KR102549076B1 - Composition for skin whitening comprising mixture of fermented ginseng leaf extract and Miscanthus sinensis extract as effective component - Google Patents

Abstract

The present invention relates to a composition for skin whitening containing a mixture of a fermented product of a ginseng leaf extract and a silver grass extract as an active ingredient, wherein the mixture of the fermented product of a ginseng leaf extract and a silver grass extract, which are active ingredients, has a tyrosinase inhibitory effect Since it has a remarkable effect compared to fermented ginseng leaf extract or silver grass extract, the composition of the present invention containing the active ingredient is a cosmetic for skin whitening, a health functional food for improving melanin hyperpigmentation disease, or a melanin hyperpigmentation disease. It can be useful as a therapeutic agent.

Description

Composition for skin whitening comprising mixture of fermented ginseng leaf extract and Miscanthus sinensis extract as effective component}

The present invention relates to a composition for skin whitening containing a mixture of a fermented product of a ginseng leaf extract and a silver grass extract as an active ingredient.

In modern society, there is an increasing recognition that appearance is one of social competitiveness, and the desire for a healthy life and beauty is getting stronger as the average life expectancy of humans increases as medical technology and income levels improve. Based on these changes of the times and the increase in interest, the market size of the cosmetics field is rapidly expanding, and related research is being conducted from various angles.

In particular, interest in skin whitening, such as pigmentation-type melasma or freckles, is increasing. Various factors are involved in determining the skin color of a person. Among them, the activity of melanocytes that produce melanin pigment, the distribution of blood vessels, the thickness of skin, and pigment-related factors inside and outside the human body, such as carotenoids and bilirubin, are important.

Among them, the most important factor is a pigment called melanin, which is produced by the action of various enzymes such as tyrosinase in melanocytes in the human body. It is known that pigmentation of the skin is caused by stimuli such as ultraviolet rays or inflammation, and also by factors such as genetic factors, hormones, foods, and chemicals. In particular, inflammation-related factors such as prostaglandins, histamine, alpha-MSH, and NO (Nitric oxide) are involved in abnormal skin pigmentation symptoms such as melasma, freckles, and pigmentation, resulting in melanocyte formation. ) is well known to activate melanin production in In addition, there is also a report that hyperpigmentation is accompanied by immunological mediators even in the process of tissue damage and regeneration.

Looking at melanin pigment synthesis in detail, when melanocytes are stimulated by various factors, tyrosinase produces melanin in melanosomes. That is, tyrosine in melanocytes is converted into dopa by the action of tyrosinase, then converted into dopaquinone by the action of dopaoxidase, and then tyrosinase-related protein-1 (tyrosinase-related protein-1). Melanin is produced by the action of protein-1 (hereinafter referred to as TRP-1) and tyrosinase-related protein-2 (hereinafter referred to as TRP-2). In this series of reactions, since tyrosinase activates the rate-limiting reaction of the melanin pigment formation process, melanin formation is primarily controlled by the activity and expression of tyrosinase. In addition, TRP-2, known as DOPA chromium transmutase, activates the reaction of DOPA chromium to 5,6-dihydroxyindole-2-carboxylic acid (DHICA), and TRP-1 converts DHICA to carboxyl indole-quinone. oxidize to As a result, TRP-1 and TRP-2 are involved in the polymerization reaction, which is the final stage of formation of melanin, an insoluble pigment. It has been reported that these tyrosinase, TRP-1 (tyrosinase-related protein-1), and TRP-2 (tyrosinase-related protein-2) increase their activity by inflammatory mediators and increase the synthesis of melanin. .

Currently developed whitening agents include kojic acid, arbutin, glutathione, vitamin C, etc., but they do not have a whitening effect satisfactory to consumers, and their usage is limited due to skin safety. Therefore, there is an increasing demand for the development of cosmetic products effective for skin whitening using natural materials whose stability has been verified.

On the other hand, Korean Patent No. 2295904 discloses a cosmetic composition for skin whitening containing an extract or a fraction thereof as an active ingredient, and Korean Patent No. 1936294 discloses an extract of A. However, a composition for skin whitening containing a mixture of a fermented product of the ginseng leaf extract and a silver grass extract of the present invention as an active ingredient has not been disclosed.

The present invention has been derived from the above needs, and provides a composition for skin whitening containing a mixture of a fermented ginseng leaf extract and a silver grass extract as an active ingredient, and a mixture of the fermented ginseng leaf extract and a silver grass extract. By confirming the skin whitening effect of, the present invention was completed.

In order to solve the above problems, the present invention provides a cosmetic composition for skin whitening containing a mixture of fermented ginseng leaf extract and silver grass ( Miscanthus sinensis ) extract as an active ingredient.

