KR20200114215A - Method for improving growth and development of strawberry runner and runner plant using gibberellin - Google Patents

Abstract

The present invention relates to a method for improving the growth and development of strawberry runners and seedlings by treating 80 to 120 mg·L^-1 gibberellin with medium drainage during seedling of ′Maehyang′ strawberries. The method of the present invention can contribute to improving the productivity of strawberry seedlings.

Description

Method for improving growth and development of strawberry runner and runner plant using gibberellin}

The present invention relates to a strawberry runner and a method for improving growth of seedlings using gibberellin.

Strawberries are herbaceous perennial plants belonging to the Rosaceae family, with a domestic cultivation area of 5,907 ha in 2017 and production of 198,000 tons, one of the representative fruits and vegetables recording the world's 9th largest production (Korea Agro-Fisheries & Food Trade Corporation). (aT). 2017. Import and export statistics. http://www.at.or.kr; Korea Rural Economic Institute (KREI). 2017. Import and export statistics. http://www.krei.re.kr) . Strawberries are classified into field cultivation and facility cultivation methods, of which facility cultivation accounts for 98%, and by region, Gyeongnam 36.5%, Chungnam 20.9%, Jeonbuk 12.8%, and Jeonnam 14.8% (Korean Statistical Information Service (KOSIS) ).2016.Import and export statistics.http://kosis.kr). Korea's strawberry exports are 5,106 tons and exports are 43,978 USD, the second largest export of fresh fruits and vegetables after paprika, but the export ratio is very low at 2.4% compared to total production. 'Maehyang' strawberries, a major export variety, account for 3.3% of strawberry varieties grown in Korea, and are mainly grown in Jinju, Gyeongnam (Korea Agricultural Trade Information (KATI). 2017. 2017 strawberry domestic production and foreign market trends. https://www.kati.net).

'Maehyang', an export variety, was bred at the Nonsan Strawberry Research Center in 2001, and has a high superfine, high sugar content, low acidity, and excellent hardness for good storage. In addition, due to its low low temperature requirement, dormancy is shallow, flower bud differentiation is fast, and long-term fruit harvesting is possible, making it suitable for forcible cultivation (Rural Development Administration (RDA). 2018. Strawberry. Human culture arirang, Seoul, Korea, pp. 32 -37). The domestic strawberry cultivation pattern is shifting from semi-contact cultivation to cultivation for long-term high yield. Chokseong cultivation is a cultivation method in which strawberry seedlings are planted in mid- and late March, seedlings generated from the seedlings are grown, flower buds are differentiated, and flower buds are formed in early and mid September to produce fruit. . In this series of processes, the seedling quality and initial growth of the mother seed determine the number of runners, the production amount of the seedling, the seedling quality of the seedling, and the growth and fruit production of the seedling after planting.

In strawberry cultivation, seedling quality of seedlings is a decisive factor affecting the growth and yield after planting. Therefore, in response to the recent demands of strawberry cultivation farmers, there is a growing interest in producing good seedlings. In line with this trend, in Korea, various types of cultivation according to the variety, the timing of cutting the seedling device runners and the timing of stopping the supply of nutrients, finding out the electrical conductivity (EC) concentration of nutrient solution, the red leaves of the seedlings, and finding out the amount of fertilizer supply in the seedling period. Research is ongoing. However, studies on the treatment of growth regulators to improve the productivity of the runner and seedling of the seedling strawberry and the growth of the parent stem are still insufficient.

The present invention was carried out to find out how to treat gibberellin and an appropriate concentration for the mother strain of'Maehyang' strawberries, and to confirm the possibility of field application through the initial growth of the mother strain, production of runners, and improvement of the seedling quality of the seedling.

On the other hand, Korean Patent Publication No. 2018-0050896 discloses a'growth control agent treatment method for production of a runner during seedling of strawberry maehyang using synthetic cytokinins, benzylaminopurine', and Korean Patent No. 1886600 discloses sodium hydrogen sulfide. A'strawberry runner root growth method' using the mixed liquid is disclosed, but there is no description of a strawberry runner and a method of improving the growth of seedlings using gibberellin of the present invention.

The present invention was derived from the above requirements, and the present inventors treated 50, 100 or 200 mg·L ^-1 of gibberellin once by foliar spraying or drenching the medium during the seedling of'Maehyang' strawberries. As a result, the number of runners and seedlings was largely produced in the high concentration of giverelin treatment regardless of the treatment method, but it was confirmed that the crown grows abnormally long in the experimental group treated with high concentration of giverellin. In addition, the seedling quality of the seedlings was confirmed to be superior to the foliar spraying in the medium drench as the concentration of gibberellin was lowered.In order to improve the growth of the runner and seedlings of the seedling'Maehyang' strawberry, 100mg·L ^-1 of giverelin was treated with the medium drench By confirming that it is suitable to do, the present invention has been completed.

