ES2355457B1 - COMPOSITION AND METHOD TO CONTROL PESTS, MOWERS OR BOTH. - Google Patents

Abstract

The present invention provides a composition for controlling pests, mites or both pests and mites containing clothianidine and fosmet as active ingredients, a method for controlling pests, mites or both pests and mites that consists in applying effective amounts of clothianidine and phosmet to pests , the mites or both the pests and the mites or to the places where the pests inhabit or inhabit, the mites or both the pests and the mites, and a method to control pests, mites or both pests and mites that involves applying quantities Effective of clothianidine and fosmet to plants or their surroundings or to the soil in which plants grow. The composition of the present invention has a higher activity and the method of the present invention can effectively control pests, mites or both pests and mites.

Description

Composition and method to control pests, mites or both.

Field of the Invention

The present invention relates to a composition for controlling pests, mites or both pests and mites, and with a method of controlling pests, mites or both pests and mites.

Background of the invention

Until now, clothianidine and fosmet have been known as compounds with pesticidal and acaricidal activity (see, for example, The Pesticide Manual - Fourteenth Edition, published by BCPC, ISBN 1901396142, p. 209 and p. 828).

Summary of the Invention

The inventors of the present invention studied in depth and, as a result, saw that clothianidine and fosmet were used in combination to improve the control effect on pests, mites or both pests and mites. Thus, the present invention was completed.

The present invention provides:

[1] A composition for controlling pests, mites or both pests and mites, where the composition includes clothianidine and fosmet as active ingredients;

[2] The composition according to [1], where the weight ratio of clothianidine to phosmet is from 10: 1 to 1: 100;

[3] The composition according to [1], where the weight ratio of clothianidine to phosmet is 1: 2 to 1: 100;

[4] A method to control pests, mites or both pests and mites, which consists in applying effective amounts of clothianidine and phosmet to pests, mites or both pests and mites, or to places where pests, mites or both pests and mites;

[5] A method to control pests, mites or both pests and mites, which consists in applying effective amounts of clotianidine and phosmet to plants or their surroundings or to the soil in which plants are grown.

[6] The use of a combination of clothianidine and fosmet to control pests, mites or both pests and mites.

Detailed description of the preferred embodiments

Clothianidine and fosmet are known compounds and are described, for example, in The Pesticide Manual - Fourteenth Edition, published by BCPC, ISBN 1901396142, p. 209 and p. 828. Clothianidine and fosmet can be produced by known methods, which have commercial availability.

The weight ratio of clothianidine to fosmet in the pest, mite or pest control or mite control composition of the present invention is preferably 10: 1 to 1: 100 (= clothianidine: fosmet), more preferably 1 : 2 to 1: 100, although the weight ratio of clothianidine to fosmet may vary appropriately depending on the species of pests or mites to be controlled.

The composition of the present invention may simply be a mixture of clothianidine and phosmet. Normally, the composition of the present invention takes the form of a formulation, such as an oily solution, an emulsifiable concentrate, a suspension concentrate, a hydratable powder, a water dispersible granule, a powder, a granule, a microcapsule or a microgranule, which is obtained by mixing clothianidine and phosmet with an inert support and eventually adding a surfactant and other auxiliary agents for formulation to the mixture.

The total amount of clothianidine and phosmet contained in the composition of the present invention is usually 0.1 to 99% by weight, preferably 0.2 to 90% by weight, more preferably 1 to 80% by weight, by weight. of the composition. (Hereinafter, clotianidine and fosmet can be referred to collectively as "the present active ingredients").

The inert support used for the formulation includes a solid support and a liquid support.

Examples of the solid support include fine powder or granules of minerals, such as kaolin clay, attapulgite clay, bentonite, montmorillonite, acid clay, pyrophyte, talcum, diatomaceous earth and calcite; natural organic substances, such as corn cobs and nutshell flour; synthetic organic substances, such as urea; salts, such as calcium carbonate and ammonium sulfate; and synthetic inorganic substances, such as synthetic hydrated silicon oxide.

Examples of the liquid support include aromatic hydrocarbons, such as xylene, alkyl benzene and methylnaphthalene; alcohols, such as 2-propanol, ethylene glycol, propylene glycol and ethylene glycol monoethyl ether; ketones, such as acetone, cyclohexanone and isophorone; vegetable oils, such as soybean oil and cottonseed oil; aliphatic petroleum hydrocarbons; esters; dimethyl sulfoxide; acetonitrile; and water.

Examples of the surfactant include anionic surfactants, such as alkyl sulfates, alkylaryl sulphonates, dialkyl sulfosuccinates, polyoxyethylene alkylaryl ether phosphates, lignin sulphonate and naphthalene formaldehyde sulfonate polycondensates; nonionic surfactants, such as polyoxyethylene alkylaryl ethers, polyoxyethylene alkyl polyoxypropylene block copolymers and sorbitan esters and fatty acids; and cationic surfactants, such as alkyltrimethylammonium salts.

