KR102260228B1 - Antibacterial composition containing extract of kenaf seed as effective component - Google Patents

Abstract

The present invention relates to an antibacterial composition containing a kenaf seed extract as an active ingredient, wherein the kenaf seed extract contains Escherichia coli , Bacillus cereus , Bacillus subtilis, compared to the kenaf leaf extract. ), Staphylococcus aureus has a remarkable antibacterial activity against. Therefore, the antibacterial composition of the present invention can be usefully applied to health functional foods, detergents, pharmaceuticals or cosmetics.

Description

Antibacterial composition containing extract of kenaf seed as effective component

The present invention relates to an antibacterial composition comprising a kenaf seed extract as an active ingredient.

In general, direct or indirect damage caused by pathogenic microorganisms causes many economic, environmental and medical problems. In particular, the loss due to spoilage during the food distribution process in the food industry, the harmfulness to the human body and environmental pollution due to the use of excessive chemical pesticides on crops in agriculture, and the emergence of antibiotic-resistant strains due to the misuse of antibiotics have already occurred in society. It is becoming a chronic problem in general. In this reality, natural physiologically active substances isolated from plants, etc. exhibit antibiotic activity in a wide range of pathogenic bacteria and fungi, but development as a new alternative material is required in that they can be used environmentally friendly.

In addition, most of the currently used antibiotics are manufactured through chemical synthesis, are expensive, and have many limitations, such as causing contamination and side effects as described above. Therefore, recently, research to isolate new antibacterial substances from natural products has been actively conducted, and the development of environmentally friendly substances capable of replacing the function of antibiotics is an important task.

Meanwhile, kenaf (scientific name: Hibiscus Cannabinus L.) is an annual herbaceous plant of the Malvaceae family, native to West Africa. As a result of a study on plants, it was designated as the most suitable plant as an alternative non-wood fiber for pulp and paper. Kenaf grows to a size of 4-5 m after 4-5 months of sowing and can be used as a paper resource, survives in poor dry soil or wet wetland, and has salt resistance, dry resistance, and heavy metal resistance. After sowing, it grows well with only the initial weeding and watering after sowing, and has the convenience of cultivation that is difficult to reach. In addition, kenaf absorbs 400 g of carbon dioxide per plant during cultivation, so it has a much higher carbon dioxide absorption capacity than general trees.

These kenafs contain an excess of tannins, saponins, polyphenols, alkaloids, and steroid compounds, and are known to be used in folk remedies in India and Africa for the treatment of sore throat disorders, bruises, over-regulation of bile segregation, fever and childbirth.

On the other hand, Korean Patent Application Laid-Open No. 2011-0092482 discloses a kenaf leaf extract with increased antibacterial activity by drying far-infrared rays and a method for preparing the same, and Korean Patent Publication No. 2019-0079875 discloses an antibacterial using Miseon tree extract as an active ingredient Although the composition has been disclosed, there is no disclosure regarding an antibacterial composition containing the kenaf seed extract of the present invention as an active ingredient.

The present invention has been derived from the above needs, and provides a kenaf leaf or seed extract, and Escherichia coli , Pseudomonas aeruginosa , Bacillus cereus of the kenaf leaf or seed extract , Bacillus subtilis ), Staphylococcus epidermidis ) and Staphylococcus aureus by comparing the antibacterial activity against, Escherichia coli of the Kenaf seed extract, Bacillus cereus ( Bacillus cereus ), Bacillus subtilis ( Bacillus subtilis ), Staphylococcus aureus ) By confirming that the antibacterial activity against kenaf leaf extract was remarkable, the present invention was completed.

In order to solve the above problems, the present invention provides an antibacterial composition containing a kenaf seed extract as an active ingredient.

In addition, the present invention provides a health functional food comprising the antibacterial composition.

In addition, the present invention provides a detergent comprising the antibacterial composition.

In addition, the present invention provides a pharmaceutical product comprising the antibacterial composition.

