KR102359498B1 - Method for suppressing lateral bud development of mother plant and improving growth of daughter plant in strawberry using indole-3-butyric acid - Google Patents

Abstract

The present invention relates to a method for inhibiting lateral embryogenesis and improving seedling growth of strawberry mother lines by treating 30-120 mg·L ^-1 of indole-3-butyric acid (IBA) with medium irrigation during facility strawberry seedling. This method may contribute to the improvement of the productivity of strawberry seedlings.

Description

Method for suppressing lateral bud development of mother plant and improving growth of daughter plant in strawberry using indole-3-butyric acid using IBA

The present invention relates to a method for inhibiting lateral bud formation and promoting seedling growth using indole-3-butyric acid (IBA).

Strawberry is an herbaceous perennial plant belonging to the Rosaceae family. In 2017, the domestic cultivated area was 5,907ha and the production was 198,000 tons, one of the representative fruits and vegetables that record the world's ninth largest production. The main export variety, 'Maehyang' strawberries, accounts for 3.3% of the strawberry varieties grown in Korea, and is mainly grown in Jinju, Gyeongnam (Korea Agricultural Trade Information (KATI). 2017. 2017 strawberry domestic production and foreign market trends. https://www.kati.net).

In strawberry cultivation, the seedling quality of the seedlings is a decisive factor influencing the growth and yield after planting. Therefore, according to the demands of strawberry growers, interest in producing excellent seedlings is increasing. In line with this trend, in Korea, research on seedlings such as various cultivation patterns according to varieties, identification of the timing of nutrient supply interruption during the breeding season, electrical conductivity (EC) concentration of nutrient solution, red leaf status of young seedlings, and the amount of fully effective fertilizer supply in the breeding period are being conducted in Korea. have. However, there are still insufficient studies on the treatment of growth regulators for inhibiting the generation of lateral buds, improving the production of runners, and improving the productivity of the seedlings during the seedling stage.

The crown of strawberries is a place where assimilation products are stored, and for seasonal strawberries, including 'Maeyang', to prevent the necessary nutrients from being dispersed during the growth of the crown, lateral buds generated from the crown during the seedling period are removed, so that the tube diameter of 8mm or more is excellent. to produce graves. However, in the process of removing lateral buds, strawberry seedlings are subjected to partial stress in the above and underground parts, and if the accumulated stress is severe, the seedlings may die. Therefore, if it is possible to reduce the occurrence of lateral buds in facility strawberries, it is possible to increase the productivity of strawberry seedlings, and it is possible to reduce the labor required for lateral bud removal.

On the other hand, Korea Patent Application Publication No. 2018-0050896 discloses a 'growth regulator treatment method for runner production during seedling of facility strawberry plum blossoms' using benzylaminopurine, a synthetic cytokinin, and Korean Patent No. 1886600 discloses sodium hydrogen sulfide. Although a 'strawberry runner root growth method' using the mixed solution is disclosed, there is no description of a method for inhibiting lateral bud formation and promoting seedling growth using the IBA of the present invention.

The present invention was derived from the above needs, and the present inventors have provided an effective method for treating indole-3-butyric acid (IBA) and In order to find out the concentration, the number of lateral buds (lateral eyes) of the mother line and the growth characteristics of the mother line and the seedlings were investigated by treatment with IBA at a concentration of 50, 100, 150 or 200 mg L ^-1 by foliar spraying or medium irrigation. , it was confirmed that the lateral embryogenesis of the mother line was decreased in the IBA-treated group compared to the untreated group, but it was found that there was no significant difference between the IBA concentration and the treatment method in the level of suppression of the embryonic generation and the growth characteristics of the mother line, primary and secondary seedlings. However, the growth of tertiary seedlings (leaf length, leaf length, leaf width, tube diameter, live weight and dry weight) was improved in the group treated with medium irrigation of 100 mg L ^-1 IBA, and the number of seedlings in the group treated with IBA foliar application was improved. It was observed that a decrease compared to the untreated group was observed, and when considering the suppression of the generation of lateral buds and the growth of the tertiary seedlings, the present invention was completed by confirming that it was appropriate to treat 100 mg·L ^-1 of IBA by the medium irrigation method. .

In order to solve the above problems, the present invention provides a method for inhibiting lateral embryogenesis and promoting the growth of seedlings, comprising the step of seedling seedlings by treating indole-3-butyric acid (IBA) in strawberry mother lines.

