KR102515649B1 - Cosmetic composition for skin whitening and anti-wrinkle comprising extract of steamed Polygonatum sibiricum as effective componenet - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising an extract of sagebrush as an active ingredient. Since it is excellent, it can be usefully used as a cosmetic or health functional food for skin whitening and wrinkle improvement, or as a material for the treatment of melanin pigmentation disease.

Description

Cosmetic composition for skin whitening and anti-wrinkle comprising extract of steamed Polygonatum sibiricum as effective componenet}

The present invention relates to a cosmetic composition comprising an extract of St. John's wort as an active ingredient.

Skin aging refers to both tangible and intangible changes that appear in the skin as we age. Skin aging can be divided into endogenous physiological aging, which is naturally induced due to a decrease in physiological functions of a living body as age increases, and extrinsic photoaging, which is progressed by long-term exposure to ultraviolet rays. The clinical features of endogenous skin aging are skin thinning and loss of elasticity. In addition, as histological findings, the thickness of the epidermis and dermis is thinned, blood vessels are reduced, and the number of fibroblasts in the dermis is reduced. In addition, the clinical features of photoaging, which is extrinsic skin aging, are that the skin becomes rough and loses its elasticity, irregular pigmentation occurs, and deep wrinkles increase.

Melanin is a major factor determining the color of hair and skin. It serves to protect skin cells from ultraviolet rays, but accumulation of excessive melanin causes excessive pigmentation such as freckles or melasma. Melanin-forming cells present in the basal layer of the epidermal layer have specific structures called melanosomes, where melanin is produced due to the action of melanogenic enzymes. Melanin consists of black and brown eumelanin and yellow and red pheomelanin. It starts with conversion to quinone (DOPA quinone).

In addition, various causes of wrinkles have been reported. First, when the skin is exposed to excessive ultraviolet rays, the decomposition of collagen, which is a skin component, is promoted, and the skin loses elasticity and wrinkles may be generated. In addition, when the skin becomes excessively dry due to excessive temperature changes, low humidity, or wind, the function of the skin as a barrier against the outside is deteriorated, resulting in wrinkles. In addition, when the skin is exposed to reactive oxygen species or free radicals, lipid peroxide is produced by oxidation, and as a result, skin constituent proteins such as collagen are modified to form wrinkles. Various cosmetic compositions are being studied to suppress the occurrence of wrinkles caused by these external and internal factors. In particular, in the case of functional cosmetics containing chemical components, there is a problem that causes skin irritation and is not suitable for sensitive skin, so attention is focused on functional cosmetics derived from natural plants that are less irritating to the skin and are eco-friendly.

Polygonatum sibiricum Redoute is a perennial herb belonging to the genus Polygonatum in the Liliaceae family. there is. The origin plant of Hwangjeong is not only the layered hooked gullet, but also steamed rhizomes of Polygonatum kingianum Coll. et Hemsley, Polygonatum falcatum A. Gray, and Polygonatum cyrtonema Hua. can be used There are 18 species of plants reported in Korea as plants of the genus Polygonatum, including Polygonatum odoratum Druce var. It is known that the rhizome and young leaves of the rhizome and young leaves are used for food in spring, and in oriental medicine, the rhizome is used as a nourishment and weakness after illness.

In the present invention, the physiological activity changes according to the temperature and time of the steaming process of the leaves and rhizomes of the steaming process were confirmed. The applicability of the extracts from the leaves and rhizomes of Brachius japonica as a cosmetic functional material was evaluated.

On the other hand, Korean Patent Publication No. 2018-0060607 discloses a 'composition for improving skin' containing as an active ingredient a mixed extract obtained by mixing goji berry extract and hwangjeong extract that have not been steamed, and Korean Patent No. 1018531 discloses a 'composition for improving skin'. A cosmetic composition for skin whitening and wrinkle improvement containing the extract of the present invention as an active ingredient, although a 'composition for preventing and treating obesity or metabolic syndrome containing an extract of A. There is nothing written about it.