In order to solve the above problems, the present invention provides a method for improving the growth of a runner and a seedling of a strawberry, comprising the step of seeding a strawberry seedling by treating a gibberellin.

In addition, the present invention provides a composition for improving the growth of plant strawberry runners and seedlings, containing 80 to 120mg·L ^-1 gibberellin as an active ingredient.

In the present invention, by finding out the application method and concentration of gibberellin during seedling of'Maehyang' strawberries, it will not only improve the growth of seedlings and runners, but also increase the incidence of seedlings, thereby contributing to the improvement of productivity of strawberry seedlings.

1 is a result of analysis of the number of runners (A) and the number of seedlings (B) of'Maehyang' strawberries at 10 weeks after gibberellin treatment. control; No giverelin treatment, Spray; Foliar spray, Drench; Badge drench.
2 is a result of analyzing the ratio of physiological disorders occurring in the tube part of the'Maehyang' strawberry parental measured 100 days after the giverelin treatment.

In order to achieve the object of the present invention, the present invention provides a method for improving the growth of a runner and a seedling of a strawberry, comprising the step of seeding a strawberry seedling by treating a gibberellin.

Gibberellin is a hormone-based growth regulator used to regulate the growth of plants, and is known to promote growth, determine flowering induction and sex, and has been reported to promote seed development, pollen development and pollen tube growth.

In the method of the present invention, the treatment of gibberellin may be treated with a drench method at a concentration of 80 to 120 mg·L ^-1 to the strawberry seedlings 28 to 32 days after planting, more preferably After the 30th day, the strawberry mother stock may be treated with a one-time medium irrigation method at a concentration of 100mg·L ^-1 , but is not limited thereto.

In the method according to an embodiment of the present invention, the strawberry may be a'Maehyang' variety. The response of strawberry varieties to gibberelin has been reported to show differences between varieties due to differences in endogenous hormone levels (Lopez-Galarza et al. (1989) International Strawberry Symposium 265'The influence of winter gibberellic acid applications on earliness. , productivity and other parameters of quality in strawberry cultivation ( Fragaria × ananassa Duch.) on the spanish mediterranean coast.'), the specific concentration of giverelin and treatment method may differ depending on the variety.

The present invention also provides a composition for improving the growth of plant strawberry runners and seedlings containing 80-120mg·L ^-1 of gibberellin as an active ingredient. The composition of the present invention contains as an active ingredient gibberellin adjusted to 80 to 120 mg L ^-1 , which is an optimal concentration condition that can improve not only the growth of the runner, but also the growth of the generated seedlings during the seedling of strawberries in facilities, When the composition according to the present invention is used for planting strawberries, productivity of runners and seedlings of strawberries can be improved. The facility strawberry may be a'Maehyang' variety.

Hereinafter, the present invention will be described in detail by examples. However, the following examples are only illustrative of the present invention, and the contents of the present invention are not limited to the following examples.

Materials and methods

1. Experimental materials and cultivation environment

Only the homogeneous'Maehyang' strawberry ( Fragaria × ananssa Duch. cv. Maehyang) seedlings with fully developed three leaves were selected, and on March 20, 2018, the strawberry-only mixed top soil (BC2, BVB substrates Co. Ltd., De Lier, The Netherlands) in a dedicated strawberry cultivation pot (64×27×18cm, Hwaseong Industrial Co. Ltd., Hwaseong, Korea), and cultivated in a glass greenhouse at the affiliated farm of Gyeongsang National University until June 27. During the growing season, was drenched with a drip tape, Netherlands Bas Van Buuren (BVB) Strawberry-only medium (massive elements in the composition is _{^{NO 3 - 8.2, NH 4 +}} 1.1, H 2 PO 4 - 1.7, K + 4.9, Ca ²⁺ 5.2, Mg ²⁺ 2.2, SO ₄ ^2- 3.5me·L ^-1 , trace elements are Fe-EDTA 10.60, H ₃ BO ₃ 0.31, CuSO ₄ 5H ₂ O 0.16, MnSO ₄ 5H ₂ O 2.54, Na ₂ MoO ₄ ·2H ₂ O 0.12, ZnSO ₄ ·7H ₂ O 2.21mg ·L ^-1 ) was used after preparation by reflecting the result of raw water analysis. After planting, the culture medium was sufficiently irrigated so that the medium did not dry out for root establishment by setting the EC level to 1.5 dS·m ⁻¹ . After root establishment, the amount of culture medium was supplied at 200-450 mL per strawberry cultivation pot, 1-2 times a day (15 minutes per time) on a clear day, and not supplied on a cloudy day. During the cultivation period, the seedlings and old leaves were periodically removed for the management of the seedlings, and the runners from the seedlings were not removed to confirm the effect of the growth regulator. In addition, cyflufenamid (3.5%), abamectin (1.8%), imidacloprid (10%) and acetamiprid (5%), and azoxystrobin, respectively, to control powdery, mite, aphids, anthrax, and small root flies, which are the major pests that occur during strawberry cultivation. (21.7%) was sprayed every 5-7 days.

