KR20210050034A - A composition for improving, preventing and treating of diabetes mellitus - Google Patents

Abstract

The present invention relates to a composition for preventing, treating or alleviating diabetes, including: a first mixed extract obtained by extracting a mixture consisting of Cirsium japonicum and Taraxacum platycarpum; and a second mixed extract obtained by extracting a mixture consisting of Helianthus tuberosus, Allium cepa, Maclura, aronia, Momordica charantia and Zingiber officinale, as active ingredients. The composition has excellent antidiabetic effects and is non-toxic to be consumed in the form of food, and thus, in the long term, diabetes can be alleviated, prevented or treated through diet and lifestyle of daily life.

Description

A composition for preventing, treating or improving diabetes, including thistle, dandelion, bitter gourd, pork potato, onion, cudrania, aronia and ginger {A composition for improving, preventing and treating of diabetes mellitus}

The present invention relates to a composition for preventing, treating, or improving diabetes containing a mixture consisting of thistle, dandelion, bitter gourd, pork potato, onion, cudrania, aronia and ginger as an active ingredient.

Diabetes is one of the most important adult diseases in the world. In recent years, with rapid economic growth in Korea, the prevalence rate of diabetes reaches 10%. Currently, there are more than 240 million people worldwide, and by 2025, it has reached 380 million people worldwide. It will increase, of which 60% will occur in Asia, according to the American Medical Association (JAMA) in 2009. In particular, the onset of diabetes has been extended to the middle-aged, and due to the prolonged lifespan, it has become inevitable to develop into complications.

In other words, almost all organs in the body are damaged 10 to 20 years after being diagnosed with diabetes, and diabetic retinopathy, diabetic cataract, diabetic nephropathy, and diabetic nerves. It appears as a disease (diabetic neuropathy), heart disease, cancer, osteoporosis, etc. Chronic diabetic nephropathy is the most important cause of hemodialysis treatment and end-stage renal failure, and diabetic cataracts and retinopathy lead to blindness and eventually death.

Currently, when studying insulin-dependent diabetes (type 1 diabetes), alloxan and streptozotocin are mainly used to induce diabetes in experimental animals (Lee, JW, BI Seo, JH Park, SS Roh, YH Kim, and MR Kim. Effect of Mantidis OOtheca and Mori fructus on treatment of osteoporosis in ovariectomized rats.Kor J Herbology. 2009. 24:59-71). Alloxan selectively accumulates rapidly in pancreatic beta cells, inhibiting beta-cell glucokinase and reducing insulin secretion (Lenzen, S.. Beta-cell apoptosis in type 2 diabetes: Quantitative and functional consequences. Diabetes Metab. 2008. 34:56-64.). The zebrafish (Danio rerio) is a small tropical freshwater fish of the carp family native to India. After inducing diabetes in zebrafish, an experiment has been known to confirm the recovery of diabetes by measuring the size of the pancreatic islet after administering a drug (Kim, HT, and CH Kim. Zebrafish as a tool for functional genomics. Dev Reprod. 2003. 7:69-80).

Natural products suggested to be effective in treating diabetes include Coxibustion extract (Republic of Korea Patent Registration No. 464815), aplanaic acid derivative compound isolated from the chinensis mushroom (Republic of Korea Registration Patent No. 457690), and extract of dulcis (Korean registered patent). No. 417287), polysaccharides isolated from Angelica Angelica (International Patent Publication No. 2001-60386), and bile of ruminants (US Patent No. 6451355) may be exemplified.

Korean Patent Registration No. 464815 Korean Patent Registration No. 457690 Korean Patent Registration No. 417287

It is an object of the present invention to provide a food composition capable of preventing or improving diabetes by containing a mixture consisting of thistle, dandelion, bitter gourd, pork potato, onion, cudrania, aronia and ginger as an active ingredient.

Another object of the present invention is to provide a method of preparing a food composition capable of preventing or improving the diabetes.

Another object of the present invention is to provide a pharmaceutical composition capable of preventing or treating diabetes by containing a mixture consisting of thistle, dandelion, bitter gourd, pork potato, onion, cudrania, aronia and ginger as an active ingredient.

A food composition capable of preventing or improving diabetes of the present invention for achieving the above object comprises: a first mixed extract obtained by extracting a mixture consisting of thistle and dandelion; And a second mixed extract obtained by extracting a mixture consisting of pork potato, onion, cudrania, bitter gourd, aronia, and ginger. It can be contained as an active ingredient.

The first mixed extract may be an extract extracted at 80 to 100 °C by mixing 25 to 55 parts by weight of dandelion based on 100 parts by weight of thistle.

The second mixed extract is 80 to 100 by mixing in a weight ratio of pork potato, onion, cudrania, bitter gourd, aronia and ginger 1: 0.8-1.2: 0.1-0.8: 0.1-0.8: 0.1-0.8: 0.01-0.05 It may be an extract extracted at ℃.

The thistle may be a mixture of flowers of thistle, a mixture of stems and leaves, and roots in a weight ratio of 1: 14-25: 0.1-0.9.

The composition capable of preventing, treating, or improving diabetes of the present invention for achieving the other object described above comprises the steps of: (A) obtaining a first mixed extract by mixing and extracting milk thistle and dandelion; (B) obtaining a second mixed extract by mixing and extracting pork potato, onion, Cudrania, bitter gourd, aronia and ginger; And (C) mixing the first mixed extract and the second mixed extract.

