KR20210033399A - Cosmetic Composition Comprising High Molecular Weight Sericin - Google Patents

Abstract

The present invention provides a stable oil-in-water emulsified cosmetic composition without precipitation and agglomeration even including high molecular weight sericin insoluble in purified water at room temperature by increasing the solubility of high molecular weight sericin using a polysaccharide polymer. An object of the present invention is to provide an oil-in-water cosmetic composition manufactured by dissolving polymer sericin and a method for manufacturing the same.

Description

Cosmetic Composition Comprising High Molecular Weight Sericin

The present invention relates to a cosmetic composition containing a sericin protein. More specifically, the present invention relates to a cosmetic composition stably containing high molecular weight sericin extracted from silkworm cocoon or silk silk.

Sericin (Sericin) is one of the light protein (硬蛋白質), also known as gonggyo (絹膠) and silk glue (silk glue), together with fibroin is a protein that makes up the cocoon fibers. Cocoon fiber is covered with two strands of fibroin and three layers of sericin. The rough and hard feeling of raw silk is because this sericin is attached, and when refined, it melts and disappears, giving it the unique texture of silk. In addition, the amino acid composition of sericin is characterized in that the ratio of polar amino acids such as Ser, Asp, and Thr occupies more than 60%. Such amino acid composition is similar to the distribution of proteins constituting human skin, and sericin is known to have various skin effects such as whitening, anti-inflammatory, and collagen synthesis ability.

Sericin has previously been discarded as a waste liquid in the process of refining silk, but research is being actively conducted to use it as a functional material in the fields of new biomaterials, cosmetics, and food. Existing methods used for refining sericin include various methods such as alkali refining, high temperature and high pressure treatment, and enzyme treatment. In the case of the commonly used alkali refining method, refining wastewater for the purpose of disposal is used as a material, and in order to recover sericin from it, a separate process is required to filter out the surfactant or base remaining in the refining wastewater. The efficiency is low, and it is difficult to recover pure sericin. In general, sericin obtained by using an alkali refining method or an enzyme treatment method is hydrolyzed to have a low molecular weight of about 15 kDa or less, and since such a low-molecular type sericin is easily soluble in water, it is used in cosmetics and food fields.

Throughout this specification, a number of documents are referenced and citations are indicated. The disclosure contents of the cited documents are incorporated by reference in the present specification as a whole, and the level of the technical field to which the present invention belongs and the contents of the present invention are more clearly described.

Korean Patent Publication No. 10-2033073

An object of the present invention is to provide an oil-in-water cosmetic composition prepared by dissolving a polymeric sericin and a method for preparing the same.

Another object of the present invention is to provide a cosmetic composition and a method for producing the same, which stably dissolves a polymer sericin having a low solubility in water, thereby smoothly and smoothly, and improves absorption.

Still other objects and advantages of the present invention will become more apparent by the following detailed description, claims, and drawings.

One aspect of the present invention is to provide a cosmetic composition comprising a polysaccharide polymer and a polymer sericin.

Sericin is known to have effects such as whitening, anti-inflammatory, and collagen synthesis, and it can have a surfactant ability due to a high proportion of polar amino acids, and the hydrophilic portion (Ser, Asp, Arg) of sericin reacts with the aqueous solution portion, and the hydrophobic portion (Gly, Tyr, Val) reacts with the oil layer to lower the interfacial tension. As the molecular weight increases, the hydrophilic and hydrophobic portions increase, and the amphoteric property increases, and the property of adsorbing to the interface increases. This indicates that sericin can be used as a surfactant, and when it is adsorbed to the interface to form an emulsion, sericin helps the formation of small particles and increases stability. The known HLB value of sericin is about 18 and can be used as an emulsifier for O/W formulations. In addition to the emulsifying ability, in general, when a polymer raw material is dissolved in water and used in a formulation, the adhesion and moisturizing feeling are often increased, and sericine can also be expected to improve the feeling of use.

However, unlike low-molecular sericin that dissolves in water at room temperature, high molecular weight sericin having an average molecular weight of 25 kDa or more has a problem in that it is difficult to apply to cosmetics because its solubility in water is low.