In addition, the present invention provides a health functional food composition for preventing or improving melanin hyperpigmentation disease containing a mixture of fermented ginseng leaf extract and silver grass extract ( Miscanthus sinensis ) as an active ingredient.

In addition, the present invention provides a pharmaceutical composition for the prevention or treatment of melanin hyperpigmentation disease containing a mixture of fermented ginseng leaf extract and silver grass extract ( Miscanthus sinensis ) as an active ingredient.

The present invention relates to a composition for skin whitening containing a mixture of a fermented product of a ginseng leaf extract and a silver grass extract as an active ingredient, wherein the mixture of the fermented product of a ginseng leaf extract and a silver grass extract, which are active ingredients, has a tyrosinase inhibitory effect Since it has a remarkable effect compared to fermented ginseng leaf extract or silver grass extract, the composition of the present invention containing the active ingredient is a cosmetic composition for skin whitening, a health functional food composition for preventing or improving melanin hyperpigmentation disease, or melanin It can be usefully used as a pharmaceutical composition for preventing or treating hyperpigmentation diseases.

1 is a result confirming the tyrosinase inhibitory effect of the mixture of the fermented product of the ginseng leaf extract and the silver grass extract of the present invention. @ means that the tyrosinase inhibitory activity of the mixture of the fermented product of the ginseng leaf extract and the silver grass extract was statistically significantly increased compared to the fermented product of the ginseng extract, p <0.05. ** means that the tyrosinase inhibitory activity of the mixture of the fermented product of the ginseng leaf extract and the silver grass extract was statistically significantly increased compared to the silver grass extract, p <0.01.

In order to provide the object of the present invention, the present invention provides a cosmetic composition for skin whitening containing a mixture of fermented ginseng leaf extract and silver grass ( Miscanthus sinensis ) extract as an active ingredient.

In the present invention, the skin whitening effect is an effect of improving or preventing dark skin, melasma, freckles, and dark circles caused by various factors such as exposure to ultraviolet rays, changes in hormonal balance, and genetic programs, and making the skin transparent and beautiful. It refers to the effect of maintaining beautiful skin with a sense of clearness or transparency, the effect of reducing the dullness of the skin and increasing the luster and tightness of the skin.

The solvent of the extract may be water, C ₁ ~ C ₄ lower alcohol, or a mixture thereof, preferably, the solvent of the ginseng leaf extract is water, and the solvent of the silver grass extract is ethanol, but is not limited thereto.

The mixture may be a mixture of fermented ginseng leaf extract: silver grass extract in a weight ratio of 6:3 to 25, preferably a fermented product of ginseng leaf extract: silver grass extract in a weight ratio of 6:3 to 10 More preferably, fermented product of ginseng leaf extract: silver grass extract may be mixed in a weight ratio of 6: 3 to 5, and most preferably fermented product of ginseng leaf extract: silver grass extract mixed in a weight ratio of 6: 4 but is not limited thereto.

The fermented product of the ginseng leaf extract may be fermented by inoculating the ginseng leaf extract with Leuconostoc citreum, and preferably fermented by inoculating the ginseng leaf extract with Leuconostoc citreum KCTC3526 More preferably, after inoculating ginseng leaf extract with Leuconostoc citreum KCTC3526, it may be fermented for 48 to 96 hours at 35 ~ 40 ℃, most preferably, Leuconostoc citreum KCTC3526 in ginseng leaf extract After inoculation, it was fermented at 37 ° C. for 72 hours, but is not limited thereto.

The cosmetic composition of the present invention can be prepared in any formulation commonly prepared in the art, for example, solution, suspension, emulsion, paste, gel, cream, lotion, powder, oil, powder foundation, emulsion It can be formulated into foundation, wax foundation and spray, etc., and more specifically, skin, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrient lotion, massage cream, nutrient cream, eye cream, Moisture cream, hand cream, essence, nutrient essence, pack, cleansing foam, cleansing water, cleansing lotion, cleansing cream, body lotion, body cleanser, soap or powder may be manufactured in cosmetic formulations, but is not limited thereto.

When the formulation of the cosmetic composition of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide as a carrier component this can be used

When the formulation of the cosmetic composition of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additionally chlorofluorohydro propellants such as carbon, propane/butane or dimethyl ether.

When the formulation of the cosmetic composition of the present invention is a solution or emulsion, a solvent, solubilizing agent or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene fatty acid esters of glycol, 1,3-butylglycol oil, glycerol aliphatic esters, polyethylene glycol or sorbitan.

When the formulation of the cosmetic composition of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester , microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracanth, and the like can be used.

The cosmetic composition of the present invention includes ingredients commonly used in cosmetic compositions in addition to the above active ingredients, such as fatty substances, organic solvents, solubilizers, thickeners and gelling agents, softeners, antioxidants, suspending agents, stabilizers, foaming agents ( foaming agents), fragrances, surfactants, water, ionic or nonionic emulsifiers, fillers, sequestering and chelating agents, preservatives, vitamins, blocking agents, humectants, essential oils, dyes, pigments, hydrophilic or lipophilic actives , conventional adjuvants such as lipid vesicles, and carriers.