Examples of the other auxiliary agents for the formulation include water-soluble polymers, such as polyvinyl alcohol and polyvinylpyrrolidone; saccharides, such as gum arabic, alginic acid and a salt thereof, CMC (carboxymethyl cellulose) and xanthan gum; inorganic substances, such as magnesium and aluminum silicate and alumina sol; preservatives; dyes; and stabilizers, such as PAP (iso-propyl acid phosphate) and BHT (2,6-di-tert-butyl-4-methylphenol).

The composition of the present invention can protect plants as described below from damage due to pests or mites that eat or suck them.

Examples of pests on which the composition of the present invention has a controlling effect include:

Hemiptera:

Leafhoppers (Delphacidae), such as the small brown leafhopper (Laodelphax striatellus), the brown rice leafhopper (Nilaparvata lugens) and the white-leafed rice leafhopper (Sogatella furcifera), and chicharritas (Deltocephalidae), such as the rice chicharrite green (Nephotettix cincticeps) and green chicharrite (Nephotettix virescens); aphids (Aphididae), such as cotton aphid (Aphis gossypii), green peach aphid (Myzus persicae), cabbage aphid (Brevicoryne brassicae), potato aphid (Macrosiphum euphorbiae), Solanaceae aphid (Aulacorthum solani), the oat aphid (Rhopalosiphum padi) and the black citrus aphid (Toxoptera citricidus); Stink bugs (Pentatomidae), such as the green bug (Nezara antennata), the bean bug (Riptortus clavatus), the rice bug (Leptocorisa chinensis), the white spotted spiny bug (Eysarcoris parvus), the brown-brown bug (Halyomorpha mista) and the bed bug ligus (Lygus lineolaris); whiteflies (Aleyrodidae), such as the greenhouse whitefly (Trialeurodes vaporariorum), the sweet potato whitefly (Bemisia tabaci) and the silver leaf whitefly (Bemisia argentifolia); mealybugs (Coccoidea), such as the California red mealybug (Aonidiella aurantii), the San José mealybug (Comstockaspis perniciosa), the citrus mealybug (Unaspis citri), the red mealybug (Ceroplastes rubens), the ribbed mealybug (Icerya purchasi) and mealybugs (Pseudococcidae); lace bugs (Tingidae); psilids (Psyllidae), such as pear pear (Cacopsylla pyricola) and Asian citrus psyllid (Diaphorina citri), etc.

Lepidoptera:

Pyralids (Pyralidae), such as the rice borer (Chilo suppressalis), the yellow rice stem borer (Tryporyza incertulas), the rice leaf winder (Cnaphalocrocis medinalis), the cotton leaf winder (Notarcha derogata ), the Indian flour moth (Plodia interpunctella), the eastern corn drill (Ostrinia furnacalis), the European corn drill (Ostrinia nubilalis), the cabbage worm (Hellula undalis) and the grass caterpillar meadows (Pediasia teterrellus); nocturnal butterflies (Noctuidae), such as the gray tobacco worm (Spodoptera litura), the gardama (Spodoptera exigua), the caterpillar of the rice cut (Pseudaletia separata), the noctuido dela col (Mamestra brassicae), the malduerme (Agrotis Ipsilon ), the beet worm (Plusia nigrisigna), Thoricoplusia spp., Heliothis spp. and Helicoverpa spp .; white butterflies (Pieridae), such as the white cabbage (Pieris rapae); Leaf winders (Tortricidae), such as Adoxophyes spp., the eastern fruit moth (Grapholita molesta), the soybean pod borer (Leguminivora glycinivorella), the bean pod borer (Matsumuraeses azukivora), the capua (Adoxophyes orana fasciata), the lesser tea tortex (Adoxophyes sp.), the eastern tea tortex (Homona magnanima), the apple leaf winder (Archips fuscocupreanus), the carpocapsa (Cydia pomonella) and the grape sweeper (Lobesia botrana); leaf miners (Gracillariidae), such as the tea leaf miner (Caloptilia theivora) and the apple tree leaf miner (Phyllonorycter ringoneella); Carposinidae, such as the peach moth (Carposina niponensis); leaf mining moths (Lyonetiidae), such as Lyonetia spp .; Herb moths (Lymantriidae), such as Lymantria spp. and Euproctis spp .; Yponomeutidae moths (Yponomeutidae), such as cabbage moth (Plutella xylostella); Gelechid moths (Gelechiidae), such as the peach moth (Anarsia lineatella), the pink cotton worm (Pectinophora gossypiella) and the potato moth (Phthorimaea operculella); tiger moths and allies (Arctiidae), such as the spider webworm (Hyphantria cunea); clothing moths (Tineidae), such as the wrap-forming moth (Tinea translucens) and the clothing weaver moth (Tineola bisselliella); etc.