In particular, when sericin is subjected to high temperature and high pressure treatment using water as a solvent, an aqueous solution of polymeric sericin having an average molecular weight of 50,000 or more can be obtained. In such an aqueous solution, the solubility of sericin in water is poor, and the aqueous solution becomes a gel suspended at 0 to 40°C. In addition, if this is freeze-dried, sericin in powder form can be obtained. When the obtained sericin is dissolved in water again, the solubility decreases and it is not easily dissolved. Due to such a decrease in solubility, aggregation and sedimentation of sericin occurs, and the uniformity of the aqueous solution decreases. Therefore, it can be said that the improvement of solubility is essential to utilize the polymeric sericin in an aqueous solution form.

Accordingly, the present inventors provide a cosmetic composition of an oil-in-water emulsion formulation stably containing a polymer sericin by remarkably increasing the solubility of a high molecular weight sericin, preferably a high molecular weight sericin having a molecular weight of 25 kDa to 500 kDa, using a polysaccharide polymer. Reached. More specifically, by dissolving the polymeric sericin through a polysaccharide polymer, a composition having improved spreadability and smoothness and improved absorption was prepared.

Polymeric sericin in the composition of the present invention is a protein having an average molecular weight of 25 kDa or more, and a molecular weight of 50 kDa or more, 100 kDa or more, 150 kDa or more, 200 kDa or more, 250 kDa or more, 300 kDa or more, 350 kDa or more, 400 kDa or more , 450 kDa or more, or 500 kDa or more.

Conventionally, in cosmetic formulations, polysaccharide polymers have been used to improve moisturizing and nutritional sensation or to improve viscosity, but it has not been known that polysaccharide polymers can be used to dissolve polymeric sericin, which is insoluble in purified water at room temperature, in purified water.

Xanthan gum, one of the representative polysaccharide polymers, is a water-soluble polymer and is extracted from glucose or corn syrup. Xanthan gum has excellent heat resistance and acid resistance, and the viscosity of xanthan gum decreases when acid or alkali substances coexist with general thickeners, whereas xanthan gum has little change in viscosity due to pH. In addition, polysaccharide polymers such as cellulose gum and alginic acid may be used. As described above, since the polysaccharide polymer of the present invention can adjust the viscosity of the formulation at the same time as the polymer sericin is dissolved, it can be very usefully used in preparing an O/W-type emulsion formulation.

Specifically, the polysaccharide polymer used in the present invention is gum, alginic acid, sodium alginate, calcium alginate, starch, agar carrageenan, cellulose polymer, gellan, pectin, dextran (DEXTRAN), glucan (GRUCAN), glucomannan (GLUCOMANNAN), arabino galactan (ARABINO GALACTAN), purcelleran (FURCELLERAN), pullulan (PULLULAN), glucosamine (GLUCOSAMINE) and gelatin (GELATIN) one or more may be selected from the group consisting of, but not limited to .

The gums are xanthan gum, dehydroxanthan gum, cellulose gum, hydrolyzed cellulose gum, carrageenan gum, guar gum, benzoin gum (Styrax Benzoin Gum), sclerotium gum. (Sclerotium Gum), Biosaccharide Gum, Arabic Gum, LOCUST BEAN GUM, Tamarind Gum, GHATTI GUM, Ceratonia Siliqua Gum ), Tragalus Gummifer Gum, Hydrolyzed Rhizobian Gum, Sclerotium Gum, and the like, but are not limited thereto.

The cellulose-based polymer is calcium carboxymethyl cellulose, carboxymethyl cellulose acetate butyrate, carboxymethyl hydroxyethylcellulose, cellulose acetate, and cellulose acetate butyrate. Acetate Butyrate), Cellulose Acetate Propionate, Cellulose Acetate Propionate Carboxylate, Cellulose Succinate, Cetyl Hydroxyethylcellulose, Ethyl Cellulose (Ethylcellulose), Hydroxybutyl Methylcellulose, Hydroxyethylcellulose, Hydroxyethyl Ethylcellulose, Hydroxypropylcellulose, Hydroxypropyl Methylcellulose, Methylcellulose, Hydroxypropyl Methylcellulose Acetate/Succinate, Methylcellulose, Methyl Ethylcellulose, Methyl Hydroxyethylcellulose, Microcrystalline Cellulose ( Microcrystalline Cellulose), Potassium Cellulos e Succinate), Sodium Cellulose Sulfate, and the like, but are not limited thereto.

