KR20210017108A - Composition for skin whitening comprising extract of fermented black rice with improved antioxidant activity as effective component - Google Patents

Abstract

The present invention relates to a skin whitening composition comprising, as an active ingredient, a fermented black rice extract with enhanced antioxidant activity. The fermented black rice extract of the present invention is a natural-derived material, is safe for the human body, and has no side effects even when used for a long period of time, and thus can be usefully used as functional cosmetics for skin whitening, and food or a therapeutic agent capable of preventing or ameliorating skin pigmentation diseases.

Description

Composition for skin whitening comprising extract of fermented black rice with improved antioxidant activity as effective component}

The present invention relates to a composition for skin whitening comprising a fermented black rice extract with enhanced antioxidant activity as an active ingredient.

Human skin color is determined by the amount of melanin, carotene, and hemoglobin, of which melanin has the greatest effect, and is also affected by skin thickness, reflectivity, and blood flow. The melanin pigment, which is the most important factor in determining skin color, is guided by tyrosinase, an enzyme contained inside melanocytes, which is a kind of amino acid normally present in the human body. It is converted to (dopa), and finally, melanin, a dark brown polymer, is produced through a series of complex oxidation processes. Tyrosinase is a metal-containing protein that is distributed in almost all species such as microorganisms, plants, and animals, and contains a pair of copper ions in the active site.The function of tyrosinase is closely related to skin-related diseases. So far, studies on whitening agents have been mainly focused on developing inhibitors of tyrosinase activity. In animals, abnormal melanin synthesis causes abnormal melanin pigmentation, such as vitiligo or pigmentation, which can lead to skin cancer in more severe cases.

Currently known methods for whitening include UV blocking, gene expression suppression, enzymatic activity suppression, pigment reduction and stratum corneum removal. Arbutin, kojic acid, ascorbic acid, and hydroquinone are used as representative single substances that exhibit whitening function, and the search for natural substances for the development of natural-friendly whitening agents is actively underway.

On the other hand, black rice is rich in nutrients such as protein, vegetable fat, fiber, minerals, and vitamins, and various effects on antioxidant activity, prevention and treatment of arteriosclerosis, diabetes prevention, and immune function enhancement have been reported. have. Compared to other cereals, the pigment component of black rice contains a variety of polyphenol compounds including flavonoids, which have been confirmed to have physiological activities such as antioxidant, antibacterial and anticancer properties. Black rice containing a large amount of these polyphenol compounds is expected to be developed as a functional food or functional cosmetic material.

Fermentation is a biological technology with a long history. It uses the enzyme action of microorganisms that are beneficial to the human body such as lactic acid bacteria, fungi, bacillus, yeast, etc. to increase the absorption rate in the body by converting natural high molecular substances in foods into low molecular substances, and polysaccharides, oligosaccharides and saponins. It has been known that physiological activity and functional efficacy are increased by obtaining new fermentation products such as, peptide, phytic acid, or the like or by synergistic action between them. Fungal strains, which are one of the microorganisms that mainly degrade carbohydrates and proteins, determine the quality of taste components such as sugars, amino acids, and organic acids produced by enzymes secreted while proliferating, and various physiology as active ingredients such as polyphenols increase. Shows active action. Fungi having such characteristics include Aspergillus oryzae , Aspergillus kawachii , and Aspergillus niger . Among them, Aspergillus kawachi is a white mutant of black bacterium, and it produces carbohydrates, citric acid, and acid-resistant saccharification enzyme, so it is easy to prepare the country, and it has the advantage of making it safe by changing the pH to acid.

Meanwhile, Korean Patent No. 0611428 discloses a'skin whitening cosmetic composition containing black rice extract as an active ingredient', and Korean Patent Publication No. 2005-0108541 discloses'black bean, black rice and black sesame mixed extract as an active ingredient. A cosmetic composition for skin whitening containing as'is disclosed, but there is no description of a composition for skin whitening containing the fermented black rice extract with enhanced antioxidant activity of the present invention as an active ingredient.