The mixture mixed in step (C) may be concentrated to a volume of 1/3 to 1/7 based on the volume of the mixture.

A pharmaceutical composition capable of preventing or treating diabetes according to the present invention for achieving the above-described another object comprises: a first mixed extract obtained by extracting a mixture consisting of thistle and dandelion; And a second mixed extract obtained by extracting a mixture consisting of pork potato, onion, cudrania, bitter gourd, aronia, and ginger. It can be contained as an active ingredient.

The composition for preventing, treating or improving diabetes of the present invention has an excellent hypoglycemic effect, so it can be effectively used for the prevention and treatment of diabetes, and because it is not toxic, it can be consumed in the form of food.

The present invention relates to a composition for preventing, treating, or improving diabetes containing a mixture consisting of thistle, dandelion, bitter gourd, pork potato, onion, cudrania, aronia and ginger as an active ingredient.

Hereinafter, the present invention will be described in detail.

The composition for preventing, treating or improving diabetes of the present invention comprises: a first mixed extract obtained by extracting a mixture consisting of thistle and dandelion; And a second extract obtained by extracting a mixture consisting of pork potato, onion, cudrania, bitter gourd, aronia, and ginger. Contains as an active ingredient.

1st mixed extract

Thistle

The thistle used in the present invention may be white-patterned thistle (Silybum marianum (L.) Gaertn.), spiny thistle (Cirsium japonicum Fisch ex DC. var. spinossimum Kitam.), or a mixture thereof, preferably white-patterned thistle. It is to use thistle alone.

The white-patterned thistle (Silybum marianum (L.) Gaertn.) is a deep, shiny green plant with milky white veins, and is a long, elongated plant divided into root-leaves with wavy patterns and thorns at the edges. It is mainly found in levee fences and in abandoned land. Useful parts of plants are whole herbs, roots, leaves, seeds, and shells, and thistle seeds contain a flavonoid compound known as silymarin. Thistle seed extract contains 70-80% silymarin.

Thistle according to the present invention is a mixed extract of a thistle flower, a mixture of stems and leaves, and a mixture of roots.

The flowers of thistle are purple or red corolla and 19-24 mm in length, and it is preferable to use only those having a length of 10 to 15 mm and not use those with a length longer than that.

In addition, the thistle stem and leaves are used by mixing in a weight ratio of 1: 1-4, preferably in a weight ratio of 1: 2-3. When the content of leaves is less than the lower limit based on the stem, the bitter taste may be strong, and when the content of the leaves is greater than the upper limit, it is not preferable for preventing, treating, or improving diabetes.

In addition, the thistle root is harvested once every three years, unlike flowers, stems, and leaves that are harvested twice a year, so it is preferable to use it after drying.

The flower of thistle, a mixture of stems and leaves, and roots are mixed in a weight ratio of 1: 14-25: 0.1-0.9, preferably 1: 16-19: 0.4-0.8. If the content of stem and leaf mixture and root based on flowers is less than the lower limit, the effect on preventing, treating, or improving diabetes may not be excellent, and if it exceeds the upper limit, the bitter taste is strong and the prevention of diabetes, The effect on treatment or improvement may be diminished.

The mixed extract of the present invention is a bath extract extracted for 20 to 30 hours, preferably 24 to 28 hours at 80 to 100 ℃. When the extraction temperature and time are less than the lower limit, the active ingredient may not be extracted, and when the extraction temperature and time exceed the upper limit, the tan taste may be strong and the nutrient component may be destroyed. In addition, when the extraction is performed by other methods such as organic solvent extraction other than the water bath extraction, the effect on preventing, treating, or improving diabetes may be insignificant and a strong bitter taste may occur.

Specifically, the mixed extract is mixed with a flower, and a mixture of stems and leaves to extract the middle bath, and the roots are separately extracted with the bath and mixed later. Unlike the present invention, in the case of extracting the water by mixing flowers, a mixture of stems and leaves, and roots, the bitter taste is strong, and the effect on preventing, treating, or improving diabetes may be lowered.

Specifically, the method of preparing the mixed thistle extract includes the steps of: (a) mixing a mixture of flowers of thistle, and a mixture of stems and leaves, and extracting the extract by boiling water at 80 to 100° C. to obtain an extract; (b) extracting the roots of thistle in a bath at 80 to 100° C. to obtain an extract; And (c) lyophilizing and powdering the extracts obtained in steps (a) and (b), respectively, and mixing them. In addition, after the step (c), (d) drying and pulverizing the residue extracted in the step (b); And (e) mixing 100 parts by weight of the powder mixed in step (c) with 0.1 to 3 parts by weight of the powder in step (d).

Unlike the above steps (a) and (b), when the bath is extracted by mixing flowers, a mixture of stems and leaves, and all of the roots, the bitter taste is strong and the effect on preventing, treating or improving diabetes may be lowered. ; When the flowers, the mixture of stems and leaves, and the roots are all extracted in a bath, the yield may decrease.