In addition, the present invention provides a cosmetic comprising the antibacterial composition.

The present invention relates to an antibacterial composition containing a kenaf seed extract as an active ingredient, wherein the kenaf seed extract contains Escherichia coli , Bacillus cereus , Bacillus subtilis, compared to the kenaf leaf extract. ), Staphylococcus aureus has a remarkable antibacterial activity against.

1 is a result confirming the antioxidant activity of the ethanol extract of kenaf seeds of the present invention. ETH is the kenaf seed ethanol extract treatment group.

The present invention relates to an antibacterial composition comprising a kenaf seed extract as an active ingredient.

The extraction solvent of the kenaf seed extract may be water, a C ₁ to C ₄ lower alcohol, or a mixture thereof, and is preferably extracted using ethanol, but is not limited thereto.

The kenaf seed extract exhibits antibacterial activity against one or more bacteria selected from the group consisting of Escherichia coli , Bacillus cereus , Bacillus subtilis , and Staphylococcus aureus.

The kenaf seed extract has antioxidant activity, but is not limited thereto.

In addition, the present invention relates to a health functional food comprising the antibacterial composition.

The health functional food of the present invention may be prepared in any one formulation selected from powder, granule, pill, tablet, capsule, candy, syrup and beverage, but is not limited thereto.

When the health functional food of the present invention is used as a food additive, the health functional food may be added as it is or may be used together with other foods or food ingredients, and may be appropriately used according to a conventional method. The active ingredient may be used appropriately depending on the purpose of its use (prevention or improvement). In general, in the production of food or beverage, the health functional food of the present invention is added in an amount of 15 parts by weight or less, preferably 10 parts by weight or less based on the raw material. However, in the case of long-term intake for health purposes, the amount may be less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount greater than the above range.

There is no particular limitation on the type of the health functional food. Examples of foods to which the health functional food can be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gums, dairy products including ice cream, various soups, beverages, tea drinks , alcoholic beverages and vitamin complexes, and includes all health foods in the ordinary sense.

In addition, the health functional food of the present invention can be prepared as a food, in particular, a functional food. The functional food of the present invention may include ingredients that are commonly added. Examples include proteins, carbohydrates, fats, nutrients and seasonings. For example, when manufactured as a drink, natural carbohydrates or flavoring agents may be included as additional ingredients in addition to the active ingredient. The natural carbohydrates include monosaccharides (eg, glucose, fructose, etc.), disaccharides (eg, maltose, sucrose, etc.), oligosaccharides, polysaccharides (eg, dextrin, cyclodextrin, etc.) or sugar alcohols (eg, , xylitol, sorbitol, erythritol, etc.). As the flavoring agent, natural flavoring agents (eg, taumatine, stevia extract, etc.) and synthetic flavoring agents (eg, saccharin, aspartame, etc.) may be used.

In addition to the above health functional food, various nutrients, vitamins, electrolytes, flavoring agents, coloring agents, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonated beverages It may further contain a carbonation agent, etc. used for The ratio of these added ingredients is not very important, but is generally selected in the range of 0.01 to 0.1 parts by weight based on 100 parts by weight of the health functional food composition of the present invention.

In addition, the present invention relates to a detergent comprising the antibacterial composition.

The cleaning agent refers to substances and products capable of removing or dispersing solid and liquid soil from a surface being cleaned, and the cleaning refers to removing unwanted substances or microorganisms from the surface.

The ingredients included in the detergent of the present invention include ingredients commonly used in detergents in addition to the antibacterial composition as an active ingredient. customary adjuvants such as, for example, fragrances, dyes, solvents, opacifying agents, copolymer-based dispersions, thickening agents, cellulose derivatives, natural gums, albumin derivatives, fats, oils, keratolytic agents, keratinizing agents, thickeners, stabilizers, and carriers and the like.

The detergent of the present invention can be used as a detergent for washing the human body, such as shampoo, conditioner, soap, hand wash, foam cleansing, etc., as a detergent for washing living environments such as tableware, bathroom, sink, wallpaper, etc. However, it is not limited thereto.

