ES2355457A1 - Insect pest or tick controlling composition and method of controlling insect pest or tick - Google Patents

Abstract

The present invention provides a composition for controlling pests, mites or both pests and mites that contains clothianidin and fosmet as active ingredients, a method for controlling pests, mites or both pests and mites that consists of applying effective amounts of clothianidin and fosmet to pests, mites or both pests and mites or to sites where pests, mites, or both pests and mites live or will inhabit, and a method of controlling pests, mites, or both pests and mites that involves applying amounts effective of clothianidin and phosmet to plants or their surroundings or to the soil in which plants grow. The composition of the present invention has increased activity and the method of the present invention can effectively control pests, mites, or both pests and mites. (Machine-translation by Google Translate, not legally binding)

Description

Composition and method to control pests, mites or both.

Field of the Invention

The present invention relates to a composition to control pests, mites or both pests and mites, and with a method of control of pests, mites or pests Like mites

Background of the invention

So far, clotianidine and fosmet as compounds with pesticidal and acaricidal activity (see, for example, The Pesticide Manual - Fourteenth edition, published by BCPC, ISBN 1901396142, p. 209 and p. 828).

Summary of the Invention

The inventors of the present invention they studied in depth and, as a result, they saw that the Clothianidine and fosmet were used in combination to improve the control effect on pests, mites or both pests and Mites. Thus, the present invention was completed.

The present invention provides:

[1] A composition to control pests, mites or both pests and mites, where the composition includes clothianidine and fosmet as active ingredients;

[2] The composition according to [1], where the ratio of Clothianidine to fosmet weights is 10: 1 to 1: 100;

[3] The composition according to [1], where the ratio of Clothianidine to fosmet weights is 1: 2 to 1: 100;

[4] A method to control pests, mites or both pests and mites, which involves applying quantities Effective of clothianidine and fosmet to pests, mites or both pests such as mites, or to places where they inhabit or will inhabit the pests, mites or both pests and mites;

[5] A method to control pests, mites or both pests and mites, which involves applying quantities Effective of clothianidine and fosmet to plants or their plants nearby or to the ground in which the plants are grown.

[6] The use of a combination of clothianidine and fosmet to control pests, mites or both pests and mites.

Detailed description of the preferred embodiments

Clothianidine and fosmet are compounds known and described, for example, in The Pesticide Manual - Fourteenth edition, published by BCPC, ISBN 1901396142, p. 209 and p. 828. Clothianidine and fosmet can be produced by known methods, which have commercial availability.

The weight ratio of clothianidine to fosmet in the composition for the control of pests, mites or pests As of mites of the present invention it is preferably from 10: 1 to 1: 100 (= clothianidine: fosmet), more preferably 1: 2 to 1: 100, although the weight ratio of clothianidine to fosmet may vary properly depending on the pest or mite species that have to be controlled.

The composition of the present invention may be simply a mixture of clothianidine and fosmet. Usually, The composition of the present invention takes the form of a formulation, such as an oily solution, a concentrate emulsifiable, a suspension concentrate, a hydratable powder, a water dispersible granule, a powder, a granule, a microcapsule or a microgranule, which is obtained by mixing clothianidine and fosmet with an inert support and eventually adding a surfactant and other auxiliary agents for formulation to the mixture.

The total amount of clothianidine and fosmet contained in the composition of the present invention is normally 0.1 to 99% by weight, preferably 0.2 to 90% by weight, plus preferably from 1 to 80% by weight, of the weight of the composition. (Hereinafter, reference may be made collectively to the clothianidine and fosmet as "the present principles assets").

The inert support used for the formulation It includes a solid support and a liquid support.

Examples of the solid support include fine powder or granules of minerals, such as kaolin clay, attapulgite clay, bentonite, montmorillonite, acid clay, pyrophyllite, talc, diatomaceous earth and calcite; substances Natural organic, such as corn cobs and flour nutshell; synthetic organic substances, such as urea; salts, such as calcium carbonate and sulfate ammonium; and synthetic inorganic substances, such as the oxide of synthetic hydrated silicon.

As examples of the liquid support, they are included aromatic hydrocarbons, such as xylene, alkyl benzene and methylnaphthalene; alcohols such such as 2-propanol, ethylene glycol, propylene glycol and ethylene glycol monoethyl ether; ketones, such as acetone, cyclohexanone and isophorone; vegetable oils, such as soybeans and cottonseed oil; aliphatic hydrocarbons of Petroleum; esters; dimethyl sulfoxide; acetonitrile; and water.