When the root residue powder prepared in step (D) is added, the effect on diseases can be significantly improved compared to the case where the root residue powder is not added.

dandelion

The dandelion (Taraxacum platycarpum) is also referred to as Geumjamcho, Jeongji, Pogongyeong, Pogongcho, Anjilbang, and also has the nickname of Satbang. Leaves or stems evenly contain proteins, carbohydrates, fats, ash, minerals, and vitamins. Young shoots are used as herbs or soup. In oriental medicine, the roots and outposts before flowering are said to be effective in antipyretic, anti-inflammatory, diuretic, and dry stomach. Use.

These dandelions are mixed in an amount of 25 to 55 parts by weight, preferably 35 to 50 parts by weight, based on 100 parts by weight of thistle.

If the content of dandelion is less than the above lower limit, the effect of preventing, treating or improving diabetes may be lower than that of using each substance alone, and if it exceeds the upper limit, the effect of preventing, treating or improving diabetes will be reduced. I can.

The first mixed extract of the present invention is extracted by mixing a mixture of thistle and dandelion and an extraction solvent in a weight ratio of 1: 1 to 100, preferably 1: 5 to 20, and extracting at 80 to 100° C. for 40 to 50 hours Prepare the extract. When the weight ratio of the mixture and the extraction solvent is out of the above range, the active ingredient of thistle and dandelion mixture may be extracted in a small amount in the extract.

The extraction solvent for extracting the extract is water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof. The extraction solvent is not particularly limited, but the extract extracted with water exhibits an excellent effect.

2nd mixed extract

The second mixed extract is a weight ratio of pork potato, onion, cudrania, bitter gourd, aronia and ginger: 1: 0.8-1.2: 0.1-0.8: 0.1-0.8: 0.1-0.8: 0.01-0.05, preferably 1: 0.9 -1: 0.2-0.5: 0.2-0.5: 0.2-0.5: 0.02-0.01 weight ratio, more preferably 1: 1: 0.24: 0.24: 0.24: 0.024 weight ratio of the mixture was extracted. In particular, it contains pork potatoes and onions in the same amount; The effect of preventing, treating or improving diabetes can be remarkably improved when Cudrania, bitter gourd, and aronia are contained in the same amount.

If the content of onion, Cudrania tree, bitter gourd, aronia, and ginger based on pork potato is less than the lower limit, the effect of preventing, treating or improving diabetes may not be improved, and if it exceeds the upper limit, the bitter taste is strong and diabetes. The preventive, therapeutic or ameliorating effect of may be lower than when each substance is used alone.

The second mixed extract is 80 by mixing a mixture of pork potato, onion, cudrania, bitter gourd, aronia, and ginger and an extraction solvent in a weight ratio of 1: 1 to 100, preferably 1: 3 to 20 It is an extract by extracting for 5 to 20 hours at to 100 ℃.

The extraction solvent for extracting the extract is water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof. The extraction solvent is not particularly limited, but the extract extracted with water exhibits an excellent effect.

The pork potato (Helianthus tuberosus) is a naturalized plant that is estimated to have been introduced to Korea after the 17th century through China from Europe, and grows wildly in fields or in wild mountains. If you dig under the dry stem, you can find tubers of pork potato, and the flowers blooming in autumn are very attractive.

In addition, the onion (onion) contains a spicy ingredient'allysin', so it has a spicy and sweet taste, has an antioxidant effect and an effect of lowering blood cholesterol levels.

In addition, the Cudrania tricuspidata Bureau is a deciduous broad-leaved small arbor belonging to the Moraceae family. There are features.

As the name of the herbal medicine, the tree trunk is called Jamok, and the leaves are used as a substitute for mulberry leaves when striking silkworms, and the fruits are used to make jam or to make liquor for food. Donguibogam has nourishing and tonic effects, and has great effects on physical weakness, energy loss, sound level, insomnia, and vision loss, and the stem bark and roots are recorded as good for female diseases. Studies on the antioxidant, anticancer, antidiabetic, and antibacterial effects of Cudrania tree have been published. In particular, various parts of Cudrania tree have been investigated to have high antioxidant properties.

In addition, the bitter gourd (Momordica charantia Linnaeus) is traditionally widely used for medicinal purposes, but there is a risk of miscarriage when a pregnant woman takes a large amount of bitter gourd. The juice obtained by squeezing leaves is applied to insect bites, stings, burns, small wounds, skin allergies, etc. to relieve itching and leave no scars. Leaf and fruit extracts are used to treat stomach pain, fever, arthritis, diabetes, high blood pressure, cancer, and infectious diseases, and are particularly effective in lowering blood sugar.

In addition, the aronia is a fruit corresponding to the genus Rosaceae Aronia, and has excellent antioxidant and anti-cancer effects due to the highest anthocyanin content among berries, and has only effects such as weight loss, liver damage prevention, inflammation relief, and eye fatigue. In addition, it lowers cholesterol and helps prevent cardiovascular disease and stroke.

In addition, the ginger, as a rootstock, grows sideways, is fleshy, has a lump shape, is yellow, has a spicy taste and a fragrant smell. Ginger cures chills, fever, headache, vomiting, seawater, and phlegm caused by colds. It is also effective for abdominal pain, diarrhea and stomach caused by food poisoning. As pharmacological action, gastric juice secretion promotion, digestion enhancement, heart excitation, blood circulation promotion, and inhibition have been reported.

