KR20200107373A - Feed additive composition and animal feed comprising said composition - Google Patents

Abstract

The present invention relates to an animal feed. More particularly, the present invention relates to a feed additive composition capable of improving stress tolerance and biological immunity of animals while increasing digestibility of nutrients by maintaining the microecological balance in the organs of animals, and to an animal feed comprising the composition. The feed additive composition includes N-carbamoyl glutamic acid and probiotics as active ingredients.

Description

Feed additive composition and animal feed comprising said composition

The present invention relates to an animal feed, and more specifically, a feed additive composition capable of improving the digestibility of nutrients (digestibility) by maintaining the microecological balance in the organs of the animal while improving the stress tolerance and biological immunity of the animal, and the above It relates to animal feed containing the composition.

As pathogens that cause intestinal infections in animals, pathogenic E. coli, Salmonella, Clostridium, Campylobacter, and the like are known. Such pathogens are known to produce toxins (enterotoxins, cytotoxins) when abnormally proliferated, and cause disorders in the intestinal mucosa, causing soft stool (軟便) and severe diarrhea. In addition, coccidium is a parasitic protozoan in the intestines such as chickens, pigs, and cattle, and when infected, it causes diarrhea and loss of appetite. Antimicrobial substances are used to prevent or treat such inflammatory bowel disorders, but there are problems such as the appearance of drug-resistant bacteria.

Recently, in order to improve the balance of flora in the intestine and suppress the proliferation of pathogens in the intestine, a technology using probiotics has attracted attention. However, lactic acid bacteria and bifidobacteria are often killed at a concentration of 0.3% deoxycholic acid or at a pH of 4 or less. Except for bacteria collectively referred to as the coliform group, deoxycholic acid having strong antibacterial properties against microorganisms in bile acids There are many species that cannot survive in their presence. Therefore, there is a demand for a fungus species that does not die even in the digestive tract of an animal and has a beneficial effect on the host.

On the other hand, it has been reported that the technology of reducing the gastrointestinal burden of animals by treating animal feed with enzymes or enzyme-producing bacteria at the processing stage and decomposing to some extent is useful for preventing or improving digestive system diseases. have. In addition, there is known a method of fermenting fish meal in low moisture using koji bacteria to obtain a fish meal fermented feed for fish containing a high concentration of protease, lipase, and the like. However, in such a technique, there is a problem that a cumbersome process of increasing the moisture content in the feed to decompose the components of the feed and then drying and commercializing it is required. In addition, the feed with increased moisture content for component decomposition also has problems such as spoilage, mold, etc., and it is difficult to maintain quality.

Therefore, there is a need to develop a new composition of feed additives to solve this problem.

Domestic Patent Publication No. 10-1009-0053927

As a result of a number of studies, the present inventors completed the present invention by developing a feed additive composition of a new composition through the synergistic effect of N-carbamoyl glutamic acid and probiotics.

Accordingly, an object of the present invention is to increase the digestibility of nutrients by maintaining the balance of the microbial ecosystem in the intestine of animals through the synergistic action of N-carbamoyl glutamic acid and probiotics, to increase the biological immunity of animals, and to improve the productivity of animals. It is to provide a feed additive composition of a new composition that can be made and animal feed containing the composition.

Another object of the present invention is that production cost is low, production is easy, and it is easy to add, so that not only can the animal breeding cost be lowered, but also the content of harmful gases in the breeding environment is lowered, stable manure of animals, residual feed and organic matter. It is to provide a feed additive composition that can improve the breeding environment by decomposing decay substances and animal feed containing the composition.

The object of the present invention is not limited to the above-mentioned object, and even if not explicitly mentioned, the object of the invention that one of ordinary skill in the art can recognize from the description of the detailed description of the invention to be described later may naturally be included. .

In order to achieve the object of the present invention described above, the present invention provides a feed additive composition comprising N-carbamoyl glutamic acid and probiotics as active ingredients.