Examples of the surfactant include anionic surfactants, such as alkyl sulfates, sulphonates alkylaryl, dialkyl sulfosuccinates, ether phosphates polyoxyethylene alkylaryl, lignin sulphonate and sulfonate polycondensates of naphthalene-formaldehyde; nonionic surfactants, such as polyoxyethylene alkylaryl ethers, copolymers of polyoxyethylene alkyl polyoxypropylene blocks and esters of sorbitan and fatty acids; and cationic surfactants, such as alkyltrimethylammonium salts.

As examples of the other auxiliary agents For formulation, water-soluble polymers, such as polyvinyl alcohol and polyvinylpyrrolidone; saccharides, such as gum arabic, alginic acid and a salt thereof, CMC (carboxymethyl cellulose) and xanthan gum; inorganic substances, such as magnesium and aluminum silicate and alumina sol; preservatives; dyes; and stabilizers, such as PAP (phosphate iso-propyl acid) and BHT (2,6-di-tert-butyl-4-methylphenol).

The composition of the present invention may protect plants as described below from damage due to pests or mites that eat or suck.

As examples of pests on which you have controlling effect the composition of the present invention, include:

Hemiptera:

Leafhoppers (Delphacidae), such as the small brown leafhopper ( Laodelphax striatellus ), the brown rice leafhopper ( Nilaparvata lugens ) and the white-backed rice leafhopper ( Sogatella furcifera ), and chicharritas (Deltocephalidae), such as rice chicharrite green ( Nephotettix cincticeps ) and green chicharrite ( Nephotettix virescens ); aphids (Aphididae), such as cotton aphid ( Aphis gossypii ), green peach aphid ( Myzus persicae ), cabbage aphid ( Brevicoryne brassicae ), potato aphid ( Macrosiphum euphorbiae ), Solanaceae aphid ( Aulacorthum solani ), the oat aphid ( Rhopalosiphum padi ) and the black citrus aphid ( Toxoptera citricidus ); Stink bugs (Pentatomidae), such as the green bug ( Nezara antennata ), the bean bug ( Riptortus clavatus ), the rice bug ( Leptocorisa chinensis ), the white spotted spiny bug ( Eysarcoris parvus ), the brown-brown bug ( Halyomorpha mista ) and the bed bug ligus ( Lygus lineolaris ); whiteflies (Aleyrodidae), such as the greenhouse whitefly ( Trialeurodes vaporariorum ), the sweet potato whitefly ( Bemisia tabaci ) and the silver leaf whitefly ( Bemisia argentifolia ); mealybugs (Coccoidea), such as the California red mealybug ( Aonidiella aurantii ), the San José mealybug ( Comstockaspis perniciosa ), the citrus mealybug ( Unaspis citri ), the red mealybug ( Ceroplastes rubens ), the ribbed mealybug ( Icerya purchasi ) and mealybugs (Pseudococcidae); lace bugs (Tingidae); psilids (Psyllidae), such as pear pear ( Cacopsylla pyricola ) and Asian citrus psyllid ( Diaphorina citri ),
etc.

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Lepidoptera:

Pyralids (Pyralidae), such as the rice borer ( Chilo suppressalis ), the yellow rice stem borer ( Tryporyza incertulas ), the rice leaf winder ( Cnaphalocrocis medinalis ), the cotton leaf winder ( Notarcha derogata ), the Indian flour moth ( Plodia interpunctella ), the eastern corn drill ( Ostrinia furnacalis ), the European corn drill ( Ostrinia nubilalis ), the cabbage worm ( Hellula undalis ) and the grass caterpillar meadows ( Pediasia teterrellus ); nocturnal butterflies (Noctuidae), such as the gray tobacco worm ( Spodoptera litura ), the gardama ( Spodoptera exigua ), the caterpillar of the rice cut ( Pseudaletia separata ), the cabbage noctuide ( Mamestra brassicae ), the malduerme ( Agrotis Ipsilon ), the beet worm ( Plusia nigrisigna ), Thoricoplusia spp., Heliothis spp. and Helicoverpa spp .; white butterflies (Pieridae), such as the white cabbage ( Pieris rapae ); Leaf winders (Tortricidae), such as Adoxophyes spp., the eastern fruit moth ( Grapholita molesta ), the soybean pod borer ( Leguminivora glycinivorella ), the bean pod borer ( Matsumuraeses azukivora ), the capua ( Adoxophyes orana fasciata ), the lesser tea tortex ( Adoxophyes sp.), the eastern tea tortex ( Homona magnanima ), the apple leaf winder ( Archips fuscocupreanus ), the carpocapsa ( Cydia pomonella ) and the grape sweeper ( Lobesia botrana ); leaf miners (Gracillariidae), such as the tea leaf miner ( Caloptilia theivora ) and the apple tree leaf miner ( Phyllonorycter ringoneella ); Carposinidae, such as the peach moth ( Carposina niponensis ); leaf mining moths (Lyonetiidae), such as Lyonetia spp .; Herb moths (Lymantriidae), such as Lymantria spp. and Euproctis spp .; Yponomeutidae moths (Yponomeutidae), such as cabbage moth ( Plutella xylostella ); Gelechid moths (Gelechiidae), such as the peach moth ( Anarsia lineatella ), the pink cotton worm ( Pectinophora gossypiella ) and the potato moth ( Phthorimaea operculella ); tiger moths and allies (Arctiidae), such as the spider webworm ( Hyphantria cunea ); clothing moths (Tineidae), such as the wrap-forming moth ( Tinea translucens ) and the clothing weaver moth ( Tineola bisselliella ); etc.