In addition, the present invention provides a method of preparing a composition for preventing, treating or improving diabetes.

The method for preparing a composition for preventing, treating or improving diabetes of the present invention comprises the steps of: (A) obtaining a first mixed extract by mixing and extracting thistle and dandelion; (B) obtaining a second mixed extract by mixing and extracting pork potato, onion, Cudrania, bitter gourd, aronia and ginger; And (C) mixing the first mixed extract and the second mixed extract. The step of adding stevia may be added after step (C).

First, in the step (A), a mixture of thistle and dandelion is extracted with an extraction solvent to obtain a first mixed extract.

The mixing conditions, extraction solvent and extraction conditions of the mixture are the same as those mentioned above.

Next, in the step (B), a mixture of pork potato, onion, cudrania, bitter gourd, aronia, and ginger is extracted with an extraction solvent to obtain a second mixed extract.

The mixing conditions, extraction solvent and extraction conditions of the mixture are the same as those mentioned above.

When the first mixed extract is prepared by mixing thistle and dandelion in step (A), when extracting with pork potato, onion, cudrania, bitter gourd, aronia and ginger is also added and extracted, the bitter taste is strong and the prevention, treatment of diabetes, or Since the improving effect may be significantly reduced, it is preferable to mix thistle and dandelion to add a second mixed extract extracted by mixing pork potato, onion, cudrania, bitter gourd, aronia and ginger separately to the first mixed extract. .

Next, in the step (C), the first mixed extract and the second mixed extract are mixed in a weight ratio of 1: 1-5, preferably in a weight ratio of 1: 1-2.

If the content of the second mixed extract based on the first mixed extract is less than the lower limit, the organoleptic is lowered and the effect of preventing, treating or improving diabetes may be significantly lowered, and if it exceeds the upper limit, the prevention of diabetes, The treatment or improvement effect is not improved, and the effect is similar to that of each use of the above substances.

Stevia ( Stevia rebaudiana ) grows around rivers and wetlands. Stevia leaves have been used as a sweetener since ancient times. Recently, when the harmfulness of saccharin, a synthetic sweetener, became a problem, it attracted attention again. Leaves contain stevioside, a sweetening substance about 6-7% by weight, and the content varies greatly depending on the individual. The sweetening ingredient is 300 times that of sugar, and it is used as a sweetener in soft drinks and as a substitute for tea or chewing gum.

In the present invention, the use of stevia increases the functionality.

The stevia is mixed in an amount of 0.1 to 5 parts by weight, preferably 0.5 to 1 part by weight, based on 100 parts by weight of thistle.

If the content of stevia is out of the above range, the functionality may be lowered.

The form of the composition of the present invention may be prepared in the form of gels, purees, juices, pills, large rings, and granules.

For example, in the case of being prepared in the form of gel and puree, the mixture of the first mixed extract and the second mixed extract is concentrated to a volume of 1/3 to 1/5 of the volume of the mixture with a concentrator, and then agar is It is added, stirred, and then sterilized to prepare a gel and puree.

As another example, when prepared in the form of juice, the mixture obtained by mixing the first mixed extract and the second mixed extract is sterilized and used as it is.

As another example, in the case of manufacturing in the form of a pill, a large pill, and a granule, a mixture obtained by mixing the first mixed extract and the second mixed extract is concentrated to a volume of 1/5 to 1/7 relative to the volume of the mixture with a concentrator. Then, it is aged for 40 to 50 hours in a water bath at 60 to 70°C, then dried and molded to prepare rings, large rings, and granules.

The mixture of the first mixed extract and the second mixed extract of the present invention is, in a broad sense, a mixed product formulated to administer a mixture of the first mixed extract and the second mixed extract to an animal, such as a first mixed extract. It is meant to include a mixed powder obtained by mixing the mixed extract and the second mixed extract. Although the experiment was conducted with a mixture of the first mixed extract and the second mixed extract in the present invention, the desired effect can be achieved even in the same form as the mixed processed product mixed with the first mixed extract and the second mixed extract. It will be predictable to those skilled in the art.

Meanwhile, in the present specification, the term "containing as an active ingredient" means including an amount sufficient to achieve the efficacy or activity of the mixture of the first mixed extract and the second mixed extract. For example, a mixture of the first mixed extract and the second mixed extract is used at a concentration of 10 to 1500 µg/ml, preferably 100 to 1000 µg/ml. Since the mixture of the first mixed extract and the second mixed extract is a natural product and does not have side effects on the human body even if an excessive amount is administered, the upper limit of the quantity of the mixture containing the first mixed extract and the second mixed extract included in the composition of the present invention is a person skilled in the art. Can be carried out by selecting within an appropriate range.

The pharmaceutical composition of the present invention may be prepared using a pharmaceutically suitable and physiologically acceptable adjuvant in addition to the active ingredient, and the adjuvants include excipients, disintegrants, sweeteners, binders, coating agents, expanding agents, lubricants, Lubricating agents or flavoring agents can be used.

For administration, the pharmaceutical composition may contain one or more pharmaceutically acceptable carriers in addition to the above-described active ingredients, and may be preferably formulated into a pharmaceutical composition.