When the polysaccharide polymer was added, it was confirmed that the solubility of the polymer sericin in water was improved, and aggregation and precipitation between the polymeric sericin was reduced. In addition, it could be observed that the solubility is further improved when a high temperature of 50° C. or higher is applied. It is not clear why the polysaccharide polymer improves the solubility of polymeric sericin, but when the hydrophobic group of the polymer is adsorbed to the hydrophobic region of the polymeric sericin, the hydrophilic group is an ion, and an electrostatic repulsive force occurs in the sericin molecule or between molecules, causing the unfolding state of sericin ( It is presumed that this is because it promotes the random structure) and prevents aggregation between polymeric sericin molecules.

The concentration of the polysaccharide polymer required for such dissolution is not particularly limited, but the weight ratio of the polysaccharide polymer to the polymeric sericin contained in the composition is preferably 0.3 to 5: 1. In addition, the content of the polysaccharide polymer contained in the total composition is preferably 0.03 to 1% by weight.

On the other hand, the solubility of sericin powder may be increased by the addition of the polyacrylate polymer, but when used with a salt, the polyacrylate polymer may cause instability such as creaming and phase separation of the formulation due to viscosity reduction. When used in a large amount, there is a problem of reducing the feeling of use by causing the stickiness of the formulation.

Another aspect of the present invention is to prepare an aqueous phase by dispersing a polysaccharide polymer and a polymer sericin in purified water; Preparing an oil phase containing oil; And mixing and emulsifying the aqueous phase and the oil phase, wherein the polysaccharide polymer is an oil-in-water (O/W) emulsion cosmetic comprising a polymeric sericin, characterized in that the polymeric sericin, which is water-insoluble at room temperature, is dissolved in purified water. It is to provide a method for preparing the composition.

After dispersing the polymeric sericin in purified water, adding an oily phase containing oil to it, emulsifying with a homogenizer, and observing whether an emulsified emulsion is formed.As a result of experimentation, if an oily phase of the same composition is added to an aqueous phase that does not contain a polysaccharide polymer, it is emulsified. Particles were not generated and the oil film was separated, but when the oil phase was added to an aqueous solution containing a polysaccharide polymer and emulsified, emulsified particles were observed, and an emulsion was formed without separating the oil film. Through this, it was confirmed that the polysaccharide polymer plays a role of dissolving the polymeric sericin, which is water-insoluble at room temperature, in purified water, thereby making it possible to prepare a stable oil-in-water type emulsified cosmetic composition without precipitation and aggregation.

The oil component contained in the oil-in-water cosmetic composition according to the present invention is not particularly limited in its kind as long as it is a component commonly used in the art, such as silicone oil, vegetable oil, animal oil, ester oil, or hydrocarbon oil. Etc. can be used. More specifically, for example, more specifically, hydrocarbon-based oils such as squalane, mineral oil, hydrogenated polydecene, hydrogenated polyisobutene, and C12-15 alkylbenzoate; Caprylic/Capric triglyceride, neopentyl glycol dicaprate, 2-octyldodecyl myristate, isopropyl mysterite, isopropyl palmitate, isocetylethylhexanoate, pentaerythrityl tetraethylhexanoate, Ester oils such as butylene glycol dicaprylate/caprate, hexyl laurate, diisostearyl maleate, cetyl 2-ethylhexanoate, octyldodecanol, and glyceryl triethylhexanoate; Vegetable oils such as olive oil, avocado oil, jojoba oil, macadamia seed oil, grape seed oil, and rice bran oil; Animal oils such as lanolin; And silicone oils such as dimethylpolysiloxane, methylphenylpolysiloxane, decamethylcyclopentasiloxane, methylctrimethicone, diphenylsiloxyphenyltrimethicone, caprylylmethicone, phenyltrimethicone, cyclomethicone, and dimethicone. Any one or more oils selected from the group may be used, but the present invention is not limited thereto.

In addition to the above-described components, the oil phase may include various components commonly used in oil phase components of oil-in-water cosmetic compositions in the art. For example, a silicon oil, a polar oil, a liquid oil such as a non-polar oil, a solid oil, and a semi-solid oil may be mentioned, but are not limited thereto.

In addition, the cosmetic composition according to the present invention includes thickeners, dispersants, powders, moisturizers, powders, alcohols, natural polymers, synthetic polymers conventionally blended in water-in-water-type cosmetics within the scope of not impairing the effects of the present invention in addition to the above-described ingredients. , Sugars, antioxidants, buffers, various extracts, stabilizers, preservatives, pigments or flavorings, and other components are blended in an appropriate amount, and can be prepared according to a conventional method.