The present invention has been derived from the above needs, and the present inventors have steamed the leaves or rhizomes of St. John's wort under various temperature and time conditions, and then the DPPH and ABTS radical scavenging activity and the total phenolic and flavonoid content enhancing effects are the most Excellent steaming conditions were selected. As a result of measuring the collagenase and tyrosinase inhibitory activity using the extract of the leaf or rhizome prepared under the steaming conditions, the leaves or rhizomes of the non-steamed The present invention was completed by confirming that the collagenase and tyrosinase inhibitory effects were excellent in the group treated with the extract of the leaves or rhizomes of the steamed rhizome, compared to the group treated with the extract of.

In order to solve the above problems, the present invention provides a cosmetic composition for skin whitening and wrinkle improvement comprising an extract of sagebrush as an active ingredient.

In addition, the present invention provides a health functional food composition for skin whitening and wrinkle improvement comprising an extract of sagebrush as an active ingredient.

In addition, the present invention provides a pharmaceutical composition for the prevention or treatment of hypermelanin hyperpigmentation disease, comprising an extract of sagebrush as an active ingredient.

Since the steamed extract of the leaf or rhizome of the present invention has excellent collagenase and tyrosinase inhibitory activities, it is used as a cosmetic or health functional food for skin whitening and wrinkle improvement, or for the treatment of melanin pigmentation disease. It is expected to be useful as a material.

In order to achieve the object of the present invention, the present invention provides a cosmetic composition for skin whitening and wrinkle improvement comprising an extract of St. John's wort as an active ingredient.

In the cosmetic composition of the present invention, the extract of the layered hooked bridle may be prepared by ultrasonic extraction after steaming the leaves or rhizomes of the layered hooked bridle, preferably using the leaves or rhizomes of the layered hooked bridle. It may be prepared by ultrasonic extraction after steaming at 110 ~ 130 ℃ for 10 ~ 14 hours, most preferably prepared by ultrasonic extraction after steaming the leaves or rhizomes at 120 ℃ for 12 hours. It may, but is not limited thereto.

In the cosmetic composition of the present invention, the extract of the leaves or rhizomes steamed at 110 to 130 ° C. for 10 to 14 hours is treated under different temperature and time conditions from the extract of the leaves or rhizomes that are not steamed. It has the most excellent antioxidant activity compared to the extracts of the leaves or rhizomes steamed with

In addition, the extract of the leaf or rhizome of the leaf or rhizome with excellent antioxidant activity prepared by steaming at 110 to 130 ° C. for 10 to 14 hours inhibited tyrosinase compared to the leaf or rhizome extract of the non-steamed leaf or rhizome. It is effective in skin whitening due to its excellent activity, and is effective in improving skin wrinkles due to its excellent collagenase inhibitory activity.

The cosmetic composition of the present invention is a cream, softening lotion, nutrient lotion, pack, essence, hair tonic, shampoo, rinse, hair conditioner, hair treatment, gel, skin lotion, skin softener, skin toner, astringent, milk lotion, moisture lotion , Nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, foundation, nutrition essence, sunscreen, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion, and body cleanser. may have, but is not limited thereto. The cosmetic composition composed of each of these formulations may contain various bases and additives necessary and appropriate for formulation of the formulation, and the types and amounts of these components can be easily selected by those skilled in the art.

When the formulation of the cosmetic composition of the present invention is a paste, cream or gel, animal fiber, vegetable fiber, wax, paraffin, starch, tragacanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide as a carrier component etc. can be used.

When the formulation of the cosmetic composition of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluorohydro propellants such as carbon, propane-butane or dimethyl ether.

When the formulation of the cosmetic composition of the present invention is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene fatty acid esters of glycol, 1,3-butylglycol oil, glycerol aliphatic esters, polyethylene glycol or sorbitan.

When the formulation of the cosmetic composition of the present invention is a suspension, a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracanth and the like may be used.

When the formulation of the cosmetic composition of the present invention is surfactant-containing cleansing, as carrier components, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, acethionate, imidazolinium derivative, methyl taurate, and sarcosinate , fatty acid amide ether sulfates, alkylamidobetaines, fatty alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives, or ethoxylated glycerol fatty acid esters.