The formulation form of the pharmaceutical composition may be granules, powders, tablets, coated tablets, capsules, suppositories, solutions, syrups, juices, suspensions, emulsions, drops, or injectable solutions. For example, for formulation in the form of tablets or capsules, the active ingredient may be combined with an oral, non-toxic pharmaceutically acceptable inert carrier such as ethanol, glycerol, water, and the like. In addition, if desired or necessary, suitable binders, lubricants, disintegrants and coloring agents may also be included in the mixture. Suitable binders are, but are not limited to, natural sugars such as starch, gelatin, glucose or beta-lactose, corn sweeteners, natural and synthetic gums such as acacia, trackcacanth or sodium oleate, sodium stearate, magnesium stearate, sodium Benzoate, sodium acetate, sodium chloride, and the like. Disintegrants include, but are not limited to, starch, methyl cellulose, agar, bentonite, xanthan gum, and the like.

Acceptable pharmaceutical carriers for compositions formulated as liquid solutions are sterilized and biocompatible, and include saline, sterile water, Ringer's solution, buffered saline, albumin injection solution, dextrose solution, maltodextrin solution, glycerol, ethanol, and One or more of these components may be mixed and used, and other conventional additives such as antioxidants, buffers, and bacteriostatic agents may be added as necessary. In addition, diluents, dispersants, surfactants, binders, and lubricants may be additionally added to prepare injection formulations such as aqueous solutions, suspensions, emulsions, etc., pills, capsules, granules, or tablets.

Further, it can be preferably formulated according to each disease or ingredient using a method disclosed in Remington's Pharmaceutical Science, Mack Publishing Company, Easton PA as an appropriate method in the field.

The pharmaceutical composition of the present invention may be administered orally or parenterally, and in the case of parenteral administration, it may be administered by intravenous injection, subcutaneous injection, intramuscular injection, intraperitoneal injection, transdermal administration, etc., preferably oral administration. .

A suitable dosage of the pharmaceutical composition of the present invention varies depending on factors such as formulation method, mode of administration, age, weight, sex, pathological condition, food, administration time, route of administration, excretion rate and response sensitivity of the patient, Usually the skilled practitioner can readily determine and prescribe a dosage effective for the desired treatment or prophylaxis. According to a preferred embodiment of the present invention, the daily dosage of the pharmaceutical composition of the present invention is 0.001-10 g/kg.

The pharmaceutical composition of the present invention is prepared in unit dosage form by formulating using a pharmaceutically acceptable carrier and/or excipient according to a method that can be easily carried out by a person having ordinary knowledge in the art. Or it can be prepared by incorporating it into a multi-dose container. In this case, the formulation may be in the form of a solution, suspension or emulsion in an oil or aqueous medium, or may be in the form of an extract, powder, granule, tablet or capsule, and may additionally include a dispersant or a stabilizer.

In addition, the present invention provides a food composition for preventing, treating or improving diabetes containing a mixture of the first mixed extract and the second mixed extract as an active ingredient.

The food composition according to the present invention may be formulated in the same manner as the pharmaceutical composition and used as a functional food or added to various foods. Foods to which the composition of the present invention can be added include, for example, beverages, alcoholic beverages, confectionery, diet bars, dairy products, meat, chocolate, pizza, ramen, other noodles, gums, ice creams, vitamin complexes, health supplements. Etc.

The food composition of the present invention may include not only a mixture of the first mixed extract and the second mixed extract as an active ingredient, but also ingredients commonly added during food production, for example, proteins, carbohydrates, fats, Contains nutrients, flavoring and flavoring agents. Examples of the aforementioned carbohydrates include monosaccharides such as glucose, fructose, and the like; Disaccharides such as maltose, sucrose, oligosaccharides, and the like; And polysaccharides, for example, common sugars such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents, natural flavoring agents [taumatin, stevia extract (eg, rebaudioside A, glycyrrhizin, etc.]) and synthetic flavoring agents (saccharin, aspartame, etc.) can be used. For example, when the food composition of the present invention is made of drinks and beverages, in addition to the mixture of the first mixed extract and the second mixed extract of the present invention, citric acid, liquid fructose, sugar, glucose, acetic acid, malic acid, fruit juice, and various Plant extracts and the like may be further included.

The present invention provides a health functional food comprising a food composition for preventing, treating or improving diabetes comprising a mixture of the first mixed extract and the second mixed extract as an active ingredient. Health functional foods are foods prepared by adding a mixture of the first mixed extract and the second mixed extract to food materials such as beverages, teas, spices, gums, confectionery, etc., or by encapsulation, powdering, suspension, etc. When ingested, it means that it has a specific effect on health, but unlike general drugs, it has the advantage of not having side effects that may occur when taking the drug for a long time by using food as a raw material. The health functional food of the present invention obtained in this way is very useful because it can be consumed on a daily basis. In such health functional foods, the amount of the mixture of the first mixed extract and the second mixed extract may not be uniformly regulated depending on the type of the health functional food, but the range does not impair the original taste of the food. It may be added from, and is usually in the range of 0.01 to 50% by weight, preferably 0.1 to 20% by weight, based on the target food. In addition, in the case of health functional foods in the form of pills, granules, tablets or capsules, it is usually added in the range of 0.1 to 100% by weight, preferably 0.5 to 80% by weight. In one embodiment, the health functional food of the present invention may be in the form of a pill, tablet, capsule or beverage.