The present invention also provides a cosmetic comprising a cosmetic composition comprising a polymeric sericin as described above. The cosmetics are nutrient lotion, softening lotion, pack, softening water, emulsion, makeup base, essence, sunscreen cream, sun oil, suspension, emulsion, paste, gel, lotion, powder, oil, emulsion foundation, foundation, solution, It may be prepared in a formulation such as an ointment for external use, a cream, a foam, a patch, and a spray, but is not limited thereto.

The present invention provides a cosmetic composition prepared by dissolving a polymeric sericin and improving the feeling of use such as spreadability. According to the present invention, when the solubility of the polymer sericin in water is improved by using a polysaccharide polymer, when an oil-in-water (O/W) type emulsion is prepared, it can be seen that the feeling of applying skin, such as spreadability, is improved. This shows that sericin, which is discarded as refining wastewater, can be applied to the cosmetic field as an emulsifying ability and improving feeling of use. Since sericin has an amino acid composition similar to that of the skin, it is a protein derived from a natural product that is skin-friendly and can contribute to realizing not only a surfactant ability but also a moisturizing effect and a high sense of adhesion in cosmetic formulations.

Hereinafter, the present invention will be described in more detail through examples. These examples are only for describing the present invention in more detail, and it will be apparent to those of ordinary skill in the art that the scope of the present invention is not limited by these examples.

Example

In the present invention, experiments and measurements were performed in each step as follows in order to solve the problems as in the prior art and check the function.

1. High molecular weight sericin extraction through high temperature and high pressure treatment

To extract the polymeric sericin, put the cocoon or raw silk washed one to three times with lukewarm water in a high-temperature and high-pressure device (autoclave), add 10 to 100 times the weight of the cocoon or raw silk, close the lid, and seal it at 100℃~ Treated at 150° C. for 15 to 90 minutes. Preferably, the cocoon or raw silk was treated with high temperature and high pressure for 20 to 60 minutes at a bath ratio of 1:15 to 1:20 at 100 to 120°C, and the extract was recovered. The recovered extract was filtered through a sieve having a size of 100 to 200 mesh, cooled with a freeze-drying device, and treated with gamma rays to obtain a polymeric sericin in powder form. The molecular weight of the extracted polymer sericin was confirmed through SDS-PAGE, and the average molecular weight was confirmed to be approximately 80 kDa.

2. Comparison of Solubility of Polymer Sericin and Low Molecular Sericin in Water

In order to observe the solubility of the extracted sericin in water, as shown in Table 1 below, sericin was dissolved in purified water at a concentration of 0.1%, heated, and then the degree of precipitation was visually checked.

In order to compare the extracted polymer sericin, the standard sericin was prepared by dissolving Sericin Bombyx mori (molecular weight ~15 kDa) commercially available from Sigma Aldrich in water at the same concentration.

Room temperature 50 ℃ 80 ℃ Extracted polymeric sericin 0.1%
Insoluble
Insoluble
Some dissolving Standard Sericin 0.1%
Dissolution
Dissolution
Dissolution

As shown in Table 1, it was confirmed that the polymeric sericin was not soluble in water even at high temperatures (partially soluble), and it was observed that the 15 kDa sericin was soluble in water even at room temperature. Therefore, it was confirmed that the high molecular weight sericin had a significantly lower solubility in water compared to the low molecular weight. In addition, in order to investigate the surfactant ability of the polymeric sericin, a polymer (Sodium polyacrylate) and a carbomer were dispersed in purified water, and 0, 0.5, and 1.0% of sericin were added and dissolved, and an oil phase was added to prepare an emulsion. As a result of the experiment, it was confirmed that the oil layer was separated immediately after emulsification of the formulation without sericin, and emulsified particles were observed when 0.5% and 1% sericin were added. Therefore, it was confirmed that the polymer sericin prepared above has a surfactant ability.

3. Small molecule Each containing sericin and polymeric sericin Cosmetic Composition of Feeling of use compare

A cosmetic composition was prepared according to the composition in Table 2 below.

