KR20200119099A - Stable composition for controlling pests and method for controlling pests by using the same - Google Patents

Abstract

The present invention relates to a composition for controlling pests having stability against high temperature and moisture, and to a method for controlling pests using the same and, more specifically, to a composition for controlling pests, which uses silicone oil for the formulation of an oil base, and eliminates high temperature and moisture stability problems when stored using molecular sieves to control the amount of moisture in the formulation, and to a method for controlling pests using the same.

Description

Composition for controlling pests having stability and method for controlling pests using same {Stable composition for controlling pests and method for controlling pests by using the same}

The present invention relates to a composition for controlling pests having excellent stability against high temperature or moisture, and a method for controlling pests using the same.

In order to control pests and pests that damage agricultural crops, the use of chemical pesticides has increased further and the spraying area has become wider as the scale of cultivated crops increases with the development of agricultural technology. The emergence of organic synthetic pesticides after World War II marked a remarkable turning point in two aspects: the increase of agricultural crops through the control of agricultural pests and the improvement of human health through the control of sanitary pests. The amount of pesticides used for pest control in Korea is increasing every year, of which organophosphorus and carbamates account for about 90% of the total consumption.

However, due to decades of continuous use and abuse of these existing organic synthetic pesticides, various side effects such as emergence of resistant pests, human animal toxicity, biological control disturbance by natural enemies, residual poisons and environmental pollution have been caused. The use of synthetic pesticides is gradually being restricted as interest in the side effects and environmental impacts of the drug increase. Accordingly, biological control is being actively studied as a part of the development of next-generation pesticides that can replace synthetic pesticides, but biological control is an obstacle to commercialization because it is not easy to handle using microorganisms, natural enemies, or natural extracts.

In this regard, microbial crop protection agents that biologically control insect pathogenic fungi (fungi) have the advantage of minimizing resistance and environmental pollution by using fungal spores, unlike the chemical pesticides described above, but it is an important matter in commercialization. Studies on mass production and stabilized formulation are still insufficient. In particular, in order to control pests such as thrips, which absorb nutrients by absorbing the juice of plant leaves and reduce the value of feed, insect pathogenic fungi ( Beauveria bassiana ) are currently sold in granular form. However, formulations using these insect pathogenic fungi should be prepared by separating the spores produced through solid culture from the culture and mixing them with water in the form of a liquid formulation and a wettable powder for spray application. However, when mixed with water, spores are 1 It dies within a week and is somewhat stable at room temperature in the powder state, but there is a disadvantage that all die within a week when exposed to high temperatures (50°C or higher). It is common for crop protection agents to be exposed to temperatures above 40℃ during distribution.

Due to this problem, conventional insect pathogenic fungal preparations have many problems in physical properties and are inconvenient to use, and the expiration date is 1 year, so it is difficult to export and sell to other countries, so if storage stability is improved, a large ripple effect is expected.

In this regard, in Korean Patent Publication No. 10-2016-0084968, the strain of Boberia bassiana JEF007, an insect pathogenic fungus having an insect pathogenicity and excellent sporulation ability, and a liquid formulation for controlling agricultural pests using the same, and the strain using a filterable bag It discloses a method of manufacturing a liquid formulation containing, but does not mention a matter on storage stability. In addition, Korean Patent Publication No. 10-2008-0026101 relates to the stabilization of fungal substances, as a composition comprising a fungal substance, a solid substrate, and a biopolymer composition, using a solid substrate such as rice grains. It has been disclosed to reduce the loss of fungal substances due to fine powder formation since there is no need to separate the substrate from the fungi, but it does not disclose changing the composition of the pesticide formulation for storage stability due to high temperature or moisture.

Therefore, there is an urgent need for the development of a new pest control formulation that is harmless to the human body, is environmentally friendly, is easy to handle, and has excellent storage stability against high temperature and moisture.

Korean Patent Publication No. 10-2016-0084968 Korean Patent Publication No. 10-2008-0026101

Under this background, the present inventors have repeatedly studied in order to solve the stability problem that pathogenic fungi spores are inhibited by various factors such as moisture or high temperature. When combining silicone oil and molecular sieve with pathogenic fungi raw agents, moisture or By remarkably reducing the problem of reducing the stability of pathogenic fungi formulations due to high temperatures, it was confirmed that a composition for controlling pests and a method for controlling pests having excellent stability can be provided, thereby completing the present invention.

For example, there is provided a composition for controlling pests, including pathogenic fungi or spores thereof, silicone oil, and molecular sieves.

In another example, a pest control method is provided, comprising the step of applying the composition for controlling pests to pests, habitats of pests, or crops susceptible to pests.

As another example, there is provided a method for stabilizing a composition for controlling pests comprising the step of mixing and storing pathogenic fungi or spores thereof, silicone oil and molecular sieves.

In one aspect, the present invention relates to a composition for controlling pests, including insect pathogenic fungi or spores thereof, silicone oil and molecular sieve as an oily solvent.

As used herein, "pathogenic fungi" refers to fungi that cause disease in organisms and mainly proliferate through spores. The "entomopathogenic fungi", which is a preferred example of the pathogenic fungi to which the present invention is applicable, mainly belongs to imperfect fungi and conjugate fungi, taxonomically, and refers to fungi having pest control activity. Insect pathogenic fungi are different from insect pathogenic bacteria or viruses that are infected through host feeding, after fungal spores adhere to the outer shell of host insects and germinate, then pass through the cuticle layer or germinated from the spores with an invasion tool. It directly passes through the cuticle layer and invades the inside of the insect. At this time, the physical action and enzyme actions such as chitinase, protease, lipase, and esterase occur together. Fungi that invade the insect's body can be used as insect quarantine insecticides because they secrete toxic substances while proliferating in the insect's body to block the insect's immune function and kill host insects.