In addition, the present invention provides a use of a mixture obtained by mixing the first mixed extract and the second mixed extract for the manufacture of a drug or food for preventing, treating or improving diabetes. As described above, a mixture of the first mixed extract and the second mixed extract may be used for diabetes.

In addition, the present invention provides a method for improving, preventing or treating diabetes, comprising administering to a mammal an effective amount of a mixture of the first mixed extract and the second mixed extract.

The term "mammal" as used herein refers to a mammal that is an object of treatment, observation or experiment, and preferably refers to a human.

The term "effective amount" as used herein refers to the amount of an active ingredient or pharmaceutical composition that induces a biological or medical response in a tissue system, animal or human, which is considered by a researcher, veterinarian, doctor or other clinician, which Includes an amount that induces relief of symptoms of the disease or disorder. It is apparent to those skilled in the art that the effective amount and the number of administrations for the active ingredient of the present invention will vary depending on the desired effect. Therefore, the optimal dosage to be administered can be easily determined by those skilled in the art, and the type of disease, the severity of the disease, the content of active ingredients and other ingredients contained in the composition, the type of formulation, and the age, weight, and general health of the patient. It can be adjusted according to various factors including condition, sex and diet, time of administration, route of administration and rate of secretion of the composition, duration of treatment, and drugs used concurrently. In the prevention, treatment, or improvement method of the present invention, in the case of an adult, when a mixture of the first mixed extract and the second mixed extract is administered once to several times a day, a dose of 0.001 g/kg to 10 g/kg It is preferable to administer it.

In the treatment method of the present invention, a composition comprising a mixture of the first mixed extract and the second mixed extract as an active ingredient is oral, rectal, intravenous, intraarterial, intraperitoneal, intramuscular, intrasternal, transdermal, topical, It can be administered in a conventional manner via the intraocular or intradermal route.

Hereinafter, a preferred embodiment is presented to aid in the understanding of the present invention, but it is obvious to those skilled in the art that various changes and modifications are possible within the scope of the present invention and the scope of the technical idea, but the following examples are only illustrative of the present invention, It is natural that such modifications and modifications fall within the scope of the appended claims.

Manufacturing example 1 Preparation of thistle

1 part by weight of thistle flower, a mixture of stem and leaf (stem: leaf = 1:2 weight ratio) 18.6 parts by weight and 45 parts by weight of water were mixed and extracted in a bath for 24 hours at 95° C. to obtain extract A, and then thistle root 0.4 Part by weight and 45 parts by weight of water were extracted in a bath at 95° C. for 24 hours to obtain extract B, and then freeze-dried to prepare and mix powder A and powder B to prepare a mixed thistle extract powder.

Example One.

Preparation of the first mixed extract

After mixing 100 parts by weight of thistle and 41 parts by weight of dandelion prepared in Preparation Example 1, the mixture and water were mixed in a weight ratio of 1:9 and extracted at 90° C. for 48 hours to obtain a first mixed extract.

Preparation of the second mixed extract

Pork potato, onion, Cudrania tree, bitter gourd, aronia, and ginger were mixed at a weight ratio of 1: 1: 0.24: 0.24: 0.24: 0.024 at a weight ratio of mixing and extracted at 90° C. for 10 hours to obtain a second mixed extract. .

Preparation of the final mixed extract

The first mixed extract and the second mixed extract were mixed at a weight ratio of 1: 1.26 and mixed at room temperature for 10 minutes to obtain a final mixed extract.

Comparative Example 1. Using only the first mixed extract

It was carried out in the same manner as in Example 1, but only the first mixed extract was used without using the second mixed extract.

Comparative Example 2. Using only the second mixed extract

It was carried out in the same manner as in Example 1, but only the second mixed extract was used without using the first mixed extract.

Comparative Example 3. First mixed extract: second mixed extract = 1: 0.5 weight ratio

In the same manner as in Example 1, the first mixed extract and the second mixed extract were mixed at a weight ratio of 1:0.5 and mixed at room temperature for 10 minutes to obtain a final mixed extract.

<Test Example>

Experimental animals

6-week-old male db/db mice, which are obese and show diabetic symptoms, artificially cause genetic mutations to block the feedback signaling of leptin, thereby causing type 2 diabetes along with excess weight as they grow. As a result, the drug was orally administered to this mouse model, and the same amount of physiological saline was administered to the control group. The experimental group, db/db mice, was randomly assigned to 7 mice per group, and one group was given a vehicle (saline, negative control), a positive control group was given a banaba leaf extract (wellis banana) 100 mg/kg, and the experimental group was The sample prepared above was orally administered at 2 g/kg each day at 10 am and 18:00 pm (0.4 ml) (each sample was suspended in saline and added). The breeding conditions of each group are the same as in the preliminary animal experiments, while maintaining in an SPF environment controlled by temperature 22±2 ℃, humidity 55±10%, and contrast at 12 hour intervals, weight and blood sugar at 0, 7, 14, 21 days of administration. A portable blood glucose meter (OneTouch TM, Johnson & Johnson, USA) was used for the change, and the average value was shown for each experimental group.