Thysanoptera:

Trips (Thripidae), such as western flowers thrips (Frankliniella occidentalis), melon thrips (Thrips palmi), yellow tea thrips (Scirtothrips dorsalis), onion thrips (Thrips tabaci), thrips flowers (Frankliniella intonsa), tobacco thrips (Frankliniella fusca), etc.

Diptera:

Housefly (Musca domestica), common mosquito (Culex pipiens pallens), horsefly (Tabanus trigonus), onion fly (Hylemya antiqua), corn seed fly (Hylemya platura) and Anopheles sinensis; leaf-mining flies (Agromyzidae), such as the rice leaf miner (Agromyza oryzae), the small rice leaf miner (Hydrellia griseola), the rice stalk worm (Chlorops oryzae) and the rice miner legumes (Liriomyza trifolii); melon fly (Dacus cucurbitae), fruit fly (Ceratitis capitata); wood moths (Cossidae), such as the yellow wood drill (Zeuzera pyrina), etc.

Coleoptera:

Twenty-eight ladybug (Epilachna vigintioctopunctata), cucurbitaceous leaf beetle (Aulacophora femoralis), striped flea beetle (Phyllotreta striolata), rice leaf beetle (Oulema oryzae), rice weevil (Echninocne gor, squa squara) of rice (Lissorhoptrus oryzophilus), cotton weevil (Anthonomus grandis), southern bean weevil (Callosobruchus chinensis), hunter beetle (Sphenophorus venatus), Japanese beetle (Popillia japonica), cuprous beetle (Anomalous cuprea), worm roots corn (Diabrotica spp.), potato beetle (Leptinotarsa decemlineata), wire worms (Agriotes spp.), tobacco borer (Lasioderma serricorne), carpet weevil (Anthrenus verbasci), chestnut weevil (Tribolium castaneum) ), woodworm (Lyctus brunneus), white spotted longicorn beetle (Anoplophora malasiaca), pine buds beetle (Tomicus piniperda), etc.

Orthoptera:

Migratory lobster (Locusta migratoria), grillotopo (Gryllotalpa africana), rice grasshoppers (Oxya yezoensis), rice grasshoppers (Oxya japonica), etc.

Hymenoptera:

Beet wasp (Athalia rosae), cutting ant (Acromyrmex spp.), Fire ant (Solénopsis spp.), Etc.

Blattodea:

German cockroach (Blattella germanica), "smokybrown" cockroach (Periplaneta fuliginosa), American cockroach (Periplaneta americana), brown cockroach (Periplaneta brunnea), eastern cockroach (Blatta orientalis), etc.

As examples of mites on which the composition of the present invention has a controlling effect, the following accharides are included:

Spider mites (Tetranychidae), such as red spider mite (Tetranychus urticae), red citrus mite (Panonychus citri) and Oligonychus spp .; erythrophic mites (Eriophydae), such as the citrus browning pink mite (Aculops pelekassi); Tarsonémid mites (Tarsonemidae), such as the white mite (Polyphagotarsonemus latus); false spider mites (Tenuipalpidae); Tuckerellidae; acarid mites (Acaridae), such as the storage mite (Tyrophagus putrescentiae); house dust mites (Pyroglyphidae), such as Dermatophagoides farinae and Dermatophagoides ptrenyssnus; and cheiletid mites (Cheyletidae), such as Cheyletus eruditus, Cheyletus malaccensis and Cheyletus moorei.

Preferred examples of pests and mites include aphids (Aphididae), thrips (Thripidae), leaf-mining flies (Agromyzidae), cabbage moth (Plutella xylostella), soybean pod borer (Leguminivora glycinivorella), worm tobacco gray (Spodoptera litura), capua (Adoxophyes orana fasciata), apple leaf winder (Archips fuscocupreanus), carpocapsa (Cydia pomonella), apple tree leaf miner (Phyllonorycter ringoneella), peach moth (Carposina niponensis) ), Oriental fruit moth (Grapholita molesta), leaf-mining moths (Lyonetiidae), such as Lyonetia spp., Herb moths (Lymantriidae), such as Lymantria spp. and Euproctis spp., mealybugs, including mealybugs (Pseudococcidae), psilids (Psyllidae) and weevils.

Pests, mites or both pests and mites can be controlled by applying effective amounts of clothianidine and phosmet to pests, mites or both pests and mites or to places where pests, mites or both live or inhabit. Pests like mites.

Examples of the place where pests, mites or both pests and mites inhabit or inhabit include agricultural land, such as dry land, flooded land, pastures and orchards, and non-farm land.

Pests, mites or both pests and mites can be controlled by applying effective amounts of clothianidine and phosmet to plants or their surroundings or to the soil in which plants grow. Plants, as used here, include plant stems and leaves, plant seeds and plant bulbs. Bulbs, as used herein, include squamous bulbs, corms, rhizomes, tubers, tuberous roots and rhizophores.