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Thysanoptera:

Trips (Thripidae), such as western flower thrips ( Frankliniella occidentalis ), melon thrips ( Thrips palmi ), yellow tea thrips ( Scirtothrips dorsalis ), onion thrips ( Thrips tabaci ), thrips the flowers ( Frankliniella intonsa ), the tobacco thrips ( Frankliniella fusca ), etc.

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Diptera:

Housefly ( Musca domestica ), common mosquito ( Culex pipiens pallens ), horsefly ( Tabanus trigonus ), onion fly ( Hylemya antiqua ), corn seed fly ( Hylemya platura ) and Anopheles sinensis ; leaf-mining flies ( Agromyzidae ), such as the rice leaf miner ( Agromyza oryzae ), the small rice leaf miner ( Hydrellia griseola ), the rice stalk worm ( Chlorops oryzae ) and the rice miner legumes ( Liriomyza trifolii ); melon fly ( Dacus cucurbitae ), fruit fly ( Ceratitis capitata ); wood moths (Cossidae), such as the yellow wood drill ( Zeuzera pyrina ), etc.

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Coleoptera:

Twenty-eight ladybird ( Epilachna vigintioctopunctata ), cucurbitaceous leaf beetle ( Aulacophora femoralis ), striped flea beetle ( Phyllotreta striolata ), rice leaf beetle ( Oulema oryzae ), rice weevil ( Echninocne gor , squa squara ) of rice ( Lissorhoptrus oryzophilus ), cotton weevil ( Anthonomus grandis ), southern bean weevil ( Callosobruchus chinensis ), hunter beetle ( Sphenophorus venatus ), Japanese beetle ( Popillia japonica ), cuprous beetle ( Anomalous cuprea ), worm roots corn ( Diabrotica spp.), potato beetle ( Leptinotarsa decemlineata ), wire worms ( Agriotes spp.), tobacco borer ( Lasioderma serricorne ), carpet weevil ( Anthrenus verbasci ), chestnut weevil ( Tribolium castaneum) ), woodworm ( Lyctus brunneus ), white spotted longicorn beetle ( Anoplophora malasiaca ), beetle of buds d the pine ( Tomicus piniperda ), etc.

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Orthoptera:

Migratory lobster ( Locusta migratoria ), grillotopo ( Gryllotalpa africana ), rice grasshoppers ( Oxya yezoensis ), rice grasshoppers ( Oxya japonica ), etc.

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Hymenoptera:

Beet wasp ( Athalia rosae ), cutting ant ( Acromyrmex spp.), Fire ant ( Solénopsis spp.), Etc.

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Blattodea:

German cockroach ( Blattella germanica ), "smokybrown" cockroach ( Periplaneta fuliginosa ), American cockroach ( Periplaneta americana ), brown cockroach ( Periplaneta brunnea ), eastern cockroach ( Blatta orientalis ), etc.

As examples of mites on which the Composition of the present invention has a controlling effect, They include the following accharides:

Spider mites (Tetranychidae), such as red spider mite ( Tetranychus urticae ), red citrus mite ( Panonychus citri ) and Oligonychus spp .; Eryophyte mites (Eriophydae), such as the citrus browning pink mite ( Aculops pelekassi ); Tarsonémid mites (Tarsonemidae), such as the white mite ( Polyphagotarsonemus latus ); false spider mites (Tenuipalpidae); Tuckerellidae; acarid mites (Acaridae), such as the storage mite ( Tyrophagus putrescentiae ); house dust mites (Pyroglyphidae), such as Dermatophagoides farinae and Dermatophagoides ptrenyssnus ; and cheiletid mites (Cheyletidae), such as Cheyletus eruditus , Cheyletus malaccensis and Cheyletus moorei .

