KR20200139893A - Antioxidnat and antibacterial composition comprising Quercus acuta Thunb acorn extract - Google Patents

Abstract

The present invention relates to an antioxidant and antibacterial composition in which a concentration of a Quercus acuta acron extract is 0.312 to 0.019 mg/mL, and having antibacterial activity on bacteria selected from a group consisting of Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus.

Description

Antioxidant and antibacterial composition comprising Quercus acuta Thunb acorn extract as an active ingredient

The present invention relates to a composition for antioxidant and antibacterial comprising a red thorn acorn extract as an active ingredient, and more specifically, the concentration of red thorn acorn extract is 0.312 mg/ml to 0.019 mg/ml, Escherichia coli , Pseudomonas aeruginosa ) and It relates to an antioxidant and antibacterial composition having antibacterial activity against bacteria selected from the group consisting of Staphylococcus aureus .

Thorn trees are evergreen broad-leaved trees belonging to the Oak family, and in Korea, they are known to grow natively in the southern regions, and the Western Regional Forest Service and local governments actively carried out nursery and afforestation projects for Nansang green broad-leaved trees from 1997 to the present. This has already exceeded about 200 ha and continues to increase.

The total distribution area of red thorns existing in Korea is estimated to be about 1,832 ha, and in the southern regions, acorns of thorns are made and consumed with mud (Patent Document 1), and the chemical composition of acorns is generally about 70 It has been reported that% is composed of starch and about 6 to 9% is composed of tannin (Non-Patent Document 1).

Tannins are naturally astringent and bitter, but these tannins are a kind of polyphenol and are known to be a major ingredient in antioxidant effects, so they are considered to be valuable as functional resources other than edibles.

Until now, the results of the phenol content present in oak trees including thorns have been disclosed (Non-Patent Literature 1 to Non-Patent Literature 3), but most of the studies have been conducted on leaves, and even this is limited to some species, and has antioxidant functionality. Substance-related research results are rare.

On the other hand, patent documents include atopic dermatitis improving agent using red thorn leaf extract (Patent Document 2), a composition for preventing and treating metabolic disorders related to hyperuricemia and hyperuricemia containing red thorn extract as an active ingredient (Patent Document 3), red thorn Antioxidant and anticancer compositions containing tree extract as an active ingredient (Patent Document 4), etc., are disclosed, but the patent documents also only use extracts such as red thorn leaves as an active ingredient. That is, it is not disclosed about using the extract of acorn as an active ingredient.

Korean Patent Publication No. 10-0917946 Korean Patent Application Publication No. 10-2011-0055940 Republic of Korea Patent Publication No. 10-1717698 Republic of Korea Patent Publication No. 10-2009-0118154

The Korean Journal of Food And Nutrition (2016) Vol.29 No.1 pp.58-64 Journal of Medicinal Plants Research (2018) Vol. 6(39), pp. 5228-5239. Comparison of phenolic compounds content in indeciduous Quercus species Food Research International (2018). Vol 114, pp. 208-213

The present inventors conducted a study to solve the problems of the prior art as described above, and six kinds of thorns that grow in Korea (thorn, jong thorn, thorn thorn, thorn thorn, red thorn, red thorn ) Of the seeds, that is, by evaluating the antioxidant and antibacterial activity of the acorn, a comparative analysis between several species was performed, and the present invention was completed.

Accordingly, it is an object of the present invention to provide a composition for antioxidant and antibacterial comprising acorn extract derived from specific thorns having excellent antioxidant activity and antibacterial activity.

The present invention provides a composition for antioxidant and antibacterial comprising a red thorn acorn extract as an active ingredient in order to achieve the above object.

The present invention also provides a composition for antioxidant and antibacterial, wherein the red thorn acorn extract is an ethanol extract of red thorn acorn.

The present invention also provides a composition for antioxidant and antibacterial in which the concentration of the red thorn acorn extract is 0.312 mg/ml to 0.019 mg/ml.

In the present invention, the red thorn acorn extract is Escherichia coli ), Pseudomonas aeruginosa and Staphylococcus ( Staphylococcus. aureus ) provides an antioxidant and antibacterial composition having an antibacterial activity against one or more bacteria selected from the group consisting of.