More than 700 species of insect pathogenic fungi are currently reported, and any one having an insecticidal effect on insects by generating spores can be used without limitation. For example, the beam Beria (Beauveria) in director Liao (Isaria), A Greca nisil Solarium (Lecanicillium) in ah (Nomuraea), A celecoxib Sat tree glutamicum (Celletotrichum) genus Fusarium (Fusarium) to Nomura in blood Saratov Tora (Phytophthora), A's Klee Loti California (Sclerotinia), Metairie Clear (Metarhizium), A par indeed My process (Paecilomyces), A tying yarn Solarium (Verticillium) genus Trichoderma (Trichoderma), A heosyu telra (Hirsutella ), Aspergillus , Aschersonia , or The fungi of the genus Metarhizium can be exemplified. Specifically, the correction Beria Bridge Ana (Beauveria bassiana), Metairie Clear No Cattle Plymouth Oh (Metarhizium anisopliae), Bertie Room Solarium Recaro you (Verticillium lecanii), ASCII Sonia Alessio This display (Aschersonia aleyrodis) rilreyiyi or to Nomura (Nomuraea rileyi ) Can be illustrated. The boberia bassiana used in an example of the present application is a strain having excellent insecticidal effect against saw-legged ants, yellow flower thrips, and the like.

These insect pathogenic fungi or spores thereof may be appropriately included in an amount capable of exhibiting a pest control effect, but preferably may be included in an amount of 1 to 90% by weight based on the total weight of the composition for controlling pests, and more specifically, 1 to 30% by weight, 5 to 20% by weight, more specifically 7 to 15% by weight, and most specifically 8 to 10% by weight, but is not limited thereto.

Spores are germ cells produced by fungi for asexual reproduction, and can develop into adults without forming a zygote, and preferably include conidia. The composition for controlling pests of the present application includes insect pathogenic fungi or spores thereof, which is understood to include spores, hyphae, mycelium, fruiting bodies, spores or their equivalents. When the spores touch the insect's shell, a germination tube is created from the spore, and the germination tube grows until the nutrients in the spore are depleted, and begins to penetrate the cuticle layer of the insect, and the germination tube penetrates the enzyme that dissolves the cuticle layer. Secrete.

In the present invention, in particular, in order to solve the stability problem due to high temperature or moisture, it is characterized in that it contains a molecular sieve capable of absorbing moisture as an additive. These molecular sieves can control the amount of moisture in the composition for controlling pests to a level that can maximize stability.

The "molecular sieve" means a porous solid material having uniform or different sized pores, and typically has a property of selectively adsorbing molecules that can enter the pores, and has various sizes and shapes, so that it can be used in various technical fields. It is applied, and inorganic substances such as zeolite, carbon molecular sieves, and the like can be exemplified. The shape and shape of the pores of the molecular sieve can be varied in a spherical shape, a channel shape, a slit shape, a hexagonal shape, and the like, and the molecular sieve itself can have various shapes and particle diameters such as a powder shape, a rod shape, a sphere shape, a tube shape, or a cylinder shape. . In addition, it may have a water absorption amount of 0.18 to 0.25 g/g, but is not limited thereto. As an example, the molecular sieve may be zeolite.

Zeolite, an example of the molecular sieve, is a crystalline aluminosilicate in which a plurality of pores are regularly formed. Zeolite is an inorganic polymer material formed by three-dimensionally connecting silicon (Si) and aluminum (Al) through an oxygen atom, and has various shapes and sizes of 3 Å to 100 Å (0.3 nm to 10 nm) depending on the type. It is structurally characterized by having a nanopore, and about 35 species are currently known. Among them, zeolite having a pore size of 3 to 5 Å is preferred, for example, zeolite 3A having a pore size of about 3 Å, zeolite 4A having a pore size of about 4 Å, or zeolite 5A having a pore size of about 5 Å are suitable for the present application. Can be illustrated. Zeolite can be used regardless of shape or diameter, such as rod-shaped, spherical or powder, and may have a diameter of, for example, 1.5 to 5.0 mm, and it is preferable to have a water absorption amount of 0.18 to 0.25 g/g, It is not limited thereto.

In the present application, the molecular sieve having a pore size of 3 to 5 Å lowers the moisture content in the composition, thereby contributing to the long-term stability of the composition for controlling pests. In particular, in the case of high temperature, it was confirmed that the molecular sieve of the present application effectively absorbs moisture in an environment where the influence of moisture is greater than that of room temperature to ensure stability, thereby maintaining stability even at high temperatures. If the pore diameter is less than 3 Å, moisture is not sufficiently absorbed, and if the pore diameter exceeds 5 Å, the proportion of other substances absorbed as well as moisture increases, resulting in high temperature conditions and long processing time required during regeneration. Have.

In a specific embodiment of the present application, zeolite 4A was used as a molecular sieve for the preparation of the pest control agent according to the present invention, but the scope of the present invention is not limited thereto.