In a preferred embodiment, the probiotics include at least one of lactic acid bacteria, Enterococcus faecalis, and yeast.

In a preferred embodiment, the N-carbamoyl glutamic acid and lactic acid bacteria are included in a weight ratio of 1:3-10.

In a preferred embodiment, the N-carbamoyl glutamic acid, lactic acid bacteria, and Enterococcus pecalis bacteria are included in a weight ratio of 3:8-15:3-8. Feed additive composition, characterized in that the.

In a preferred embodiment, the N-carbamoyl glutamic acid, lactic acid bacteria, Enterococos pecalis bacteria, and yeast bacteria are included in a weight ratio of 3:8-15:3-8:0.5-3.

In addition, the present invention provides an animal feed comprising any one of the above-described feed additive composition and feed base.

In a preferred embodiment, the feed base is a completely blended feed.

In a preferred embodiment, the feed additive composition is contained in an amount of 2.5 to 7.5 parts by weight per 100 parts by weight of the feed base.

In a preferred embodiment, the feed additive composition improves one or more of animal productivity, bioimmunity, and anti-stress activity.

According to the animal feed containing the feed additive composition and the composition of the present invention described above, the digestibility of nutrients is increased by maintaining the balance of the microbial ecosystem in the intestine of the animal through the synergy between N-carbamoyl glutamic acid and probiotics, and the animal It increases the immunity of the body and can improve the productivity of animals.

In addition, according to the feed additive composition of the present invention and the animal feed containing the composition, the production cost is low, production is easy, and the addition is easy to reduce the cost of raising animals, as well as lowering the content of harmful gases in the breeding environment. , Animal feces (stable manure), residual feed and organic decay substances can be decomposed to improve the breeding environment.

These technical effects of the present invention are not limited only to the ranges mentioned above, and even if not explicitly mentioned, the effects of the invention that can be recognized by those of ordinary skill in the art from the description of specific details for the implementation of the invention described later are also Of course it is included.

The terms used in the present invention are only used to describe specific embodiments, and are not intended to limit the present invention. Singular expressions include plural expressions unless the context clearly indicates otherwise. In the present application, terms such as "comprise" or "have" are intended to designate the existence of features, numbers, steps, actions, elements, parts, or a combination thereof described in the description of the invention, but one or more other It is to be understood that it does not preclude the presence or addition of features, numbers, steps, actions, components, parts, or combinations thereof.

Terms such as first and second may be used to describe various elements, but the elements should not be limited by the terms. These terms are only used for the purpose of distinguishing one component from another component. For example, without departing from the scope of the present invention, a first component may be referred to as a second component, and similarly, a second component may be referred to as a first component.

Unless otherwise defined, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which the present invention belongs. Terms as defined in a commonly used dictionary should be interpreted as having a meaning consistent with the meaning in the context of the related technology, and should not be interpreted as an ideal or excessively formal meaning unless explicitly defined in the present invention. Does not.

In interpreting the constituent elements, it is interpreted as including an error range even if there is no explicit description. In particular, when the terms "about", "substantially" and the like of degree are used, it can be interpreted as being used in or close to that value when manufacturing and material tolerances specific to the stated meaning are presented. .

In the case of a description of a temporal relationship, for example,'after','following','after','before', etc. It includes cases that are not continuous unless' is used.

Hereinafter, the technical configuration of the present invention will be described in detail with reference to the accompanying drawings and preferred embodiments.

However, the present invention is not limited to the embodiments described herein and may be embodied in other forms. The same reference numerals used to describe the present invention throughout the specification denote the same elements.

The technical feature of the present invention is that N-carbamoyl glutamic acid maintains the balance of the microbial ecosystem in the intestine of animals, and in particular, increases the digestibility of nutrients by generating synergy with probiotics, and also increases the biological immunity of animals, and breeding environment There is a new use of N-carbamoyl glutamic acid to purify the breeding environment by lowering the content of harmful gases and decomposing animal feces (stable manure), residual feed and organic decay substances.