The present invention also provides a composition for antioxidant and antibacterial, wherein the composition is a health functional food composition.

The present invention also provides a composition for antioxidant and antibacterial, wherein the composition is a pharmaceutical composition.

The present invention also provides a composition for antioxidant and antibacterial, wherein the composition is a cosmetic composition.

The composition for antioxidant and antibacterial comprising the red thorn acorn extract of the present invention as an active ingredient has the effect of showing remarkably high antioxidant activity and antibacterial activity compared to the acorn extract derived from other types of thorn trees.

Figure 1 shows the DPPH antioxidant activity (%) of the ethanol extract of acorns of six kinds of thorns.
Figure 2 shows the ABTS antioxidant activity (absorbance, 450nm) of the ethanol extract of acorns of six kinds of thorns.
Figure 3 shows the antibacterial activity against S. aureus of the ethanol extract of acorns of six kinds of thorns, and in Figure 3, "S. lococus” means Staphylococcus aureus.
Figure 4 shows the antibacterial activity against P. aeruginosa of the ethanol extract of acorns of six kinds of thorns.
Figure 5 shows the antibacterial activity against E. coli of the ethanol extract of acorns of six kinds of thorns.

The present invention relates to a composition for antioxidant and antibacterial comprising a red thorn acorn extract as an active ingredient.

Quercus acuta THUNB.) is called a red thorn tree because of its red color. It is distributed in Jeollanam-do and Jeju-do because of its poor cold resistance, and grows vertically at 170-500m in height. It grows mainly in the sunny areas of mountains and valleys, but has some strength to withstand in the shade, has excellent composition and pollution resistance, and the wood is heavy and does not split well, and has good preservation properties, so it is used as equipment, machinery, vehicle, and ship materials. Has become.

The red thorn acorn extract contained in the antioxidant and antibacterial composition of the present invention is one or more solvent extracts selected from the group consisting of water, C1-C4 alcohol, acetone, ethyl acetate, n-butanol, chloroform and methylene chloride. I can.

The red thorn acorn extract contained in the antioxidant and antibacterial composition of the present invention may preferably be an ethanol extract, the concentration of the ethanol is 70 to 99% (v / v), preferably 90 to 99% ( v/v), more preferably 95% (v/v).

The method of extracting the red thorn acorn extract may include both a method of immersing red thorn and a method of distilling the tree when participating in an extraction solvent, and the extraction method by immersion includes cold sedimentation, warming, ultrasound, reflux, etc. The method of can be applied. The extraction time may be performed for 1 day or more, preferably 3 days or more, preferably 3 days, and may vary depending on conditions such as an extraction solvent and extraction temperature.

The extraction may be performed once or more times to obtain a larger amount of the active ingredient, and an extract obtained by combining the respective extracts obtained by extracting several times may be used.

The extract extracted by the above method may be used as it is, but may be used in the form of concentrated X. Preferably, it is used in a concentrated form so that it can be used in a sufficient concentration. The concentration may be performed by concentrating the extract under reduced pressure at, for example, 30°C. It may be concentrated and then freeze-dried to be used as a freeze-dried product.

The red thorn acorn extract contained in the antioxidant and antibacterial composition of the present invention is at a concentration of 0.312 mg/ml to 0.019 mg/ml, preferably 0.078 mg/ml to 0.019 mg/ml, thorns, closing price Compared to other thorns such as birch, birch, prickly pear, red thorn, etc., it has significantly higher DPPH activity.

The red thorn acorn extract contained in the antioxidant and antibacterial composition of the present invention is Escherichia coli , Pseudomonas aeruginosa ) and At least one selected from the group consisting of Staphylococcus aureus , preferably can exhibit antibacterial activity against the three kinds of bacteria.

The antioxidant and antibacterial composition of the present invention may be a health functional food composition. In the present invention, the term "health functional food" is used as a raw material or ingredient having useful functions for the human body according to the Health Functional Food Act No. It means a manufactured and processed food, and "functional" means ingesting for the purpose of obtaining useful effects for health purposes such as physiological action or controlling nutrients on the structure and function of the human body.

The health functional food composition may be formulated into a conventional health functional food formulation known in the art. The health functional food may be prepared, for example, as a powder, granule, tablet, capsule, suspension, emulsion, syrup, liquid, extract, tea, jelly, extract, or beverage.