In addition, the molecular sieve may be included in 1 to 50% by weight based on the total weight of the pest control composition, more specifically 1 to 30% by weight, 3 to 20% by weight, and most specifically 4 to 10% by weight It may be, but is not limited thereto. However, if it is less than the above range, there may be a problem in that sufficient moisture cannot be adsorbed.

In addition, the amount of moisture in the composition for controlling pests including the molecular sieve can be maintained at a level that can maximize stability, for example, 1% by weight or less, and more specifically 0.5% by weight or less, 0.3% by weight or less, Most specifically, it can be adjusted to a level of 0.1% by weight or less, but is not limited thereto.

Further, the composition for controlling pests according to the present invention is characterized in that it contains silicone oil as an oily solvent. Silicone oil has little change in viscosity over a wide temperature range, has excellent heat and cold resistance, is colorless, transparent, nonvolatile, and harmless to the human body, so it can maintain the stability of the pest control composition better than other oily solvents. To be. Preferably, the content of moisture in the silicone oil is characterized in that 0.1% by weight or less.

In addition, with respect to the total weight of the composition for controlling pests, the silicone oil may contain 30 to 95% by weight, more specifically 60 to 90% by weight, and most specifically 80 to 87% by weight, but limited thereto It does not become. Further, the silicone oil may be selected to have an appropriate viscosity as needed, but preferably may have a viscosity of 0.1 to 1000 cP at a temperature of 25°C, more specifically 0.5 to 500 cP, and most specifically May represent a viscosity of 0.5 to 200 cP, but is not limited thereto. In a specific embodiment, in the pesticide composition according to the present invention, dimethyl silicone oils KF96-50cs (50 cP viscosity) and KF96-100cs (100 cP viscosity) were used as silicone oil, but the scope of the present invention is not limited thereto. .

The composition for controlling pests of the present invention may also contain a surfactant. Surfactants are amphiphilic substances having both a hydrophilic molecular group and a lipophilic molecular group in a molecule, and have cleaning power, dispersing power, emulsifying power, solubilizing power, wetting power, sterilizing power, foaming power and/or penetrating power, and for controlling pests according to the present invention. Pathogenic fungi or spores in the composition may act to hydrate, suspend or disperse to effectively exert a pest control effect.

Surfactants include, by way of non-limiting examples, hydrophilic surfactants, lipophilic surfactants, or mixtures thereof. For example, the hydrophilic lipophilic balance (HLB) value of the surfactant may be about 1 to 20, or about 1 to 17. In one embodiment, the HLB of the surfactant may be 1 to 7, or 10 to 17, or 10 to 20. In another embodiment, the HLB of the surfactant may be 1 or more to less than 7, 10 to less than 17, or 10 to less than 20. The HLB range exemplifies a suitable range in which a surfactant can effectively dissolve in an oily solvent and exhibit a surfactant effect, but the scope of the present invention is not limited thereto. HLB is an index indicating the balance between hydrophilicity and lipophilicity (hydrophobicity) of a surfactant, and it is understood that the greater the HLB, the greater the proportion of hydrophilicity, and the smaller the HLB, the greater the proportion of lipophilic (hydrophobicity). The surfactant may also be nonionic, anionic, cationic, or zwitterionic (or amphoteric).

Examples of cationic surfactants usable herein include DTAB (Dodecyltrimethylammonium bromide) or CTAB (Cetyltrimethylammonium bromide), and examples of anionic surfactants include DSS (dioctyl sulfosuccinate sodium), SDS (sodium dodecyl sulfate), LDS (lithium dodecyl sulfate), sodium dodecyl benzene sulfonate (SDBS), sodium dodecylsulfonate (SDSA), and the like. Examples of amphoteric surfactants include lecithin, and examples of nonionic surfactants include La. Uryl PEG/PPG-18/18 Methicone, Cetyl Diglyceryl Tris(Trimethylsiloxy)silylethyl Dimethicone, PEG-9 Dimethicone (PEG-9 dimethicone), PEG-10 dimethicone, Sorbitan monooleate, Octylphenol Ethoxylate, polyethylene glycol, alkyl polyglucoside, silane poly Alkylene oxide (copolymer), nonylphenol ethoxylate, tristyrylphenol ethoxylate, polyoxyethylene alkyl ether, polyoxyethylene alkylphenyl ether, alkylallyl formaldehyde condensed polyoxyethylene ether, polyoxyethylene poly Oxypropylene block polymer, polyoxyethylene polyoxypropylene alkyl ether, polyoxyethylene ether of glycerin ester, polyoxyethylene ether of sorbitan ester, polyoxyethylene ether of sorbitol ester, polyethylene glycol fatty acid ester, glycerin ester, polyglycerin ester , Sorbitan ester, propylene glycol ester, sucrose ester, aliphatic Acid alkanol amide, polyoxyethylene fatty acid amide, alcohol ethoxylate, amine ethoxylate, glucoside, glucamide, polyethylene glycol, poly(ethylene glycol-co-propylene glycol), cetyl alcohol, stearyl alcohol, cetoste Aryl alcohol, oleyl alcohol, octaethylene glycol monododecyl ether, pentaethylene glycol monododecyl ether, polyoxypropylene glycol alkyl ether, decyl glucoside, lauryl glucoside, octyl glucoside, polyoxyethylene glycol octylphenol ether, nonoxy Nol-9, glycerol alkyl ester, glyceryl laurate, polyoxyethylene glycol sorbitan alkyl ester, polysorbate, sorbitan alkyl ester, span, cocamide MEA, cocamide DEA, dodecyldimethylamine oxide , A block copolymer of polyethylene glycol, polypropylene glycol, and a silane polyalkylene oxide (copolymer), or polyoxyethylene alkyl amide, for example, Triton (registered trademark) X-114, X-102, X-100, X-45, X-15, BG-10 or CG-119, or BRIJ (registered trademark) polyoxyethylene-4-lauryl ether (brij30), polyoxyethylene-23-lauryl ether (brij35 ), polyoxyethylene-2-cetyl ether (brij52), polyoxyethylene-10-cetyl ether (brij56), polyoxyethylene-20-cetyl ether (brij58), polyoxyethylene-2-stearyl ether (brij72) , Polyoxyethylene-10-stearyl ether (brij76), polyoxyethylene-2-oleyl ether (brij93) or polyoxyethylene-10-oleyl ether (brij97), PEG400 dioleate (PEG-8 dioleate) Such as may be illustrated, but is not limited thereto.