The detergent of the present invention may be prepared in any formulation conventionally prepared in the art. Examples include liquids, solids, creams, and sprays.

In addition, the present invention relates to a pharmaceutical product comprising the antibacterial composition.

The antibacterial composition contained in the pharmaceutical composition of the present invention is 0.0001 to 30% by weight, preferably 0.0001 to 10% by weight, based on the total weight of the pharmaceutical composition.

The pharmaceutical may be formulated as a conventional formulation in the pharmaceutical field according to the therapeutic purpose, for example, tablets, capsules, powders, granules, suspensions, emulsions, syrups, emulsions, warning agents, ointments, sprays, oils It may be formulated as a preparation, gel, alcohol, tincture, bath, liniment, lotion, patch, pad, cream, and the like. In addition, it can be preferably used as an external preparation for the skin for the purpose of topical administration applied directly to the affected area, in this case, the form of an ointment, lotion, spray, gel, etc. is preferable. These external preparations for skin may also be included in a support base or matrix that can be directly applied to the treatment site, and examples of the support base include gauze or a bandage. The plant extract or fractions thereof used for the formulation may be in a colloidal form or a dried powder form.

When formulating as an ointment, the skin surface temperature, skin pH, transdermal water loss value, total lipid value of the epidermis, etc. should be considered, and Vaseline, liquid paraffin, paraffin, plastic base, silicone, pork lard (lard), vegetable oil, wax, an oily base such as purified lanolin, a water-soluble base, an emulsion base, a suspension base, and the like. These ointments include antioxidants (eg tocopherol, BHA, BHT, NDGA, etc.), preservatives (eg phenolic substances, chlorobutanol, benzyl alcohol, parabens, benzoic acid, etc.), humectants (eg glycerin, propylene glycol, sorbitol). ), dissolution aids (eg, ethanol, propylene glycol), and softening aids (eg, liquid paraffin, glycerin, propylene glycol, surfactant, etc.) may be combined with additives.

When formulated as a lotion, it may be prepared as a lotion such as a solution type, a suspension type, or an emulsion type, and in the case of a lotion applied to the skin, it may be prepared to have a viscosity of, for example, 200cps to 500cps, and a soft feeling when applied It is preferable to prepare by mixing a wetting agent such as glycerin and propylene glycol in order to give it.

In the case of formulation as a spray, a propellant or the like may be blended with additives to disperse the dispersed concentrate or wet powder.

When formulated as a patch, an absorption enhancer may be used to increase absorption of the compound through the skin.

The pharmaceuticals of the present invention may be administered by various administration methods such as oral, parenteral, topical administration, rectal administration, and intranasal administration. For oral administration, for example, oral solids may be formulated with the active material as a diluent (eg, lactose, dextrose, sucrose, cellulose, corn starch, potato starch, etc.), lubricating agents (eg, silica, talc, stearic acid). , magnesium or calcium stearate, polyethylene glycol, etc.), binders (e.g. starch, gum arabic, gelatin methylcellulose, carboxymethylcellulose, polyvinyl pyrrolidone, etc.), disintegrants (e.g. starch, alginic acid, alginate, sodium starch glycolate, etc.), formal mixtures, dyes, sweeteners, wetting agents (eg, lecithin, polysorbate, lauryl sulfate, etc.), other commonly used pharmacologically inert substances in pharmaceuticals, and the like. For topical administration, excipients (eg, lactose, starch, cellulose, lactose, polyethylene glycol, etc.), lubricants (eg, magnesium stearate, stearic acid, glyceryl behenate, talc, etc.), preservatives (eg, benzyl) alkonium chloride, etc.) and the like.

Parenteral administration includes injections that produce a systemic effect or injections administered directly to the affected area. Examples of parenteral administration include subcutaneous, intravenous, intramuscular, intradermal, intrathecal, intraocular and general infusion techniques.