In addition, the present invention provides a health functional food composition for preventing or improving melanin hyperpigmentation disease containing a mixture of fermented ginseng leaf extract and silver grass extract ( Miscanthus sinensis ) as an active ingredient.

As used herein, the term “melanin hyperpigmentation” means that certain parts of the skin or nails become black or darker than other parts due to an excessive increase in melanin. The melanin hyperpigmentation diseases include freckles, senile spots, liver spots, melasma, brown or dark spots, solar pigment spots, cyanic melasma, hyperpigmentation after drug use; post-inflammatory hyperpigmentation due to scratches, burns or dermatitis; or gestational brown spots (gravidic chloasma), etc., but are not limited thereto.

When using the health functional food composition of the present invention as a food additive, the health functional food composition may be added as it is or used together with other foods or food ingredients, and may be appropriately used according to conventional methods. The amount of the active ingredient can be appropriately used depending on the purpose of its use (prevention or improvement).

There is no particular limitation on the type of health functional food. Examples of foods to which the health functional food composition can be added include meat, sausages, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum, dairy products including ice cream, various soups, beverages, tea There are drinks, alcoholic beverages, and vitamin complexes, and includes all health foods in a conventional sense.

In addition, the health functional food composition of the present invention can be made into food, particularly functional food. The functional food of the present invention includes components commonly added during food preparation, and includes, for example, proteins, carbohydrates, fats, nutrients, and seasonings. For example, when prepared as a drink, natural carbohydrates or flavoring agents may be included as additional ingredients in addition to active ingredients. The natural carbohydrates are monosaccharides (eg, glucose, fructose, etc.), disaccharides (eg, maltose, sucrose, etc.), oligosaccharides, polysaccharides (eg, dextrins, cyclodextrins, etc.) or sugar alcohols (eg, maltose, sucrose, etc.) , xylitol, sorbitol, erythritol, etc.) are preferred. As the flavoring agent, natural flavoring agents (eg, thaumatin, stevia extract, etc.) and synthetic flavoring agents (eg, saccharin, aspartame, etc.) may be used.

In addition to the health functional food composition, various nutrients, vitamins, electrolytes, flavors, colorants, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonic acid It may further contain a carbonation agent used in beverages and the like. The ratio of the components to be added is not very important, but is generally selected in the range of 0.01 to 0.1 parts by weight based on 100 parts by weight of the health functional food composition of the present invention.

In addition, the present invention provides a pharmaceutical composition for the prevention or treatment of melanin hyperpigmentation disease containing a mixture of fermented ginseng leaf extract and silver grass extract ( Miscanthus sinensis ) as an active ingredient.

The pharmaceutical composition of the present invention may include pharmaceutically acceptable carriers in addition to active ingredients, and such carriers are commonly used in preparation, and include lactose, dextrose, sucrose, sorbitol, mannitol, starch, gum acacia, Calcium phosphate, alginate, gelatin, calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, water, syrup, methyl cellulose, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and minerals. oils and the like, but are not limited thereto. The pharmaceutical composition of the present invention may further include a lubricant, a wetting agent, a sweetening agent, a flavoring agent, an emulsifying agent, a suspending agent, a preservative, and the like in addition to the above components.

A suitable dosage of the pharmaceutical composition according to the present invention is variously prescribed depending on factors such as formulation method, administration method, patient's age, weight, sex, pathological condition, food, administration time, administration route, excretion rate and response sensitivity. It can be.

The pharmaceutical composition of the present invention can be administered orally or parenterally, and in the case of parenteral administration, it can be administered by topical application to the skin, intravenous injection, subcutaneous injection, intramuscular injection, intraperitoneal injection, transdermal administration, etc. Considering that the pharmaceutical composition of the present invention is applied for the treatment or prevention of melanin hyperpigmentation disease, it is preferably applied topically to the skin.

The concentration of the active ingredient included in the composition of the present invention can be determined in consideration of the purpose of treatment, the condition of the patient, the required period, etc., and is not limited to a specific range of concentration.

The pharmaceutical composition of the present invention is prepared in unit dosage form by formulating it using a pharmaceutically acceptable carrier or excipient according to a method that can be easily performed by those skilled in the art, or It can be prepared by incorporating into a dose container. At this time, the formulation may be prepared as any one formulation selected from injections, creams, patches, sprays, ointments, warnings, lotions, liniments, pastas, and cataplasmas, and may additionally include a dispersing agent or a stabilizer. there is.