The cosmetic composition of the present invention may further contain excipients including fungicides, hydrotropes, humectants, fragrances, fragrance carriers, proteins, solubilizers, sugar derivatives, sunscreens, vitamins, plant extracts, and the like.

The present invention also provides a health functional food composition for skin whitening and wrinkle improvement comprising an extract of a layered barnacle as an active ingredient.

In the health functional food composition of the present invention, the layered barnacles extract is as described above.

As used herein, the term 'whitening' refers to inhibiting, inhibiting, or alleviating hyperpigmentation of the skin. Hyperpigmentation of the skin includes freckles, melasma, hyperpigmentation after ultraviolet exposure, hyperpigmentation after inflammation, senile lentigines, brown spots or age spots.

The health functional food composition of the present invention contains, as an active ingredient, the steamed extract of the leaf or rhizome of the present invention, which has excellent tyrosinase and collagenase inhibitory activity, and is effective in skin whitening and wrinkle improvement. .

The health functional food composition of the present invention has the advantage of having a more excellent effect by ingesting it in the form of inner beauty food. The inner beauty refers to food referred to as 'eating cosmetics or beauty food', which absorbs various ingredients good for the skin into the body and changes the skin constitution to a healthy one. You can select and consume the inner beauty food that suits your individual lifestyle considering your lifestyle. When cosmetics containing the cosmetic composition described above and the inner beauty food containing the extract of the present invention are mixed, the skin whitening and wrinkle improvement effects are significantly increased compared to using only cosmetics, resulting in more effective skin whitening and wrinkle improvement. It has the advantage of being effective.

When using the extract of the present invention as a food additive, the extract of the present invention may be added as it is or used together with other foods or food ingredients, and may be appropriately used according to a conventional method. Mixed amounts of active ingredients can be appropriately used depending on the purpose of use (prevention or improvement). In general, when preparing food or beverage, the health functional food composition of the present invention is added in an amount of 15 parts by weight or less, preferably 10 parts by weight or less, based on the raw material. However, in the case of long-term intake for health purposes, the amount may be less than the above range.

There is no particular limitation on the type of food. Examples of foods to which the health functional food composition can be added include meat, sausages, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum, dairy products including ice cream, various soups, beverages, tea There are drinks, alcoholic beverages, and vitamin complexes, and includes all health foods in a conventional sense. The beverage includes carbonated beverages, functional ionic beverages, juice (eg, apple, pear, grape, aloe, tangerine, peach, carrot, tomato juice, etc.), sikhye, and the like.

The functional food of the present invention includes components commonly added during food preparation, and includes, for example, proteins, carbohydrates, fats, nutrients, and seasonings. For example, when prepared as a drink, natural carbohydrates or flavors may be included as additional ingredients in addition to active ingredients. The natural carbohydrates are monosaccharides (eg, glucose, fructose, etc.), disaccharides (eg, maltose, sucrose, etc.), oligosaccharides, polysaccharides (eg, dextrins, cyclodextrins, etc.) or sugar alcohols (eg, maltose, sucrose, etc.) , xylitol, sorbitol, erythritol, etc.) are preferred. As the flavoring agent, natural flavoring agents (eg, thaumatin, stevia extract, etc.) and synthetic flavoring agents (eg, saccharin, aspartame, etc.) may be used. In addition to the health functional food composition, various nutrients, vitamins, electrolytes, flavors, colorants, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonated beverages It may further contain a carbonation agent used for

In addition to the health functional food composition, various nutrients, vitamins, inhibitors, flavoring agents, colorants, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, It may further contain a carbonating agent used in carbonated beverages. The ratio of the components to be added is not very important, but is generally selected in the range of 0.01 to 0.1 parts by weight based on 100 parts by weight of the health functional food composition of the present invention.

The present invention also provides a pharmaceutical composition for the prevention or treatment of hypermelanin hyperpigmentation disease, comprising an extract of sagebrush as an active ingredient.

In the pharmaceutical composition of the present invention, the extract of the lamellar chinensis is as described above.

The pharmaceutical composition of the present invention contains as an active ingredient the steamed extract of the leaf or rhizome of the present invention, which has excellent tyrosinase inhibitory activity, and is effective in improving skin whitening.