Any carrier or additive known to be usable in the art for preparing a formulation to be prepared may be used as the food pharmaceutically acceptable carrier or additive.

When the health functional food is a beverage, it may additionally contain various flavoring agents or natural carbohydrates, as in ordinary beverages. The natural carbohydrates include monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, and polysaccharides such as dextrin and cyclodextrin, sugar alcohols such as xylitol, sorbitol, and erythritol, but are not limited thereto.

The present invention may also be a composition for antioxidant and antibacterial, wherein the composition is a pharmaceutical composition, and may be formulated into a conventional pharmaceutical formulation known in the art.

The pharmaceutical composition may be formulated and administered in any formulation including, but not limited to, oral formulations, injections, suppositories, transdermal formulations, external formulations, and nasal formulations, but is preferably a solution, suspension, or powder. , Granules, tablets, capsules, pills, or may be formulated into a dosage form for oral administration such as an extract.

When formulated into each of the above formulations, it can be prepared by adding a pharmaceutically acceptable carrier or additive necessary for the manufacture of each formulation. Typically, when formulated into a dosage form for oral administration, one or more of a diluent, a lubricant, a binder, a disintegrant, a sweetener, a stabilizer, and a preservative can be selected and used as the carrier. As an additive, flavoring, vitamins, and antioxidants It can be used by selecting one or more of the agents.

The carriers and additives may be pharmaceutically acceptable, and specifically, lactose, corn starch, soybean oil, microcrystalline cellulose, or mannitol as a diluent, magnesium stearate or talc as a lubricant, and polyvinylpyrrolidone as a binder. Or hydroxypropyl cellulose is preferable.

In addition, as the disintegrant, calcium carboxymethylcellulose, sodium starch glycolate, potassium polyacrylate, or crospovidone, as a sweetener, sucrose, fructose, sorbitol, or aspartame, and as the stabilizer, sodium carboxymethylcellulose, beta-cyclo Dextrin, white lead, or xanthan gum, and the preservative is preferably methyl paraoxybenzoate, propyl paraoxybenzoate, or potassium sorbate.

In addition, in addition to the above ingredients, in order to enhance the taste as well-known additives, natural flavors such as plum flavor, lemon flavor, pineapple flavor, herbal flavor, natural fruit juice, chlorophyllin, flavonoids, etc., fructose, honey, sugar alcohol, sugar A sweetening component such as, or an acidifying agent such as citric acid or sodium citrate, may be mixed and used.

In the present invention, the composition may also be a cosmetic composition, which is an antioxidant and antibacterial composition, and may be prepared in a specific formulation by a method well known in the cosmetic field, and may further include cosmetically acceptable additives. .

The additives include stabilizers, solubilizers, emulsifiers, vitamins, pigments, flavors, or combinations thereof, but are not limited thereto, and may be modified and used according to the type of formulation to be prepared.

The cosmetic composition may be prepared in any formulation conventionally prepared in the art, for example, solution, suspension, emulsion, paste, gel, cream, lotion, surfactant-containing cleansing, liquid foundation, and cream foundation. It may be formulated as, but is not limited thereto.

When the formulation of the cosmetic composition is a solution or emulsion, a solvent, a solubilizing agent, or an emulsifying agent is used as a carrier component, such as water, ethanol, carboxylvinyl polymer, propylene glycol, 1,3-butylene glycol, and glycerin. Aliphatic esters, polyethylene hardened castor oil, preservatives, or sorbitan fatty acid esters.

When the formulation of the cosmetic composition is a suspension, a liquid diluent such as water, fragrance, concentrated glycerin, butylene glycol or propylene glycol as a carrier component, ethoxylation isosterayl alcohol, polyoxyethylene sorbitol ester, or polyoxyethylene sorbate Emulsifying suspending agents such as non-aqueous esters, microcrystalline cellulose, carboxyvinyl polymers, thickeners or preservatives may be used.

When the formulation of the cosmetic composition is cleansing containing a surfactant, as a carrier component, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, fatty alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives, or ethoxylated glycerol fatty acid esters may be used.

Hereinafter, the present invention will be described in detail through examples. However, these examples are for illustrative purposes only, and the scope of the present invention is not limited by these examples.