Hereinafter, the present invention will be described in more detail using preparation examples and examples. These Preparation Examples and Examples are only for explaining the present invention in more detail, and it is obvious to those skilled in the art that the scope of the present invention is not limited thereto.

Preparation Example 1. Fermented product of ginseng leaf extract

The ginseng leaves of the present invention were washed, completely dried naturally, and then pulverized and used. After adding 1 L of water to 20 g of crushed ginseng leaves, hot water extraction was performed at 100 ° C. for 2 hours. After that, it was sterilized using an autoclave. Then, 500mL of Leuconostoc citreum (KCTC3526) culture medium cultured to an OD ₆₀₀ value of 0.6 to 0.8 was inoculated into the sterilized ginseng leaf hot water extract, fermented at 37°C for 72 hours, and then the fermentation process was terminated After boiling for 5 minutes, additional fermentation was prevented from occurring. The fermented product was filtered using a filter paper, the solvent was removed using an EYELA evaporator, and then lyophilized and stored at minus 4° C., and used as a sample for the experiment.

Preparation Example 2. Silver grass extract

The grass grass of the present invention ( Miscanthus sinensis ) used an outpost, was washed, completely dried naturally, and then pulverized and used. After adding 2L of 80% (v/v) ethanol to 20g of pulverized silver grass powder, soaking for 72 hours for extraction, filtering with paper filter paper, completely removing the solvent from the filtrate using a vacuum concentrator, and then storing it in a refrigerator for the experiment. was used as a sample.

Preparation Example 3. A mixture of fermented product of ginseng leaf extract and silver grass extract

After diluting the fermented product of the ginseng leaf extract of Preparation Example 1 and the silver grass extract of Preparation Example 2 with water to 5000 μg/mL, respectively, the fermented product of the ginseng leaf extract: silver grass extract 6:4, 4:6 or 2: 8 by weight.

Example 1. Tyrosinase inhibitory activity

To evaluate the whitening efficacy, DOPA oxidation inhibition assay was performed.

Specifically, after mixing 80 μl of the sample solution diluted to 5000 μg/mL, 40 μl of 10 mM L-DOPA (3,4-Dihydroxy-L-phenylalanine) and 40 μl of mushroom tyrosinase (125 U/mL), After reacting at 25° C. for 30 minutes, the absorbance of the DOPA chrome produced was measured at 490 nm using a microplate reader. The group to which tyrosinase was not added was used as a blank test group, and the group to which no sample solution was added was used as a control group, and the enzyme activity inhibition rate was calculated according to the following formula 1.

[Equation 1]

Tyrosinase activity inhibition rate (%) = {100-(b-b')/(a-a')}X100

a: Absorbance after reaction of blank sample solution

b: Absorbance after reaction of sample solution

a', b': absorbance measured by replacing tyrosinase with buffer

As a result, as shown in FIG. 1, it was confirmed that the tyrosinase inhibitory activity of these mixtures was significantly increased compared to the fermented product of ginseng extract or the silver grass extract. In particular, the tyrosinase inhibitory activity of the mixture in which the fermented product of ginseng extract and the extract of Pampas grass was mixed at a weight ratio of 6:4 was more excellent than that of the mixture mixed at a weight ratio of 4:6 and 2:8.

Claims (8)

A cosmetic composition for skin whitening containing a mixture of fermented ginseng leaf extract and silver grass ( Miscanthus sinensis ) extract as an active ingredient. The cosmetic composition for skin whitening according to claim 1, wherein the solvent of the extract is water, C ₁ ~ C ₄ lower alcohol or a mixture thereof. The cosmetic composition for skin whitening according to claim 1, wherein the mixture is a mixture of fermented ginseng leaf extract and silver grass extract in a weight ratio of 6:3 to 25. The cosmetic composition for skin whitening according to claim 3, wherein the mixture is a mixture of fermented ginseng leaf extract and silver grass extract in a weight ratio of 6:3 to 5. The cosmetic composition for skin whitening according to claim 4, wherein the fermented product of the ginseng leaf extract is fermented by inoculating the ginseng leaf extract with Leuconostoc citreum . A health functional food composition for preventing or improving melanin hyperpigmentation disease, containing a mixture of fermented ginseng leaf extract and silver grass ( Miscanthus sinensis ) extract as an active ingredient. The method of claim 6, wherein the melanin hyperpigmentation disease is freckles, senile spots, liver spots, melasma, brown or dark spots, solar pigment spots, cyanic melasma, hyperpigmentation after drug use, scratches, burns or dermatitis A health functional food composition for preventing or improving melanin hyperpigmentation disease, which is characterized by hyperpigmentation or gestational brown spots (gravidic chloasma) due to inflammation. A pharmaceutical composition for the prevention or treatment of melanin hyperpigmentation diseases containing a mixture of fermented ginseng leaf extract and silver grass extract ( Miscanthus sinensis ) as an active ingredient.

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