For the method of control of pests, mites or both pests and mites of the present invention (hereinafter referred to as "the present control method"), the weight ratio of clothianidine and phosmet used in combination is preferably of 10: 1 to 1: 100, more preferably 1: 2 to 1: 100. However, the weight ratio of clothianidine and fosmet may vary appropriately depending on the species of pests or mites to be controlled.

In the present control method, although clothianidine and phosmet can be applied separately at the same time, clotianidine and fosmet are usually applied in the form of the composition of the present invention for simplicity of application.

The present control method is specifically performed by treating the foliage of the plants, such as application to the foliage; treatment of plant growing areas, such as soil treatment; seed treatment, such as seed disinfection or seed coating; or treatment of plant bulbs, such as seed potatoes.

As specific examples of the treatment of the foliage of the plants, the application to the foliage and the application to the trunk are included, said applications consisting in the application of the present active principles to the surfaces of the plants.

Examples of soil treatment include soil spraying, soil incorporation and soil soaking with a chemical solution (e.g., chemical irrigation, soil injection, chemical dripping).

Examples of a soil treatment object include a planting hole, a planting row, the vicinity of a planting hole, the vicinity of a planting row, the entire surface of a cultivation area, the ground at the foot of a plant, the spaces between plants, the ground around the base of a log, the ground of a main horse, the ground of a horse, a first aid kit, a nursing tray and a nursing bed.

Soil treatment is carried out, for example, before planting, at the time of planting, immediately after planting, in a growing seedling phase, before transplanting, at the time of transplanting

or in a growth phase after transplantation.

For the treatment of the soil, the present active ingredients can be applied simultaneously to the plants, or a solid fertilizer, such as a fertilizer paste, containing the present active ingredients can be applied to the soil.

The present active ingredients can be mixed with a liquid for irrigation. For example, the present active ingredients can be injected into the irrigation equipment (e.g., an irrigation pipe, an irrigation pipe or a sprinkler), mixed with a fluid for irrigation of grooves or mixed with an aqueous culture medium. The present active ingredients can be previously mixed with an irrigation liquid and then the irrigation liquid can be applied by an irrigation method as described above or a suitable irrigation method, such as sprinkling

or the flood

When the present active ingredients are applied to the plants or their surroundings or to the soil in which the plants grow in the present control method, the application amount is usually from 1 to 10,000 g, preferably from 30 to 3,000 g, per 10,000 m2 in terms of the total amount of these active ingredients. However, the amount of application may vary depending on the types of plants to be protected, the species or population size of the pests or mites to be controlled, on the form of formulation, on the frequency of application of atmospheric conditions and the like.

When an emulsifiable concentrate, a hydratable powder or a suspension concentrate is used for the present control method, it is usually applied after dilution with water. In this case, the total concentration of the present active ingredients in the aqueous dilution is usually 0.0001 to 3% by weight, preferably 0.0005 to 1% by weight. When a powder or granule is used for the present control method, it is usually applied as is without dilution.

In the case of seed treatment, the present active ingredients are applied in a total amount normally of 0.001 to 20 g, preferably 0.01 to 5 g, per 1 kg of seeds.

When the present control method is used, plants such as those described below can be protected against damage caused by pests or mites that eat or suck them. Examples of pests or mites are the same as those described above for the composition of the present invention.

The composition or method of the present invention can be used to control pests, mites or both pests and mites in agricultural lands where the plants listed below are grown without causing chemical damage to the plants.

Examples of plants include:

Agricultural crops: corn, rice, wheat, barley, rye, oats, sorghum, cotton, soy, peanut, buckwheat, sugar beet, rapeseed, sunflower, sugarcane, tobacco, etc.

Vegetables: Solanaceae vegetables (eggplant, tomato, green pepper, hot pepper, potato, etc.), cucurbitaceous vegetables (cucumber, squash, zucchini, watermelon, melon, squash, etc.), cruciferous vegetables (Japanese radish, turnip, horseradish , Kohlrabi, Chinese cabbage, cabbage, Chinese mustard, broccoli, coli fl ow, etc.), compound vegetables (burdock, edible chrysanthemum, artichoke, lettuce, etc.), liliaceous vegetables (scallion, onion, garlic, asparagus, etc.) ), umbelliferous vegetables (carrots, parsley, celery, parsnips, etc.), chenopodiaceous vegetables (spinach, chard, etc.), tilled vegetables (Japanese basil, mint, basil, etc.), (strawberry, sweet potato, yam, aroid , etc.).

Flowers and ornamental plants.

Plants with foliage.

Grass.

Fruit trees: grapefruit fruits (apple, common pear, Japanese pear, Japanese quince, quince, etc.), fleshy fruits with bone (peach, plum, nectarine, Japanese plum, cherry, apricot, prune, etc.), citrus plants (satsuma tangerine, orange, lemon, lime, grapefruit, etc.), nuts (chestnut, walnut, hazelnut, almond, pistachio, cashew, macadamia nut, etc.), berry fruits (blueberry, cranberry, blackberry , currant, etc.), grape, persimmon, olive, loquat, banana, coffee, date, coconut, etc.

