KR20210049227A - Anti-microbial composition containing Rosa multiflora extract as effective component - Google Patents

Abstract

The present invention relates to an anti-microbial composition containing a Rosa multiflora extract as an active ingredient, and the Rosa multiflora extract, which is an active ingredient of the present invention, shows an excellent effect of antibacterial activity on Escherichia coli, Staphylococcus aureus and Candida albicans compared to lavender, lemongrass, chamomile, Orostachys japonicus, Perilla ocymoides, dandelion, Ligularia stenocephala, Chrysanthemum indicum and Siberian chrysanthemum extracts. The antibacterial composition of the present invention can be usefully applied to cosmetics, health functional foods, detergents or pharmaceuticals.

Description

Anti-microbial composition containing Rosa multiflora extract as effective component}

The present invention relates to an antibacterial composition containing a brier extract as an active ingredient.

In general, direct or indirect damage caused by pathogenic microorganisms causes many economic, environmental and medical problems. In particular, various types of skin diseases are mainly caused by skin flora, and in the case of atopic dermatitis, the cause is not clearly identified, but staphylococcus has been reported as the pathogen. The use of preservatives and antibacterial agents is essential to reduce the occurrence of skin diseases and protect the skin caused by such skin flora and other bacteria, but synthetic antibiotics that are used in the past have a problem that causes strong skin irritation. There is a burden to use in the state. Accordingly, it is expected that natural antibacterial agents that are harmless to the human body can be developed by searching for antibacterial functions from plant extracts.

On the other hand, as a prior art related to the antibacterial effect of plant extracts, a composition for antibacterial comprising a honeysuckle extract and a black pepper extract is disclosed in Korean Patent Registration No. 2009604, and Schisandra chinensis extract is effective in Korean Patent Publication No. 2019-0006285. Although an antimicrobial composition containing as a component has been disclosed, no antimicrobial composition containing the brier extract of the present invention as an active ingredient has been disclosed.

The present invention has been derived by a request as described above, provides the antimicrobial for the compositions containing Briar extract as an active ingredient, and the composition of E. coli (Escherichia coli), Staphylococcus aureus (Staphylococcus aureus) and Candida albicans (Candida albicans ) by confirming that the antimicrobial activity is excellent, the present invention was completed.

In order to solve the above problems, the present invention provides an antibacterial composition containing a brier extract as an active ingredient.

In addition, the present invention provides a cosmetic comprising the antibacterial composition.

In addition, the present invention provides a health functional food comprising the antibacterial composition.

In addition, the present invention provides a cleaning agent comprising the antibacterial composition.

In addition, the present invention provides a pharmaceutical product comprising the antibacterial composition.

The present invention relates to an antibacterial composition containing a brier extract as an active ingredient, and the brier extract, which is an active ingredient of the present invention, is compared with lavender, lemongrass, chamomile, wasong, perilla leaf, dandelion, gondalbi, gamguk and Gujeolcho extract. ( Escherichia coli ), Staphylococcus aureus ( Staphylococcus aureus ) and Candida albicans ( Candida albicans ) has excellent antimicrobial activity.

Figure 1 is a result of confirming the antibacterial activity of 10 kinds of herbs (lavender, lemongrass, chamomile, wasong, perilla leaf, white dandelion, gondalbi, gamguk, gujeolcho and brier) water extract.
Figure 2 is a result of confirming the antibacterial activity of the ethanol extract of 10 kinds of herbs (lavender, lemongrass, chamomile, wasong, perilla leaf, white dandelion, gondalbi, gamguk, gujeolcho and brier).
3 is a result of confirming the antimicrobial activity of each concentration of Wasong ethanol extract (A), brier water extract (B) or brier ethanol extract (C).
Figure 4 is a solid antibacterial activity against the skin flora (E. coli , Staphylococcus aureus: S. aureus and Candida albicans: C. albicans ) of the Wasong water extract, Wasong ethanol extract, Brier water extract or Brier ethanol extract. This is the result confirmed on the medium.
Figure 5 is a result of confirming the antibacterial activity of 10 kinds of herbs (lavender, lemongrass, chamomile, wasong, perilla leaf, white dandelion, gondalbi, gamguk, gujeolcho and brier) 1,3-butylene glycol extract.
Figure 6 is a skin flora (E. coli , Staphylococcus aureus: S. aureus and Candida albicans) of 1,3-butylene glycol extract of 5 kinds of herbs (lavender, lemongrass, perilla leaf, gujeolcho and brier) : This is the result of confirming the antibacterial activity against C. albicans) in solid medium.
7 is a result of confirming the cytotoxicity (A), thermal stability (B) and light stability (C) of the brier 1, 3-butylene glycol extract.