Control:
No sericin added Addition of low-molecular sericin Polymer
Sericin
adding GLYCERYL STEARATE 0.5 0.5 0.5 PEG-100 STEARATE 0.5 0.5 0.5 HYDROGENATED POLYISOBUTENE 3 3 3 MACADAMIA TERNIFOLIA SEED OIL 4 4 4 NEOPENTYL GLYCOL DICAPRATE 2 2 2 DIMETHICONE 0.9 0.9 0.9 CYCLOPENTASILOXANE One One One Purified water To 100 To 100 To 100 DIPROPYLENE GLYCOL 6 6 6 1,2-HEXANEDIOL 2 2 2 TRISODIUM EDTA Appropriate amount Appropriate amount Appropriate amount TROMETHAMINE Appropriate amount Appropriate amount Appropriate amount HYDROGENATED LECITHIN 1.3 1.3 1.3 FATTY ALCOHOL 0.9 0.9 0.9 BEHENIC ACID 0.4 0.4 0.4 Standard sericin 0 0.1 0 Extracted polymer sericin 0 0 0.1 SODIUM POLYACRYLATE 0.04 0.04 0.04 CARBOMER 0.22 0.22 0.22

The skin application effect of the cosmetic composition to which the control group, low-molecular sericin, and high-molecular sericin were applied, respectively, that did not contain sericin was compared. Standard sericin (Sigma Aldrich, Sericin Bombyx mori) having a molecular weight of ~15 kDa was used as the low-molecular sericin, and the extracted sericin was used as the polymer sericin. The evaluation items consisted of three items: closeness, moisture, and texture, and were evaluated and compared with 20 women aged 20 to 30 years old. Each evaluation criterion was to give a higher score for each item as the effect was better. The average value of the scores given by each panel is shown in Table 3 below.

<indicator>

2: very bad

4: not good

6: Moderate

8: Excellent

10: very good

Here, "coherence" is evaluated as a value of 2 to 10, where 2 indicates that the feeling of adhesion is low when applying to the skin, and the appearance is high, and 10 indicates that the feeling of adhesion is very excellent when applying to the skin.

"Moisture" is evaluated as a value of 2 to 10, where 2 indicates a lot of feeling of dryness when applied to the skin, and 10 indicates a lot of feeling of moisture when applied to the skin.

"Film feeling" is evaluated as a value of 2 to 10, where 2 shows less residual feeling without feeling of being coated after being absorbed when applied to the skin, and 10 shows that a coating film is formed after being absorbed when applied to the skin, giving a feeling of film without pulling. .

Evaluation item Control: no sericin Contains small molecule sericin Contains high molecular sericin Gives a sense of closeness when applied to the skin 7.9 8.0 9.2 Moisture feels when applied to the skin 8.8 8.9 9.3 Forms a film when applied to the skin 8.2 8.2 8.7

As shown in Table 3, it was confirmed that the overall satisfaction with respect to the adhesiveness, moisture, and film feel of the formulation was increased when the high molecular weight sericin was included compared to the control group not containing sericin and the case containing low molecular weight sericin.

4. Solubility observation by dissolving polymeric sericin in various aqueous polymer solutions

In order to dissolve the extracted polymer sericin in water, various types of polymers, including polysaccharide polymers, were added to water, and the solubilization was observed with the naked eye. 0.1% of sericin was dispersed in 2% of 1,2-Hexanediol aqueous solution, 0.1% of each polymer was added, and the solubility change was observed at room temperature and heating. As the polysaccharide polymer, Xanthan Gum, Alginic Acid, and Cellulose Gum were used. Including Sodium Polyacrylate and Polyacrylic Acid, polyacrylic polymers known to dissolve sericin, Ammonium Acryloyldimethyltaurate/VP Copolymer, Hydroxyethyl Acrylate/Sodium Acryloyldimethyl Taurate Copolymer were used. Was used. The results of observing the solubility are shown in Table 4 below.

Additive material Room temperature 50℃ 80℃ Water (distilled water) Insoluble Insoluble Some dissolving Xanthan Gum
0.1% Some dissolving Mostly dissolved Dissolved Alginic Acid
0.1% Some dissolving Mostly dissolved Dissolved Cellulose Gum
0.1% Some dissolving Mostly dissolved Dissolved Sodium Polyacrylate
0.1% Some dissolving Mostly dissolved Dissolved Polyacrylic Acid0.1% Some dissolving Mostly dissolved Dissolved Ammonium Acryloyldimethyltaurate/VP Copolymer
0.1% Some dissolving Mostly dissolved Dissolved Hydroxyethyl Acrylate/Sodium Acryloyldimethyl Taurate Copolymer0.1% Some dissolving Mostly dissolved Dissolved

As shown in Table 4, when the polymer was not added, the polymer sericin was not dissolved in water, and when 0.1% polysaccharide polymer was used, polyacrylate-based polymer and Ammonium Acryloyldimethyltaurate/VP Copolymer, Hydroxyethyl Acrylate/Sodium Acryloyldimethyl Taurate Copolymer It was confirmed that the polymeric sericin was dissolved at the same level as when was added.