Trees other than fruit trees: tea, mulberry, trees and shrubs in fl ow, urban trees (ash, birch, dogwood, eucalyptus, ginkgo, lilo, maple, oak, poplar, love tree, Chinese liqueur, banana, zelkova, Yours, fir, Japanese hemlock, spiny juniper, pine, spruce, yew), etc.

The plants described above include plants that have resistance to herbicides, such as HFPD inhibitors (eg, isoxafluoro), ALS inhibitors (eg, imazetapyr, tifensulfuron-methyl), EPSF synthesizing enzyme inhibitors (eg , glyphosate), glutamine synthesizing enzyme inhibitors (eg, glufosinate), acetyl-CoA carboxylase inhibitors (eg, setoxidim), bromoxynil, dicamba and 2,4-D, whose resistance is imparted by a method of classical culture or a genetic engineering technique.

Examples of the herbicide-resistant plant imparted by a classical culture method include rapeseed, wheat, sunflower and rice resistant to ALS imidazolinone inhibitors, such as imazetapyr, which can be purchased commercially under the Product designation Clear fi eldTM (registered trademark); soybeans resistant to ALS sulfonylurea inhibitors, such as tifensulfuron-methyl, which can be purchased commercially under the product name STS soybean; and SR corn resistant to acetyl-CoA carboxylase inhibitors, such as triona oxime herbicides and aryloxyphenoxypropionic acid herbicides. Plants to which resistance to acetyl-CoA carboxylase inhibitors can be found in Proc. Natl Acad. Sci. USA, 1990, vol. 87, pp. 7175-7179.

In addition, mutant acetyl-CoA carboxylase resistant to acetyl-CoA carboxylase inhibitors is described in Weed Science, 2005, vol. 53, pp. 728-746. When a mutant acetyl-CoA carboxylase gene is introduced into a plant by a genetic engineering technique, or when a mutation related to the imparting of resistance into the acetyl-CoA carboxylase gene of a plant is introduced, it can be produced a plant resistant to acetyl-CoA carboxylase inhibitors. In addition, nucleic acids can be introduced for the introduction of a base substitution mutation in plant cells by chemoplasty (see Gura T., 1999, Repairing the Genome's Spelling Mistakes, Science 285: 316-318) to induce a substitution mutation. Site-directed amino acid in the acetyl-CoA carboxylase gene or in the plant ALS gene, and thus a plant resistant to acetyl-CoA carboxylase inhibitors or ALS inhibitors can be produced.

Examples of the herbicide resistance plant imparted by a genetic engineering technique include corn, soybean, cotton, rapeseed and sugar beet cultivars resistant to glyphosate, which can be purchased commercially under the product designations RoundupReadyTM, AgrisureGT, etc.; Glufosinate-resistant corn, soy, cotton and rapeseed cultivars, which can be purchased commercially under the LibertyLinkTM product designations, etc .; and the cultivar of bromoxynil resistant cotton, which can be purchased commercially under the product designation BXN.

The plants described above include plants that have the ability to produce insecticidal toxins, for example selective toxins known to be produced by Bacillus, whose capacity is imparted by a genetic engineering technique.

As examples of the insecticidal toxin, insecticidal proteins derived from Bacillus cereus and Bacillus popilliae are included; δ-endotoxins derived from Bacillus thuringiensis, such as Cry1Ab, Cry1Ac, Cry1F, Cry1Fa2, Cry2Ab, Cry3A, Cry3Bb1 and Cry9C; insecticidal proteins derived from Bacillus thuringiensis, such as VIP 1, VIP 2, VIP 3 and VIP 3A; insecticidal proteins derived from nematodes; toxins produced by animals, such as scorpion toxins, spider toxins, bee toxins and insect-specific nerve toxins; fungal toxins; plant lectin; agglutinin; protease inhibitors, such as trypsin inhibitors, serine protease inhibitors, patatin, cystatin and papain inhibitors; inactivating ribosome proteins (PIR), such as ricin, corn PIR, abrin, luffin, saporin and briodine; steroid metabolising enzymes, such as 3-hydroxysteroid oxidase, ecdysteroid-UDP-glucosyltransferase and cholesterol oxidase; ecdysone inhibitors; HMG-CoA reductase; ion channel inhibitors, such as sodium channel inhibitors and calcium channel inhibitors; juvenile hormone esterase; diuretic hormone receptors; stilbene synthase; bibencil synthase; chitinase; and glucanase.