Topical administration includes, for example, treatment of infected areas or organs readily accessible by topical application such as the ear, vagina, open and closed wounds or closed wounds and skin, including eye, outer and middle ear infections. Also includes transdermal delivery which results in a systemic effect.

Rectal administration includes the form of suppositories.

Intranasal administration includes nasal aerosol or inhalation application.

The pharmaceutical of the present invention is administered in a pharmaceutically effective amount. In the present invention, a pharmaceutically effective amount means an amount sufficient to treat or prevent a disease at a reasonable benefit/risk ratio applicable to medical treatment or prevention, and the effective dose level is determined by the type and severity of the disease, the activity of the drug, the patient age, weight, health and sex of the patient, the patient's sensitivity to the drug, the time of administration of the particular extract used, the route and rate of excretion, the duration of treatment, factors including drugs used in combination with or concurrently with the particular extract used, and other medicines It may be determined according to factors well known in the art.

The present invention also relates to a cosmetic comprising the antibacterial composition.

The antibacterial composition contained in the cosmetic of the present invention is 0.0001 to 20% by weight, preferably 0.0001 to 10% by weight, and most preferably 0.0001 to 3.0% by weight based on the total weight of the cosmetic.

The ingredients included in the cosmetic of the present invention include ingredients commonly used in cosmetics in addition to the antibacterial composition as an active ingredient. customary adjuvants such as, for example, thickeners, stabilizers, solubilizers, vitamins, pigments and fragrances, and carriers and the like.

The cosmetic of the present invention may be prepared in any formulation conventionally prepared in the art. For example, it can be formulated as a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray, etc. The present invention is not limited thereto.

Specifically, it may be prepared in the form of a flexible lotion, a nourishing lotion, a nourishing cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray, or a powder.

When the cosmetic formulation of the present invention is a paste, cream or gel, as a carrier component, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide, calcium silicate Alternatively, polyamide powder or the like may be used, and in particular, in the case of a spray, a propellant such as chlorofluorohydrocarbon, propane/butane or dimethyl ether may be additionally included.

When the cosmetic formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan.

When the cosmetic formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester; Crystalline cellulose, aluminum metahydroxide, bentonite, agar or tracanth may be used.

When the cosmetic formulation of the present invention is a surfactant-containing cleansing agent, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid as carrier ingredients Amide ether sulfates, alkylamidobetaines, fatty alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters can be used.

Hereinafter, the present invention will be described in more detail using Preparation Examples and Examples. These preparations and examples are only for illustrating the present invention in more detail, and it is obvious to those of ordinary skill in the art that the scope of the present invention is not limited thereto.

Preparation Example 1. Preparation of Kenaf Extract

Kenaf leaves and seeds were provided by Kenaf (www.kenaf.net). The provided leaves and seeds were washed and dried in an oven at 55° C. for 1 week. Thereafter, the dried leaves and seeds were crushed using a pin crusher (JIC-P10-2; Myeongseong Machinery, Seoul, Korea). The crushed leaf and seed powder was passed through a sieve of 300 μm mesh size.

After immersing 800 g of kenaf leaf and seed powder passed through a sieve in 1.8 L of ethanol, it was extracted with stirring in a rotary shaker (VTRS-1 model, Nunes Instruments, Tamil Nadu, India) at room temperature for 7 days. After 7 days, the solvent extract was filtered through a sterile cotton plug, and then filtered a second time using Whatman filter paper No. 1, and then evaporated through a rotary evaporator at room temperature to obtain a semi-solid kenaf leaf extract. (56 g) and seed extract (61 g) were obtained. After that, the obtained sample was stored at 4°C.

Example 1. Antibacterial activity of kenaf extract

The antibacterial activity of the kenaf extract of Preparation Example 1 was analyzed through the disk diffusion method.

Nutrient agar was used under laminar air flow to coagulate in Petri dishes.