The present invention relates to an antibacterial composition containing a brier extract as an active ingredient.

The brier extract was _{first extracted using water, C 1} ~ C ₄ lower alcohol or a mixture thereof as a solvent, and 1,3-butylene glycol was freeze-dried after the first extract was lyophilized. It may be secondary extraction using, and preferably, an extract obtained by primary extraction using water or ethanol as a solvent or an extract obtained by secondary extraction using 1,3-butylene glycol of a lyophilized product thereof, and most preferred For example, the first extract of brier water or ethanol is freeze-dried, and then the second extraction is performed using 1,3-butylene glycol, but is not limited thereto.

The antibacterial composition may further include one or more extracts selected from the group consisting of lavender, lemongrass, chamomile, wasong, perilla leaf, dandelion, gondalbi, Gamguk and Gujeolcho in addition to the brier extract, but is not limited thereto. .

In one embodiment of the present invention, the composition exhibits antimicrobial activity against one or more bacteria selected from the group consisting of Escherichia coli , Staphylococcus aureus , and Candida albicans.

In addition, the present invention relates to a cosmetic comprising the antibacterial composition.

The antibacterial composition contained in the cosmetic of the present invention is 0.0001 to 20% by weight, preferably 0.0001 to 10% by weight, and most preferably 0.0001 to 3.0% by weight, based on the total weight of the cosmetic product.

Ingredients included in the cosmetics of the present invention include ingredients commonly used in cosmetics in addition to the antibacterial composition as an active ingredient. For example thickeners, stabilizers, solubilizers, conventional auxiliaries such as vitamins, pigments and fragrances, and carriers and the like.

The cosmetic of the present invention can be prepared in any formulation conventionally prepared in the art. For example, it may be formulated as a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray, etc. It is not limited thereto.

Specifically, it may be prepared in the form of a flexible lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray, or a powder.

When the cosmetic formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide, calcium silicate as carrier components Alternatively, polyamide powder or the like may be used. In particular, in the case of a spray, a propellant such as chlorofluorohydrocarbon, propane/butane or dimethyl ether may be additionally included.

When the cosmetic formulation of the present invention is a solution or emulsion, a solvent, a solubilizing agent or an emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan.

When the cosmetic formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microparticles Crystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, and the like may be used.

When the cosmetic formulation of the present invention is a surfactant containing cleansing, as a carrier component, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid Amide ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters may be used.

In addition, the present invention relates to a health functional food comprising the antibacterial composition.

The health functional food of the present invention may be prepared in any one formulation selected from powders, granules, pills, tablets, capsules, candy, syrup, and beverages, but is not limited thereto.

When using the health functional food of the present invention as a food additive, the health functional food may be added as it is or may be used with other foods or food ingredients, and may be appropriately used according to a conventional method. The active ingredient can be appropriately used depending on the purpose of use (prevention or improvement). In general, when preparing food or beverage, the health functional food of the present invention is added in an amount of 15 parts by weight or less, preferably 10 parts by weight or less based on the raw material. However, in the case of long-term intake for the purpose of health, the amount may be less than the above range, and there is no problem in terms of safety, so the active ingredient may be used in an amount above the above range.

There is no particular limitation on the types of the health functional food. Examples of foods to which the health functional food can be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum, dairy products including ice cream, various soups, beverages, tea drinks. , Alcoholic beverages and vitamin complexes, and includes all health foods in the usual sense.

In addition, the health functional food of the present invention can be prepared as a food, particularly a functional food. Functional foods of the present invention may contain commonly added ingredients. Examples include proteins, carbohydrates, fats, nutrients and seasonings. For example, when prepared as a drink, natural carbohydrates or flavoring agents may be included as additional ingredients in addition to the active ingredient. The natural carbohydrates are monosaccharides (e.g., glucose, fructose, etc.), disaccharides (e.g., maltose, sucrose, etc.), oligosaccharides, polysaccharides (e.g., dextrins, cyclodextrins, etc.) or sugar alcohols (e.g. , Xylitol, sorbitol, erythritol, etc.). As the flavoring agent, natural flavoring agents (eg, taumatin, stevia extract, etc.) and synthetic flavoring agents (eg, saccharin, aspartame, etc.) may be used.