In addition, the present invention provides a composition for inhibiting the generation of lateral buds and promoting the growth of seedlings during facility cultivation, containing 30-120 mg·L ^-1 of IBA as an active ingredient.

The present invention suppresses the generation of lateral buds in the mother line and improves the number of runners as well as the growth of seedlings by finding the application method and concentration of indole-3-butyric acid (IBA), a growth regulator during seedling of 'Maehyang' strawberries. It will be possible to contribute to the improvement of productivity of strawberry 'maehyang' seedlings during facility cultivation by raising the

1 is a layout diagram of an experiment for suppressing lateral bud formation and promoting seedling growth of strawberry 'Maehyang' according to the IBA treatment method and concentration. drench: medium drench, foliar spray: foliar spray.
2 is a photograph showing the appearance of IBA treatment for suppressing the generation of lateral buds of strawberry 'Maehyang' and examining the effect of seedling growth according to the IBA treatment method and concentration.
3 is a graph showing the number of runners (A), number of seedlings (B), runner length (C), and number of lateral buds (D) of strawberry 'maehyang' mother seed according to the IBA treatment method and concentration.
Figure 4 is the growth of strawberry 'maehyang' mother stock on the 64th day according to the IBA treatment method and concentration.
Figure 5 is a 64-day growth of strawberry 'Maehyang' runner and seedlings according to the IBA treatment method and concentration.

In order to achieve the object of the present invention, the present invention provides a method for inhibiting lateral embryogenesis and promoting the growth of seedlings, comprising the step of seedling seedlings by treating indole-3-butyric acid (IBA) in strawberry mother lines.

IBA is an auxin-based growth regulator, and auxin is a plant growth hormone that can suppress the growth of lateral buds while promoting the growth of spermatozoa through the dominance phenomenon. In addition to IBA, IAA (Indole-3-acetic acid), NAA (1-Naphthaleneacetic acid), and 2,4-D (2,4-Dichlorophenoxyacetic acid) are used as auxin-based growth regulators. Among them, IBA is IAA Unlike this, there is little decomposition by non-biological factors such as heat, so it has the advantage of stably exerting its effect in an external environment such as a greenhouse.

In the method of the present invention, the treatment of the IBA may be treated by a medium drenching method at a concentration of 30 to 120 mg·L ^-1 in the strawberry mother stock after planting, and more preferably in the strawberry mother stock after planting. It may be treated with a medium irrigation method 4 to 6 times at intervals of 2 weeks at a concentration of 80 to 120 mg L ^-1 , and more preferably 2 weeks at a concentration of 100 mg L ^-1 to the strawberry mother stock after planting. It may be treated with a medium irrigation method 5 times at intervals, but is not limited thereto.

When IBA is treated in the strawberry mother stock in the same way as described above, the occurrence of lateral buds is decreased compared to the IBA untreated group, and the number of seedlings and the length of the runners can be increased compared to the IBA untreated group. In addition, the growth of the tertiary seedlings generated from the mother line, i.e., leaf length, leaf length, leaf width, tube diameter, live and dry weight, showed superior characteristics compared to other treatment concentrations and foliar spray treatments.

In the method according to an embodiment of the present invention, the strawberry may be a 'Maehyang' variety ( Fragaria x ananassa Duch. cv. 'Maehyang'). Strawberries have been reported to show differences in response to growth regulators by cultivar due to differences in endogenous hormone levels, and Murti and Yeoung (2013, ARPN J. Agric. Biol. Sci. 8:405-410) reported that 'Camarosa It has been reported that ' and 'Redpearl' two varieties of strawberries were treated with different concentrations of IBA, resulting in differences in the growth of above-ground parts. Therefore, since the clear treatment concentration and treatment method of IBA may be different depending on the variety, it is necessary to find an appropriate concentration and treatment method.

The present invention also provides a composition for inhibiting the development of lateral buds and promoting the growth of seedlings during facility cultivation, containing 30-120 mg·L ^-1 of indole-3-butyric acid (IBA) as an active ingredient. The composition of the present invention is IBA adjusted to 30-120mg·L ^-1 , which is an optimal concentration condition that can improve the growth of tertiary seedlings generated from the mother line as well as the inhibitory effect on the lateral bud generation of the mother line during the seedling of 'Maehyang'. Since it contains as an active ingredient, productivity can be improved when the composition according to the present invention is applied to facility strawberry seedlings.

Hereinafter, the present invention will be described in detail by way of Examples. However, the following examples are only illustrative of the present invention, and the content of the present invention is not limited to the following examples.