In the present specification, the term “melanin hyperpigmentation” means that certain parts of the skin or nails or nails become black or dark compared to other parts due to an excessive increase in melanin. The melanin hyperpigmentation disease is freckles, senile spots, liver spots, melasma, brown or sunspots, solar pigment spots, cyanic melasma, hyperpigmentation after drug use, pregnancy brown spots (gravidic chloasma), or scratches and hyperpigmentation after wounds including burns or inflammation due to dermatitis; and the like, but are not limited thereto.

The pharmaceutical composition of the present invention may include pharmaceutically acceptable carriers in addition to active ingredients, and these carriers are commonly used in preparation, and include lactose, dextrose, sucrose, sorbitol, mannitol, starch, gum acacia, Calcium phosphate, alginate, gelatin, calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, water, syrup, methyl cellulose, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and minerals. oils and the like, but are not limited thereto. The pharmaceutical composition of the present invention may further include a lubricant, a wetting agent, a sweetening agent, a flavoring agent, an emulsifying agent, a suspending agent, a preservative, and the like in addition to the above components. When adding the above components, skin safety according to combined use, ease of formulation, and stability of active ingredients may be considered.

The pharmaceutical composition of the present invention can be administered orally or parenterally, and in the case of parenteral administration, it can be administered by topical application to the skin, intravenous injection, subcutaneous injection, intramuscular injection, intraperitoneal injection, transdermal administration, and the like. Considering that the pharmaceutical composition of the present invention is applied for the treatment or prevention of melanin hyperpigmentation disease, it is preferably applied topically to the skin.

The pharmaceutical composition of the present invention is prepared in unit dosage form by formulating it using a pharmaceutically acceptable carrier or excipient according to a method that can be easily performed by those skilled in the art, or It can be prepared by incorporating into a dose container. At this time, the formulation may be prepared as any one formulation selected from injections, creams, patches, sprays, ointments, warnings, lotions, liniments, pastas, and cataplasmas, and may additionally include a dispersing agent or a stabilizer. there is.

The pharmaceutical composition of the present invention can provide a desirable effect of improving skin whitening when it contains an effective amount of the extract of St. John's wort. In the present invention, the term 'effective amount' refers to the amount of the extract of Porphyra japonica that can exhibit the effect of suppressing pigmentation. The effective amount of the extract contained in the composition of the present invention will vary depending on the form in which the composition is commercialized, how the extract is applied to the skin, and how long it stays on the skin. The preferred dosage of the pharmaceutical composition containing the extract of the present invention as an active ingredient varies depending on the condition and body weight of the patient, the severity of the disease, the type of drug, the route and duration of administration, but can be appropriately selected by those skilled in the art. .

Hereinafter, the present invention will be described in detail by examples. However, the following examples are only to illustrate the present invention, and the content of the present invention is not limited to the following examples.

Materials and Methods

1. Steaming and sampling

Polygonatum sibiricum Redoute leaves and rhizomes were collected from Kangwon National University farm and used in the experiment. Washing the leaves and rhizomes of the layered hooked toadstool, and using a wet high-pressure steamer (HK-AC200, Hankuk S&I Co., Korea) at 60 ° C, 80 ° C, 100 ° C or 120 ° C temperature conditions 1, 3, 6, After steaming for 12 or 24 hours, it was dried for 3 days in a dryer (LBI-500E, Labtech Co., Ltd., Korea).

After pulverizing the dried sample with a grinder (FM 909T, Hanil Electric Co., Ltd., Korea), take 30 g of powder sample, add 300 g of purified water, and ultrasonic (Power sonic 505, Hwashin Tech Co., Ltd.) for 1 hour. ., Korea) After extraction, it was filtered with filter paper (No.20, Hyundai micro Co., Ltd., Korea), and the filtrate was concentrated using a rotary vacuum concentrator (Evaporator N-1000, EYELA, Japan) and used as a sample. did

2. DPPH radical scavenging activity

In order to measure the DPPH radical scavenging activity of the extract of P. chinensis, 100 μl of 0.15 mM DPPH (2,2-diphenyl-1-picrylhydrazyl) solution and 100 μl of each concentration were added to a 96-well plate and reacted at room temperature for 30 minutes. Then, absorbance was measured at 517 nm using a UV/Vis spectrophotometer (Versa-Max, Molecular Devices, USA). DPPH radical scavenging activity was expressed as RC ₅₀ (reducing concentration of 50%), and L-ascorbic acid was used as a positive control.