That is, N-carbamoyl glutamic acid is a metabolic stabilizing analog of N-acetylglutamic acid, and it activates the restriction of the endogenous synthesis of arginine. This is because it improves endogenous synthesis and maintains the arginine <Thionine> in the body at a high physiological level. In other words, arginine can be converted into substances with important biological activities such as NO and doamines (including butamine, acetylene and thiamine) through a series of metabolic processes in the body, and these substances are nutrient metabolism and immune response in the body. Because it happens in the field.

Therefore, the feed additive composition of the present invention contains N-carbamoyl glutamic acid and probiotics as active ingredients.

Example 1

Feed additive 1 (Gunk 1) was obtained by uniformly mixing 1 kg of N-carbamoyl glutamic acid and 3 kg of lactic acid bacteria. Here, the colony-forming unit (cfu) of lactic acid bacteria is 10 (cfu/g).

Example 2

Feed additive 2 (Gunk 2) was obtained by uniformly mixing 3 kg of N-carbamoyl glutamic acid, 8 kg of lactic acid bacteria and 3 kg of Enterococose pecalis bacteria. Here, the colony forming unit of each probiotic is 10 (cfu/g).

Example 3

Feed additive 3 (Gunk 3) was obtained by uniformly mixing 3 kg of N-carbamoyl glutamic acid, 8 kg of lactic acid bacteria, 5 kg of Enterococose pecalis, and 2 kg of yeast. Here, the colony forming unit of each probiotic is 10 (cfu/g).

Example 4

An animal feed was obtained by uniformly mixing 0.5 kg of feed additive 3 (Gunk 3) obtained in Example 3 per ton of completely blended feed.

Comparative Example 1

Comparative animal feed 1 was obtained in the same manner as in Example 4, except that N-carbamoyl glutamic acid was used instead of feed additive 3.

Comparative Example 2

Comparative animal feed 2 was obtained in the same manner as in Example 4, except that the complex probiotics having the same composition as in Example 3 were used instead of feed additive 3.

Experimental Example 1

The effect of N-carbamoyl glutamic acid (NCG) on pig productivity was tested as follows, and the results are shown in Table 2. In the present invention, the productivity (performance) specifically means a growth rate or a feed conversion rate (feed conversion rate).

Three-way cross-wet piglets of similar weight were selected, and 160 pigs were randomly divided into four groups, and each of 40 animals was repeated 4 by 10. The treatment given in Table 1 is performed at random per group, and the breeding steps and additives added per group are as shown in Table 1, NCG is added to the perfect compound feed, and in Table 1 NCG is additionally added to the full price compound feed. Added, for example, 0.25 kg/ton NCG represents an additional 0.25 kg per ton of full-price blended feed.

division Breeding stage Additive amount of feed additive Experiment A Childcare stage 0.25kg/ton NCG Experiment B Childcare stage 0.50kg/ton NCG Experiment C Childcare stage 0.75kg/ton NCG Control Childcare stage No NCG added

Each group is constantly managed for each pig farm, and each group is fed the appropriate daily food, divided into a preliminary investigation period for about a week, and adaptive cultivation is performed, vaccination and insect repellent are combined, and then free choice ), drink water freely, and perform immunization according to normal pig farm procedures.

The experiment period started on September 5, 2018, and the experiment period was 25 days.

On the start date of the experiment period, the experiment was started by observing the feeding status of each pig and the pig's health status every day. The fasting and body weight at the end of the experiment are called, and the daily gain (ADG), daily intake (ADFI), and feed conversion rate (F/G) are calculated. Among these, feed conversion rate (F/G) = daily intake (ADFI)/daily gain (ADG).