In one embodiment of the present application, the surfactant is one selected from the group consisting of lauryl PEG/PPG-18/18 methicone, cetyl diglyceryl tris(trimethylsiloxy)silylethyl dimethicone, and PEG-9 dimethicone. It may be abnormal, but is not limited thereto.

In a specific embodiment, the composition for controlling pests according to the present invention includes Dow 5200 (lauryl PEG/PPG-18/18 methicone), Dow 5600 (cetyl diglyceryl tris (trimethylsiloxy) silylethyl dimethicone) and Although KF-6015 (PEG-9 dimethicone) was used, the scope of the present invention is not limited thereto.

In a preferred embodiment, the surfactant may contain 0.001 to 90% by weight, more specifically 0.001 to 50% by weight, 0.05 to 20% by weight, 0.05 to 4% by weight, and most, based on the total weight of the composition for controlling pests. Specifically, it may be included in 0.05 to 1.0% by weight, but is not limited thereto. However, if it is less than the above range, there may be a problem that the surfactant effect does not appear sufficiently, and if it exceeds the above range, there may be a problem that the surfactant does not dissolve in the solvent and precipitation or precipitation occurs.

The composition for controlling pests according to the present invention may be prepared according to a known method for preparing a composition for controlling pests. For example, it can be prepared by combining pathogenic fungi or spores thereof, silicone oil and molecular sieve, and optionally a surfactant. Pathogenic fungi or spores thereof can be pulverized to have an appropriate particle size if necessary, and after preparing a liquid sample by adding a surfactant and a molecular sieve to an oily solvent, the pulverized pathogenic spores are mixed with the pests according to the present invention. A composition for controlling can be prepared. The composition for controlling pests according to the present invention may be formulated in various forms, but a liquid concentrate form capable of spraying is preferred.

The composition for controlling pests according to the present invention exhibits excellent stability not only at room temperature but also at high temperature. Specifically, the composition for controlling pests according to the present invention is at 50°C, or 54°C or higher, for example, at 50°C to 60°C, 50°C to 55°C, or 50°C to 54°C, the insect pathogenic fungus or More than 1.0% of its spores can survive for over a week. In addition, at room temperature, or 50°C, or 54°C or more, for example, 50°C to 60°C, 50°C to 55°C, or 50°C to 54°C, for 1 week or more, preferably 5 to 6 weeks. It is characterized by maintaining pathogenicity for more than a week. In addition, at room temperature, or at 50°C, or at 54°C or higher, for example, at 50°C to 60°C, 50°C to 55°C, or at 50°C to 54°C, preferably at least 6 weeks effective bacteria count or more, specifically It is characterized by maintaining the number of bacteria of 1.0E+05 CFU/mL or more.

In another aspect, the present invention comprises a silicone oil as an oily solvent and a molecular sieve as an additive, a pathogenic fungus or spores thereof, and a composition for controlling pests comprising a surfactant as a dispersant, so that the survival rate of the insect pathogenic fungi or spores thereof It relates to how to increase. Preferably, the silicone oil and molecular sieve are used to increase the survival rate of insect pathogenic fungi or spores thereof compared to a state in which the moisture content is high by maintaining a sufficiently low moisture content for a long period of time. It features.

In another aspect, the present invention relates to a method for stabilizing a composition for controlling pests, comprising the step of mixing and storing pathogenic fungi or spores, silicone oil, and molecular sieves thereof.

In another aspect, the present invention relates to a method for controlling pests by applying the composition for controlling pests according to the present invention to pests, habitats of pests, or crops susceptible to pests.

The composition of the present invention may be used in a method for controlling pests by applying the composition for controlling pests according to the present invention to pests, habitats of pests, or crops susceptible to pests.