2. Growth regulator treatment

The growth regulator used in the present invention was gibberellin (Gibberellin A ₃ , GA ₃ ) (Dea-yu gibberellin, Dea yu Co. Ltd., Gyeongsan, Korea), and Lee et al. (2013) on the 30th day after planting. With reference to the concentration of GA ₃ in a runner and seedling production study of strawberry seedlings using gibberellin by Ku et al. (1998), the concentration of 0, 1, 2, 4 times gibberellin based on GA ₃ 50mg·L ^-1 It was prepared with 0, 50, 100, 200mg·L ^-1 , and 45mL per plant was applied with foliar spray and medium irrigation, respectively.

3. Survey items

In order to compare the growth of'Maehyang' strawberries according to the growth regulator treatment method and concentration, plant height, leaf length, leaf width, crown diameter, number of runners, runner length, runner diameter, soil plant analysis development ( SPAD) was measured. Plant length was measured based on the largest leaf, and leaf length and leaf width were measured based on the first new leaf after treatment. The crown diameter was measured at 1 cm at the top of the paper part using a vernier caliper (CD-20CPX, Mitutoyo Co. Ltd., Kawasaki, Japan). The number of runners was measured by the number of runners generated after treatment, the runner length was measured based on the first runner, and the runner diameter was measured at 1 cm from the top of the first runner on the mother column side. The SPAD value was measured using a chlorophyll meter (SPAD-502, Konica Minolta Inc., Tokyo, Japan) to measure the fully developed leaves, and 12 individuals were randomly selected from the treatment zones for each concentration to determine whether physiological disorders at the crown of the parent line were Therefore, it was considered that physiological disorders occurred when the crown was painted over 5cm. Ten weeks after treatment, the growth characteristics of runners and seedlings were investigated according to the treatment method and concentration of the growth regulator. The number of runners, the length of the runners, the live weight and dry weight of the runners were investigated. The growth characteristics of the seedlings were the number of seedlings, the length of the first, second, and third seedlings, leaf length, leaf width, leaf area, and crown. Diameter, fresh weight and dry weight, and SPAD were investigated. The leaf area was measured using a leaf area measuring instrument (LI-3000, LICOR Inc., Nebraska-Lincoln USA). Live weight and dry weight were measured using an electronic balance (EW220-3NM, Kern&Sohn GmbH., Balingen, Germany), and dry weight was dried for 72 hours in a constant temperature dryer (Venticell-222, MMM Medcenter Einrichtungen GmbH., Planegg, Germany). It was measured using a sample. The EC level and pH of the nutrient solution were measured using a portable pH/EC measuring system (Enzo 8200m, GOnDO Electronic Co. Ltd., Taipeo, Taiwan).

4. Statistical analysis

The batch of the experiment was arranged by the egg mass method of 3 repetitions of 4 specimens per treatment to determine the treatment method and concentration of GA3 including the untreated group. Statistical analysis was performed by ANOVA using a SAS program (SAS 9.4, SAS Institute Inc., Cary, USA), and the comparison between the means was 5% significant using Duncan's multiple range test. At the level, the significance between each treatment was verified. The graph was shown using the SigmaPlot program (Sigma Plot 12.0, Systat Software Inc., San Jose, USA).

Example 1. Analysis of parental growth characteristics of strawberry'Maehyang' according to gibberellin treatment method and concentration

Table 1 shows the results of examining the growth of strawberry'Maeyang' measured at 7 weeks after GA ₃ treatment by a non-destructive method.