5. Comparison of application properties when sericin is added to aqueous polymer solution

When polymer sericin was dissolved using a polymer as described above, in order to find out the difference in spreadability of each aqueous solution, the frictional force of the aqueous solution was measured and compared, and the results are shown in Table 5 below. The device used to measure the friction force in this experiment is the PARAm (FPT-F1) Friction/Peel Tester, and after applying 0.03g of the formulation onto the artificial leather using a 200g weight, the stainless steel plate is placed at a rate of 100ml/min. The experiment was conducted by moving 100mm and measuring the frictional force.

Raw material name (% by weight) Xanthan Gum 0.1 0 0 0 0 0 0 Alginic Acid 0 0.1 0 0 0 0 0 Cellulose Gum 0 0 0.1 0 0 0 0 Sodium Polyacrylate 0 0 0 0.1 0 0 0 Polyacrylic Acid 0 0 0 0 0.1 0 0 Ammonium Acryloyldimethyltaurate/VP Copolymer 0 0 0 0 0 0.1 0 Hydroxyethyl Acrylate/Sodium Acryloyldimethyl Taurate Copolymer 0 0 0 0 0 0 0.1 Sericin 0.1 0.1 0.1 0.1 0.1 0.1 0.1 Friction force N _Aver. 0.636 0.703 0.619 0.732 0.728 0.892 0.895

As a result of the experiment, it was confirmed that when sericin was added to the gum polymer aqueous solution, the average value of frictional force was lower than that of the polyacrylate-based polymer and acryloyldimethyltaurate-based polymer aqueous solution. This indicates that the spreadability can be improved smoothly when dissolved with a polysaccharide polymer rather than dissolving sericin with a polyacrylate-based polymer and an acryloyldimethyltaurate-based polymer.

In addition, in order to confirm the preferable content ratio of xanthan gum and sericin, xanthan gum was added to the 0.1% sericin aqueous solution so that the total concentration was 0, 0.03, 0.05, 0.1, 0.2, and 0.5%, and the solubility and friction of each aqueous solution were checked .

Raw material name (% by weight) Aqueous solution 1 Aqueous solution 2 Aqueous solution 3 Aqueous solution 4 Aqueous solution 5 Aqueous solution 5 Xanthan Gum 0 0.03 0.05 0.1 0.2 0.5 Sericin 0.1 0.1 0.1 0.1 0.1 0.1 Friction force N _Aver. 1.818 1.059 0.884 0.636 0.418 0.427

As a result of solubility observation, it was confirmed that when xanthan gum was 30% or more by weight of sericin, dissolution occurred. In addition, as a result of checking the frictional force, it was confirmed that when the xanthan gum was 30% or more of the weight of sericin, sufficient dissolution of sericin was performed to improve spreadability and the frictional force value was remarkably reduced. When the weight ratio of xanthan gum and sericin was less than 0.3:1, sericin was not sufficiently dissolved. Therefore, it was confirmed that the preferable content of xanthan gum is 30% or more of the weight of sericin.

In order to find out the upper limit of the preferable xanthan gum, 1% and 1.5% aqueous solutions having increased xanthan gum content were prepared compared to the above aqueous solutions 1 to 5, and frictional force comparison and sensory evaluation were performed.

Raw material name (% by weight) Aqueous solution 5 Aqueous solution 6 Aqueous solution 7 Xanthan Gum 0.5 1.0 1.5 Sericin 0.1 0.1 0.1 Friction force NAver. 0.427 0.517 0.666

As a result of the frictional force comparison, it was confirmed that when the content of xanthan gum was 1% or more, the frictional force value did not decrease any more, but rather increased. In addition, sensory evaluation at the time of skin application was conducted to confirm whether the increase in frictional force was related to the emotional quality. The evaluation items were sticky and stiff, and 20 women aged 20 to 30 were evaluated and compared. Each evaluation criterion was to give a higher score for each item as the effect was smaller. The average values of the scores given by each panel are shown in Table 8 below.