Examples of pests controllable by the composition of this embodiment include arthropods such as insects and ticks, especially harmful arthropods such as harmful insects and harmful mites, and the following can be exemplified:

- half bandwagon (Hemiptera): myeolguryu (Delphacidae), for example aemyeolgu (Laodelphax striatellus), Brown Planthopper (Nilaparvata lugens) and huindeung myeolgu (Sogatella furcifera); Cicadas (Deltocephalidae), such as Nephotettix cincticeps and Nephotettix virescens ; Jinditmulryu (Aphididae), for example, cotton aphid (Aphis gossypii), Green Peach Aphid (Myzus persicae), cabbage powder aphid (Brevicoryne brassicae), potato beard aphid (Macrosiphum euphorbiae), bush clover beard aphid (Aulacorthum solani), millet border aphid (Rhopalosiphum padi ) and tangerine aphid ( Toxoptera citricidus ); Pentatomidae, such as Nezara antennata , Saw-legged ant, Riptortus clavetus , Leptocorisa chinensis , Eysarcoris parvus , Halyomorpha mista and Halyomorpha mista ( Lyuslineolaris ); Aleyrodidae , such as Trialeurodes vaporariorum , Bemisiatabaci , and Bemisia argentifolii ; Snapworms ( Coccidae ), such as California red snapworm ( Aonidiella aurantii ), San Jose snapworm ( Comstockaspis perniciosa ), arrowhead bug ( Unaspiscitri ), ruby snapworm ( Ceroplastes rubens ), and iseria snapworm ( Icerya purchasi ) ; Shield bugs (Tingidae); Psyllidae , etc.;

-Lepidoptera : Pyralidae , such as Chilo suppressalis , Yellow rice beetle ( Tryporyzaincertulas ), Cnaphalocrocrocis medinalis , Cotton moth ( Notarchaderogata ), Hwaranggok moth ( Plodia interpunctella ) Moth ( Ostrinia furnacalis ), European lighted moth ( Ostrinia nubilaris ), Chinese cabbage sprout moth ( Hellula undalis ) and grass insect moth ( Pediasiateterrellus ); Chestnut moths ( Noctuidae ), such as tobacco moth ( Spodoptera litura ), green chestnut moth ( Spodoptera exigua ), Pseudaletia separata , thief moth ( Mamestra brassicae ), roundup seminar ( Agrotis ipsilon ), trapezoidal chestnut Butterfly ( Plusianigrisigna ), Thoricoplusia spp., tobacco chestnut moth ( Heliothis spp.) and tobacco moth ( Helicoverpa spp.); White butterfly ( Pieridae ), such as Chinese cabbage white butterfly ( Pierisrapae ); Tortricidae , for example, Adoxophyes spp., Peach moth ( Grapholitamolesta ), Bean moth ( Leguminivora glycinivorella ), Red bean moth ( Matsumuraeses azukivora ), Apple apex moth ( Adoxophyesorana fasciata ) pattern ipmalyi moth (Adoxophyes honmai), tea leaves, horse moth (Homona magnanima), geommo pattern ipmalyi moth (Archipsfuscocupreanus) and code Lin moth (Cydia pomonella); Fine moths ( Gracillariidae ), such as Camellia theivora ( Caloptilia theivora ) and Apple oyster moth ( Phyllonorycter ringoniella ); Heart food moth ( Carposinidae ), such as peach heart food moth ( Carposina niponensis ); Oyster moth ( Lyonetiidae ), such as silver pattern oyster moth ( Lyonetia spp.); Poisonous moths ( Lymantriidae ), such as gypsy moths ( Lymantria spp.) and poisonous moths ( Euproctis spp.); House moth ( Yponomeutidae ), such as Chinese cabbage moth ( Plutella xylostella ); Geelechiidae , such as Pectinophora gossypiella and Phthorimaea operculella ; Fire moth ( Arctiidae ), such as American white fire moth ( Hyphantria cunea ); And grain moths ( Tineidae ), such as Tinea translucens ;

-Thysanoptera: yellow flower thrips ( Frankliniella occidentalis ), cucumber thrips ( Thrips palmi ), convex thrips ( Scirtothrips dorsalis ), thrips ( Thripstabaci ), Taiwanese thrips ( Frankliniella intonsa ) and tobacco thrips Worms ( Frankliniella fusca ), etc.;

-Diptera: oyster flies ( Agromyzidae ), such as oyster flies ( Hylemyaantiqua ), sedge flies ( Hylemyaplatura ), rice leaf oyster flies ( Agromyza oryzae ), rice leaf oyster flies ( Hydrellia griseola ), rice yellow oyster flies ( Chlorops oryzae ) , American leaf oyster fly ( Liriomyza trifolii ); Melon fly ( Dacus cucurbitae ) and Mediterranean fruit fly ( Ceratitis capitata ), etc.;

- second bandwagon (Coleoptera): 28-spotted ladybird (Epilachna vigintioctopunctata), cucumber beetle (Aulacophora femoralis), flea beetle (Phyllotreta striolata), rice beetle (Oulema oryzae), rice root weevil (Echinocnemus squameus), byeomul weevil (Lissorhoptrus oryzophilus ), Cotton Weevil ( Anthonomus grandis ), Red Weevil ( Callosobruchus chinensis ), Hunting Weevil ( Sphenophorus venatus ), Japanese Bean Beetle ( Popillia japonica ), Copper Beetle ( Anomala cuprea ), Corn Leaf Beetle ( Diabrotica spp.), Colorado Potato Leaf Beetle ( . Leptinotarsa decemlineata), skipjack acids (Agriotes spp), etc. and the cigarette beetle (Lasioderma serricorne);

-Grasshoppers (Orthoptera): land dog ( Gryllotalpa africana ), rice grasshopper ( Oxya yezoensis ) and rice grasshopper ( Oxya japonica ), etc.;

-Hymenoptera : leafless bee ( Athalia rosae ), scissor ants (worker ants) ( Acromyrmex spp.) and fire ants ( Solenopsis spp.), etc.;