Preferred examples of pests and mites include aphids (Aphididae), thrips (Thripidae), leaf-mining flies (Agromyzidae), cabbage moth ( Plutella xylostella ), soybean sheath ( Leguminivora glycinivorella ), worm tobacco gray ( Spodoptera litura ), capua ( Adoxophyes orana fasciata ), apple leaf winder ( Archips fuscocupreanus ), carpocapsa ( Cydia pomonella ), apple tree leaf miner ( Phyllonorycter ringoneella ), peach moth ( Carposina niponensis) ), Oriental fruit moth ( Grapholita molesta ), leaf-mining moths (Lyonetiidae), such as Lyonetia spp., Herb moths (Lymantriidae), such as Lymantria spp. and Euproctis spp., mealybugs, including mealybugs (Pseudococcidae), psilids (Psyllidae) and weevils.

You can control pests, mites or both pests and mites applying effective amounts of Clothianidine and fosmet pests, mites or both pests like the mites or the places where they inhabit or will inhabit the pests, mites or both pests and mites.

As examples of where they live or will inhabit pests, mites or both pests and mites, including agricultural land, such as dry land, flooded land, pastures and orchards, and non-agricultural land.

You can control pests, mites or both pests and mites applying effective amounts of Clothianidine and fosmet to plants or their surroundings or to the soil in which plants grow. The plants, as they are used here, include the stems and leaves of the plants, the seeds of plants and plant bulbs. The bulbs, as is used here, include squamous bulbs, corms, rhizomes, tubers, tuberous roots and rhizophores.

For the method of pest control, mites or both pests and mites of the present invention (to which in hereinafter referred to as "the present method of control "), the weight ratio of clothianidine and fosmet used in combination is preferably from 10: 1 to 1: 100, more preferably from 1: 2 to 1: 100. However, the weight ratio of clothianidine and fosmet may vary appropriately depending on the species of pests or mites to control.

In the present control method, although they can apply clotianidine and fosmet separately to it time, clotianidine and fosmet are usually applied as of the composition of the present invention for reasons of simplicity of application

The present control method is specifically  performed by treatment of plant foliage, such as foliage application; treatment of crop areas of plants, such as soil treatment; treatment of seeds, such as seed disinfection or coating of the seeds; or treatment of plant bulbs, such as Sowing potatoes

As specific examples of the treatment of foliage of the plants, the application to the foliage and the application to the trunk, these applications consisting of the application of the present active ingredients to the surfaces of the plants.

As examples of soil treatment, include spraying on the ground, incorporation into the ground and soaking the soil with a chemical solution (e.g. irrigation chemical, soil injection, chemical drip).

As examples of an object of treatment of soil, a planting hole, a row of planting, the vicinity of a planting hole, the in the vicinity of a row of plantation, all of the surface of a crop area, the soil at the foot of a plant, the spaces between plants, the soil around the base of a trunk, the floor of a main horse, the floor of a horse, a first aid kit, a nursing tray and a nursing bed.

The soil treatment is carried out, by example, before planting, at the time of planting, immediately after sowing, in a seedling phase in growth, before transplant, at the time of transplant or in a growth phase after transplantation.

For soil treatment, they can be applied the present active ingredients simultaneously to the plants, or You can apply a solid fertilizer to the soil, such as a paste fertilizer, which contains the present active ingredients.

The present active ingredients can be mixed with a liquid for irrigation. For example, those present active ingredients can be injected into the irrigation equipment (e.g., an irrigation pipe, an irrigation pipe or a sprinkler), mixed with a liquid for irrigation of furrows or mixed with a aqueous culture medium. The present active ingredients can be previously mixed with a liquid for irrigation and then the liquid of irrigation can be applied by means of an irrigation method as it has been described above or a suitable irrigation method, such as the sprinkling or flooding.

When these principles apply active to plants or to their surroundings or to the soil in which plants grow in the present control method, the amount of application is usually 1 to 10,000 g, preferably 30 to 3,000 g, per 10,000 m2 in terms of the total amount of Present active ingredients. However, the amount of application may vary depending on the types of plants that are have to protect, from the species or population size of the pests or mites to be controlled, in the form of formulation, frequency of application, conditions Atmospheric and similar.

When an emulsifiable concentrate is used, a hydratable powder or suspension concentrate for the present Control method, usually applied after dilution with water. In this case, the total concentration of the present principles active in the aqueous dilution is usually 0.0001 to 3% in weight, preferably 0.0005 to 1% by weight. When a powder or granule for the present control method, usually It is applied as is without dilution.