3. ABTS radical scavenging activity

In order to measure the ABTS radical scavenging activity of the extract of sagebrush, 7.4 mM ABTS [2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt] and 2.6 mM potassium persulfate (K ₂ S ₂ O ₈ ) was mixed and reacted for 15 hours in the dark at room temperature (25°C), and then diluted to obtain an absorbance of 0.70±0.05 at 734 nm using a UV/Vis spectrophotometer, and the reaction reagent was used. After adding 90 μl of ABTS solution and 10 μl of each concentration sample to a 96-well plate and reacting at room temperature for 10 minutes, absorbance was measured at 734 nm using a UV/Vis spectrophotometer (Versa-Max). ABTS radical scavenging activity was expressed as RC ₅₀ , and L-ascorbic acid was used as a positive control.

4. Determination of Total Phenol Content

In order to measure the total phenolic content of the extract of St. John's wort, 100 μl of 1 mg/ml sample and 50 μl of Folin &Ciocalteu's phenol reagent were added, reacted at room temperature for 3 to 5 minutes, and then diluted with 300 μl of distilled water to 20 μl. % sodium carbonate (Na ₂ CO ₃ ) was added and reacted for 15 minutes. After the reaction was completed, 1 ml of distilled water was added, centrifuged at room temperature for 2 minutes, and 200 μl of the supernatant was added to a 96-well plate, and absorbance was measured at 740 nm using a UV/Vis spectrophotometer (Versa-Max). A calibration curve was prepared by diluting gallic acid as a standard substance to a concentration of 0.05, 0.10 or 0.20 mg/ml, and expressed as gallic acid equivalent (mg GAE/g).

5. Determination of total flavonoid content

In order to measure the total flavonoid content of the extract of sagebrush, 100 μl of 10% aluminum nitrate and 100 μl of 1 M potassium acetate were mixed and 500 μl of 1 mg/ml sample was added. It was reacted for 40 minutes. After the reaction was completed, 150 μl of the mixture was added to a 96-well plate, and absorbance was measured at 415 nm using a UV-Vis spectrophotometer (Versa-Max). A calibration curve was prepared by diluting quercetin as a standard substance to a concentration of 0.05, 0.10 or 0.20 mg/ml, and expressed as quercetin equivalent (mg QE/g).

6. Collagenase inhibitory activity

In order to measure the collagenase inhibitory effect of the extract of P. japonica, 4 mM CaCl ₂ and 4-phenylazobenzyloxy carbonyl-Pro-Leu-Gly-Pro-D-Arg in 0.1 M Tris-HCl buffer (pH 7.5). (0.3 mg/ml) was mixed with 250 μl of substrate solution, 100 μl of sample, and 150 μl of collagenase (from clostridium, 0.2 mg/ml), and reacted at room temperature for 20 minutes. After stopping the reaction by adding 500 μl of 6% citric acid, 1.5 ml of ethyl acetate was added and the supernatant was measured for absorbance at 320 nm using a UV/Vis spectrophotometer (Optizen Pop, Mecasys, Korea). measured. Collagenase inhibitory effect was expressed as IC ₅₀ (inhibitory concentration of 50%), and EGCG (epigallocatechin gallate) was used as a positive control.

7. Tyrosinase inhibitory activity

In order to measure the tyrosinase inhibitory effect of the tyrosinase extract, 21 μl of the sample and 49 μl of 10 mM sodium phosphate buffer (pH 6.8) were added to a 96-well plate, followed by L-tyrosine (0.1 μl). 70 μl of tyrosinase (200 unit/ml) diluted with 70 μl of (mg/ml) and 70 μl of diluted tyrosinase (200 unit/ml) were added and reacted for 20 minutes at 37° C. Absorbance was measured. The tyrosinase inhibitory effect was expressed as IC ₅₀ , and arbutin was used as a positive control.