In addition to the above health functional foods, various nutrients, vitamins, electrolytes, flavoring agents, coloring agents, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonated beverages It may further contain a carbonation agent used in the. Although the ratio of the ingredients to be added is not very important, it is generally selected from 0.01 to 0.1 parts by weight based on 100 parts by weight of the health functional food composition of the present invention.

In addition, the present invention relates to a detergent comprising the antibacterial composition.

The cleaning agent refers to substances and products capable of removing or dispersing solid and liquid dirt from the surface being cleaned, and the cleaning refers to removing unwanted substances or microorganisms from the surface.

Ingredients contained in the detergent of the present invention include, as active ingredients, ingredients commonly used in detergents in addition to the antibacterial composition described above. For example, conventional auxiliaries such as fragrances, dyes, solvents, opacifying agents, copolymer-based dispersions, thickening agents, cellulose derivatives, natural gums, albumin derivatives, fats, oils, keratolytic agents, keratinizers, thickeners, stabilizers, and It includes a carrier and the like.

The detergent of the present invention can be used as a detergent for cleaning the human body, such as shampoo, conditioner, soap, hand wash, foam cleansing, etc., for cleaning the living environment such as tableware, bathroom, sink, and wallpaper. However, it is not limited thereto.

The detergent of the present invention can be prepared in any formulation conventionally prepared in the art. Examples include liquids, solids, creams, and sprays.

In addition, the present invention relates to a pharmaceutical product comprising the antibacterial composition.

The antibacterial composition contained in the pharmaceutical product of the present invention is 0.0001 to 30% by weight, preferably 0.0001 to 10% by weight, based on the total weight of the pharmaceutical composition.

The pharmaceuticals may be formulated into conventional formulations in the pharmaceutical field depending on the therapeutic purpose, for example, tablets, capsules, powders, granules, suspensions, emulsions, syrups, emulsions, warning agents, ointments, sprays, oils. It can be formulated as a preparation, a gel, an alcohol, a tincture, a bath, a liner, a lotion, a patch, a pad, a cream, and the like. In addition, it can be preferably used as an external preparation for skin for the purpose of topical administration applied directly to the affected area. In this case, an ointment, lotion, spray, gel, or the like is preferable. These external preparations for skin may also be included in a support base or matrix that can be applied directly to the treatment site, and examples of the support base include gauze or bandages. Plant extracts or fractions thereof used in the formulation may be in a colloidal form or a dried powder form.

When formulated as an ointment, the skin surface temperature, skin pH, transdermal water loss value, and total lipid value of the epidermis must be considered. Vaseline, liquid paraffin, paraffin, plastic base, silicone, pig paper (lard), vegetable oil, wax, oil-soluble substrates such as purified lanolin, water-soluble substrates, emulsion-based substrates, suspension substrates, etc. are formulated and formulated. These ointments are antioxidants (e.g., tocopherol, BHA, BHT, NDGA, etc.), preservatives (e.g., phenolic substances, chlorobutanol, benzyl alcohol, parabens, benzoic acid, etc.), moisturizing agents (e.g., glycerin, propylene clicone, sorbitol, etc.) ), solubility aids (eg, ethanol, propylene glycol), softening aids (eg, liquid paraffin, glycerin, propylene glycol, surfactants, etc.).

When formulated as a lotion, it can be prepared as a lotion, such as a solution type, a suspension type, or an emulsion type, and in the case of a lotion applied to the skin, it can be prepared to have a viscosity of, for example, 200 cps to 500 cps, and it feels soft when applied. It is preferable to prepare by blending a wetting agent such as glycerin and propylene glycol in order to give it.

When formulated as a spray, a propellant or the like may be blended with an additive so that the aqueous dispersion concentrate or wet powder is dispersed.

When formulated as a patch, an absorption accelerator may be used to increase the absorption of the compound through the skin.