In the case of seed treatment, it apply these active ingredients in a total amount usually 0.001 to 20 g, preferably 0.01 to 5 g, per 1 kg of seeds.

When the present control method is used, it they can protect plants like those described below against Damage caused by pests or mites that eat or suck. The examples of pests or mites are the same as before described for the composition of the present invention.

The composition or method of the present invention can be used to control pests, mites or both pests such as mites on agricultural land where crops are grown plants listed below without causing damage by Chemicals to plants.

Examples of plants include:

Agricultural crops: corn, rice, wheat, barley, rye, oats, sorghum, cotton, soy, peanut, buckwheat, sugar beet, rapeseed, sunflower, sugar cane, tobacco, etc.

Vegetables: Solanaceae vegetables (eggplant, tomato, green pepper, hot pepper, potato, etc.), vegetables cucurbitaceae (cucumber, squash, zucchini, watermelon, melon, zucchini, etc.), cruciferous vegetables (Japanese radish, turnip, Horseradish, Kohlrabi, Chinese cabbage, cabbage, mustard China, broccoli, cauliflower, etc.), compound vegetables (burdock, edible chrysanthemum, artichoke, lettuce, etc.), vegetables Lilies (scallions, onions, garlic, asparagus, etc.), vegetables umbelliferous (carrot, parsley, celery, parsnip, etc.), vegetables chenopodiaceae (spinach, chard, etc.), tilled vegetables (Japanese basil, mint, basil, etc.), (strawberry, sweet potato, yam, aroid, etc.)

Flowers and ornamental plants.

Plants with foliage.

Grass.

Fruit trees: grapefruit fruits (apple, pear common, Japanese pear, Japanese quince, quince, etc.), fruits fleshy with bone (peach, plum, nectarine, Japanese plum, cherry, apricot, prune, etc.), citrus plants (mandarin satsuma, orange, lemon, lime, grapefruit, etc.), fruits dried (chestnut, walnut, hazelnut, almond, pistachio, cashew, walnut of macadamia, etc.), berry fruits (blueberry, cranberry, blackberry, currant, etc.), grape, persimmon, olive, loquat, banana, coffee, date, coconut, etc.

Trees other than fruit trees: tea, mulberry, flowering trees and shrubs, urban trees (ash, birch, dogwood, eucalyptus, ginkgo, lilo, maple, oak, poplar, tree of love, liquidambar of China, banana, zelkova, Japanese you, fir, Japanese hemlock, spiny juniper, pine, spruce, yew), etc.

The plants described above include plants that they have resistance to herbicides, such as inhibitors of HFPD (e.g., isoxaflutol), ALS inhibitors (e.g., imazetapyr, tifensulfuron-methyl), inhibitors of the EPSF synthesizing enzyme (e.g., glyphosate), glutamine synthesizing enzyme inhibitors (e.g., glufosinate), acetyl-CoA inhibitors carboxylase (e.g., setoxidim), bromoxynil, dicamba and 2,4-D, whose resistance is imparted by a method of classical culture or a genetic engineering technique.

As examples of the plant that has resistance  to herbicides imparted by a classical culture method, it include rapeseed, wheat, sunflower and rice resistant to imidazolinone ALS inhibitors, such as imazetapyr, which may be acquired commercially under the product name Clearfield? (Registered trademark); soy resistant to ALS sulfonylurea inhibitors, such as tifensulfuron-methyl, which can be acquired commercially under the product name STS soybean; and the SR corn resistant to inhibitors of acetyl-CoA carboxylase, such as herbicides trione oxime and the herbicides aryloxyphenoxypropionic acid. Be they can find the plants to which resistance is imparted to Acetyl-CoA carboxylase inhibitors in Proc. Natl Acad. Sci. USA, 1990, vol. 87, pp. 7175-7179.

In addition, acetyl-CoA is described mutant carboxylase resistant to inhibitors of acetyl-CoA carboxylase in Weed Science, 2005, vol. 53, pp. 728-746. When a mutant gene is introduced of acetyl-CoA carboxylase in a plant by a genetic engineering technique, or when a mutation related to the imparting of resistance in the gene of The acetyl-CoA carboxylase of a plant can be produce a plant resistant to inhibitors of acetyl-CoA carboxylase. In addition, you can introduce nucleic acids for the introduction of a mutation by base substitution in plant cells for chemotherapy (see Gura T., 1999, Repairing the Genome's Spelling Mistakes, Science 285: 316-318) to induce a mutation by site-directed amino acid substitution in the gene of the acetyl-CoA carboxylase or in the ALS gene of the plant, and thus a plant resistant to acetyl-CoA carboxylase inhibitors or at ALS inhibitors.