<indicator>

2: very bad

4: not good

6: Moderate

8: Excellent

10: very good

Here, "sticky" is evaluated as a value of 2 to 10, where 2 indicates that the feeling of stickiness is large when applied to the skin, and 10 indicates that it is not sticky when applied to the skin.

"Stiffness" is evaluated as a value of 2 to 10, where 2 indicates that the feeling of stiffness is large when applied to the skin, and 10 indicates that the skin is applied smoothly without being stiff.

Evaluation item Aqueous solution 5 Aqueous solution 6 Aqueous solution 7 Sticky 8.6 6.3 4.2 Stiffness 9.1 6.1 3.7

As a result of sensory evaluation, it was confirmed that when xanthan gum was 1% or more, that is, when the weight ratio of xanthan gum and sericin was 10:1 or more, stickiness and stiffness increased significantly when applied to the skin. Therefore, it can be inferred that using less than 1% of xanthan gum in the formulation will not degrade the emotional quality of cosmetics.

6. Comparison of application properties of cosmetic compositions prepared by adding polymer and sericin together

To compare the spreadability of the formulation prepared by dissolving sericin with a polysaccharide polymer and the formulation prepared by dissolving sericin with other polymers (polyacrylate polymer and acryloyldimethyltaurate polymer, etc.), the composition shown in Table 9 below. Each of the emulsions was prepared and the frictional force was measured. The manufacturing method is as follows.

Glycerin, 1,2-Hexanediol, Trisodium EDTA, Tromethamine, and Sericin were added to purified water, heated to 75°C, and the polymer was sufficiently dispersed. Separately, a thickener, Carbomer, was dispersed in purified water. As for the oil phase, Caprylic/capric triglyceride, Dimethicone, Cyclopentasiloxane, Glyceryl stearate, PEG-100 stearate, and Fatty alcohol were added and heated to 75℃ to dissolve. The aqueous phase was heated to 75°C, the oil phase was slowly added, mixed for 3 minutes with a homomixer, and Carbomer was added for 5 minutes. It was cooled to 28° C. to complete the preparation.

Raw material name (% by weight) Composition 1 Composition 2 Composition 3 Composition 4 Composition 5 Composition 6 Composition 7 GLYCERYL STEARATE 0.5 0.5 0.5 0.5 0.5 0.5 0.5 PEG-100 STEARATE 0.5 0.5 0.5 0.5 0.5 0.5 0.5 CAPRYLIC/CAPRIC TRIGLYCERIDE 7 7 7 7 7 7 7 DIMETHICONE One One One One One One One CYCLOPENTASILOXANE One One One One One One One Purified water To 100 To 100 To 100 To 100 To 100 To 100 To 100 GLYCERIN 6 6 6 6 6 6 6 1,2-HEXANEDIOL 2 2 2 2 2 2 2 TRISODIUM EDTA Appropriate amount Appropriate amount Appropriate amount Appropriate amount Appropriate amount Appropriate amount Appropriate amount TROMETHAMINE Appropriate amount Appropriate amount Appropriate amount Appropriate amount Appropriate amount Appropriate amount Appropriate amount FATTY ALCOHOL 0.5 0.5 0.5 0.5 0.5 0.5 0.5 CARBOMER 0.2 0.2 0.2 0.2 0.2 0.2 0.2 Sericin 0.3 0.3 0.3 0.3 0.3 0.3 0.3 Xanthan Gum 0.1 0 0 0 0 0 0 Alginic Acid 0 0.1 0 0 0 0 0 Cellulose Gum 0 0 0.1 0 0 0 0 Sodium Polyacrylate 0 0 0 0.1 0 0 0 Polyacrylic Acid 0 0 0 0 0.1 0 0 Ammonium Acryloyldimethyltaurate/
VP Copolymer 0 0 0 0 0 0.1 0 Hydroxyethyl Acrylate/Sodium Acryloyldimethyl Taurate Copolymer 0 0 0 0 0 0 0.1 Friction force N _Aver. 0.314 0.504 0.443 0.652 0.634 0.525 0.660

As a result of the evaluation, as shown in Table 9, when the polymeric sericin was contained in the same amount, the formulation containing Xanthan gum, Alginic acid, and Cellulose gum was Sodium polyacrylate, Polyacrylic Acid, Ammonium acryloyldimethyltaurate/VP copolymer, Hydroxyethyl acrylate/sodium acryloyldimethyl. It was confirmed that the average value of frictional force was significantly lower than that of the formulation containing taurate copolymer. Through this, it was confirmed that the formulation prepared by dissolving sericin using a polysaccharide polymer can have smoother and softer application properties than the formulation prepared by dissolving using a polyacrylate-based polymer and an acryloyldimethyltaurate-based polymer.