Blood analysis

After administering each sample to db/db mice for 21 days, blood was collected from animals of each group, centrifuged at 3,000 rpm for 10 minutes, and then the supernatant plasma was separated and analyzed for glucose, etc. to determine the difference from the control group. Statistical method (student t-test)

Significance analysis was performed using.

Statistical processing

The results obtained from various experiments were recorded as mean ㅁ standard error, and significance verification was determined using Student's T-test analysis method.

Test Example 1. Measurement of anti-diabetic effect in a type 2 diabetic animal model mouse

In order to confirm the hypoglycemic effect of the sample of the above example, an experiment was performed in the following procedure by modifying the method disclosed in the literature [Am J Physiol Endocrinol Metab. 288: E510-E518 (2005); Diabetologia 49: 1647-1655 (2006); J. Ethnopharmacol. 103: 491-495 (2006); J. Med. Chem. 43: 3487-3494 (2003); Metabolism 50:1049-1053 (2001); Nutrition & Metabolism 53: 488-499 (2004)].

1-1. Effects on blood sugar changes

Each sample was orally administered to a 6-week-old db/db mouse model twice a day for 3 weeks in the morning and in the afternoon. Blood was collected from the tails of mice with a portable glucose meter once a week to measure blood sugar, and the day before the measurement, all mice The diet was removed (about 12 hours).

Category (Unit: blood sugar mg/dL) 0 days 7 days 14 days 21 days Negative control 350.8 396.7 574.9 598.2 Positive control 361.7 409.6 392.8 372.8 Example 1 359.0 385.7 375.1 364.8 Comparative Example 1 362.4 461.3 465.2 467.0 Comparative Example 2 361.9 443.9 448.7 440.1 Comparative Example 3 362.5 438.9 441.7 433.9

As shown in Table 1 above, it was confirmed that the mixture prepared according to Example 1 of the present invention exhibited excellent inhibition of blood sugar from 0 to 21 days.

On the other hand, in the negative control group and Comparative Example 1, blood sugar was not suppressed and was continuously increased, and it was confirmed that the blood sugar in Comparative Examples 2 and 3 was not suppressed compared to Example 1.

1-2. Measurement of changes in serum glucose (glucose)

On the 21st day, the day before the end of the experiment, the diet was removed, anesthetized with ethyl ether on an empty stomach (about 12 hours), blood was collected from the heart with a 3 ml syringe, left at room temperature for 1 hour, and then centrifuged at 3000 rpm for 10 minutes. The serum was separated, and the change of glucose in the serum was measured with an automatic serum measuring instrument.

division Glucose measurement (mg/dL) Negative control 631.9 Positive control 462.1 Example 1 474.5 Comparative Example 1 546.9 Comparative Example 2 536.9 Comparative Example 3 531.4

As shown in Table 2 above, it was confirmed that the mixture prepared according to Example 1 of the present invention decreased glucose compared to the negative control group and Comparative Examples 1 to 3.

As a result of Table 1 and Table 2, it was confirmed that the composition of Example 1 has anti-diabetic efficacy.

Test Example 2. Influence on body weight and diet change

The change in body weight was measured twice a week with 7 mice of each experimental group, and the average dietary intake of each experimental group (7 mice) was observed by measuring the dietary intake at 3 days intervals.

Weight change

division
(Unit: g) 0 days 0.5 days 7 days 7.5 days 14 days 14.5 days 21 days Negative control 29.1 29.4 29.9 30.8 31.8 32.6 33.9 Positive control 28.7 29.0 29.4 29.7 29.8 30.5 30.8 Example 1 28.9 29.2 29.6 29.9 30.2 30.4 30.6 Comparative Example 1 29.2 29.6 30.0 30.3 30.7 31.1 31.7 Comparative Example 2 28.5 29.0 29.5 30.0 30.4 30.9 31.4 Comparative Example 3 28.7 29.3 29.7 30.2 30.8 31.2 31.6

Dietary intake

division
(Unit: g) 7 days 14 days 21 days Negative control 33.3 38.1 38.6 Positive control 33.5 38.4 38.1 Example 1 33.3 38.3 38.7 Comparative Example 1 33.2 38.5 38.5 Comparative Example 2 33.5 38.8 38.7 Comparative Example 3 33.4 38.7 38.4

As shown in Tables 3 and 4 above, although the intake amount of the compound prepared according to Example 1 of the present invention was similar to that of the negative control group and Comparative Examples 1 to 3, the weight decreased slightly compared to the negative control group and Comparative Examples 1 to 3 Confirmed.

Test Example 3. Acute toxicity test

Acute toxicity experiments were conducted using 6-week-old specific pathogen-free (SPF) SD rats. The compositions of Examples and Comparative Examples were administered orally once at a dose of 100 mg/kg to 2 animals for each group. After administration of the test substance, the animals were observed for mortality, clinical symptoms, and body weight changes, hematological and blood biochemical tests were performed, and autopsies were performed to visually observe the abnormalities of tonic organs and thoracic organs.

As a result, there were no significant clinical symptoms or mortality in all animals administered the test substance, and no toxic changes were observed in weight change, blood test, blood biochemical test, and autopsy findings.

Hereinafter, examples of the formulation of the composition containing the powder of the present invention will be described, but the present invention is not intended to limit this, but is intended to be described in detail.