Materials and Methods

1. Experimental materials and cultivation environment

This study was conducted in a plastic greenhouse in Gyeongnam region, and cocopeat Co. Ltd., Dummalasuriya, Srilanka) filled the facility strawberry 'Maehyang' ( Fragaria × ananssa Duch. cv. Maehyang) on April 12, 2019. It was planted in a pot (GS150, Goldstar Chemicals Co. Ltd., Chungju, Korea) with an outer diameter of 150 mm, a height of 135 mm, and an inner diameter of 90 mm, and cultivated for a total of 81 days until July 1, 2019. During the cultivation period, Gyeongsangnam-do Agricultural Research and Extension Services exclusive strawberry-only solution (major elements: NO ₃ ^- 13.0, NH ₄ ⁺ 1.0, H ₂ PO ₄ ^- 4.0, K ⁺ 6.0, Ca ²⁺ 8.0, Mg ²⁺ 4.0, SO ₄ ^2- 4.0 me L ^-1 , trace elements: Fe 3.0, B 0.5, Mn 0.5, Zn 0.2, Cu 0.04, Mo 0.04 mg L ^-1 , pH 6.5) per plant, 60 mL at a time, 5-6 per day by drip irrigation It was supplied in divided doses (3 minutes per session). For the management of the maternal line, the old leaves were removed periodically, and the lateral buds were removed at intervals of one week, and the number of lateral buds removed was recorded. Tribasic copper sulfate (15%), cyflufenamid (3.5%), abamectin (1.8%), imidacloprid ( 10%), acetamiprid (5%), and azoxystrobin (21.7%) were alternately sprayed with a cycle of 5-7 days. The average temperature and relative humidity inside the greenhouse where the cultivation experiment was conducted were 22±5℃ and 58±5%, respectively.

2. Treatment with growth regulators

IBA (Indole ^- 3-butyric acid, Sigma-Aldrich Co. Ltd., MO, USA) aqueous solution was treated in two ways: foliar spraying and medium irrigation at 50 mL per strawberry plant. Considering that IBA is naturally decomposed by heat and sunlight in a greenhouse environment, referring to the study of Aliyu et al. (2011, J. Crop. Sci. Biotechnol. 14:143-150), 2 weeks from the start date of treatment It was treated 5 times for a total of 9 weeks at intervals.

3. Investigation items

To compare the growth of 'Maehyang' strawberries according to the growth regulator treatment method and concentration, petiole length, leaf length, leaf width, leaf number, crown diameter, SPAD value, leaf area, and live weight of the above-ground part at 9 weeks after treatment with the growth regulator. The weight and dry weight, the number of lateral infants, the number of runners, the length of the runners, and the number of children were measured.

Petiole length, leaf length, and leaf width were measured based on the third leaf fully developed based on the growth point of the plant. The tube diameter was measured using a vernier caliper (CD-20CPX, Mitutoyo Co. Ltd., Kawasaki, Japan) at the top 1 cm of the paper part. The runner length was measured based on the primary runner. The SPAD value was measured from the third leaf fully developed based on the growth point using a chlorophyll meter (SPAD-502, Konica Minolta Inc., Tokyo, Japan). The leaf area was measured using a leaf area measuring device (LI-3000, LICOR Inc., NE, USA). Live weight and dry weight were measured using an electronic scale (EW220-3NM, Kern&Sohn GmbH., Balingen, Germany), and the dry weight was measured in a constant temperature dryer (Venticell-222, MMM Medcenter Einrichtungen GmbH., Planegg, Germany). It was measured after drying time. The electrical conductivity (EC) level and pH of the nutrient solution were measured using a portable pH/EC meter (Enzo 8200m, GOnDO Electronic Co. Ltd., Taipeo, Taiwan).

4. Statistical analysis

The experiment was arranged in a split gradient arrangement method of 4 repetitions of 6 subjects each. For statistical analysis, analysis of variance (ANOVA) was performed using the SAS program (SAS 9.4, SAS Institute Inc., Cary, NC, USA), and comparison between the means was performed at the 5% significance level using Duncan's multiple test. The significance of the liver was verified. The graph was shown using the SigmaPlot program (SigmaPlot 12.5, Systat Software Inc., CA, USA).