<Example>

1. Materials and methods

(One). Disclosure material

The species of species used in this experiment was Quercus myrsinifolia Blume), Quercus glauca Thunb, Quercus acuta Thunb), Quercus gilva ), Quercus phillyraeoides , and Quercus salicina Blume seeds were collected for each region.

Acorn collection period was from September to October, and acorns of thorns, thorns, red thorns, red thorns, and red thorns were collected from Jinju, and acorns of red thorns were collected from Jeju Island.

(2). Organic solvent extraction

Each species of acorns air-dried with a moisture content of 10% or less was immersed in 95% ethanol for 72 hours to extract. After the extraction solution was concentrated under reduced pressure, the crude extract obtained was freeze-dried. Extraction was repeated twice to obtain a sufficient amount of the extraction solution, and the obtained extraction solution yield was confirmed to be from 5.7% to 10.6%.

(3). Antioxidant activity evaluation

① DPPH activity

DPPH (1,1-diphenyl-2-picrylhydrazyl) assay was measured by the principle of free radical scavenging ability . 100 µl of the ethanol extract of each sample was added to 100 µl of 0.15 mM DPPH solution, and the reaction solution was thoroughly mixed, followed by reaction at room temperature for 10 minutes. Absorbance was measured at 517 nm using a UV-vis spectrophotometer to measure the amount of remaining DPPH. The antioxidant activity, free radical scavenging ability, was calculated by substituting into the following equation.

[Equation 1]

DPPH radical scavenging activity (%) = [1-(absorbance of sample at 517 nm ÷ absorbance of control at 517 nm)] × 100

② ABTS activity

The measurement of antioxidant activity using ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt) radical scavenging activity was measured by the ABTS cation decolorization assay, 7 mM ABTS and 2.4 mM potassium persulfate were mixed and left in the dark for more than 12 hours to form a cyan ABTS radical and stored in a refrigerator.The radical stock solution had an absorbance value of 0.70 at 450 nm ( ±0.02) was diluted with ethanol.

0.1 ml of each sample solution prepared by concentration was added to 0.9 ml of this solution, and then reacted at room temperature for 7 minutes, and then the change in absorbance of the reaction solution was measured at 450 nm. The degree of ABTS radical cation decolorization was expressed as the rate of decrease in absorbance of the sample solution added group and the non-added group.

(4). Antibacterial activity evaluation

The strain used in the antimicrobial activity test was Gram-negative Escherichia coli ), Pseudomonas aeruginosa and Gram-positive Staphylococcus aureus were used. At this time, the antimicrobial efficacy of each extract was measured by the paper disk method according to the Murray method (Murray PR et al., Manual of clinical microbiology (7th). Washington DC, ASM, pp 1527-1539, 1999).

The test strain was used for the main culture by increasing the activity in the optimal culture conditions and then incubating for 6 hours in an optimal medium such as nutrient broth to adjust the turbidity of the culture to Macfarland turbidity No. 0.5.

0.1 µl of the culture solution of each strain was evenly spread on an optimal medium such as nutrient agar medium. The concentration of each acorn extract was diluted in ethanol to be 10, 5, 1%, dispensed on a paper disk by 40 µl, and cultured at 37° C. for 24 hours, and then a microbial suppression ring was confirmed.

2. Results and Discussion

For the evaluation of antioxidant activity, extracts extracted with 95% ethanol were used. In general, phenolic compounds are known as major components showing antioxidant effects. In order to confirm the correlation between the phenol content and antioxidant power, the total phenol content of the sample is being analyzed after assays such as DPPH and ABTS are performed.

(One). DPPH assay

The degree of activity was expressed as EC ₅₀ (half maximal effective concentration; mg/ml) as the concentration of the antioxidant that reduces the DPPH concentration at the beginning of the reaction to 50%. As shown in FIG. 1, as a result, DPPH activity is also active by species. There is a difference in degree.

Referring to FIG. 1, red thorn> red oak> red thorn> red thorn>thorn> thorn The activity was highest in order, and the most important result is that the activity of the red thorn acorn extract was highest in the DPPH result. In the case of 6 species used in this experiment, EC ₅₀ results were shown at sample concentrations of 0.312 mg/ml to 0.019 mg/ml.