The pharmaceutical products of the present invention can be administered by various methods of administration such as oral, parenteral, topical administration, rectal administration, and intranasal administration. In the case of oral administration, for example, oral solids can be used with active substances and diluents (e.g. lactose, dextrose, sucrose, cellulose, corn starch, potato starch, etc.), lubricants (e.g. silica, talc, stearic acid). , Magnesium or calcium stearate, polyethylene glycol, etc.), binders (e.g. starch, arabic gum, gelatin methylcellulose, carboxymethylcellulose, polyvinyl pyrrolidone, etc.), disintegrants (e.g. starch, alginic acid, alginate, etc.) Sodium starch glycolate, etc.), formal mixtures, dyes, sweeteners, wetting agents (eg, lecithin, polysorbate, lauryl sulfate, etc.), and other pharmacologically inert substances commonly used in pharmaceuticals. For topical administration, excipients (e.g. lactose, starch, cellulose, lactose, polyethylene glycol, etc.), lubricants (e.g. magnesium stearate, stearic acid, glyceryl behenate, talc, etc.), preservatives (e.g. benzyl) Alkonium chloride, etc.), and the like.

Parenteral administration includes injections that produce systemic effects or injections administered directly to the affected area. Examples of parenteral administration include subcutaneous, intravenous, intramuscular, intradermal, intrathecal, intraocular and general infusion techniques.

Topical administration includes treatment of easily accessible infected areas or organs by topical application, such as, for example, ear, vaginal, open and closed wounds or closed wounds and skin, including eye, outer and middle ear infections. It also includes transdermal delivery that results in systemic effects.

Rectal administration includes the form of suppositories.

Intranasal administration includes nasal aerosol or inhalation application.

The pharmaceutical product of the present invention is administered in a pharmaceutically effective amount. In the present invention, a pharmaceutically effective amount means an amount sufficient to treat or prevent a disease at a reasonable benefit/risk ratio applicable to medical treatment or prevention, and the effective dose level is the type of disease and its severity, drug activity, patient Age, weight, health and sex of the patient, sensitivity to the drug of the patient, the time of administration of the particular extract used, the route of administration and the rate of excretion, the duration of treatment, factors including drugs used in combination or co-use with the specific extract used, and other medicines. It can be determined according to factors well known in the field.

Hereinafter, the present invention will be described in more detail using Preparation Examples and Examples. These preparation examples and examples are only for describing the present invention in more detail, and it is obvious to those of ordinary skill in the art that the scope of the present invention is not limited thereto.

Preparation Example 1. Preparation of 10 kinds of herbal extracts

For lavender, outposts grown in Unbong-eup, Namwon-si, Jeollabuk-do, were collected and dried in July.

Lemongrass outposts grown in Ibaek-myeon, Namwon-si, Jeollabuk-do, were collected and dried in October.

For chamomile, flowers grown in Unbong-eup, Namwon-si, Jeollabuk-do, were collected and dried in May.

Wasong was collected and dried in October from outposts grown in Songdong-myeon, Namwon-si, Jeollabuk-do.

Perilla leaves were collected and dried in August from outposts grown in Jucheon-myeon, Namwon-si, Jeollabuk-do.

For white dandelion, outposts grown in Unbong-eup, Namwon-si, Jeollabuk-do, were collected and dried in April.

Gondalbi collected and dried outposts grown in Jucheon-myeon, Namwon-si, Jeollabuk-do in May.

The flowers grown in Ibaek-myeon, Namwon-si, Jeollabuk-do, were collected and dried in October.

For Gujeolcho, outposts grown in Sannae-myeon, Namwon-si, Jeollabuk-do, were collected and dried in October.

The brier was harvested and dried in May from outposts grown in Songdong-myeon, Namwon-si, Jeollabuk-do.

The 10 kinds of herbs were dried using a food dryer at 40°C for 72 hours, and the dried product was pulverized using a pulverizer (HMF-3600TG, Hanil, Seoul, Korea), and then 100 g of the pulverized product was quantified, Water or 50% (v/v) ethanol was added 20 times the amount of the pulverized product, followed by reflux extraction while heating. After that, the extract was first filtered using a nonwoven fabric, and then filtered second using a paper filter paper (Whatman NO. 3, Whatman NO. 5), and then a vacuum concentrator (R-100, BUCHI, Flawil, Switzerland). Then, it was concentrated at 50°C, and then lyophilized and used as a sample.