Initial weight
/kg At the end
Weight/kg charge per day
Weight gain/gd ^-1 Daily vegetarian diet/gd ^-1 Feed conversion rate (F/G) Experiment A 9.68±2.01 21.08±2.87 ^b 456.00±20.54 ^b 814.99±59.13 1.79:1 Experiment B 9.67±1.86 21.92±2.75 ^b 490.00±20.41 ^b 816.40±55.56 1.67:1 Experiment C 9.67±2.00 21.14±2.31 ^b 458.00±21.38 ^c 814.23±58.11 1.77:1 Control 9.71±1.92 20.53±3.01 ^a 432.00±24.25 ^a 806.79±59.95 1.86:1

(Different lowercase letters in the same column indicate significant differences (p003c#0.05))

Table 2 shows that when NCG is added to feed in the incubation stage, the productivity is better than that of the control group, and that adding 0.50kg/t NCG is the most effective. Compared to the control group, the feed conversion rates of Experiments A, B and C were improved by 3.76%, 10.21% and 4.84%, respectively.

Experimental Example 2

The effect of N-carbamoyl glutamic acid (NCG) on the serum index of pigs was tested as follows, and the results are shown in Table 3.

On the day of the end of the experiment period of Experimental Example 1, one pig per group was randomly selected (random selection), and the serum was collected through 8 mL of a precava, and after serum precipitation, centrifugation at 3000 r/min for 10 minutes. Serum content of Baekgubi, malondialdehyde (MDA), superoxide dismutase (SOD), halftoglobin (haptoglobin), and interferon-γ (IFN-γ) growth hormone (GH) in Beijing Measurements were made using the corresponding reagent cartridge provided by Dongga Biological Technology Co., Ltd.

(Different lowercase letters in the same column indicate that the difference is significant (p003c#0.05), and other uppercase letters indicate that the difference is very significant (p003c#0.01))

Table 3 shows that the white mouth ratio of each experimental group was significantly lowered compared to the control group (p003c#0.05) and the experimental B group was very markedly lowered (p003c#0.01). Porcine halftoglobin, an immunological parameter for each experimental team, was significantly lower than that of the control group (p003c#0.05). In addition, interferon-γ (IFN-γ) in each experimental group was improved than that of the control group, and significantly improved in experimental group B (p003c#0.05). The improvement in the biochemical and immune parameters of each experimental team compared to the control shows that the gas resistance and immunity of pigs in the experimental group increased after adding NCG. In addition, growth hormone in each experimental group is increasing compared to the control group, and among them, it can be seen that the experimental B group (that is, 0.50kg/t NCG added) is the most effective.

Experimental Example 3

The effect of the synergistic effect of N-carbamoyl glutamic acid (NCG) and complex probiotics on the performance of pigs was tested as follows, and the results are shown in Table 5.

Three-way hybrids with similar weights Select 160 weaned piglets and divide them into four groups at random, repeating four for four and ten for each. The random treatment in Table 4 is performed at random per group, and the breeding steps per group, the type and amount of additives added are for animals prepared by adding NCG and/or complex probiotics to the perfect compound feed as shown in Table 4. Feed, feed for comparative animals 1 and 2 were used.

Each group is managed by pig farms at all times, each group is fed the food for the day, and the test period is set over a week for adaptive cultivation, vaccination and insect repelling are performed, then enter the laboratory to eat freely and water freely. They were immunized according to the normal procedures of the pig farm while drinking. The experiment period was conducted for 25 days from October 8, 2018.

division Breeding stage Type and amount of feed additives Experiment A Childcare stage 0.5kg/ton NCG (Comparative Animal Feed 1) Experiment B Childcare stage 0.5kg/ton Complex Probiotics (Comparative Animal Feed 2) Experiment C Childcare stage 0.5kg/ton NCG + complex probiotics (feed for animals) Control Childcare stage No feed additives added

After the start of the experiment period, the daily intake of the pig and the health of the pig are observed every day, and the body weight at the beginning and end of the experiment is measured to record the daily intake, and daily gain (ADG), daily intake (ADFI) and feed The feed conversion rate (F/G) was calculated. Among these, feed conversion rate (F/G) = daily intake (ADFI)/daily gain (ADG).

