol, water, or hot water extract of the Ipop tree flower extract.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020190144228A KR20210057458A (en) | 2019-11-12 | 2019-11-12 | Compositions for antioxidant and anti-aging comprising extracts of Chionanthus retusus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020190144228A KR20210057458A (en) | 2019-11-12 | 2019-11-12 | Compositions for antioxidant and anti-aging comprising extracts of Chionanthus retusus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| KR20210057458A true KR20210057458A (en) | 2021-05-21 |
Family
ID=76157759
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020190144228A KR20210057458A (en) | 2019-11-12 | 2019-11-12 | Compositions for antioxidant and anti-aging comprising extracts of Chionanthus retusus |
Country Status (1)
| Country | Link |
|---|---|
| KR (1) | KR20210057458A (en) |
-
2019
- 2019-11-12 KR KR1020190144228A patent/KR20210057458A/en not_active Application Discontinuation
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR101936294B1 (en) | Composition comprising for skin-whitening and anti-wrinkling extract of Rumex acetosella L. or extract of Hydrangea serrata | |
| KR101743200B1 (en) | Composition for skin whitening comprising fraction of fruit extract of Sageretia thea as effective component | |
| KR101907921B1 (en) | Composition for preventing or ameliorating skin wrinkle comprising Schisandra chinensis extract as effective component | |
| KR102431885B1 (en) | Composition for skin whitening or wrinkle improvement comprising extracts of Dendropanax morbiferus | |
| KR101197531B1 (en) | A composition for skin whitening comprising extracts or fractions of Eremochloa ophiuroides as an active ingredient | |
| KR101453258B1 (en) | Health Functional Food Composition Comprising Red Ginseng Extract, Benincasa Hispida Extract, and Apios Americana Extract for Improving Skin Beauty | |
| KR100898307B1 (en) | Cosmetic composition with the antioxidant effect protecting skins aging | |
| KR102539209B1 (en) | Cosmetic composition for skin-whitening, anti-oxidant, anti-inflammatory, skin anti-irritation and moisturizing comprising the extract of Nymphaea alba, Backhousia citriodora and Malus pumila | |
| KR101966755B1 (en) | Composition for skin-lightening or antioxidant containing extract of Jeju camellia mistletoe | |
| KR102425559B1 (en) | Composition for improving skin comprising an extract of Pueraria thomsonii or a compound derived therefrom | |
| KR100445404B1 (en) | Whitening composition containing rhodiola sachalinensis a. bor extraction | |
| KR102487655B1 (en) | Composition for anti-oxidant and skin whitening comprising Codonopsis lanceolata sprout extract as an active ingredient | |
| KR102471009B1 (en) | Cosmetic composition containing Albiggia kalkora extract for skin whitening and improving wrinkles | |
| KR20210057458A (en) | Compositions for antioxidant and anti-aging comprising extracts of Chionanthus retusus | |
| KR102356144B1 (en) | Cosmetic composition and health functional food for skin whitening and anti-wrinkle | |
| KR101249034B1 (en) | Composition for skin wrinkle improvement and whitening comprising extracts of Coriandum sativum. L as an active ingredient | |
| KR101827531B1 (en) | COSMETIC COMPOSITION FOR SKIN WHITENING CONTAINING Artemisia japonica Thunb. EXTRACTS AND PREPARING METHOD OF THE SAME | |
| KR102412907B1 (en) | Composition for skin whitening and improving skin wrinkle comprising Codonopsis lanceolata bud extract as an active ingredient | |
| KR102296938B1 (en) | Antioxidant composition comprising veronica longifolia | |
| KR102444462B1 (en) | Composition having antioxidant and skin whitening effect and its use | |
| KR102651353B1 (en) | Composition for anti-oxidation and whitening comprising golden crocus extract, and use thereof | |
| KR102644047B1 (en) | A composition for preventing or improving skin wrinkles or skin thermal aging comprising rosa multiflora extracts | |
| KR102313083B1 (en) | An anti-oxidative composition comprising an extract of Hydrangea petiolaris or a fraction thereof as an active ingredient | |
| KR102525622B1 (en) | Method of Producing Adlay Oil and Cosmetic Composition for Skin Whitening Comprising Adlay Oil Produced by the Method | |
| KR102295904B1 (en) | Cosmetic composition for skin whitening comprising Astilboides tabularis flower extracts or fraction thereof as an active ingredient |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| E902 | Notification of reason for refusal | ||
| E601 | Decision to refuse application |