7. Sensory evaluation of cosmetic composition prepared by adding polymer and sericin together

Using the prepared compositions 1 to 7, sensory evaluation was conducted to compare the feeling of use when applying the skin of a formulation prepared by dissolving sericin with a polysaccharide polymer and a formulation prepared by dissolving sericin with a polyacrylate-based polymer.

The evaluation items consisted of three items: spreadability, adhesion, and absorption, and were evaluated and compared with 20 women aged 20 to 30 years old. Each evaluation criterion was to give a higher score for each item as the effect was better. The average values of the scores given by each panel are shown in Table 10 below.

<indicator>

2: very bad

4: not good

6: Moderate

8: Excellent

10: very good

Here, "applicability" is evaluated as a value of 2 to 10, where 2 indicates that the feeling of applying is not soft and stiff when applied to the skin, and 10 indicates that the applied feeling is smooth and smooth when applied to the skin.

"Adhesion" is evaluated as a value of 2 to 10, where 2 indicates that the feeling of adhesion is low when applying to the skin, and the appearance is high, and 10 indicates that the feeling of adhesion is very excellent when applying the skin.

The "absorption power" was evaluated as a value of 2 to 10, where 2 indicates slow absorption when applied to the skin, and 10 indicates rapid absorption when applied to the skin.

As shown in Table 10, when the polymeric sericin is each contained in the same amount, the formulation containing Xanthan gum, Alginic acid, and Cellulose gum is Sodium polyacrylate, Polyacrylic acid, Ammonium Acryloyldimethyltaurate/VP Copolymer, Hydroxyethyl Acrylate/Sodium Acryloyldimethyl Taurate. Compared to the formulation containing the copolymer, it was found that overall satisfaction with the application, adhesion, and absorption power was higher when applied to the skin. Through this, it was confirmed that the formulation prepared by dissolving sericin using a polysaccharide polymer had an improved feeling of use compared to the formulation prepared by dissolving using a polyacrylate polymer and an acryloyldimethyltaurate polymer.

A preferred content range of the polysaccharide polymer may include about 0.03 to 1 part by weight of polysaccharide relative to the total composition, and when it exceeds 1 part by weight, deterioration in flowability and reduction in feeling of use due to stickiness may occur.

Claims (10)

A cosmetic composition comprising a polysaccharide polymer and high molecular weight sericin. The cosmetic composition of claim 1, wherein the composition is an oil-in-water (O/W) emulsion formulation. The method of claim 1, wherein the polysaccharide polymer is gum, alginic acid, sodium alginate, calcium alginate, starch, agar carrageenan, cellulose-based polymer, gellan, pectin, dextran, glucan, and glucomannan. ), arabino galactan (ARABINO GALACTAN), purcelleran (FURCELLERAN), pullulan (PULLULAN), glucosamine (GLUCOSAMINE) and gelatin (GELATIN) a cosmetic composition characterized in that at least one selected from the group consisting of. The cosmetic composition of claim 1, wherein the high molecular weight sericin has a molecular weight of 25 kDa to 500 kDa. The cosmetic composition of claim 1, wherein the high molecular weight sericin is a powder form obtained by freeze-drying sericin extracted with hot water from silkworm cocoons or silk silk. The cosmetic composition according to claim 1, wherein the weight ratio of the polysaccharide polymer to the high molecular weight sericin contained in the composition is from 0.3 to less than 10: 1. The cosmetic composition according to claim 1, wherein the content of the polysaccharide polymer contained in the composition is from 0.03% by weight to less than 1% by weight. The cosmetic composition of claim 1, wherein the composition further comprises a thickener. Preparing an aqueous phase by dispersing polysaccharide polymer and high molecular weight sericin in purified water;
Preparing an oil phase containing oil; And
Including the step of emulsifying by mixing the aqueous phase and the oil phase,
The polysaccharide polymer is a method for producing an oil-in-water (O/W) emulsion cosmetic composition comprising high molecular weight sericin, characterized in that water-insoluble high molecular weight sericin is dissolved in the purified water at room temperature. A cosmetic comprising the cosmetic composition according to any one of claims 1 to 8.