The present invention was derived from the above requirements, and the present inventors prepared a fermented black rice extract by inoculating and fermenting black rice powder with Aspergillus kawachii , followed by hot water extraction, and the prepared fermented black rice extract Compared to the non-fermented black rice extract, the total polyphenol and flavonoid content was increased, the DPPH radical scavenging activity was excellent, and the tyrosinase inhibitory activity was significantly increased, thereby completing the present invention.

In order to solve the above problems, the present invention provides a cosmetic composition for skin whitening comprising a fermented black rice extract with enhanced antioxidant activity as an active ingredient.

In addition, the present invention provides a health functional food composition for preventing or improving melanin hyperpigmentation disease comprising a fermented black rice extract as an active ingredient.

In addition, the present invention provides a pharmaceutical composition for the prevention or treatment of melanin hyperpigmentation disease comprising a fermented black rice extract as an active ingredient.

In addition, the present invention provides an antioxidant health functional food composition comprising a fermented black rice extract as an active ingredient.

The fermented black rice extract of the present invention is a natural substance that is safe for the human body and has no side effects even when used for a long period of time, so it can be usefully used as a functional cosmetic for skin whitening, a food or a therapeutic agent capable of preventing or improving skin pigmentation disorders. have.

1 is a result of measuring the total phenolic compound content in non-fermented black rice extract (NF) and fermented black rice extract (F/3).
2 is a result of measuring the flavonoid content in non-fermented black rice extract (NF) and fermented black rice extract (F/3).
3 is a result of measuring the DPPH radical scavenging activity of non-fermented black rice extract (NF) and fermented black rice extract (F/3). The positive control was treated with BHT (butylated hydroxytoluene 0.05%).
4 is a result of measuring tyrosinase inhibition activity of non-fermented black rice extract (NF) and fermented black rice extract (F/3). The positive control group was treated with Arbutin (0.05%).

In order to achieve the object of the present invention, the present invention provides a cosmetic composition for skin whitening comprising a fermented black rice extract with enhanced antioxidant activity as an active ingredient.

In the cosmetic composition for skin whitening according to an embodiment of the present invention, the fermented black rice may be fermented by inoculating fungal bacteria in black rice powder, preferably inoculating Aspergillus kawachii on black rice powder And it may be fermented for 2 to 4 days, preferably for 3 days, but is not limited thereto.

The fermented black rice extract may be extracted using water, a lower alcohol having 1 to 4 carbon atoms, or a mixture thereof as an extraction solvent, and preferably, may be extracted using water as an extraction solvent, but is not limited thereto.

The fermented black rice extract according to the present invention has a higher content of total polyphenols and flavonoids compared to the non-fermented black rice extract and has excellent DPPH radical scavenging activity, as well as inhibiting the tyrosinase activity involved in melanogenesis, thereby inhibiting the antioxidant activity. And skin whitening improvement effect is excellent.

The cosmetic composition for skin whitening of the present invention includes skin, skin softener, skin toner, lotion, milk lotion, moisture lotion, nutrition lotion, massage cream, nutrition cream, eye cream, moisture cream, hand cream, essence, nutrition essence, A pack, cleansing foam, cleansing water, cleansing cream, body lotion, body cleanser, soap, and powder may have any one formulation selected from, but is not limited thereto. The cosmetic composition composed of each of these formulations may contain various bases and additives necessary and appropriate for the formulation of the formulation, and the types and amounts of these components can be easily selected by those skilled in the art.

When the formulation of the cosmetic composition of the present invention is a paste, cream or gel, animal fibers, plant fibers, wax, paraffin, starch, tragacanth, cellulose derivatives, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide as carrier components Etc. may be used.

When the formulation of the cosmetic composition of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In particular, in the case of a spray, additionally chlorofluorohydro Propellants such as carbon, propane-butane or dimethyl ether.

When the formulation of the cosmetic composition of the present invention is a solution or emulsion, a solvent, a solvating agent or an emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene Glycol, 1,3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan.

When the formulation of the cosmetic composition of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, and the like may be used.