8. Statistical processing

Two-way ANOVA analysis was performed to confirm the antioxidant effect, total phenolic and total flavonoid contents, and collagenase and tyrosinase inhibitory effects of the leaves and rhizomes of schismatics according to the steaming conditions. The data of all experiments were performed at least 3 times and expressed as means±standard deviation (SD). For statistical processing, significance was verified by Duncan's Multiple Range Test (DMRT) using comprehensive software IBM SPSS Statistics v24 (SPSS, USA), and statistical significance was analyzed at 5% ( p < 0.05).

Example 1. Antioxidant activity assay

1-1. DPPH radical scavenging activity

As a result of measuring the DPPH radical scavenging activity of the extract of the leaf or rhizome steamed under various temperature and time conditions and the extract of the leaf or rhizome that was not steamed (control), the steamed The RC ₅₀ value of the extract of Dungula leaf was reduced by about 1.7 to 3 times compared to the control group, showing high antioxidant activity.

In addition, the RC ₅₀ value increased compared to the control when steaming the rhizomes of 60 ° C, 80 ° C or 100 ° C for 1 hour or 3 hours, but steaming at 60 ° C, 80 ° C or 100 ° C for more than 6 hours When compared to the control group, the RC ₅₀ value decreased, especially when steamed at 120 ° C for 12 hours, the RC ₅₀ value was 0.33 mg / ml, and when steamed for 24 hours, the RC ₅₀ value was 0.29 mg / ml, showing high antioxidant activity. were seen (Table 1).

1-2. ABTS radical scavenging activity

As a result of measuring the ABTS radical scavenging activity of the extract of the leaf or rhizome steamed under various temperature and time conditions and the extract of the leaf or rhizome that was not steamed (control), the steamed The RC ₅₀ value of the extract of Dungula leaf was significantly decreased compared to the control group, especially when steamed at 120 ° C for 12 hours, the RC ₅₀ value was 0.15 mg / ml, and when steamed for 24 hours, the RC ₅₀ value was 0.14 mg / ml. showed high antioxidant activity.

In addition, when steaming the rhizomes of sagebrush at 60 ° C, 80 ° C, or 100 ° C for 1 hour or 3 hours, the RC ₅₀ value slightly increased compared to the control group, but when steamed for 6 hours or more, the RC ₅₀ value increased compared to the control group. In particular, when steamed at 120 ° C. for 12 or 24 hours, the RC ₅₀ value was 0.42 mg / ml, showing high antioxidant activity (Table 1).

Example 2. Analysis of total phenol and flavonoid content

2-1. total phenol content

The total phenolic content of the extracts of the leaves or rhizomes of P. japonica that were steamed under various temperature and time conditions and the extracts of the leaves or rhizomes that were not steamed (control group) were measured.

First, there was no significant difference in the flavonoid content of the leaf extract of the sagebrush chinensis in the control group and the group steamed at 60 ° C or 80 ° C, but the phenol content increased from the steaming condition at 100 ° C for 12 hours, especially at 120 ° C for 12 hours. During the steaming treatment, the total phenol content was 33.23 ± 0.97 mg·GAE/g, which was 2.2 times higher than that of the control group.

In addition, the phenolic content of the rhizome extract of the sagebrush was not measured in the control group and the group steamed at 60 ° C, 80 ° C or 100 ° C for 1 hour, 3 hours or 6 hours, but the phenol content from the steaming condition of 12 hours at 100 ° C It was possible to measure, and in particular, when steamed at 120 ° C. for 12 hours, it was confirmed that the phenol content was the highest at 15.78 ± 0.79 mg GAE / g (Table 2).

2-2. Total flavonoid content

The flavonoid contents of the extracts of the leaves or rhizomes of the steamed rhizomes and those of the leaves or rhizomes (control group) that were not steamed were measured under various temperature and time conditions.

First, there was no significant difference in the flavonoid content of the leaf extract of the leaf extract from the control group and the group steamed at 60 ° C or 80 ° C, but the flavonoid content increased when steamed at 100 ° C or 120 ° C, especially at 120 ° C. When steamed for 12 hours, it was confirmed that the flavonoid content was 18.76 ± 0.38 mg·QE/g, about 1.7 times higher than that of the control group.