-Mites (Acarina): mites (Tetranychidae), such as spotted mites ( Tetranychus urticae ), tangerine mites ( Panonychus citri ) and tree mites ( Oligonychus spp.); Hawke Mites (Eriophyidae), for example gyulnok mite (Aculops pelekassi); Dust mites ( Tarsonemidae ), such as tea dust mites ( Polyphagotarsonemus latus ); Earth mite ( Tenuipalpidae ); Chire Mites (tuckerellidae); Powdery mites ( Acaridae ), such as long hairy mites ( Tyrophagus putrescentiae ); Dust mites ( Pyroglyphidae ), such as large leg dust mites ( Dermatophagoides farinae ) and vertical pattern dust mites ( Dermatophagoides ptrenyssnus ); Claw mites ( Cheyletidae ), such as short claw mites ( Cheyletus eruditus ), claw mites ( Cheyletus malaccensis ) and Keyletus moorei ( Cheyletus moorei );

-Nematodes : rice leaf nematodes ( Aphelenchoides besseyi ) and strawberry sub-nematodes ( Nothotylenchusacris ), etc.

One example of a method for controlling pests using the composition of the present application is to spray the composition of the present application on a pest or pest habitat of a treatment target. "Pest habitat" means the area where the pest actually lives, as well as at least the area where the pest passes or moves. In addition, it means an area in which a habitat or passage of a pest is assumed, as well as an area in which the pest is preferably eliminated.

Examples of plants to which the compositions or methods herein can be applied are as follows:

-Crops: corn, rice, wheat, barley, rye, oats, sorghum, cotton, soybean, peanut, buckwheat, sugar beet, rapeseed, sunflower, sugar cane and tobacco;

-Vegetables: Eggplant and vegetables (eggplant, tomato, bell pepper, pepper, potato, etc.), gourd and vegetables (cucumber, pumpkin, zucchini, watermelon, melon, etc.), cruciferous vegetables (wagered radish, turnip, horseradish, kohlrabi, cabbage, etc.) Cabbage, black mustard, broccoli, cauliflower, rapeseed, etc.), asteraceae vegetables (burdock, garland chrysanthemum, artichoke, lettuce, etc.), lilies and vegetables (green onion, onion, garlic, asparagus, etc.), parsley (carrot, parsley, celery, etc.) , Parsnip, etc.), pollock vegetables (spinach, chard, etc.), lamiaceae vegetables (perilla, mint, basil, etc.), strawberries, sweet potatoes, yams, taro, etc.;

-Fruit trees: causal fruits (apple, pear, Japanese pear, Chinese quince, quince, etc.), stone fruits (peach, plum, nectarine, loquat, cherry, apricot, prune, etc.), citrus fruits (Wenzhou mandarin, orange, lemon, lime, etc.) Grapefruit), nuts (chestnuts, walnuts, hazelnuts, almonds, pistachios, cashews, macadamia nuts, etc.), berries (blueberries, cranberries, blackberries, raspberries, etc.), grapes, persimmons, olives, loquats, bananas, coffee, dates , Coconut, oil palm, etc.;

-Trees other than fruit trees: green tea, mulberry, flower tree (azalea, camellia, hydrangea, baby camellia, Japanese star anise, cherry tree, tulip tree, lagerstroemia tree, gypsy tree, etc.), street trees (ash, birch Tree, Dogwood, Eucalyptus, Ginkgo, Lilac, Maple, Oak, Poplar, Bacterial Tree, Taiwan Style Tree, Plane Tree, Zelkova, Japanese Cypress, Fir, Hem-Fir, Juniper, Pine, Spruce, Yew Tree , spruce, elm, Aesculus turbinata, etc.), sanhosu (coral tree), or Hansong (podocarpus), cedar, cypress, croton (croton), Euonymus japonicus (Euonymusjaponicus), Photinia glabra (Photiniaglabra), etc .;

-Lawn : Grass (zoysiagrass, Zoysiamatrella , etc.), umbrella grass ( Cynodondactylon, etc.), top (Bent grass) ( Agrostisalba ), creeping bent grass, highland bent grass, hiland bent), bluegrass (meadow grass, bird grass, etc.), fescue (tall fescue), chewings fescue, creeping red fescue red fescue, etc.), ryegrass (darnel, ryegrass, etc.), orchard grass, timothy grass, etc.;

-Others: flowers (rose, carnation, chrysanthemum, prairie gentian), gypsophila, gerbera, marigold, salvia, petunia, verbena, tulip , Aster, gentian, lily, pansy, cyclamen, orchid, lily of the valley (convallaria), lavender, stock, ornamental cabbage, primula, poinsettia, gladiolus ), cattleya, daisy, cymbidium, begonia, etc.), biofuel plants (Jatropha, safflower, camelina, switchgrass, silver grass ( Miscanthus), reed canary grass, giant reed, kenaf, cassava, willow, etc.), ornamental plants, etc.

The pest control composition of the present invention may be applied to plants or plant cultivation areas for pest control. Plants as used herein include stems or leaves of plants, flowers of plants, fruits of plants, seeds of plants, and the like. Examples of application methods include application to stems and leaves of plants, such as foliage application; Application to the seeds of plants; And applications to areas where plants are cultivated, such as soil applications and submerged applications.