When the formulation of the cosmetic composition of the present invention is a surfactant containing cleansing, as carrier components, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, acetionate, imidazolinium derivative, methyltaurate, sarcosinate , Fatty acid amide ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty diethanolamide, vegetable oil, linoline derivative or ethoxylated glycerol fatty acid ester, and the like may be used.

The cosmetic composition of the present invention may further contain excipients including fluorescent substances, fungicides, hydrophobic substances, moisturizers, fragrances, fragrance carriers, proteins, solubilizers, sugar derivatives, sunscreen agents, vitamins, plant extracts, etc. .

In addition, the present invention provides a health functional food composition for preventing or improving melanin hyperpigmentation disease comprising a fermented black rice extract as an active ingredient.

The health functional food composition of the present invention has a higher content of total polyphenols and flavonoids compared to the non-fermented black rice extract, and has increased DPPH radical scavenging activity and inhibitory activity of tyrosinase involved in melanin production. As an active ingredient, it is effective against melanin hyperpigmentation diseases.

In the present invention, the term "hyperpigmentation" means that a specific area of the skin or nails or nails becomes blacker or darker than other areas due to an excessive increase in melanin. The melanin hyperpigmentation disorders include freckles, senile spots, liver spots, melasma, brown or dark spots, solar pigment spots, cyanicmelasma, hyperpigmentation after drug use, gravidic chloasma, or scratches and burns. It may be hyperpigmentation after inflammation due to wounds or dermatitis including, but is not limited thereto.

The health functional food composition for preventing or improving the melanin hyperpigmentation disease may be prepared in any one formulation selected from powders, granules, pills, tablets, capsules, candy, syrup, effervescent tablets, and beverages, but is not limited thereto. . The health functional food composition is not particularly limited as long as it can be ingested to prevent or improve melanin hyperpigmentation disease.

When the fermented black rice extract of the present invention is used as a food additive, the fermented black rice extract may be added as it is or used with other foods or food ingredients, and may be appropriately used according to a conventional method. The active ingredient may be appropriately used depending on the purpose of use (prevention or improvement). In general, in the manufacture of food or beverage, the fermented black rice extract of the present invention is added in an amount of 15 parts by weight or less, preferably 10 parts by weight or less based on the raw material. However, in the case of long-term intake for the purpose of health control, it can be used in an amount within a range without any problems in terms of safety.

There is no particular limitation on the type of food. Examples of foods to which the health functional food composition can be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum, dairy products including ice cream, various soups, beverages, tea There are drinks, alcoholic beverages, and vitamin complexes, and all health foods in the usual sense are included. The beverage includes carbonated beverages, functional ionic beverages, juices (eg, apples, pears, grapes, aloe, tangerines, peaches, carrots, tomato juice, etc.), sikhye, and the like.

Functional foods of the present invention include ingredients that are commonly added during food production, and include, for example, proteins, carbohydrates, fats, nutrients, and seasonings. For example, when prepared as a drink, natural carbohydrates or flavoring agents may be included as additional ingredients in addition to the active ingredient. The natural carbohydrates are monosaccharides (eg, glucose, fructose, etc.), disaccharides (eg, maltose, sucrose, etc.), oligosaccharides, polysaccharides (eg, dextrins, cyclodextrins, etc.) or sugar alcohols (eg , Xylitol, sorbitol, erythritol, etc.). The flavoring agent may be a natural flavoring agent (eg, taumatin, stevia extract, etc.) and a synthetic flavoring agent (eg, saccharin, aspartame, etc.). In addition to the health functional food composition, various nutrients, vitamins, electrolytes, flavoring agents, coloring agents, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonic acid It may further contain a carbonation agent used in beverages.

In addition to the above health functional food composition, various nutrients, vitamins, inhibitors, flavoring agents, coloring agents, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, It may further contain a carbonating agent used in carbonated beverages. Although the ratio of the ingredients to be added is not very important, it is generally selected from 0.01 to 0.1 parts by weight based on 100 parts by weight of the health functional food composition of the present invention.

In addition, the present invention provides a pharmaceutical composition for the prevention or treatment of melanin hyperpigmentation disease comprising a fermented black rice extract as an active ingredient.