In addition, the flavonoid content of the extract of the rhizome of the rhizome was not measured in the control group and the group steamed at 60 ° C, 80 ° C or 100 ° C, but it was possible to measure the flavonoid content from the condition steamed at 120 ° C for 6 hours. , In particular, when steamed at 120 ° C. for 12 hours, the phenol content was 7.20 ± 0.04 mg·QE / g, confirming that phenol was included the most (Table 2).

Through the above results, when the extract is prepared by steaming the leaves or rhizomes at 120 ° C. for 12 hours and then ultrasonicating them, DPPH and ABTS radical scavenging activity and total phenolic and flavonoid content enhancement effects are confirmed. did

Then, the possibility of application as a cosmetic material was evaluated by confirming the wrinkle and whitening effect of the extract of the leaf or rhizome steamed at 120 ° C for 12 hours.

Example 3. Collagenase inhibitory activity

Collagen accounts for 70-80% of skin epithelial cells, and is a component closely related to skin aging, such as maintaining the structure and elasticity of the extracellular matrix. Collagen is known to be degraded by collagenase, and collagenase decomposition directly affects the decrease in elasticity of connective tissue and the formation of wrinkles, so collagenase inhibitory activity is an important part of skin elasticity.

As a result of measuring the collagenase inhibitory activity using the extract of the leaf or rhizome steamed at 120 ° C. for 12 hours, the group treated with the extract of the leaf or rhizome that was not steamed (control group ), it was confirmed that the IC ₅₀ value decreased by about 2 times in the group treated with the extract of the steamed leaf or rhizome, compared to the steamed leaf or rhizome. It was expected to be effective in improving skin wrinkles due to increased activity (Table 3).

Example 4. Tyrosinase inhibitory activity

Tyrosinase is an enzyme that oxidizes and decomposes tyrosine in melanocytes present in the basal layer of the skin to promote melanine production. Inhibition of tyrosinase activity plays an important role in skin whitening. do

As a result of measuring the tyrosinase inhibitory activity using the extract of the leaf or rhizome steamed at 120 ° C. for 12 hours, the group treated with the extract of the leaf or rhizome that was not steamed (control group ), the IC ₅₀ value decreased in the group treated with the extract of the steamed rhizome or leaf or rhizome treated with the steaming treatment, and in particular, the IC ₅₀ value in the group treated with the extract of the steamed It was confirmed that about 6 times decreased (Table 4). Through this, it was thought that when steaming the leaves or rhizomes of the leaf or rhizome, the tyrosinase inhibitory activity would increase and be effective in skin whitening.

Claims (6)

A cosmetic composition for skin whitening and wrinkle improvement comprising, as an active ingredient, an extract of a leaf of a parsnip parsnips prepared by steaming and ultrasonically extracting the leaves of cicadas. delete The cosmetic composition according to claim 1, wherein the cosmetic composition is cream, softening lotion, nutrient lotion, pack, essence, hair tonic, shampoo, conditioner, hair conditioner, hair treatment, gel, skin lotion, skin softener, skin toner, astringent, lotion From the group consisting of milk lotion, moisture lotion, nutrient lotion, massage cream, nutrient cream, moisture cream, hand cream, foundation, nutrient essence, sunscreen, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser A cosmetic composition for skin whitening and wrinkle improvement, characterized in that it has a selected formulation. A health functional food composition for skin whitening and wrinkle improvement comprising, as an active ingredient, an extract of a leaf of a sagebrush prepared by ultrasonic extraction after steaming the leaves of the sagebrush. A pharmaceutical composition for the prevention or treatment of melanin hyperpigmentation diseases comprising, as an active ingredient, an extract of the leaves of St. The method of claim 5, wherein the melanin hyperpigmentation disease is freckles, senile spots, liver spots, melasma, brown or dark spots, solar pigment spots, cyanic melasma, hyperpigmentation after drug use, pregnancy brown spots (gravidic spots) chloasma), or a pharmaceutical composition for the prevention or treatment of melanin hyperpigmentation disease characterized by hyperpigmentation after inflammation due to wounds or dermatitis.