Other examples of the insecticidal toxin include hybrid toxins, partial deficiency toxins and modified toxins of different insecticidal proteins, for example insecticidal proteins such as δ-endotoxins, such as Cry1Ab, Cry1Ac, Cry1F, Cry1Fa2, Cry2Ab, Cry3A, Cry3Bb1, Cry3Bb1, Cry3Bb1, Cry3Bb1 and Cry35Ab, VIP 1, VIP 2, VIP 3 and VIP 3A. The hybrid toxin is produced by combining different domains of insecticidal proteins through a genetic engineering technique. As an example of the partial deficiency toxin, Cry1Ab is included, in which a part of the amino acids are suppressed. An example of the modified toxin includes a toxin in which one or more of the amino acids of a natural toxin are substituted.

Insecticidal toxins and genetically engineered plants that have the ability to produce insecticidal toxins can be found, for example, in EP-A-0.374.753, WO 93/07278, WO 95/34656, EP-A-0.427 .529, EP-A-451,878 and WO 03/052073.

Genetically engineered plants that have the ability to produce insecticidal toxins are particularly resistant to attack by beetle pests, hemiptera pests, diptera pests, lepidoptera pests and nematodes.

Also known are genetically engineered plants that have one or more pest resistance genes and thus produce one or more insecticidal toxins, and some of them can be purchased commercially. Examples of such genetically engineered plants include YieldGardTM (a corn cultivar that expresses the Cry1Ab toxin), YieldGard RootwormTM (a corn cultivar that expresses the Cry3Bb1 toxin), YieldGard PlusTM (a corn cultivar that expresses the toxins Cry1Ab and Cry3Bb1), Hercules ITM (a corn cultivar that expresses the Cry1Fa2 toxin and phosphoryric N-acetyltransferase (FAT) to impart resistance to glufosinate), NuCOTN33BTM (a cotton cultivar that expresses the Cry1Ac toxin), Bollgard ITM (a cultivar of cotton that expresses the toxin Cry1Ac), Bollgard IITM (a cotton cultivar that expresses the toxins Cry1Ac and Cry2Ab), VIP-COTTM (a cotton cultivar that expresses the toxin VIP), NewleafTM (a potato cultivar that expresses the toxin Cry3A), NatureGard Agrisure GT AdvantageTM (glyphosate resistance character GA21), Agrisure CB AdvantageTM (corn drill character (TM) Bt11) and ProtectaTM.

The plants described above include plants that have the capacity to produce antipathogenic substances, whose capacity is imparted by a genetic engineering technique.

As examples of the antipathogenic substance, PR proteins (PPR described in EP-A-0.392.225) are included; ion channel inhibitors, such as sodium channel inhibitors and calcium channel inhibitors (e.g., toxins KP1, KP4, KP6, etc. produced by viruses); stilbene synthase; bibencil synthase; chitinase; glucanase; and substances produced by microorganisms, such as peptide antibiotics, antibiotics containing heterocycles and protein factors involved in resistance to plant diseases (described in WO 03/000906). Said antipathogenic substances and plants obtained by genetic engineering that produce the antipathogenic substances are described, for example, in EP-A-0.392.225, WO 05/33818 and EP-A-0.353.191.

The plants described above include plants that have beneficial characters, such as a modified oily component or a higher amino acid content, whose characters are imparted by a genetic engineering technique. Examples of plants that have beneficial characteristics include VISTIVETM (low linolenic soybeans that have a reduced content of linolenic acid) and high lysine corn (high oil content) (corn that has a higher lysine content or oil).

Moreover, the plants described above include stacked plants that have a combination of two or more beneficial characters, such as a herbicide-resistant character or a herbicide resistance gene, a pest resistance gene, a gene that produces antipathogenic substances. , a modified oily component and a higher amino acid content, as described above.

Examples

From now on, the present invention will be described in more detail by Formulation Examples and Test Examples, to which the present invention is not limited. In the Examples, the term "part (s)" means part (s) by weight, unless otherwise specified.

Formulation Example 1

Clothianidine (5 parts), fosmet (5 parts), a mixture (35 parts) of white carbon and polyoxyethylene alkyl ether sulfate ammonium (50/50 weight ratio) and water (55 parts) are mixed and divided (55 parts) then finely by a wet grinding method to obtain a suspension concentrate.

Formulation Example 2

Clothianidine (1 part), fosmet (10 parts), a mixture (35 parts) of white carbon and polyoxyethylene alkyl ether sulfate ammonium (50/50 weight ratio) and water (54 parts) are mixed and divided (54 parts) then finely by a wet grinding method to obtain a suspension concentrate.

Formulation Example 3

Clothianidine (20 parts), phosmet (5 parts), sorbitan oleate (1.5 parts) and an aqueous solution (28.5 parts) containing 2 parts of polyvinyl alcohol are mixed and then finely divided by a milling method wet An aqueous solution (45 parts) containing 0.05 parts of xanthan gum and 0.1 part of aluminum and magnesium silicate and then propylene glycol (10 parts) are added to the mixture. The mixture is stirred to obtain a suspension concentrate.