Gram-negative bacteria Escherichia coli ATCC 8739, Pseudomonas aeruginosa ATCC 9027, Gram-positive bacteria Bacillus cereus 14579, Bacillus subtilis ATCC 6633, Staphylococcus epidermidis, Staphylococcus epidermidis After culturing Staphylococcus aureus ATCC 6538, ^{100 μl of the strain inoculum adjusted to 10 8} CFU/mL was spread on a Petri dish with agar medium and inoculated.

After absorbing 10 mg/mL of the kenaf sample of Preparation Example 1 into each sterilized dry disk (diameter 8 mm), it was attached to the Petri dish, and the extract was diffused at room temperature for 1 hour. Then, after incubation at 37 ℃ for 24 hours, the diameter of the bacterial growth inhibition zone was measured in mm. As a positive control, kanamycin (30 μg/mL) was used.

As a result, as shown in Table 1 below, the kenaf leaf extract showed antibacterial activity against E. coli and Staphylococcus aureus, and the kenaf seed extract showed antibacterial activity against E. coli, Bacillus cereus, Bacillus subtilis and Staphylococcus aureus. In addition, in all strains with confirmed antibacterial activity, the antibacterial activity was remarkable when treated with the seed extract compared to kenaf leaves.

Antibacterial activity of kenaf extract zone of inhibition(mm) strain Gram-negative bacteria Gram-positive bacteria strain name coli Pseudomonas aeruginosa Bacillus cereus Bacillus subtilis Staphylococcus epidermis Staphylococcus aureus part leaf strain leaf strain leaf strain leaf strain leaf strain leaf strain Kenaf extract (10 mg/mL) 10.60
±
1.23 12.40
±
0.89 ^* - - - 12.50
±
0.63 - 15.10
±
0.97 - - 9.20
±
0.82 11.30
±
0.57 ^* kanamycin 28.3
±
0.98 29.6
±
0.71 31.2
±
0.44 30.1
±
0.87 26.4
±
0.52 27.7
±
0.38 31.3
±
0.32 26.1
±
0.83 28.2
±
0.11 30.5
±
0.54 25.7
±
0.48 27.1
±
0.51

In Table 1, * means that the antibacterial effect of the seed is statistically significant compared to the leaf, and p<0.05.

Example 2. Antioxidant activity of kenaf seed extract

The antioxidant activity of the kenaf seed extract, which was confirmed to have excellent antibacterial activity in Example 1, was evaluated as a free radical, 1,1-diphenyl-2-picrylhydrazyl (1,1-diphenyl-2-picrylhydrazyl, DPPH). ) was investigated using a method for measuring antioxidant activity (Braca et al.).

3 mL of 0.004% (w/v) DPPH diluted with methanol was mixed with 0.5 mL of the sample, vortexed, and reacted for 30 minutes at room temperature (25±5° C.) protected from light. Then, the absorbance was measured at 517 nm using a spectrophotometer (UV-1800 240V, Shimadzu Corporation, koyoto, Japan), and the measured value was compared to Equation 1 below to confirm the DPPH radical scavenging ability.

[Equation 1]

DPPH radical scavenging ability (%)=[(Abs _{c -Abs s} )Abs _c ]×100

Abs _c : Absorbance of DPPH radical + methanol mixture

Abs _s : Absorbance of DPPH radical + sample (extract or standard) mixture

As a result, as shown in FIG. 1 , it was confirmed that the radical scavenging activity of the ethanol extract was increased by about 40% compared to the sample untreated group.

Claims (8)

An antibacterial composition for one or more bacteria selected from the group consisting of Bacillus cereus , Bacillus subtilis and Staphylococcus aureus containing kenaf seed ethanol extract as an active ingredient. delete delete The antibacterial composition according to claim 1, wherein the kenaf seed extract has antioxidant activity. A health functional food comprising the antibacterial composition of claim 1. A detergent comprising the antibacterial composition of claim 1. A pharmaceutical comprising the antibacterial composition of claim 1. A cosmetic comprising the antibacterial composition of claim 1.

Images