As examples of the plant that has resistance  to herbicides imparted by a genetic engineering technique, it include corn, soy, cotton, rapeseed and beet cultivars sugar resistant glyphosate, which can be purchased commercially under product designations RoundupReady?, AgrisureGT, etc .; corn cultivars, soybeans, cotton and rapeseed resistant to glufosinate, which can be commercially acquired under product designations LibertyLink?, Etc .; and the cultivation of cotton resistant to bromoxynil, which can be purchased commercially under the Product designation BXN.

The plants described above include plants that they have the ability to produce insecticidal toxins, for example Selective toxins known to be produced by Bacillus, whose Capacity is imparted by a genetic engineering technique.

As examples of the insecticidal toxin, insecticidal proteins derived from Bacillus cereus and Bacillus popilliae are included ; δ-endotoxins derived from Bacillus thuringiensis , such as Cry1Ab, Cry1Ac, Cry1F, Cry1Fa2, Cry2Ab, Cry3A, Cry3Bb1 and Cry9C; insecticidal proteins derived from Bacillus thuringiensis , such as VIP 1, VIP 2, VIP 3 and VIP 3A; insecticidal proteins derived from nematodes; toxins produced by animals, such as scorpion toxins, spider toxins, bee toxins and insect-specific nerve toxins; fungal toxins; plant lectin; agglutinin; protease inhibitors, such as trypsin inhibitors, serine protease inhibitors, patatin, cystatin and papain inhibitors; inactivating ribosome proteins (PIR), such as ricin, corn PIR, abrina, lufina, saporina and briodina; steroid metabolising enzymes, such as 3-hydroxysteroid oxidase, ecdysteroid-UDP-glucosyltransferase and cholesterol oxidase; ecdysone inhibitors; HMG-CoA reductase; ion channel inhibitors, such as sodium channel inhibitors and calcium channel inhibitors; juvenile hormone esterase; diuretic hormone receptors; stilbene synthase; bibencil synthase; chitinase; and glucanase.

Other examples of insecticidal toxin include  hybrid toxins, partial deficient toxins and toxins modified from different insecticidal proteins, for example insecticidal proteins such as δ-endotoxins, such as Cry1Ab, Cry1Ac, Cry1F, Cry1Fa2, Cry2Ab, Cry3A, Cry3Bb1, Cry9C, Cry34Ab and Cry35Ab, VIP 1, VIP 2, VIP 3 and VIP 3A. The hybrid toxin is produced by combining different domains of insecticidal proteins through a genetic engineering technique As an example of the deficient toxin partial, Cry1Ab is included, in which a part of the amino acids They are suppressed. An example of the modified toxin includes a toxin in which one or more of the amino acids of a natural toxin They are replaced.

You can find the insecticidal toxins and genetically engineered plants that have the ability to produce insecticidal toxins, for example, in EP-A-0.374.753, WO 93/07278, WO 95/34656, EP-A-0.427.529, EP-A-451,878 and WO 03/052073.

Plants obtained by genetic engineering that have the ability to produce insecticidal toxins have particularly resistance to attack by beetle pests, hemiptera pests, diptera pests, lepidoptera pests and nematodes

Plants obtained by genetic engineering that have one or more resistance genes to pests and thus produce one or more insecticidal toxins, and some of They can be purchased commercially. As examples of such plants obtained by genetic engineering, are included YieldGard ™ (a corn cultivar that expresses the Cry1Ab toxin), YieldGard Rootworm? (A corn cultivar that expresses the Cry3Bb1 toxin), YieldGard Plus? (a corn cultivar that expresses toxins Cry1Ab and Cry3Bb1), Hercules I ™ (a grow corn that expresses the toxin Cry1Fa2 and phosphinothricin N-acetyltransferase (FAT) to impart resistance to glufosinate), NuCOTN33B ™ (a cotton cultivar that expresses the Cry1Ac toxin), Bollgard I ™ (a cotton cultivar expressing the Cry1Ac toxin), Bollgard II? (a cultivar of cotton expressing the toxins Cry1Ac and Cry2Ab), VIP-COT ™ (a cotton cultivar that expresses VIP toxin), Newleaf ™ (a potato cultivar that expresses the Cry3A toxin), NatureGard Agrisure GT Advantage? (character of resistance to glyphosate GA21), Agrisure CB Advantage? (corn drill character (TM) Bt11) and Protecta ™.

The plants described above include plants that they have the capacity to produce antipathogenic substances, whose Capacity is imparted by a genetic engineering technique.