Although the DPPH assay is the simplest method for measuring antioxidant activity, there is an experimental limitation due to the mechanistic differences in hydrogen molecule transfer occurring in the reaction between antioxidants and peroxy radicals. Therefore, an additional ABTS assay was performed to clarify the result of the activity difference between species.

Since the evaluation of the activity is confirmed through the absorbance of UV in all additional experiments, an absorbance interference problem may occur in the case of a dark sample. In the case of this experiment, there is also a difference in degree, but since all six species contain their own color, the color of the sample does not interfere with the absorbance measurement, and the sample concentration within the DPPH activity range is 10 ㎎/㎖ ~ 0.625 ㎎/㎖. Performed.

(2). ABTS activity

Ascorbic acid, also known as vitamin C, is a representative reducing agent. Therefore, it is known as a major standard material used as a positive control for the evaluation of antioxidant reducing power.

In this example, ascorbic acid was used as a standard substance to compare the activity. As shown in Fig. 2, as a result of this evaluation, the activity was high in the order of red thorn> oak> close thorn> red thorn> thorn> thorn.

In general, the main substance of the antioxidant effect of the oak family is known as tannin, and in particular, it is known that the content of gallic acid is high, but studies on the tannin component present in the bramble acorn are considered to be carried out later.

(3). Antibacterial activity

As a result of measuring the antimicrobial activity of the ethanol extract of Thornwood against three species of food rot and food poisoning causative bacteria, it showed a somewhat higher inhibitory ring as shown in FIGS. 3 to 5. The extract that showed activity against all three bacteria ( E. coli , P. aeruginosa , S. aureus ) showed the highest effect of ethanol extract of red thorn acorn. For the remaining 5 species, differences in activity against bacteria were observed.

For S. aureus, in addition to red thorns, extracts of C. acorns and S. aureus showed antibacterial activity. The antimicrobial activity of extracts from P. aeruginosa , a gram-negative bacterium, was confirmed, but no activity was observed in other species. As a result, the activity of the acorn extract of the red thorn and the serrata was the highest in the antibacterial effect.

It is known that more than 80% of the causes of food poisoning are caused by microorganisms. In particular, as the food industry has developed into higher-order processing, the use of various additives and processing agents has increased, and thus far, it has mainly relied on chemical synthetic agents.

Compared to the fact that most natural products have a broader antimicrobial spectrum against Gram-positive bacteria than Gram-negative bacteria, the thorn extract derived from this study has antibacterial activity against Gram-negative bacteria, so it is likely to be used as an antibacterial material. I expect.

As described above, the present invention has been described with reference to the preferred embodiments of the present invention, but those skilled in the art of the present invention have the present invention within the scope not departing from the spirit and scope of the present invention described in the following claims. It will be appreciated that various modifications and changes can be made to the invention.

Antioxidant and antibacterial composition comprising the red thorn acorn extract of the present invention as an active ingredient, the concentration of the red thorn acorn extract was 0.312 mg/ml ~ 0.019 mg/ml, E. coli, Pseudomonas aeruginosa and Since it is possible to provide an antioxidant and antibacterial composition having antibacterial activity against bacteria such as Staphylococcus aureus, it can be usefully applied to the technical field to which the present invention pertains.

Claims (7)

Antioxidant and antibacterial composition comprising red thorn acorn extract as an active ingredient. The antioxidant and antibacterial composition of claim 1, wherein the red thorn acorn extract is an ethanol extract of red thorn acorn. The composition for antioxidant and antibacterial according to claim 1, wherein the concentration of the red thorn acorn extract is 0.312 mg/ml to 0.019 mg/ml. The method of claim 1, wherein the red thorn acorn extract is Escherichia coli ), Pseudomonas aeruginosa and Staphylococcus aureus ( Staphylococcus aureus ) Antioxidant and antibacterial composition, characterized in that it has antibacterial activity against one or more bacteria selected from the group consisting of. The antioxidant and antibacterial composition according to any one of claims 1 to 4, wherein the composition is a health functional food composition. The antioxidant and antibacterial composition according to any one of claims 1 to 4, wherein the composition is a pharmaceutical composition. The composition for antioxidant and antibacterial according to any one of claims 1 to 4, wherein the composition is a cosmetic composition.

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