The melanin hyperpigmentation disorders include freckles, senile spots, liver spots, melasma, brown or dark spots, solar pigment spots, cyanicmelasma, hyperpigmentation after drug use, gravidic chloasma, or scratches and burns. It may be hyperpigmentation after inflammation due to wounds or dermatitis including, but is not limited thereto.

The pharmaceutical composition of the present invention is effective in fermented black rice extract with increased total polyphenol and flavonoid content compared to non-fermented black rice extract and increased DPPH radical scavenging activity and inhibitory activity of tyrosinase involved in melanogenesis. It is included as an ingredient, and is effective against melanin hyperpigmentation diseases.

The pharmaceutical composition comprising the fermented black rice extract of the present invention may further include a suitable carrier, excipient, or diluent commonly used in the manufacture of pharmaceutical compositions.

The pharmaceutical dosage form of the pharmaceutical composition of the present invention may be used alone or in combination with other pharmaceutically active compounds, as well as in an appropriate combination.

The pharmaceutical composition according to the present invention may be formulated and used in the form of oral preparations such as powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, etc., external preparations, suppositories, and injections according to a conventional method. . Carriers, excipients and diluents that may be included in the pharmaceutical composition include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, gum acacia, alginate, gelatin, calcium phosphate, calcium silicate, cellulose , Methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, and mineral oils. In the case of formulation, it is prepared using diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrants, and surfactants that are usually used. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, and these solid preparations contain at least one excipient, such as starch, calcium carbonate, sucrose or lactose, gelatin, etc. in the fermented product. It is prepared by mixing. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Liquid preparations for oral use include suspensions, liquid solutions, emulsions, syrups, and other excipients, such as water and liquid paraffin, which are commonly used simple diluents, such as wetting agents, sweeteners, fragrances, and preservatives. have. Preparations for parenteral administration include sterile aqueous solutions, non-aqueous solutions, suspensions, emulsions, lyophilized preparations, and suppositories. Propylene glycol, polyethylene glycol, vegetable oils such as olive oil, injectable esters such as ethyl oleate, etc. may be used as the non-aqueous solvent and suspension. As a base for suppositories, Witepsol, Macrogol, Tween 61, cacao butter, laurin paper, glycerogelatin, and the like can be used.

The preferred dosage of the pharmaceutical composition of the present invention varies depending on the condition and weight of the patient, the degree of disease, the form of the drug, the route and duration of administration, but may be appropriately selected by those skilled in the art. The pharmaceutical composition of the present invention can be administered to mammals such as mice, mice, livestock, and humans by various routes. All modes of administration can be expected and may be administered by, for example, oral, rectal or intravenous, intramuscular, subcutaneous, intrauterine dura mater or intracerebroventricular injection.

In addition, the present invention provides an antioxidant health functional food composition comprising a fermented black rice extract as an active ingredient. The fermented black rice extract according to the present invention has a higher content of total polyphenols and flavonoids compared to the non-fermented black rice extract and has excellent DPPH radical scavenging activity, so it can be used as a health functional food for antioxidants.

Hereinafter, the present invention will be described in detail by examples. However, the following examples are only illustrative of the present invention, and the contents of the present invention are not limited to the following examples.

Materials and methods

1. Experimental materials

Black rice was provided by OK Bio Lab Co., Ltd., and the fermentation strain was selected and used as a fungus, Aspergillus kawachii KCCM 32819 strain, and pre-cultured in YM liquid medium (Yeast Malt broth, Difco). KCCM 32819 strain (1.0 × 10 ⁴ to 1.0×10 ⁶ CFU/mL) was inoculated into the black rice powder at a ratio of 1:1 by weight, fermented at 37°C for 3 days, and dried with hot air at 60°C for 8 hours to powder.

2. Conditions for extraction of fermented black rice

The aqueous extract was refluxed three times at 37°C for 3 hours after adding 10 times of distilled water to 100 g of unfermented black rice powder and dry powder of fermented black rice, and collected each extract with Adventec 110 mm No.2 filter paper ( Toyo 2A; Toyo Roshi, Japan) was filtered and used in the following examples. The experimental group was performed by setting the non-fermented black rice extract (NF) and the fermented black rice extract (F/3).