As examples of the antipathogen substance, include PR (PPR proteins described in EP-A-0.392.225); inhibitors of ion channels, such as channel inhibitors sodium and calcium channel inhibitors (e.g., toxins KP1, KP4, KP6, etc. produced by viruses); stilbene synthase; bibencil synthase; chitinase; glucanase; and substances produced by microorganisms, such as peptide antibiotics, antibiotics containing heterocycles and protein factors involved in resistance to plant diseases (described in WO 03/000906). These substances are described antipathogens and plants obtained by genetic engineering that produce antipathogenic substances, for example, in EP-A-0.392.225, WO 05/33818 and EP-A-0.353.191.

The plants described above include plants that they have beneficial characters, such as an oily component modified or higher amino acid content, whose characters They are taught by a genetic engineering technique. As examples of plants that have beneficial characters, are included VISTIVE ™ (low linolenic soybeans that have a reduced content of linolenic acid) and high content corn lysine (high oil content) (corn that has a higher lysine or oil content).

Moreover, the plants described above include stacked plants that have a combination of two or more characters beneficial, such as a herbicide resistant character classics or a herbicide resistance gene, a gene of pest resistance, a gene that produces antipathogenic substances, a modified oil component and a higher content in amino acids, as described above.

Examples

From now on, the present will be described invention in more detail by Formulation Examples and Test Examples, to which the present invention is not limited. In the Examples, the term "part (s)" means part (s) by weight, unless specified in contrary.

Formulation Example one

Clothianidine (5 parts), fosmet (5 parts), a mixture (35 parts) of white coal and ammonium salt of polyoxyethylene alkyl ether sulfate (weight ratio 50/50) and water (55 parts) and then finely divided by a method of wet grinding to obtain a suspension concentrate.

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Formulation Example 2

Clothianidine (1 part), fosmet (10 parts), a mixture (35 parts) of white coal and ammonium salt of polyoxyethylene alkyl ether sulfate (weight ratio 50/50) and water (54 parts) and then finely divided by a method of wet grinding to obtain a suspension concentrate.

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Formulation Example 3

Clothianidine (20 parts), fosmet (5 parts), sorbitan oleate (1.5 parts) and an aqueous solution (28.5 parts) containing 2 parts of polyvinyl alcohol and then divide finely by a wet grinding method. Be add to the mixture an aqueous solution (45 parts) containing 0.05 parts of xanthan gum and 0.1 part of aluminum silicate and magnesium and then propylene glycol (10 parts). The mixture is stirred to obtain a suspension concentrate.

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Formulation Example 4

Clothianidine (10 parts), fosmet (2.5 parts), sorbitan oleate (1.5 parts) and an aqueous solution (30 parts) which contains 2 parts of polyvinyl alcohol and divide then finely by a wet grinding method. Are added to the mixture an aqueous solution (47.5 parts) containing 0.05 xanthan gum parts and 0.1 part aluminum silicate and magnesium and then propylene glycol (10 parts). The mixture is stirred to Get a suspension concentrate.

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Formulation Example 5

They are mixed to prepare a suspension clothianidine (5 parts), fosmet (40 parts), propylene glycol (5 parts, manufactured by Nacalai tesque), Soprophor FLK (5 parts, manufactured by Rhodia Nicca), Antifoam C emulsion (0.2 parts, manufactured by Dow Corning Co.), Proxel GXL (0.3 parts, manufactured by Arch Chemicals, Inc.) and water with ion exchange (49.5 parts). Glass beads (100 parts) are added to the suspension (150 parts, Ph = 1 mm), followed by grinding for 2 hours cooling with cooling water. The pearls are then removed glass by filtration to obtain a concentrate in suspension.

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Formulation Example 6

They are ground and clotianidine mixed well (1 part), fosmet (4 parts), synthetic hydrated silicon oxide (1 part), calcium ligninsulfonate (2 parts), bentonite (30 parts) and kaolin clay (62 parts). Water is added to the mixture, it is Knead the mixture well, granulate and then dry to obtain a granule.

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Formulation Example 7

They grind and mix clotianidine well (1 part),  fosmet (2 parts), kaolin clay (85 parts) and talc (10 parts) to get a powder.

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Formulation Example 8

They grind and mix clotianidine well (10 parts), fosmet (1 part), calcium ligninsulfonate (3 parts), sodium lauryl sulfate (2 parts) and hydrated silicon oxide synthetic (84 parts) to obtain a hydratable powder.