1,3-butylene glycol (1,3-butylene glycol) extract was used after dissolving the freeze-dried product of the herbal water or ethanol extract in 40% (v/v) 1,3-butylene glycol, followed by filtration.

Example 1. Confirmation of antibacterial effect of 10 kinds of herbal extracts

In order to measure the antibacterial activity against 10 kinds of herbal extracts, the antibacterial activity was confirmed by targeting 3 types of skin flora (Escherichia coli, Staphylococcus aureus, and Candida albicans).

As a result, as disclosed in FIG. 1, the brier water extract among the ten herbal water extracts was 70% for Staphylococcus aureus, 100% for E. coli, and 59% for Candida albicans when 5% (v/v) was treated. It showed the growth inhibition rate of, and showed the best antibacterial effect overall.

In addition, 10 kinds of herbal ethanol extracts as disclosed in FIG. 2 showed excellent antibacterial effects in the Wasong 2.5% (v/v) treatment group and the Briar 0.25% (v/v) treatment group, and the treatment concentration of the brier ethanol The antibacterial effect of the extract was generally excellent.

As a result of confirming the antibacterial effect of each treatment concentration of Wasong 50% (v/v) ethanol extract, brier water extract, and brier 50% (v/v) ethanol extract, which confirmed the excellent antibacterial effect in FIGS. 1 and 2, disclosed in FIG. 3 As shown, the antibacterial effect of the brier 50% (v/v) ethanol extract was the most excellent.

As a result of verifying the above results once more in a solid medium, the antibacterial effect of the brier ethanol extract was remarkable compared to the Wasong hot water extract, the Wasong ethanol extract, and the brier water extract, as disclosed in FIG. 4.

In addition, in order to use the herbal resource extract as a cosmetic material, as a result of confirming the antibacterial activity of the extract secondly extracted using 1,3-butylene glycol (1,3-BG), FIG. As disclosed, Brier 1,3-BG extract exhibited excellent antibacterial effect even at a low concentration of 6.25% (v/v), and solidified the antibacterial activity of lavender, lemongrass, perilla lobules, citrine and 1,3-BG extracts. As a result of verifying once more in the medium, the antibacterial activity was excellent in the brier 1,3-BG extract as disclosed in FIG. 6.

Example 2. Formulation safety and stability evaluation of brier 1,3-butylene glycol extract

Formulation safety and stability of the brier 1,3-butylene glycol extract showing excellent antibacterial effect were evaluated.

As a result, as disclosed in FIG. 7, when the 1,3-butylene glycol extract was treated on HDFn (Human Dermal Fibroblasts, neonatal) cells, which are human skin cells, cytotoxicity was reduced to a concentration of 2% (v/v). By confirming that it did not appear, it was confirmed that the brier 1,3-butylene glycol extract was a safe material without cytotoxicity.

In addition, there was no change in the formulation even when heat was applied to 4~60℃, and there was no change in properties even when irradiated with UVB 1.2~7.2J/㎠, indicating that the change of the extract due to light during long-term storage would be insignificant. Confirmed.

Claims (9)

Antibacterial composition containing brier extract as an active ingredient. The antibacterial composition of claim 1, wherein the brier extract is _{first extracted using water, C 1} ~ C ₄ lower alcohol or a mixture thereof. The antimicrobial composition according to claim 2, wherein the brier extract is a secondary extract of brier water or ethanol primary extract using 1,3-butylene glycol. The method of claim 1, wherein the composition further comprises one or more extracts selected from the group consisting of lavender, lemongrass, chamomile, wasong, perilla leaf, dandelion, gondalbi, gamguk and Gujeolcho in addition to the brier extract. Antibacterial composition. According to claim 1, wherein the composition is E. coli ( Escherichia coli ), Staphylococcus aureus ( Staphylococcus aureus ) and Candida albicans ( Candida albicans ) for antibacterial, characterized in that it exhibits antibacterial activity against one or more bacteria selected from the group consisting of Composition. Cosmetics comprising the antibacterial composition of claim 1. Health functional food comprising the antibacterial composition of claim 1. A detergent comprising the antibacterial composition of claim 1. A pharmaceutical product comprising the antibacterial composition of claim 1.

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