Example 1. Growth characteristics of 'Maehyang' strawberry mother seed according to IBA treatment method and concentration

As a result of analyzing the growth characteristics of 'Maehyang' strawberry mother seed according to the IBA treatment method and concentration, there was no significant difference in leaf length, leaf width, number of leaves, leaf area, fresh weight above ground and dry weight. However, the leaf blade length of the mother plant was significantly longer than that of the untreated control in all IBA-treated groups, and the tube diameter was measured to be the thickest in the experimental group treated with 50 mg·L ^-1 of IBA through medium irrigation (Table 1). Auxin can stimulate tissue differentiation and development by concentrating nutrients on cells according to the degree of accumulation in the tissue. Therefore, the irrigation-treated IBA permeated into the medium and was less exposed to light compared to the foliar spraying treatment, so compared to the foliar spraying treatment, the auxin was not decomposed and acted on the plant, and it was judged that the tube diameter of the parent plant became thicker. became There was no significant difference in SPAD value according to the concentration of IBA, but a higher value was measured in the medium irrigation treatment than in the foliar spray treatment.

The number of runners was the highest in the experimental group treated with 100 mg L ^-1 IBA by foliar spraying (Fig. 3A), and the total runner length was the experimental group treated with 150 mg L ^-1 IBA by foliar spraying. It was confirmed that in all IBA-treated groups except for an increase compared to the untreated control ( FIG. 3C ). In addition, the number of lateral follicles generated in mothers showed a tendency to decrease in the IBA-treated group compared to the untreated control group, and was observed to be the smallest in the experimental group treated with 100 mg·L ^-1 of IBA by foliar spraying (FIG. 3D). However, it was confirmed that the number of seedlings was reduced in all the experimental groups treated with IBA foliar spraying compared to the untreated control group (FIG. 3B), which was found that the foliar sprayed IBA was rapidly absorbed through the leaves compared to medium drench, thereby balancing the concentration of auxin in the plant. It was thought that the overall number of seedlings appeared to be low as the organ development of plants was inhibited by disintegration of the plant.

Example 2. Characteristics of seedling growth of 'Maehyang' strawberries according to IBA treatment method and concentration

As a result of analyzing the growth characteristics of the seedlings of 'Maehyang' strawberries according to the IBA treatment method and concentration, there was no significant difference in the above-ground growth of the primary seedlings according to the IBA treatment method and concentration (Table 2). The leaf length of the secondary seedlings was longer in the IBA-treated group than in the control group, and the tube diameter was the thickest in the control group (Table 3). The tube part of strawberry is where assimilation products are stored, and the accumulated assimilation products are consumed for the development and maintenance of tissues, and the development of storage organs may be reduced depending on the consumption amount. Considering that in the secondary seedlings, the growth of the petiole was less in the control compared to the IBA-treated seedlings, and the growth of the secondary seedlings and the tertiary seedlings connected by a runner was more active in the IBA-treated seedlings than in the control. , it was judged that the tube diameter was relatively large as the amount of assimilation products consumed in the control group was smaller than that of the seedlings treated with IBA.

The growth of the tertiary seedlings showed a tendency of high leaf length, leaf length, leaf width, leaf number, and leaf area in the treatment with medium irrigation 100 mg·L ^-1 (Table 4). Auxin promotes cell division in plants, thereby inducing organ elongation and development. Therefore, the height of leaves and petioles observed in the IBA-treated group compared to the control group was judged to be due to the effect of auxin. There was no significant difference in the live weight and dry weight of the primary and secondary seedlings between the treatments, and the live weight of the tertiary seedlings was the heaviest in the medium drench 100 mg·L ^-1 treatment, and the dry weight was 100 mg compared to the control. ·L ^-1 treatment and foliar application 200 mg·L ^-1 treatment were significantly heavier (Table 5).

Summarizing the above results, there was no significant difference in the growth of plant strawberry 'Maehyang' according to the treatment method and concentration of ^IBA in the mother stock, 1st and 2nd seedlings. It was confirmed that it can be improved through medium irrigation treatment. Therefore, considering the suppression of lateral embryogenesis and the growth of tertiary seedlings, it is judged that the appropriate IBA treatment method and concentration is to treat IBA 100 mg·L ^-1 to 'Maehyang' strawberries when grown in a facility as a medium irrigation method. did.

Claims (7)

Strawberry 'Maehyang' ( Fragaria x ananassa Duch. cv. 'Maehyang') After planting 100 mg L ^-1 concentration of IBA (indole-3-butyric acid) in the mother stock, 4 to 6 times at an interval of 2 weeks. A method for inhibiting the development of lateral buds of strawberry 'maehyang' mother seedlings and enhancing the live and dry weight of tertiary seedlings, comprising the step of seedling by treatment with drench. delete delete delete delete delete delete

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