Plant length, leaf length, and leaf width were significantly longer in the 200mg·L ^-1 foliar-sprayed treatment group, and the runner length was also the longest at 123.8cm in the 200mg·L ^-1 foliar-sprayed treatment. The runner diameter was thick in the 50mg·L ^-1 and 200mg·L ^-1 foliar sprayed treatments, but there was no positive correlation according to the concentration. However, the SPAD value was the lowest in the foliar sprayed treatment at 200mg·L ^-1 , and the size of strawberry leaf cells increased by giverelin, resulting in an increase in leaf area, and it was judged that the SPAD value decreased due to a decrease in the chlorophyll concentration per unit area. Crown diameter and number of runners did not show any difference depending on the treatment method and concentration of GA ₃ . When growing strawberries, foliar spraying with a growth regulator improved the growth of strawberries in terms of growth characteristics such as number of leaves, crown diameter, and leaf area compared to the control. In the present invention, when comparing the non-treatment and GA ₃ treatment, the treatment of GA ₃ showed the effect of enhancing the growth of strawberries. Most of the photosynthetic products of strawberries are used for vegetative growth during the seedling period of the high temperature long day, and it was judged that the treatment of GA ₃ during this period further promoted the growth. In the GA ₃ treatment method, foliar spraying showed the effect of increasing growth compared to medium drench. When treated with growth regulators, foliar spraying has the advantage of absorbing the drug through the leaves so that the effect can be achieved quickly, whereas a large amount of growth regulators are required, and the medium drenching is slow, but it can produce many effects at low concentrations for a long time. have. Therefore, growth was further enhanced in the initial foliar spraying treatment affected by GA ₃ . Also increased GA ₃ treatment of strawberry The higher the concentration, regardless of the treatment was prolonged such as plant height, leaf length, leaf width, the size of the strawberry plants at the greatest treatment of the concentration of GA ₃ highest 200mg · L ^-1 .

Example 2. Analysis of growth characteristics of runners and seedlings of strawberry'Maehyang' according to gibberellin treatment method and concentration

The result of measuring the number of runners and seedlings of'Maehyang' strawberry at 10 weeks after GA ₃ treatment was produced as the concentration of the growth regulator increased, regardless of the treatment method (FIG. 1). According to the above results, the GA ₃ treatment of'Maehyang' strawberry promoted the growth of the parental column with higher concentration regardless of the treatment method. However, as the concentration of GA ₃ treatment increased, the crown of the parental column was abnormally lengthened (FIG. 2). Foliar spraying caused more crown deformities than medium drenching, which is believed to be due to the fact that foliar spraying rapidly absorbed the growth regulator and affected more. The present invention was carried out from the viewpoint of growth of parental stems through GA ₃ treatment and production of runners and fine seedlings, but deformities occurred in the crown, and further studies on the investigation of appropriate treatment concentrations that can promote the growth of parental stems while lowering crown anomalies in the future It is judged to be necessary.

As a result of measuring the seedling quality of the first, second, and third seedlings that were not separated from the strawberry seedlings 10 weeks after GA ₃ treatment, the plant height of the first seedling was the highest in the 50mg·L ^-1 medium drenched treatment. , Leaf width was significantly higher in the control group in the treatment with 100mg·L ^-1 infusion, and leaf area, crown diameter, fresh weight and dry weight were significantly higher in the control group (Table 2).

The second seedling had the largest plant height and leaf area in the medium-drenched treatment of 100mg·L ^{-1 and} the highest SPAD value (Table 3). Leaf length, leaf width, fresh weight and dry weight were the highest in the control.

The third seedling length was highest in the treatment with 100mg·L ^-1 medium, but the remaining growth characteristics were significantly higher in the control group than in the treatment group (Table 4). In the treatment method, it was confirmed that the growth of seedlings in medium irrigation and low concentration treatment was better.

In previous studies, there has been a report that giverelin promotes rooting of the main root and suppresses the formation of adventitious roots.In the present invention, the growth of the basement of the treated area was sluggish due to the treatment of giverellin of the parent, and more assimilation to the vegetative growth of the above-ground part in the initial growth. By using the product, it was judged that the growth of the control was relatively better. In the present invention, the number of runners and seedlings of'Maehyang' strawberry was most often produced in the foliar spraying treatment of 200mg·L ^-1 , but deformity of the crown occurred at high concentration of GA ₃ , and negative effects were observed on seedling quality. In strawberry cultivation, seedlings are important for the growth of mothers and the number of runners and seedlings, but they must have thick crowns, long grasses, and strong seedlings. This can be confirmed through reports that high-quality seedlings with a crown diameter of 10 mm or more increased the number of flowers and yields due to the increased accumulation of inorganic nutrients required for growth and development after planting.The larger the crown size of strawberry seedlings, the more root establishment and growth. The crown diameter, which is excellent and has a study result that flowering is fast, plays an important role in strawberry cultivation.

Taking the above results together, in consideration of the growth of'Maehyang' strawberry seedlings and seedling quality such as runner and seedling production, it is judged that the most suitable treatment method is to inject the medium with GA ₃ at 100mg·L ^-1 .

Claims (5)

A method for improving the growth of a runner and a seedling of a strawberry, comprising the step of seeding a strawberry seedling with a gibberellin. The method according to claim 1, wherein 80 to 120 mg·L ⁻¹ of gibberellin is treated. The method of claim 1, wherein the giverelin is treated with a drench on the strawberry seedlings 28 to 32 days after planting. The method of claim 1, wherein the strawberry is a'Maehyang' variety. 80 ~ 120mg · L ^-1 containing gibberellin (gibberellin) as an active ingredient, a composition for improving the growth of plant strawberry runners and seedlings.

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