The composition for controlling pests according to the present invention uses silicone oil as an oily solvent and molecular sieve as an additive, excellently improving the stability problem caused by high temperature and moisture, which is a problem in conventional pest control compositions, It is very effective by reducing the decrease in potency due to temperature and moisture during storage.

Hereinafter, the present invention will be described in detail through Examples and Experimental Examples, but the following Examples and Experimental Examples are only illustrative of the present invention, and the scope of the present invention is not construed as being limited to the following Examples and Experimental Examples. .

Preparation of ingredients

As the pathogenic spore strain, Beauveria bassiana ERL836 (KCCM11506P) was used. For the cultivation of the fungus, solid culture was performed using grain as a medium material. For this, first, spores of Boberia bassiana ERL836 stored at -70°C were spread on potato agar medium (Potato Dextrose Agar), and then cultured at 25°C for 7 days. Spores were recovered, inoculated into millet, and cultured for 1 week to perform seed culture. For this culture, millet was immersed in water to sufficiently contain moisture, and then put in a steamer and steamed for 10 minutes or more at a high temperature of 100°C or higher. The increased millet was cooled at room temperature, and the seed culture was inoculated and mixed. The mixture was incubated at 25° C. for 5 days or longer, and the culture was dried at low temperature to recover spores. When the number of spores in the original formulation was 1 X 10 ⁹ CFU/g or more, it was taken as normal culture.

Silicone oil (KF96-50cs, KF96-100cs) and mineral oil (8042-47-5, Sigma) were used as oily solvents.

Dow 5200 (lauryl PEG/PPG-18/18 methicone, nonionic, HLB=6.8) as surfactant, Dow 5600 (cetyl diglyceryl tris(trimethylsiloxy)silylethyl dimethicone, nonionic, HLB=2 ), KF-6015 (PEG-9 dimethicone, nonionic, HLB=4.5), and PEG400 dioleate (PEG-8 dioleate, nonionic, HLB=8.3) were used.

As a molecular sieve, a zeolite 4A rod (pore size of about 4Å, diameter of about 1.6mm) (Daejeonghwa Geum, Korea) was used.

Experimental Example: Preparation and storage stability analysis of the composition for controlling pests according to the present invention

An example of a composition for controlling pests including silicone oil and molecular sieve according to the present invention was prepared according to the following method.

Test Methods:

i) Boberia bassiana ERL836, the original pathogenic spore, was uniformly crushed with a mortar.

ii) The original agent, oil, surfactant and molecular sieve samples according to the conditions of Table 1 were prepared.

Unit: mass ratio (wt%) Sample number Original title oil Surfactants Molecular sieve Example 1 9.1 50 cP silicone oil 85 Dow 5200 1.4 4.5 Example 2 9.1 50 cP silicone oil 85 Dow 5600 1.4 4.5 Example 3 9.1 50 cP silicone oil 85 KF-6015 1.4 4.5 Example 4 9.1 100 cP silicone oil 85 Dow 5200 1.4 4.5 Comparative Example 1 100 none 0 none 0 0 Comparative Example 2 9.1 Mineral oil 89.5 PEG400 dioleate 1.4 0

iii) Samples prepared according to the content ratio in Table 1 were prepared as follows.

-Examples 1, 2, 3 and 4: After 1 hour after mixing the oil and molecular sieve, a surfactant was added and vortexing was performed for 30 seconds. Thereafter, sterilization was performed at 80° C. for 30 minutes, and the original agent was added, followed by vortexing for 1 minute.

-Comparative Example 1: A 100% by weight sample of the original agent was prepared.

-Comparative Example 2: After adding a surfactant to the oil and vortexing for 30 seconds, sterilization was performed at 80°C for 30 minutes, and vortexing was added for 1 minute by adding the original agent.

iv) Each test sample was stored at room temperature (25°C) and 54°C, respectively.

v) Dilute 1 ml of sample samples in an aqueous solution (0.85 wt% NaCl and 0.27 wt% dioctyl sulfosuccinate sodium) for a total of 6 weeks at intervals of 1 week, and add vortexing for 1 minute to disperse. Then, it was spread on PDA (Tartaric acid) medium and cultured in an incubator at 25°C.

vi) The number of active spore colonies was counted (unit: CFU (Colony forming unit)/mL) through viable spore count.

The test results are shown in Table 2 below.

Storage temperature Week 1 Week 2 Week 3 Week 4 Week 5 Week 6 Example 1 Room temperature 6.0E+07 1.5E+07 3.7E+07 3.3E+07 3.9E+07 2.6E+07 54℃ 1.8E+07 4.9E+07 6.2E+06 7.5E+06 1.2E+06 3.7E+05 Example 2 Room temperature 2.2E+07 1.5E+07 4.4E+07 8.9E+07 2.7E+07 8.0E+07 54℃ 4.8E+07 4.7E+07 2.4E+08 2.0E+08 1.6E+08 9.9E+06 Example 3 Room temperature 5.0E+08 4.0E+08 2.7E+08 2.3E+08 2.3E+08 1.9E+08 54℃ 3.0E+08 1.7E+08 1.7E+08 1.5E+08 4.6E+07 1.6E+07 Example 4 Room temperature 5.7E+08 3.5E+08 7.9E+08 3.8E+08 4.1E+08 2.6E+08 54℃ 3.6E+08 3.4E+08 3.4E+08 2.2E+08 1.4E+08 5.9E+07 Comparative Example 1 Room temperature 6.4E+09 7.4E+09 1.0E+10 1.0E+10 9.7E+09 7.1E+09 54℃ Less than 1.0E+05 Less than 1.0E+05 Less than 1.0E+05 Less than 1.0E+05 Less than 1.0E+05 Less than 1.0E+05 Comparative Example 2 Room temperature 6.9E+08 5.8E+08 4.3E+08 3.8E+08 3.9E+08 3.7E+08 54℃ Less than 1.0E+05 Less than 1.0E+05 Less than 1.0E+05 Less than 1.0E+05 Less than 1.0E+05 Less than 1.0E+05