3. Measurement of phenolic compound content

The content of phenolic compounds was measured by the Folin-Denis method using the principle that a phenolic substance reacts with phosphormolybdic acid to give a blue color. To 0.5 ml of a 0.1% (w/v) sample solution, 2.5 ml of a Folin-ciocalteu phenol solution was added, mixed, and allowed to stand at room temperature for 5 minutes. After reacting for exactly 5 minutes, 2 ml of 7.5% sodium carbonate (Na ₂ CO ₃ ) was added and mixed, followed by color development at 50°C for 5 minutes, and then 760 nm using a spectrophotometer (Hitachi U-2900, Hitachi High-Technologies Co., Japan). Absorbance was measured at. At this time, the content of phenolic compounds was calculated from a standard curve prepared by measuring tannic acid at a concentration of 0 to 500 ㎍/㎖ in the same manner as in the sample.

4. Flavonoid content measurement

To measure the flavonoid content, 1.25 ml of purified water and 5 ml of 5% sodium nitrite (NaNO ₂ ) solution were added to 0.25 ml of 0.1% (w/v) sample solution. After 5 minutes, 10% aluminum chloride hexahydrate ( 0.15 ml of AlCl ₃ ·6H ₂ O) was mixed, and the absorbance of the mixture was measured at 510 nm using a spectrophotometer. At this time, the flavonoid content was calculated from a standard curve prepared by measuring (+)-catechin hydrate as a standard substance at a concentration of 20 to 200 μg/ml in the same manner as the sample.

5. Measurement of antioxidant activity by DPPH

To measure the antioxidant activity, 16 mg of DPPH (α,α'-diphenyl-β-picrylhydrazyl) was dissolved in 100 ml of ethanol, and 100 ml of distilled water was mixed. Whatman filter paper NO. Filtered through 2 to prepare a DPPH reaction solution. Make 1 ml of non-fermented black rice extract or fermented black rice extract to a concentration of 10% (w/v), add 5 ml of DPPH reaction solution, mix, react in the dark for 30 minutes, and measure the absorbance at 528 nm using a spectrophotometer. Measured. At this time, for activity comparison, absorbance was measured in the same manner as the sample using BHT (butylated hydroxytoluene), a synthetic antioxidant that has been widely used as an antioxidant, at a concentration of 0.05%. Antioxidant activity using DPPH was expressed as a percentage (%) of the difference in absorbance between the sample added and the non-added group. The equation for obtaining the DPPH radical scavenging activity is as follows.

DPPH radical scavenging activity (%)=[1-(sample absorbance _528nm )/control absorbance _528nm ]×100

6. Tyrosinase ( tyrosinase ) Activity measurement

To measure the in vitro mushroom tyrosinase activity inhibitory activity, 2.5mM L-DOPA (3,4-dihydroxyphenylalanine) 0.3ml, 1% (w/v) concentration in 1.5ml plastic cuvette. After mixing 0.05 ml of non-fermented black rice extract or fermented black rice extract and 0.1 M phosphate buffer solution (pH 6.8) and reacting at 25°C, 1,380 units/ml mushroom tyrosinase (2,500 units, Sigma Chemical Co., St. USA) 0.05 ml was added and the absorbance was measured and calculated at 470 nm using a spectrophotometer while reacting at 25° C. for 2 minutes. A sample dissolution solution containing no sample was used as a control, and 0.05% arbutin, known as a tyrosinase inhibitor, was used as a positive control. The formula for obtaining the tyrosinase inhibitory activity is as follows.

Tyrosinase activity inhibition rate (%)=100-[(A-B)/A] X 100

(A; difference in absorbance between 0.5 and 1 minute of the reaction solution without sample, B; difference in absorbance between 0.5 and 1 minute of the reaction solution containing sample)

7. Statistical processing

The results obtained from the above experiment were expressed as the mean value and standard deviation (mean±SE) by the one-way ANOVA test, and the verification of significance between each experimental group was indicated by Duncan's multiple range test.