(Different lowercase letters in the same column indicate significant differences (p003c#0.05))

Table 5 shows that the combination of NCG, complex probiotics, NCG and complex probiotics added to the incubation stage feed, i.e., comparative animal feeds 1 and 2, and animal feeds, have better production performance than the control group. That is, compared to the control group, Experiment A increased by 6.45%, Group B by 5.91%, and Group C by 11.83%. These results show that NCG has a synergistic effect when used with complex probiotics.

Experimental Example 4

The effect of the synergistic effect of N-carbamoyl glutamic acid (NCG) and complex probiotics on the serum index of pigs was tested as follows, and the results are shown in Table 6.

On the day of the end of the experiment period of Experimental Example 3, one pig was randomly selected for each group and tilted at an angle of 8 mL in the anterior vein, followed by centrifugation at 3000r/min for 10min after serum precipitation, and the serum was collected in an Ep tube and cooled at -20°C. In the same manner as in Experimental Example 2, the white mouth ratio was measured in serum, and protoglumine (MDA), superoxide dismutase (SOD), halftoglobin-globin, interferon-γ (IFN-γ) and growth The content of growth hormone (GH) was measured.

(Different lowercase letters in the same column indicate that the difference is significant (p003c#0.05), and other uppercase letters indicate that the difference is very significant (p003c#0.01))

Table 6 shows that the white mouth ratio of each experimental group was all lowered compared to the control group (p003c#0.05). In particular, it can be seen that the experiment C group fed the animal feed of the present invention significantly decreased (p003c#0.01). It can also be confirmed that the pig-binding protein (haptoglobin), an immune indicator for each experimental group, is significantly lower than that of the control group (p003c#0.05). Interferon-γ (IFN-γ) in each experimental group was all improved compared to the control group, and in particular, the experimental group C was significantly improved (p003c#0.05). In addition, it can be seen that the biochemical markers and immune parameters of each experimental group are improving compared to the control group. In addition, compared to the control team, growth hormone in each experimental group showed a higher trend. These experimental results show that the anti-stress and immunity of pigs in each experimental group were improved. In particular, animal feed containing NCG and complex probiotics used in Experiment C increased feed utilization, promoted animal growth, and It can be seen that it was most effective in increasing stress ability and gas immunity.

Although the present invention has been illustrated and described with a preferred embodiment as described above, it is not limited to the above-described embodiment, and within the scope of the spirit of the present invention, those of ordinary skill in the art Various changes and modifications will be possible.

Claims (9)

A feed additive composition containing N-carbamoyl glutamic acid and probiotics as active ingredients.
The method of claim 1,
The probiotic is a feed additive composition comprising at least one of lactic acid bacteria, Enterococcus faecalis, and yeast.
The method of claim 2,
The feed additive composition, characterized in that the N-carbamoyl glutamic acid and lactic acid bacteria are contained in a weight ratio of 1: 3-10.
The method of claim 2,
The feed additive composition, characterized in that the N-carbamoyl glutamic acid, lactic acid bacteria, Enterococcus pecalis bacteria are contained in a weight ratio of 3:8-15:3-8.
The method of claim 2,
The feed additive composition, characterized in that the N-carbamoyl glutamic acid, lactic acid bacteria, Enterococos pecalis bacteria, yeast bacteria are included in a weight ratio of 3:8-15:3-8:0.5-3.
Animal feed comprising the feed additive composition and feed base of any one of claims 1 to 5.
The method of claim 6,
Animal feed, characterized in that the feed base is a complete blended feed.
The method of claim 7,
The feed additive composition is animal feed, characterized in that contained in an amount of 2.5 to 7.5 parts by weight per 100 parts by weight of the feed base.
The method of claim 7,
The feed additive composition is an animal feed, characterized in that to improve one or more of the productivity, bioimmunity and anti-stress activity of the animal.