(*Unit: CFU (Colony forming unit)/mL)

As can be seen in Table 2 above,

-As in Comparative Example 1, the original drug itself showed a concentration of less than 1.0E+05 CFU/mL, which is the effective level of the drug from the first week at 54°C, indicating that there is no high temperature stability.

-In Comparative Example 2, even if the original agent was added to mineral oil, it was confirmed that there was no high temperature stability, showing a concentration of less than 1.0E+05 CFU/mL, which is an effective drug level from week 1 at 54°C.

-In Example 1, it was confirmed that the spore concentration similar to the room temperature condition was maintained for up to 4 weeks, and the high temperature stability up to 6 weeks was maintained above the effective level of the drug at a spore concentration of 3.7E+05 CFU/mL.

-In Example 2, it was confirmed that the high temperature stability up to 6 weeks using Dow 5600 as a surfactant was improved to a spore concentration of 9.9E+06 CFU/mL.

-In Example 3, using KF-6015 as a surfactant, it was confirmed that the high temperature stability up to 6 weeks was improved to a spore concentration of 1.6E+07 CFU/mL.

-In Example 4, when using a silicone oil (100 cP) having a higher viscosity than the silicone oil (viscosity 50 cP) conducted in Example 1, the high temperature stability up to 6 weeks was improved to a spore concentration of 5.9E+07 CFU/mL. Could be confirmed.

Claims (21)

Pathogenic fungi or spores thereof;
Silicone oil; And
Molecular sieve
Containing, pest control composition. The composition for controlling pests according to claim 1, wherein storage stability is improved at 50° C. or higher. The composition for controlling pests according to claim 1, wherein the pathogenic fungi or spores thereof maintain a number of bacteria of 1.0E+05 CFU/mL or more for 1 week or more at room temperature and 54°C. The method of claim 1, wherein the pathogenic microorganism strain or spore is the genus Beauveria , the genus Isaria , the genus Lecanicillium , the genus Nomuraea , and the celletotrichum . genus Fusarium (Fusarium) in blood Saratov Tora (Phytophthora), A's Klee Loti California (Sclerotinia), Metairie Clear (Metarhizium), A par indeed My process (Paecilomyces), A tying yarn Solarium (Verticillium) genus Trichoderma ( Trichoderma ) genus, Hersutella ( Hirsutella ) genus, Aspergillus ( Aspergillus ) genus, Aschersonia ( Aschersonia ) genus , Metarhizium (Metarrhizium ) a fungus selected from the genus (Metarrhizium ) genus and combinations thereof, pest control Dragon composition. The composition for controlling pests according to claim 1, wherein the pathogenic fungi or spores thereof are Boberia bassiana or spores thereof. The composition for controlling pests according to claim 1, wherein the pathogenic fungi or spores thereof are contained in an amount of 1 to 90% by weight based on the total weight of the composition. The composition for controlling pests according to claim 1, wherein the molecular sieve is a rod-shaped molecular sieve, a spherical molecular sieve, or a powder-shaped molecular sieve. The composition for controlling pests according to claim 1, wherein the molecular sieve is zeolite. The composition for controlling pests according to claim 1, wherein the molecular sieve absorbs moisture from 0.18 to 0.25 g/g. The composition for controlling pests according to claim 1, wherein the molecular sieve has a pore diameter of 3 to 5Å. The composition for controlling pests according to claim 1, wherein the molecular sieve is contained in an amount of 1 to 50% by weight, based on the total weight of the composition. According to claim 1, wherein the viscosity of the silicone oil is 0.1 to 1000 cP at 25 ℃, pest control composition. The composition for controlling pests of claim 1, wherein the silicone oil is contained in an amount of 30 to 95% by weight based on the total weight of the composition. The composition for controlling pests according to claim 1, wherein the composition for controlling pests further comprises a surfactant. The composition for controlling pests according to claim 14, wherein the surfactant has an HLB value of 1 to 17. The composition for controlling pests according to claim 14, wherein the surfactant has an HLB value of 1 to 7, or 10 to 17. The composition for controlling pests according to claim 14, wherein the surfactant is contained in an amount of 0.001 to 90% by weight based on the total weight of the composition. The method of claim 14, wherein the surfactant is one selected from the group consisting of lauryl PEG/PPG-18/18 methicone, cetyl diglyceryl tris(trimethylsiloxy)silylethyl dimethicone, and PEG-9 dimethicone. What is above, a composition for controlling pests. The composition for controlling pests according to claim 1, which is in the form of a concentrate for spraying. A method for controlling pests comprising applying the composition for controlling pests of any one of claims 1 to 19 to pests, habitats of pests, or crops susceptible to pests. Pathogenic fungi or spores thereof;
Silicone oil as an oily solvent; And
A method for stabilizing the composition for controlling pests of any one of claims 1 to 19, comprising the step of mixing and storing a molecular sieve.