Formulation Example 1. Preparation of powder

500 mg of powder mixture obtained in Example 1

100 mg lactose

10 mg of talc

The above ingredients are mixed and filled in an airtight cloth to prepare a powder.

Formulation Example 2. Preparation of tablets

300 mg of powder mixture obtained in Example 1

100 mg corn starch

100 mg lactose

2 mg of magnesium stearate

After mixing the above ingredients, tablets are prepared by tableting according to a conventional tablet manufacturing method.

Formulation Example 3. Preparation of Capsule

200 mg of powder mixture obtained in Example 1

3 mg of crystalline cellulose

14.8 mg lactose

Magnesium stearate 0.2 mg

According to a conventional capsule preparation method, the above ingredients are mixed and filled into gelatin capsules to prepare a capsule.

Formulation Example 4. Preparation of injection

600 mg of powder mixture obtained in Example 1

Mannitol 180 mg

2974 mg of sterile distilled water for injection

Na ₂ HPO _4, 12H ₂ O 26 mg

It is prepared in the amount of the above ingredients per ampoule according to a conventional injection preparation method.

Formulation Example 5. Preparation of liquid formulation

4 g of powder mixture obtained in Example 1

10 g of isomerized sugar

5 g of mannitol

Purified water appropriate amount

According to the usual preparation method of liquid formulations, add and dissolve each component in purified water, add an appropriate amount of lemon scent, mix the above ingredients, add purified water and add purified water to adjust the total to 100 g, then fill in a brown bottle for sterilization. To prepare a liquid formulation.

Formulation Example 6. Preparation of granules

1,000 mg of powder mixture obtained in Example 1

The right amount of vitamin mixture

Vitamin A acetate 70 ㎍

1.0 mg of vitamin E

Vitamin B1 0.13 mg

Vitamin B2 0.15 mg

0.5 mg of vitamin B6

Vitamin B12 0.2 ㎍

10 mg of vitamin C

Biotin 10 ㎍

1.7 mg of nicotinic acid amide

Folic acid 50 ㎍

0.5 mg of calcium pantothenate

Suitable amount of inorganic mixture

Ferrous sulfate 1.75 mg

Zinc oxide 0.82 mg

Magnesium carbonate 25.3 mg

Potassium monophosphate 15 mg

Dicalcium phosphate 55 mg

90 mg of potassium citrate

100 mg of calcium carbonate

Magnesium chloride 24.8 mg

The composition ratio of the vitamin and mineral mixture is relatively suitable for granules, but it is also possible to arbitrarily modify the mixing ratio. After mixing the above ingredients according to a conventional granule preparation method, granules And, according to a conventional method, it can be used for preparing a health functional food composition.

Formulation Example 7. Preparation of functional beverage

1,000 mg of powder mixture obtained in Example 1

1,000 mg citric acid

100 g oligosaccharides

2 g of plum concentrate

1 g taurine

900 mL total by adding purified water

After mixing the above ingredients according to the normal health drink manufacturing method, stirring and heating the mixture at 85°C for about 1 hour, the resulting solution is filtered and obtained in a sterilized 2 L container, sealed and sterilized, and then stored in a refrigerator. It is used to prepare the functional beverage composition of the present invention.

The composition ratio is a mixture of ingredients suitable for a relatively preferred beverage in a preferred embodiment, but the mixing ratio may be arbitrarily modified according to regional and ethnic preferences such as the demand class, the country of demand, and the purpose of use.

Claims (7)

A first mixed extract obtained by extracting a mixture consisting of thistle and dandelion; And a second mixed extract obtained by extracting a mixture consisting of pork potato, onion, cudrania, bitter gourd, aronia, and ginger. Food composition for preventing or improving diabetes, characterized in that it contains as an active ingredient. The food composition for preventing or improving diabetes according to claim 1, wherein the first mixed extract is an extract extracted at 80 to 100°C by mixing with 25 to 55 parts by weight of dandelion based on 100 parts by weight of thistle. According to claim 1, wherein the second mixed extract is a weight ratio of pork potato, onion, Cudrania tree, bitter gourd, aronia and ginger 1: 0.8-1.2: 0.1-0.8: 0.1-0.8: 0.1-0.8: 0.01-0.05 Food composition for preventing or improving diabetes, characterized in that the extract extracted at 80 to 100 ℃ by mixing. The food composition for preventing or improving diabetes according to claim 1, wherein the first mixed extract and the second mixed extract are mixed in a weight ratio of 1: 1-5. The food composition for preventing or improving diabetes according to claim 1, wherein the thistle is a mixture of flowers of thistle, a mixture of stems and leaves, and roots in a weight ratio of 1: 14-25: 0.1-0.9. (A) mixing and extracting thistle and dandelion to obtain a first mixed extract;
(B) obtaining a second mixed extract by mixing and extracting pork potato, onion, cudrania, bitter gourd, aronia, and ginger; And
(C) mixing the first mixed extract and the second mixed extract; a method for preparing a food composition for preventing or improving diabetes, comprising. A first mixed extract obtained by extracting a mixture consisting of thistle and dandelion; And a second mixed extract obtained by extracting a mixture consisting of pork potato, onion, cudrania, bitter gourd, aronia, and ginger. A pharmaceutical composition for preventing or treating diabetes, characterized in that it contains as an active ingredient.