Formulation Example 4

Clothianidine (10 parts), phosmet (2.5 parts), sorbitan oleate (1.5 parts) and an aqueous solution (30 parts) containing 2 parts of polyvinyl alcohol are mixed and then finely divided by a milling method wet An aqueous solution (47.5 parts) containing 0.05 parts of xanthan gum and 0.1 part of aluminum and magnesium silicate and then propylene glycol (10 parts) are added to the mixture. The mixture is stirred to obtain a suspension concentrate.

Formulation Example 5

They are mixed to prepare a clotianidine suspension (5 parts), fosmet (40 parts), propylene glycol (5 parts, manufactured by Nacalai tesque), Soprophor FLK (5 parts, manufactured by Rhodia Nicca), Antifoam C emulsion (0.2 parts, manufactured by Dow Corning Co.), Proxel GXL (0.3 parts, manufactured by Arch Chemicals, Inc.) and water with ion exchange (49.5 parts). Glass beads (150 parts, Φ = 1 mm) are added to the suspension (100 parts), followed by grinding for 2 hours by cooling with cooling water. The glass beads are then removed by filtration to obtain a suspension concentrate.

Formulation Example 6

Clothianidine (1 part), phosmet (4 parts), synthetic hydrated silicon oxide (1 part), calcium ligninsulfonate (2 parts), bentonite (30 parts) and kaolin clay (62 parts) are ground and mixed well. Water is added to the mixture, the mixture is kneaded well, granulated and then dried to obtain a granule.

Formulation Example 7

Clothianidine (1 part), fosmet (2 parts), kaolin clay (85 parts) and talcum powder (10 parts) are ground and mixed to obtain a powder.

Formulation Example 8

Clothianidine (10 parts), phosmet (1 part), calcium ligninsulfonate (3 parts), sodium lauryl sulfate (2 parts) and synthetic hydrated silicon oxide (84 parts) are ground and mixed to obtain a hydratable powder.

Test Example 1

Insecticidal activity test (1) on Spodoptera litura

A solution of clothianidine in acetone and a solution of fosmet in acetone, respectively, were diluted with water and then mixed to prepare a chemical test solution containing clothianidine and phosmet in respective predetermined concentrations. The chemical test solution (2 ml) was injected into an artificial diet (13 g, Insecta LF, manufactured by Nosan Corporation) placed in a polyethylene cup (diameter: 5.5 cm). 5 larvae of fourth stage of Spodoptera litura were then released in the polyethylene cup. At three days, the life or death of the insects was examined.

As a comparison, the same test was performed, except for the fact that it was diluted, either a solution of clothianidine in acetone, or a solution of fosmet in acetone, with water to prepare a solution of clothianidine

or a solution of fosmet in a predetermined concentration and was then used instead of the chemical test solution.

As a control, the same test was performed, except for the use of an artificial diet that was injected with a 1% aqueous solution of acetone.

The control value was calculated according to the following Equation 1.

Control value = 100 x (A-B) / A Equation 1

where:

A: Spodoptera litura survival rate when not treated with chemical agent.

B: Spodoptera litura survival rate when treated with chemical agent.

The previous tests were repeated twice. The results are shown in the following Table 1.

TABLE 1

Test Example 2

Insecticidal activity test (2) on Spodoptera litura

A solution of clothianidine in acetone and a solution of fosmet in acetone with water were respectively diluted and then mixed to prepare a chemical test solution containing clothianidine and fosmet in respective predetermined concentrations. The chemical test solution (2 ml) was injected into an artificial diet (13 g, Insecta LF, manufactured by Nosan Corporation) placed in a polyethylene cup (diameter: 5.5 cm). 5 larvae of fourth stage of Spodoptera litura were then released in the polyethylene cup. After five days, life was examined

or death of insects.

As a comparison, the same test was performed, except for the fact that it was diluted, either a solution of clothianidine in acetone, or a solution of fosmet in acetone, with water to prepare a solution of clothianidine

or a solution of fosmet in a predetermined concentration and was then used instead of the chemical test solution.

As a control, the same test was performed, except for the use of an artificial diet that was injected with a 1% aqueous solution of acetone.

The control value was calculated according to Equation 1. The previous tests were repeated twice. The following Table 2 shows the results.

TABLE 2

As can be seen from Table 1 and Table 2, a mixture of clothianidine and phosmet had a greater insecticidal effect on Spodoptera litura.

Test Example 3

Insecticidal activity test (1) on Adoxophyes orana fasciata

A solution of clothianidine in N, N-dimethylformamide (DMF) and a solution of phosmet in DMF were respectively diluted with water and then mixed to prepare a chemical test solution containing clothianidine and phosmet in respective predetermined concentrations. The chemical test solution (1 ml) was injected into an artificial diet (9 g, Silk Mate 2S, manufactured by Nosan Corporation) placed in a polyethylene cup (diameter: 5.5 cm). 30 larvae of the first stage of Adoxophyes orana fasciata were released in the polyethylene cup. At six days, the life or death of the insects was examined.