Example 1. Analysis of Total Polyphenol and Flavonoid Contents of Fermented Black Rice Extract

Polyphenol compounds are secondary metabolites that are widely distributed in the plant world and are largely classified into flavonoids, catechins, and tannins. In particular, phenolic compounds are known to exhibit high antioxidant activity due to their electron donating ability.

As a result of measuring the total polyphenol and flavonoid contents of the non-fermented black rice extract and the fermented black rice extract, the total polyphenol content was 14.59 mg/100 g in the non-fermented black rice extract treatment group (NF), and the fermented black rice extract treatment group (F/3). Was 42.28mg/100g (Fig. 1), and the flavonoid content was 18.23mg/100g in the NF group and 50.67mg/100g in the F/3 group (FIG. 2). That is, total polyphenol content and flavonoid content were increased in fermented black rice extract compared to non-fermented black rice extract.

Example 2. Fermented Black Rice Extract DPPH Radical Scavenging activity assay

The DPPH radical scavenging activity of the non-fermented black rice extract and the fermented black rice extract was measured, and the positive control group was treated with 0.05% of BHT, a commercially available synthetic antioxidant. As a result, DPPH radical scavenging activity was 17.5% in the non-fermented black rice extract treatment group (NF), and the DPPH radical scavenging activity was significantly increased to 53.33% in the fermented black rice extract treatment group (F/3) (FIG. 3).

Example 3. Analysis of Tyrosinase Inhibitory Activity of Fermented Black Rice Extract

Melanin is a pigment component that is widely distributed in living organisms including humans such as skin, hair, and eyes. In the human body, it is synthesized from melanosomes in melanocytes in the epidermis, and is a kind of amino acid, tyrosine. As a starting material, it is oxidized to DOPA (3,4-dihydroxy-phenylalanine) or DOPA quinone by tyrosinase enzyme, followed by a non-enzymatic reaction and an automatic oxidation process. Melanin is synthesized through polymerization. Since the melanin produced at this time darkens the color of the skin, an experiment that effectively inhibits the activity of tyrosinase is evaluated as a very useful research method to confirm the whitening effect. In the positive control group, 0.05% of commercially available arbutin was treated.

As a result of measuring the tyrosinase inhibitory activity of the non-fermented black rice extract and the fermented black rice extract, as disclosed in FIG. 4, no tyrosinase inhibitory activity was observed in the non-fermented black rice extract treatment group (NF), but the fermented black rice extract treatment In group (F/3), it was remarkably increased to about 21%, which is a synergistic synergistic effect of Aspergillus kawachii , a fungus used for black rice and fermentation, and it is believed that the active ingredient of whitening activity is released . Became.

Claims (7)

A cosmetic composition for skin whitening comprising a fermented black rice extract with enhanced antioxidant activity as an active ingredient. The cosmetic composition for skin whitening according to claim 1, wherein the fermented black rice is fermented by inoculating black rice powder with Aspergillus kawachii . A health functional food composition for preventing or improving melanin hyperpigmentation disease comprising fermented black rice extract as an active ingredient. The method of claim 3, wherein the composition is powder, granule, pill, tablet, capsule, candy, syrup and
A health functional food composition for preventing or improving melanin hyperpigmentation disease, characterized in that it is prepared in any one formulation selected from beverages. A pharmaceutical composition for preventing or treating melanin hyperpigmentation disease comprising fermented black rice extract as an active ingredient. The method of claim 5, wherein the melanin hyperpigmentation disease is freckles, senile spots, liver spots, melasma, brown or black spots, sun pigment spots, cyanic melasma, hyperpigmentation after drug use, gestational brown spots (gravidic chloasma), or a pharmaceutical composition for the prevention or treatment of hyperpigmentation disorders of melanin, characterized in that hyperpigmentation after inflammation due to wounds or dermatitis. Antioxidant health functional food composition comprising fermented black rice extract as an active ingredient.

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