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Test Example one

Insecticidal activity test (1) on Spodoptera litura

A solution of clothianidine in acetone and a solution of fosmet in acetone, respectively, were diluted with water and then mixed to prepare a chemical test solution containing clothianidine and phosmet in respective predetermined concentrations. The chemical test solution (2 ml) was infiltrated in an artificial diet (13 g, Insecta LF, manufactured by Nosan Corporation) placed in a polyethylene cup (diameter: 5.5 cm). 5 larvae of fourth stage of Spodoptera litura were then released in the polyethylene cup. At three days, the life or death of the insects was examined.

As a comparison, the same test was performed, except for the fact that it was diluted, or a solution of clothianidine in acetone, or a solution of fosmet in acetone, with water to prepare a clotianidine solution or solution of fosmet at a predetermined concentration and was then used in Place of chemical test solution.

As a control, the same test was performed, except for the use of an artificial diet that infiltrated with a 1% aqueous solution of acetone.

The control value was calculated according to next Equation 1.

Equation 1 Value control = 100 x (A-B) / A

where:

A: Spodoptera litura survival rate when not treated with chemical agent.

B: Spodoptera litura survival rate when treated with chemical agent.

The previous tests were repeated twice. The results are shown in the following Table 1.

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TABLE 1

one

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Test Example 2

Insecticidal activity test (2) on Spodoptera litura

A solution of clothianidine in acetone and a solution of fosmet in acetone with water were respectively diluted and then mixed to prepare a chemical test solution containing clothianidine and fosmet in respective predetermined concentrations. The chemical test solution (2 ml) was infiltrated in an artificial diet (13 g, Insecta LF, manufactured by Nosan Corporation) placed in a polyethylene cup (diameter: 5.5 cm). 5 larvae of fourth stage of Spodoptera litura were then released in the polyethylene cup. At five days, the life or death of the insects was examined.

As a comparison, the same test was performed, except for the fact that it was diluted, or a solution of clothianidine in acetone, or a solution of fosmet in acetone, with water to prepare a clotianidine solution or solution of fosmet at a predetermined concentration and was then used in Place of chemical test solution.

As a control, the same test was performed, except for the use of an artificial diet that infiltrated with a 1% aqueous solution of acetone.

The control value was calculated according to the Equation one.

The previous tests were repeated twice. The following Table 2 shows the results.

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TABLE 2

2

As can be seen from Table 1 and Table 2, a mixture of clothianidine and phosmet had a greater insecticidal effect on Spodoptera litura .

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Test Example 3

Insecticidal activity test (1) on Adoxophyes orana fasciata

A solution of clothianidine in N, N-dimethylformamide (DMF) and a solution of phosmet in DMF were respectively diluted with water and then mixed to prepare a chemical test solution containing clothianidine and phosmet in respective predetermined concentrations. The chemical test solution (1 ml) was infiltrated in an artificial diet (9 g, Silk Mate 2S, manufactured by Nosan Corporation) placed in a polyethylene cup (diameter: 5.5 cm). 30 larvae of the first stage of Adoxophyes orana fasciata were released in the polyethylene cup. At six days, the life or death of the insects was examined.

As a control, the same test was performed, except for the use of an artificial diet that infiltrated with a 1% aqueous solution of DMF.

The control value was calculated according to next Equation 2.

Equation 2 Value control = 100 x (C-D) / C

where:

C: Adoxophyes orana fasciata survival rate when not treated with chemical agent.

D: Adoxophyes orana fasciata survival rate when treated with chemical agent.

The previous tests were repeated twice. The following Table 3 shows the results.

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TABLE 3

3

4

As can be seen from Table 3, the composition of the present invention had a greater insecticidal effect on Adoxophyes orana fasciata .

Industrial applicability

According to the present invention, it is possible to arrange  of a composition to control pests, mites or pests as mites that have a higher activity, and a method for effectively control pests, mites or both Pests like mites.

Claims (6)

1. A composition to control pests, mites or both pests and mites, where the composition contains Clothianidine and fosmet as active ingredients. 2. The composition according to claim 1, where the weight ratio of clothianidine to fosmet is 10: 1 a 1: 100 3. The composition according to claim 1, where the weight ratio of clothianidine to fosmet is 1: 2 a 1: 100 4. A method to control pests, mites or both pests and mites, which involves applying quantities Effective of clothianidine and fosmet to pests, mites or both pests such as mites or places where they live or will inhabit pests, mites or both pests and Mites. 5. A method to control pests, mites or both pests and mites, which involves applying quantities Effective of clothianidine and fosmet to plants or their plants nearby or to the ground where plants grow. 6. Use of a combination of clothianidine and fosmet to control pests, mites or both pests and mites.