As a control, the same test was performed, except for the use of an artificial diet that was injected with a 1% aqueous solution of DMF.

The control value was calculated according to the following Equation 2.

Control value = 100 x (C-D) / C Equation 2

where:

C: Adoxophyes orana fasciata survival rate when not treated with chemical agent.

D: Adoxophyes orana fasciata survival rate when treated with chemical agent. The previous tests were repeated twice. The following Table 3 shows the results.

TABLE 3

As can be seen from Table 3, the composition of the present invention had a greater insecticidal effect on Adoxophyes orana fasciata.

Industrial applicability

According to the present invention, it is possible to have a composition for controlling pests, mites or both pests and mites that has a higher activity, and a method for effectively controlling pests, mites or both pests and mites.

Claims (7)

one.

A composition for controlling pests, mites or both pests and mites, where the composition contains clothianidine and fosmet as active ingredients.

2.

The composition according to claim 1, wherein the weight ratio of clothianidine to phosmet is from 10: 1 to 1: 100.

3.

The composition according to claim 1, wherein the weight ratio of clothianidine to fosmet is 1: 2 to 1: 100.

Four.

A method to control pests, mites or both pests and mites, which consists in applying effective amounts of clothianidine and phosmet to pests, mites or both pests and mites or to places where pests, mites or both pests and mites.

5.

A method to control pests, mites or both pests and mites, which consists in applying effective amounts of clothianidine and phosmet to plants or their surroundings or to the soil in which plants grow.

6. Use of a combination of clothianidine and fosmet to control pests, mites or both pests and mites. SPANISH OFFICE OF THE PATENTS AND BRAND Application no .: 201031358 SPAIN Date of submission of the application: 13.09.2010 Priority Date: REPORT ON THE STATE OF THE TECHNIQUE 51 Int. Cl.: A01N51 / 00 (01.01.2006) A01N57 / 16 (01.01.2006) RELEVANT DOCUMENTS

Category

Documents cited Claims Affected

To

ES 2303646 T3 (BAYER) 16.08.2008 US 2006293287 A1 (JADHAV) 28.12.2006 1-6 1-6

Category of the documents cited X: of particular relevance Y: of particular relevance combined with other / s of the same category A: reflects the state of the art O: refers to unwritten disclosure P: published between the priority date and the date of priority submission of the application E: previous document, but published after the date of submission of the application

This report has been prepared • for all claims • for claims no:

Date of realization of the report 21.02.2011

Examiner M. Ojanguren Fernández Page 1/4

REPORT OF THE STATE OF THE TECHNIQUE Application number: 201031358 Minimum documentation sought (classification system followed by classification symbols) A01N Electronic databases consulted during the search (name of the database and, if possible, terms of search used) INVENES, EPODOC, WPI, CAS State of the Art Report Page 2/4  WRITTEN OPINION Application number: 201031358 Date of Completion of Written Opinion: 02.21.2011 Statement

Novelty (Art. 6.1 LP 11/1986)

Claims Claims 1-6 IF NOT

Inventive activity (Art. 8.1 LP11 / 1986)

Claims Claims 1-6 IF NOT

The application is considered to comply with the industrial application requirement. This requirement was evaluated during the formal and technical examination phase of the application (Article 31.2 Law 11/1986).  Opinion Base.- This opinion has been made on the basis of the patent application as published. State of the Art Report Page 3/4  WRITTEN OPINION Application number: 201031358 1. Documents considered.- The documents belonging to the state of the art taken into consideration for the realization of this opinion are listed below.

Document

Publication or Identification Number publication date

D01

ES 2303646 T3 (BAYER) 08/16/2008

D02

US 2006293287 A1 (JADHAV) 12.28.2006

2. Statement motivated according to articles 29.6 and 29.7 of the Regulations for the execution of Law 11/1986, of March 20, on Patents on novelty and inventive activity; quotes and explanations in support of this statement The object of the present invention is a composition for controlling pests and mites containing clothianidine and fosmet As active ingredients. Document D1 discloses a synergistic mixture of clothianidine and another compound of the chloronicotinyl insecticide series. Furthermore, the document indicates that the mixture may contain more insecticides from different families such as fosmet. Document D2 discloses an insecticidal formulation that has great stability during storage and that contains a chloronicotinyl compound and an organophosphorus compound, in particular imidacloprid and acetate. None of the documents cited, taken alone or in combination, reveal the invention as defined in the claims 1 to 6 of the present application and there are also no suggestions that direct the person skilled in the art towards the invention as set forth in said claims. Therefore, in view of the state of the art, claims 1 to 6 of the present application are considered to be new and have inventive activity. (art 6.1 and 8.1 LP). State of the Art Report Page 4/4